CcUC01G007590 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC01G007590
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionProlyl-tRNA synthetase
LocationCicolChr01: 8117987 .. 8123104 (+)
RNA-Seq ExpressionCcUC01G007590
SyntenyCcUC01G007590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCTCCTGCAGAAGTTAAAAACTGAAAACATGGCCGGTCCTAAGCCTGGTAGCTCTACTACTAATCCTAAAGCTGGTAAATATTGTTTCTTTAATACTGTAGTTGTTTTTGCAGTTGTTTCATACTCCCTAGTTTGGTTTGTGTTTACTAGTTCTTTGCTGTTTACTGGTTTTTGCAGGTGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTAGGTCTCACTAATAAGAAGGACGATAATTTTGGAGAGTGGTATTCTGAGGTGTGCTTAATTTTTGTTTCTATCCATTTGAAAATGACTTTTTCTGTTCGGTAAAATTATTCGTATGAGTTTGACAATGTTGTTTGACGGCTGTTGTAGTGAAGGAAGGTAAACCGTGTAGTTTCACTCCAAGGCTGTCAAATTACATGTAGCAAACCATGTGTAAAATTATTAGTATGATTTGAGTTTTTTTTTTCTCTCTCATCGCACTTTTACTTGAAAAGGCTTGAAGCTCGAACTGTTTTCCTTTAACTACGAAGAGAAGTGTTTTTTTATTGGTTCATAAGGTTTTATTAATTTTTAGAACTTCTATTCGGCATTTTGTATTTTTGTTGCAAAATGGCATTGGTTAATGAAATAGTTTATTATGTGAAACTAACAAAAAGGAGGAAAAAATTTTAAAGTACGTCTTTTTGGAGGAAGCTCCCCAGTAATATTATGAACAGGCTTTTTCGGTGAGTTGAATAATTCTTTTTCTAGTTTGTCTAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATTTCTGGCTGTTATATACTGAGGCCGTGGGCAATATCTGTCTGGGAGACTATGCAAGTAAGGATGCTACTCTTTCTATTGTCAGTTTTACAATCTGTCGTCTTGAAGTTCAAAGATTTAATTTGTCATCGTGCAGGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCCGAGGTGATGTTTTATTATTGATATATGCAGCAATAGAGAAACTTCTCCATCTGAATTTAAAATGCTTACTATCTTTGTTTGAAGTTACTTATTATTAATATTTTTTAATTTATGAGAAATGGGCGTTTCATTTATATGATGAAGTTTGAAATGCTTACTATCTTTGTTGGGTGTAATACCAGTTAAGTGATGTTATGGTTGTTATGGTCATTGGCTTATGAATATCATTTCAATTTTTGTGATATTTTGATTGTACCGAGTGTCTCTCAGTGTATTGGTTCTATGTCCCCAGTTAGTTCCTCTAGCCTCTACCTGTTACTTATCGGCATAGCTTTGGCTCAAGCAATCAATTTTTATTGCTTGTTATTAAAAGCTATACTTGATAAGATTGTTGACACCCAGCCCCTTCCTCTTTTGTACTGCAGTTCAGTAAGAATGTATTCAATGATGAGTAATCTTTCTTGGCATTTCATGTTTGTTTTTAAGCAATCTTCCCATTTCTTATGTTTTGTTGTTTCATTTTGAATTTTGAAGCTGATAAAGGTGCAATTGTGGTTTTATGCTGATGGTTTGGTACATGGCATTGATTTGCAGGTCGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGTCCTACAAGTGAGACTGTTATGTATCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACCTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGCAATTACTTTTACTTGTGCTGATCTTTTTGTTTCTTTTCTTTTCACTACGTTGATGGACTAAGTTGAACTCGTTAACAGTTGGGTGTTTTATAATTTGTTCAATGTTCTTAAGTAATTATCTTTTTTGCACAAGTCAATATAATCTTCTTGAACCTTATAAGTAATTTTAATCTTCATCGTTTCCAAATTATTTTAAACCATTTGGAGAAGAAGACTTATTTTATGAACTACTGATTGCAGGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTATCGTATTCTAGGATTTTAAATTTTGATCTAGAAAAATGTTTTTGCTATGGTTAGCTGTAGGGTTTGAAACCAAGTTATCTTTTTCTCAGTTTCCAGTGATTAAATGAGCCAAATATTTTTGGGATTAGTGAATTGAAGTTGAATCTATCTTTGACATACAGTTTCACTTTTGGGTTGACTTCTGTGTAAAAATTAAAGAAGTTTGTAGATTTAAGCATCCTGTTCAACGGCAATTTCTATTTGAATAATAAAGAATTTTTTAGAACATAATAAACTTGTTGGTTTGTTTGATGTCACAAGAAAGTGCTGTGGAGTCTTAAGTTAACTGTGAAGGTGTAGTACATCTTTTAGAACTTTTGAGATGTAGGGTCACCACTCACCACAGAGACAATGCTACTATAATTATAAAATTTATTATCTCGAGCCAATTTGGAGATTCAAACATGCAAAACTCACTTTGATTGGAAGCAAAAAGAATGAGTGGGGTCTATATTGGCATCCTCCAGTTCTTGAAGTGATCATCCTTAAGTGCGTGCTTTGGACTCAAGGTTGAGTCATGCGTTTCCTTCAGTTGGATAGCTTGATAGTGCAATTTAGCTTTCATTTGCCTCATAAATTTTGGATTTTATATATTATTGGATAACTTTTAACCGTCAAAACTGTTTTTTCCCTTTAAGTTTGGAGACTGTTAATGAATTCTTTAGGGTTCTTTTCAAAGAATTATGTTTCTTTTTTTTTTTTTTTTTTCCCTTTTTTTCCTTTTTTGCCATATGTTAAGTTTAATCGATATTTTGCATGATGAACCATGTTCCATTGTTCATAGATGAACTAATGAATGATAGCTGTTATGCTTCTGATTTCTCTCTCTTTCCTTTTCTTCTTTGGGTGGAAAACAAAGTTATATAAACAATTGAAAGAATATAAAGGAGAGAGGGAAGCTTAAATCTCCATCTTATTCTTAACGAAGCCAAGGGAGATCACCAAAATAGCCTTCAATCTAACCCTGAAATATGCAGTTCTTTTGTGTACCTGTGGTCAATATCTAAAGGTCACTTGTATTTCAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTAGCGATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGTATAAGTTGACTAAATGTTCTGACCTGCTTTCAGCATGGCGAATTAATATCTGTACTGGTTTTAAGTTGTATAAAAACCATTTCTTCCTTTGTGATATGCAGGCATTTATCCCCAACACTGGTCGTGGAATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAGATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAGAATTCGTGGGCCTATAGTACCAGAACGGTAATATTTTGATACTTCCATTTGTCTTCGCCTTTGGTAATTGTCAGAGTGAAATTCTGCTTCTTGTTAATCCAGCCTTCTAAACTGTTTGCAGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACCTTAGGGATAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTTAGATATTCTCTTACTAATGTTATTTTCATTGCTACTGAAATACTGTGTTCCCCTCGCTAATACTTGTTAATGATTTGGATTGGGTTGATACATGGTGCTAATATATAATTATATACTTTGTTTTCTAGGTACGTGCAGTTCGCCGTGATAATTCTGGAAAGAAGGACATACCTAGGGCTTCGTTGGTTGAACAAGTGAAAGGATTGCTAGAAAGTATTCAACAAAGCCTGTTTTATGCAGCAAAAGAAAAACGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGTAGAGCTTCAGTGTTTCTTGTCGCTGTTTTGATTCTACTTGTGTTCTTTATTATGTATAATTTGATTGTACTAGTGGACTGGCTACTTTTTGGTTCTGAATTTTCATGAGGCCACAATAATTTTATTTGCTTAGTTGAAGTGATCCTGGTGGTTATTGTTTTCCAGTAGTCAATACGCTTTCGTATGATGAAATCTATTTGCAGAATTAACTGTAAAATCCTGGTTTGTTGTTGAGTTTGTTTTTTGTGGAGCTTGTTTTTGTAGCTAGGAAGGAAGATTTTGTTGCCATCTTGGCTGCTGCAGCTTAAATAATGTTCATTAGGATGCTCACTGGCTTGGGTCAATGCGTTTTCGAGTATTTAGTTTCTATTAAAGTCTGTGGTAGTTGTGGAATAAATCGAGAGAATTTTTCTGAAATATGAAATATGTAATATATCTGTTCTATTCCAACTTCTGTTATTTACTTTGTATATATATATATATATTTCTTTCTTCTAAAGGATGATGGTTTCATTGTCATGTTCCTCCAGGAGGTTGAGAAGGATGTAAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCTTATGTTCCCCGTTTGAGCAGCCCGCCCTTCCAGAAGGTTACTAAATTCGTGTCCAATCTGTATTCATTTTACCTTTTTGATAATAGGAGTTTATCTTGTGGCATCTTGGGAACTTATTAGCAATTCCTTGAATTACTACGATGTTTGTGCTCAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

mRNA sequence

ATGATTCTCCTGCAGAAGTTAAAAACTGAAAACATGGCCGGTCCTAAGCCTGGTAGCTCTACTACTAATCCTAAAGCTGGTGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTAGGTCTCACTAATAAGAAGGACGATAATTTTGGAGAGTGGTATTCTGAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATTTCTGGCTGTTATATACTGAGGCCGTGGGCAATATCTGTCTGGGAGACTATGCAAGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCCGAGTGTATTGGTTCTATGTCCCCAGTTAGTTCCTCTAGCCTCTACCTGTTACTTATCGGCATAGCTTTGGCTCAAGCAATCAATTTTTATTGCTTGTTATTAAAAGCTATACTTGATAAGATTGTTGACACCCAGCCCCTTCCTCTTTTGTACTGCAGTTCAGTCGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGTCCTACAAGTGAGACTGTTATGTATCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACCTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGCAATTACTTTTACTTGTGCTGATCTTTTTGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTAGCGATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCCAACACTGGTCGTGGAATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAGATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAGAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACCTTAGGGATAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTACGTGCAGTTCGCCGTGATAATTCTGGAAAGAAGGACATACCTAGGGCTTCGTTGGTTGAACAAGTGAAAGGATTGCTAGAAAGTATTCAACAAAGCCTGTTTTATGCAGCAAAAGAAAAACGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTTGAGAAGGATGTAAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCTTATGTTCCCCGTTTGAGCAGCCCGCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

Coding sequence (CDS)

ATGATTCTCCTGCAGAAGTTAAAAACTGAAAACATGGCCGGTCCTAAGCCTGGTAGCTCTACTACTAATCCTAAAGCTGGTGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTAGGTCTCACTAATAAGAAGGACGATAATTTTGGAGAGTGGTATTCTGAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATTTCTGGCTGTTATATACTGAGGCCGTGGGCAATATCTGTCTGGGAGACTATGCAAGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCCGAGTGTATTGGTTCTATGTCCCCAGTTAGTTCCTCTAGCCTCTACCTGTTACTTATCGGCATAGCTTTGGCTCAAGCAATCAATTTTTATTGCTTGTTATTAAAAGCTATACTTGATAAGATTGTTGACACCCAGCCCCTTCCTCTTTTGTACTGCAGTTCAGTCGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGTCCTACAAGTGAGACTGTTATGTATCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACCTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGCAATTACTTTTACTTGTGCTGATCTTTTTGAGTCGTGAGTTCCTTTGGCAGGAAGGGCATACTGCTTTTGCTACCAAGGATGAAGCAGATACAGAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATACCTAGCGATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCCAACACTGGTCGTGGAATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAAATGTTTGAGATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAGAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGCTGCCTCCCAAAGTTGCATCAGTTCAAGTCATTATAGTTCCTGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACCTTAGGGATAACTATTCTCCTGGATGGAAGTACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCTAAGGACTTGGCAAACAATCAGGTACGTGCAGTTCGCCGTGATAATTCTGGAAAGAAGGACATACCTAGGGCTTCGTTGGTTGAACAAGTGAAAGGATTGCTAGAAAGTATTCAACAAAGCCTGTTTTATGCAGCAAAAGAAAAACGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTTGAGAAGGATGTAAAAACAAGGACTAAAGGTGAGATGGGAGCAGCGAAAACCTTATGTTCCCCGTTTGAGCAGCCCGCCCTTCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGCCGAAGCTACTAA

Protein sequence

MILLQKLKTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Homology
BLAST of CcUC01G007590 vs. NCBI nr
Match: XP_038874652.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.8e-291
Identity = 504/574 (87.80%), Postives = 507/574 (88.33%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVK LL+SIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKELLDSIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAKEKRDACI+VINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKEKRDACIKVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 511

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPF+QP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFQQPDLPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CcUC01G007590 vs. NCBI nr
Match: KAG7032618.1 (Proline--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1009.6 bits (2609), Expect = 1.1e-290
Identity = 499/574 (86.93%), Postives = 505/574 (87.98%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS   PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQ+L  V  F 
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQVLFRVFFFS 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRASLVEQVK LL+SIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 521

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 521

BLAST of CcUC01G007590 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.5e-289
Identity = 501/574 (87.28%), Postives = 503/574 (87.63%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS TNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWAIS+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYR IYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRCIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLS+AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR SLVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAKEKRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 511

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CcUC01G007590 vs. NCBI nr
Match: KAG6601922.1 (Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.3e-288
Identity = 499/580 (86.03%), Postives = 504/580 (86.90%), Query Frame = 0

Query: 6    KLKTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEY 65
            KLKT  MAGPKPGSS   PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEY
Sbjct: 610  KLKTLRMAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEY 669

Query: 66   YDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 125
            YDISGCYILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE
Sbjct: 670  YDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 729

Query: 126  CIGSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKS 185
                                                                 VAWVTKS
Sbjct: 730  -----------------------------------------------------VAWVTKS 789

Query: 186  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLL 245
            GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR    
Sbjct: 790  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---- 849

Query: 246  LVLIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFA 305
                  SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFA
Sbjct: 850  ------SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFA 909

Query: 306  GGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRT 365
            GGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRT
Sbjct: 910  GGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRT 969

Query: 366  IGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEV 425
            IGVMVMVHGDDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLS+AGIRAEV
Sbjct: 970  IGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEV 1029

Query: 426  DLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKG 485
            D RDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRASLVEQVK 
Sbjct: 1030 DARDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKE 1089

Query: 486  LLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG 545
            LL+SIQQSLF AAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG
Sbjct: 1090 LLDSIQQSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG 1126

Query: 546  EMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
            EMGAAKTLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 1150 EMGAAKTLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 1126

BLAST of CcUC01G007590 vs. NCBI nr
Match: XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1000.0 bits (2584), Expect = 8.7e-288
Identity = 500/574 (87.11%), Postives = 503/574 (87.63%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS T PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRASLVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 510

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CcUC01G007590 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 8.3e-249
Identity = 418/581 (71.94%), Postives = 469/581 (80.72%), Query Frame = 0

Query: 5   QKLKTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
           +K+K E +   K   +++   +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIE
Sbjct: 17  EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76

Query: 65  YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
           YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77  YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136

Query: 125 ECIGSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTK 184
           E                                                     VAWVTK
Sbjct: 137 E-----------------------------------------------------VAWVTK 196

Query: 185 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 244
           SG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIR   
Sbjct: 197 SGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIR--- 256

Query: 245 LLVLIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKF 304
                  SREFLWQEGHTAFATK EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKF
Sbjct: 257 -------SREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKF 316

Query: 305 AGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTR 364
           AGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTR
Sbjct: 317 AGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTR 376

Query: 365 TIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAE 424
           TIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+TQGI+DAC+AT   L +AGIRAE
Sbjct: 377 TIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAE 436

Query: 425 VDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVK 484
            DLRDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR SLVE VK
Sbjct: 437 EDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVK 496

Query: 485 GLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTK 544
            LLE IQQ+++  AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTK
Sbjct: 497 ELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTK 530

Query: 545 GEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           GE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 GETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

BLAST of CcUC01G007590 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.7e-156
Identity = 296/607 (48.76%), Postives = 370/607 (60.96%), Query Frame = 0

Query: 17  PGSSTTNPKAGGKKKE---------VKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD 76
           PGS+T   K   +KK+         ++    +G+T +KD +F  WY +V+   +MIEYYD
Sbjct: 174 PGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEYYD 233

Query: 77  ISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECI 136
           ISGCYIL+PW+ S+WE +Q +FD EIKK+ ++N YFPLFVS  VL++EKDH+EGFAPE  
Sbjct: 234 ISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPE-- 293

Query: 137 GSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGE 196
                                                              VAWVT++G 
Sbjct: 294 ---------------------------------------------------VAWVTRAGT 353

Query: 197 SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLV 256
           S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF +P PF+R      
Sbjct: 354 SELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQPFLR------ 413

Query: 257 LIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGG 316
               +REFLWQEGHTA  T + A  EV +IL+LY RIY + LA+PVIKG KSE EKFAGG
Sbjct: 414 ----TREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGG 473

Query: 317 LYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAMVWQNSW 376
           ++TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  VWQNSW
Sbjct: 474 MFTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSW 533

Query: 377 AYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPV----PYKDADTQGIFDACSATLDT 436
             STRTIGV VMVHGDDKGL LPP +A VQ ++VP        D +   I   CS   D 
Sbjct: 534 GLSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFCSKLADR 593

Query: 437 LSKAGIRAEVDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIP 496
           L+ A IR E DLR  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD   K  +P
Sbjct: 594 LNAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFEKIPVP 653

Query: 497 RASLVEQVKGLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEV 556
             +L + V  LL  IQ +++  AK +RDA +  +  W +F  AL++K +++ PWC+  E 
Sbjct: 654 LNNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPWCNTTEC 713

Query: 557 EKDVKTRT------------KGEMGAAKTLCSPFEQP----ALPEG-TKCFASGKPAKKW 586
           EK++K  +            K     AK+LC P EQP    A+ EG TKC   G  AK W
Sbjct: 714 EKEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCGNLAKVW 716

BLAST of CcUC01G007590 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 550.1 bits (1416), Expect = 3.1e-155
Identity = 291/595 (48.91%), Postives = 373/595 (62.69%), Query Frame = 0

Query: 8    KTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD 67
            K    A PKP        A      VKK+T LGL   K+DN  +WYS+V+  GEMIEYYD
Sbjct: 1184 KAPKEAQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYD 1243

Query: 68   ISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECI 127
            +SGCYILR W+ ++W+ ++ +FDAEI +M +K CYFP+FVS  VL++EK HI  FAPE  
Sbjct: 1244 VSGCYILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPE-- 1303

Query: 128  GSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGE 187
                                                               VAWVTKSG+
Sbjct: 1304 ---------------------------------------------------VAWVTKSGD 1363

Query: 188  SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLV 247
            SDL  PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF  PTPF+R      
Sbjct: 1364 SDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFLR------ 1423

Query: 248  LIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGG 307
                +REFLWQEGHTAFA K+EA  EVL+IL+LY  +Y   LAIPV+KG+K+E EKFAGG
Sbjct: 1424 ----TREFLWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGG 1483

Query: 308  LYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWAYSTRTI 367
             YTT+VEAFI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW  +TRTI
Sbjct: 1484 DYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTI 1543

Query: 368  GVMVMVHGDDKGLVLPPKVASVQVIIVP----VPYKDADTQGIFDACSATLDTLSKAGIR 427
            GVM+MVH D++GLVLPP VA +Q I+VP    V  KD +   + DAC A    L   G+R
Sbjct: 1544 GVMIMVHADNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVR 1603

Query: 428  AEVDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQ 487
             E D RDNYSPGWK++HWE+KGVPLR+E+GPKDL   Q+ AVRRD   K  IP A + ++
Sbjct: 1604 CEGDYRDNYSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKK 1663

Query: 488  VKGLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTR 547
            +  LLE+I +S+   A+E   +  + +  W +F   L QK ++LAP+C E   E  +K  
Sbjct: 1664 IPALLETIHESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKAD 1714

Query: 548  T----KGEMGA----AKTLCSPFEQPA-LPEGTKCF---ASGKPAKKWSYWGRSY 586
            +    + E GA    AK+LC PF+QPA +    KC     + KP K ++ +GRSY
Sbjct: 1724 SARGEEAEPGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

BLAST of CcUC01G007590 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 547.7 bits (1410), Expect = 1.6e-154
Identity = 282/573 (49.21%), Postives = 364/573 (63.53%), Query Frame = 0

Query: 28   GKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAISVWETMQV 87
            G+ +  KK+T LGL  KK++N  +WYS+V+   EMIEY+DISGCYILRPWA ++WE ++ 
Sbjct: 1003 GEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKD 1062

Query: 88   FFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMSPVSSSSLYLLLIGIAL 147
            FFDAEIKK+ ++NCYFP+FVS   L++EK H+  FAPE                      
Sbjct: 1063 FFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPE---------------------- 1122

Query: 148  AQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLEVPIAIRPTSETVMYPY 207
                                           VAWVT+SG+++L  PIAIRPTSETVMYP 
Sbjct: 1123 -------------------------------VAWVTRSGKTELAEPIAIRPTSETVMYPA 1182

Query: 208  YSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFLSREFLWQEGHTAFATK 267
            Y+KW++ HRDLP+KLNQWCNVVRWEF HP PF+R          +REFLWQEGH+AFAT 
Sbjct: 1183 YAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLR----------TREFLWQEGHSAFATM 1242

Query: 268  DEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGA 327
            +EA  EVL+IL+LY ++YEE LAIPV+KG+K+E EKFAGG YTT++EAFI  +GR IQG 
Sbjct: 1243 EEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGG 1302

Query: 328  TSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKV 387
            TSH LGQNF+KMFEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GLVLPP+V
Sbjct: 1303 TSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRV 1362

Query: 388  ASVQVIIVPVPYKDA----DTQGIFDACSATLDTLSKAGIRAEVDLRDNYSPGWKYSHWE 447
            A VQV+I+P    +A    D + +   C+     L    IR   DLRDNYSPGWK++HWE
Sbjct: 1363 ACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWE 1422

Query: 448  MKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQQSLFYAAKEK 507
            +KGVP+R+E+GP+D+ + Q  AVRRD   K  +       +++ +LE IQ +LF  A E 
Sbjct: 1423 LKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASED 1482

Query: 508  RDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA----AKTL 567
                + V NT E+F + L   K++  P+C E + E  +K  T      E GA    AK+L
Sbjct: 1483 LKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSL 1512

Query: 568  CSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 586
            C PF+    L  G KC     PAK ++ +GRSY
Sbjct: 1543 CIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512

BLAST of CcUC01G007590 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 547.0 bits (1408), Expect = 2.6e-154
Identity = 289/585 (49.40%), Postives = 370/585 (63.25%), Query Frame = 0

Query: 16   KPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILR 75
            K   S  +    G+ +  KK+T LGL  KK++N  EWYS+V+   EMIEYYD+SGCYILR
Sbjct: 991  KSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILR 1050

Query: 76   PWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMSPVSS 135
            PW+ S+WE+++ FFDAEIKK+ ++NCYFP+FVS   L++EK+HIE FAPE          
Sbjct: 1051 PWSYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPE---------- 1110

Query: 136  SSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLEVPIA 195
                                                       VAWVT+SG+++L  PIA
Sbjct: 1111 -------------------------------------------VAWVTRSGKTELAEPIA 1170

Query: 196  IRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFLSREF 255
            IRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF HP PF+R          +REF
Sbjct: 1171 IRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLR----------TREF 1230

Query: 256  LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 315
            LWQEGH+AFAT +EA  EVL+ILELY R+YEE LAIPV++G+K+E EKFAGG YTT++EA
Sbjct: 1231 LWQEGHSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEA 1290

Query: 316  FIPNTGRGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVH 375
            FI  +GR IQGATSH LGQNF+KM EI FE+ K  GEK   +Q SW  +TRTIGVMVMVH
Sbjct: 1291 FISASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVH 1350

Query: 376  GDDKGLVLPPKVASVQVIIVPVPYKDA----DTQGIFDACSATLDTLSKAGIRAEVDLRD 435
            GD+ GLVLPP+VASVQV+++P    +A    D + +   C+     L  A IR  VDLRD
Sbjct: 1351 GDNMGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRD 1410

Query: 436  NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLES 495
            NYSPGWK++HWE+KGVP+R+E+GP+D+ + Q  AVRRD   K  I       +++ +LE 
Sbjct: 1411 NYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLED 1470

Query: 496  IQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG---- 555
            IQ +LF  A E     + V NT E+F + L   K+   P+C E + E  +K  T      
Sbjct: 1471 IQLNLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDV 1512

Query: 556  EMGA----AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 586
            E GA    AK+LC PF     L  G  C     PAK ++ +GRSY
Sbjct: 1531 EPGAPSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512

BLAST of CcUC01G007590 vs. ExPASy TrEMBL
Match: A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.2e-288
Identity = 500/574 (87.11%), Postives = 503/574 (87.63%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS T PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRASLVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 510

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CcUC01G007590 vs. ExPASy TrEMBL
Match: A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.5e-288
Identity = 499/574 (86.93%), Postives = 503/574 (87.63%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS T PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRA+LVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 510

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CcUC01G007590 vs. ExPASy TrEMBL
Match: A0A5A7TN35 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003510 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.1e-287
Identity = 497/574 (86.59%), Postives = 503/574 (87.63%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS TN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWAIS+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL+L     
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLML----- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
              FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 ---FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTL+ AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR SLVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 513

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFDQPALPEGTKCFASGKPAKKWSYWGRSY 513

BLAST of CcUC01G007590 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.1e-287
Identity = 497/574 (86.59%), Postives = 501/574 (87.28%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS TN KAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWAIS+WETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTL+ AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR SLVEQVK LLESIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 511

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CcUC01G007590 vs. ExPASy TrEMBL
Match: A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.7e-287
Identity = 495/574 (86.24%), Postives = 500/574 (87.11%), Query Frame = 0

Query: 12  MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 71
           MAGPKPGSS   PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 72  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMS 131
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE      
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE------ 120

Query: 132 PVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLE 191
                                                          VAWVTKSGESDLE
Sbjct: 121 -----------------------------------------------VAWVTKSGESDLE 180

Query: 192 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFL 251
           VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR          
Sbjct: 181 VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIR---------- 240

Query: 252 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 311
           SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT
Sbjct: 241 SREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTT 300

Query: 312 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 371
           SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM
Sbjct: 301 SVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVM 360

Query: 372 VHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNY 431
           VHGDDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVD RDNY
Sbjct: 361 VHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNY 420

Query: 432 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLESIQ 491
           SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRASLVEQVK LL+SIQ
Sbjct: 421 SPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQ 480

Query: 492 QSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 551
           QSLF AAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK
Sbjct: 481 QSLFDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAK 511

Query: 552 TLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           TLCSPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 541 TLCSPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CcUC01G007590 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 860.9 bits (2223), Expect = 5.9e-250
Identity = 418/581 (71.94%), Postives = 469/581 (80.72%), Query Frame = 0

Query: 5   QKLKTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
           +K+K E +   K   +++   +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIE
Sbjct: 17  EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76

Query: 65  YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
           YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77  YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136

Query: 125 ECIGSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTK 184
           E                                                     VAWVTK
Sbjct: 137 E-----------------------------------------------------VAWVTK 196

Query: 185 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 244
           SG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIR   
Sbjct: 197 SGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIR--- 256

Query: 245 LLVLIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKF 304
                  SREFLWQEGHTAFATK EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKF
Sbjct: 257 -------SREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKF 316

Query: 305 AGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTR 364
           AGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTR
Sbjct: 317 AGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTR 376

Query: 365 TIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAE 424
           TIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+TQGI+DAC+AT   L +AGIRAE
Sbjct: 377 TIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAE 436

Query: 425 VDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVK 484
            DLRDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR SLVE VK
Sbjct: 437 EDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVK 496

Query: 485 GLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTK 544
            LLE IQQ+++  AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTK
Sbjct: 497 ELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTK 530

Query: 545 GEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           GE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 GETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

BLAST of CcUC01G007590 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 860.9 bits (2223), Expect = 5.9e-250
Identity = 418/581 (71.94%), Postives = 469/581 (80.72%), Query Frame = 0

Query: 5   QKLKTENMAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIE 64
           +K+K E +   K   +++   +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIE
Sbjct: 17  EKVKKEKVVKEKVAKASS---SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIE 76

Query: 65  YYDISGCYILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAP 124
           YYDISGCYILRPW++++WE MQ+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAP
Sbjct: 77  YYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAP 136

Query: 125 ECIGSMSPVSSSSLYLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTK 184
           E                                                     VAWVTK
Sbjct: 137 E-----------------------------------------------------VAWVTK 196

Query: 185 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLL 244
           SG+SDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIR   
Sbjct: 197 SGKSDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIR--- 256

Query: 245 LLVLIFLSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKF 304
                  SREFLWQEGHTAFATK EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKF
Sbjct: 257 -------SREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKF 316

Query: 305 AGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTR 364
           AGGLYTTSVEAFIPNTGRG+QGATSHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTR
Sbjct: 317 AGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTR 376

Query: 365 TIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAE 424
           TIGVM+M HGDDKGLVLPPKVASVQV+++PVPYKDA+TQGI+DAC+AT   L +AGIRAE
Sbjct: 377 TIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAE 436

Query: 425 VDLRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVK 484
            DLRDNYSPGWKYS WEMKGVPLRIEIGP+DL N+QVR VRRDN  K+DIPR SLVE VK
Sbjct: 437 EDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVK 496

Query: 485 GLLESIQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTK 544
            LLE IQQ+++  AK+KR+AC+Q + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTK
Sbjct: 497 ELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTK 530

Query: 545 GEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
           GE GAAKTLCSPF+QP LPEGT CFASGKPAKKW+YWGRSY
Sbjct: 557 GETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

BLAST of CcUC01G007590 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.5e-108
Identity = 214/574 (37.28%), Postives = 312/574 (54.36%), Query Frame = 0

Query: 19  SSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 78
           S T +P+   K  EV +         +  +F  WY +V+ S E+ +Y  + G  ++RP+ 
Sbjct: 45  SGTASPET--KSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYG 104

Query: 79  ISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPECIGSMSPVSSSSL 138
            ++WE +Q + + + K+    N YFP F+    +++E  H+EGF+PE             
Sbjct: 105 YAIWEAIQDYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPE------------- 164

Query: 139 YLLLIGIALAQAINFYCLLLKAILDKIVDTQPLPLLYCSSVAWVTKSGESDLEVPIAIRP 198
                                                   +A VT  G  +LE  + +RP
Sbjct: 165 ----------------------------------------LALVTVGGGKELEEKLVVRP 224

Query: 199 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRQLLLLVLIFLSREFLWQ 258
           TSET++   +++WI  +RDLPL +NQW NV RWE     PFIR L          EFLWQ
Sbjct: 225 TSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTL----------EFLWQ 284

Query: 259 EGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIP 318
           EGHTA AT +EA+ E  +++E+Y R   E  AIPVI G+KS++E FAG   T ++EA + 
Sbjct: 285 EGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMG 344

Query: 319 NTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKG 378
           +  + +Q  TSH LGQNF++ F   F +E GE+  VWQ SWA STR +G ++M HGDD G
Sbjct: 345 DR-KALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTG 404

Query: 379 LVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDLRDNYSPGWKYS 438
           L+LPPK+A +QV+IVP+  KD +  G+  A S+  + L  AG+R ++D  D  +PGWK++
Sbjct: 405 LMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFN 464

Query: 439 HWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKK------DIPRASLVEQVKGLLESIQQ 498
            WEMKG+PLRIEIGP+D+++N V   RRD  GK        +  ++LV  VK  L+ IQ 
Sbjct: 465 FWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDEIQT 524

Query: 499 SLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKT 558
           SL   A   RD+ I  +N++ E  +A+S  K    PW   +  E+ VK  T      A  
Sbjct: 525 SLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEET-----GATI 543

Query: 559 LCSPFEQPALPEGTK-CFASGKPAKKWSYWGRSY 586
            C PFEQ    +GTK C  +G PA++ + + +SY
Sbjct: 585 RCFPFEQ---TQGTKTCLMTGNPAEEVAIFAKSY 543

BLAST of CcUC01G007590 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 290.4 bits (742), Expect = 3.2e-78
Identity = 144/221 (65.16%), Postives = 165/221 (74.66%), Query Frame = 0

Query: 371 MVHGDDKGLVLPPKVASVQVIIVPVPYKD-ADTQGIFDACSATLDTLSKAGIRAEVDLRD 430
           M HGDDKGLV PPKVA VQV+++ VP K  AD Q + DAC A   TL  AGIRAE D+RD
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 431 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKGLLES 490
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR V RDN  K D+ R  L+EQVK LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 491 IQQSLFYAAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEM-- 550
           IQ +L+  AK K + C Q + TW+EF EALSQKK+ILAPWCD+ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 551 ---GAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 586
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309


HSP 2 Score: 63.2 bits (152), Expect = 8.2e-10
Identity = 28/47 (59.57%), Postives = 37/47 (78.72%), Query Frame = 0

Query: 35 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAIS 81
          KET LG+T KKD++FG+WYSE    GE++EYY+ + GCYIL+P  IS
Sbjct: 3  KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874652.13.8e-29187.80proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida][more]
KAG7032618.11.1e-29086.93Proline--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038907011.13.5e-28987.28proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907... [more]
KAG6601922.11.3e-28886.03Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022979761.18.7e-28887.11proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M1R28.3e-24971.94Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... [more]
O601553.7e-15648.76Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P286683.1e-15548.91Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G... [more]
P078141.6e-15449.21Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=... [more]
Q8CGC72.6e-15449.40Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1IRP04.2e-28887.11Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1[more]
A0A6J1GUJ55.5e-28886.93Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1[more]
A0A5A7TN352.1e-28786.59Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... [more]
A0A0A0KTZ12.1e-28786.59Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1[more]
A0A6J1GYJ14.7e-28786.24Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G62120.15.9e-25071.94Class II aaRS and biotin synthetases superfamily protein [more]
AT3G62120.25.9e-25071.94Class II aaRS and biotin synthetases superfamily protein [more]
AT5G52520.16.5e-10837.28Class II aaRS and biotin synthetases superfamily protein [more]
AT5G10880.13.2e-7865.16tRNA synthetase-related / tRNA ligase-related [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016061Proline-tRNA ligase, class II, C-terminalSMARTSM00946ProRS_C_1_2coord: 512..585
e-value: 2.2E-28
score: 110.3
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 512..585
e-value: 3.9E-26
score: 91.1
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 506..585
e-value: 2.4E-28
score: 100.2
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 507..585
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeTIGRFAMTIGR00408TIGR00408coord: 178..585
e-value: 3.0E-141
score: 469.5
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 22..127
coord: 175..585
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeHAMAPMF_01571Pro_tRNA_synth_type3coord: 42..585
score: 35.948776
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 42..382
e-value: 4.5E-100
score: 336.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..37
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 22..127
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 175..585
NoneNo IPR availableCDDcd00862ProRS_anticodon_zinccoord: 378..585
e-value: 3.84757E-94
score: 284.96
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 47..376
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 377..503
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 389..484
e-value: 2.6E-18
score: 66.0
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 194..370
e-value: 4.7E-13
score: 49.5
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 383..503
e-value: 1.7E-38
score: 133.1
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 172..382
score: 14.302841
IPR033721Prolyl-tRNA synthetase, catalytic domainCDDcd00778ProRS_core_arch_eukcoord: 48..372
e-value: 3.57865E-151
score: 433.175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC01G007590.1CcUC01G007590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006433 prolyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding