CcUC03G042520 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC03G042520
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionPseudoU_synth_1 domain-containing protein
LocationCicolChr03: 236681 .. 243031 (-)
RNA-Seq ExpressionCcUC03G042520
SyntenyCcUC03G042520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGTCTCTTTTCTGCGACTTCCGCTTTCTCCATGGACAACCAAAACTTCATCTTCATGCTTTCCTTTTCCCTACCCTGCTTTCGTTTCGCCGACCAATCGTCTTTGGCCTTCACTTCCAAGCTGTTCCTCATCTCCTACTGCGTCCACCGATGCTTTTTCCACTCAGTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTCCGTAAGAAGAAGGTTGTTTTGCGGATCGGCTATGTTGGCACTGATTATAGAGGTCTCATTTCATATTACTCGCTGTTTCTCTTGACGAAGCTGTTGTGAATGTTTCACCAGTTCATTAACTCTTGCGTTTTAAAAATTCAGGGCTGCAAATGCAACGCGAGCAACAATCACATCCAAGTAAGTTTTTTGTGTGCATTTCTTCTTACATTACTAATATAATGTCAGGAAAATGCCTATCGGTGGATCGGCAATGGCATCCTCTGAATATTCTGTTGCCGTGTTTGATTTGTATACTTTCACAGTGAATGTGATGGTGGTTTGCTCACTCAATTACTTACTTAGCCAAATGTAGGGTACGACAACTAATTTAGTTCACAGTGACTAACTGAAATAATTGGGTATTCACTAAGCAATTAACAACTTTATAGATGATTAATTACCTTTTAACATTTTAACTATTTAACCACGTAATTACTTAACATTCCCCTGGCAAGCTCGGTGTAGGGGGGAGTCAACAATGGACCTCAAACAGCAGTTGACTACTGAAAGTGGCTTTGTGATCATATCGGACGTCTTATCACGTGAAGGGACATTTTGAATTGCTAATGAACCAAGTGGAATTTTCTTAAGCACAACGTCTTTCATTCAAGAATGTTGAACTGAATTTGCACTTAAAAGCACCGAAGTTAAGCTCTGACACACTAGCGAATGGGATCACGTGAAGGAGCAGTATTGATCTCATGCAGTGAGGATTGCATCCATGCAAGCTTAGTTGTAATATTGGCAACTTAGTTTCTGTATTTAAGAGTGCCTAACTCAGATTGTGTCGAAATCAATCGTGGTCATTAGAGTTATAGCTCCATCTGCATCAGCTAATCTATAATAAAGACCAGTTTAACAGCACCTTTGAAGAACCACAAAATATGTTTCGCAGCCTTTTATTGAATCTCTAACTATAAGGGGTGTTTGGATGCCCAGATTTTGGCATGGGATTGGGTTTTATAAACCCATTATTGCATTTGGCTTTACATTATGTCATATGAGTTTGGATTTTTGTAGCCTTCATGCTATCATTCAATACCATCGAAAAATCATGGGATTACTTAACATCGCAGGCAGTTTTTCAATAAAGGATGACTATTGGAGCTCACCCTGCTGCATAACTAGTGCAAGGAATTTCATTTTTCTCAAATAGTTTTACATTAATAAACTGCCTTTATTTTTTTTCTTGAAGTTGATCTTTGTACTGCTTGACCTTCCTTTACACGTCATAGTGAATACCTTTTCCAAAGTACTCCAGATGCAGAAATATTCACTACTTGAGTCCTAATGCTTTCAATTGTTGAAGCAAATAATCATGACCATTGTTTTTCTTAGCTCCAAAGCCGGGAATCTGGATTTTGACTTGACTTATCACCATTTTTAAGTCTTAGGGAAGGGGGGCATTTGGCTTTAGAGGAGTTGTAGAATTTTTCGTAGGATCGAGTGGTTTGGGAGAATCTTTGGGACGTGTGGGGACAATTTTTTGTAATTATCAACTTGATATAATTCTTTTGGATTGGATCCCCTTTTGTAGTTGGGCATGAGGGCCGTTTTATTTTTCTGGCTTGCTTTTTGTATTGCTCTTGTATAGTCTTTCATCTATCTCAATGAAAGTTTGTTTGTTTGTTTTATATATATGCATATATAAGGACTCATCAATAAAACATTCTTAGTTGAAGCTATTTATTCCAACTAGCACTTGAAACATTCACAGAAGGAACCTTTTATCAGCCTTCCTATGATGTACTGACAGATTAGATCCCATGTATTGAAGACGATGCAGTCTAATAGGCAAATTCCCAAGGGGAAGTGTTAAACAAGCTTGCTGTTACATGCTTTGGGAGAGCTAAAGATGTGTGGCAGAATAGTTAGTGCTAAAGGAGTAGATAAAAAGGACACGTATAAGCAACAACAATTTCATTACAATCTAATTGCATAATGATCGACAGAAAGAATCGAACTTAGTATGCATGTGAGCAGATCAGCAACTGATTAAATACACTCAAACTGATTATCCACTTAACCATTTAACTAACCTTGACGTTGATATATTATTTTATGTACATTAATCACAAAGGATCTGACGAAGACTTCCATTTATTTTATTTAGTCATTTATTGATTTTCTCTTATGTAAATTCTCAGTTCTCTCACCTGAGACAATATAACCAACTGAAGAAGGCTATAGATACATCCATCCTGTTTACTTTTCCCGTACATTGACAAATATGTTATGCACATTGCCCTTCTTTACACTCAAGAATAGAAAAGCTGACTGCACTGGATAACCACTGCTTATTTTTTCACCCTTCTCTAATTATCCTAGATAAGCATTTCTATGCCTTTATGTTTTCCTTATAATTCACTTTTGTTTAGATTGTGTTGTTCTGTCTTAATATTCCATTAATCATTATTTTTCTAGTTTCTAATTTTGGTTTGTTGTAATTTTGAGCGTTACCCTCTTTTCATTATATCAATGAAAAGTGTTTGTTGTTCCTTTAAAAAAAATAATATAGTACTAATAAGCATGGCATATGAATAATACTATTTCTCTCGCTCACTCTCTCTCTCTCTCCTTTCCTTTTTCTTCTTTTTGCTGGGAATTACAACACAGAAGTTAACCTCATGCAGTTTTGATGTATAGCAATAGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACCGATAAAGGAGTAAATATCTTGAATGTCTTTGTTGTAACTCATTTTATGACTTCTATAATCTTATTGGTTGTTTTGATTTGTTTTATCATCTCCTTCATTTTCAAGGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGATCCTTGTGGTATTTCGCTTGCTAATTACATCAATTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGTGAGTGTTCGAGTTTTTTTCACCGGTGCTTTTATGTTAGTTAATGAGGTCTTATCATTAGCCGGATACCCTTTAATGTATTTACTACAAAGTGGTAACATTACATCAATTTTGTCACTTGTTAAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACCGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGTAGGTAATTATCTCCTTCATTTAATTACATATAGCTCAGATTGCCCCTCCCTTCGCTGCAAGAAACATGATTTGTTAGATTTGATATAGTATAGTACTGTTATTTGGGAAGAATGGTGTTGAAGAACAAAGGTGGGTTCTTTTTGGGTACAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGAATTCAAACCAAACGATCTCCAGGACATGCCCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTAAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAGCTCGGAGATGGGTCTGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCACGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAAGTTAGTCTTTCTTTACCTTTGTTTTTATGGTTCATAATTTGTTCTCATCCATTCTCGTTCCAATTTGCAAGTTTATCTGTTTTTACTCCATTTATTCTTTTATTTCAGTTGGCAGCTCTACTACTTAGATTTGGGTTTCCCATTCTAACACCTCTGGTAGCTTAGATCTTGCTTCTGGCCATGTGGGAGCCCAAACTTGGTTTTATAACTGTTCTTGTGTCCACTCGACAATTTTTTCGTAGCATTATTTCACTTTTTAAAGTCTTTTCCCCCCTTCAGAAATCAATGCTTAGTGACCTAGTGGGAGTTCCTGTGCTAGTAGTCCTTCGAAAAGTTAAGAAATAGCCATGGTCATATGAAATTAAGTTACATTCTAAAATATTATGAATGATTAAATAATTTGATGCCCCTATGCTTTGGTGGACGGAAGATATGAGTTTATCGCTTCAAGCTTTTTGAGCTTGACTTGTTCAATATCTACCATCATAACCTTCATTTACTTCCTGGGGTTCACAATTTTTAGGATCAGCATGCGCCATTAGACATATCTACCATAACTTTTCTGCAGTTGCCTATTGCTATTTGAAATTAAATTCTAATAGACTGAGAACCATTAAATAATTTCATGGTCCCTGTGATGCATTGGTGGACAGAAGATACGAGTTGATGGGTTCAAGCTTTATGAGCTTGACTTGTTAAATATCTACCATCATAACCTTTATTTATTCCCTGAGGTTCATAATTCATGGGATCAGCTTACACCATTAGACATGTCTAGTTTAACTTTTCTGCACTTAACTATCGCTATTTGAAATCAATTCTTATTATCTGGTTTAGAAGGTGGAAATAGAAACTCTATCGAGACAATTTGATTAATGATTTGTTTGAAAACCTTTCTCATCTCTGTTAGAGACTAAACTATTCCACGCAGGTCAAAGCCTCAGGAAGAAACCCAAAATCTCATGGGGAAACGGTACAAAAAGTGGAAATCCTGATCGGATAGAAACTAGAATCTATTAGAGTAACTTTAAAATTTGTTTGAAGAGCTTTCGCTTCTCTGTTGGAGATTAAACCATTCCATTGAGTTAAAGCCTGAAGAAGAATCCCAGAATCTTATGGACATGGTACCAAGGGGCGCTAACTTGAGATATCTAACCCTCTTTGAGGTAGAGGTTCAAATTCACACTACTACATTTGTTGTACTCAAAAGTGTTACAAAGGAAAAAGCATTATTGCAGCAGATTAGGCAGAGAGAAACTAGAATCTATTAGTAACTTTTGGCAGATTTCTCATTTCCATTTTTTTTGTACGTTTGAGCTCGTAATATGATTTTTTTCCTATTAATACATAACATCAATAACATATCCTGTGAACAATGAAGTTGGCTTCACTAGTAATTAATATTTGGTAGTCAATGTTCTATTTTCGAATCGAAAGGGCCATTCTGATGCGACTTTATTACTTCCTCGGCAGATTCGGAAAATGATAGGAACAGCCATTGCAGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGACCAGGAGTTCCAAAGAGGCCAGACTTGCTCACACTGAATGAATCAGATGACATTGTGAAGTCGGTGAATGAATTCTACAGATCATCGATACTGCCCGAGGTAACTAACTTCCTGGACACAGAAAGACCCCCTTGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGATGAAGTTAGGAATGCTTGGAAAGCATGGAACTGCAGGGTACATTGAATTGTGTTGATGAGTCTTTTCTTGGAAGTAGTAAAAAACTTGAGTATTGAAGAGGATGGGAGGTAGTTGAGTGGAGTTAGGGGAGTTATTAATGGTACAAAATTATGGGAATTGGGCATTGAATGGATGTGATTTTTGCGGCCACCCACCAGCCGTGGGGCGGCGTTTAATAACACAGCATCAACTACAGACAACCCACTCGGCACTCGGCACTCGGCACTCGGCACAACCATAAGGGAGTGAGCTCGCATTGAATGTAAAGGGCTTAAAAGGCGAAATATGTAGGTGAGCCATTGACAGGGATGTACTGATGTTGATTTAACTTGATCTGTGATGACAATTTGTATCAAAATTTGACTAATGATACCCATTCCGA

mRNA sequence

ATGACAGTCTCTTTTCTGCGACTTCCGCTTTCTCCATGGACAACCAAAACTTCATCTTCATGCTTTCCTTTTCCCTACCCTGCTTTCGTTTCGCCGACCAATCGTCTTTGGCCTTCACTTCCAAGCTGTTCCTCATCTCCTACTGCGTCCACCGATGCTTTTTCCACTCAGTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTCCGTAAGAAGAAGGTTGTTTTGCGGATCGGCTATGTTGGCACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAACAATCACATCCAACAATAGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACCGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGATCCTTGTGGTATTTCGCTTGCTAATTACATCAATTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACCGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGAATTCAAACCAAACGATCTCCAGGACATGCCCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTAAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAGCTCGGAGATGGGTCTGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCACGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAATTGGCAGCTCTACTACTTAGATTTGGGTTTCCCATTCTAACACCTCTGATTCGGAAAATGATAGGAACAGCCATTGCAGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGACCAGGAGTTCCAAAGAGGCCAGACTTGCTCACACTGAATGAATCAGATGACATTGTGAAGTCGGTGAATGAATTCTACAGATCATCGATACTGCCCGAGGTAACTAACTTCCTGGACACAGAAAGACCCCCTTGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGATGAAGTTAGGAATGCTTGGAAAGCATGGAACTGCAGGGTACATTGAATTGTGTTGATGAGTCTTTTCTTGGAAGTAGTAAAAAACTTGAGTATTGAAGAGGATGGGAGGTAGTTGAGTGGAGTTAGGGGAGTTATTAATGGTACAAAATTATGGGAATTGGGCATTGAATGGATGTGATTTTTGCGGCCACCCACCAGCCGTGGGGCGGCGTTTAATAACACAGCATCAACTACAGACAACCCACTCGGCACTCGGCACTCGGCACTCGGCACAACCATAAGGGAGTGAGCTCGCATTGAATGTAAAGGGCTTAAAAGGCGAAATATGTAGGTGAGCCATTGACAGGGATGTACTGATGTTGATTTAACTTGATCTGTGATGACAATTTGTATCAAAATTTGACTAATGATACCCATTCCGA

Coding sequence (CDS)

ATGACAGTCTCTTTTCTGCGACTTCCGCTTTCTCCATGGACAACCAAAACTTCATCTTCATGCTTTCCTTTTCCCTACCCTGCTTTCGTTTCGCCGACCAATCGTCTTTGGCCTTCACTTCCAAGCTGTTCCTCATCTCCTACTGCGTCCACCGATGCTTTTTCCACTCAGTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTCCGTAAGAAGAAGGTTGTTTTGCGGATCGGCTATGTTGGCACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAACAATCACATCCAACAATAGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACCGATAAAGGAGTTCATTCCTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGATCCTTGTGGTATTTCGCTTGCTAATTACATCAATTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACCGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAGAATTCAAACCAAACGATCTCCAGGACATGCCCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTGTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTAAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAGCTCGGAGATGGGTCTGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCACGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCGTTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAATTGGCAGCTCTACTACTTAGATTTGGGTTTCCCATTCTAACACCTCTGATTCGGAAAATGATAGGAACAGCCATTGCAGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGACCAGGAGTTCCAAAGAGGCCAGACTTGCTCACACTGAATGAATCAGATGACATTGTGAAGTCGGTGAATGAATTCTACAGATCATCGATACTGCCCGAGGTAACTAACTTCCTGGACACAGAAAGACCCCCTTGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCCAATGAACAATTGGATGAAGTTAGGAATGCTTGGAAAGCATGGAACTGCAGGGTACATTGA

Protein sequence

MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAPDQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPEVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH
Homology
BLAST of CcUC03G042520 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 946.0 bits (2444), Expect = 1.3e-271
Identity = 473/525 (90.10%), Postives = 486/525 (92.57%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LRLPLSPW TKTSSSCF FPYP FVS T+RL P+L  CS+S TASTDAFSTQCAP
Sbjct: 1   MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           +QLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTI LKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQ+SFDPRKECDVRKYSY+LPAKLIGI +KLKTTEVD HISDFKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRTRI+ KRSPG+ H    EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSKYRTRIRAKRSPGYGH----EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLSVCYENPTEVCSKLGDGSELPK VRARWLHEPDEKDRIGASHFRKIL CSCGKLETSF
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKMIGTAIAVKRKLLPRD+IALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMIGTAIAVKRKLLPRDVIALSLS 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
           VTNFLDTERPPWKEWVEKLDKYTSIP+EQLDEVRNAWKAWN RVH
Sbjct: 481 VTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRVH 505

BLAST of CcUC03G042520 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 904.0 bits (2335), Expect = 5.8e-259
Identity = 453/525 (86.29%), Postives = 475/525 (90.48%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M+VS LRLPLSPW TK+SSSCF FPYP FV  T RL P+L  C SS  ASTDAFSTQCAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRS YRTRI+ K+SPG+A KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+IL CSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDF+EISIWGESFMLHQ                IRKMIGTA+AVKRKLLPRD+IALSLT
Sbjct: 361 GYDFIEISIWGESFMLHQ----------------IRKMIGTAVAVKRKLLPRDVIALSLT 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGNSFS RKRPGVP RPDL+TLNESDDI+KSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
           VT FLDTE PPWKEWVEKLDKYTSIPNE+LDEVR+AWKAW  RVH
Sbjct: 481 VTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

BLAST of CcUC03G042520 vs. NCBI nr
Match: XP_022964935.1 (putative tRNA pseudouridine synthase [Cucurbita moschata])

HSP 1 Score: 892.5 bits (2305), Expect = 1.8e-255
Identity = 444/520 (85.38%), Postives = 468/520 (90.00%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW  KTS  CF FPYP FVS T+RL P L  CSSS TASTDA STQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRTRI+T +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKM+GTAIAVKRKLLPRDIIALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLS 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 504

BLAST of CcUC03G042520 vs. NCBI nr
Match: XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 890.2 bits (2299), Expect = 8.7e-255
Identity = 444/520 (85.38%), Postives = 465/520 (89.42%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW TKTS  CF FPYP FV  T+RL P L  CSSS TASTDA STQCAP
Sbjct: 18  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 78  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRTRI+  +SPG+ HKSG+ VS SD SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGA HFRKILYCSCGKLETS 
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKM+GTAIAVKRKLLPRDIIALSLT
Sbjct: 378 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLT 437

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIV+PIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 438 KFSRIVMPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 497

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 498 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 521

BLAST of CcUC03G042520 vs. NCBI nr
Match: XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])

HSP 1 Score: 887.9 bits (2293), Expect = 4.3e-254
Identity = 444/521 (85.22%), Postives = 468/521 (89.83%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW TKTS  CF FPYP FVS T+RL P L  CSSS TASTDA STQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
           NYTIRSKYRTRI+  +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV  G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLS C EN  EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 FGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSL 420
            GYDFVEISIWGESFMLHQ                IRKM+GTAIAVKRKLLPRDIIALSL
Sbjct: 361 LGYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSL 420

Query: 421 TKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILP 480
           +KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLN+SDDIVKSVNEFYRS+ILP
Sbjct: 421 SKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILP 480

Query: 481 EVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           EVTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 EVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 505

BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 4.1e-138
Identity = 266/527 (50.47%), Postives = 354/527 (67.17%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTN--RLWPSLPSCSSSPTASTDAFSTQC 60
           M VSFLR+P+              P   F  PT   R+ P+L   SSS  A+    ST  
Sbjct: 1   MAVSFLRIPI--------------PSRVFWYPTRLIRVTPALLRFSSS--AAFQPSSTSL 60

Query: 61  APDQ-----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGG 120
           +P         KWE++RKKKVV+RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGG
Sbjct: 61  SPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGG 120

Query: 121 IRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLP 180
           IR+SNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW  DP G  LA  I+ +LP
Sbjct: 121 IRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLP 180

Query: 181 DNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSF 240
           +NI+VF ++PS R FDPR+EC +RKYSYLLP  ++GI++   + E+D HI+DF +IL  F
Sbjct: 181 ENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEF 240

Query: 241 QGEHPFHNYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVK 300
           +GE+PFHNYT RS+YR + + K       K  N   P +  + +  ES+  ++  +E  +
Sbjct: 241 EGEYPFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGE 300

Query: 301 EPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSC 360
           E +++     V  E+   V +   D S++    RA+WL+EPDE D+I  +HFRK+  C  
Sbjct: 301 EEEEK----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRS 360

Query: 361 GKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRD 420
           GKLE S G+ FVEISIWGESFMLHQ                IRKMIGTA+AVKR+LLPRD
Sbjct: 361 GKLENSLGFGFVEISIWGESFMLHQ----------------IRKMIGTAVAVKRELLPRD 420

Query: 421 IIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFY 480
           II LSL KF+RIVLP+AP+EVL+LRGNSF  R+   +P+RP +    ES+++ K + EFY
Sbjct: 421 IIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFY 480

Query: 481 RSSILPEVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           R+ ++P+V+ FLD+E+ PWKEWV+ LD+   + +E+L++VR  W+ W
Sbjct: 481 RAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEW 480

BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-19
Identity = 110/423 (26.00%), Postives = 188/423 (44.44%), Query Frame = 0

Query: 71  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV   IGY GT Y G+Q+   Q+   TIE ++  A  KAG I ++N  +  K ++ R+
Sbjct: 84  KRKVACMIGYCGTGYHGMQLNPPQK---TIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 143

Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LKM I +          ++   IN +LP+ ++V+G+  + ++F+ 
Sbjct: 144 ARTDKGVHAAGNVISLKMIIEDE---------NIVEKINSHLPEQLRVWGVSRTNKAFEC 203

Query: 191 RKECDVRKYSYLLPA--------KLIGIESKLK--TTEVDCHISDFKDILNSFQGEHPFH 250
           RK C  R Y YL+P           +  E  LK  T+  +     ++ +    + +   +
Sbjct: 204 RKLCSSRVYEYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEEGKKYWESVAADLESQGVSY 263

Query: 251 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 310
           +  ++      I+           G E    +V+ SE +     D   +E +K  ++R  
Sbjct: 264 DEWMKRACIDEIK-----------GEET--KEVAESEVKTDSKTDAATLEKIKAVERRHR 323

Query: 311 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 370
                +    E  +K+ +  ++ +G    + +    K     S  R +   +C       
Sbjct: 324 EE---FRISGERLAKIREILKIYEGTH-NFHNFTLGKAFKDPSAMRTMKSLTCSDPFLID 383

Query: 371 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIG-TAIAVKRKLLPRDIIALSL 430
           G ++V I I G+SFMLHQ                IRKMI   A++V+    P+ +I  + 
Sbjct: 384 GTEWVSIKIHGQSFMLHQ----------------IRKMISMVALSVRCNADPQKLIPQTF 443

Query: 431 TKFSRIVLPIAPAEVLVLRGNSFS--NRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSI 481
            K +RI +P APA  L+L    +   N+K  G   R  +   N +D I    ++F    I
Sbjct: 444 EK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIYDKI 460

BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match: Q755C8 (tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 91/321 (28.35%), Postives = 144/321 (44.86%), Query Frame = 0

Query: 71  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+KV + +GY GT Y G+Q     +   TIE EL  A  KAG I  +N  +L K  + R+
Sbjct: 66  KRKVAVMVGYCGTGYHGMQYNPPNR---TIEAELFEAFVKAGAISRANSTDLKKNGFMRA 125

Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
           +RTDKGVH+    I+LK+ I + A         + + IN +LP  I+V+GI    ++FD 
Sbjct: 126 ARTDKGVHAGGNVISLKLIIEDPA---------IKDKINEHLPPGIRVWGISRVNKAFDC 185

Query: 191 RKECDVRKYSYLLPA-KLIGIE--SKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSK 250
           RK C  R Y YLLP    IG +  + L  T   C  +       S + +    +      
Sbjct: 186 RKLCGSRWYEYLLPTYSFIGPKPNTYLARTIEQCGEA------ASEKPDRDQESLDFWEA 245

Query: 251 YRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYE 310
           +R  +  K +        N V+P   S  +F+E+ G      + VK+  Q   +    Y 
Sbjct: 246 FRKAVDEKFTQEEQDAIVNYVAP---SKEDFDENSG----LYQKVKQYKQMENAHRRSYR 305

Query: 311 NPTEVCSKLGDGSELPKGVR--ARWLHEPDEKDRIGASHFRKILYCS---CGKLETSFGY 370
             +   ++  +  +   G      +    D KD       + I        G+++T    
Sbjct: 306 VSSAKLARFREAMKQYLGPHNFHNYTLGKDFKDPSTVRFMKDITVSDPFVIGEMKT---- 357

Query: 371 DFVEISIWGESFMLHQLAALL 384
           ++V I I G+SFMLHQ+  ++
Sbjct: 366 EWVSIKIHGQSFMLHQIRKMI 357

BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 55/180 (30.56%), Postives = 94/180 (52.22%), Query Frame = 0

Query: 71  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+K+VL + Y G  Y G+Q         TIE +L +A+ +AG I E++  ++ K+S+ R 
Sbjct: 79  KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 138

Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
           +RTDKGV +    ++LK+      W+ D     + + IN +LP +I++ G+      F+ 
Sbjct: 139 ARTDKGVSAAGQVVSLKV------WLID----DILDKINSHLPSHIRILGLKRVTGGFNS 198

Query: 191 RKECDVRKYSYLLPA-------KLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYT 244
           + +CD R Y Y+LP        + +  ES   + E    +     +L  ++G H FHN+T
Sbjct: 199 KNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAET---LQQVNRLLGCYKGTHNFHNFT 245

BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 4.2e-18
Identity = 55/180 (30.56%), Postives = 94/180 (52.22%), Query Frame = 0

Query: 71  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+K+VL + Y G  Y G+Q         TIE +L +A+ +AG I E++  ++ K+S+ R 
Sbjct: 79  KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 138

Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
           +RTDKGV +    ++LK+      W+ D     + + IN +LP +I++ G+      F+ 
Sbjct: 139 ARTDKGVSAAGQVVSLKV------WLID----DILDKINSHLPSHIRILGLKRVTGGFNS 198

Query: 191 RKECDVRKYSYLLPA-------KLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYT 244
           + +CD R Y Y+LP        + +  ES   + E    +     +L  ++G H FHN+T
Sbjct: 199 KNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAET---LQQVNRLLACYKGTHNFHNFT 245

BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 453/525 (86.29%), Postives = 475/525 (90.48%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M+VS LRLPLSPW TK+SSSCF FPYP FV  T RL P+L  C SS  ASTDAFSTQCAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRS YRTRI+ K+SPG+A KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+IL CSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDF+EISIWGESFMLHQ                IRKMIGTA+AVKRKLLPRD+IALSLT
Sbjct: 361 GYDFIEISIWGESFMLHQ----------------IRKMIGTAVAVKRKLLPRDVIALSLT 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGNSFS RKRPGVP RPDL+TLNESDDI+KSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
           VT FLDTE PPWKEWVEKLDKYTSIPNE+LDEVR+AWKAW  RVH
Sbjct: 481 VTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.5e-256
Identity = 444/520 (85.38%), Postives = 468/520 (90.00%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW  KTS  CF FPYP FVS T+RL P L  CSSS TASTDA STQCAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRTRI+T +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKM+GTAIAVKRKLLPRDIIALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLS 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 504

BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 444/521 (85.22%), Postives = 468/521 (89.83%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW TKTS  CF FPYP FVS T+RL P L  CSSS TASTDA STQCAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
           NYTIRSKYRTRI+  +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV  G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
           PSLS C EN  EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 FGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSL 420
            GYDFVEISIWGESFMLHQ                IRKM+GTAIAVKRKLLPRDIIALSL
Sbjct: 361 LGYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSL 420

Query: 421 TKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILP 480
           +KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLN+SDDIVKSVNEFYRS+ILP
Sbjct: 421 SKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILP 480

Query: 481 EVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           EVTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 EVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 505

BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/520 (82.50%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW TKTSS+CF  PY AFVS +  L P L  C SSP+ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK  TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYR  I+ ++SPG+ H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
            LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKM+GTA+AVKRKLLPRDIIALSLT
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAVAVKRKLLPRDIIALSLT 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGNSFSN KRPG PKRPDLLTLN+SDDI+KSV+EFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           +TNFLDTE+ PW EWVEKLDKYTSIPNEQLDEVRNAWKAW
Sbjct: 481 LTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAW 503

BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match: A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/520 (82.50%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
           M VS LR PLSPW TKTSS+CF  PY AFVS +  L P L  C SSP+ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK  TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYR  I+ ++SPG+ H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
            LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
           GYDFVEISIWGESFMLHQ                IRKM+GTA+AVKRKLLPRDIIALSLT
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAVAVKRKLLPRDIIALSLT 420

Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
           KFSRIVLPIAPAEVLVLRGNSFSN KRPG PKRPDLLTLN+SDDI+KSV+EFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPE 480

Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           +TNFLDTE+ PW EWVEKLDKYTSIPNEQLDEVRNAWKAW
Sbjct: 481 LTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAW 503

BLAST of CcUC03G042520 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.9e-139
Identity = 266/527 (50.47%), Postives = 354/527 (67.17%), Query Frame = 0

Query: 1   MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTN--RLWPSLPSCSSSPTASTDAFSTQC 60
           M VSFLR+P+              P   F  PT   R+ P+L   SSS  A+    ST  
Sbjct: 1   MAVSFLRIPI--------------PSRVFWYPTRLIRVTPALLRFSSS--AAFQPSSTSL 60

Query: 61  APDQ-----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGG 120
           +P         KWE++RKKKVV+RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGG
Sbjct: 61  SPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGG 120

Query: 121 IRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLP 180
           IR+SNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW  DP G  LA  I+ +LP
Sbjct: 121 IRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLP 180

Query: 181 DNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSF 240
           +NI+VF ++PS R FDPR+EC +RKYSYLLP  ++GI++   + E+D HI+DF +IL  F
Sbjct: 181 ENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEF 240

Query: 241 QGEHPFHNYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVK 300
           +GE+PFHNYT RS+YR + + K       K  N   P +  + +  ES+  ++  +E  +
Sbjct: 241 EGEYPFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGE 300

Query: 301 EPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSC 360
           E +++     V  E+   V +   D S++    RA+WL+EPDE D+I  +HFRK+  C  
Sbjct: 301 EEEEK----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRS 360

Query: 361 GKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRD 420
           GKLE S G+ FVEISIWGESFMLHQ                IRKMIGTA+AVKR+LLPRD
Sbjct: 361 GKLENSLGFGFVEISIWGESFMLHQ----------------IRKMIGTAVAVKRELLPRD 420

Query: 421 IIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFY 480
           II LSL KF+RIVLP+AP+EVL+LRGNSF  R+   +P+RP +    ES+++ K + EFY
Sbjct: 421 IIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFY 480

Query: 481 RSSILPEVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
           R+ ++P+V+ FLD+E+ PWKEWV+ LD+   + +E+L++VR  W+ W
Sbjct: 481 RAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEW 480

BLAST of CcUC03G042520 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-24
Identity = 111/412 (26.94%), Postives = 176/412 (42.72%), Query Frame = 0

Query: 64  RKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLN 123
           R+   +R++KV +   + G  Y+G+Q   +     TIE ELE A++ AG + +++     
Sbjct: 50  RRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPDADRNKPR 109

Query: 124 KISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVP 183
              WARS+RTDKGV ++   ++ +  +       DP G      +N  LPD I+VFG   
Sbjct: 110 NYEWARSARTDKGVSAVGQVVSGRFYV-------DPPG--FVERLNSKLPDQIRVFGYKR 169

Query: 184 SQRSFDPRKECDVRKYSYLLPAKLIG--IESKLKTTEVDCHISDFKDILNSFQG-EHPF- 243
              SF  +K CD R+Y YL+P   +   +  + +    D      K +  S +G + P  
Sbjct: 170 VAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTDLGYEYVKCVECSEKGYKIPLG 229

Query: 244 ---HNYTIRSKYRTRIQTKRSPGHAHKSGNEVS--------PSDVSNSEFEESDGEDDTF 303
               +          IQ+  S       G +V         PS  +N   E  DG D + 
Sbjct: 230 VMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCETLSSSVPSAENNLNSEVLDGADVSA 289

Query: 304 VEGVKEPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDR--------I 363
           +    E  + S +L+       +V    G+G ++ K   +++ +   EK+R        +
Sbjct: 290 LSSKAEGMEESNTLA-------KVEMNNGEGEDMTKS-ESKFCYGEKEKERFNRILSYYV 349

Query: 364 GASHF---------------RKILYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLR 423
           G+ +F               R IL  +   +    G DFV+  + G+SFMLHQ       
Sbjct: 350 GSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQ------- 409

Query: 424 FGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 438
                    IRKMIG A+A+ R   P  +I  S  K  RI +P+AP   L L
Sbjct: 410 ---------IRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLYL 425

BLAST of CcUC03G042520 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-20
Identity = 102/399 (25.56%), Postives = 168/399 (42.11%), Query Frame = 0

Query: 72  KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G    N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTR 251
           K CD R+Y YLLP   +   S      V   +   ++ +  F+          R +    
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201

Query: 252 IQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 311
               +  G    + +    SD+S++    S    D  +E +        S+ V      E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261

Query: 312 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 371
              KL   S   K V++++ +   EK+R        +G+ +F               R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321

Query: 372 LYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRK 431
           +  +   +    G DF++  + G+SFMLHQ                IRKM+G A+A+ R 
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQ----------------IRKMMGLAVAIMRN 381

Query: 432 LLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKR 448
                +I  + +K   I +P+AP   L L    F++  R
Sbjct: 382 CASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNR 382

BLAST of CcUC03G042520 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 5.6e-18
Identity = 94/373 (25.20%), Postives = 156/373 (41.82%), Query Frame = 0

Query: 72  KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 132 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 191
           RTDKGV ++        ++    ++ DP G    N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 192 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTR 251
           K CD R+Y YLLP   +   S      V   +   ++ +  F+          R +    
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201

Query: 252 IQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 311
               +  G    + +    SD+S++    S    D  +E +        S+ V      E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261

Query: 312 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 371
              KL   S   K V++++ +   EK+R        +G+ +F               R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321

Query: 372 LYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRK 422
           +  +   +    G DF++  + G+SFMLHQ                IRKM+G A+A+ R 
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQ----------------IRKMMGLAVAIMRN 356

BLAST of CcUC03G042520 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 3.4e-07
Identity = 57/225 (25.33%), Postives = 86/225 (38.22%), Query Frame = 0

Query: 71  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
           K+ V L+I Y G  + G     E Q  P+IE E    ++KA        G+     ++R 
Sbjct: 89  KRYVALKIMYFGKRFYG--FSAEAQMEPSIESE----VFKALERTRLLVGDKKDSCYSRC 148

Query: 131 SRTDKGVHSLATTITL----KMEIPE-------SAWMGDPCGISLANYINFYLPDNIKVF 190
            RTDKGV S    I L    +++ P        +   G+         +N  LPD+I+V 
Sbjct: 149 GRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVI 208

Query: 191 GIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPF 250
           G  P+   F  R  C  R+Y Y    + + + +            DF      F GEH F
Sbjct: 209 GWSPAPIDFHARFSCYAREYKYFFWRQNLNLSA-----------MDFAG--KKFIGEHDF 268

Query: 251 HNYTIRSKYRTRIQTKR------SPGHAHKSGNEVSPSDVSNSEF 279
            N+           T+R      SP      G+++    +  S F
Sbjct: 269 RNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAF 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894774.11.3e-27190.10putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida][more]
XP_008457308.15.8e-25986.29PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo][more]
XP_022964935.11.8e-25585.38putative tRNA pseudouridine synthase [Cucurbita moschata][more]
XP_023520301.18.7e-25585.38putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo][more]
XP_022970646.14.3e-25485.22putative tRNA pseudouridine synthase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O229284.1e-13850.47Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032... [more]
Q6CC391.7e-1926.00tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O... [more]
Q755C82.5e-1828.35tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... [more]
Q4KM922.5e-1830.56tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2[more]
Q9WU564.2e-1830.56tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3C5W12.8e-25986.29putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=... [more]
A0A6J1HM838.5e-25685.38putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1I1562.1e-25485.22putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569... [more]
A0A6J1DMV63.6e-24682.50putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DQ073.6e-24682.50putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT2G30320.12.9e-13950.47Pseudouridine synthase family protein [more]
AT1G20370.11.8e-2426.94Pseudouridine synthase family protein [more]
AT1G76120.11.6e-2025.56Pseudouridine synthase family protein [more]
AT1G76120.25.6e-1825.20Pseudouridine synthase family protein [more]
AT1G34150.13.4e-0725.33Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 195..509
e-value: 1.9E-27
score: 98.0
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 63..194
e-value: 1.4E-31
score: 111.1
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 124..250
e-value: 2.9E-15
score: 54.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 251..302
NoneNo IPR availablePANTHERPTHR11142:SF21TRNA PSEUDOURIDINE SYNTHASEcoord: 40..520
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 40..520
IPR041708Pseudouridine synthase PUS1/ PUS2-likeCDDcd02568PseudoU_synth_PUS1_PUS2coord: 76..442
e-value: 3.49103E-75
score: 235.971
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 342..444
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 71..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC03G042520.1CcUC03G042520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding