Homology
BLAST of CcUC03G042520 vs. NCBI nr
Match:
XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])
HSP 1 Score: 946.0 bits (2444), Expect = 1.3e-271
Identity = 473/525 (90.10%), Postives = 486/525 (92.57%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LRLPLSPW TKTSSSCF FPYP FVS T+RL P+L CS+S TASTDAFSTQCAP
Sbjct: 1 MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
+QLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTI LKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQ+SFDPRKECDVRKYSY+LPAKLIGI +KLKTTEVD HISDFKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYRTRI+ KRSPG+ H EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSKYRTRIRAKRSPGYGH----EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLSVCYENPTEVCSKLGDGSELPK VRARWLHEPDEKDRIGASHFRKIL CSCGKLETSF
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKMIGTAIAVKRKLLPRD+IALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMIGTAIAVKRKLLPRDVIALSLS 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
VTNFLDTERPPWKEWVEKLDKYTSIP+EQLDEVRNAWKAWN RVH
Sbjct: 481 VTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRVH 505
BLAST of CcUC03G042520 vs. NCBI nr
Match:
XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])
HSP 1 Score: 904.0 bits (2335), Expect = 5.8e-259
Identity = 453/525 (86.29%), Postives = 475/525 (90.48%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M+VS LRLPLSPW TK+SSSCF FPYP FV T RL P+L C SS ASTDAFSTQCAP
Sbjct: 1 MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRS YRTRI+ K+SPG+A KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+IL CSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDF+EISIWGESFMLHQ IRKMIGTA+AVKRKLLPRD+IALSLT
Sbjct: 361 GYDFIEISIWGESFMLHQ----------------IRKMIGTAVAVKRKLLPRDVIALSLT 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGNSFS RKRPGVP RPDL+TLNESDDI+KSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
VT FLDTE PPWKEWVEKLDKYTSIPNE+LDEVR+AWKAW RVH
Sbjct: 481 VTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508
BLAST of CcUC03G042520 vs. NCBI nr
Match:
XP_022964935.1 (putative tRNA pseudouridine synthase [Cucurbita moschata])
HSP 1 Score: 892.5 bits (2305), Expect = 1.8e-255
Identity = 444/520 (85.38%), Postives = 468/520 (90.00%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW KTS CF FPYP FVS T+RL P L CSSS TASTDA STQCAP
Sbjct: 1 MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
+LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYRTRI+T +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLS YEN EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKM+GTAIAVKRKLLPRDIIALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLS 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 504
BLAST of CcUC03G042520 vs. NCBI nr
Match:
XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 890.2 bits (2299), Expect = 8.7e-255
Identity = 444/520 (85.38%), Postives = 465/520 (89.42%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW TKTS CF FPYP FV T+RL P L CSSS TASTDA STQCAP
Sbjct: 18 MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 78 DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYRTRI+ +SPG+ HKSG+ VS SD SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLS YEN EVCSKLG+GSELP+GVRARWLHEPDEKDRIGA HFRKILYCSCGKLETS
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKM+GTAIAVKRKLLPRDIIALSLT
Sbjct: 378 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLT 437
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIV+PIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 438 KFSRIVMPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 497
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 498 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 521
BLAST of CcUC03G042520 vs. NCBI nr
Match:
XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])
HSP 1 Score: 887.9 bits (2293), Expect = 4.3e-254
Identity = 444/521 (85.22%), Postives = 468/521 (89.83%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW TKTS CF FPYP FVS T+RL P L CSSS TASTDA STQCAP
Sbjct: 1 MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
NYTIRSKYRTRI+ +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300
Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
PSLS C EN EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360
Query: 361 FGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSL 420
GYDFVEISIWGESFMLHQ IRKM+GTAIAVKRKLLPRDIIALSL
Sbjct: 361 LGYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSL 420
Query: 421 TKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILP 480
+KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLN+SDDIVKSVNEFYRS+ILP
Sbjct: 421 SKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILP 480
Query: 481 EVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
EVTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 EVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 505
BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match:
O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)
HSP 1 Score: 493.0 bits (1268), Expect = 4.1e-138
Identity = 266/527 (50.47%), Postives = 354/527 (67.17%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTN--RLWPSLPSCSSSPTASTDAFSTQC 60
M VSFLR+P+ P F PT R+ P+L SSS A+ ST
Sbjct: 1 MAVSFLRIPI--------------PSRVFWYPTRLIRVTPALLRFSSS--AAFQPSSTSL 60
Query: 61 APDQ-----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGG 120
+P KWE++RKKKVV+RIGYVGTDYRGLQ+QR+ S TIE ELE AIYKAGG
Sbjct: 61 SPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGG 120
Query: 121 IRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLP 180
IR+SNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW DP G LA I+ +LP
Sbjct: 121 IRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLP 180
Query: 181 DNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSF 240
+NI+VF ++PS R FDPR+EC +RKYSYLLP ++GI++ + E+D HI+DF +IL F
Sbjct: 181 ENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEF 240
Query: 241 QGEHPFHNYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVK 300
+GE+PFHNYT RS+YR + + K K N P + + + ES+ ++ +E +
Sbjct: 241 EGEYPFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGE 300
Query: 301 EPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSC 360
E +++ V E+ V + D S++ RA+WL+EPDE D+I +HFRK+ C
Sbjct: 301 EEEEK----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRS 360
Query: 361 GKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRD 420
GKLE S G+ FVEISIWGESFMLHQ IRKMIGTA+AVKR+LLPRD
Sbjct: 361 GKLENSLGFGFVEISIWGESFMLHQ----------------IRKMIGTAVAVKRELLPRD 420
Query: 421 IIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFY 480
II LSL KF+RIVLP+AP+EVL+LRGNSF R+ +P+RP + ES+++ K + EFY
Sbjct: 421 IIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFY 480
Query: 481 RSSILPEVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
R+ ++P+V+ FLD+E+ PWKEWV+ LD+ + +E+L++VR W+ W
Sbjct: 481 RAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEW 480
BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match:
Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)
HSP 1 Score: 99.0 bits (245), Expect = 1.7e-19
Identity = 110/423 (26.00%), Postives = 188/423 (44.44%), Query Frame = 0
Query: 71 KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
K+KV IGY GT Y G+Q+ Q+ TIE ++ A KAG I ++N + K ++ R+
Sbjct: 84 KRKVACMIGYCGTGYHGMQLNPPQK---TIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 143
Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
+RTDKGVH+ I+LKM I + ++ IN +LP+ ++V+G+ + ++F+
Sbjct: 144 ARTDKGVHAAGNVISLKMIIEDE---------NIVEKINSHLPEQLRVWGVSRTNKAFEC 203
Query: 191 RKECDVRKYSYLLPA--------KLIGIESKLK--TTEVDCHISDFKDILNSFQGEHPFH 250
RK C R Y YL+P + E LK T+ + ++ + + + +
Sbjct: 204 RKLCSSRVYEYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEEGKKYWESVAADLESQGVSY 263
Query: 251 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 310
+ ++ I+ G E +V+ SE + D +E +K ++R
Sbjct: 264 DEWMKRACIDEIK-----------GEET--KEVAESEVKTDSKTDAATLEKIKAVERRHR 323
Query: 311 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 370
+ E +K+ + ++ +G + + K S R + +C
Sbjct: 324 EE---FRISGERLAKIREILKIYEGTH-NFHNFTLGKAFKDPSAMRTMKSLTCSDPFLID 383
Query: 371 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIG-TAIAVKRKLLPRDIIALSL 430
G ++V I I G+SFMLHQ IRKMI A++V+ P+ +I +
Sbjct: 384 GTEWVSIKIHGQSFMLHQ----------------IRKMISMVALSVRCNADPQKLIPQTF 443
Query: 431 TKFSRIVLPIAPAEVLVLRGNSFS--NRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSI 481
K +RI +P APA L+L + N+K G R + N +D I ++F I
Sbjct: 444 EK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIYDKI 460
BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match:
Q755C8 (tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PUS1 PE=3 SV=2)
HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 91/321 (28.35%), Postives = 144/321 (44.86%), Query Frame = 0
Query: 71 KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
K+KV + +GY GT Y G+Q + TIE EL A KAG I +N +L K + R+
Sbjct: 66 KRKVAVMVGYCGTGYHGMQYNPPNR---TIEAELFEAFVKAGAISRANSTDLKKNGFMRA 125
Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
+RTDKGVH+ I+LK+ I + A + + IN +LP I+V+GI ++FD
Sbjct: 126 ARTDKGVHAGGNVISLKLIIEDPA---------IKDKINEHLPPGIRVWGISRVNKAFDC 185
Query: 191 RKECDVRKYSYLLPA-KLIGIE--SKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSK 250
RK C R Y YLLP IG + + L T C + S + + +
Sbjct: 186 RKLCGSRWYEYLLPTYSFIGPKPNTYLARTIEQCGEA------ASEKPDRDQESLDFWEA 245
Query: 251 YRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYE 310
+R + K + N V+P S +F+E+ G + VK+ Q + Y
Sbjct: 246 FRKAVDEKFTQEEQDAIVNYVAP---SKEDFDENSG----LYQKVKQYKQMENAHRRSYR 305
Query: 311 NPTEVCSKLGDGSELPKGVR--ARWLHEPDEKDRIGASHFRKILYCS---CGKLETSFGY 370
+ ++ + + G + D KD + I G+++T
Sbjct: 306 VSSAKLARFREAMKQYLGPHNFHNYTLGKDFKDPSTVRFMKDITVSDPFVIGEMKT---- 357
Query: 371 DFVEISIWGESFMLHQLAALL 384
++V I I G+SFMLHQ+ ++
Sbjct: 366 EWVSIKIHGQSFMLHQIRKMI 357
BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match:
Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)
HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 55/180 (30.56%), Postives = 94/180 (52.22%), Query Frame = 0
Query: 71 KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
K+K+VL + Y G Y G+Q TIE +L +A+ +AG I E++ ++ K+S+ R
Sbjct: 79 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 138
Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
+RTDKGV + ++LK+ W+ D + + IN +LP +I++ G+ F+
Sbjct: 139 ARTDKGVSAAGQVVSLKV------WLID----DILDKINSHLPSHIRILGLKRVTGGFNS 198
Query: 191 RKECDVRKYSYLLPA-------KLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYT 244
+ +CD R Y Y+LP + + ES + E + +L ++G H FHN+T
Sbjct: 199 KNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAET---LQQVNRLLGCYKGTHNFHNFT 245
BLAST of CcUC03G042520 vs. ExPASy Swiss-Prot
Match:
Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)
HSP 1 Score: 94.4 bits (233), Expect = 4.2e-18
Identity = 55/180 (30.56%), Postives = 94/180 (52.22%), Query Frame = 0
Query: 71 KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
K+K+VL + Y G Y G+Q TIE +L +A+ +AG I E++ ++ K+S+ R
Sbjct: 79 KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 138
Query: 131 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 190
+RTDKGV + ++LK+ W+ D + + IN +LP +I++ G+ F+
Sbjct: 139 ARTDKGVSAAGQVVSLKV------WLID----DILDKINSHLPSHIRILGLKRVTGGFNS 198
Query: 191 RKECDVRKYSYLLPA-------KLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYT 244
+ +CD R Y Y+LP + + ES + E + +L ++G H FHN+T
Sbjct: 199 KNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAET---LQQVNRLLACYKGTHNFHNFT 245
BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match:
A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)
HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 453/525 (86.29%), Postives = 475/525 (90.48%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M+VS LRLPLSPW TK+SSSCF FPYP FV T RL P+L C SS ASTDAFSTQCAP
Sbjct: 1 MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRS YRTRI+ K+SPG+A KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+IL CSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDF+EISIWGESFMLHQ IRKMIGTA+AVKRKLLPRD+IALSLT
Sbjct: 361 GYDFIEISIWGESFMLHQ----------------IRKMIGTAVAVKRKLLPRDVIALSLT 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGNSFS RKRPGVP RPDL+TLNESDDI+KSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAWNCRVH 526
VT FLDTE PPWKEWVEKLDKYTSIPNE+LDEVR+AWKAW RVH
Sbjct: 481 VTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508
BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match:
A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)
HSP 1 Score: 892.5 bits (2305), Expect = 8.5e-256
Identity = 444/520 (85.38%), Postives = 468/520 (90.00%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW KTS CF FPYP FVS T+RL P L CSSS TASTDA STQCAP
Sbjct: 1 MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
+LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYRTRI+T +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
SLS YEN EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKM+GTAIAVKRKLLPRDIIALSL+
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSLS 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLNES DIVKSVNEFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
VTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 504
BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match:
A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)
HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 444/521 (85.22%), Postives = 468/521 (89.83%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW TKTS CF FPYP FVS T+RL P L CSSS TASTDA STQCAP
Sbjct: 1 MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
NYTIRSKYRTRI+ +SPG+ HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300
Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETS 360
PSLS C EN EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKIL CSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360
Query: 361 FGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSL 420
GYDFVEISIWGESFMLHQ IRKM+GTAIAVKRKLLPRDIIALSL
Sbjct: 361 LGYDFVEISIWGESFMLHQ----------------IRKMVGTAIAVKRKLLPRDIIALSL 420
Query: 421 TKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILP 480
+KFSRIVLPIAPAEVLVLRGN FSNRKRPG PKRPDLLTLN+SDDIVKSVNEFYRS+ILP
Sbjct: 421 SKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILP 480
Query: 481 EVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
EVTNFLD ERPPWKEWVEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 EVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 505
BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match:
A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)
HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/520 (82.50%), Postives = 459/520 (88.27%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW TKTSS+CF PY AFVS + L P L C SSP+ASTDA ST+CAP
Sbjct: 1 MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61 DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYR I+ ++SPG+ H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKM+GTA+AVKRKLLPRDIIALSLT
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAVAVKRKLLPRDIIALSLT 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGNSFSN KRPG PKRPDLLTLN+SDDI+KSV+EFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
+TNFLDTE+ PW EWVEKLDKYTSIPNEQLDEVRNAWKAW
Sbjct: 481 LTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAW 503
BLAST of CcUC03G042520 vs. ExPASy TrEMBL
Match:
A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)
HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/520 (82.50%), Postives = 459/520 (88.27%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTNRLWPSLPSCSSSPTASTDAFSTQCAP 60
M VS LR PLSPW TKTSS+CF PY AFVS + L P L C SSP+ASTDA ST+CAP
Sbjct: 1 MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
Query: 61 DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61 DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
Query: 241 NYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
NYTIRSKYR I+ ++SPG+ H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSF 360
LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
Query: 361 GYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLT 420
GYDFVEISIWGESFMLHQ IRKM+GTA+AVKRKLLPRDIIALSLT
Sbjct: 361 GYDFVEISIWGESFMLHQ----------------IRKMVGTAVAVKRKLLPRDIIALSLT 420
Query: 421 KFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSSILPE 480
KFSRIVLPIAPAEVLVLRGNSFSN KRPG PKRPDLLTLN+SDDI+KSV+EFYRS+ILPE
Sbjct: 421 KFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPE 480
Query: 481 VTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
+TNFLDTE+ PW EWVEKLDKYTSIPNEQLDEVRNAWKAW
Sbjct: 481 LTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAW 503
BLAST of CcUC03G042520 vs. TAIR 10
Match:
AT2G30320.1 (Pseudouridine synthase family protein )
HSP 1 Score: 493.0 bits (1268), Expect = 2.9e-139
Identity = 266/527 (50.47%), Postives = 354/527 (67.17%), Query Frame = 0
Query: 1 MTVSFLRLPLSPWTTKTSSSCFPFPYPAFVSPTN--RLWPSLPSCSSSPTASTDAFSTQC 60
M VSFLR+P+ P F PT R+ P+L SSS A+ ST
Sbjct: 1 MAVSFLRIPI--------------PSRVFWYPTRLIRVTPALLRFSSS--AAFQPSSTSL 60
Query: 61 APDQ-----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGG 120
+P KWE++RKKKVV+RIGYVGTDYRGLQ+QR+ S TIE ELE AIYKAGG
Sbjct: 61 SPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGG 120
Query: 121 IRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLP 180
IR+SNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW DP G LA I+ +LP
Sbjct: 121 IRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLP 180
Query: 181 DNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSF 240
+NI+VF ++PS R FDPR+EC +RKYSYLLP ++GI++ + E+D HI+DF +IL F
Sbjct: 181 ENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEF 240
Query: 241 QGEHPFHNYTIRSKYRTRIQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVK 300
+GE+PFHNYT RS+YR + + K K N P + + + ES+ ++ +E +
Sbjct: 241 EGEYPFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGE 300
Query: 301 EPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILYCSC 360
E +++ V E+ V + D S++ RA+WL+EPDE D+I +HFRK+ C
Sbjct: 301 EEEEK----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRS 360
Query: 361 GKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRKLLPRD 420
GKLE S G+ FVEISIWGESFMLHQ IRKMIGTA+AVKR+LLPRD
Sbjct: 361 GKLENSLGFGFVEISIWGESFMLHQ----------------IRKMIGTAVAVKRELLPRD 420
Query: 421 IIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFY 480
II LSL KF+RIVLP+AP+EVL+LRGNSF R+ +P+RP + ES+++ K + EFY
Sbjct: 421 IIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFY 480
Query: 481 RSSILPEVTNFLDTERPPWKEWVEKLDKYTSIPNEQLDEVRNAWKAW 521
R+ ++P+V+ FLD+E+ PWKEWV+ LD+ + +E+L++VR W+ W
Sbjct: 481 RAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEW 480
BLAST of CcUC03G042520 vs. TAIR 10
Match:
AT1G20370.1 (Pseudouridine synthase family protein )
HSP 1 Score: 111.7 bits (278), Expect = 1.8e-24
Identity = 111/412 (26.94%), Postives = 176/412 (42.72%), Query Frame = 0
Query: 64 RKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLN 123
R+ +R++KV + + G Y+G+Q + TIE ELE A++ AG + +++
Sbjct: 50 RRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPDADRNKPR 109
Query: 124 KISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVP 183
WARS+RTDKGV ++ ++ + + DP G +N LPD I+VFG
Sbjct: 110 NYEWARSARTDKGVSAVGQVVSGRFYV-------DPPG--FVERLNSKLPDQIRVFGYKR 169
Query: 184 SQRSFDPRKECDVRKYSYLLPAKLIG--IESKLKTTEVDCHISDFKDILNSFQG-EHPF- 243
SF +K CD R+Y YL+P + + + + D K + S +G + P
Sbjct: 170 VAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTDLGYEYVKCVECSEKGYKIPLG 229
Query: 244 ---HNYTIRSKYRTRIQTKRSPGHAHKSGNEVS--------PSDVSNSEFEESDGEDDTF 303
+ IQ+ S G +V PS +N E DG D +
Sbjct: 230 VMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCETLSSSVPSAENNLNSEVLDGADVSA 289
Query: 304 VEGVKEPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDR--------I 363
+ E + S +L+ +V G+G ++ K +++ + EK+R +
Sbjct: 290 LSSKAEGMEESNTLA-------KVEMNNGEGEDMTKS-ESKFCYGEKEKERFNRILSYYV 349
Query: 364 GASHF---------------RKILYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLR 423
G+ +F R IL + + G DFV+ + G+SFMLHQ
Sbjct: 350 GSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQ------- 409
Query: 424 FGFPILTPLIRKMIGTAIAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 438
IRKMIG A+A+ R P +I S K RI +P+AP L L
Sbjct: 410 ---------IRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLYL 425
BLAST of CcUC03G042520 vs. TAIR 10
Match:
AT1G76120.1 (Pseudouridine synthase family protein )
HSP 1 Score: 98.6 bits (244), Expect = 1.6e-20
Identity = 102/399 (25.56%), Postives = 168/399 (42.11%), Query Frame = 0
Query: 72 KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
+KV + + G Y+G+Q + TIE ELE A++ AG + ES G +ARS+
Sbjct: 22 QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81
Query: 132 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 191
RTDKGV ++ ++ ++ DP G N +N LP+ I++FG SF +
Sbjct: 82 RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141
Query: 192 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTR 251
K CD R+Y YLLP + S V + ++ + F+ R +
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201
Query: 252 IQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 311
+ G + + SD+S++ S D +E + S+ V E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261
Query: 312 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 371
KL S K V++++ + EK+R +G+ +F R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321
Query: 372 LYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRK 431
+ + + G DF++ + G+SFMLHQ IRKM+G A+A+ R
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQ----------------IRKMMGLAVAIMRN 381
Query: 432 LLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKR 448
+I + +K I +P+AP L L F++ R
Sbjct: 382 CASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNR 382
BLAST of CcUC03G042520 vs. TAIR 10
Match:
AT1G76120.2 (Pseudouridine synthase family protein )
HSP 1 Score: 90.1 bits (222), Expect = 5.6e-18
Identity = 94/373 (25.20%), Postives = 156/373 (41.82%), Query Frame = 0
Query: 72 KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 131
+KV + + G Y+G+Q + TIE ELE A++ AG + ES G +ARS+
Sbjct: 22 QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81
Query: 132 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 191
RTDKGV ++ ++ ++ DP G N +N LP+ I++FG SF +
Sbjct: 82 RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141
Query: 192 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTR 251
K CD R+Y YLLP + S V + ++ + F+ R +
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201
Query: 252 IQTKRSPGHAHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 311
+ G + + SD+S++ S D +E + S+ V E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261
Query: 312 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 371
KL S K V++++ + EK+R +G+ +F R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321
Query: 372 LYCSCGKLETSFGYDFVEISIWGESFMLHQLAALLLRFGFPILTPLIRKMIGTAIAVKRK 422
+ + + G DF++ + G+SFMLHQ IRKM+G A+A+ R
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQ----------------IRKMMGLAVAIMRN 356
BLAST of CcUC03G042520 vs. TAIR 10
Match:
AT1G34150.1 (Pseudouridine synthase family protein )
HSP 1 Score: 54.3 bits (129), Expect = 3.4e-07
Identity = 57/225 (25.33%), Postives = 86/225 (38.22%), Query Frame = 0
Query: 71 KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 130
K+ V L+I Y G + G E Q P+IE E ++KA G+ ++R
Sbjct: 89 KRYVALKIMYFGKRFYG--FSAEAQMEPSIESE----VFKALERTRLLVGDKKDSCYSRC 148
Query: 131 SRTDKGVHSLATTITL----KMEIPE-------SAWMGDPCGISLANYINFYLPDNIKVF 190
RTDKGV S I L +++ P + G+ +N LPD+I+V
Sbjct: 149 GRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVI 208
Query: 191 GIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPF 250
G P+ F R C R+Y Y + + + + DF F GEH F
Sbjct: 209 GWSPAPIDFHARFSCYAREYKYFFWRQNLNLSA-----------MDFAG--KKFIGEHDF 268
Query: 251 HNYTIRSKYRTRIQTKR------SPGHAHKSGNEVSPSDVSNSEF 279
N+ T+R SP G+++ + S F
Sbjct: 269 RNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAF 294
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038894774.1 | 1.3e-271 | 90.10 | putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida] | [more] |
XP_008457308.1 | 5.8e-259 | 86.29 | PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo] | [more] |
XP_022964935.1 | 1.8e-255 | 85.38 | putative tRNA pseudouridine synthase [Cucurbita moschata] | [more] |
XP_023520301.1 | 8.7e-255 | 85.38 | putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo] | [more] |
XP_022970646.1 | 4.3e-254 | 85.22 | putative tRNA pseudouridine synthase [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
O22928 | 4.1e-138 | 50.47 | Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032... | [more] |
Q6CC39 | 1.7e-19 | 26.00 | tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O... | [more] |
Q755C8 | 2.5e-18 | 28.35 | tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... | [more] |
Q4KM92 | 2.5e-18 | 30.56 | tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2 | [more] |
Q9WU56 | 4.2e-18 | 30.56 | tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3C5W1 | 2.8e-259 | 86.29 | putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=... | [more] |
A0A6J1HM83 | 8.5e-256 | 85.38 | putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648... | [more] |
A0A6J1I156 | 2.1e-254 | 85.22 | putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569... | [more] |
A0A6J1DMV6 | 3.6e-246 | 82.50 | putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G... | [more] |
A0A6J1DQ07 | 3.6e-246 | 82.50 | putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G... | [more] |