CcUC04G065860 (gene) Watermelon (PI 537277) v1

BLAST of CcUC04G065860 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.4e-310
Identity = 532/577 (92.20%), Postives = 553/577 (95.84%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           M+N + HSS DAPRKK   GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA
Sbjct: 1   MDNENRHSSSDAPRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLL GSIASF+GMGVL+LGAGLPQMRPPP
Sbjct: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180
           CI+GE++CPQP PWQL FLYLGLGLIV+GAGGIRPCNISFGADQFDTNTEKGRAKLESFL
Sbjct: 121 CISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180

Query: 181 NWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGS 240
           NWWY LFSIALV+ALTIVVYVQTNISWTLGFAIPTACFV SILIFLSG +FYVCKAPQGS
Sbjct: 181 NWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGS 240

Query: 241 VFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELD 300
           VF+D+AKVVVA  RK  +PKP QIDHLHNPPMNSCKLAHTDRFLVFDKAATV+DSS+ELD
Sbjct: 241 VFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRFLVFDKAATVVDSSIELD 300

Query: 301 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 360
           EEGKSK+EWRLCSVHQVEQFKC+VGIIPVWL GI CFISMQQMGSFGILQAIQMNRFIGP
Sbjct: 301 EEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGP 360

Query: 361 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVV 420
           HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKT +NKR SMKTRIEIGIVMSVLCMVV
Sbjct: 361 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVV 420

Query: 421 AGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480
           AG+LEKLRRDAAVENKSFVS LHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV
Sbjct: 421 AGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480

Query: 481 AGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAAL 540
           AGAIFFLSLSLASYLSSVLMGIVSKIGRME GE+QWLGGNDLNKNRL+YFFYVVGGIAAL
Sbjct: 481 AGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAAL 540

Query: 541 NFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           NFFYFRFFAAGFLP PDVDPRQKAHQRQLEDAELGRR
Sbjct: 541 NFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 577

BLAST of CcUC04G065860 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.5e-292
Identity = 507/578 (87.72%), Postives = 532/578 (92.04%), Query Frame = 0

Query: 1   MENGS-LHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           MENG+ LH+SLDA R K T GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLL GS+AS +GMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCI+GE+NCPQP PWQL FLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQG 240
           LNWWYFLFSI+LV+ALTIVVYVQTNISWTLGF IPTACFVFSI IFL G ++Y+CKAPQG
Sbjct: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240

Query: 241 SVFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMEL 300
           SVFADMAKVVVATCRK  +PK TQ D LHNPPMNS KLAHT+RFL+FDKAATV+DSS+EL
Sbjct: 241 SVFADMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIG 360
           DEEGKSKNEWRLCSVHQVEQFKC+VGI PVWL GIPCF+SMQQM SFGILQAIQMNR IG
Sbjct: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT++NKR SMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480
           VAG+LEK RRDAAVENKSF+S LHVW+LIPEFALSGLTEAF AIAVMELLTTHLPESLRT
Sbjct: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAA 540
           VAGAIFF SLSLASYLSSVL GIV       G E +WLGGNDLNKNRL+YFF VVG IAA
Sbjct: 481 VAGAIFFFSLSLASYLSSVLTGIV------RGVEREWLGGNDLNKNRLDYFFSVVGVIAA 540

Query: 541 LNFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           LNFFYFRFFA  FLPKPDVDP  KAHQ QLEDAELGRR
Sbjct: 541 LNFFYFRFFAVAFLPKPDVDPSHKAHQMQLEDAELGRR 572

BLAST of CcUC04G065860 vs. NCBI nr
Match: XP_023544027.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972.6 bits (2513), Expect = 1.5e-279
Identity = 480/558 (86.02%), Postives = 514/558 (92.11%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           ME+G LHSS + P  KA  GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V 
Sbjct: 1   MESGRLHSSFNRP--KAALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSVD 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLL GS AS +GMGVLTLGAG P+MRPPP
Sbjct: 61  SAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180
           C  GE+NCPQPHPWQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL
Sbjct: 121 CRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180

Query: 181 NWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGS 240
           NWWY LFS+ALV+ALT+VVYVQT+ISWTLGFAIPTACFVFSILIFL G +FY+CK PQGS
Sbjct: 181 NWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGS 240

Query: 241 VFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELD 300
           VFADMAKV+VATCRK  VPK TQIDHLH+PPM+S KL+HTDRFLVFDKAATV+DSS ELD
Sbjct: 241 VFADMAKVLVATCRKRNVPKFTQIDHLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTELD 300

Query: 301 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 360
           E+GKSKNEW+LCSVHQVEQFKC+VGI+P+W+ GIPCFISMQQMGSFGILQAIQMNRFIGP
Sbjct: 301 EQGKSKNEWKLCSVHQVEQFKCVVGIVPIWVAGIPCFISMQQMGSFGILQAIQMNRFIGP 360

Query: 361 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVV 420
           HFQIPPAWMSLTPMIALSIWIYIYEK+VEFMKKK   NKR SMKTRIEIGIVMSV+CMVV
Sbjct: 361 HFQIPPAWMSLTPMIALSIWIYIYEKFVEFMKKKNHKNKRFSMKTRIEIGIVMSVICMVV 420

Query: 421 AGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480
           AGILE +RR++AVENKSFVS LHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV
Sbjct: 421 AGILENVRRNSAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480

Query: 481 AGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAAL 540
           AGAIFFLSLSL SY+SSVLM IVSKI R+EGG+  WLGGNDLNKNRL+YFFYVVG IAA 
Sbjct: 481 AGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAAF 540

Query: 541 NFFYFRFFAAGFLPKPDV 559
           NF YFRFFA  FLPKP+V
Sbjct: 541 NFLYFRFFATHFLPKPNV 556

BLAST of CcUC04G065860 vs. NCBI nr
Match: KAG6603629.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 967.6 bits (2500), Expect = 4.7e-278
Identity = 481/559 (86.05%), Postives = 512/559 (91.59%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           ME+G LHSS++ P  K   GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V 
Sbjct: 1   MESGRLHSSVNRP--KIALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSVD 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLL GS AS +GMGVLTLGAG P+MRPPP
Sbjct: 61  SAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180
           C  GE+NCPQPHPWQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL
Sbjct: 121 CRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180

Query: 181 NWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGS 240
           NWWY LFS+ALV+ALT+VVYVQT+ISWTLGFAIPTACFVFSILIFL G +FY+CK PQGS
Sbjct: 181 NWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGS 240

Query: 241 VFADMAKVVVATCRKHGVPKPTQIDH-LHNPPMNSCKLAHTDRFLVFDKAATVIDSSMEL 300
           VFADMAKV+VATCRK  VPK TQIDH LH+PPM+S KL+HTDRFLVFDKAATV+DSS EL
Sbjct: 241 VFADMAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIG 360
           DE+GKSKNEW+LCSVHQVEQFKC VGI+P+W  GIPCFISMQQMGSFGILQAIQMNRFIG
Sbjct: 301 DEQGKSKNEWKLCSVHQVEQFKCFVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKT  NKR S+KTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSLKTRIEIGIVMSVLCMV 420

Query: 421 VAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480
           VAGILE +RR +A ENKSFVS LHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT
Sbjct: 421 VAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAA 540
           VAGAIFFLSLSL SY+SSVLM IVSKI R+EGG+  WLGGNDLNKNRL+YFFYVVG IAA
Sbjct: 481 VAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAAGFLPKPDV 559
            NFFYFRFFA  FLPKP+V
Sbjct: 541 FNFFYFRFFATHFLPKPNV 557

BLAST of CcUC04G065860 vs. NCBI nr
Match: KAG6595347.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027355.1 Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 923.7 bits (2386), Expect = 7.8e-265
Identity = 457/577 (79.20%), Postives = 514/577 (89.08%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           M+NG+L SSLD P  K   GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV 
Sbjct: 1   MDNGTLRSSLD-PSPKPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNVD 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLL GSIASF+GMGVLTLGA LP+MRPPP
Sbjct: 61  SAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180
           C +G+T+C Q   WQL+FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL
Sbjct: 121 CSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180

Query: 181 NWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGS 240
           NWWYFLFSIALV+ALT+VVY+QTNISWTLGFAIPTA F+FSI+IFL G   Y+C+ PQGS
Sbjct: 181 NWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQGS 240

Query: 241 VFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELD 300
           VFADMAKV++AT RK  +P+P+  +HLH+PPM+S KL HT+RFLVFDKAA VIDSSMELD
Sbjct: 241 VFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMELD 300

Query: 301 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 360
           E+G+SKNEW+LC+VHQVEQ KC+VGI+P+W  GI CFISMQQMGSFGILQAIQMNRF+GP
Sbjct: 301 EQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLGP 360

Query: 361 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVV 420
           HF+IPPAWMSL PMIALS+WIYIYEKYVEFM++KT +NKR SMK RIEIGIVMSVLCM+V
Sbjct: 361 HFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMIV 420

Query: 421 AGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480
           AGILEK+RRD+A+EN++FVS LHV +LIPEFALSGLTEAFAAI+VMELLTT LPESLRTV
Sbjct: 421 AGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRTV 480

Query: 481 AGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAAL 540
           AGAIFFLSLSLASYLSSVLMGIV+++ +    +  WL GNDLNKN L+YFFYVVG IAAL
Sbjct: 481 AGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAAL 540

Query: 541 NFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           NFFYFRF    FLP+ DVDPR KA +R+ ED E G R
Sbjct: 541 NFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of CcUC04G065860 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 572.0 bits (1473), Expect = 7.6e-162
Identity = 294/544 (54.04%), Postives = 382/544 (70.22%), Query Frame = 0

Query: 16  KATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 75
           K   GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 76  PLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGETNCPQPHPWQ 135
            LAGAF++DAYLGRF+TLLLGSIASF+GMG+  L A LP +RP  CI       QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 136 LSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALVVAL 195
           L  L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F++ALV+AL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 196 TIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVATCRK 255
           T VVY+QTNISW +GF IPTAC   SI  F+ G   Y+C   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 256 HGVPKPTQIDHLHNP-----PMNSCKLAHTDRFLVFDKAATVIDSSMELDEEGKSKNEWR 315
             V   + I     P     P    +  H  RF  FDK A+++ +  EL+E+G +K +WR
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRF--FDK-ASIVTNPNELNEDGNAKYKWR 314

Query: 316 LCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGPH-FQIPPAWM 375
           LCSV QV+  KC+  I+PVW+TGI CFI   Q   +GILQA+QM++  GPH FQ+P  WM
Sbjct: 315 LCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWM 374

Query: 376 SLTPMIALSIWIYIYE-KYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGILEKLR 435
           +L  MI L+IWI +YE   +  +K+ T   KRL++K RIE  IVM ++CM+VAG  EK R
Sbjct: 375 NLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKR 434

Query: 436 RDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLS 495
           R +A++N SFVS + + ML+P+FAL+GLTEAF+A+A+ME LT  +PE +R VAGAIFFLS
Sbjct: 435 RASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLS 494

Query: 496 LSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAALNFFYFRFF 553
            S+ASY+ ++L+ ++  + R EG    WLG  DLNKNRLE +F+++ GI   N  YFR F
Sbjct: 495 SSIASYICTLLINVIDAVTRKEGK--SWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLF 551

BLAST of CcUC04G065860 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 5.9e-114
Identity = 218/579 (37.65%), Postives = 356/579 (61.49%), Query Frame = 0

Query: 8   SSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 67
           S LDA + +  PGGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  I
Sbjct: 41  SILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINI 100

Query: 68  WGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGE-T 127
           W G TN  PL GA+++D Y+GRF T+   S A+ +G+  +TL A  PQ+ P  C + +  
Sbjct: 101 WSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL 160

Query: 128 NCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 187
           +C  P+  Q+  L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  
Sbjct: 161 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 220

Query: 188 FSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMA 247
           F++ L++  T+VVY+Q  +SW +GF+IPT     ++++F +G + YV   P+GS+F+ +A
Sbjct: 221 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 280

Query: 248 KVVVATCRKHGVPKPTQID---HLHNPPMNS---CKLAHTDRFLVFDKAATVIDSSMELD 307
           +V+VA  +K  +  P + D     ++P + S    KL  +++F   DKAA VI+   +L 
Sbjct: 281 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLT 340

Query: 308 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 367
            EG   ++WRLCSV +VE+ KC++ I+P+W  GI    +M   G+F + QA++M+R +GP
Sbjct: 341 PEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGP 400

Query: 368 HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMV 427
            F+IP   +S+  ++ + I++  Y++ +V FM++ T +   +++  RI  GIV ++  M+
Sbjct: 401 KFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMI 460

Query: 428 VAGILEKLRRDAAVE--NKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESL 487
           VAGI+E++RR  ++   + + ++ + V+ L P+  L GL EAF  I  +E   +  PE +
Sbjct: 461 VAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHM 520

Query: 488 RTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQ--WLGGNDLNKNRLEYFFYVVG 547
           R++A ++F LS + +SYLSS L+ +V K     GG  +  WL  N LN  +L+YF+Y++ 
Sbjct: 521 RSIANSLFSLSFAGSSYLSSFLVTVVHK---FSGGHDRPDWLNKN-LNAGKLDYFYYLIA 580

Query: 548 GIAALNFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAEL 575
            +  +N  YF + A G+  K  +        +  +D E+
Sbjct: 581 VLGVVNLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEM 613

BLAST of CcUC04G065860 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 405.6 bits (1041), Expect = 9.4e-112
Identity = 225/574 (39.20%), Postives = 351/574 (61.15%), Query Frame = 0

Query: 13  PRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 72
           P KK   GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G T
Sbjct: 11  PEKKL--GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLT 70

Query: 73  NFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGETN-CPQP 132
           NFAPL GA ++DAY+GRF T+   S+ S +G+  +TL A LPQ+ PPPC     + C  P
Sbjct: 71  NFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDP 130

Query: 133 HPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIAL 192
           +  QL  L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ L
Sbjct: 131 NKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVL 190

Query: 193 VVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVA 252
           + + T+VVY+QT +SW +GF+IPT+    ++++F  G +FYV   P+GSVF+ +A+V+VA
Sbjct: 191 IFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVA 250

Query: 253 TCRKHGVPKPTQID----HLHNPPMNS---CKLAHTDRFLVFDKAATVIDSSMELDEEGK 312
             +K  + K + +D      + PP+      KL  TD+F   DKAA ++D   +L  EG 
Sbjct: 251 ARKKRDL-KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGV 310

Query: 313 SKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGPHFQI 372
             N+WRLCS+ +VE+ KC++ ++PVW  GI   ++M    +F + QA +M+R +GPHF+I
Sbjct: 311 PANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEI 370

Query: 373 PPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGI 432
           P A +++   I + IW+ IYE   V F+ +  +   R+++  R+ IGIV ++L M  AG 
Sbjct: 371 PAASITVISYITIGIWVPIYEHLLVPFLWRMRK--FRVTLLQRMGIGIVFAILSMFTAGF 430

Query: 433 LEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGA 492
           +E +RR  A E    ++++ V+ L     L GL E+F  I ++E   +  PE +R++A +
Sbjct: 431 VEGVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANS 490

Query: 493 IFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAALNFF 552
           +F LS + A+YLSS+L+  V K+   +     WL   DL++ +L+YF+Y++  +  +N  
Sbjct: 491 LFPLSFAAANYLSSLLVTTVHKVSGTK-DHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLV 550

Query: 553 YFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           YF + A  +  K          ++ L D E  +R
Sbjct: 551 YFWYCAHRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of CcUC04G065860 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.6e-103
Identity = 205/547 (37.48%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 14  RKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTN 73
           +KK    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT N
Sbjct: 39  QKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTIN 98

Query: 74  FAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCIT-GETNCPQPH 133
           F     AFL D Y GR+ TL +  IA F+G  V+ L A +PQ+ P  C T  ++ C  P 
Sbjct: 99  FGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPS 158

Query: 134 PWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALV 193
             Q++FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A +
Sbjct: 159 GGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQI 218

Query: 194 VALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVAT 253
           ++LT+VVYVQ+N+SWT+G  IP      + LIF +G + YV     GS  A +A+V+   
Sbjct: 219 LSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVA 278

Query: 254 CRKHGVPKPTQ----IDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELDEEGKSKNE 313
            +K G+    Q    + + + P   + KL +TD+F   DKAA ++    +L  +GK  + 
Sbjct: 279 IKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 338

Query: 314 WRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP-HFQIPPA 373
           W+LC++ QVE+ KCIV ++P+W      ++++ Q  ++ + QA+Q +R +G   F IP A
Sbjct: 339 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 398

Query: 374 WMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGILEK 433
              +  M  ++++I +Y++  V  M++ T  +  +++  RI  GI  +   +VVAG +E+
Sbjct: 399 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 458

Query: 434 LRRDAAV--------ENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLR 493
            RR  A+          K  +S +    LIP+ +L+G+ EAFAAI  ME      PE++R
Sbjct: 459 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 518

Query: 494 TVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIA 546
           + AG+IF++   ++SYL S L+  V +  +   G   WL   DLNK RL+ F++++ GI 
Sbjct: 519 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGG-NWL-AEDLNKGRLDLFYFMIAGIL 578

BLAST of CcUC04G065860 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-101
Identity = 206/554 (37.18%), Postives = 338/554 (61.01%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           M+N    SS D   ++  P GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V 
Sbjct: 1   MDNEKGTSSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQ 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           +  +   W   TNFAP+ GAF++D+Y G+F T++ GSIA  +GM VLT  + +P +RPPP
Sbjct: 61  AVTLINTWSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLE 180
           C T +    Q  P+  S LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    
Sbjct: 121 C-TADQITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSR 180

Query: 181 SFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAP 240
           SF +W+Y   +I  +V++T+V+YVQ NISW +GFAIPT    F++L+   G ++YV   P
Sbjct: 181 SFFSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKP 240

Query: 241 QGSVFADMAKVVVATCRKHGVPKPTQIDHLHNP----PMNSCKLAHTDRFLVFDKAATVI 300
           +GSVF+ + KV+VA  +K      + ID+ H P     + S KL  TD+F   +KA  V+
Sbjct: 241 EGSVFSGVFKVLVAAYKKRKARFTSGIDY-HQPLLETDLQSNKLVLTDQFRFLNKAVIVM 300

Query: 301 DSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQ 360
           ++    DE G    EWR C+V Q+E  K I+ IIP++ + I  F++M Q  +F + QA++
Sbjct: 301 NN----DEAG--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALK 360

Query: 361 MN-RFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGI 420
           M+ +F G  + IPPA +++  ++ + IW+  YE   V  ++  T+ N  +S+  ++ IG 
Sbjct: 361 MDLQFPGTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGN 420

Query: 421 VMSVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTT 480
           + S+  M+++GI+E+ RRD ++       ++ V+ L P+  L G  + F  + + E    
Sbjct: 421 IFSISTMLISGIVERKRRDLSLNG----VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNK 480

Query: 481 HLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFF 540
            +P ++R++  ++ +L LSLASYLSS ++ IV  +    GG+  WL  +D++K++L+ F+
Sbjct: 481 QVPINMRSIGNSLLYLGLSLASYLSSAMVSIVHSV-TARGGQ-SWL-TDDIDKSKLDCFY 539

Query: 541 YVVGGIAALNFFYF 546
           Y +  ++ LNF +F
Sbjct: 541 YFIAALSTLNFIFF 539

BLAST of CcUC04G065860 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.8e-265
Identity = 457/577 (79.20%), Postives = 514/577 (89.08%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           M+NG+L SSLD P  K   GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV 
Sbjct: 1   MDNGTLRSSLD-PSPKPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNVD 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLL GSIASF+GMGVLTLGA LP+MRPPP
Sbjct: 61  SAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180
           C +G+T+C Q   WQL+FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL
Sbjct: 121 CSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFL 180

Query: 181 NWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGS 240
           NWWYFLFSIALV+ALT+VVY+QTNISWTLGFAIPTA F+FSI+IFL G   Y+C+ PQGS
Sbjct: 181 NWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQGS 240

Query: 241 VFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELD 300
           VFADMAKV++AT RK  +P+P+  +HLH+PPM+S KL HT+RFLVFDKAA VIDSSMELD
Sbjct: 241 VFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMELD 300

Query: 301 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 360
           E+G+SKNEW+LC+VHQVEQ KC+VGI+P+W  GI CFISMQQMGSFGILQAIQMNRF+GP
Sbjct: 301 EQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLGP 360

Query: 361 HFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVV 420
           HF+IPPAWMSL PMIALS+WIYIYEKYVEFM++KT +NKR SMK RIEIGIVMSVLCM+V
Sbjct: 361 HFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMIV 420

Query: 421 AGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTV 480
           AGILEK+RRD+A+EN++FVS LHV +LIPEFALSGLTEAFAAI+VMELLTT LPESLRTV
Sbjct: 421 AGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRTV 480

Query: 481 AGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAAL 540
           AGAIFFLSLSLASYLSSVLMGIV+++ +    +  WL GNDLNKN L+YFFYVVG IAAL
Sbjct: 481 AGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAAL 540

Query: 541 NFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           NFFYFRF    FLP+ DVDPR KA +R+ ED E G R
Sbjct: 541 NFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of CcUC04G065860 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.5e-253
Identity = 446/585 (76.24%), Postives = 496/585 (84.79%), Query Frame = 0

Query: 1   MENGSL-HSSLDAP---RKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNL 60
           MEN ++ HSSLD P     KA  GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQM 120
           DNVASA V  IWGGTTNFAP+AGAFLADAYLGRFYTLL GSIASF GMGVLTLGAGLP++
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPPCITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180
           RPP C   +  CPQP PWQL+FLYLGLGL+VIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKA 240
           ESFLNWWYFLFSIAL+VALT+VVY+QTNISW  GFAIPTACFV SILIFL G   Y+ K 
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFADMAKVVVATCRKHGVPKPTQIDHLHNPP---MNSCKLAHTDRFLVFDKAATVI 300
           PQGSVFADMAKV++ATCRK  + KP   ++LH+PP    +S KL HT+RFLVFDKAATV+
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQ 360
           D   ELDE G S + WRLCSV QVEQ KC++GI+PVW+ GI CFI+MQQMGSFG+LQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSMKTRIEIGIVM 420
           M+RF+G HF+IPPAWM+LTPMIALSIWI++YEKYV  MK+KT  NKR+SM+ RIEIGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHL 480
           SV+CMVV+GI EKLRRD AV N SFV+RLHV  L PEFALSGLTEAFAAIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIG-RMEGGEIQWLGGNDLNKNRLEYFFY 540
           PESLRTVAGAIFFLSLSLASYLSSVL+GI+SKI   ++G +  W+GGNDLN NRLEYFFY
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVGGIAALNFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           VV  I  LNFFYFRFFAAGFLPK DVD   + H+RQLED E GRR
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETGRR 585

BLAST of CcUC04G065860 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 8.8e-246
Identity = 424/474 (89.45%), Postives = 448/474 (94.51%), Query Frame = 0

Query: 104 MGVLTLGAGLPQMRPPPCITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 163
           MGVLTLGAGLPQMRPPPCI+GE+NCPQP PWQLSFLYLGLGLIVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 164 QFDTNTEKGRAKLESFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSIL 223
           QFDTNTEKGRAKLESFLNWWYFLFS+ALV+ALTIVVYVQTN+SWTLGF IPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 224 IFLSGCQFYVCKAPQGSVFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRF 283
           IFL G ++Y+CKAPQGSVFAD+AKVVVATCRK  +PKPTQ D LHNPPMNS KLAHT+RF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 284 LVFDKAATVIDSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQM 343
           L+FDKAATV+DSS+ELDE+GKSKNEWRLCSVHQVE+FKC+VGIIPVWL GIPCF+SMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 344 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSM 403
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MKKKTR+NKR SM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 404 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAI 463
           KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSF+S LHVW+LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 464 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLN 523
           AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVL GIVSK+ R      +WLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVER------EWLGGNDLN 420

Query: 524 KNRLEYFFYVVGGIAALNFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           KNRL+YFFYVVG IAALNFFYFRFFA  FLPKPDVDPRQKAHQRQLEDAELGRR
Sbjct: 421 KNRLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of CcUC04G065860 vs. ExPASy TrEMBL
Match: A0A6J1GFB3 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111453646 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 4.0e-230
Identity = 396/456 (86.84%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 104 MGVLTLGAGLPQMRPPPCITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 163
           MGVLTLGAG P+MRPPPC  GE+NCPQPHPWQL FLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 164 QFDTNTEKGRAKLESFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSIL 223
           QFDTNTEKGRAKLESFLNWWY LFS+ALV+ALT+VVYVQT+ISWTLGFAIPTACFVFSIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 120

Query: 224 IFLSGCQFYVCKAPQGSVFADMAKVVVATCRKHGVPKPTQIDH-LHNPPMNSCKLAHTDR 283
           IFL G +FY+CK PQGSVFAD+AKV+VATCRK  VPK TQIDH LH+PPM+S KL+HTDR
Sbjct: 121 IFLLGRRFYICKTPQGSVFADIAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDR 180

Query: 284 FLVFDKAATVIDSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQ 343
           FLVFDKAATV+DSS ELDE+GKSKNEW+LCSVHQVEQFKC+VGI+P+W  GIPCFISMQQ
Sbjct: 181 FLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWAAGIPCFISMQQ 240

Query: 344 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLS 403
           MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKT  NKR S
Sbjct: 241 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFS 300

Query: 404 MKTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAA 463
           +KTRIEIGIVMSVLCMVVAGILE +RR +A ENKSFVS LHVWMLIPEFALSGLTEAFAA
Sbjct: 301 LKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAA 360

Query: 464 IAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDL 523
           IAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVSKI R+EGG+  WLGGNDL
Sbjct: 361 IAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDL 420

Query: 524 NKNRLEYFFYVVGGIAALNFFYFRFFAAGFLPKPDV 559
           NKNRL+YFFYVVG IAA NFFYFRFFA  FLPKP+V
Sbjct: 421 NKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 456

BLAST of CcUC04G065860 vs. ExPASy TrEMBL
Match: A0A6J1IS88 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111478233 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.8e-230
Identity = 394/455 (86.59%), Postives = 422/455 (92.75%), Query Frame = 0

Query: 104 MGVLTLGAGLPQMRPPPCITGETNCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 163
           MGVLTLGAG P+MRPPPC  GE+NCPQPHPWQL FLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 164 QFDTNTEKGRAKLESFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSIL 223
           QFDTNTEKGRAKLESFLNWWY LFS+ALV+ALT+VVY+QT+ISWTLGFAIPTACFVFSIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 224 IFLSGCQFYVCKAPQGSVFADMAKVVVATCRKHGVPKPTQIDHLHNPPMNSCKLAHTDRF 283
           IFL G +FY+CK PQGSVFADMAKV+VATCRK  VPK TQIDHL++PP++S KLAHTDRF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 284 LVFDKAATVIDSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQM 343
           LVFDKAATV DSS ELDE+GKSKNEW+LCSVHQVEQ KC+VGI+P+W  GIPCFISMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 344 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTRNNKRLSM 403
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKT  NKR SM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 404 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAI 463
           KTRIEIGIVMSVLCMVVAGILE +RR +AVENK FVS LHVW LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 464 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLN 523
           AVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVSKI R+EGG+ +WLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 524 KNRLEYFFYVVGGIAALNFFYFRFFAAGFLPKPDV 559
           KNRL+YFFYVVG IAA NFFYFRFFA  FLPKP+V
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of CcUC04G065860 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 572.0 bits (1473), Expect = 5.4e-163
Identity = 294/544 (54.04%), Postives = 382/544 (70.22%), Query Frame = 0

Query: 16  KATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 75
           K   GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 76  PLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGETNCPQPHPWQ 135
            LAGAF++DAYLGRF+TLLLGSIASF+GMG+  L A LP +RP  CI       QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 136 LSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALVVAL 195
           L  L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F++ALV+AL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 196 TIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVATCRK 255
           T VVY+QTNISW +GF IPTAC   SI  F+ G   Y+C   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 256 HGVPKPTQIDHLHNP-----PMNSCKLAHTDRFLVFDKAATVIDSSMELDEEGKSKNEWR 315
             V   + I     P     P    +  H  RF  FDK A+++ +  EL+E+G +K +WR
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRF--FDK-ASIVTNPNELNEDGNAKYKWR 314

Query: 316 LCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGPH-FQIPPAWM 375
           LCSV QV+  KC+  I+PVW+TGI CFI   Q   +GILQA+QM++  GPH FQ+P  WM
Sbjct: 315 LCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWM 374

Query: 376 SLTPMIALSIWIYIYE-KYVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGILEKLR 435
           +L  MI L+IWI +YE   +  +K+ T   KRL++K RIE  IVM ++CM+VAG  EK R
Sbjct: 375 NLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKR 434

Query: 436 RDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLS 495
           R +A++N SFVS + + ML+P+FAL+GLTEAF+A+A+ME LT  +PE +R VAGAIFFLS
Sbjct: 435 RASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLS 494

Query: 496 LSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAALNFFYFRFF 553
            S+ASY+ ++L+ ++  + R EG    WLG  DLNKNRLE +F+++ GI   N  YFR F
Sbjct: 495 SSIASYICTLLINVIDAVTRKEGK--SWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLF 551

BLAST of CcUC04G065860 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 412.9 bits (1060), Expect = 4.2e-115
Identity = 218/579 (37.65%), Postives = 356/579 (61.49%), Query Frame = 0

Query: 8   SSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 67
           S LDA + +  PGGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  I
Sbjct: 41  SILDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINI 100

Query: 68  WGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGE-T 127
           W G TN  PL GA+++D Y+GRF T+   S A+ +G+  +TL A  PQ+ P  C + +  
Sbjct: 101 WSGFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL 160

Query: 128 NCPQPHPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFL 187
           +C  P+  Q+  L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  
Sbjct: 161 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 220

Query: 188 FSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMA 247
           F++ L++  T+VVY+Q  +SW +GF+IPT     ++++F +G + YV   P+GS+F+ +A
Sbjct: 221 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 280

Query: 248 KVVVATCRKHGVPKPTQID---HLHNPPMNS---CKLAHTDRFLVFDKAATVIDSSMELD 307
           +V+VA  +K  +  P + D     ++P + S    KL  +++F   DKAA VI+   +L 
Sbjct: 281 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLT 340

Query: 308 EEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP 367
            EG   ++WRLCSV +VE+ KC++ I+P+W  GI    +M   G+F + QA++M+R +GP
Sbjct: 341 PEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGP 400

Query: 368 HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMV 427
            F+IP   +S+  ++ + I++  Y++ +V FM++ T +   +++  RI  GIV ++  M+
Sbjct: 401 KFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMI 460

Query: 428 VAGILEKLRRDAAVE--NKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESL 487
           VAGI+E++RR  ++   + + ++ + V+ L P+  L GL EAF  I  +E   +  PE +
Sbjct: 461 VAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHM 520

Query: 488 RTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQ--WLGGNDLNKNRLEYFFYVVG 547
           R++A ++F LS + +SYLSS L+ +V K     GG  +  WL  N LN  +L+YF+Y++ 
Sbjct: 521 RSIANSLFSLSFAGSSYLSSFLVTVVHK---FSGGHDRPDWLNKN-LNAGKLDYFYYLIA 580

Query: 548 GIAALNFFYFRFFAAGFLPKPDVDPRQKAHQRQLEDAEL 575
            +  +N  YF + A G+  K  +        +  +D E+
Sbjct: 581 VLGVVNLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEM 613

BLAST of CcUC04G065860 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 405.6 bits (1041), Expect = 6.7e-113
Identity = 225/574 (39.20%), Postives = 351/574 (61.15%), Query Frame = 0

Query: 13  PRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 72
           P KK   GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G T
Sbjct: 11  PEKKL--GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLT 70

Query: 73  NFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCITGETN-CPQP 132
           NFAPL GA ++DAY+GRF T+   S+ S +G+  +TL A LPQ+ PPPC     + C  P
Sbjct: 71  NFAPLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDP 130

Query: 133 HPWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIAL 192
           +  QL  L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ L
Sbjct: 131 NKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVL 190

Query: 193 VVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVA 252
           + + T+VVY+QT +SW +GF+IPT+    ++++F  G +FYV   P+GSVF+ +A+V+VA
Sbjct: 191 IFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVA 250

Query: 253 TCRKHGVPKPTQID----HLHNPPMNS---CKLAHTDRFLVFDKAATVIDSSMELDEEGK 312
             +K  + K + +D      + PP+      KL  TD+F   DKAA ++D   +L  EG 
Sbjct: 251 ARKKRDL-KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGV 310

Query: 313 SKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGPHFQI 372
             N+WRLCS+ +VE+ KC++ ++PVW  GI   ++M    +F + QA +M+R +GPHF+I
Sbjct: 311 PANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEI 370

Query: 373 PPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGI 432
           P A +++   I + IW+ IYE   V F+ +  +   R+++  R+ IGIV ++L M  AG 
Sbjct: 371 PAASITVISYITIGIWVPIYEHLLVPFLWRMRK--FRVTLLQRMGIGIVFAILSMFTAGF 430

Query: 433 LEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGA 492
           +E +RR  A E    ++++ V+ L     L GL E+F  I ++E   +  PE +R++A +
Sbjct: 431 VEGVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANS 490

Query: 493 IFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIAALNFF 552
           +F LS + A+YLSS+L+  V K+   +     WL   DL++ +L+YF+Y++  +  +N  
Sbjct: 491 LFPLSFAAANYLSSLLVTTVHKVSGTK-DHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLV 550

Query: 553 YFRFFAAGFLPKPDVDPRQKAHQRQLEDAELGRR 578
           YF + A  +  K          ++ L D E  +R
Sbjct: 551 YFWYCAHRYQYKAGSQIEDFNEEKSLLDIEPNQR 570

BLAST of CcUC04G065860 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.5e-104
Identity = 205/547 (37.48%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 14  RKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTN 73
           +KK    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT N
Sbjct: 39  QKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTIN 98

Query: 74  FAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPPCIT-GETNCPQPH 133
           F     AFL D Y GR+ TL +  IA F+G  V+ L A +PQ+ P  C T  ++ C  P 
Sbjct: 99  FGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPS 158

Query: 134 PWQLSFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALV 193
             Q++FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ A +
Sbjct: 159 GGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQI 218

Query: 194 VALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAPQGSVFADMAKVVVAT 253
           ++LT+VVYVQ+N+SWT+G  IP      + LIF +G + YV     GS  A +A+V+   
Sbjct: 219 LSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVA 278

Query: 254 CRKHGVPKPTQ----IDHLHNPPMNSCKLAHTDRFLVFDKAATVIDSSMELDEEGKSKNE 313
            +K G+    Q    + + + P   + KL +TD+F   DKAA ++    +L  +GK  + 
Sbjct: 279 IKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADP 338

Query: 314 WRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQMNRFIGP-HFQIPPA 373
           W+LC++ QVE+ KCIV ++P+W      ++++ Q  ++ + QA+Q +R +G   F IP A
Sbjct: 339 WKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAA 398

Query: 374 WMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGIVMSVLCMVVAGILEK 433
              +  M  ++++I +Y++  V  M++ T  +  +++  RI  GI  +   +VVAG +E+
Sbjct: 399 TYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEE 458

Query: 434 LRRDAAV--------ENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLR 493
            RR  A+          K  +S +    LIP+ +L+G+ EAFAAI  ME      PE++R
Sbjct: 459 RRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMR 518

Query: 494 TVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFFYVVGGIA 546
           + AG+IF++   ++SYL S L+  V +  +   G   WL   DLNK RL+ F++++ GI 
Sbjct: 519 SFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGG-NWL-AEDLNKGRLDLFYFMIAGIL 578

BLAST of CcUC04G065860 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-102
Identity = 206/554 (37.18%), Postives = 338/554 (61.01%), Query Frame = 0

Query: 1   MENGSLHSSLDAPRKKATPGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVA 60
           M+N    SS D   ++  P GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V 
Sbjct: 1   MDNEKGTSSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQ 60

Query: 61  SAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLLGSIASFMGMGVLTLGAGLPQMRPPP 120
           +  +   W   TNFAP+ GAF++D+Y G+F T++ GSIA  +GM VLT  + +P +RPPP
Sbjct: 61  AVTLINTWSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPP 120

Query: 121 CITGETNCPQPHPWQLSFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLE 180
           C T +    Q  P+  S LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    
Sbjct: 121 C-TADQITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSR 180

Query: 181 SFLNWWYFLFSIALVVALTIVVYVQTNISWTLGFAIPTACFVFSILIFLSGCQFYVCKAP 240
           SF +W+Y   +I  +V++T+V+YVQ NISW +GFAIPT    F++L+   G ++YV   P
Sbjct: 181 SFFSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKP 240

Query: 241 QGSVFADMAKVVVATCRKHGVPKPTQIDHLHNP----PMNSCKLAHTDRFLVFDKAATVI 300
           +GSVF+ + KV+VA  +K      + ID+ H P     + S KL  TD+F   +KA  V+
Sbjct: 241 EGSVFSGVFKVLVAAYKKRKARFTSGIDY-HQPLLETDLQSNKLVLTDQFRFLNKAVIVM 300

Query: 301 DSSMELDEEGKSKNEWRLCSVHQVEQFKCIVGIIPVWLTGIPCFISMQQMGSFGILQAIQ 360
           ++    DE G    EWR C+V Q+E  K I+ IIP++ + I  F++M Q  +F + QA++
Sbjct: 301 NN----DEAG--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALK 360

Query: 361 MN-RFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTRNNKRLSMKTRIEIGI 420
           M+ +F G  + IPPA +++  ++ + IW+  YE   V  ++  T+ N  +S+  ++ IG 
Sbjct: 361 MDLQFPGTSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGN 420

Query: 421 VMSVLCMVVAGILEKLRRDAAVENKSFVSRLHVWMLIPEFALSGLTEAFAAIAVMELLTT 480
           + S+  M+++GI+E+ RRD ++       ++ V+ L P+  L G  + F  + + E    
Sbjct: 421 IFSISTMLISGIVERKRRDLSLNG----VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNK 480

Query: 481 HLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEGGEIQWLGGNDLNKNRLEYFF 540
            +P ++R++  ++ +L LSLASYLSS ++ IV  +    GG+  WL  +D++K++L+ F+
Sbjct: 481 QVPINMRSIGNSLLYLGLSLASYLSSAMVSIVHSV-TARGGQ-SWL-TDDIDKSKLDCFY 539

Query: 541 YVVGGIAALNFFYF 546
           Y +  ++ LNF +F
Sbjct: 541 YFIAALSTLNFIFF 539