CcUC03G042510 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC03G042510
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationCicolChr03: 229755 .. 232268 (+)
RNA-Seq ExpressionCcUC03G042510
SyntenyCcUC03G042510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTCTTCCATAGTCTTCCAATGGCTTAGCCTCGTTGGAATAATATGGCTTCAATCCATCAATGGCACCAACTTCAATTTCCCCTCTTATTCTTCTCAGCTCAAACAACAACTTTCCATGTCACAATTACAACTCAACAACCTCGCATTCGCCTCCGACGCCGGCAAGCTCTTTGCTTGCTTCTCTGGCCTTGCTGCTAACTATTTACCTCTTTGGTTAGTCCTCATGATCGGTTCTTCTCTCGGCCTTGTTGGCTATGGCGTTCAATATCTCTTCATTACCAATCAATTCCAATCTCCTTCTTATTGGGTCATCTTCTCCCTCACTGTTCTAGCCGGAAACAGTATTTGTTGGATCAACACCGTTTGTTACATGGTTGCCATTACAAACTTCACATCGTCGAATCGCCAAGTTGCGGTTGGGATATCAACAAGTTACCAAGGATTAAGCGCCAAGGTTTATACGAATATTGTCGGTTCAATATTCACTAAACACTCTTCCAAAACAGCTGAAATATTTCTTCTATTGAACTCTGTTTTGCCTTTGGGGGTTTGTGTCTTGGCATCTCCGTTGGCTAGAGTTGTGAAAGTAGTGGAAGAACAAGGAAAATTGGAAGTTGGGTTTATTGTAATCTTTGTTATAACCATAGCCACTGGGGTTTTTGCAGCAGTTACAAGCGTGGGATCGATTTCAAGAATGTTGTCGCCATTGAGTGGAGTGATTGGGATTTTGGTGTTTCTGCTTCTTCCGTTAGTGGTGGCAGTTGTAGAGAAAGTGAAGGAGTCAGTAAAAGAAGGAAAAGAGGGAAGGGTTTATCATATTACAGTGGACGAAAATAAAGATGTAGAGAGAATGAGAGGGGAAAATGAGATGAAATTGGAAAGAGAGGGAGATGGTGAAGCAATGGAGGAGATTGGGGCGAGAGAGATGTTGAAAAGAATAAATTTTTGGTTATATTTTGGTGTTTATTTATTTGGTGCAACACTGGGATTGGCGTTCTTGAACAACTTGGGGCAAATAGCAGAGTCTCGTGGAAGCTCGAGTGTGTCATCGTTGGTATCTTTGTCGTCATCGTTTGGGTTCTTCGGCCGCCTCATGCCCTCAATTCTCGACCATCTTCTGTCCAGGTAAATAAATTCAACGCTTAAATATTACTTTGCTTCATTTATCTCCTATCTTTTAGTTTATTTTGTTTTTATACTTTTGAAATATTTATTTAAATTCCTATATATATTTTATTTTTTTCTCTTTTTTCTTTTCATTTTGATTTCGAAAAATATATAATGTGTAATTAATGGTCATGTATCATAAAGTTTTAATTATTTAAAAAAATTAAATTTTTTTTTTCTATCTGATAAATGATAAATAATGTAAGTAAGGTTCTAACTAACTATTGTCATTTTGGTTCTTATACTTTTAAACTTTGATCCATTTTAGTCTTTCAATTCTAAAAAAATGACTAATTTGATGAAATAAAAATGAATATTAAGTGACTAAAATGATCACTTTTTAAAAGTGCAGGAATCAAAATGCTTTAAAGTTGAAAATAGACCAAAATAAATATTTTTAAAGTACAAATAATAAAATAAACAAAAACTAAAAGTATAGGAAACCAAGTAGTATTTAAATCTAAAATGTTGTAAAATGAGGCTTGTGAGACTATGTTTGAACTTGACTAATTTAATTTTACAAAAAGCAATGATTAGGTGCAGCTTAGGTTGCACCTAATCTTATGCTTCTCCTTTGTTTACCCACAAATTTCTTTTTTTTTCTTTTTTGAAATAAGGAAGTATATTTACTCATGATAATTTTTTACTAAACATTTGTCTAAGCAACACAAAGATAGTTGAGTGAAACAATATGTAAAATCTCATTTAGTAACCATTTGGTTTTTTGTTTTTTATTTTTGAAAGTTACTCCTATAGAGTCTACTGCAACTTCCAAATTTATTGTTTTGTTATCTACATTTTAGCAATAATTGAAAAAATAAAGCCAATTTTGGAAAAAATGGAGCCAAGTTTTGAATGTTGATGCAGGAAGAAGTGTATGAAATCAAAGGCAGGTTGGATGGTGGGATTGATGGGGGCATTATGTGGAGGCTTCTTCCTTCTTTTAAGTCCAAGTGATGTGAGTTTGTGTGTAAGCACAGCCATAATTGCAATATGTACGGGTGCAATTACTTCCATTTCAGTGTCCATAACCAGTGAGTTATTTGGGGCTAACAATTTCTCAATAAACCACAACATTCTTGTTGCCAATATACCTTTTGGTTCATTTCTATTTGGCTATATGGCGGCTTTTCTTTACCACAAACAAGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGATGCCACTGGGAAATGCATTGGAGTTGAGTGCTATAAAACAACTTTTATGATATGGGGTTTCTTATGTTCCTTTGGGACTTTATTGGCTTTAATTTTGTTTGCTCGAACTAGAAGCTTCTACTCTCCAACAAACTCTCATATTCCACCACATTCTTCTACGTAG

mRNA sequence

ATGCCTTCTTCCATAGTCTTCCAATGGCTTAGCCTCGTTGGAATAATATGGCTTCAATCCATCAATGGCACCAACTTCAATTTCCCCTCTTATTCTTCTCAGCTCAAACAACAACTTTCCATGTCACAATTACAACTCAACAACCTCGCATTCGCCTCCGACGCCGGCAAGCTCTTTGCTTGCTTCTCTGGCCTTGCTGCTAACTATTTACCTCTTTGGTTAGTCCTCATGATCGGTTCTTCTCTCGGCCTTGTTGGCTATGGCGTTCAATATCTCTTCATTACCAATCAATTCCAATCTCCTTCTTATTGGGTCATCTTCTCCCTCACTGTTCTAGCCGGAAACAGTATTTGTTGGATCAACACCGTTTGTTACATGGTTGCCATTACAAACTTCACATCGTCGAATCGCCAAGTTGCGGTTGGGATATCAACAAGTTACCAAGGATTAAGCGCCAAGGTTTATACGAATATTGTCGGTTCAATATTCACTAAACACTCTTCCAAAACAGCTGAAATATTTCTTCTATTGAACTCTGTTTTGCCTTTGGGGGTTTGTGTCTTGGCATCTCCGTTGGCTAGAGTTGTGAAAGTAGTGGAAGAACAAGGAAAATTGGAAGTTGGGTTTATTGTAATCTTTGTTATAACCATAGCCACTGGGGTTTTTGCAGCAGTTACAAGCGTGGGATCGATTTCAAGAATGTTGTCGCCATTGAGTGGAGTGATTGGGATTTTGGTGTTTCTGCTTCTTCCGTTAGTGGTGGCAGTTGTAGAGAAAGTGAAGGAGTCAGTAAAAGAAGGAAAAGAGGGAAGGGTTTATCATATTACAGTGGACGAAAATAAAGATGTAGAGAGAATGAGAGGGGAAAATGAGATGAAATTGGAAAGAGAGGGAGATGGTGAAGCAATGGAGGAGATTGGGGCGAGAGAGATGTTGAAAAGAATAAATTTTTGGTTATATTTTGGTGTTTATTTATTTGGTGCAACACTGGGATTGGCGTTCTTGAACAACTTGGGGCAAATAGCAGAGTCTCGTGGAAGCTCGAGTGTGTCATCGTTGGTATCTTTGTCGTCATCGTTTGGGTTCTTCGGCCGCCTCATGCCCTCAATTCTCGACCATCTTCTGTCCAGGAAGAAGTGTATGAAATCAAAGGCAGGTTGGATGGTGGGATTGATGGGGGCATTATGTGGAGGCTTCTTCCTTCTTTTAAGTCCAAGTGATGTGAGTTTGTGTGTAAGCACAGCCATAATTGCAATATGTACGGGTGCAATTACTTCCATTTCAGTGTCCATAACCAGTGAGTTATTTGGGGCTAACAATTTCTCAATAAACCACAACATTCTTGTTGCCAATATACCTTTTGGTTCATTTCTATTTGGCTATATGGCGGCTTTTCTTTACCACAAACAAGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGATGCCACTGGGAAATGCATTGGAGTTGAGTGCTATAAAACAACTTTTATGATATGGGGTTTCTTATGTTCCTTTGGGACTTTATTGGCTTTAATTTTGTTTGCTCGAACTAGAAGCTTCTACTCTCCAACAAACTCTCATATTCCACCACATTCTTCTACGTAG

Coding sequence (CDS)

ATGCCTTCTTCCATAGTCTTCCAATGGCTTAGCCTCGTTGGAATAATATGGCTTCAATCCATCAATGGCACCAACTTCAATTTCCCCTCTTATTCTTCTCAGCTCAAACAACAACTTTCCATGTCACAATTACAACTCAACAACCTCGCATTCGCCTCCGACGCCGGCAAGCTCTTTGCTTGCTTCTCTGGCCTTGCTGCTAACTATTTACCTCTTTGGTTAGTCCTCATGATCGGTTCTTCTCTCGGCCTTGTTGGCTATGGCGTTCAATATCTCTTCATTACCAATCAATTCCAATCTCCTTCTTATTGGGTCATCTTCTCCCTCACTGTTCTAGCCGGAAACAGTATTTGTTGGATCAACACCGTTTGTTACATGGTTGCCATTACAAACTTCACATCGTCGAATCGCCAAGTTGCGGTTGGGATATCAACAAGTTACCAAGGATTAAGCGCCAAGGTTTATACGAATATTGTCGGTTCAATATTCACTAAACACTCTTCCAAAACAGCTGAAATATTTCTTCTATTGAACTCTGTTTTGCCTTTGGGGGTTTGTGTCTTGGCATCTCCGTTGGCTAGAGTTGTGAAAGTAGTGGAAGAACAAGGAAAATTGGAAGTTGGGTTTATTGTAATCTTTGTTATAACCATAGCCACTGGGGTTTTTGCAGCAGTTACAAGCGTGGGATCGATTTCAAGAATGTTGTCGCCATTGAGTGGAGTGATTGGGATTTTGGTGTTTCTGCTTCTTCCGTTAGTGGTGGCAGTTGTAGAGAAAGTGAAGGAGTCAGTAAAAGAAGGAAAAGAGGGAAGGGTTTATCATATTACAGTGGACGAAAATAAAGATGTAGAGAGAATGAGAGGGGAAAATGAGATGAAATTGGAAAGAGAGGGAGATGGTGAAGCAATGGAGGAGATTGGGGCGAGAGAGATGTTGAAAAGAATAAATTTTTGGTTATATTTTGGTGTTTATTTATTTGGTGCAACACTGGGATTGGCGTTCTTGAACAACTTGGGGCAAATAGCAGAGTCTCGTGGAAGCTCGAGTGTGTCATCGTTGGTATCTTTGTCGTCATCGTTTGGGTTCTTCGGCCGCCTCATGCCCTCAATTCTCGACCATCTTCTGTCCAGGAAGAAGTGTATGAAATCAAAGGCAGGTTGGATGGTGGGATTGATGGGGGCATTATGTGGAGGCTTCTTCCTTCTTTTAAGTCCAAGTGATGTGAGTTTGTGTGTAAGCACAGCCATAATTGCAATATGTACGGGTGCAATTACTTCCATTTCAGTGTCCATAACCAGTGAGTTATTTGGGGCTAACAATTTCTCAATAAACCACAACATTCTTGTTGCCAATATACCTTTTGGTTCATTTCTATTTGGCTATATGGCGGCTTTTCTTTACCACAAACAAGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGATGCCACTGGGAAATGCATTGGAGTTGAGTGCTATAAAACAACTTTTATGATATGGGGTTTCTTATGTTCCTTTGGGACTTTATTGGCTTTAATTTTGTTTGCTCGAACTAGAAGCTTCTACTCTCCAACAAACTCTCATATTCCACCACATTCTTCTACGTAG

Protein sequence

MPSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGVIGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDGEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAICTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPTNSHIPPHSST
Homology
BLAST of CcUC03G042510 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 869.8 bits (2246), Expect = 1.2e-248
Identity = 462/528 (87.50%), Postives = 491/528 (92.99%), Query Frame = 0

Query: 1   MPSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA 60
           M SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA
Sbjct: 1   MSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA 60

Query: 61  CFSGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWI 120
           CFSGLAANYLPLWLVL IG+SLGLVGYGVQYLF+TNQ QSPSY VIF LTVLAGNSICWI
Sbjct: 61  CFSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWI 120

Query: 121 NTVCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSV 180
           NTVCYMVAITNF  +NRQ+AVGISTSYQGLSAK+YTNIV SIF+KHSSKT E FLLLNSV
Sbjct: 121 NTVCYMVAITNF-GTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSV 180

Query: 181 LPLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSG 240
           LP+GVCVLASPLAR++KVVEEQGKLEVGF VIFVITIATG+FAAVTSVGSISRMLSPLSG
Sbjct: 181 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 240

Query: 241 VIGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDG 300
           ++GI+VFL+LPLVVAVVEKVKES+KEGKE RVYH TV+ENKDVER+RGEN+ KLER+GD 
Sbjct: 241 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDS 300

Query: 301 -EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 360
            EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 301 EEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 360

Query: 361 FGFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 420
           FGFFGRLMPSILD+ LSRKKCMKSK GWMVGLMG LCGGFFLLL P+DVSLCVSTAIIA+
Sbjct: 361 FGFFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAV 420

Query: 421 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 480
           CTGAITSISVS TSELFGANNFSINHN+LVANIPFGSFLFGYMAAFLYHKQ       A 
Sbjct: 421 CTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQ-------AG 480

Query: 481 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPTNSH 528
            D TGKCIGVECY+TTF IWG   SFGTLLAL+LFART++FYS T SH
Sbjct: 481 GDGTGKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTRSH 520

BLAST of CcUC03G042510 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 844.3 bits (2180), Expect = 5.6e-241
Identity = 455/534 (85.21%), Postives = 487/534 (91.20%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 4   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 63

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 64  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 123

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SSNRQVAVGISTSYQGLSAKVYT+IVGSIF+ K SSKTAE FLLLNSVL
Sbjct: 124 VCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVL 183

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG FA +TSVGS+SRMLS LSG+
Sbjct: 184 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 243

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVER-MRGENEMKLEREGDG 302
           +GI+VFL+LPLVV V EKVKE V+EGKEGRVYH TV+EN DVER MRGENE K+ER  +G
Sbjct: 244 VGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 422
           GFFGRL+PSILD+ LSR+ K MKSK GWMVGLMGALCGGFFLLLSPSD SLC+STAIIAI
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIAI 423

Query: 423 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 482
           CTGAITSISVS T++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    AA  
Sbjct: 424 CTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA-- 483

Query: 483 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIPPH 532
               GKCIGVECY+TTF+IWG   SFGT LAL+LFARTRSFYS T  +SHI P+
Sbjct: 484 ----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of CcUC03G042510 vs. NCBI nr
Match: XP_011658866.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN65862.1 hypothetical protein Csa_023247 [Cucumis sativus])

HSP 1 Score: 832.0 bits (2148), Expect = 2.9e-237
Identity = 447/531 (84.18%), Postives = 481/531 (90.58%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 5   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 64

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 65  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 124

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SS+RQVAVGISTSYQGLSAKVYT+IVGSIF+ KHSSKTAE FLLLNSVL
Sbjct: 125 VCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVL 184

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG+FA +TSVGS+SRMLS L G+
Sbjct: 185 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGGL 244

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDGE 302
           +GI+VFL+LPLVV VVEKVKE V+EGKEG+VYH TV+E  D ERMRGENE K+ER  DGE
Sbjct: 245 VGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDGE 304

Query: 303 AMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 362
           AMEEIGA+EM+KRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG
Sbjct: 305 AMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 364

Query: 363 FFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAIC 422
           FFGRL+PSILD+ LSR+ K MKSK GWMVGLMG LCGGFFLLLSPSD SLC+STAIIAIC
Sbjct: 365 FFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIAIC 424

Query: 423 TGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAA 482
           TGAITSISVS T++LFGA NFSINHNI+VANIPFGSF+FGYMAAFLY KQA         
Sbjct: 425 TGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV----- 484

Query: 483 DATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIP 530
              GKCIGVECY+TTF+IWG   SFGT LAL+LFART+SFYS T  +S+IP
Sbjct: 485 -DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIP 529

BLAST of CcUC03G042510 vs. NCBI nr
Match: KAA0059993.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ97252.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 790.8 bits (2041), Expect = 7.3e-225
Identity = 433/534 (81.09%), Postives = 464/534 (86.89%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 4   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 63

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 64  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 123

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SSNRQVAVGISTSYQGLSAKVYT+IVGSIF+ K SSKTAE FLLLNSVL
Sbjct: 124 VCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVL 183

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG FA +TSVGS+SRMLS LSG+
Sbjct: 184 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 243

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVER-MRGENEMKLEREGDG 302
           +GI+VFL+LPLVV V EKVKE V+EGKEGRVYH TV+EN DVER MRGENE K+ER  +G
Sbjct: 244 VGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 422
           GFFGRL+PSILD+ LSR+ K MKSK GWMVGLMGALCG                      
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG---------------------- 423

Query: 423 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 482
             GAITSISVS T++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    AA  
Sbjct: 424 --GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA-- 483

Query: 483 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIPPH 532
               GKCIGVECY+TTF+IWG   SFGT LAL+LFARTRSFYS T  +SHI P+
Sbjct: 484 ----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of CcUC03G042510 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 790.4 bits (2040), Expect = 9.6e-225
Identity = 427/526 (81.18%), Postives = 463/526 (88.02%), Query Frame = 0

Query: 1   MPSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA 60
           MPSS VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLFA
Sbjct: 1   MPSS-VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFA 60

Query: 61  CFSGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWI 120
           C SGLAANYLPLW VL+IGSSLGLVGYGVQYLFITNQF SPSY +IF LTVLAGNSICWI
Sbjct: 61  CVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWI 120

Query: 121 NTVCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSV 180
           NTVCY+VAI NF  SNRQVAVGISTSYQGLSAK+YT+IVG IF+K S KTAE FLL NSV
Sbjct: 121 NTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 180

Query: 181 LPLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSG 240
            PLGVCVL SP+AR+V++VEEQG LEVGF VIFVITIATGVFA VTSVGSISRMLSPLSG
Sbjct: 181 FPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 240

Query: 241 VIGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDG 300
           ++GILVFLLLP+VV VVE VK+SV+EGKE RVYH T++E+    R+R ENE KL R+G  
Sbjct: 241 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 300

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSF
Sbjct: 301 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSF 360

Query: 361 GFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAIC 420
           GFFGRLMPS+LDH LSR+K MKSK GW+V LMGA+CGGFFLL  P+D+ LCVSTA+IA+C
Sbjct: 361 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVC 420

Query: 421 TGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAA 480
           TGAITSISVS TSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA   A     
Sbjct: 421 TGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA----- 480

Query: 481 DATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPTNS 527
              GKCIGVECY+TTFMIWG LCSFGTLLAL+LFARTRSFYS T S
Sbjct: 481 ---GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKS 513

BLAST of CcUC03G042510 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.8e-72
Identity = 195/534 (36.52%), Postives = 299/534 (55.99%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 68  NYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMV 127
            Y PL +VL   +++G VGYGVQ+L ITN    P Y ++F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 128 AITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVC- 187
            I +F  +NR +A+ ++ S+ G+SA +Y+    +I    +  ++ ++LLLNS++PL V  
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVSF 222

Query: 188 -----VLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 247
                VL  P        + +      F ++ V+ + T     ++S  + S  L+     
Sbjct: 223 AALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----F 282

Query: 248 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYH-------ITVDENKD----VERMRGEN 307
           IG +V L+ PL   ++   ++        R+ H       + +DE K+    V    G  
Sbjct: 283 IGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYE 342

Query: 308 EMKLEREGDGEAM-EEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSS 367
            M   +EG+   + +E   R ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S
Sbjct: 343 HMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 368 VSSLVSLSSSFGFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLL--SPSD 427
            ++LV++ SSF FFGRL+ +  D +   K+   ++ GW    +      FFLL   S   
Sbjct: 403 -TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQ 462

Query: 428 VSLCVSTAIIAICTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLY 487
            +L  +TA+I + +G I + +VSITS+LFG N+  +NHNIL+ NIP GS L+GY+AA +Y
Sbjct: 463 TALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIY 522

Query: 488 HKQAAAAAAAAAADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFY 522
              A+       +D+   CIG +CY  TF+ WG L   G + +L L+ RT+  Y
Sbjct: 523 EANASPDITPIVSDSI-VCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CcUC03G042510 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.7e-241
Identity = 455/534 (85.21%), Postives = 487/534 (91.20%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 4   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 63

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 64  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 123

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SSNRQVAVGISTSYQGLSAKVYT+IVGSIF+ K SSKTAE FLLLNSVL
Sbjct: 124 VCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVL 183

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG FA +TSVGS+SRMLS LSG+
Sbjct: 184 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 243

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVER-MRGENEMKLEREGDG 302
           +GI+VFL+LPLVV V EKVKE V+EGKEGRVYH TV+EN DVER MRGENE K+ER  +G
Sbjct: 244 VGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 422
           GFFGRL+PSILD+ LSR+ K MKSK GWMVGLMGALCGGFFLLLSPSD SLC+STAIIAI
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIAI 423

Query: 423 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 482
           CTGAITSISVS T++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    AA  
Sbjct: 424 CTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA-- 483

Query: 483 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIPPH 532
               GKCIGVECY+TTF+IWG   SFGT LAL+LFARTRSFYS T  +SHI P+
Sbjct: 484 ----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of CcUC03G042510 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.4e-237
Identity = 447/531 (84.18%), Postives = 481/531 (90.58%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 5   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 64

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 65  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 124

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SS+RQVAVGISTSYQGLSAKVYT+IVGSIF+ KHSSKTAE FLLLNSVL
Sbjct: 125 VCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVL 184

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG+FA +TSVGS+SRMLS L G+
Sbjct: 185 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGGL 244

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDGE 302
           +GI+VFL+LPLVV VVEKVKE V+EGKEG+VYH TV+E  D ERMRGENE K+ER  DGE
Sbjct: 245 VGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDGE 304

Query: 303 AMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 362
           AMEEIGA+EM+KRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG
Sbjct: 305 AMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 364

Query: 363 FFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAIC 422
           FFGRL+PSILD+ LSR+ K MKSK GWMVGLMG LCGGFFLLLSPSD SLC+STAIIAIC
Sbjct: 365 FFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIAIC 424

Query: 423 TGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAA 482
           TGAITSISVS T++LFGA NFSINHNI+VANIPFGSF+FGYMAAFLY KQA         
Sbjct: 425 TGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV----- 484

Query: 483 DATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIP 530
              GKCIGVECY+TTF+IWG   SFGT LAL+LFART+SFYS T  +S+IP
Sbjct: 485 -DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIP 529

BLAST of CcUC03G042510 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.5e-225
Identity = 433/534 (81.09%), Postives = 464/534 (86.89%), Query Frame = 0

Query: 3   SSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 62
           SSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF
Sbjct: 4   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 63

Query: 63  SGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINT 122
           SGLAANYLPLWLVL IGSSLGL+GYGVQYLFITNQF SPSYW+IF LTVLAGNSICWINT
Sbjct: 64  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 123

Query: 123 VCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFT-KHSSKTAEIFLLLNSVL 182
           VCYMVAITNF SSNRQVAVGISTSYQGLSAKVYT+IVGSIF+ K SSKTAE FLLLNSVL
Sbjct: 124 VCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVL 183

Query: 183 PLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 242
           PLGVCVL SPLAR VK+VEEQGKLEVGF VIFVITIATG FA +TSVGS+SRMLS LSG+
Sbjct: 184 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 243

Query: 243 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVER-MRGENEMKLEREGDG 302
           +GI+VFL+LPLVV V EKVKE V+EGKEGRVYH TV+EN DVER MRGENE K+ER  +G
Sbjct: 244 VGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHLLSRK-KCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 422
           GFFGRL+PSILD+ LSR+ K MKSK GWMVGLMGALCG                      
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG---------------------- 423

Query: 423 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 482
             GAITSISVS T++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    AA  
Sbjct: 424 --GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA-- 483

Query: 483 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPT--NSHIPPH 532
               GKCIGVECY+TTF+IWG   SFGT LAL+LFARTRSFYS T  +SHI P+
Sbjct: 484 ----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of CcUC03G042510 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.6e-225
Identity = 427/526 (81.18%), Postives = 463/526 (88.02%), Query Frame = 0

Query: 1   MPSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA 60
           MPSS VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLFA
Sbjct: 1   MPSS-VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFA 60

Query: 61  CFSGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWI 120
           C SGLAANYLPLW VL+IGSSLGLVGYGVQYLFITNQF SPSY +IF LTVLAGNSICWI
Sbjct: 61  CVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWI 120

Query: 121 NTVCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSV 180
           NTVCY+VAI NF  SNRQVAVGISTSYQGLSAK+YT+IVG IF+K S KTAE FLL NSV
Sbjct: 121 NTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 180

Query: 181 LPLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSG 240
            PLGVCVL SP+AR+V++VEEQG LEVGF VIFVITIATGVFA VTSVGSISRMLSPLSG
Sbjct: 181 FPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 240

Query: 241 VIGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDG 300
           ++GILVFLLLP+VV VVE VK+SV+EGKE RVYH T++E+    R+R ENE KL R+G  
Sbjct: 241 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 300

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSF
Sbjct: 301 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSF 360

Query: 361 GFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAIC 420
           GFFGRLMPS+LDH LSR+K MKSK GW+V LMGA+CGGFFLL  P+D+ LCVSTA+IA+C
Sbjct: 361 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVC 420

Query: 421 TGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAA 480
           TGAITSISVS TSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA   A     
Sbjct: 421 TGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA----- 480

Query: 481 DATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPTNS 527
              GKCIGVECY+TTFMIWG LCSFGTLLAL+LFARTRSFYS T S
Sbjct: 481 ---GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKS 513

BLAST of CcUC03G042510 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.5e-220
Identity = 421/527 (79.89%), Postives = 460/527 (87.29%), Query Frame = 0

Query: 1   MPSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFA 60
           MPSS VFQWLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKLFA
Sbjct: 55  MPSS-VFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFA 114

Query: 61  CFSGLAANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWI 120
           C SGLAANYLPLW +L+IGS LGLVGYGVQYLFITNQF SPSY +IF L+VLAGNSICWI
Sbjct: 115 CVSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWI 174

Query: 121 NTVCYMVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSV 180
           NTVCY+VAI NF  SNRQVAVGISTSYQGLSAK+YT+IVG IF+K S KTAE FLL NSV
Sbjct: 175 NTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 234

Query: 181 LPLGVCVLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSG 240
            PLGVCVL  P+AR+V++VEE+G LEVGF VIFVITIATGVFA VTSVGSISRMLSPLSG
Sbjct: 235 FPLGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 294

Query: 241 VIGILVFLLLPLVVAVVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREGDG 300
           ++GILVFLLLP+VV VVE VK+SV+EGKE RVYH T++E+    R+R ENE KL R+G  
Sbjct: 295 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 354

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAES GS SVSSLVSLSSSF
Sbjct: 355 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSF 414

Query: 361 GFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLS-PSDVSLCVSTAIIAI 420
           GFFGRLMPS+LDH LSR+K MKSK GW+V LMGA+CGGFF LLS P+D+ LCVSTA+IA+
Sbjct: 415 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAV 474

Query: 421 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 480
           CTGAITSISVS TSELFGANNFSINHN++VANIPFGSFLFGYMAA+LY KQA        
Sbjct: 475 CTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATG------ 534

Query: 481 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYSPTNS 527
              TGKCIGVECY+TTFMIWG LCS GTLLAL+LFARTRSFYS T S
Sbjct: 535 --DTGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKS 568

BLAST of CcUC03G042510 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-116
Identity = 251/523 (47.99%), Postives = 338/523 (64.63%), Query Frame = 0

Query: 6   VFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 65
           +  WLSLV I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+    SG+
Sbjct: 5   ILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGI 64

Query: 66  AANYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCY 125
           AA YLPL LVL+ G SLG  GYG+QYL I  +                   ICWINT CY
Sbjct: 65  AAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINTACY 124

Query: 126 MVAITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGV 185
           +VAI +F   NRQVAVGI+ SYQGLS K+YT++V S F     + A  +LLLNS++PL  
Sbjct: 125 IVAINSF-PVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVA 184

Query: 186 CVLASPLAR---VVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGVI 245
           C++ +P+       K +     ++VGFIV+FV+TIATG++A  TS+ S+  +L     ++
Sbjct: 185 CLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV----LV 244

Query: 246 GILVFLLLPLVVAVVEKVKESVKEGKEGRVYH---ITVDENKDVERMRGENEMKLEREGD 305
           GI +FLL PL + +    KE +   K  +  H     VD+   VE    + E + E+   
Sbjct: 245 GIALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKEEEEFEKAII 304

Query: 306 GEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 365
           G   EE+   ++ K+++FW+YFG+YLFG T+GL F NNLGQIAESRGS++ SSLV+LSSS
Sbjct: 305 G-VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLVALSSS 364

Query: 366 FGFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLLSPSDVSLCVSTAIIAI 425
           FGFFGRL+PS+LD+  SR K M S    M G + A+   F LLL  SD++L + TA+I I
Sbjct: 365 FGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMIGI 424

Query: 426 CTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAA 485
            +GA+TS+SV++T+ELFG  +F +NHNI+V +IP GSF FG +AA +Y         AA 
Sbjct: 425 FSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRD------GAAL 484

Query: 486 ADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFYS 523
               GKC G+ C++TT + WG LCS   LLA +L+ R R FYS
Sbjct: 485 YGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of CcUC03G042510 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.3e-73
Identity = 195/534 (36.52%), Postives = 299/534 (55.99%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 68  NYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMV 127
            Y PL +VL   +++G VGYGVQ+L ITN    P Y ++F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 128 AITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVC- 187
            I +F  +NR +A+ ++ S+ G+SA +Y+    +I    +  ++ ++LLLNS++PL V  
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVSF 222

Query: 188 -----VLASPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 247
                VL  P        + +      F ++ V+ + T     ++S  + S  L+     
Sbjct: 223 AALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----F 282

Query: 248 IGILVFLLLPLVVAVVEKVKESVKEGKEGRVYH-------ITVDENKD----VERMRGEN 307
           IG +V L+ PL   ++   ++        R+ H       + +DE K+    V    G  
Sbjct: 283 IGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYE 342

Query: 308 EMKLEREGDGEAM-EEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSS 367
            M   +EG+   + +E   R ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S
Sbjct: 343 HMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 368 VSSLVSLSSSFGFFGRLMPSILDHLLSRKKCMKSKAGWMVGLMGALCGGFFLLL--SPSD 427
            ++LV++ SSF FFGRL+ +  D +   K+   ++ GW    +      FFLL   S   
Sbjct: 403 -TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQ 462

Query: 428 VSLCVSTAIIAICTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLY 487
            +L  +TA+I + +G I + +VSITS+LFG N+  +NHNIL+ NIP GS L+GY+AA +Y
Sbjct: 463 TALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIY 522

Query: 488 HKQAAAAAAAAAADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFY 522
              A+       +D+   CIG +CY  TF+ WG L   G + +L L+ RT+  Y
Sbjct: 523 EANASPDITPIVSDSI-VCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CcUC03G042510 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.1e-73
Identity = 212/562 (37.72%), Postives = 309/562 (54.98%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 68  NYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMV 127
            Y PLW VL   + +G VGYGVQ+L ITN    P Y ++F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 128 AITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVCV 187
            I NF  +NR +A+ ++ S+ G+SA +YT    +I    +  + E++LLLN+++PL V  
Sbjct: 127 CIRNF-PANRSLALSLTVSFNGVSAALYTLAYNAI----NPVSTELYLLLNALVPLFVSF 186

Query: 188 LA-SPLAR--VVKVVEEQG--KLEVGFIVIFVITIATGV----FAAVTSVGSISRMLSPL 247
            A  P+ R   ++ +   G  +  + F+++ ++ +  GV    F + TS  + +R+L   
Sbjct: 187 AALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFGG 246

Query: 248 SGVIGILVFLLLPLVVA---VVEKVKESVKEGKEGRVYHITVDE---------------- 307
           S ++ IL   L  LV A    +  +  S +    G +  + VDE                
Sbjct: 247 SILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFIL-VDVDELEMHKGMVTREASLEG 306

Query: 308 ----NKDVERMRGENEMKLEREGD--------------GEAMEEIGAREMLKRINFWLYF 367
               N DV R     + K   E D              G   EE     +L R +FWLY+
Sbjct: 307 YQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYY 366

Query: 368 GVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDHLLSRKKC 427
             Y  G T+GL + NNLGQIA+S G SS  ++LV+L SSF FFGRL+ +  D++  R K 
Sbjct: 367 IAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI--RAKV 426

Query: 428 MKSKAGWM-VGLMGALCGGFFLLLSPSDVSLCVSTAIIAICTGAITSISVSITSELFGAN 487
             ++ GW+ V L+      F L  S S  +L   TA+I + +G I + +VSITSELFG N
Sbjct: 427 YFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGPN 486

Query: 488 NFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAADATGKCIGVECYKTTFMIW 522
           +  +NHNIL+ NIP GS ++G++AA +Y   + A +   +      C+G +CY  TFM W
Sbjct: 487 SVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESV----ICMGRDCYLQTFMWW 546

BLAST of CcUC03G042510 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 8.4e-70
Identity = 206/564 (36.52%), Postives = 315/564 (55.85%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 68  NYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMV 127
            Y PLW VL   + +G VGYGVQ+L IT+ F S  Y ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITH-FISLPYIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 128 AITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVCV 187
            I+NF  +NR +A+ ++ S+ G+SA +YT    +I    +  + E++LLLN+++PL V  
Sbjct: 127 CISNF-PANRSLALSLTVSFNGVSAALYTLAYNAI----NPTSPELYLLLNALIPLIVSF 186

Query: 188 LA-SPLAR--VVKVVEEQG--KLEVGFIVIFVITIATGV----FAAVTSVGSISRMLSPL 247
            A  P+ R    + +   G  +  + F+++ ++    GV    F + +S  + +R+L   
Sbjct: 187 TAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLL--F 246

Query: 248 SGVIGILVF-LLLPLVVAVVEKVKESVKEG--KEGRVYHITVDENKDVERMRGENEMKLE 307
            G I +LVF L +P +V        ++      EG  + I VD + ++E  +G    +  
Sbjct: 247 GGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGF-ILVDPD-ELELHKGMLAHEAN 306

Query: 308 REG-------------DGEAMEEIGARE-----------------------MLKRINFWL 367
           REG                A+EE  + E                       +L R +FWL
Sbjct: 307 REGYQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWL 366

Query: 368 YFGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDHLLSRK 427
           Y+  Y  G T+GL + NNLGQIA+S G SS+ ++LV+L S+F FFGRL+ +  D++  R 
Sbjct: 367 YYITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI--RA 426

Query: 428 KCMKSKAGWM-VGLMGALCGGFFLLLSPSDVSLCVSTAIIAICTGAITSISVSITSELFG 487
           K   ++ GW+ + L+      F L  S +  +L   TA++ + +G I + +VSITSELFG
Sbjct: 427 KVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFG 486

Query: 488 ANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAAAAAAAAADATGKCIGVECYKTTFM 522
            N+  +NHNIL+ NIP GS ++G++AA +Y         +  +++   C+G +CY  TF+
Sbjct: 487 PNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESV-VCMGRDCYYLTFV 546

BLAST of CcUC03G042510 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-69
Identity = 189/529 (35.73%), Postives = 288/529 (54.44%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 68  NYLPLWLVLMIGSSLGLVGYGVQYLFITNQFQSPSYWVIFSLTVLAGNSICWINTVCYMV 127
            Y P+  VL   +++GLVGYGVQ+L I +    P Y ++     LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 128 AITNFTSSNRQVAVGISTSYQGLSAKVYTNIVGSIFTKHSSKTAEIFLLLNSVLPLGVCV 187
            I +F  +N  +A+ +  S+ G+SA +YT +     +  SS +++I+LLLNS++PL V V
Sbjct: 143 CIRHF-KANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSV 202

Query: 188 LA------SPLARVVKVVEEQGKLEVGFIVIFVITIATGVFAAVTSVGSISRMLSPLSGV 247
           LA      +P +          +  + F+V  V+ + T  +  + S G+     SP    
Sbjct: 203 LALWPVLTNPNSSETDTTRTHDETRI-FVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHF 262

Query: 248 IGILVFLLLPLVVA---VVEKVKESVKEGKEGRVYHITVDENKDVERMRGENEMKLEREG 307
           +G +  LL PL V     + +  ES           + ++E K + +++ +     E   
Sbjct: 263 LGAIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPK-ILKIKSQKSNAEEESD 322

Query: 308 DGEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRG--SSSVSSLVSL 367
                +E     +++++ FWLY+  Y  G T+GL + NNLGQIA+S G  SS+  SLV+L
Sbjct: 323 QVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTL 382

Query: 368 SSSFGFFGRLMPSILDHLLSRKKCMKSKAGWM-VGLMGALCGGFFLLLSP---SDVSLCV 427
            S+F F GRL+ S  D    + K + ++ GW  + L+      F L  SP       L V
Sbjct: 383 FSAFSFLGRLLSSAPDFTRKKLKYL-TRTGWFTISLLPTPLAFFILAYSPKTNQTALLEV 442

Query: 428 STAIIAICTGAITSISVSITSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAA 487
           +TA+I + +G + + +VSITSELFG N+  +N NIL+ NIP GS  +GYMA  +Y   A+
Sbjct: 443 ATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNAS 502

Query: 488 AAAAAAAADATGKCIGVECYKTTFMIWGFLCSFGTLLALILFARTRSFY 522
               +  AD+   C+G +CY  TF+ WG L   G + +L LF RTR  Y
Sbjct: 503 LGRKSVVADSV-VCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVY 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895111.11.2e-24887.50protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida][more]
XP_008457307.15.6e-24185.21PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo][more]
XP_011658866.12.9e-23784.18protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN65862.1 hypothetical pr... [more]
KAA0059993.17.3e-22581.09protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ97252.1 protei... [more]
XP_022970645.19.6e-22581.18protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4I9E11.8e-7236.52Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A1S3C5692.7e-24185.21protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 ... [more]
A0A0A0LYD41.4e-23784.18Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G53369... [more]
A0A5D3BBQ03.5e-22581.09Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1I3F04.6e-22581.18protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A6J1HMC61.5e-22079.89protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G30300.11.2e-11647.99Major facilitator superfamily protein [more]
AT1G31470.11.3e-7336.52Major facilitator superfamily protein [more]
AT5G45275.12.1e-7337.72Major facilitator superfamily protein [more]
AT4G19450.18.4e-7036.52Major facilitator superfamily protein [more]
AT3G01630.11.4e-6935.73Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 205..516
e-value: 8.4E-11
score: 43.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 6..192
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 213..519
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 8..255
e-value: 3.0E-66
score: 223.5
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..522
NoneNo IPR availablePANTHERPTHR21576:SF11MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 3..522

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC03G042510.1CcUC03G042510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane