CcUC03G044920 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC03G044920
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationCicolChr03: 2865506 .. 2867501 (-)
RNA-Seq ExpressionCcUC03G044920
SyntenyCcUC03G044920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATATTGACCATAATCATGAACTTGAGGAAGTTTCACTTTGTTTCATTTTCCATCGTTAAATTTTACCAATACGCATCATGCAGACGTATAGTACGCAAACTATTAGAACTGAATTCGTTTTAAAGACTAATAATCAAAACCACTTTCTAATTAGTTTTCCATTCTTCTACACCAAAGTTTGGTTGCCAATTATTACCAATCAACATTCAGTTTTCATATTCCTTTGTTCTGAAAATTGGAATCCAAACCCAAAGCCATGGGATCAGAAGCCTGGGCTTTCACCAAACAAGTCATAGAAGGACGATGGTTTTCAGTATTCGCCGGAATGATGATGATGATCGGCAATGGTTCAACATACATTTATGGAACATACTCCAAAGTCGTCAAGACCGAATTTAATTACAGCCAAACCCAAATTAACACATTAGGATTCGCCAAAGATCTCGGTTCCAACGCCGGAATTTTCGCCGGCCTTCTCGCCGAGGTGGTTCCCACGTGTGTTCTCTTCATGATCGGTGCTGCCCAAAACTTCACTGGCTACTTCTTCATATGGCTGGCCATCACACGTCGTATCCCTCAACCAAAATTCTGGCAAATGTTCCTTTTCGTTTGTTTTGGAACTAATTCTTCTAATTTTTCCAATACAGGAATTATGGTCCATAGCGTCAGAAACTTCCCTGACCGTCGTGGTATCATTTTAGGGTTGCTTAAAGGGTATGTTGGTATTGGAGGTGCTATTTTAACCCAAGTTTATTTAGGAATATATGGCCATGAAGACCCATCAAATCTTGTTCTTCTTTTTGCTTGGTTTCCCACTACAGTAATTCTCCTTCTTTTCACCTCCATTCGACCAATTCGTATTCGTAAACATCCCGAAGAACTCAAAGTTTTTTACCATTTACTTTACGTCTCTGTTGTTCTAGCAATTTTCATCTTGTTTTTGACTATTACACAAAAAGAAGTTGTTTTCTCTCAAGCTGCGTACGTGAGTGGCGCGTCCGTCGTCGTTACCTTACTCTTTTTACCCCTTTTGATCGCTTGTAGAGAAGAATTCTTGTTGTACAAACTCAAGAAACAAAACTTTAACCTTGATCCTTCTGTTGCTCTATCCATTGTTGAACCAAAGATTTCTTCTCAAAACACCACTAACAAACCATTTCCCACATTGGAGGAAGTAGCAGAGATTTCGCCCACCACGTGTTTCGAAAACATATGCAACAAACCAGAACGAGGCGAAGATTTCACCATCTTGCAAGCCATCTTTAGCAAAGATATGGCTCTTATTTCTTTAGCTACTTTTTCTGGATGCGGGTCTTCTCTTGCAGCCATTGATAATTTAGGCCAAATTGGTGAATCACTTGGCTATCCACCACGAGCTATAAGCATATTGGTTTCATGGGTTTCCATATTCAATTTCTTTGGCAGAGTCTTCTCAGGTTTCATCTCCGAAAACTTGATTATCAAATACAGATTACCTCGTCCTTTAACATTTGCTTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGCATCGCGTATCCGTTTCCGGGTTCGGTCTACGTCGCTTCGATTGTTATCGGGTTCGGATTTGGAGCTCAAAATCCATTACTATTCGCGGTAATCTCAGAGATGTTTGGACTAAAACATTACGCCATATTGTTCAATTGTGGGCAATTGGTAGTCCCAATAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAATTGTATGATACAGAAGCTTTACGTGAAGGTAGAAAGATGACGGGCAAGGGGATAACTTGCACGGGAGCTCATTGTTTCAGTGGATCTTTCACAATTTTGGCTGCAGTGACATTGTTTGGAGCTTTGGTTATGCTTGTGTTGGCTTATCGGGCACGAGAATACTATAAATGGGATGTATATAAGAATTGCCGAGAGGATATATGGATTCCCCAAGAAGAAATGGAGTTTTATCGCTTAGACAACAGGAAGAACGTTGATGACTAA

mRNA sequence

ATGGGTATATTGACCATAATCATGAACTTGAGGAAGTTTCACTTTGTTTCATTTTCCATCGTTAAATTTTACCAATACGCATCATGCAGACAATTGGAATCCAAACCCAAAGCCATGGGATCAGAAGCCTGGGCTTTCACCAAACAAGTCATAGAAGGACGATGGTTTTCAGTATTCGCCGGAATGATGATGATGATCGGCAATGGTTCAACATACATTTATGGAACATACTCCAAAGTCGTCAAGACCGAATTTAATTACAGCCAAACCCAAATTAACACATTAGGATTCGCCAAAGATCTCGGTTCCAACGCCGGAATTTTCGCCGGCCTTCTCGCCGAGGTGGTTCCCACGTGTGTTCTCTTCATGATCGGTGCTGCCCAAAACTTCACTGGCTACTTCTTCATATGGCTGGCCATCACACGTCGTATCCCTCAACCAAAATTCTGGCAAATGTTCCTTTTCGTTTGTTTTGGAACTAATTCTTCTAATTTTTCCAATACAGGAATTATGGTCCATAGCGTCAGAAACTTCCCTGACCGTCGTGGTATCATTTTAGGGTTGCTTAAAGGGTATGTTGGTATTGGAGGTGCTATTTTAACCCAAGTTTATTTAGGAATATATGGCCATGAAGACCCATCAAATCTTGTTCTTCTTTTTGCTTGGTTTCCCACTACAGTAATTCTCCTTCTTTTCACCTCCATTCGACCAATTCGTATTCGTAAACATCCCGAAGAACTCAAAGTTTTTTACCATTTACTTTACGTCTCTGTTGTTCTAGCAATTTTCATCTTGTTTTTGACTATTACACAAAAAGAAGTTGTTTTCTCTCAAGCTGCGTACGTGAGTGGCGCGTCCGTCGTCGTTACCTTACTCTTTTTACCCCTTTTGATCGCTTGTAGAGAAGAATTCTTGTTGTACAAACTCAAGAAACAAAACTTTAACCTTGATCCTTCTGTTGCTCTATCCATTGTTGAACCAAAGATTTCTTCTCAAAACACCACTAACAAACCATTTCCCACATTGGAGGAAGTAGCAGAGATTTCGCCCACCACGTGTTTCGAAAACATATGCAACAAACCAGAACGAGGCGAAGATTTCACCATCTTGCAAGCCATCTTTAGCAAAGATATGGCTCTTATTTCTTTAGCTACTTTTTCTGGATGCGGGTCTTCTCTTGCAGCCATTGATAATTTAGGCCAAATTGGTGAATCACTTGGCTATCCACCACGAGCTATAAGCATATTGGTTTCATGGGTTTCCATATTCAATTTCTTTGGCAGAGTCTTCTCAGGTTTCATCTCCGAAAACTTGATTATCAAATACAGATTACCTCGTCCTTTAACATTTGCTTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGCATCGCGTATCCGTTTCCGGGTTCGGTCTACGTCGCTTCGATTGTTATCGGGTTCGGATTTGGAGCTCAAAATCCATTACTATTCGCGGTAATCTCAGAGATGTTTGGACTAAAACATTACGCCATATTGTTCAATTGTGGGCAATTGGTAGTCCCAATAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAATTGTATGATACAGAAGCTTTACGTGAAGGTAGAAAGATGACGGGCAAGGGGATAACTTGCACGGGAGCTCATTGTTTCAGTGGATCTTTCACAATTTTGGCTGCAGTGACATTGTTTGGAGCTTTGGTTATGCTTGTGTTGGCTTATCGGGCACGAGAATACTATAAATGGGATGTATATAAGAATTGCCGAGAGGATATATGGATTCCCCAAGAAGAAATGGAGTTTTATCGCTTAGACAACAGGAAGAACGTTGATGACTAA

Coding sequence (CDS)

ATGGGTATATTGACCATAATCATGAACTTGAGGAAGTTTCACTTTGTTTCATTTTCCATCGTTAAATTTTACCAATACGCATCATGCAGACAATTGGAATCCAAACCCAAAGCCATGGGATCAGAAGCCTGGGCTTTCACCAAACAAGTCATAGAAGGACGATGGTTTTCAGTATTCGCCGGAATGATGATGATGATCGGCAATGGTTCAACATACATTTATGGAACATACTCCAAAGTCGTCAAGACCGAATTTAATTACAGCCAAACCCAAATTAACACATTAGGATTCGCCAAAGATCTCGGTTCCAACGCCGGAATTTTCGCCGGCCTTCTCGCCGAGGTGGTTCCCACGTGTGTTCTCTTCATGATCGGTGCTGCCCAAAACTTCACTGGCTACTTCTTCATATGGCTGGCCATCACACGTCGTATCCCTCAACCAAAATTCTGGCAAATGTTCCTTTTCGTTTGTTTTGGAACTAATTCTTCTAATTTTTCCAATACAGGAATTATGGTCCATAGCGTCAGAAACTTCCCTGACCGTCGTGGTATCATTTTAGGGTTGCTTAAAGGGTATGTTGGTATTGGAGGTGCTATTTTAACCCAAGTTTATTTAGGAATATATGGCCATGAAGACCCATCAAATCTTGTTCTTCTTTTTGCTTGGTTTCCCACTACAGTAATTCTCCTTCTTTTCACCTCCATTCGACCAATTCGTATTCGTAAACATCCCGAAGAACTCAAAGTTTTTTACCATTTACTTTACGTCTCTGTTGTTCTAGCAATTTTCATCTTGTTTTTGACTATTACACAAAAAGAAGTTGTTTTCTCTCAAGCTGCGTACGTGAGTGGCGCGTCCGTCGTCGTTACCTTACTCTTTTTACCCCTTTTGATCGCTTGTAGAGAAGAATTCTTGTTGTACAAACTCAAGAAACAAAACTTTAACCTTGATCCTTCTGTTGCTCTATCCATTGTTGAACCAAAGATTTCTTCTCAAAACACCACTAACAAACCATTTCCCACATTGGAGGAAGTAGCAGAGATTTCGCCCACCACGTGTTTCGAAAACATATGCAACAAACCAGAACGAGGCGAAGATTTCACCATCTTGCAAGCCATCTTTAGCAAAGATATGGCTCTTATTTCTTTAGCTACTTTTTCTGGATGCGGGTCTTCTCTTGCAGCCATTGATAATTTAGGCCAAATTGGTGAATCACTTGGCTATCCACCACGAGCTATAAGCATATTGGTTTCATGGGTTTCCATATTCAATTTCTTTGGCAGAGTCTTCTCAGGTTTCATCTCCGAAAACTTGATTATCAAATACAGATTACCTCGTCCTTTAACATTTGCTTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGCATCGCGTATCCGTTTCCGGGTTCGGTCTACGTCGCTTCGATTGTTATCGGGTTCGGATTTGGAGCTCAAAATCCATTACTATTCGCGGTAATCTCAGAGATGTTTGGACTAAAACATTACGCCATATTGTTCAATTGTGGGCAATTGGTAGTCCCAATAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAATTGTATGATACAGAAGCTTTACGTGAAGGTAGAAAGATGACGGGCAAGGGGATAACTTGCACGGGAGCTCATTGTTTCAGTGGATCTTTCACAATTTTGGCTGCAGTGACATTGTTTGGAGCTTTGGTTATGCTTGTGTTGGCTTATCGGGCACGAGAATACTATAAATGGGATGTATATAAGAATTGCCGAGAGGATATATGGATTCCCCAAGAAGAAATGGAGTTTTATCGCTTAGACAACAGGAAGAACGTTGATGACTAA

Protein sequence

MGILTIIMNLRKFHFVSFSIVKFYQYASCRQLESKPKAMGSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAKDLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQAAYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPFPTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNLGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCIGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSYILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRAREYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD
Homology
BLAST of CcUC03G044920 vs. NCBI nr
Match: XP_004147308.1 (uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetical protein Csa_014134 [Cucumis sativus])

HSP 1 Score: 958.4 bits (2476), Expect = 3.1e-275
Identity = 477/577 (82.67%), Postives = 527/577 (91.33%), Query Frame = 0

Query: 39  MGSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFA 98
           MGSEAWAF KQV EGRWFSV+AGMMMMIGNG+TYI+GTYSKV+K+EF+YSQTQ+N LGFA
Sbjct: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60

Query: 99  KDLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCF 158
           KDLG+NAGI AGLL+E VPT VLFMIGA QNFTGYF IWL++TRRI QP FWQMFL VCF
Sbjct: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120

Query: 159 GTNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVL 218
           G+NSSN+SNT IMV S+RNFPDRRGIILGLLKGYVGIGGAILTQ+ LG YG EDPSN+VL
Sbjct: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180

Query: 219 LFAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQ 278
           LFAWFP+ +ILL+  SIRPI IRKHPEELKVFYHLLYVS+VLAIFILFLT+++K+VVFSQ
Sbjct: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240

Query: 279 AAYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKP 338
           +AY SGASVV+ LLFLPLLIACREEFLLYKLKKQN NL+PSV LSI++ K+ +   ++KP
Sbjct: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPN---SHKP 300

Query: 339 FPTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDN 398
           F TLEE+AEISP +C  NICNKP RGEDFTILQAIFS DM LI LATF+GCGSSLAAIDN
Sbjct: 301 FSTLEEIAEISP-SCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDN 360

Query: 399 LGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTC 458
           LGQIGESLGYPPRAI I VSWVSIFNFFGRV SGFISE ++IKY+LPRPL FAFAFLLTC
Sbjct: 361 LGQIGESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTC 420

Query: 459 IGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGS 518
           IGQLCIAYPFPGS+YVASIVIGFGFGAQNPLLFAVISEMFGLKHY+ILFNCGQL VP+GS
Sbjct: 421 IGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGS 480

Query: 519 YILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYR 578
           YILNVDIVGKLYD EALREG+KMTG+GI C+GAHCF GSFTILAA TLFGALVMLVLAYR
Sbjct: 481 YILNVDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYR 540

Query: 579 AREYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
            REYY+WDVYKN +ED+WIPQ EMEFYRLDN+KN+DD
Sbjct: 541 TREYYRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573

BLAST of CcUC03G044920 vs. NCBI nr
Match: XP_008464773.1 (PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo])

HSP 1 Score: 955.7 bits (2469), Expect = 2.0e-274
Identity = 478/577 (82.84%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 39  MGSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFA 98
           M SEAWAF KQV++GRWFSV+AGMMMMIGNG+TYI+GTYSKV+KTEF+YSQTQ+N LGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 99  KDLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCF 158
           KDLGSN GI AGLLAE VPT VLF IGA QNFT YFFIWL++TRRIPQP+FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 159 GTNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVL 218
           G+NSSN++NT IMV S+RNFPDRRGIILGLLKGYVGIGGAILTQ+YLGIYG EDPS+LVL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 219 LFAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQ 278
           LFAWFP+ +ILL+ TSIRPI IRKHPEELKVFYHLLYVS+++AIFI FL+ITQK+V FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 279 AAYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKP 338
           AAY SGA VVV LL LPLLI CREEFLLYKLKKQN N DPSVALSIV+ K+++     K 
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTN---CPKS 300

Query: 339 FPTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDN 398
           F TLEE+AEISP +C  NICNKP+RG DFT+LQAIFS DM LI LATFSGCGSSLAAIDN
Sbjct: 301 FSTLEEIAEISP-SCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDN 360

Query: 399 LGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTC 458
           LGQIGESLGYP RAISI VSWVSIFNFFGRVFSGFISE +IIKY+LPRPL FAFAFLLTC
Sbjct: 361 LGQIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTC 420

Query: 459 IGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGS 518
           +GQLCIAYPFPGSVYVAS +IGFGFGAQNPLLFAVISEMFGLKHY+ILFNCGQLVVP+GS
Sbjct: 421 LGQLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGS 480

Query: 519 YILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYR 578
           YILNVDIVGKLYD EALREGRKMTGKGITCTGAHCF GSFTILAA TLFGALVML+LAYR
Sbjct: 481 YILNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYR 540

Query: 579 AREYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
            R+YY+WD+YKN +ED+WIPQ EMEFYRLDNRKN+DD
Sbjct: 541 TRDYYRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

BLAST of CcUC03G044920 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 885.6 bits (2287), Expect = 2.5e-253
Identity = 444/576 (77.08%), Postives = 502/576 (87.15%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAW F KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF+IGA QNF G+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV+LL +P+LIACREE LLYKL KQ     PSV +S+V      QN    P 
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVH-----QNPPTLPA 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EISP+ C ENI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEISPSCC-ENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGYP RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTF+FAFL+TC+
Sbjct: 366 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGSVY+A+I+IGFGFGAQNP+LFAVISEMFGL+ Y+ LFNCGQL VP+GSY
Sbjct: 426 GQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EALREG+K+ GKG+TCTGAHCF GSFTILAA TLFGA+VMLVLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ +MEFYRLD++K+++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CcUC03G044920 vs. NCBI nr
Match: XP_022970240.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 884.0 bits (2283), Expect = 7.3e-253
Identity = 442/576 (76.74%), Postives = 501/576 (86.98%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAW F KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF++GA QNFTG+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q+YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV+LL +P+LIACREE LLYKL KQ     PSV +S+V      QN      
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVH-----QNPPTLSA 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EI P+ C ENI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEILPSCC-ENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGYP RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTF+FAFL+TC+
Sbjct: 366 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGSVY A+I+IGFGFGAQNP+LFAVISEMFGL+HY+ LFNCGQL VP+GSY
Sbjct: 426 GQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EAL EG+++ GKG+TCTGAHCF GSFTILAA TLFGA+VMLVLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ +MEFYRLDN+K+++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

BLAST of CcUC03G044920 vs. NCBI nr
Match: XP_022964780.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 882.1 bits (2278), Expect = 2.8e-252
Identity = 443/576 (76.91%), Postives = 500/576 (86.81%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAWAF KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF+IGA QNFTG+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q+YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP+ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV LL +P+LIACREE LLYKL K+     PSVALS++      QN    P 
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKE--TAAPSVALSMLH-----QNPPTLPT 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EISP+ C  NI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEISPSCC-PNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGY  RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTFAFAFL+TC+
Sbjct: 366 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGLK Y+ LFNCGQL  P GSY
Sbjct: 426 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EALRE +++ GKG+TCTGAHCF GSFT+LAA TLFGA+VM VLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ EMEFYRLDN+KN++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CcUC03G044920 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.0e-27
Identity = 135/544 (24.82%), Postives = 233/544 (42.83%), Query Frame = 0

Query: 54  RWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAKDLGSNAGIFAGLLA 113
           +W  + A + +    G+ + +  YS  +K+    SQ ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 114 EVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTNSSNFSNTGIMVH 173
              P  V+    AA  F GY   WL IT  I  P +  +FL       S  + NT   + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 174 SVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFAWFPTTVILLLFT 233
            +R+FP+ R + L L   + GI  A+ +  +  I  +   SNL LL        +++ F 
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLN--SLVPLVVSFA 222

Query: 234 SIRPIRIRKHPEELKVF------YHLLYVSVVLAIFILF-LTITQKEVVFSQAAYVSGAS 293
           ++ P+  +   +    +       H+  +  VLA+   F L ++      ++  ++    
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 294 VVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPFPTLEEVA 353
           ++V  L  PLL+  R+ FL     + N      V L+I E  + +Q T+           
Sbjct: 283 LLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDE--LKNQKTS----------- 342

Query: 354 EISPTTCFENI-----CNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNLGQ 413
            +S  T +E++      N    G++ +    I   +  L  +A F G    L   +NLGQ
Sbjct: 343 -VSSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQ 402

Query: 414 IGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCIG- 473
           I +SLG   +  + LV+  S F+FFGR+ S    + +  ++RL R   FA A L T I  
Sbjct: 403 IAQSLG---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAF 462

Query: 474 -QLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 533
             L ++     ++  A+ +IG   G       ++ S++FG     +  N     +PIGS 
Sbjct: 463 FLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSL 522

Query: 534 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 584
           +    I   +Y+  A  +   +    I C G  C+  +F     +++ G +  L L  R 
Sbjct: 523 LYGY-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRT 562

BLAST of CcUC03G044920 vs. ExPASy TrEMBL
Match: A0A1S3CMS0 (uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=4 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 9.6e-275
Identity = 478/577 (82.84%), Postives = 524/577 (90.81%), Query Frame = 0

Query: 39  MGSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFA 98
           M SEAWAF KQV++GRWFSV+AGMMMMIGNG+TYI+GTYSKV+KTEF+YSQTQ+N LGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 99  KDLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCF 158
           KDLGSN GI AGLLAE VPT VLF IGA QNFT YFFIWL++TRRIPQP+FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 159 GTNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVL 218
           G+NSSN++NT IMV S+RNFPDRRGIILGLLKGYVGIGGAILTQ+YLGIYG EDPS+LVL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 219 LFAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQ 278
           LFAWFP+ +ILL+ TSIRPI IRKHPEELKVFYHLLYVS+++AIFI FL+ITQK+V FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 279 AAYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKP 338
           AAY SGA VVV LL LPLLI CREEFLLYKLKKQN N DPSVALSIV+ K+++     K 
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTN---CPKS 300

Query: 339 FPTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDN 398
           F TLEE+AEISP +C  NICNKP+RG DFT+LQAIFS DM LI LATFSGCGSSLAAIDN
Sbjct: 301 FSTLEEIAEISP-SCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDN 360

Query: 399 LGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTC 458
           LGQIGESLGYP RAISI VSWVSIFNFFGRVFSGFISE +IIKY+LPRPL FAFAFLLTC
Sbjct: 361 LGQIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTC 420

Query: 459 IGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGS 518
           +GQLCIAYPFPGSVYVAS +IGFGFGAQNPLLFAVISEMFGLKHY+ILFNCGQLVVP+GS
Sbjct: 421 LGQLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGS 480

Query: 519 YILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYR 578
           YILNVDIVGKLYD EALREGRKMTGKGITCTGAHCF GSFTILAA TLFGALVML+LAYR
Sbjct: 481 YILNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYR 540

Query: 579 AREYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
            R+YY+WD+YKN +ED+WIPQ EMEFYRLDNRKN+DD
Sbjct: 541 TRDYYRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

BLAST of CcUC03G044920 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.2e-253
Identity = 444/576 (77.08%), Postives = 502/576 (87.15%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAW F KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF+IGA QNF G+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV+LL +P+LIACREE LLYKL KQ     PSV +S+V      QN    P 
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVH-----QNPPTLPA 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EISP+ C ENI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEISPSCC-ENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGYP RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTF+FAFL+TC+
Sbjct: 366 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGSVY+A+I+IGFGFGAQNP+LFAVISEMFGL+ Y+ LFNCGQL VP+GSY
Sbjct: 426 GQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EALREG+K+ GKG+TCTGAHCF GSFTILAA TLFGA+VMLVLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ +MEFYRLD++K+++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CcUC03G044920 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.5e-253
Identity = 442/576 (76.74%), Postives = 501/576 (86.98%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAW F KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF++GA QNFTG+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q+YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV+LL +P+LIACREE LLYKL KQ     PSV +S+V      QN      
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVH-----QNPPTLSA 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EI P+ C ENI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEILPSCC-ENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGYP RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTF+FAFL+TC+
Sbjct: 366 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGSVY A+I+IGFGFGAQNP+LFAVISEMFGL+HY+ LFNCGQL VP+GSY
Sbjct: 426 GQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EAL EG+++ GKG+TCTGAHCF GSFTILAA TLFGA+VMLVLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ +MEFYRLDN+K+++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

BLAST of CcUC03G044920 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.3e-252
Identity = 443/576 (76.91%), Postives = 500/576 (86.81%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAWAF KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF+IGA QNFTG+F IWL+IT RI +PKFWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q+YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP+ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV LL +P+LIACREE LLYKL K+     PSVALS++      QN    P 
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKE--TAAPSVALSMLH-----QNPPTLPT 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EISP+ C  NI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEISPSCC-PNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGY  RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTFAFAFL+TC+
Sbjct: 366 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGLK Y+ LFNCGQL  P GSY
Sbjct: 426 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EALRE +++ GKG+TCTGAHCF GSFT+LAA TLFGA+VM VLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ EMEFYRLDN+KN++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CcUC03G044920 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 3.0e-252
Identity = 443/576 (76.91%), Postives = 499/576 (86.63%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           GSEAWAF KQV+ GRWFSVFAG++MMIGNG+TY++ TYSKV+KT+F+YSQTQINTLGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLGSN GI AGLLAEV PT VLF+IGA QNFTG+F IWL+IT RI +PKFWQMF+ VCF 
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFA 125

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
           TNSSN++NT IMV SVRNFPDRRGIILGLLKGYVGIGGAI++Q+YL IYGH DPSNLVLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
           FAW P+ +IL+LF SIRPIRIRKHP+ELKVFY LLYVS++LAIFILFLTI QK+VVFSQA
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            YV GAS VV LL +P+LIACREE LLYKL KQ     PSVALS++      QN    P 
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKQ--TAAPSVALSMLH-----QNPPTLPT 305

Query: 340 PTLEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNL 399
               E+ EISP+ C  NI NKPERGEDFTILQA+FSKDMALI LATFSGCGSSLAAIDNL
Sbjct: 306 SADSEIPEISPSCC-PNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 365

Query: 400 GQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCI 459
           GQ+GESLGY  RAI ILVSWVSIFNFFGRVFSGFISE L+ KY+LPRPLTFAFAFL+TC+
Sbjct: 366 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 425

Query: 460 GQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSY 519
           GQL IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGLK Y+ LFNCGQL  P GSY
Sbjct: 426 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 485

Query: 520 ILNVDIVGKLYDTEALREGRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLAYRA 579
           ILNVD+VGKLYD EALRE +++ GKG+TCTGAHCF GSFT+LAA TLFGA+VM VLAYR 
Sbjct: 486 ILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRT 545

Query: 580 REYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVDD 616
           RE+YK DVYKN  E+IWIPQ EMEFYRLDN+KN++D
Sbjct: 546 REFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CcUC03G044920 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 560.1 bits (1442), Expect = 2.3e-159
Identity = 293/578 (50.69%), Postives = 390/578 (67.47%), Query Frame = 0

Query: 42  EAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAKDL 101
           E   F      GRWF VFA  ++M   G+TY++GTYSK +K+   Y QT +N LGF KDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 102 GSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTN 161
           G+N G+ +GL+AEV PT  +  IG+A NF GYF IWL +T ++ +PK WQM L++C G N
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 162 SSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFA 221
           S NF+NTG +V  V+NFP+ RG++LGLLKGYVG+ GAI TQ+Y  IYGH D  +L+LL A
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIA 184

Query: 222 WFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQAAY 281
           W P  V L+    IR  ++ +   EL VFY  LY+S+ LA+F++ + I +K+V FS+AAY
Sbjct: 185 WLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAY 244

Query: 282 VSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPFPT 341
            + A++   LLF+PL ++ ++E  ++ + K    ++    + + +PK       +K    
Sbjct: 245 AASATICCALLFVPLTVSVKQELEVWNMMK--LPIEEPSEVKVEKPKKELDLDQDKAAKV 304

Query: 342 LEEVAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDNLGQ 401
             E  E    +CF  + + P RGED+TILQA+ S DM ++ +ATF G GSSL A+DNLGQ
Sbjct: 305 NGEEKETK--SCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 364

Query: 402 IGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTCIGQ 461
           IGESLGYP   +S  VS VSI+N+FGRVFSGF+SE L+ KY+LPRPL      LL+C G 
Sbjct: 365 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 424

Query: 462 LCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGSYIL 521
           L IA+P PGSVY+ASI++GF FGAQ PLLFA+ISE+FGLK+Y+ LFNCGQL  P+GSYIL
Sbjct: 425 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 484

Query: 522 NVDIVGKLYDTEALRE--GRKMTGKGI---TCTGAHCFSGSFTILAAVTLFGALVMLVLA 581
           NV + G LYD EAL++   R +T K +   TC G+ C+   F ILAAVT FGALV L LA
Sbjct: 485 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLA 544

Query: 582 YRAREYYKWDVYKNCREDIWIPQEEMEFYRLDNRKNVD 615
            R RE+YK D+YK  RE    P+ E E    D+RK V+
Sbjct: 545 IRTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

BLAST of CcUC03G044920 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 518.5 bits (1334), Expect = 7.5e-147
Identity = 266/569 (46.75%), Postives = 372/569 (65.38%), Query Frame = 0

Query: 40  GSEAWAFTKQVIEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAK 99
           G    + T Q++ GRWF  F  +++M   G+TY++G YS  +K    Y QT +N L F K
Sbjct: 7   GGSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFK 66

Query: 100 DLGSNAGIFAGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFG 159
           DLG+N G+ AGLL EV P   + +IGA  NF GYF IWLA+T RI +P+ W M L++C G
Sbjct: 67  DLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVG 126

Query: 160 TNSSNFSNTGIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLL 219
            NS +F+NTG +V  V+NFP+ RG++LG+LKGYVG+ GAI+TQ+Y   YG ED   L+L+
Sbjct: 127 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILM 186

Query: 220 FAWFPTTVILLLFTSIRPIRIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQA 279
             W P  V      +IR +++++   ELKVFY+ LY+S+ LA F++ + I  K   F+Q+
Sbjct: 187 IGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQS 246

Query: 280 AYVSGASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTNKPF 339
            +   A+VV+ LL LP+++   EE  L+K K+   N    + +   +PK+ S    +   
Sbjct: 247 EFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDG 306

Query: 340 PTLEEVAE-ISPTTCFENICNKPERGEDFTILQAIFSKDMALISLATFSGCGSSLAAIDN 399
              +EV E +   +C+  + N PERG+D+TILQA+FS DM ++ LAT  G G +L AIDN
Sbjct: 307 EESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDN 366

Query: 400 LGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLTC 459
           LGQIG SLGYP R++S  VS VSI+N++GRV SG +SE  +IKY+ PRPL      LL+C
Sbjct: 367 LGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSC 426

Query: 460 IGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIGS 519
            G L IA+  PG +YVAS++IGF FGAQ PLLFA+ISE+FGLK+Y+ L+N G +  PIGS
Sbjct: 427 AGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGS 486

Query: 520 YILNVDIVGKLYDTEALREGR-----KMTGKGITCTGAHCFSGSFTILAAVTLFGALVML 579
           Y+LNV + G LYD EA ++ +     ++ G+ + C G  CF  SF I+AAVTLFG LV +
Sbjct: 487 YLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSM 546

Query: 580 VLAYRAREYYKWDVYKNCREDIWIPQEEM 603
           VL  R +++YK D+YK  RE     + EM
Sbjct: 547 VLVIRTKKFYKSDIYKKFREKALAAEMEM 574

BLAST of CcUC03G044920 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 3.9e-79
Identity = 187/561 (33.33%), Postives = 295/561 (52.58%), Query Frame = 0

Query: 51  IEGRWFSVFAGMMMMIGNGSTYIYGTYSKVVKTEFNYSQTQINTLGFAKDLGSNAGIFAG 110
           I  RW    A M +    G  Y++G+ S V+K+  NY+Q +++ LG AKDLG + G  AG
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 111 LLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTNSSNFSNTGI 170
            L+E++P     ++GA QN  GY ++WL +T R P    W M + +  G N   + NTG 
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 171 MVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFAWFPTTVILL 230
           +V  V+NFP  RG ++G+LKG+ G+GGAI++Q+Y  I+   +P++L+L+ A  P  V++ 
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVC 192

Query: 231 LFTSIRPIRIRK--HPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQAAYVSGASVV 290
           L   IRP+   K   P +   F  +  V ++LA +++ + + Q  VV S         V+
Sbjct: 193 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 252

Query: 291 VTLLFLPLLIACR--------------EEFLLYKLKKQNFNLD-PSVALSIVE---PK-- 350
             +L +P+L+                 EE L+ K + Q   L  P + LS VE   PK  
Sbjct: 253 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDV 312

Query: 351 -ISSQNTTNKPFPTLEE--VAEISPTTCFENICNKPERGEDFTILQAIFSKDMALISLAT 410
            +   +  +K    L+   +   +      N    P RGEDFT+ QA+   D  LI  + 
Sbjct: 313 DLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSL 372

Query: 411 FSGCGSSLAAIDNLGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLP 470
             G GS L  IDNLGQ+ +SLGY      +LVS +SI+NF GR+  G+ SE ++  Y  P
Sbjct: 373 LLGSGSGLTVIDNLGQMSQSLGYD--NTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYP 432

Query: 471 RPLTFAFAFLLTCIGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAI 530
           RP+  A A L+  +G +  AY +PG++Y+ +++IG G+GA   ++ A  SE+FGLK +  
Sbjct: 433 RPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 492

Query: 531 LFNCGQLVVPIGSYILNVDIVGKLYDTEALREGRKMT---GKGITCTGAHCFSGSFTILA 584
           L+N   L  P GS + +  I   +YD EA R+           + C G+ CF  +  I++
Sbjct: 493 LYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMS 552

BLAST of CcUC03G044920 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 2.2e-77
Identity = 183/547 (33.46%), Postives = 287/547 (52.47%), Query Frame = 0

Query: 50  VIEGRWFSVFAGMMMMIGNGSTYIY-GTYSKVVKTEFNYSQTQINTLGFAKDLGSNAGIF 109
           ++  RW      M +    G  Y++ G+ S  +KT   Y+Q QI  LG AK+LG   G  
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 110 AGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTNSSNFSNT 169
           +G L+EV P+ V+ ++GA QN  GY  +WL +T ++P    W +F+ +  GTN   + NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 170 GIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFAWFPTTVI 229
             +V  + NFP+ RG ++G+LKG+ G+ GAILTQVYL ++     S+++L+ A  P  V+
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVV 191

Query: 230 LLLFTSIRPI----RIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQAAYVSG 289
           L L   +RP+    R     ++L+ F  +    VVLA+++L L + Q     +Q    + 
Sbjct: 192 LALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTS 251

Query: 290 ASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTN-KPFPTLE 349
            +++V  + +P+L+     F                        IS  N T+ KP     
Sbjct: 252 GAILVIFMVVPVLVPFSSVF------------------------ISGNNVTSVKPEEGTS 311

Query: 350 EVAEISPTTCFENICNKPER------GEDFTILQAIFSKDMALISLATFSGCGSSLAAID 409
            V +    T  E     PE+      GEDFT+LQA+   D  LI ++   G GS +  ID
Sbjct: 312 NVDQHEARTLIERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIID 371

Query: 410 NLGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLT 469
           NLGQI  SLGY      I VS +SI NF GRV  G+ SE +I K  LPR L  +    + 
Sbjct: 372 NLGQICYSLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIM 431

Query: 470 CIGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIG 529
            +G +  A  +PG +YV +IVIG G+GA   +  A +S++FGLK +  L+N     +PIG
Sbjct: 432 SLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIG 491

Query: 530 SYILNVDIVGKLYDTEALRE-GRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLA 584
           S++ +  I   +YD  A ++ G     + + CTG+ C+S + ++++ + L   ++ L + 
Sbjct: 492 SFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVV 530

BLAST of CcUC03G044920 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 2.2e-77
Identity = 183/547 (33.46%), Postives = 287/547 (52.47%), Query Frame = 0

Query: 50  VIEGRWFSVFAGMMMMIGNGSTYIY-GTYSKVVKTEFNYSQTQINTLGFAKDLGSNAGIF 109
           ++  RW      M +    G  Y++ G+ S  +KT   Y+Q QI  LG AK+LG   G  
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 110 AGLLAEVVPTCVLFMIGAAQNFTGYFFIWLAITRRIPQPKFWQMFLFVCFGTNSSNFSNT 169
           +G L+EV P+ V+ ++GA QN  GY  +WL +T ++P    W +F+ +  GTN   + NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 170 GIMVHSVRNFPDRRGIILGLLKGYVGIGGAILTQVYLGIYGHEDPSNLVLLFAWFPTTVI 229
             +V  + NFP+ RG ++G+LKG+ G+ GAILTQVYL ++     S+++L+ A  P  V+
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVV 191

Query: 230 LLLFTSIRPI----RIRKHPEELKVFYHLLYVSVVLAIFILFLTITQKEVVFSQAAYVSG 289
           L L   +RP+    R     ++L+ F  +    VVLA+++L L + Q     +Q    + 
Sbjct: 192 LALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTS 251

Query: 290 ASVVVTLLFLPLLIACREEFLLYKLKKQNFNLDPSVALSIVEPKISSQNTTN-KPFPTLE 349
            +++V  + +P+L+     F                        IS  N T+ KP     
Sbjct: 252 GAILVIFMVVPVLVPFSSVF------------------------ISGNNVTSVKPEEGTS 311

Query: 350 EVAEISPTTCFENICNKPER------GEDFTILQAIFSKDMALISLATFSGCGSSLAAID 409
            V +    T  E     PE+      GEDFT+LQA+   D  LI ++   G GS +  ID
Sbjct: 312 NVDQHEARTLIERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIID 371

Query: 410 NLGQIGESLGYPPRAISILVSWVSIFNFFGRVFSGFISENLIIKYRLPRPLTFAFAFLLT 469
           NLGQI  SLGY      I VS +SI NF GRV  G+ SE +I K  LPR L  +    + 
Sbjct: 372 NLGQICYSLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIM 431

Query: 470 CIGQLCIAYPFPGSVYVASIVIGFGFGAQNPLLFAVISEMFGLKHYAILFNCGQLVVPIG 529
            +G +  A  +PG +YV +IVIG G+GA   +  A +S++FGLK +  L+N     +PIG
Sbjct: 432 SLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIG 491

Query: 530 SYILNVDIVGKLYDTEALRE-GRKMTGKGITCTGAHCFSGSFTILAAVTLFGALVMLVLA 584
           S++ +  I   +YD  A ++ G     + + CTG+ C+S + ++++ + L   ++ L + 
Sbjct: 492 SFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVV 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147308.13.1e-27582.67uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetica... [more]
XP_008464773.12.0e-27482.84PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo][more]
XP_022970365.12.5e-25377.08protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote... [more]
XP_022970240.17.3e-25376.74protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_022964780.12.8e-25276.91protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4I9E12.0e-2724.82Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CMS09.6e-27582.84uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=... [more]
A0A6J1I5D71.2e-25377.08protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A6J1I2B33.5e-25376.74protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A6J1HLT11.3e-25276.91protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HP703.0e-25276.91protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G28120.12.3e-15950.69Major facilitator superfamily protein [more]
AT2G39210.17.5e-14746.75Major facilitator superfamily protein [more]
AT5G14120.13.9e-7933.33Major facilitator superfamily protein [more]
AT5G50630.12.2e-7733.46Major facilitator superfamily protein [more]
AT5G50520.12.2e-7733.46Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 54..299
e-value: 1.2E-75
score: 254.3
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 378..610
e-value: 1.0E-10
score: 43.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 56..580
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 399..581
e-value: 4.6E-12
score: 45.5
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 39..594
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 39..594

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC03G044920.1CcUC03G044920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity