Carg28293 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28293
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mitotic spindle checkpoint protein MAD2
Location	Carg_Chr04: 13065348 .. 13065566 (-)
RNA-Seq Expression	Carg28293
Synteny	Carg28293

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTAGATGGTGGTCTGTCATGGGCAGATCAATGGGATTCCAACCCTGACCCAGGCCCTCCACCATCGTCGGCAGAAAACGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGGATGGGGGATCCTCCTCGTCAGAGAAGAGCATATTTAAGAAGGCAAGCTTGAAGTGGATAAAGGAATTGCGAAAGAAATCTGAGAAATCTTGA

mRNA sequence

ATGGGTTTAGATGGTGGTCTGTCATGGGCAGATCAATGGGATTCCAACCCTGACCCAGGCCCTCCACCATCGTCGGCAGAAAACGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGGATGGGGGATCCTCCTCGTCAGAGAAGAGCATATTTAAGAAGGCAAGCTTGAAGTGGATAAAGGAATTGCGAAAGAAATCTGAGAAATCTTGA

Coding sequence (CDS)

ATGGGTTTAGATGGTGGTCTGTCATGGGCAGATCAATGGGATTCCAACCCTGACCCAGGCCCTCCACCATCGTCGGCAGAAAACGGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGGATGGGGGATCCTCCTCGTCAGAGAAGAGCATATTTAAGAAGGCAAGCTTGAAGTGGATAAAGGAATTGCGAAAGAAATCTGAGAAATCTTGA

Protein sequence

MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLKWIKELRKKSEKS

Homology

[Close]

BLAST of Carg28293 vs. NCBI nr
Match: KAG6583660.1 (hypothetical protein SDJN03_19592, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032466.1 hypothetical protein SDJN02_06514, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.7 bits (335), Expect = 6.6e-28
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60
          MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK
Sbjct: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60

Query: 61 WIKELRKKSEKS 73
          WIKELRKKSEKS
Sbjct: 61 WIKELRKKSEKS 72

[Close]

BLAST of Carg28293 vs. NCBI nr
Match: XP_022932206.1 (uncharacterized protein LOC111438527 [Cucurbita moschata] >XP_023519855.1 uncharacterized protein LOC111783185 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 124.4 bits (311), Expect = 4.0e-25
Identity = 69/72 (95.83%), Postives = 70/72 (97.22%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60
          MGLDGGLSWADQWDSNPDPGPPPSSAENG  KKKKKKKKEKE+GGSSSSEKSIFKKASLK
Sbjct: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENG--KKKKKKKKEKEEGGSSSSEKSIFKKASLK 60

Query: 61 WIKELRKKSEKS 73
          WIKELRKKSEKS
Sbjct: 61 WIKELRKKSEKS 70

[Close]

BLAST of Carg28293 vs. NCBI nr
Match: XP_022970362.1 (uncharacterized protein LOC111469348 [Cucurbita maxima])

HSP 1 Score: 117.9 bits (294), Expect = 3.7e-23
Identity = 65/72 (90.28%), Postives = 66/72 (91.67%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60
          MGLDGGLSWADQWDSNPDPGPPPSS ENGKKKKKK     KE+GGSSSSEKSIFKKASLK
Sbjct: 1  MGLDGGLSWADQWDSNPDPGPPPSSTENGKKKKKK-----KEEGGSSSSEKSIFKKASLK 60

Query: 61 WIKELRKKSEKS 73
          WIKELRKKSEKS
Sbjct: 61 WIKELRKKSEKS 67

[Close]

BLAST of Carg28293 vs. NCBI nr
Match: KAG7012753.1 (Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.2 bits (256), Expect = 9.5e-19
Identity = 61/71 (85.92%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 4   DGGLSWADQWDSNPDPGPPPSSAENGKKKK-KKKKKKEKEDGG-SSSSEKSIFKKASLKW 63
           D GLSWADQWD+NPDP PPPSSAENGKKK   KKKKKEKE+GG SSSSEK+IFKKA+LKW
Sbjct: 206 DDGLSWADQWDNNPDPNPPPSSAENGKKKNIFKKKKKEKEEGGSSSSSEKNIFKKANLKW 265

Query: 64  IKELRKKSEKS 73
           IKELRKKSEKS
Sbjct: 266 IKELRKKSEKS 276

[Close]

BLAST of Carg28293 vs. NCBI nr
Match: XP_038892905.1 (uncharacterized protein LOC120081808 [Benincasa hispida])

HSP 1 Score: 72.0 bits (175), Expect = 2.3e-09
Identity = 48/74 (64.86%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASL- 60
          MGLDGGLSWADQWD+NPDP PP SS+EN KKK K        DG   SSEK  F+K  L 
Sbjct: 1  MGLDGGLSWADQWDNNPDP-PPSSSSENEKKKNK--------DG---SSEKGKFRKTILG 60

Query: 61 -KWIKELRKKSEKS 73
           KW+KELRKKS+KS
Sbjct: 61 FKWMKELRKKSDKS 62

[Close]

BLAST of Carg28293 vs. ExPASy TrEMBL
Match: A0A6J1EVQ9 (uncharacterized protein LOC111438527 OS=Cucurbita moschata OX=3662 GN=LOC111438527 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.9e-25
Identity = 69/72 (95.83%), Postives = 70/72 (97.22%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60
          MGLDGGLSWADQWDSNPDPGPPPSSAENG  KKKKKKKKEKE+GGSSSSEKSIFKKASLK
Sbjct: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENG--KKKKKKKKEKEEGGSSSSEKSIFKKASLK 60

Query: 61 WIKELRKKSEKS 73
          WIKELRKKSEKS
Sbjct: 61 WIKELRKKSEKS 70

[Close]

BLAST of Carg28293 vs. ExPASy TrEMBL
Match: A0A6J1HYV9 (uncharacterized protein LOC111469348 OS=Cucurbita maxima OX=3661 GN=LOC111469348 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.8e-23
Identity = 65/72 (90.28%), Postives = 66/72 (91.67%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLK 60
          MGLDGGLSWADQWDSNPDPGPPPSS ENGKKKKKK     KE+GGSSSSEKSIFKKASLK
Sbjct: 1  MGLDGGLSWADQWDSNPDPGPPPSSTENGKKKKKK-----KEEGGSSSSEKSIFKKASLK 60

Query: 61 WIKELRKKSEKS 73
          WIKELRKKSEKS
Sbjct: 61 WIKELRKKSEKS 67

[Close]

BLAST of Carg28293 vs. ExPASy TrEMBL
Match: A0A0A0KVC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590180 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-09
Identity = 48/74 (64.86%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASL- 60
          MGLDGGLSWADQWD NPD  PPPSS+EN KKK K        DG   SS+KS F+K  L 
Sbjct: 1  MGLDGGLSWADQWDYNPD--PPPSSSENEKKKNK--------DG---SSDKSKFRKTILG 60

Query: 61 -KWIKELRKKSEKS 73
           KW+KELRKKS+KS
Sbjct: 61 FKWMKELRKKSDKS 61

[Close]

BLAST of Carg28293 vs. ExPASy TrEMBL
Match: A0A1S3BE81 (uncharacterized protein LOC103488917 OS=Cucumis melo OX=3656 GN=LOC103488917 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.6e-09
Identity = 46/74 (62.16%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 1  MGLDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASL- 60
          MGLDGGLSWADQWD NPD  PPPSS++N KKK K        DG   SS+KS F+K  L 
Sbjct: 1  MGLDGGLSWADQWDYNPD--PPPSSSDNDKKKNK--------DG---SSDKSKFRKTILG 60

Query: 61 -KWIKELRKKSEKS 73
           KW+K+LRKKS+KS
Sbjct: 61 FKWMKDLRKKSDKS 61

[Close]

BLAST of Carg28293 vs. ExPASy TrEMBL
Match: A0A087HQU8 (Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA1G265100 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.0e-07
Identity = 42/70 (60.00%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 3  LDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLKWI 62
          +D GLSWADQWD N DP PP S+ E+ KKKKKK     KEDG  SS  K+I     LKW+
Sbjct: 1  MDSGLSWADQWDYNSDP-PPNSTNEDDKKKKKK-----KEDGSKSSFGKTIL---GLKWV 60

Query: 63 KELRKKSEKS 73
          KELRKKS+ S
Sbjct: 61 KELRKKSDNS 61

[Close]

BLAST of Carg28293 vs. TAIR 10
Match: AT1G67670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G24405.1); Has 18 Blast hits to 18 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 18; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 58.5 bits (140), Expect = 2.5e-09
Identity = 40/69 (57.97%), Postives = 47/69 (68.12%), Query Frame = 0


Query: 3  LDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLKWI 62
          +D G SWADQWD N +P P PSS +  + KKK KKKKE EDG  SS  K++      KW+
Sbjct: 1  MDSGPSWADQWDYN-NPDPLPSSTKEDESKKKNKKKKE-EDGSKSSLGKTLL---GFKWM 60

Query: 63 KELRKKSEK 72
          KELRKKSEK
Sbjct: 61 KELRKKSEK 64

[Close]

BLAST of Carg28293 vs. TAIR 10
Match: AT1G24405.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G67670.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-08
Identity = 41/69 (59.42%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 3  LDGGLSWADQWDSNPDPGPPPSSAENGKKKKKKKKKKEKEDGGSSSSEKSIFKKASLKWI 62
          +D GLSWADQWD N DP PP SS E+ KKKKKKKK   KEDG  S   K+I      KW+
Sbjct: 1  MDSGLSWADQWDYNSDP-PPNSSNEDDKKKKKKKK---KEDGSKSGIGKAIL---GFKWM 60

Query: 63 KELRKKSEK 72
          K L KKS+K
Sbjct: 61 K-LGKKSDK 61

The following BLAST results are available for this feature:

BLAST of Carg28293 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583660.1	6.6e-28	100.00	hypothetical protein SDJN03_19592, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022932206.1	4.0e-25	95.83	uncharacterized protein LOC111438527 [Cucurbita moschata] >XP_023519855.1 unchar...	[more]
XP_022970362.1	3.7e-23	90.28	uncharacterized protein LOC111469348 [Cucurbita maxima]	[more]
KAG7012753.1	9.5e-19	85.92	Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_038892905.1	2.3e-09	64.86	uncharacterized protein LOC120081808 [Benincasa hispida]	[more]

BLAST of Carg28293 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg28293 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVQ9	1.9e-25	95.83	uncharacterized protein LOC111438527 OS=Cucurbita moschata OX=3662 GN=LOC1114385...	[more]
A0A6J1HYV9	1.8e-23	90.28	uncharacterized protein LOC111469348 OS=Cucurbita maxima OX=3661 GN=LOC111469348...	[more]
A0A0A0KVC1	1.5e-09	64.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590180 PE=4 SV=1	[more]
A0A1S3BE81	5.6e-09	62.16	uncharacterized protein LOC103488917 OS=Cucumis melo OX=3656 GN=LOC103488917 PE=...	[more]
A0A087HQU8	4.0e-07	60.00	Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA1G265100 PE=3 SV=1	[more]

BLAST of Carg28293 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67670.1	2.5e-09	57.97	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G24405.1	2.1e-08	59.42	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..55
None	No IPR available	PANTHER	PTHR33386	OS02G0740600 PROTEIN	coord: 4..71
None	No IPR available	PANTHER	PTHR33386:SF1	F12A21.18-RELATED	coord: 4..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28293-RA	Carg28293-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009987	cellular process