CcUC07G144730 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC07G144730
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	CicolChr07: 30888416 .. 30889100 (-)
RNA-Seq Expression	CcUC07G144730
Synteny	CcUC07G144730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTATAAAAACTTATTGAAACATTTGGATTGTTCTAGGTTCTTATTTCATAAGAAACTAATATCTTTGCAAGTTGAATTTGTTTGCAGCAATCTACTCGATGAATGTGGAAGTATAATGACAGAACTATCAAGAAAAATAATGAAGCTATTACTAGGAGATAATTTTGAGAACAAATTCTATGAGTCAGAGTTCAGTAAGTGCAATGGCTACATAAGAATAAATAGATACGCTCCTCGAAATTCCAACAAAGAAATCGAAGCGTTTGGGAAGCACACCGACATAAGTTGTGTGACGATTCTATTCCAAGACGAAATCGGGGGCCTTCAAATGAAATCGAAACGAAGAGATGAGTGGATAGATGTAAAACCTCTAGAGGATGCTCTTTTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGTAATGAAAGGCTAAGATCAGCTGAGCATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTGGACATTTGGGGATGATGATAGGGAAGTATATGCCCCACGTGAGGTTGTTGGAGAAGGAAATACGAGGCTTTATAAGCCATTTTCAACTAAAGAATACAGGGCGTTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATCAGAGAATTCGCCGGAGTTCTAGAATCACAGCATTAA

mRNA sequence

ATGAAGAAACTAATATCTTTGCAAGTTGAATTTGTTTGCAGCAATCTACTCGATGAATGTGGAAGTATAATGACAGAACTATCAAGAAAAATAATGAAGCTATTACTAGGAGATAATTTTGAGAACAAATTCTATGAGTCAGAGTTCAGTAAGTGCAATGGCTACATAAGAATAAATAGATACGCTCCTCGAAATTCCAACAAAGAAATCGAAGCGTTTGGGAAGCACACCGACATAAGTTGTGTGACGATTCTATTCCAAGACGAAATCGGGGGCCTTCAAATGAAATCGAAACGAAGAGATGAGTGGATAGATGTAAAACCTCTAGAGGATGCTCTTTTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGTAATGAAAGGCTAAGATCAGCTGAGCATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTGGACATTTGGGGATGATGATAGGGAAGTATATGCCCCACGTGAGGTTGTTGGAGAAGGAAATACGAGGCTTTATAAGCCATTTTCAACTAAAGAATACAGGGCGTTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATCAGAGAATTCGCCGGAGTTCTAGAATCACAGCATTAA

Coding sequence (CDS)

ATGAAGAAACTAATATCTTTGCAAGTTGAATTTGTTTGCAGCAATCTACTCGATGAATGTGGAAGTATAATGACAGAACTATCAAGAAAAATAATGAAGCTATTACTAGGAGATAATTTTGAGAACAAATTCTATGAGTCAGAGTTCAGTAAGTGCAATGGCTACATAAGAATAAATAGATACGCTCCTCGAAATTCCAACAAAGAAATCGAAGCGTTTGGGAAGCACACCGACATAAGTTGTGTGACGATTCTATTCCAAGACGAAATCGGGGGCCTTCAAATGAAATCGAAACGAAGAGATGAGTGGATAGATGTAAAACCTCTAGAGGATGCTCTTTTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGTAATGAAAGGCTAAGATCAGCTGAGCATAGAGTTGTTTTGAAGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTGGACATTTGGGGATGATGATAGGGAAGTATATGCCCCACGTGAGGTTGTTGGAGAAGGAAATACGAGGCTTTATAAGCCATTTTCAACTAAAGAATACAGGGCGTTTAGGGAAAATAATTATGGGAAAATTGTTGGAACCCCAATCAGAGAATTCGCCGGAGTTCTAGAATCACAGCATTAA

Protein sequence

MKKLISLQVEFVCSNLLDECGSIMTELSRKIMKLLLGDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNYGKIVGTPIREFAGVLESQH

Homology

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BLAST of CcUC07G144730 vs. NCBI nr
Match: XP_038876311.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 364.4 bits (934), Expect = 6.7e-97
Identity = 178/194 (91.75%), Postives = 183/194 (94.33%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEI 73
           SNLLDE GSIM ELSRKIMKLLL   GDN ENKFYESEFSKCNGYIRINRYAPRNSN+EI
Sbjct: 122 SNLLDEYGSIMIELSRKIMKLLLKIMGDNLENKFYESEFSKCNGYIRINRYAPRNSNEEI 181

Query: 74  EAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLR 133
           EAFGKHTDISCVTILFQDEIGGLQMKSK+ DEWIDVKPLEDALLVNIGDFMQAWSNERLR
Sbjct: 182 EAFGKHTDISCVTILFQDEIGGLQMKSKQGDEWIDVKPLEDALLVNIGDFMQAWSNERLR 241

Query: 134 SAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENN 193
           SAEHRVVLKQNVKRFSLAFF  FGDDDRE+YAP EVVGEGNTRLYKPFSTKEYRAFRE N
Sbjct: 242 SAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFREYN 301

Query: 194 YGKIVGTPIREFAG 205
           YGK+VGTP+REFAG
Sbjct: 302 YGKVVGTPLREFAG 315

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BLAST of CcUC07G144730 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 349.0 bits (894), Expect = 2.9e-92
Identity = 165/194 (85.05%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 15  NLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIE 74
           NLLDE GSIM ELSR+IMKLLL   GD+FE+KFYESEFS CNGYIRINRYAPRNSN+EIE
Sbjct: 126 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE 185

Query: 75  AFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRS 134
           AFGKHTDISCVTILFQDEIGG+QMKSK+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRS
Sbjct: 186 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 245

Query: 135 AEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNY 194
           AEHRVVLKQN KRF+L FF  FGDDDRE+YAP EVVGEGN R+YKPFSTKEYRAFRENNY
Sbjct: 246 AEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 305

Query: 195 GKIVGTPIREFAGV 206
           GK+VG P+REFAG+
Sbjct: 306 GKVVGVPMREFAGI 319

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BLAST of CcUC07G144730 vs. NCBI nr
Match: KAA0044653.1 (gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 345.9 bits (886), Expect = 2.4e-91
Identity = 163/194 (84.02%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 15  NLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIE 74
           NLLDE GSIM ELSR+IMKLLL   GD+FE+KFYESEFS CNGYIRINRYAPRNSN+EIE
Sbjct: 126 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE 185

Query: 75  AFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRS 134
           AFGKHTDISCVTILFQDEIGG+QMKSK+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRS
Sbjct: 186 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 245

Query: 135 AEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNY 194
            EHRVVLKQN +RF+L FF  FGDDDRE+YAP EVVGEGN R+YKPFSTKEYRAFRENNY
Sbjct: 246 TEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 305

Query: 195 GKIVGTPIREFAGV 206
           GK+VG P+REFAG+
Sbjct: 306 GKVVGVPMREFAGI 319

[Close]

BLAST of CcUC07G144730 vs. NCBI nr
Match: XP_004146911.1 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_014948 [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 1.8e-89
Identity = 162/193 (83.94%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 16  LLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIEA 75
           LLDE GSIM ELSR+IMKLLL   GDN E+KFYESEFS CNGY+RINRYAPRNSN+EIEA
Sbjct: 127 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEA 186

Query: 76  FGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSA 135
           FGKHTDISCVTI+FQDEIGG+QMK K+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRSA
Sbjct: 187 FGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSA 246

Query: 136 EHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNYG 195
           EHRVVLKQ+VKRFSLAFF  F DDDRE+YAP EVVGEGNTR+YKPFSTKEYRA+RENNY 
Sbjct: 247 EHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 306

Query: 196 KIVGTPIREFAGV 206
           KIVG P+REFAG+
Sbjct: 307 KIVGVPLREFAGI 319

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BLAST of CcUC07G144730 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 282.0 bits (720), Expect = 4.3e-72
Identity = 137/200 (68.50%), Postives = 168/200 (84.00%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLL---LGDNFENKFYESEFSKCNGYIRINRYAPRNSNKEI 73
           SNLL+E GS MT+LS++I+KLL   LGDNFENKFY+ EF  C+GY+RIN Y+PR+SN+E+
Sbjct: 115 SNLLEEYGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEV 174

Query: 74  EAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLR 133
           E  GKHTDISCVTILFQD++GGLQM+SK   EW+D++P E+AL+VNIGDFMQAWSNERLR
Sbjct: 175 EGLGKHTDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLR 234

Query: 134 SAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFR--- 193
           SAEHRVVL+QNVKRFS+AFFW++ DD+ E+YAP EVVGEGN RLY+ FSTKEYR FR   
Sbjct: 235 SAEHRVVLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 294

Query: 194 -ENNYGKIVGTPIREFAGVL 207
               Y K VG  ++EFAG++
Sbjct: 295 ARGTYEK-VGANVKEFAGIV 311

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BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.6e-43
Identity = 97/213 (45.54%), Postives = 137/213 (64.32%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKE- 73
           S L+ E G  MT+L  KIMK +L   GD+  +K+YESEF  C+GY RIN Y   +  ++ 
Sbjct: 111 SGLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDD 170

Query: 74  ---------IEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDF 133
                    IE  G HTD+SC+TI+ QD+IGGLQ++++     +D+ P ++AL+VN+GD 
Sbjct: 171 HHNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDL 230

Query: 134 MQAWSNERLRSAEHRVVLKQN---VKRFSLAFFWTFGDDDREVYAPREVVG--EGNTRLY 193
           + AW+N RLRS++HRV+LK+      RFSLAFFW F DD + V+AP EVVG  EG  R++
Sbjct: 231 LHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVF 290

Query: 194 KPFSTKEYRAFRENN-YGKI--VGTPIREFAGV 206
           + F   +Y  FRE+N  GK   VG  + +FA +
Sbjct: 291 RSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

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BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 57/152 (37.50%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 39  NFENKFYESEFSKCNGYIRINRYAPRNSNKEIEAFGKHTDISCVTILFQDEIGGLQMKSK 98
           N  ++++E+ F   N ++R+N+Y P     E+     HTD S +TIL QD+IGGL++++ 
Sbjct: 179 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN- 238

Query: 99  RRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKRFSLAFFWTFGDDDR 158
              +WI VKP  +AL VNIGD  QA SN   +S  HRV+   N++R S+AFF        
Sbjct: 239 -NGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVC------ 298

Query: 159 EVYAPREVVGEGNTRLYKPFSTKEYRAFRENN 191
             Y   E+   G  + Y+ FS +EY+   E++
Sbjct: 299 -PYLETEIDCFGYPKKYRRFSFREYKEQSEHD 321

[Close]

BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 63/189 (33.33%), Postives = 98/189 (51.85%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLLLGDNFEN-KFYESEFSKCNGYIRINRYAPRNSNKEIEA 73
           S+ +++  S    L+  + ++L   + +N  F++    +   Y+R+NRY P     E+  
Sbjct: 152 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 211

Query: 74  FGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSA 133
              HTD   +TIL+QD++GGLQ+    R  WI VKP   AL++NIGD  QAWSN   +S 
Sbjct: 212 LMPHTDSDFLTILYQDQVGGLQLIKDNR--WIAVKPNPKALIINIGDLFQAWSNGMYKSV 271

Query: 134 EHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENN-- 193
           EHRV+    V+RFS A+F     D         +    +   Y+ FS +E+R   + +  
Sbjct: 272 EHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQVQEDVK 331

Query: 194 -YGKIVGTP 199
            +G  VG P
Sbjct: 332 KFGFKVGLP 331

[Close]

BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot
Match: G9M9M3 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-2-1 OS=Ipomoea batatas OX=4120 GN=F6H2-2-1 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.0e-20
Identity = 60/171 (35.09%), Postives = 96/171 (56.14%), Query Frame = 0


Query: 27  LSRKIMKLLLGDNFENKFYESEFSKCNGYIRIN-RYAPRNSNKEIE-AFGKHTDISCVTI 86
           +SRK+++ L+     N+  +S+ S   G  RIN  Y P+  N ++    G+H+DIS +T+
Sbjct: 180 VSRKLLEALMQGLNVNQIDDSKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTL 239

Query: 87  LFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKR 146
           L QD+IGGL ++    + W  V P++ AL++NIGD +Q  SN R +S EHRV+  ++  R
Sbjct: 240 LLQDDIGGLYVRKLEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVMANESNDR 299

Query: 147 FSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEY--RAFRENNYGK 194
            S+  F     +D     P EV+  G   +YKP    +Y    +R+ + GK
Sbjct: 300 ISVPVFVNPRPNDIVGPLP-EVLASGEKPVYKPVLYSDYAKHFYRKAHNGK 349

[Close]

BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot
Match: G9M9M5 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomoea batatas OX=4120 GN=F6H2-1-8 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.0e-20
Identity = 59/172 (34.30%), Postives = 96/172 (55.81%), Query Frame = 0


Query: 27  LSRKIMKLLLGDNFENKFYESEFSKCNGYIRIN-RYAPRNSNKEIE-AFGKHTDISCVTI 86
           +SRK+++ L+     N+  +S+ S   G  RIN  Y P+  N ++    G+H+DIS +T+
Sbjct: 181 VSRKLLEALMQGLNVNQIDDSKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTL 240

Query: 87  LFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKR 146
           L QD+IGGL ++    + W  V P++ AL++NIGD +Q  SN R +S EHRV+  ++  R
Sbjct: 241 LLQDDIGGLYVRKLEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVMANESNDR 300

Query: 147 FSLAFFWTFGDDDREVYAP-REVVGEGNTRLYKPFSTKEY--RAFRENNYGK 194
            S+  F        ++  P  EV+  G   +YKP    +Y    +R+ + GK
Sbjct: 301 ISVPVF--VNPKPNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGK 350

[Close]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL
Match: A0A5D3D2E7 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00730 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.4e-92
Identity = 165/194 (85.05%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 15  NLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIE 74
           NLLDE GSIM ELSR+IMKLLL   GD+FE+KFYESEFS CNGYIRINRYAPRNSN+EIE
Sbjct: 126 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE 185

Query: 75  AFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRS 134
           AFGKHTDISCVTILFQDEIGG+QMKSK+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRS
Sbjct: 186 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 245

Query: 135 AEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNY 194
           AEHRVVLKQN KRF+L FF  FGDDDRE+YAP EVVGEGN R+YKPFSTKEYRAFRENNY
Sbjct: 246 AEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 305

Query: 195 GKIVGTPIREFAGV 206
           GK+VG P+REFAG+
Sbjct: 306 GKVVGVPMREFAGI 319

[Close]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL
Match: A0A1S3BYJ4 (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.4e-92
Identity = 165/194 (85.05%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 15  NLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIE 74
           NLLDE GSIM ELSR+IMKLLL   GD+FE+KFYESEFS CNGYIRINRYAPRNSN+EIE
Sbjct: 126 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE 185

Query: 75  AFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRS 134
           AFGKHTDISCVTILFQDEIGG+QMKSK+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRS
Sbjct: 186 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 245

Query: 135 AEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNY 194
           AEHRVVLKQN KRF+L FF  FGDDDRE+YAP EVVGEGN R+YKPFSTKEYRAFRENNY
Sbjct: 246 AEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 305

Query: 195 GKIVGTPIREFAGV 206
           GK+VG P+REFAG+
Sbjct: 306 GKVVGVPMREFAGI 319

[Close]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL
Match: A0A5A7TME4 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004080 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.2e-91
Identity = 163/194 (84.02%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 15  NLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIE 74
           NLLDE GSIM ELSR+IMKLLL   GD+FE+KFYESEFS CNGYIRINRYAPRNSN+EIE
Sbjct: 126 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE 185

Query: 75  AFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRS 134
           AFGKHTDISCVTILFQDEIGG+QMKSK+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRS
Sbjct: 186 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 245

Query: 135 AEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNY 194
            EHRVVLKQN +RF+L FF  FGDDDRE+YAP EVVGEGN R+YKPFSTKEYRAFRENNY
Sbjct: 246 TEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 305

Query: 195 GKIVGTPIREFAGV 206
           GK+VG P+REFAG+
Sbjct: 306 GKVVGVPMREFAGI 319

[Close]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL
Match: A0A0A0KW97 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.5e-90
Identity = 162/193 (83.94%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 16  LLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKEIEA 75
           LLDE GSIM ELSR+IMKLLL   GDN E+KFYESEFS CNGY+RINRYAPRNSN+EIEA
Sbjct: 127 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEA 186

Query: 76  FGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSA 135
           FGKHTDISCVTI+FQDEIGG+QMK K+ DEW+DV+PLEDALLVNIGDF+QAWSNERLRSA
Sbjct: 187 FGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSA 246

Query: 136 EHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRENNYG 195
           EHRVVLKQ+VKRFSLAFF  F DDDRE+YAP EVVGEGNTR+YKPFSTKEYRA+RENNY 
Sbjct: 247 EHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 306

Query: 196 KIVGTPIREFAGV 206
           KIVG P+REFAG+
Sbjct: 307 KIVGVPLREFAGI 319

[Close]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.6e-72
Identity = 137/200 (68.50%), Postives = 168/200 (84.00%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLL---LGDNFENKFYESEFSKCNGYIRINRYAPRNSNKEI 73
           SNLL+E GS MT+LS++I+KLL   LGDNFENKFY+ EF  C+GY+RIN Y+PR+SN+E+
Sbjct: 115 SNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEV 174

Query: 74  EAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNERLR 133
           E  GKHTDISCVTILFQD++GGLQM+SK   EW+D++P E+AL+VNIGDFMQAWSNERLR
Sbjct: 175 EGLGKHTDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLR 234

Query: 134 SAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFR--- 193
           SAEHRVVL+QNVKRFS+AFFW++ DD+ E+YAP EVVGEGN RLY+ FSTKEYR FR   
Sbjct: 235 SAEHRVVLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 294

Query: 194 -ENNYGKIVGTPIREFAGVL 207
               Y K VG  ++EFAG++
Sbjct: 295 ARGTYEK-VGANVKEFAGIV 311

[Close]

BLAST of CcUC07G144730 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 3.8e-50
Identity = 97/204 (47.55%), Postives = 139/204 (68.14%), Query Frame = 0


Query: 17  LDECGSIMTELSRKIMKLL----LGDNFENKFYESEFSKCNGYIRINRYAP----RNSNK 76
           + E G+ M ELS++++K+L    LGD    + Y+++FS C+GY+R+  Y P        +
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 77  EIEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNER 136
            +E  G HTD+SC+TI++QD +GGLQM+SK   +WID+ P  D L+VNIGD MQAWSN R
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSK-EGKWIDINPCNDFLVVNIGDLMQAWSNGR 120

Query: 137 LRSAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRE 196
           LRS+EHRVVL++ V R SLAFF  F +D++ + AP+E+VGEG  R YK F   EY  FR+
Sbjct: 121 LRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 180

Query: 197 NN-YGKI--VGTPIREFAGVLESQ 210
           +N  GK   +G  +++FAG+  +Q
Sbjct: 181 SNEEGKFEKIGFTVKDFAGLKLAQ 202

[Close]

BLAST of CcUC07G144730 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 3.8e-50
Identity = 97/204 (47.55%), Postives = 139/204 (68.14%), Query Frame = 0


Query: 17  LDECGSIMTELSRKIMKLL----LGDNFENKFYESEFSKCNGYIRINRYAP----RNSNK 76
           + E G+ M ELS++++K+L    LGD    + Y+++FS C+GY+R+  Y P        +
Sbjct: 119 MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 178

Query: 77  EIEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDFMQAWSNER 136
            +E  G HTD+SC+TI++QD +GGLQM+SK   +WID+ P  D L+VNIGD MQAWSN R
Sbjct: 179 LVEGLGMHTDMSCITIVYQDSVGGLQMRSK-EGKWIDINPCNDFLVVNIGDLMQAWSNGR 238

Query: 137 LRSAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVGEGNTRLYKPFSTKEYRAFRE 196
           LRS+EHRVVL++ V R SLAFF  F +D++ + AP+E+VGEG  R YK F   EY  FR+
Sbjct: 239 LRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 298

Query: 197 NN-YGKI--VGTPIREFAGVLESQ 210
           +N  GK   +G  +++FAG+  +Q
Sbjct: 299 SNEEGKFEKIGFTVKDFAGLKLAQ 320

[Close]

BLAST of CcUC07G144730 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.8e-44
Identity = 97/213 (45.54%), Postives = 137/213 (64.32%), Query Frame = 0


Query: 14  SNLLDECGSIMTELSRKIMKLLL---GDNFENKFYESEFSKCNGYIRINRYAPRNSNKE- 73
           S L+ E G  MT+L  KIMK +L   GD+  +K+YESEF  C+GY RIN Y   +  ++ 
Sbjct: 111 SGLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDD 170

Query: 74  ---------IEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKPLEDALLVNIGDF 133
                    IE  G HTD+SC+TI+ QD+IGGLQ++++     +D+ P ++AL+VN+GD 
Sbjct: 171 HHNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDL 230

Query: 134 MQAWSNERLRSAEHRVVLKQN---VKRFSLAFFWTFGDDDREVYAPREVVG--EGNTRLY 193
           + AW+N RLRS++HRV+LK+      RFSLAFFW F DD + V+AP EVVG  EG  R++
Sbjct: 231 LHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVF 290

Query: 194 KPFSTKEYRAFRENN-YGKI--VGTPIREFAGV 206
           + F   +Y  FRE+N  GK   VG  + +FA +
Sbjct: 291 RSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of CcUC07G144730 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-23
Identity = 57/152 (37.50%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 39  NFENKFYESEFSKCNGYIRINRYAPRNSNKEIEAFGKHTDISCVTILFQDEIGGLQMKSK 98
           N  ++++E+ F   N ++R+N+Y P     E+     HTD S +TIL QD+IGGL++++ 
Sbjct: 179 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELEN- 238

Query: 99  RRDEWIDVKPLEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKRFSLAFFWTFGDDDR 158
              +WI VKP  +AL VNIGD  QA SN   +S  HRV+   N++R S+AFF        
Sbjct: 239 -NGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVC------ 298

Query: 159 EVYAPREVVGEGNTRLYKPFSTKEYRAFRENN 191
             Y   E+   G  + Y+ FS +EY+   E++
Sbjct: 299 -PYLETEIDCFGYPKKYRRFSFREYKEQSEHD 321

[Close]

BLAST of CcUC07G144730 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-23
Identity = 48/142 (33.80%), Postives = 83/142 (58.45%), Query Frame = 0


Query: 49  FSKCNGYIRINRYAPRNSNKEIEAFGKHTDISCVTILFQDEIGGLQMKSKRRDEWIDVKP 108
           F++   ++R N Y P  + +     G+H D   +T+L QD +GGLQ+  +   +WI VKP
Sbjct: 195 FNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKP 254

Query: 109 LEDALLVNIGDFMQAWSNERLRSAEHRVVLKQNVKRFSLAFFWTFGDDDREVYAPREVVG 168
           + DAL++N+G+ +Q W+N+   SAEHRVV+  + +RFS+ FF+ F   +  +    E++ 
Sbjct: 255 ISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFF-FPSHEANIEPLEELIS 314

Query: 169 EGNTRLYKPFSTKEYRAFRENN 191
           E N   YK ++  ++   R  +
Sbjct: 315 EENPPCYKKYNWGKFFVSRNRS 335

The following BLAST results are available for this feature:

BLAST of CcUC07G144730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876311.1	6.7e-97	91.75	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
XP_008453897.1	2.9e-92	85.05	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberell...	[more]
KAA0044653.1	2.4e-91	84.02	gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa]	[more]
XP_004146911.1	1.8e-89	83.94	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical p...	[more]
XP_022141254.1	4.3e-72	68.50	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]

BLAST of CcUC07G144730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.6e-43	45.54	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C6I4	1.9e-22	37.50	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
O49561	1.2e-21	33.33	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
G9M9M3	4.0e-20	35.09	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-2-1 OS=Ipomo...	[more]
G9M9M5	4.0e-20	34.30	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomo...	[more]

BLAST of CcUC07G144730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D2E7	1.4e-92	85.05	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BYJ4	1.4e-92	85.05	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
A0A5A7TME4	1.2e-91	84.02	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A0A0KW97	8.5e-90	83.94	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A6J1CI26	1.6e-72	68.50	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]

BLAST of CcUC07G144730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.2	3.8e-50	47.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.1	3.8e-50	47.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.8e-44	45.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G50960.1	1.4e-23	37.50	gibberellin 2-oxidase 7	[more]
AT3G19000.1	1.8e-23	33.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 76..97 score: 40.5 coord: 141..159 score: 36.84
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 13..196
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 13..196
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 15..191
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 57..150 e-value: 1.7E-21 score: 76.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..186 e-value: 3.1E-47 score: 163.7
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 52..153 score: 11.819923

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC07G144730.1	CcUC07G144730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding