CcUC08G148680 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC08G148680
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	rRNA N-glycosidase
Location	CicolChr08: 13153146 .. 13154069 (+)
RNA-Seq Expression	CcUC08G148680
Synteny	CcUC08G148680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGGAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGAAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGCTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACAGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCTTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTAATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAACCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGTGGAAACGGACAGTTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAAGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATCACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAACAAATTCTGGCTACAAAAAGGAGAGCAAATTGGTTCAGCTATCAATATGA

mRNA sequence

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGGAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGAAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGCTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACAGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCTTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTAATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAACCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGTGGAAACGGACAGTTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAAGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATCACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAACAAATTCTGGCTACAAAAAGGAGAGCAAATTGGTTCAGCTATCAATATGA

Coding sequence (CDS)

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGGAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGAAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGCTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACAGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCTTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTAATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAACCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGTGGAAACGGACAGTTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAAGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATCACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAACAAATTCTGGCTACAAAAAGGAGAGCAAATTGGTTCAGCTATCAATATGA

Protein sequence

MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVLPSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTDTRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITPNGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSMLHPGAIMSPTNSGYKKESKLVQLSI

Homology

[Close]

BLAST of CcUC08G148680 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-106
Identity = 204/284 (71.83%), Postives = 238/284 (83.80%), Query Frame = 0


Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAY A AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD+IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN +G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVK NIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

[Close]

BLAST of CcUC08G148680 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 391.3 bits (1004), Expect = 7.4e-105
Identity = 209/289 (72.32%), Postives = 238/289 (82.35%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           MMKSIA+ FFI+ IF+G PT KGDVSF L TAT  SYTSFI  LR+ALPT G VCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGLQWF FFSLTNYN +TITVAVNVTNVYI AY  GAVSYFFEDTSAEA+ LLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFY-YDYKPTAAALLVLIQST 180
           T+KV LPYSGNYDRLQ++VGK RDLIELG P LS+AITN+FY  +YK TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLIT 240
           AEAARFKYIEQ++S++++ NF P+ AIISLEN+WGALSKQIQIANS G GQF+  ++LIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            +G RVSVTNTS GVVKSNIKLLL+Y     V   + + I ++L  GAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYY----KVNGANDNYIPTILPLGAI 285

[Close]

BLAST of CcUC08G148680 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 379.8 bits (974), Expect = 2.2e-101
Identity = 199/282 (70.57%), Postives = 237/282 (84.04%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F+VAI +  PT+K D++F L TAT  SY++FI QLRNALPTT  VCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGL WFTFF++TNYN +TITVAVNVTNVYIVAY A A SYFFEDT AEA  LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RDLIELG PALS+A+TNM YY+ + TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIEQQ++Q+I+  F P+ A+ISLENNWGALSKQIQIANS+G GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR SVT+TSAGVV+SNIKLLL+Y L    VAD+ D  T M
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKL---SVADENDSPTRM 279

[Close]

BLAST of CcUC08G148680 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 4.7e-99
Identity = 191/282 (67.73%), Postives = 234/282 (82.98%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++F L TAT  SY++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL WFTFF++TNYN +T+ VAVNV+NVYIVAY A A SYFFEDT A+A  LLF  
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RD++ELG PALS+A+TNM YY+ +PTAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS+G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+ NIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CcUC08G148680 vs. NCBI nr
Match: XP_022960460.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 2.2e-96
Identity = 191/282 (67.73%), Postives = 231/282 (81.91%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++  L TAT  +Y++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL  FTFF++TNYN ET+ VAVNV+NVYIVAY A A SYFFEDTSA+A  LLF +
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+K+ LP++GNYDRLQ VVGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS+G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+ NIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-109
Identity = 204/284 (71.83%), Postives = 238/284 (83.80%), Query Frame = 0


Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAY A AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD+IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN +G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVK NIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

[Close]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 285.0 bits (728), Expect = 9.8e-76
Identity = 155/282 (54.96%), Postives = 202/282 (71.63%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAY    VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A + G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVK NIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

[Close]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-75
Identity = 155/282 (54.96%), Postives = 201/282 (71.28%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F    LT+Y  ETI+VA++VTNVY+VAY    VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A + G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVK NIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

[Close]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-75
Identity = 164/276 (59.42%), Postives = 196/276 (71.01%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + ++  I+ IF+  PTV+GDVSF L  AT  SY  FI  LR ALP   KV NIP+L
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSA-EAWNLLFT 120
            S+ SG   +T   LTNY DETI+VAV+VTNVYI+ YLAG VSYFF + SA EA   +F 
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 DT-RKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D  +KV LPYSGNY+RLQ   GK R+ I LG PAL SAIT ++YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLI 240
           TAE+AR+K+IEQQI + ++  FLP LA ISLENNW ALSKQIQIA S+ NGQFESP+ LI
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVAD 275
             N  RVS+TN SA VV SNI LLL  N +A +  D
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

[Close]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 274.6 bits (701), Expect = 1.3e-72
Identity = 157/283 (55.48%), Postives = 195/283 (68.90%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M++ + +   I+ +F+  P V+GDVSF L  AT  SY  FI  LR ALP   K+ +IP+L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSA-EAWNLLFT 120
            S+  G Q +    LTNY DETI+VA++VTNVYI+ Y AG  SYFF + SA EA   +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DT-RKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D  RKV LPYSGNY+RLQ   GK R+ I LG PAL SAIT +FYY+    A+AL+VLIQS
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLI 240
           T+EAAR+K+IEQQI + ++  FLP LAIISLEN+W ALSKQIQIA S+ NGQFESP+ LI
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITS 282
                RV++TN  AGVV SNI LLL  N +A  + DD  M  S
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMA-AMDDDVPMTQS 281

[Close]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.6e-105
Identity = 209/289 (72.32%), Postives = 238/289 (82.35%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           MMKSIA+ FFI+ IF+G PT KGDVSF L TAT  SYTSFI  LR+ALPT G VCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGLQWF FFSLTNYN +TITVAVNVTNVYI AY  GAVSYFFEDTSAEA+ LLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFY-YDYKPTAAALLVLIQST 180
           T+KV LPYSGNYDRLQ++VGK RDLIELG P LS+AITN+FY  +YK TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLIT 240
           AEAARFKYIEQ++S++++ NF P+ AIISLEN+WGALSKQIQIANS G GQF+  ++LIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            +G RVSVTNTS GVVKSNIKLLL+Y     V   + + I ++L  GAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYY----KVNGANDNYIPTILPLGAI 285

[Close]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.1e-101
Identity = 199/282 (70.57%), Postives = 237/282 (84.04%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F+VAI +  PT+K D++F L TAT  SY++FI QLRNALPTT  VCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGL WFTFF++TNYN +TITVAVNVTNVYIVAY A A SYFFEDT AEA  LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RDLIELG PALS+A+TNM YY+ + TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIEQQ++Q+I+  F P+ A+ISLENNWGALSKQIQIANS+G GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR SVT+TSAGVV+SNIKLLL+Y L    VAD+ D  T M
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKL---SVADENDSPTRM 279

[Close]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 1.0e-96
Identity = 191/282 (67.73%), Postives = 231/282 (81.91%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++  L TAT  +Y++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL  FTFF++TNYN ET+ VAVNV+NVYIVAY A A SYFFEDTSA+A  LLF +
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+K+ LP++GNYDRLQ VVGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS+G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+ NIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 7.5e-87
Identity = 173/268 (64.55%), Postives = 209/268 (77.99%), Query Frame = 0


Query: 2   MKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVLP 61
           M+ +     ++AI +G  + +GDVSF L  A  KSYT+FI QLR+A+PTT  VC IPVLP
Sbjct: 1   MRKLTTLSLLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVLP 60

Query: 62  STASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTDT 121
           STASG + FTF  LTNY D+T+ VA+N+TNVYIVAY    VSYFF+D S EA+ +LF  T
Sbjct: 61  STASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEGT 120

Query: 122 RKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAE 181
            +  LPYS NYD+LQ++VGK+RDLIELG PALSSAITN+ YY+YK TAAALLVLIQS AE
Sbjct: 121 DRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYYEYKSTAAALLVLIQSVAE 180

Query: 182 AARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITPN 241
           AAR+KYIEQ+IS +++  F PD AIISLENNWGALSKQIQIA + GNGQFE P++LI PN
Sbjct: 181 AARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELINPN 240

Query: 242 GTRVSVTNTSAGVVKSNIKLLLFYNLLA 270
            T  SVTN S+ VV SNIKLLL++ L A
Sbjct: 241 NTPFSVTNVSSLVVTSNIKLLLYFKLAA 267

[Close]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL
Match: Q0ZBB8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 7.3e-74
Identity = 156/282 (55.32%), Postives = 202/282 (71.63%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATGKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYLAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAY    VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRHFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDLIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSSGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A + G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKSNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVK NIKLLL  N  A+    D D IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DEDFITTM 277

The following BLAST results are available for this feature:

BLAST of CcUC08G148680 vs. NCBI nr


Match Name	E-value	Identity	Description
P56626.2	1.0e-106	71.83	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_022156709.1	7.4e-105	72.32	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]
XP_023005055.1	2.2e-101	70.57	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
KAG6592904.1	4.7e-99	67.73	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]
XP_022960460.1	2.2e-96	67.73	type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata]	[more]

BLAST of CcUC08G148680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	1.4e-109	71.83	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	9.8e-76	54.96	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.3e-75	54.96	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P33185	1.7e-75	59.42	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P09989	1.3e-72	55.48	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC7	3.6e-105	72.32	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1KWC4	1.1e-101	70.57	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1H951	1.0e-96	67.73	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	7.5e-87	64.55	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
Q0ZBB8	7.3e-74	55.32	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1	[more]

BLAST of CcUC08G148680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 35..48 score: 44.17 coord: 82..97 score: 52.63 coord: 172..192 score: 66.29 coord: 204..221 score: 53.51 coord: 143..157 score: 49.82
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 1.6E-43 score: 150.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 34..221 e-value: 2.0E-31 score: 109.2
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 25..257
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..268 e-value: 1.0E-28 score: 101.1
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..257
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC08G148680.1	CcUC08G148680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity