CcUC07G142150 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC07G142150
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	E3 ubiquitin-protein ligase RNF144B-like
Location	CicolChr07: 28318444 .. 28318896 (+)
RNA-Seq Expression	CcUC07G142150
Synteny	CcUC07G142150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCGAAATCCGCCTCCGAAATGTTCACAAACAGAGTCTGTTCCCATACCTTCTGCACCGATTGCATCTCAAGGCACATCGCCGCAAAGCTTGAGGAGACAATGGCGGTGAAGTGCCCGGAGCCGGAAATGTGCGGAGGTTTGGTTCCGAAAGAGGTGTTGGAGCGTTGGGGGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAAGTGTCCTTTCAAAGACTGCTCGGCGCCGATGATCGACGAGGGGGATGAGGAGGCGGTGGAGTGTCCGAGTTGTTGGAGAATGTTCTGCACTCAGTGTAAAGTGGGATGGCATGGGGAAATGGAATGCGGAGAATTTGAGAAACTGCGGAAGGAGGCAGGAGGAAGTGTAGACAGAGACGATGGGATGACGATGAAACTGGCGGATAAAAGCAAAATGGAAGAGATGCCCTAA

mRNA sequence

ATGGATGCGAAATCCGCCTCCGAAATGTTCACAAACAGAGTCTGTTCCCATACCTTCTGCACCGATTGCATCTCAAGGCACATCGCCGCAAAGCTTGAGGAGACAATGGCGGTGAAGTGCCCGGAGCCGGAAATGTGCGGAGGTTTGGTTCCGAAAGAGGTGTTGGAGCGTTGGGGGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAAGTGTCCTTTCAAAGACTGCTCGGCGCCGATGATCGACGAGGGGGATGAGGAGGCGGTGGAGTGTCCGAGTTGTTGGAGAATGTTCTGCACTCAGTGTAAAGTGGGATGGCATGGGGAAATGGAATGCGGAGAATTTGAGAAACTGCGGAAGGAGGCAGGAGGAAGTGTAGACAGAGACGATGGGATGACGATGAAACTGGCGGATAAAAGCAAAATGGAAGAGATGCCCTAA

Coding sequence (CDS)

ATGGATGCGAAATCCGCCTCCGAAATGTTCACAAACAGAGTCTGTTCCCATACCTTCTGCACCGATTGCATCTCAAGGCACATCGCCGCAAAGCTTGAGGAGACAATGGCGGTGAAGTGCCCGGAGCCGGAAATGTGCGGAGGTTTGGTTCCGAAAGAGGTGTTGGAGCGTTGGGGGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAAGTGTCCTTTCAAAGACTGCTCGGCGCCGATGATCGACGAGGGGGATGAGGAGGCGGTGGAGTGTCCGAGTTGTTGGAGAATGTTCTGCACTCAGTGTAAAGTGGGATGGCATGGGGAAATGGAATGCGGAGAATTTGAGAAACTGCGGAAGGAGGCAGGAGGAAGTGTAGACAGAGACGATGGGATGACGATGAAACTGGCGGATAAAAGCAAAATGGAAGAGATGCCCTAA

Protein sequence

MDAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCPEPEMCGGLVPKEVLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP

Homology

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BLAST of CcUC07G142150 vs. NCBI nr
Match: XP_008460341.1 (PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa] >TYK19145.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 248.1 bits (632), Expect = 5.0e-62
Identity = 117/160 (73.12%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAKS S+MFTNRVC+HTFCTDCIS+HIAAKLE TMAVKCP        EPEMCG  VPK 
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERW DALFEAMILKSKRL CPFKDC A MIDEGDEE   AVEC SCWR+FC +C+VGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HGEM+CGEF++LRKEAG S D+DD MT+KLA+  K    P
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCP 220

[Close]

BLAST of CcUC07G142150 vs. NCBI nr
Match: XP_004144476.2 (probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypothetical protein Csa_001774 [Cucumis sativus])

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-58
Identity = 113/160 (70.62%), Postives = 124/160 (77.50%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAKS S+MFTNRVC+HTFCT CIS HIAAKLE  MAVKCP        EPEMCG  VPK 
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERW DALFEAMILK KRL CPFKDC A +IDEG EE   AVEC SCWR+FC +C+VGW
Sbjct: 121 VLERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGW 180

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HGEMECGEF++LRKEAG S D+DD MT+KLA+  K    P
Sbjct: 181 HGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCP 220

[Close]

BLAST of CcUC07G142150 vs. NCBI nr
Match: XP_038889081.1 (probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida])

HSP 1 Score: 230.3 bits (586), Expect = 1.1e-56
Identity = 112/160 (70.00%), Postives = 128/160 (80.00%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAK ASEMFTN +CSHTFCTDCIS++IAAKLE++MAVKCP        EPEMC   +PKE
Sbjct: 66  DAKLASEMFTNGLCSHTFCTDCISKYIAAKLEDSMAVKCPQPKCGTLLEPEMCRSFIPKE 125

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERWGDALFEAMILK KRL CPFKDCSAPMI EGDE+   AVEC +CWR+FC QCKVGW
Sbjct: 126 VLERWGDALFEAMILKWKRLNCPFKDCSAPMIYEGDEKGVTAVECLNCWRLFCAQCKVGW 185

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HG+MEC EFEK+RKE     + +D MTMKLAD+ K ++ P
Sbjct: 186 HGDMECREFEKVRKE-----NTEDMMTMKLADEKKWKKCP 220

[Close]

BLAST of CcUC07G142150 vs. NCBI nr
Match: KAG6581788.1 (putative E3 ubiquitin-protein ligase ARI5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 221.1 bits (562), Expect = 6.5e-54
Identity = 104/157 (66.24%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           D KSASEMFT+  CSHTFC DCIS+HIAAKLE++MAVKCP        EPEMC   VPKE
Sbjct: 79  DTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKE 138

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGE 121
           VLERWGDAL +AM+LK K + CPFKDCSA M+DE D EAVECP CWRMFC +C VGWHG+
Sbjct: 139 VLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDMEAVECPECWRMFCAKCGVGWHGD 198

Query: 122 MECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           M C EF++L K   G VDR++ MTMKLAD+ K +  P
Sbjct: 199 MNCTEFQRLLK--NGGVDREEEMTMKLADERKWKRCP 233

[Close]

BLAST of CcUC07G142150 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 221.1 bits (562), Expect = 6.5e-54
Identity = 104/157 (66.24%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           D KSASEMFT+  CSHTFC DCIS+HIAAKLE++MAVKCP        EPEMC   VPKE
Sbjct: 79  DTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKE 138

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGE 121
           VLERWGDAL +AM+LK K + CPFKDCSA M+DE D EAVECP CWRMFC +C VGWHG+
Sbjct: 139 VLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDMEAVECPKCWRMFCAKCGVGWHGD 198

Query: 122 MECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           M C EF++L K   G VDR++ MTMKLAD+ K +  P
Sbjct: 199 MNCTEFQRLLK--NGGVDREEEMTMKLADERKWKRCP 233

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BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot
Match: Q7Z419 (E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.6e-09
Identity = 38/136 (27.94%), Postives = 57/136 (41.91%), Query Frame = 0


Query: 4   KSASEMFTNRVCSHTFCTDCISRHIAAKLEETMA--VKCP----------EPEMCGGLVP 63
           +S  +M T + C   FCT C+ +++   + E     + CP          +      LVP
Sbjct: 37  QSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVP 96

Query: 64  KEVLERWGDALFEAMI-LKSKRLKCPFKDCS----APMIDEGDEEAVECPSCWRMFCTQC 123
            +  + +    FE  + L   R  CP  DC         D G    VECPSC   FC+ C
Sbjct: 97  VDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCC 156

[Close]

BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.5e-08
Identity = 38/130 (29.23%), Postives = 54/130 (41.54%), Query Frame = 0


Query: 4   KSASEMFTNRVCSHTFCTDCISRHIAAKLEETMA--VKCP----------EPEMCGGLVP 63
           +S  +M T + C   FCT C+ +++   + E     + CP          +      LVP
Sbjct: 38  QSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVP 97

Query: 64  KEVLERWGDALFEAMI-LKSKRLKCPFKDCS----APMIDEGDEEAVECPSCWRMFCTQC 117
            +  + +    FE  + L   R  CP  DC         D G    VECPSC   FC+ C
Sbjct: 98  VDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVATSDPGQPVLVECPSCHLKFCSCC 157

[Close]

BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot
Match: Q8BKD6 (E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 5.6e-08
Identity = 40/153 (26.14%), Postives = 59/153 (38.56%), Query Frame = 0


Query: 4   KSASEMFTNRVCSHTFCTDCISRHIAAKLEETMA--VKCP----------EPEMCGGLVP 63
           +S  +M   + C   FCT C+ +++   + E     + CP          +      LVP
Sbjct: 37  QSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCLNHGTLQETEIACLVP 96

Query: 64  KEVLERWGDALFEAMI-LKSKRLKCPFKDCSA----PMIDEGDEEAVECPSCWRMFCTQC 123
            +  + +    FE  + +   R  CP  DC         D G    VECPSC   FC+ C
Sbjct: 97  LDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPSCHLKFCSCC 156

Query: 124 KVGWHGEMECGEFEKLRKEAGGSVDRDDGMTMK 140
           K  WH E  C + +    E G     D    +K
Sbjct: 157 KDAWHEESSCRDSQSAMPEHGALFGTDADAPIK 189

[Close]

BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot
Match: Q5RFV4 (Probable E3 ubiquitin-protein ligase RNF144A-A OS=Danio rerio OX=7955 GN=rnf144aa PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.2e-07
Identity = 35/128 (27.34%), Postives = 56/128 (43.75%), Query Frame = 0


Query: 8   EMFTNRVCSHTFCTDCISRHIAAKLEE--TMAVKCP----------EPEMCGGLVPKEVL 67
           +M T   C   FCT C+ +++   ++E    A+ CP          +      +V  E++
Sbjct: 31  QMTTITQCQCVFCTMCLKQYVELLIKEGFETAISCPDSACPKRGHLQENEIECMVATEIM 90

Query: 68  ERWGDALFE-AMILKSKRLKCPFKDCSAP-MIDEGD---EEAVECPSCWRMFCTQCKVGW 119
           +R+    FE  ++L   R  CP   C A   + E D    + V C  C   FC+ CK  W
Sbjct: 91  QRYRKLQFEKEVLLDPSRTWCPSSTCQAVCQLKESDTVLPQLVRCSVCTLEFCSACKASW 150

[Close]

BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot
Match: Q9JI90 (E3 ubiquitin-protein ligase RNF14 OS=Mus musculus OX=10090 GN=Rnf14 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 6.2e-07
Identity = 30/113 (26.55%), Postives = 52/113 (46.02%), Query Frame = 0


Query: 15  CSHTFCTDCISRHIAAKLE--ETMAVKCPEPEMCGGLVP---KEVLERWGDALFEAMILK 74
           C H +C  C+  +   +++  +   + CPEP+      P   KE++E    A ++ ++L+
Sbjct: 239 CKHVYCKACLKDYFEIQIKDGQVKCLNCPEPQCPSVATPGQVKELVEADLFARYDRLLLQ 298

Query: 75  SKR------LKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGEMEC 117
           S        + CP   C  P++ E       C SC   FCT C++ +HG   C
Sbjct: 299 STLDLMADVVYCPRPCCQLPVMQEPGGTMAICSSCNFAFCTLCRLTYHGLSPC 351

[Close]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL
Match: A0A5A7UED3 (E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00700 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.4e-62
Identity = 117/160 (73.12%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAKS S+MFTNRVC+HTFCTDCIS+HIAAKLE TMAVKCP        EPEMCG  VPK 
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERW DALFEAMILKSKRL CPFKDC A MIDEGDEE   AVEC SCWR+FC +C+VGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HGEM+CGEF++LRKEAG S D+DD MT+KLA+  K    P
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCP 220

[Close]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL
Match: A0A1S3CDI0 (E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.4e-62
Identity = 117/160 (73.12%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAKS S+MFTNRVC+HTFCTDCIS+HIAAKLE TMAVKCP        EPEMCG  VPK 
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERW DALFEAMILKSKRL CPFKDC A MIDEGDEE   AVEC SCWR+FC +C+VGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HGEM+CGEF++LRKEAG S D+DD MT+KLA+  K    P
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCP 220

[Close]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL
Match: A0A0A0L952 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 5.5e-59
Identity = 113/160 (70.62%), Postives = 124/160 (77.50%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           DAKS S+MFTNRVC+HTFCT CIS HIAAKLE  MAVKCP        EPEMCG  VPK 
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEE---AVECPSCWRMFCTQCKVGW 121
           VLERW DALFEAMILK KRL CPFKDC A +IDEG EE   AVEC SCWR+FC +C+VGW
Sbjct: 121 VLERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGW 180

Query: 122 HGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           HGEMECGEF++LRKEAG S D+DD MT+KLA+  K    P
Sbjct: 181 HGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCP 220

[Close]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.1e-54
Identity = 104/157 (66.24%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCP--------EPEMCGGLVPKE 61
           D KSASEMFT+  CSHTFC DCIS+HIAAKLE++MAVKCP        EPEMC   VPKE
Sbjct: 79  DTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKE 138

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGE 121
           VLERWGDAL +AM+LK K + CPFKDCSA M+DE D EAVECP CWRMFC +C VGWHG+
Sbjct: 139 VLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDMEAVECPKCWRMFCAKCGVGWHGD 198

Query: 122 MECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           M C EF++L K   G VDR++ MTMKLAD+ K +  P
Sbjct: 199 MNCTEFQRLLK--NGGVDREEEMTMKLADERKWKRCP 233

[Close]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL
Match: A0A6J1IU95 (probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC111479983 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.3e-52
Identity = 101/157 (64.33%), Postives = 118/157 (75.16%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCPE--------PEMCGGLVPKE 61
           D KSASEMFT+  CSHTFC DCIS+HIAAKL +++AVKCPE        PEMC   VPKE
Sbjct: 78  DTKSASEMFTSTGCSHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKE 137

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVECPSCWRMFCTQCKVGWHGE 121
           VLERWGDAL +AM+LK K + CPFKDCSA M+DE D EAVECP CWRMFC +C VGWH +
Sbjct: 138 VLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDREAVECPKCWRMFCAKCGVGWHAD 197

Query: 122 MECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           M C EF++L K   G VDR++ MTMKLAD+ K +  P
Sbjct: 198 MNCTEFQRLLK--NGGVDREEEMTMKLADERKWKRCP 232

[Close]

BLAST of CcUC07G142150 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 2.3e-33
Identity = 69/162 (42.59%), Postives = 99/162 (61.11%), Query Frame = 0


Query: 1   MDAKSASEMFTNRVCSHTFCTDCISRHIAAKLEET-MAVKCP--------EPEMCGGLVP 60
           +D+KS  E F    CSH +C DC+S++IAAKL++  ++++CP        EP+ C  ++P
Sbjct: 119 VDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGRLEPDQCRQILP 178

Query: 61  KEVLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAV---ECPSCWRMFCTQCKV 120
           KEV +RWGDAL EA++++SK+  CP+KDCSA +  E  E  +   ECP C RM C +C  
Sbjct: 179 KEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRMVCVECGT 238

Query: 121 GWHGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
            WH EM C EF+KL     G   RDD +   +A + K +  P
Sbjct: 239 QWHPEMTCEEFQKLAANERG---RDDILLATMAKQKKWKRCP 277

[Close]

BLAST of CcUC07G142150 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 9.1e-30
Identity = 62/164 (37.80%), Postives = 92/164 (56.10%), Query Frame = 0


Query: 1   MDAKSASEMFTNRV-CSHTFCTDCISRHIAAKLEETMA-VKCP--------EPEMCGGLV 60
           MD K +S++F     C+H +CTDC  R++A K++E  + +KCP        EP  C  L+
Sbjct: 99  MDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTCRDLI 158

Query: 61  PKEVLERWGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEA----VECPSCWRMFCTQC 120
           PK+V +RW   L E++I    +  CPFKDCSA M++  + +A     EC SC R+FC QC
Sbjct: 159 PKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLFCVQC 218

Query: 121 KVGWHGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEEMP 151
           KV WH  + C EF++       S D DD + +++A   +    P
Sbjct: 219 KVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCP 262

[Close]

BLAST of CcUC07G142150 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 87.4 bits (215), Expect = 1.0e-17
Identity = 46/141 (32.62%), Postives = 65/141 (46.10%), Query Frame = 0


Query: 6   ASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCPEP--------EMCGGLVPKEVLER 65
           A  MF+   C H FC  C+ +H+  KL   MA KCP          + CG L+  ++ + 
Sbjct: 310 AEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSELVIDACGKLLTPKLSKL 369

Query: 66  WGDALFEAMILKSKRLKCPFKDCSAPMIDEGDEEAVE-------------CPSCWRMFCT 125
           W   L E  I  ++R+ CP+  CSA M      E+ +             C  C  +FC 
Sbjct: 370 WQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCV 429

[Close]

BLAST of CcUC07G142150 vs. TAIR 10
Match: AT3G45540.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 83.6 bits (205), Expect = 1.5e-16
Identity = 47/162 (29.01%), Postives = 76/162 (46.91%), Query Frame = 0


Query: 2   DAKSASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCPEPEM--------CGGLVPKE 61
           D  + ++MF    C H FC+DC+ RHI  +L E   ++CP            C  L+  +
Sbjct: 106 DDINENQMFCVGKCRHRFCSDCMRRHIEVRLLEGSVMRCPHYRCKTTLKFGGCINLLTPK 165

Query: 62  VLERWGDALFEAMILKSKRLKCPFKDCSAPMID-------EGDEEAVECPSCWRMFCTQC 121
           + E W   + E +I  + R+ CP   CSA M +       + DE    C  C ++FC +C
Sbjct: 166 IREMWQQRIKEDLIPVTGRIYCPNSRCSALMSETELSISTKEDEVRRCCFKCGQIFCIKC 225

Query: 122 KVGWHGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEE 149
           KV WH  + C +++KL          +DG    LA++ +  +
Sbjct: 226 KVSWHSNLSCNDYKKLNPYP----TENDGKIKALANQKRWRQ 263

[Close]

BLAST of CcUC07G142150 vs. TAIR 10
Match: AT3G43750.1 (RING/U-box protein with C6HC-type zinc finger domain )

HSP 1 Score: 78.2 bits (191), Expect = 6.3e-15
Identity = 45/159 (28.30%), Postives = 74/159 (46.54%), Query Frame = 0


Query: 6   ASEMFTNRVCSHTFCTDCISRHIAAKLEETMAVKCPEPE--------MCGGLVPKEVLER 65
           A +MF+  +C H FC +C+  +I  KL E    +C + +         CG L+  ++   
Sbjct: 165 AEKMFSVAICGHQFCVECVKHYIEVKLLEGGVPRCLDYQCESKLTLTSCGNLLTPKLKAI 224

Query: 66  WGDALFEAMILKSKRLKCPFKDCSAPM----IDEGDEEAVE----CPSCWRMFCTQCKVG 125
           W   + E +IL ++R+ CP   CS  M    +    EE V     C  C   FC  CKV 
Sbjct: 225 WKQRIEEELILVAERVYCPNPRCSGLMSKTELSTSTEEDVSTRTCCVKCGEPFCINCKVP 284

Query: 126 WHGEMECGEFEKLRKEAGGSVDRDDGMTMKLADKSKMEE 149
           WH  + C ++++L    G +  ++D     LA++ K  +
Sbjct: 285 WHSNLSCDDYKRL----GPNPTKNDIKLKVLANQQKWSQ 319

The following BLAST results are available for this feature:

BLAST of CcUC07G142150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008460341.1	5.0e-62	73.13	PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1...	[more]
XP_004144476.2	1.1e-58	70.63	probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypot...	[more]
XP_038889081.1	1.1e-56	70.00	probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida]	[more]
KAG6581788.1	6.5e-54	66.24	putative E3 ubiquitin-protein ligase ARI5, partial [Cucurbita argyrosperma subsp...	[more]
XP_022956145.1	6.5e-54	66.24	probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata]	[more]

BLAST of CcUC07G142150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Z419	8.6e-09	27.94	E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1	[more]
A5PK27	2.5e-08	29.23	E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1	[more]
Q8BKD6	5.6e-08	26.14	E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=...	[more]
Q5RFV4	1.2e-07	27.34	Probable E3 ubiquitin-protein ligase RNF144A-A OS=Danio rerio OX=7955 GN=rnf144a...	[more]
Q9JI90	6.2e-07	26.55	E3 ubiquitin-protein ligase RNF14 OS=Mus musculus OX=10090 GN=Rnf14 PE=1 SV=2	[more]

BLAST of CcUC07G142150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UED3	2.4e-62	73.13	E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CDI0	2.4e-62	73.13	E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194...	[more]
A0A0A0L952	5.5e-59	70.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1	[more]
A0A6J1GX07	3.1e-54	66.24	probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IU95	1.3e-52	64.33	probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CcUC07G142150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	2.3e-33	42.59	RING/U-box superfamily protein	[more]
AT3G14250.1	9.1e-30	37.80	RING/U-box superfamily protein	[more]
AT5G60250.1	1.0e-17	32.62	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G45540.1	1.5e-16	29.01	RING/U-box protein with C6HC-type zinc finger	[more]
AT3G43750.1	6.3e-15	28.30	RING/U-box protein with C6HC-type zinc finger domain	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 56..116 e-value: 8.6E-14 score: 61.8
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 58..116 e-value: 3.8E-12 score: 46.2
None	No IPR available	GENE3D	2.20.25.20		coord: 73..121 e-value: 5.2E-6 score: 28.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..150
None	No IPR available	PANTHER	PTHR11685:SF309	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 2..147
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 4..65
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 59..121
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..67 e-value: 6.8E-12 score: 47.2
IPR027370	RING-type zinc-finger, LisH dimerisation motif	PFAM	PF13445	zf-RING_UBOX	coord: 10..41 e-value: 9.7E-6 score: 25.5
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 2..147
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 15..24
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 1..150 score: 16.281162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC07G142150.1	CcUC07G142150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:1901363	heterocyclic compound binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0097159	organic cyclic compound binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity