CcUC01G014340 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC01G014340
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCicolChr01: 27107520 .. 27108675 (+)
RNA-Seq ExpressionCcUC01G014340
SyntenyCcUC01G014340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGGAAGCGAGATCCCAATTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAATGAAGGAGTGGGTTTTCACAAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGATGTTTTGTAGCATTATACGACACGGTTTCAATGGAACTCAGTTCCAACATGTTGGGATCCTTAAAGCAATTGTTTGATGTTCCATTGGAGAGGAAAATTCAAAATGTGTCTGAAAAGCCATACCATGGCTATTTTGGTCAAAATCCTCTCATGCCAATCCATGAAAGTATGGGCATCGAACACCCTATTCTCCCCACCAACATTCATTCCTTCACTAATCTCATGTGGCCGTCTCGTGGAAACAAGCCTTTCTGGTATATATTTTTCAATTATTTTTGAAATATTATCCTATTACAAACTACTTGTACTAACTATTTCTGTGATCTGCAGTGAAAATGTAATGACGTACGCGAAGTTAGTATCGAAGTTAGACGAGAGGGTAAAAAACATGGTGTTTGAAAGCTATGGGGTTGAAAATCAGTTAAAAAGTCATATGGAATCCACCAAATATCTTCTAAGAATGATAAAATATAGAGTCCCAAAGGAAAAGGAGATGAATTTGGGAGCATTTCCTCACACTGACAAGAGCTTCCTCACAATTCTTCACCAAAATGAAGTGAATGGCTTACAAATCAAAACTAGAGATAACAAGTGGCTTCAATACCATCCTTCTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGGGATGCCTTCTTTGTAAGTTCGTTTTAAATAATATCTCTTATCTCAAAATTATATTTTATTAAATTAATTAATTAACCCAAAGTGATTAATTAACAGGCCTGGAGCAACGGGAGGATTTACTCTCCACCTCACAGGGTGATAATGAATGGAAACAGAGAGAGATATTCGGTGGGTTTGTTTTCTTTCAATGATGGAATTATTCAAATTCCTAAAGAATTGGTAGACGACAAACATCCGCAACAATTTAAGCCCTTTGATCATCAAGATTATCTTCGTTTCTACAGCACTGAGAGAGGCCAAAATTCCCCTTGTGCTATCAAAGCTTATTGTGGGATCGGAAATGCGGGCTTTGTTGATACTGATACTCGAAGCGCTCTTCTCTTGCATTAA

mRNA sequence

ATGGGAGGAAGCGAGATCCCAATTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAATGAAGGAGTGGGTTTTCACAAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGATGTTTTGTAGCATTATACGACACGGTTTCAATGGAACTCAGTTCCAACATGTTGGGATCCTTAAAGCAATTGTTTGATGTTCCATTGGAGAGGAAAATTCAAAATGTGTCTGAAAAGCCATACCATGGCTATTTTGGTCAAAATCCTCTCATGCCAATCCATGAAAGTATGGGCATCGAACACCCTATTCTCCCCACCAACATTCATTCCTTCACTAATCTCATGTGGCCGTCTCGTGGAAACAAGCCTTTCTGTGAAAATGTAATGACGTACGCGAAGTTAGTATCGAAGTTAGACGAGAGGGTAAAAAACATGGTGTTTGAAAGCTATGGGGTTGAAAATCAGTTAAAAAGTCATATGGAATCCACCAAATATCTTCTAAGAATGATAAAATATAGAGTCCCAAAGGAAAAGGAGATGAATTTGGGAGCATTTCCTCACACTGACAAGAGCTTCCTCACAATTCTTCACCAAAATGAAGTGAATGGCTTACAAATCAAAACTAGAGATAACAAGTGGCTTCAATACCATCCTTCTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGGGATGCCTTCTTTGCCTGGAGCAACGGGAGGATTTACTCTCCACCTCACAGGGTGATAATGAATGGAAACAGAGAGAGATATTCGGTGGGTTTGTTTTCTTTCAATGATGGAATTATTCAAATTCCTAAAGAATTGGTAGACGACAAACATCCGCAACAATTTAAGCCCTTTGATCATCAAGATTATCTTCGTTTCTACAGCACTGAGAGAGGCCAAAATTCCCCTTGTGCTATCAAAGCTTATTGTGGGATCGGAAATGCGGGCTTTGTTGATACTGATACTCGAAGCGCTCTTCTCTTGCATTAA

Coding sequence (CDS)

ATGGGAGGAAGCGAGATCCCAATTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAATGAAGGAGTGGGTTTTCACAAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGATGTTTTGTAGCATTATACGACACGGTTTCAATGGAACTCAGTTCCAACATGTTGGGATCCTTAAAGCAATTGTTTGATGTTCCATTGGAGAGGAAAATTCAAAATGTGTCTGAAAAGCCATACCATGGCTATTTTGGTCAAAATCCTCTCATGCCAATCCATGAAAGTATGGGCATCGAACACCCTATTCTCCCCACCAACATTCATTCCTTCACTAATCTCATGTGGCCGTCTCGTGGAAACAAGCCTTTCTGTGAAAATGTAATGACGTACGCGAAGTTAGTATCGAAGTTAGACGAGAGGGTAAAAAACATGGTGTTTGAAAGCTATGGGGTTGAAAATCAGTTAAAAAGTCATATGGAATCCACCAAATATCTTCTAAGAATGATAAAATATAGAGTCCCAAAGGAAAAGGAGATGAATTTGGGAGCATTTCCTCACACTGACAAGAGCTTCCTCACAATTCTTCACCAAAATGAAGTGAATGGCTTACAAATCAAAACTAGAGATAACAAGTGGCTTCAATACCATCCTTCTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGGGATGCCTTCTTTGCCTGGAGCAACGGGAGGATTTACTCTCCACCTCACAGGGTGATAATGAATGGAAACAGAGAGAGATATTCGGTGGGTTTGTTTTCTTTCAATGATGGAATTATTCAAATTCCTAAAGAATTGGTAGACGACAAACATCCGCAACAATTTAAGCCCTTTGATCATCAAGATTATCTTCGTTTCTACAGCACTGAGAGAGGCCAAAATTCCCCTTGTGCTATCAAAGCTTATTGTGGGATCGGAAATGCGGGCTTTGTTGATACTGATACTCGAAGCGCTCTTCTCTTGCATTAA

Protein sequence

MGGSEIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQNSPCAIKAYCGIGNAGFVDTDTRSALLLH
Homology
BLAST of CcUC01G014340 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 626.3 bits (1614), Expect = 1.5e-175
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 3   GSEIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQL 62
           G EIPIVKFCSENLKSG KEWV TREKVREALEEYGCFVALYDTVSM++SSNM  SLKQL
Sbjct: 2   GGEIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQL 61

Query: 63  FDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPF 122
           FDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPS GN+PF
Sbjct: 62  FDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPF 121

Query: 123 CENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGA 182
           CENV  YAKLVS+LDERVK+MVFESYGV   LK+HMESTKYL+RMIKYRVPKEKEMNLGA
Sbjct: 122 CENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGA 181

Query: 183 FPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIY 242
           FPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHP SSSSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 182 FPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIY 241

Query: 243 SPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQ 302
           SPPHRV+++ N+ERYSVGLFSFNDGIIQIPKELVDDKHPQQF PFDHQDYLRFY+TERGQ
Sbjct: 242 SPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQ 301

Query: 303 NSPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           NSPCAIKAYCG+GN+  VDTD+RS LLLH
Sbjct: 302 NSPCAIKAYCGVGNS-LVDTDSRSTLLLH 329

BLAST of CcUC01G014340 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 295/327 (90.21%), Postives = 311/327 (95.11%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           +IPIVKFCSENLKSG KEWV TREKVREALEEYGCFVALYDTVSM++SSNM  SLKQLFD
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 64

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPS GN+PFCE
Sbjct: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFCE 124

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAFP 184
           NV  YAKLVS+LDERVK+MVFESYGV   LK+HMESTKYL+RMIKYRVPKEKEMNLGAFP
Sbjct: 125 NVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAFP 184

Query: 185 HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYSP 244
           HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHP SSSSTSSFIVMAGDAFFAWSNGRIYSP
Sbjct: 185 HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYSP 244

Query: 245 PHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQNS 304
           PHRV+++ N+ERYSVGLFSFNDGIIQIPKELVDDKHPQQF PFDHQDYLRFY+TERGQNS
Sbjct: 245 PHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQNS 304

Query: 305 PCAIKAYCGIGNAGFVDTDTRSALLLH 332
           PCAIKAYCG+GN+  VDTD+RS LLLH
Sbjct: 305 PCAIKAYCGVGNS-LVDTDSRSTLLLH 330

BLAST of CcUC01G014340 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 601.7 bits (1550), Expect = 3.9e-168
Identity = 287/328 (87.50%), Postives = 308/328 (93.90%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLF- 64
           E+P+VKFCSENLKSG KEWVFT +KVREALE+YGCFVALYD+VS E+SSNM  SLK+LF 
Sbjct: 8   ELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFD 67

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPSRGN  F 
Sbjct: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
           E+V TYAKLVS+LDERVK MVFESYGV N LKSHMESTKYL+RMIKYRVPKEKEMNLGAF
Sbjct: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYS 244
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 247

Query: 245 PPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 304
           PPHRVIM+GNRERYSVGLFSFN+GI+QIP+ELVDDKHPQ F PFDHQD+LRFYSTERGQN
Sbjct: 248 PPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYSTERGQN 307

Query: 305 SPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           SPCAIKAYCG+GN+  +D DTRS LLLH
Sbjct: 308 SPCAIKAYCGVGNS-LIDMDTRSPLLLH 334

BLAST of CcUC01G014340 vs. NCBI nr
Match: XP_031738134.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571.1 hypothetical protein Csa_014276 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 5.1e-168
Identity = 287/332 (86.45%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MGGSEIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLK 60
           M   ++P+VKFCS+NLKSG +EWV T +KVREALEEYGCFVALYDTVS E+SSNM  SLK
Sbjct: 8   MSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLK 67

Query: 61  QLFD-VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGN 120
           +LFD VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPSRGN
Sbjct: 68  ELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 127

Query: 121 KPFCENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMN 180
             F ENV  YA LVS+LD+RVK+MVFESYGV N L+SHMESTKYL+RMIKYRVPKEKEMN
Sbjct: 128 HSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMN 187

Query: 181 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNG 240
           LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNG
Sbjct: 188 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 247

Query: 241 RIYSPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYSPPHRVIM+GN+ERYSVGLFSFN+GI+QIPKELVDDKHPQ FKPFDHQDYLRFYSTE
Sbjct: 248 RIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTE 307

Query: 301 RGQNSPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           RGQNSPCAIKAYCG+GN+  VDTDTRS LLLH
Sbjct: 308 RGQNSPCAIKAYCGVGNS-LVDTDTRSPLLLH 338

BLAST of CcUC01G014340 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 600.9 bits (1548), Expect = 6.7e-168
Identity = 286/332 (86.14%), Postives = 309/332 (93.07%), Query Frame = 0

Query: 1   MGGSEIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLK 60
           M   ++P+VKFCS+NLKSG +EWV T +KVREALEEYGCFVALYDTVS E+SSNM  SLK
Sbjct: 8   MSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLK 67

Query: 61  QLFD-VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGN 120
           +LFD VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPSRGN
Sbjct: 68  ELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 127

Query: 121 KPFCENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMN 180
             F ENV  YA LVS+LD+RVK+MVFESYGV N L+SHMESTKYL+RMIKYRVPKEKEMN
Sbjct: 128 HSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMN 187

Query: 181 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNG 240
           LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNG
Sbjct: 188 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 247

Query: 241 RIYSPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYSPPHRVIM+GN+ERYSVGLFSFN+GI+QIPKELVDDKHPQ FKPFDHQDYLRFYSTE
Sbjct: 248 RIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTE 307

Query: 301 RGQNSPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           RGQNSPCAIKAYCG+GN+  +DTDTRS LLLH
Sbjct: 308 RGQNSPCAIKAYCGVGNS-LIDTDTRSPLLLH 338

BLAST of CcUC01G014340 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-72
Identity = 130/314 (41.40%), Postives = 203/314 (64.65%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           ++P++ F  +NLK G  +W   +  V +ALE+YGCF A +D +S+EL+ ++  +++ LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           +P+  K +NVS KP+HGY   N    ++ES+GI+   +   ++ FT  +WP  GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVP-----KEKEMN 184
            +  +++ + +LD  V+ M+ ES+G+EN +  H+ ST YL R++KY  P      ++E  
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEETK 192

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNG 244
           LG   HTDK+ +TILHQ +V+GL++KT+D+KW++  PS      S +VM GD+  A  NG
Sbjct: 193 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQ----DSVLVMVGDSLCALLNG 252

Query: 245 RIYSPPHRVIMNGNRERYSVGLFSF--NDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYS 304
           R++SP HRVIM G + RYS GLFS      II  P+ELVD +HP+ FKPF++ D+L F+ 
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 312

Query: 305 TERGQNSPCAIKAY 312
           TE G+ +  A+ A+
Sbjct: 313 TEAGRIAQSALHAF 318

BLAST of CcUC01G014340 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 130/315 (41.27%), Postives = 201/315 (63.81%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           ++P++ F  +NLK G  +W      V +ALE+YGCF A +D +S+EL+ ++  +++ LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           +P+  K +NVS KP+HGY   N    ++ES+GI    +   ++ FT  +WP  GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVP------KEKEM 184
            +  +++ + +LD  V+ M+ ES+G+EN +  H+ ST YL R++KY  P       ++E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 185 NLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSN 244
            LG   HTDK+ +TILHQ +V+GL++KT+D+KW++  PS      S +VM GD+  A  N
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQ----DSVLVMVGDSLCALLN 252

Query: 245 GRIYSPPHRVIMNGNRERYSVGLFSF--NDGIIQIPKELVDDKHPQQFKPFDHQDYLRFY 304
           GR++SP HRVIM G + RYS GLFS      II  P+ELVD +HP+ FKPF++ D+L F+
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 312

Query: 305 STERGQNSPCAIKAY 312
            TE G+ +  A+ A+
Sbjct: 313 QTEAGRIAQSALHAF 319

BLAST of CcUC01G014340 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.4e-69
Identity = 126/304 (41.45%), Postives = 194/304 (63.82%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           ++P++ F  +NLK G  +W      V +ALE+YGCF A +D +S+EL+ ++  +++ LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           +P+  K +NVS K +HGY   N    ++ES+GI    +   ++ FT  +WP  GNK   E
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVP----KEKEMNL 184
            +  +++ + +LD  V+ M+ ES+G+E  +  H+ ST YL R++KY  P     ++E  L
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKL 185

Query: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGR 244
           G   HTDK+ +TILHQ +V+GL++KT+D+KW++  PS      S +VM GD+  A  NGR
Sbjct: 186 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQ----DSVLVMVGDSLCALLNGR 245

Query: 245 IYSPPHRVIMNGNRERYSVGLFSF--NDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYST 303
           ++SP HRVIM G + RYS GLFS      II  P+ELVD +HP+ FKPF++ D+L F+ T
Sbjct: 246 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 301

BLAST of CcUC01G014340 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.5e-63
Identity = 124/306 (40.52%), Postives = 180/306 (58.82%), Query Frame = 0

Query: 6   IPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFDV 65
           +P++    +NLK G   W   R +VREALEEYGCF A+ D VS EL   +     +L ++
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLNL 68

Query: 66  PLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCEN 125
           PLE K+ N ++  Y G+     +  ++E MG+        +  FTNLMWP  GNK FCE 
Sbjct: 69  PLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCET 128

Query: 126 VMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAFPH 185
           V +Y K ++++D  +K MVFES+G+E    S  EST YLL  ++Y+ P +   +     H
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSLH 188

Query: 186 TDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYSPP 245
            DKS LTI++QN+V GL+ +T+D +W+        +  + IV+ GD F AWSNGR++SP 
Sbjct: 189 KDKSILTIVNQNDVKGLEFETKDGEWI------LPTADNHIVLLGDCFMAWSNGRLHSPL 248

Query: 246 HRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQNSP 305
           HRV +  N+ R S   FSF   II+ P ELVD++HP  F PF+  + L +  T+ G  + 
Sbjct: 249 HRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAV 304

Query: 306 CAIKAY 312
           C +K Y
Sbjct: 309 CDLKQY 304

BLAST of CcUC01G014340 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.7e-55
Identity = 133/409 (32.52%), Postives = 197/409 (48.17%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           ++P++    + LK G  +WV  R  VR+ALE+YGCF A  D VSMEL  ++L ++++LF 
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLKAMQELFA 66

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           +P E K +NV  KP+ GY   N L    ES GI+   +    H FT  +WP  GNK   +
Sbjct: 67  LPTEAKQRNVCPKPFTGYLSHNGL---SESFGIKDANILEKAHEFTQQLWP-EGNKSISK 126

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKE--------- 184
            +  YA+ +++LD  V+ ++ ESYG+E  +  H+ ST Y +R++KY    +         
Sbjct: 127 MIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGA 186

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 187 NVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDLD 246

Query: 245 -----------------------KEMN----LGAFPHTDKSFLTILHQNEVNGLQIKTRD 304
                                  KE N    LG   HTDKS   I++Q++++GL++KT++
Sbjct: 247 AEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTKE 306

Query: 305 NKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRV-IMNGNRERYSVGLFSF-ND 315
            KW++  P    + ++ IV+AGDA  A  NGRI SP HRV +    + RY+  LFS   +
Sbjct: 307 GKWIRVKP----APNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSNPKE 366

BLAST of CcUC01G014340 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 287/328 (87.50%), Postives = 308/328 (93.90%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLF- 64
           E+P+VKFCSENLKSG KEWVFT +KVREALE+YGCFVALYD+VS E+SSNM  SLK+LF 
Sbjct: 8   ELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKELFD 67

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPSRGN  F 
Sbjct: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
           E+V TYAKLVS+LDERVK MVFESYGV N LKSHMESTKYL+RMIKYRVPKEKEMNLGAF
Sbjct: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYS 244
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 247

Query: 245 PPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 304
           PPHRVIM+GNRERYSVGLFSFN+GI+QIP+ELVDDKHPQ F PFDHQD+LRFYSTERGQN
Sbjct: 248 PPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYSTERGQN 307

Query: 305 SPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           SPCAIKAYCG+GN+  +D DTRS LLLH
Sbjct: 308 SPCAIKAYCGVGNS-LIDMDTRSPLLLH 334

BLAST of CcUC01G014340 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 267/332 (80.42%), Postives = 294/332 (88.55%), Query Frame = 0

Query: 1   MGGSEIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLK 60
           M   ++P+VKF  ENL+S    WV TREKVREALEEYGCFVALYD+VSMELSSNM  SLK
Sbjct: 1   MAEFQLPLVKFSEENLRS----WVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLK 60

Query: 61  QLFDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNK 120
           +LF+VPLERK++NVSEKPYHGYFGQNPLMPIHESMGIE P L +   SFTNLMWPS GNK
Sbjct: 61  ELFEVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNK 120

Query: 121 PFCENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNL 180
            FCENV +YA+ +++LDE VK MVFESYGV   L+SHM+ST+YLLRMIKYRVPK+ EMN+
Sbjct: 121 SFCENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNM 180

Query: 181 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHP-SSSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN+VNGLQIKT+ NKWLQYHP SSSSSTSSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNG 240

Query: 241 RIYSPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYSPPH+VIM+G RERYSVGLFSFNDGI+QIPKELVD+ HPQQFKPFDHQDYLRFYSTE
Sbjct: 241 RIYSPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTE 300

Query: 301 RGQNSPCAIKAYCGIGNAGFVDTDTRSALLLH 332
           RGQNSPCAIKAYCGIGN G V  DTR  +LLH
Sbjct: 301 RGQNSPCAIKAYCGIGN-GLV--DTRGCILLH 325

BLAST of CcUC01G014340 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.8e-142
Identity = 245/322 (76.09%), Postives = 283/322 (87.89%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSS-NMLGSLKQLF 64
           EIP+V+F S NL+SG ++W  TREKVREALEEYGCFVALYD+VSME SS  M+ ++K++F
Sbjct: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  FC
Sbjct: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
           EN+ +YA+LVS+L++ VK MVFESYG+++   SH +STKYLLRMIKYRVP   EMNLGAF
Sbjct: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYH-PSSSSSTSSFIVMAGDAFFAWSNGRIY 244
           PHTDKSFLTILHQN VNGL IKTRDN+W++Y  PS+ SS  SFIVMAGDAFFAWSNGRIY
Sbjct: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRIY 243

Query: 245 SPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQ 304
           S PHRV M+GNRERYSVGLFSFNDGI+QIPKELVDDKHPQQFK FDHQDYLRFYSTE+GQ
Sbjct: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 303

Query: 305 NSPCAIKAYCGIGNAGFVDTDT 325
           +SP AIKAYCG+ N G +D+ T
Sbjct: 304 SSPSAIKAYCGVAN-GLLDSRT 324

BLAST of CcUC01G014340 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.3e-140
Identity = 241/322 (74.84%), Postives = 282/322 (87.58%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSS-NMLGSLKQLF 64
           E+P+V+F S NL+SG ++W  TREKVREALEEYGCFVAL D+VSME SS  M+ +++++F
Sbjct: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  FC
Sbjct: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
           EN+ +YA+LVS+L++ VK MVFESYG+++   SH +STKYLLRMIKYRVP   EMNLGAF
Sbjct: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYH-PSSSSSTSSFIVMAGDAFFAWSNGRIY 244
           PHTDKSFLTILHQN VNGL IKTRDN+W++Y  PS+ SS  SFI+MAGDAFFAWSNGRIY
Sbjct: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIY 243

Query: 245 SPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQ 304
           S PHRV M+GNRERYSVGLFSFNDGI+QIPKELVDDKHPQQFK FDHQDYLRFYSTE+GQ
Sbjct: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 303

Query: 305 NSPCAIKAYCGIGNAGFVDTDT 325
           +SP AIKAYCG+ N G +D+ T
Sbjct: 304 SSPSAIKAYCGVAN-GLLDSRT 324

BLAST of CcUC01G014340 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.3e-140
Identity = 241/322 (74.84%), Postives = 281/322 (87.27%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSS-NMLGSLKQLF 64
           EIP+V+F S N++SG ++W  TREKVREALEEYGCFVAL D+VSME SS  M+ ++K++F
Sbjct: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           +VPLERK+QNVS+KP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  FC
Sbjct: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
            N+ +YA+LVS+L++ VK MVFESYG+++   SH +STKYLLRMIKYRVP   EMNLGAF
Sbjct: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYH-PSSSSSTSSFIVMAGDAFFAWSNGRIY 244
           PHTDKSFLTILHQN VNGL IKTRDN+W++Y  PS+ SS  SFIVMAGDAFFAWSNGRIY
Sbjct: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIY 243

Query: 245 SPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQ 304
           S PHRV M+GNRERYSVGLFSFNDGI+QIPKELVDDKHPQQFK FDHQDYLRFYSTE+GQ
Sbjct: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 303

Query: 305 NSPCAIKAYCGIGNAGFVDTDT 325
           +SP AIKAYCG+ N G +D+ T
Sbjct: 304 SSPSAIKAYCGVAN-GLLDSRT 324

BLAST of CcUC01G014340 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.0e-86
Identity = 151/310 (48.71%), Postives = 213/310 (68.71%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVA-LYDTVSMELSSNMLGSLKQLF 64
           +IP + F  E+LK G K W  TRE +R+ALEEYGCF+  L D   ++L   + GSL  LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFC 124
           D+P + K++N  +KP +GY GQ P +P+HES+GI++        SFT LMWP +GN+ F 
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFS 123

Query: 125 ENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAF 184
           E +  YA+  ++LD+ V  MVF+SY VE     ++EST YLLR++K R P  +   LG  
Sbjct: 124 ECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGFV 183

Query: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYS 244
            HTDKSF TILHQ++VNGL+++TR+ + +     + SS S F+V+AGDA  AWSN R++S
Sbjct: 184 THTDKSFTTILHQDQVNGLEMETREGERINI---NLSSPSLFMVVAGDALMAWSNDRVWS 243

Query: 245 PPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 304
           P H+V+++G  +RYS+G+F+FN+G +Q+P+EL+D +HP  +KPFDH   LRFY T+ G  
Sbjct: 244 PRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYK 303

Query: 305 SPCAIKAYCG 314
           S C IKAYCG
Sbjct: 304 SECPIKAYCG 309

BLAST of CcUC01G014340 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 7.5e-85
Identity = 140/311 (45.02%), Postives = 215/311 (69.13%), Query Frame = 0

Query: 6   IPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFDV 65
           +P++ F ++NLK G  EW  TR  V++AL++YG F A +D +  EL  ++ G+L++LFD+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 66  PLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCEN 125
           PL+ K++NVS+KP+HGY GQ P++P++ESMGI+   +   + +FT  +WP +GN  F   
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFSTT 131

Query: 126 VMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGAFPH 185
           + +++K +S+LD  ++ M+ ES+G++  +  H+ ST YLLR++KY+ P  +E  +G   H
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNAH 191

Query: 186 TDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIYSPP 245
           TDK+ +TIL+QN V GL+++T+D  W++  P+      SF VM GD+ +A  NGR++SP 
Sbjct: 192 TDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQ----DSFTVMIGDSLYALLNGRLHSPY 251

Query: 246 HRVIMNGNRERYSVGLFSFNDG--IIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 305
           HRV+M G   RYS+GLFS      I+  P ELVD++HP+ FKPFDH ++L+FY TE GQ 
Sbjct: 252 HRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQR 311

Query: 306 SPCAIKAYCGI 315
           S  A+K YCGI
Sbjct: 312 SQSALKTYCGI 317

BLAST of CcUC01G014340 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 9.2e-83
Identity = 145/313 (46.33%), Postives = 211/313 (67.41%), Query Frame = 0

Query: 4   SEIPIVKFCS-ENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQL 63
           +++PI+   S + LK     W     +  EALEEYGCF+A+YD V+ +L  ++  + ++L
Sbjct: 8   TKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEEL 67

Query: 64  FDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPF 123
           FD+P E K +NV+EKPYHGY GQ P++P+HE +G+++         FT+LMWP +GN  F
Sbjct: 68  FDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 127

Query: 124 CENVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVPKEKEMNLGA 183
           C  V T++  V++LD  V  M+FE+YGVE   +SH+ S  YLL+ +KY  P E  +++ A
Sbjct: 128 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPE-SISMPA 187

Query: 184 FP-HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRI 243
           FP HTDK+FL+ILHQN+VNGL++K++D +W+    S      S++VMAGD    WSN RI
Sbjct: 188 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWI----SLQLPPKSYVVMAGDISMGWSNDRI 247

Query: 244 YSPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYSTERG 303
            S  HRV M G++ RY++GLFSF   ++ IP+ELVDDKHP  +KPFD+   + FY+T+ G
Sbjct: 248 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 307

Query: 304 QNSPCAIKAYCGI 315
           + +   +KAYCGI
Sbjct: 308 REANSTLKAYCGI 314

BLAST of CcUC01G014340 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 130/315 (41.27%), Postives = 201/315 (63.81%), Query Frame = 0

Query: 5   EIPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFD 64
           ++P++ F  +NLK G  +W      V +ALE+YGCF A +D +S+EL+ ++  +++ LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 124
           +P+  K +NVS KP+HGY   N    ++ES+GI    +   ++ FT  +WP  GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 125 NVMTYAKLVSKLDERVKNMVFESYGVENQLKSHMESTKYLLRMIKYRVP------KEKEM 184
            +  +++ + +LD  V+ M+ ES+G+EN +  H+ ST YL R++KY  P       ++E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 185 NLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSN 244
            LG   HTDK+ +TILHQ +V+GL++KT+D+KW++  PS      S +VM GD+  A  N
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQ----DSVLVMVGDSLCALLN 252

Query: 245 GRIYSPPHRVIMNGNRERYSVGLFSF--NDGIIQIPKELVDDKHPQQFKPFDHQDYLRFY 304
           GR++SP HRVIM G + RYS GLFS      II  P+ELVD +HP+ FKPF++ D+L F+
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 312

Query: 305 STERGQNSPCAIKAY 312
            TE G+ +  A+ A+
Sbjct: 313 QTEAGRIAQSALHAF 319

BLAST of CcUC01G014340 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 131/315 (41.59%), Postives = 197/315 (62.54%), Query Frame = 0

Query: 6   IPIVKFCSENLKSGMKEWVFTREKVREALEEYGCFVALYDTVSMELSSNMLGSLKQLFDV 65
           +PI+ F S+ L  G   W+ TR+ VR A+E  G FVA +  VS +L  N+L  +K+++ +
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 66  PLERKIQNVSEKPYHGYFGQ-NPLMPIHESMGIEHPILPTNIHSFTNLMWPSRGNKPFCE 125
           P + KI+N + K  HGY         IHES+GI++         F+ L+WP +GN PFC+
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 126 NVMTYAKLVSKLDERVKNMVFESYGVENQLK--SHMESTKYLLRMIKYRVPKEKEMNLGA 185
               YA  +++LD+ V  M++ESYG++ +    SH EST+YLLRM+ YR  +  E N G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 186 FPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPSSSSSTSSFIVMAGDAFFAWSNGRIY 245
             HTDKSF++ILHQN V GLQ+KT   +W+ ++P    S + F+V++G    AWSN RI 
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP----SPTRFVVLSGMGLTAWSNDRIK 248

Query: 246 SPPHRVIMNGNRERYSVGLFSFNDGIIQIPKELVDDKHPQQFKPFDHQDYLRFYST---E 305
           +  H+V+M+ +  RYS+G FSF+ G I+ P+ELVDD+HP ++ PF+H   LRFY +    
Sbjct: 249 ACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINS 308

Query: 306 RGQNSPCAIKAYCGI 315
             ++S   ++ YCG+
Sbjct: 309 LKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895877.11.5e-17590.27probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
XP_038893580.11.6e-17490.21probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
TYJ99087.13.9e-16887.50putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa][more]
XP_031738134.15.1e-16886.45probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571... [more]
XP_004151475.36.7e-16886.14probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570... [more]
Match NameE-valueIdentityDescription
Q945B61.9e-7241.40Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA31.6e-7141.27Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z57.4e-6941.45Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ363.5e-6340.52Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q9ZTA12.7e-5532.522-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A5D3BIZ71.9e-16887.50Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1C7121.3e-15380.42probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara... [more]
A0A6J1EAT71.8e-14276.09probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1E7C81.3e-14074.84probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1KFF78.3e-14074.84probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT1G52790.11.0e-8648.712-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.17.5e-8545.022-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.19.2e-8346.332-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.11.1e-7241.272-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.11.5e-6941.592-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..295
e-value: 2.7E-55
score: 190.2
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 171..264
e-value: 9.7E-19
score: 67.7
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 26..84
e-value: 9.6E-7
score: 29.6
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 5..313
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 5..313
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..301
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 161..267
score: 10.952338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC01G014340.1CcUC01G014340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding