CcUC01G014470 (gene) Watermelon (PI 537277) v1

Overview


Name	CcUC01G014470
Type	gene
Organism	Citrullus colocynthis (Watermelon (PI 537277) v1)
Description	kunitz trypsin inhibitor 2
Location	CicolChr01: 27237753 .. 27238370 (+)
RNA-Seq Expression	CcUC01G014470
Synteny	CcUC01G014470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTAATGAACGGCTTGCCGACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGATTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGACTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAACAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTAATGAACGGCTTGCCGACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGATTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGACTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAACAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTAATGAACGGCTTGCCGACAACATTTGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGATTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGACTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Protein sequence

MNNLGILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

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BLAST of CcUC01G014470 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 189/205 (92.20%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 1   MNNLGILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLT 60
           M N GILLSFLFILLAST VRFSRADASPEAV DIDG KLRAGVNYYILPVIRGRGGGLT
Sbjct: 1   MKNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 61  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           L NLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LSNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 180
           KLDK DES GQWFVTIGGSRGNPGVETVDNWFKI KHG+DYKLVFCPSVCDFCKVMCRDI
Sbjct: 121 KLDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of CcUC01G014470 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 3.9e-102
Identity = 184/206 (89.32%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MNNLGILLSFLFILLAST-VVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGL 60
           M N GIL  FLFILLAST ++RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDES GQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of CcUC01G014470 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 378.6 bits (971), Expect = 3.3e-101
Identity = 181/203 (89.16%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 3   NLGILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLG 62
           N GI   F+FILLAST +RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGLTLG
Sbjct: 4   NFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 63

Query: 63  NLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKL 122
           NLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 64  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 123

Query: 123 DKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGI 182
           DKFDES GQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIGI
Sbjct: 124 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 183

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFKKV
Sbjct: 184 FFKNGKRALALSDTPFPVMFKKV 205

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BLAST of CcUC01G014470 vs. NCBI nr
Match: KAE8652565.1 (hypothetical protein Csa_014019 [Cucumis sativus])

HSP 1 Score: 364.4 bits (934), Expect = 6.5e-97
Identity = 170/187 (90.91%), Postives = 177/187 (94.65%), Query Frame = 0


Query: 19  VVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQF 78
           ++RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGLTLGNLQSE CPLNVVQEQ 
Sbjct: 62  LIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQL 121

Query: 79  EVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESIGQWFVTIGG 138
           EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTVWKLDKFDES GQW VTIGG
Sbjct: 122 EVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGG 181

Query: 139 SRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPF 198
           SRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIGIFFKNG+RALALSDTPF
Sbjct: 182 SRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSDTPF 241

Query: 199 PVMFKKV 206
           PVMFKKV
Sbjct: 242 PVMFKKV 248

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BLAST of CcUC01G014470 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.8 bits (930), Expect = 1.9e-96
Identity = 173/199 (86.93%), Postives = 183/199 (91.96%), Query Frame = 0


Query: 7   LLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 66
           LLSFLFILLAS  VR SRADASP+AV DIDG KLRAGVNYYILPV RGRGGGL LGNLQS
Sbjct: 6   LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQS 65

Query: 67  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVWKLDKFD
Sbjct: 66  DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 125

Query: 127 ESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 186
           ES  QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+GIFFKN
Sbjct: 126 ESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKN 185

Query: 187 GKRALALSDTPFPVMFKKV 206
           GKRALALSDTPFPVMFK+V
Sbjct: 186 GKRALALSDTPFPVMFKEV 203

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BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 9.5e-59
Identity = 108/198 (54.55%), Postives = 146/198 (73.74%), Query Frame = 0


Query: 8   LSFLFILLASTVV-RFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L ++F+LLA  +  R    +A+ E V DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+  QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

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BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 213.4 bits (542), Expect = 2.4e-54
Identity = 112/208 (53.85%), Postives = 138/208 (66.35%), Query Frame = 0


Query: 8   LSFLFI---LLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNL 67
           LSF F+   L A+     S AD++P  VLDIDG+KLR G NYYI+PV+R  GGGLT+   
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 68  QSE---TCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTIC--ATSTV 127
                  CP  VVQ + EV +  P  F P NPK+ V+RVSTDLN+ F A   C   +STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 128 WKLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKH--GQDYKLVFCPSVCDFCKVMC 187
           W+LDK+DES GQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+VC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 188 RDIGIFF-KNGKRALALSDTPFPVMFKK 205
            D+GI+  + G+R LALSD PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-43
Identity = 96/204 (47.06%), Postives = 126/204 (61.76%), Query Frame = 0


Query: 6   ILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGR-GGGLTLGNL 65
           +LL F F    S    F  A+A+   VLD DGD+L+ GV YY+L  I G  GGGL LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 66  QSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE--ASTICATSTVWKL 125
             ++CP  VVQ + ++ NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 126 DKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHG-QDYKLVFCPSVCDFCKVMCRDIG 185
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 186 IFF-KNGKRALALSDTPFPVMFKK 205
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.6e-37
Identity = 88/196 (44.90%), Postives = 118/196 (60.20%), Query Frame = 0


Query: 12  FILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 71
           +++LA T V  S A     AV+DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESIG 131
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGQD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

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BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot
Match: Q41015 (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum OX=3888 GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.7e-26
Identity = 76/198 (38.38%), Postives = 103/198 (52.02%), Query Frame = 0


Query: 10  FLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRG-RGGGLTLGNLQSET 69
           FLF+ + +  + FS  D   E VLD +G+ +  GV Y+ILP IRG  GGG+ LG     T
Sbjct: 12  FLFVFITNLSLAFSNEDV--EQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTGDLT 71

Query: 70  CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDES 129
           CP+ V+Q++ EV NGLP  F       G+I   T + +++     CA S+ W L   D  
Sbjct: 72  CPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKW-LVFVDNV 131

Query: 130 IGQWFVTIGGSRGNPGVETVDNWFKIEKH--GQDYKLVFCPSVCDFCKVMCRDIGIF--F 189
           I +  V IGG    PGV+T+   FKIEKH  G  YKL FC          C D+G F   
Sbjct: 132 IQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFCVK----GSPTCLDVGRFDND 191

Query: 190 KNGKRALALSDTPFPVMF 203
           ++G+R        F V+F
Sbjct: 192 EDGRRLNLTEHESFQVVF 202

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BLAST of CcUC01G014470 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.9e-102
Identity = 184/206 (89.32%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MNNLGILLSFLFILLAST-VVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGL 60
           M N GIL  FLFILLAST ++RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDES GQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of CcUC01G014470 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.6e-101
Identity = 181/203 (89.16%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 3   NLGILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLG 62
           N GI   F+FILLAST +RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGLTLG
Sbjct: 4   NFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 63

Query: 63  NLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKL 122
           NLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 64  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 123

Query: 123 DKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGI 182
           DKFDES GQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIGI
Sbjct: 124 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 183

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFKKV
Sbjct: 184 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of CcUC01G014470 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.6e-101
Identity = 181/203 (89.16%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 3   NLGILLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLG 62
           N GI   F+FILLAST +RFS ADASPEAVLDIDG KLRAGVNYYILPV RGRGGGLTLG
Sbjct: 4   NFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 63

Query: 63  NLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKL 122
           NLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 64  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 123

Query: 123 DKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGI 182
           DKFDES GQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIGI
Sbjct: 124 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 183

Query: 183 FFKNGKRALALSDTPFPVMFKKV 206
           FFKNGKRALALSDTPFPVMFKKV
Sbjct: 184 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of CcUC01G014470 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.2e-97
Identity = 173/199 (86.93%), Postives = 183/199 (91.96%), Query Frame = 0


Query: 7   LLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 66
           LLSFLFILLAS  VR SRADASP+AV DIDG KLRAGVNYYILPV RGRGGGL LGNLQS
Sbjct: 6   LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQS 65

Query: 67  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVWKLDKFD
Sbjct: 66  DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 125

Query: 127 ESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 186
           ES  QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+GIFFKN
Sbjct: 126 ESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKN 185

Query: 187 GKRALALSDTPFPVMFKKV 206
           GKRALALSDTPFPVMFK+V
Sbjct: 186 GKRALALSDTPFPVMFKEV 203

[Close]

BLAST of CcUC01G014470 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.2e-97
Identity = 173/199 (86.93%), Postives = 183/199 (91.96%), Query Frame = 0


Query: 7   LLSFLFILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 66
           LLSFLFILLAS  VR SRADASP+AV DIDG KLRAGVNYYILPV RGRGGGL LGNLQS
Sbjct: 6   LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQS 65

Query: 67  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVWKLDKFD
Sbjct: 66  DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 125

Query: 127 ESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 186
           ES  QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+GIFFKN
Sbjct: 126 ESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKN 185

Query: 187 GKRALALSDTPFPVMFKKV 206
           GKRALALSDTPFPVMFK+V
Sbjct: 186 GKRALALSDTPFPVMFKEV 203

[Close]

BLAST of CcUC01G014470 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 228.0 bits (580), Expect = 6.8e-60
Identity = 108/198 (54.55%), Postives = 146/198 (73.74%), Query Frame = 0


Query: 8   LSFLFILLASTVV-RFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 67
           L ++F+LLA  +  R    +A+ E V DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+  QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of CcUC01G014470 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 156.0 bits (393), Expect = 3.3e-38
Identity = 88/196 (44.90%), Postives = 118/196 (60.20%), Query Frame = 0


Query: 12  FILLASTVVRFSRADASPEAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 71
           +++LA T V  S A     AV+DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESIG 131
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKHGQD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of CcUC01G014470 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-24
Identity = 73/208 (35.10%), Postives = 117/208 (56.25%), Query Frame = 0


Query: 6   ILLSFLFILLASTV-VRFSRADASP-EAVLDIDGDKLRAGVNYYILPVIRGRGGGLTLGN 65
           + LSF+ + + S +    S ADA+P + VLDI G  +++ V YYI+P   G GGGL   N
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 66  LQSET----CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 125
               T      L++VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 126 VWKLDKFDESIGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCR 185
           VW++D     + + FV+IGG +G       ++WF+I++ G  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 186 DIGI-FFKNGKRALALS-DTPFPVMFKK 205
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of CcUC01G014470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895203.1	2.2e-105	92.20	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
XP_004137534.1	3.9e-102	89.32	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]
XP_008437058.1	3.3e-101	89.16	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
KAE8652565.1	6.5e-97	90.91	hypothetical protein Csa_014019 [Cucumis sativus]	[more]
XP_022923703.1	1.9e-96	86.93	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]

BLAST of CcUC01G014470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	9.5e-59	54.55	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	2.4e-54	53.85	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	1.5e-43	47.06	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	4.6e-37	44.90	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
Q41015	3.7e-26	38.38	Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum OX=3888 GN=PIP20-1...	[more]

BLAST of CcUC01G014470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTW2	1.9e-102	89.32	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]
A0A5D3BIG0	1.6e-101	89.16	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	1.6e-101	89.16	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A6J1KRM6	9.2e-97	86.93	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	9.2e-97	86.93	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]

BLAST of CcUC01G014470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	6.8e-60	54.55	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	3.3e-38	44.90	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.2e-24	35.10	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 151..170 score: 25.39 coord: 174..203 score: 25.03 coord: 31..60 score: 47.06 coord: 69..89 score: 34.45
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 e-value: 1.4E-76 score: 270.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 e-value: 8.2E-61 score: 205.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 31..204 e-value: 1.03949E-59 score: 182.927
None	No IPR available	GENE3D	2.80.10.50		coord: 26..205 e-value: 1.6E-77 score: 261.1
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CcUC01G014470.1	CcUC01G014470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity