Carg28253 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28253
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Carotenoid cleavage dioxygenase 7
Location	Carg_Chr17: 4065277 .. 4069301 (-)
RNA-Seq Expression	Carg28253
Synteny	Carg28253

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGCCAATCAACTCACTCCATCTCTCCGACCACAATTTCCGTCGCCGATCAAACTTCCTCCGGCGACCAGTATCCGATTTCCGCCTTCTCTAATTCCCAAAGCCATATCCATTCCCTCCCTGAACACTGGCCAGATATCGATCAATTCTCCTCCGTCGATCCCCACCGTTGATGATTCCATCGCAGCCTTCTGGGACTACCAGTTCCTCTTCGTTTCACAGCGGACCGAGACGGCGGACCCCACCGTCCTCCACCTGGTTGAAGGTGCCATCCCCGAAAATTTCCCTTCCGGCACCTACTACCTCGCCGGACCAGGCCTGTTCTCTGACGACCATGGCTCTACAGTACACCCCCTGGACGGCCACGGGTACCTCAGGGCATTCATTTTCGAGAACAAAAAGGAGGTCAGCTTCATGGCGAAGTACGTGAGAACGGAGGCGAAGATGGAGGAGCACGATCCTGCGACGGACACGTGGCGGTTCACGCATAGAGGGCCGTTCTCAGTGTTGAAGGGAGGGAGGAAGTTGGGGAATACGAAGGTTATGAAAAATGTGGCGAATACGAGTGTGCTGCAGTGGGGTGGCCACCTTTTGTGCCTATGGGAGGGCGGAGATCCGTACGAGATTCAGCCGGAGACTCTGGATACTGTCGGGAAGTTTTCAGGTGTTGACGGTGGCTGTGATCGTGATTCGCCATCCCAGGGCGGACGTCACGGTGCTGGGTTTTGGCGTTTTGCTGCGGAGCTTTTGAAGCCTGTTCTCTACGGTTGGTATTTAGTGATGCTAGTTTGAATTTTATTTCATAAAATTATAATAAATTAGTTTTATTTTAAACTTTATTAACATATATTATACTATTTAGATTTAATTTTTTAAAAATAGCCAACCACTCTTTCCCTCAGCCTCTCTAATTAAGGTGAGATCTCACAATTTATCCCATTCCGGATCATCATGGTTGCTAGCATTTTTCCCTTCTCCAATCAATGTGAGACCCTCCAATCTACCCTCTTTGAGGCTCAACGTCCTTCCTGGTTCATCACCTCATGTCCACCCTGGGGCTTAATCTTCTCATTGGCACATCGCCCGATGTCTTCTTCTAATACTATTTGTAATGCCCAAAGTTCACCACTAGCTGATATTGTTCTCTTTGGGTTTTCGTGTCTACCTCCTTCTAGGCTTAGTCTCCTCATTGGCACATCACCTAGTGTCTAGCTATGTTACCATTTGTAACTGCCAAATTCCACCACTAGTAAATATTATTATGTTTAGATTTTTTTTGAAAGTTGTTAAAAAGGGAAGAGAGGTTGAGGATTTCTAGGAGGATATTTTACAAATTTTTATTAAAAATTCTTTAAAAATAAATATAAAATTAGAATAAATATTTGAAATTAAACCATGGGTGGAAATAGGGATTAAAACCGGTAAAATAAGAAGATAATTATTATTAAAAACAATAGGAATTAGCAATAGAAATGGGGAGAGGAAAGCAGGTAAGAATAATATATAAAATAATTGGTTTTGAATTAAAATAATAATAATAATAATGGCGGAGTGGGCAAAATGGGAAATTAAAAAATAATAAAAAAGTAATTAAAAAATAGAATGTGGGAATAATGGGTACAGGAGTGTTCAAGATGCCACCAAGGAGGCTGTTGTCGCATTACAAGGTGGACGCTCAAAGGAATCGCCTCCTCTTAAACTCATGCAATGCTGAAGATATGCTTCTACCTACCAGTCATTTCACATTTTATGGTATAATATCTTTTTTTTTAAGAAAAATTTTATTTCTTATATATTTTGTTACCATTTATGACAACTTGATTTTCTTTGTGCAGAATTTGACAGTAATTTCAAGTTATTAGAGAAGAAAGAATTTGTGATCGATGATCACCTGATGATTCATGATTGGGCTTTTACCGATACTCATTACATCCTCTTCGCTAACAGAATAAAGCTTGATCTCTTCGGTATATTGTTCTTGTTCTTATTCTTATTCTTATTAATATTAATAATGATAATAATAATAATAATAAGCTACTATTGAAATTTTAATTTGAATAGATGACTGAAATAACTCAAATCAACTTAACTCAAATATTTTATAGTTGGATTCAGATGAATTCATTGTTTAATAAGAGTAGTATTGTAACCGCTAAAATTCAACGTTAGCAGATGTTGTTCTCTTTTGGACTTTCTCTTCTGGACTCCAAGTCCACTATCAGTATATATATTGTTTTCTTTTGTTTCAATACCACTATCAGTATATATATTGTTTTCTTTTGGGCTTTACCTACATTTTTAAAACGCATATGTTTGAGAGAGATAAGTTTCAATACCCTTATAAAGGATATTTTGTTCTTCTCCCCGACCTTTTTAAGACGCATATATTAGAGAGGGATAAGTTTCAATACCCTTATATAAAGGATATTTTGTTCTTCTCCCCAACAGATGTGGGATCTCACAATCCGTCCCCTTCAGGGTCCACCATCCTTGCTGACACTCGTTTCTTTCTCAAATCAATGTGGGACCCCAATCCACTCCCCTTTGGGGCTTAGCCTCTTCGCTGGCACATCGCCCCGTGTCTGGCTCTGATACCATTTGTGTAACAGCCCAAGCCCACTAGTATTAGATATTATCATCCCCGAACTTTCTCTCTAGATTCCATTCAAAGTTTTAAAATGCTTATATTAGAGAAAAGTTTCTACACCCTTATAAAATGTTTCGTTCTTATCCCAAATTGATGCGGGATCTCACATATATCAATTAAAAAATTTAAAATAATGGAATGATATTTAATAATAATGAAAACATATTTGCATTGAATGTGGAAATGAAGGTGCGATGGGTGCGGTTAGCGGGGCATCTCCAATGATATCGGCGCTGGGTGTAAACACAAGCAAATCCACATCTCCAGTTTATTTGTTGCCTAGATTTGGTGAGCATCACCCAACCAGAGATTGGAGACAGCCCATTCAAATTCCTTCAAGCCTTTGGCTACTCCATGTTGGCAATGCCTTTGAGCTTACCAACCCACAAGGCAACTTGGACTTCCAAATCCATGCCTCTGCTTGCTCTTATCAATGGTTCAATTTCGACAAACTATTCGGTACGACCTATTTTCCTCAATACTTCTCGTCCCATTTCATAACCATTTTGTTTTAAAAGGTAATCACTCTTATCTATTCCCGCAAGGTTATAATTGGCAGACGGAAAAACTCGACCCGTCAATTATGAACCTCAATGGAACCAAAAGTGAGTTGTTGCCTCGCCTTGTTAAGGTAAATGTTATTAAATTTGTTAGGAATCACCACTCTCCATAATGGTATTTTGACATTATCCATTTTGAACCCATGAGCATTGGAGGTTGTTAACCTATCATTATCTACCTTCTATTATGAACCTATCATTATCGTTATCATTCCTTATTTATAAACCCATGATCATTTCCTAAATTAGCCAAGGTGGGACTCCTTCCCAATAATCCTCAACGATTCTCTCATCGAACATACACCATAGAGTCTCCCCTAAGGCCTATAGAGCCTTCGTTCTCAGCGATAAACCCTACATTCTCAGGGATGAAAAACAATTACTTATATGCTGCAACTTCCTCCGGTTATCGGGAATCAATTAACCCCGCGTTCCGTTGATACGATTGTTATAAATCTTGAACACGTTCTTTAGTAACACCCGCCGAACATGGTCTGCGGGTAATCGGAGATTCGTCGGAGAGCCTGTTTTTGTTCCTAAAGGAGATGGAGAGGATGATGGATATGTTCTTGTGGTTGAGGTTAGCAAACTCATAAAACATCTTTTACTTTTTTTTTTTTATTAAACATTAAGAAAGTGAAGTAAATGATTTGAGTATGTAAATGCAGTACGCAGTATCAATACAAAGATGTTATCTTATGATTTTGGAGGCTAAAAAAATTGGAGAAGGCGATGCAGTTGTAGCAAGGCTTGAAGTCCCAAAGCATCTCAATTTTCCTCTTGGCTTCCATGGTTTCTGGGCTGCTAATACATAA

mRNA sequence

ATGCAGGCCAATCAACTCACTCCATCTCTCCGACCACAATTTCCGTCGCCGATCAAACTTCCTCCGGCGACCAGTATCCGATTTCCGCCTTCTCTAATTCCCAAAGCCATATCCATTCCCTCCCTGAACACTGGCCAGATATCGATCAATTCTCCTCCGTCGATCCCCACCGTTGATGATTCCATCGCAGCCTTCTGGGACTACCAGTTCCTCTTCGTTTCACAGCGGACCGAGACGGCGGACCCCACCGTCCTCCACCTGGTTGAAGGTGCCATCCCCGAAAATTTCCCTTCCGGCACCTACTACCTCGCCGGACCAGGCCTGTTCTCTGACGACCATGGCTCTACAGTACACCCCCTGGACGGCCACGGGTACCTCAGGGCATTCATTTTCGAGAACAAAAAGGAGGTCAGCTTCATGGCGAAGTACGTGAGAACGGAGGCGAAGATGGAGGAGCACGATCCTGCGACGGACACGTGGCGGTTCACGCATAGAGGGCCGTTCTCAGTGTTGAAGGGAGGGAGGAAGTTGGGGAATACGAAGGTTATGAAAAATGTGGCGAATACGAGTGTGCTGCAGTGGGGTGGCCACCTTTTGTGCCTATGGGAGGGCGGAGATCCGTACGAGATTCAGCCGGAGACTCTGGATACTGTCGGGAAGTTTTCAGGTGTTGACGGTGGCTGTGATCGTGATTCGCCATCCCAGGGCGGACGTCACGGTGCTGGGTTTTGGCGTTTTGCTGCGGAGCTTTTGAAGCCTGTTCTCTACGGAGTGTTCAAGATGCCACCAAGGAGGCTGTTGTCGCATTACAAGGTGGACGCTCAAAGGAATCGCCTCCTCTTAAACTCATGCAATGCTGAAGATATGCTTCTACCTACCAGTCATTTCACATTTTATGAATTTGACAGTAATTTCAAGTTATTAGAGAAGAAAGAATTTGTGATCGATGATCACCTGATGATTCATGATTGGGCTTTTACCGATACTCATTACATCCTCTTCGCTAACAGAATAAAGCTTGATCTCTTCGGTATATTGTTCTTTAACACCCGCCGAACATGGTCTGCGGGTAATCGGAGATTCGTCGGAGAGCCTGTTTTTGTTCCTAAAGGAGATGGAGAGGATGATGGATATGTTCTTGTGGTTGAGTACGCAGTATCAATACAAAGATGTTATCTTATGATTTTGGAGGCTAAAAAAATTGGAGAAGGCGATGCAGTTGTAGCAAGGCTTGAAGTCCCAAAGCATCTCAATTTTCCTCTTGGCTTCCATGGTTTCTGGGCTGCTAATACATAA

Coding sequence (CDS)

ATGCAGGCCAATCAACTCACTCCATCTCTCCGACCACAATTTCCGTCGCCGATCAAACTTCCTCCGGCGACCAGTATCCGATTTCCGCCTTCTCTAATTCCCAAAGCCATATCCATTCCCTCCCTGAACACTGGCCAGATATCGATCAATTCTCCTCCGTCGATCCCCACCGTTGATGATTCCATCGCAGCCTTCTGGGACTACCAGTTCCTCTTCGTTTCACAGCGGACCGAGACGGCGGACCCCACCGTCCTCCACCTGGTTGAAGGTGCCATCCCCGAAAATTTCCCTTCCGGCACCTACTACCTCGCCGGACCAGGCCTGTTCTCTGACGACCATGGCTCTACAGTACACCCCCTGGACGGCCACGGGTACCTCAGGGCATTCATTTTCGAGAACAAAAAGGAGGTCAGCTTCATGGCGAAGTACGTGAGAACGGAGGCGAAGATGGAGGAGCACGATCCTGCGACGGACACGTGGCGGTTCACGCATAGAGGGCCGTTCTCAGTGTTGAAGGGAGGGAGGAAGTTGGGGAATACGAAGGTTATGAAAAATGTGGCGAATACGAGTGTGCTGCAGTGGGGTGGCCACCTTTTGTGCCTATGGGAGGGCGGAGATCCGTACGAGATTCAGCCGGAGACTCTGGATACTGTCGGGAAGTTTTCAGGTGTTGACGGTGGCTGTGATCGTGATTCGCCATCCCAGGGCGGACGTCACGGTGCTGGGTTTTGGCGTTTTGCTGCGGAGCTTTTGAAGCCTGTTCTCTACGGAGTGTTCAAGATGCCACCAAGGAGGCTGTTGTCGCATTACAAGGTGGACGCTCAAAGGAATCGCCTCCTCTTAAACTCATGCAATGCTGAAGATATGCTTCTACCTACCAGTCATTTCACATTTTATGAATTTGACAGTAATTTCAAGTTATTAGAGAAGAAAGAATTTGTGATCGATGATCACCTGATGATTCATGATTGGGCTTTTACCGATACTCATTACATCCTCTTCGCTAACAGAATAAAGCTTGATCTCTTCGGTATATTGTTCTTTAACACCCGCCGAACATGGTCTGCGGGTAATCGGAGATTCGTCGGAGAGCCTGTTTTTGTTCCTAAAGGAGATGGAGAGGATGATGGATATGTTCTTGTGGTTGAGTACGCAGTATCAATACAAAGATGTTATCTTATGATTTTGGAGGCTAAAAAAATTGGAGAAGGCGATGCAGTTGTAGCAAGGCTTGAAGTCCCAAAGCATCTCAATTTTCCTCTTGGCTTCCATGGTTTCTGGGCTGCTAATACATAA

Protein sequence

MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDDSIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHGAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGILFFNTRRTWSAGNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNFPLGFHGFWAANT

Homology

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BLAST of Carg28253 vs. NCBI nr
Match: KAG7013829.1 (Carotenoid cleavage dioxygenase 7, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 899.8 bits (2324), Expect = 9.0e-258
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD
Sbjct: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG
Sbjct: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGILFFNTRRTWSAGNRR 360
           FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGILFFNTRRTWSAGNRR
Sbjct: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGILFFNTRRTWSAGNRR 360

Query: 361 FVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNFP 420
           FVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNFP
Sbjct: 361 FVGEPVFVPKGDGEDDGYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVARLEVPKHLNFP 420

Query: 421 LGFHGFWAANT 432
           LGFHGFWAANT
Sbjct: 421 LGFHGFWAANT 431

[Close]

BLAST of Carg28253 vs. NCBI nr
Match: XP_022929872.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita moschata] >KAG6575282.1 Carotenoid cleavage dioxygenase 7, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 813.5 bits (2100), Expect = 8.5e-232
Identity = 427/615 (69.43%), Postives = 429/615 (69.76%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD
Sbjct: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG
Sbjct: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL-------------- 360
           FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFG +              
Sbjct: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALG 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHAS 420

Query: 421 -----FFN---------------------------------------------------- 432
                +FN                                                    
Sbjct: 421 ACSYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPRLVKVSISLSKNGECEKCSVEP 480

[Close]

BLAST of Carg28253 vs. NCBI nr
Match: XP_023548386.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.0 bits (2052), Expect = 3.1e-226
Identity = 417/614 (67.92%), Postives = 421/614 (68.57%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQAN+LTPSLRPQFPSPIKLPPAT IRFPPSLIPKAISIPS NTGQISINSPPSIPTV+D
Sbjct: 1   MQANRLTPSLRPQFPSPIKLPPATGIRFPPSLIPKAISIPSPNTGQISINSPPSIPTVED 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVL LVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGG LLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGG HG
Sbjct: 181 KVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGLHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL-------------- 360
           FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFG +              
Sbjct: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALG 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHAS 420

Query: 421 ------------------------------------------------------------ 431
                                                                       
Sbjct: 421 ACSYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPHLVKVSISLSKNGECEKCSVEP 480

[Close]

BLAST of Carg28253 vs. NCBI nr
Match: XP_022973887.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita maxima])

HSP 1 Score: 787.3 bits (2032), Expect = 6.5e-224
Identity = 415/614 (67.59%), Postives = 418/614 (68.08%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQANQLTPSLRPQFPSPIKLPPA  IRFP SLIPKAISIPS NT QISINSP SIPTVDD
Sbjct: 1   MQANQLTPSLRPQFPSPIKLPPAAGIRFPLSLIPKAISIPSPNTSQISINSPLSIPTVDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVL LVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMA+YVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMARYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGG LLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG
Sbjct: 181 KVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGI--------------- 360
           FDSNF+LLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFG                
Sbjct: 301 FDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISSLG 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTDPQGNLDFQIHAS 420

Query: 421 ------------------------------------------------------------ 431
                                                                       
Sbjct: 421 ACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKSELLPRLVKVSFRLSKNGECEKCSVEP 480

[Close]

BLAST of Carg28253 vs. NCBI nr
Match: XP_038876141.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Benincasa hispida])

HSP 1 Score: 680.6 bits (1755), Expect = 8.6e-192
Identity = 363/615 (59.02%), Postives = 388/615 (63.09%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           M+ N LTPSL  QFPSPIKLPP T IRFP SLIPKAISIP+ NT +ISIN        DD
Sbjct: 1   MKTNSLTPSLPLQFPSPIKLPPVTVIRFPSSLIPKAISIPTPNTTRISIND-------DD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTET DPTVL LV+GAIP +FPSGTYYLAGPG+FSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETTDPTVLRLVDGAIPPDFPSGTYYLAGPGMFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFE-NKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGN 180
           DGHGYLRAF+FE  KKEV FMAKYV+TEAKMEEHD  TDTWRFTHRGPFSVLKGG+KLGN
Sbjct: 121 DGHGYLRAFVFERKKKEVVFMAKYVKTEAKMEEHDLVTDTWRFTHRGPFSVLKGGKKLGN 180

Query: 181 TKVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRH 240
           TKVMKNVANTSVL+WGG LLCLWEGGDPYEI+PE LDTVGKFS V GGCD DSPS GG +
Sbjct: 181 TKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRPENLDTVGKFSAVHGGCDCDSPSHGGPY 240

Query: 241 GAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFY 300
           GAGFWRFAAELLKPVLYGVFKMPP+RLLSHYK+DAQRNRLL+ SCNAEDMLLPTSHFTFY
Sbjct: 241 GAGFWRFAAELLKPVLYGVFKMPPKRLLSHYKLDAQRNRLLIMSCNAEDMLLPTSHFTFY 300

Query: 301 EFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGI-------------- 360
           EFDSNFKLL+KK+ VIDDHLMIHDWAFTDTHYILFANRIKLD+ G               
Sbjct: 301 EFDSNFKLLQKKDLVIDDHLMIHDWAFTDTHYILFANRIKLDVIGAMGAVSGISPMISAL 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 SVNTSKPTSPIYLIPRFGEDSNRNLRKAVVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHA 420

Query: 421 ------------------------------------------------------------ 431
                                                                       
Sbjct: 421 SACSYQWFNFKKLFGYNWQTGKLDPSIMNLNESKSELLPRLVKISISLNKNEKCEKCSVE 480

[Close]

BLAST of Carg28253 vs. ExPASy Swiss-Prot
Match: Q7XJM2 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD7 PE=1 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 4.4e-138
Identity = 282/619 (45.56%), Postives = 336/619 (54.28%), Query Frame = 0


Query: 12  PQFPSPIKLPPATSIRFPPSLIPK----AISIPSLNTGQISINSPPSIPTVDDSI-AAFW 71
           P+F  P+K PP    + PP L P     +ISIP    G+           +D+S  +AF 
Sbjct: 7   PKFLPPLKSPPIHHHQTPPPLAPPRAAISISIPDTGLGRTG-------TILDESTSSAFR 66

Query: 72  DYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYL 131
           DYQ LFVSQR+ET +P V+  +EG+IP NFPSGTYYLAGPGLF+DDHGSTVHPLDGHGYL
Sbjct: 67  DYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGTYYLAGPGLFTDDHGSTVHPLDGHGYL 126

Query: 132 RAF-IFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKN 191
           RAF I  NK++ +F AKYV+TEAK EEHDP TDTWRFTHRGPFSVLKGG++ GNTKVMKN
Sbjct: 127 RAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDTWRFTHRGPFSVLKGGKRFGNTKVMKN 186

Query: 192 VANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGG---CDRDSPSQGGRHGAG 251
           VANTSVL+W G LLCLWEGG+PYEI+  +LDTVG+F+  + G   CD D  S     G  
Sbjct: 187 VANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVGRFNVENNGCESCDDDDSSDRDLSGHD 246

Query: 252 FWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFD 311
            W  AA+LLKP+L GVFKMPP+R LSHYKVD +R RLL  +CNAEDMLLP S+FTF E+D
Sbjct: 247 IWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRRKRLLTVTCNAEDMLLPRSNFTFCEYD 306

Query: 312 SNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLD--------------------- 371
           S FKL++ KEF IDDH+MIHDWAFTDTHYILFANR+KL+                     
Sbjct: 307 SEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFANRVKLNPIGSIAAMCGMSPMVSALSLN 366

Query: 372 ------------------------------------------------------------ 431
                                                                       
Sbjct: 367 PSNESSPIYILPRFSDKYSRGGRDWRVPVEVSSQLWLIHSGNAYETREDNGDLKIQIQAS 426

[Close]

BLAST of Carg28253 vs. ExPASy Swiss-Prot
Match: Q7XU29 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD7 PE=1 SV=2)

HSP 1 Score: 418.7 bits (1075), Expect = 8.0e-116
Identity = 238/561 (42.42%), Postives = 284/561 (50.62%), Query Frame = 0


Query: 59  DDSIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVH 118
           D   AAFWDY  LF SQR E  D   L + EGAIP +FP+GTYYLAGPG+FSDDHGSTVH
Sbjct: 47  DTLSAAFWDYNLLFRSQRDECLDSIPLRVTEGAIPPDFPAGTYYLAGPGIFSDDHGSTVH 106

Query: 119 PLDGHGYLRAFIFE-NKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKL 178
           PLDGHGYLR+F F    + + + A++V T AK EE      +WRFTHRGPFSVL+GG+K+
Sbjct: 107 PLDGHGYLRSFRFRPGDRTIHYSARFVETAAKREESRDGA-SWRFTHRGPFSVLQGGKKV 166

Query: 179 GNTKVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGG 238
           GN KVMKNVANTSVL+WGG LLCLWEGG PYE+ P TL+TVG F  +      D+ +   
Sbjct: 167 GNVKVMKNVANTSVLRWGGRLLCLWEGGQPYEVDPRTLETVGPFDLLGLAAADDNKATNA 226

Query: 239 RHGAGFW------RFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLL 298
                 W        AA LL+PVL GVF MP +RLL+HYK+D +R RLL+ +CNAEDMLL
Sbjct: 227 SAARRPWLQEAGLDAAARLLRPVLSGVFDMPGKRLLAHYKIDPRRGRLLMVACNAEDMLL 286

Query: 299 PTSHFTFYEFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL----- 358
           P SHFTFYEFD++F L++K+EFV+ DHLMIHDWAFTDTHYIL  NRIKLD+ G L     
Sbjct: 287 PRSHFTFYEFDAHFDLVQKREFVVPDHLMIHDWAFTDTHYILLGNRIKLDIPGSLLALTG 346

Query: 359 ------------------------------------------------------------ 418
                                                                       
Sbjct: 347 THPMIAALAVDPRRQSTPVYLLPRSPETEAGGRDWSVPIEAPSQMWSVHVGNAFEEANRR 406

Query: 419 -----------------------------------FFN---------------------- 428
                                              F N                      
Sbjct: 407 GGLDVRLHMSSCSYQWFHFHRMFGYNWHHKKLDPSFMNAAKGKEWLPRLVQVAIELDRTG 466

[Close]

BLAST of Carg28253 vs. ExPASy Swiss-Prot
Match: P74334 (Apocarotenoid-15,15'-oxygenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1541 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.3e-19
Identity = 77/264 (29.17%), Postives = 105/264 (39.77%), Query Frame = 0


Query: 88  VEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFENKKEVSFMAKYVRTE 147
           VEG+IP +   GT Y  GPGL         HP DG G + AF F     V F +K+VRT+
Sbjct: 38  VEGSIPPDL-QGTLYRNGPGLLEIGDRPLKHPFDGDGMVTAFKFPGDGRVHFQSKFVRTQ 97

Query: 148 AKMEEHDPATDTWRFTHRGPFSVLKGGRKLGN--TKVMKNVANTSVLQWGGHLLCLWEGG 207
             +EE        +  +RG F     G  L       +KN+ANT++  WG  LL LWEGG
Sbjct: 98  GYVEEQKAG----KMIYRGVFGSQPAGGWLKTIFDLRLKNIANTNITYWGDRLLALWEGG 157

Query: 208 DPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHGAGFWRFAAELLKPVLYGVFKMPPRR 267
            P+ ++P  L T+G                GG    G                     + 
Sbjct: 158 QPHRLEPSNLATIG------------LDDLGGILAEG---------------------QP 217

Query: 268 LLSHYKVDAQRNRLLLNSCNAEDMLLP--TSHFTFYEFDSNFKLLEKKEFVIDDHLMIHD 327
           L +H ++D          C     +    +S  T  E D   KLL +K         IHD
Sbjct: 218 LSAHPRIDPASTFDGGQPCYVTFSIKSSLSSTLTLLELDPQGKLLRQKTETFPGFAFIHD 263

Query: 328 WAFTDTHYILFANRIKLDLFGILF 348
           +A T  + I   N + L+    LF
Sbjct: 278 FAITPHYAIFLQNNVTLNGLPYLF 263

[Close]

BLAST of Carg28253 vs. ExPASy TrEMBL
Match: A0A6J1ENV6 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436349 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.1e-232
Identity = 427/615 (69.43%), Postives = 429/615 (69.76%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD
Sbjct: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG
Sbjct: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL-------------- 360
           FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFG +              
Sbjct: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISALG 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTNPQGNLDFQIHAS 420

Query: 421 -----FFN---------------------------------------------------- 432
                +FN                                                    
Sbjct: 421 ACSYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKSELLPRLVKVSISLSKNGECEKCSVEP 480

[Close]

BLAST of Carg28253 vs. ExPASy TrEMBL
Match: A0A6J1I8R3 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472478 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 415/614 (67.59%), Postives = 418/614 (68.08%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           MQANQLTPSLRPQFPSPIKLPPA  IRFP SLIPKAISIPS NT QISINSP SIPTVDD
Sbjct: 1   MQANQLTPSLRPQFPSPIKLPPAAGIRFPLSLIPKAISIPSPNTSQISINSPLSIPTVDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLFVSQRTETADPTVL LVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAFIFENKKEVSFMA+YVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT
Sbjct: 121 DGHGYLRAFIFENKKEVSFMARYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVLQWGG LLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG
Sbjct: 181 KVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
           AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE
Sbjct: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGI--------------- 360
           FDSNF+LLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFG                
Sbjct: 301 FDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGAMGAVSGASPMISSLG 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTSKSTSPVYLLPRFGEHHPTRDWRQPIQIPSSLWLLHVGNAFELTDPQGNLDFQIHAS 420

Query: 421 ------------------------------------------------------------ 431
                                                                       
Sbjct: 421 ACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKSELLPRLVKVSFRLSKNGECEKCSVEP 480

[Close]

BLAST of Carg28253 vs. ExPASy TrEMBL
Match: T1UMH0 (Carotenoid cleavage dioxygenase OS=Cucumis melo OX=3656 GN=CCD7 PE=2 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 6.9e-187
Identity = 355/616 (57.63%), Postives = 383/616 (62.18%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKLPPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVDD 60
           M+AN LTPSL  QFPSP+KLPP T IRFP SL PKAISI + NT +ISIN        DD
Sbjct: 1   MKANSLTPSLPLQFPSPVKLPPPTGIRFPSSLTPKAISISTPNTNRISINK-----NDDD 60

Query: 61  SIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPL 120
           SIAAFWDYQFLF+SQRTET +P VL LV+GAIP +FPSGTYYL GPG+FSDDHGSTVHPL
Sbjct: 61  SIAAFWDYQFLFISQRTETENPAVLRLVDGAIPPDFPSGTYYLTGPGMFSDDHGSTVHPL 120

Query: 121 DGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNT 180
           DGHGYLRAF FE K EV+FMAKYV+TEAKMEEHDP TD WRFTHRGPFSVLKGG+KLGNT
Sbjct: 121 DGHGYLRAFAFE-KDEVAFMAKYVKTEAKMEEHDPETDRWRFTHRGPFSVLKGGKKLGNT 180

Query: 181 KVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRHG 240
           KVMKNVANTSVL+WGG LLCLWEGGDPYEI+PE LDTVGKFS  DGG D DSPS+GG + 
Sbjct: 181 KVMKNVANTSVLRWGGRLLCLWEGGDPYEIRPEDLDTVGKFSAFDGGGDHDSPSRGGTYD 240

Query: 241 AGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYE 300
            GFWRFAAELLKPVLYGVFKMPP+RLLSHYKVDAQRNRLL+ SCNAEDMLLPTSHFTFYE
Sbjct: 241 GGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLLIMSCNAEDMLLPTSHFTFYE 300

Query: 301 FDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL-------------- 360
           FDSNFKLL+KK+ VIDDHLMIHDWAFTD HYILFANRIKLD+ G +              
Sbjct: 301 FDSNFKLLQKKDLVIDDHLMIHDWAFTDNHYILFANRIKLDVIGAMGAITGVSPMISALS 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 VNTNKSTSPIYLIPRFGEDSNKDCKETIVEVPSRLWVLHVGNAFEIIHEEDGTLDFQIHA 420

Query: 421 ------FFN--------------------------------------------------- 432
                 +FN                                                   
Sbjct: 421 SSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPHLVKVCISLSKNGKCEKCSVE 480

[Close]

BLAST of Carg28253 vs. ExPASy TrEMBL
Match: A0A0A0KAN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106700 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.3e-182
Identity = 349/617 (56.56%), Postives = 377/617 (61.10%), Query Frame = 0


Query: 1   MQANQLTPSLRPQFPSPIKL-PPATSIRFPPSLIPKAISIPSLNTGQISINSPPSIPTVD 60
           M+AN LT SL  QFPSPIKL PP+T IRFP SL PKAISI + NT +ISIN        D
Sbjct: 1   MKANSLTSSLPLQFPSPIKLPPPSTGIRFPSSLTPKAISISTPNTHRISINKNDD---DD 60

Query: 61  DSIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHP 120
           DSIAAFWDYQFLF+SQRTET +P VL LV+GAIP +FPSGTYYL GPG+FSDDHGSTVHP
Sbjct: 61  DSIAAFWDYQFLFISQRTETENPAVLRLVDGAIPSDFPSGTYYLTGPGMFSDDHGSTVHP 120

Query: 121 LDGHGYLRAFIFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGN 180
           LDGHGYLRAF+FE   EV+FMAKYV+TEAKMEEHDP TD WRFTHRGPFSVLKGG+KLGN
Sbjct: 121 LDGHGYLRAFVFEKDHEVTFMAKYVKTEAKMEEHDPETDRWRFTHRGPFSVLKGGKKLGN 180

Query: 181 TKVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGGCDRDSPSQGGRH 240
           TKVMKNVANTSVL+WGG LLCLWEGGDPYEI+ E LDTVGKF    GG D DSPS+GG +
Sbjct: 181 TKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRAEDLDTVGKFCAFHGGDDHDSPSRGGGY 240

Query: 241 GAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFY 300
             GFWRFAAELLKPVLYGVFKMPP+RLLSHYKVDAQRNRLL+ SCNAEDMLLPTSHFTFY
Sbjct: 241 DGGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLLVMSCNAEDMLLPTSHFTFY 300

Query: 301 EFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL------------- 360
           EFDSNFKLL+KK+ VIDDHLMIHDWAFTD HYILFANRIKLD+ G +             
Sbjct: 301 EFDSNFKLLQKKDMVIDDHLMIHDWAFTDNHYILFANRIKLDVIGAMSAISGISPMISAL 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 SVNTNKSTSPIYLIPRFGEDSKKDDWKETIVEVPSRLWLLHVGNAFETIHEDGTLDFEIH 420

Query: 421 ------------------------------------------------------------ 432
                                                                       
Sbjct: 421 ASSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPHLVKVSISLSKNGKCEKCSV 480

[Close]

BLAST of Carg28253 vs. ExPASy TrEMBL
Match: A0A6J1DS65 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023796 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.4e-163
Identity = 322/611 (52.70%), Postives = 364/611 (59.57%), Query Frame = 0


Query: 13  QFPSPIKLPPATSIRFPPS-LIPKAISIPSLNTGQISINSPPSIPTVDDSIAAFWDYQFL 72
           QFPSP+ L  AT I FP S +IP+A +  S++  + +  S P     DDS+AAFWDYQFL
Sbjct: 15  QFPSPVTLLQATRILFPASPIIPRAAT--SISGRRHNQTSLPPAEGDDDSVAAFWDYQFL 74

Query: 73  FVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIF 132
           FVSQR+ETA+P VL  V+GA+P +FP GTYYLAGPGLF+DDHGSTVHPLDGHGYLRAF F
Sbjct: 75  FVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGSTVHPLDGHGYLRAFGF 134

Query: 133 ENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSV 192
           EN KEV F AKYVRTEA+MEEH+ ATDTWRFTHRGPFSVLKGG++LGNTKVMKNVANTSV
Sbjct: 135 EN-KEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGGKRLGNTKVMKNVANTSV 194

Query: 193 LQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVD--------GGCDRDSPSQGGRHGAGF 252
           L+W G LLCLWEGGDPYEIQP +LDT+GKFS VD        GGC R       RHGA  
Sbjct: 195 LRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGGGCGRGG---SPRHGADL 254

Query: 253 WRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFDS 312
           WRF A LLKP+L+GVFKMPP+RLLSHYK+DAQRNRLL+ SC AEDMLLPTS+FTFYEFDS
Sbjct: 255 WRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCKAEDMLLPTSNFTFYEFDS 314

Query: 313 NFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLDLFGIL----------------- 372
           NFKLL+K++FVIDDHLMIHDWAFTDTHY++FANRIKLD+ G +                 
Sbjct: 315 NFKLLQKQDFVIDDHLMIHDWAFTDTHYVIFANRIKLDVIGAMGAVSGISPMISALSVNG 374

Query: 373 ------------------------------------------------------------ 432
                                                                       
Sbjct: 375 SKATCPVYLLPRFAEDGNRDWREAVEVPSRLWLLHVGNAFEVRDDDGNLDIQIHASACSY 434

[Close]

BLAST of Carg28253 vs. TAIR 10
Match: AT2G44990.1 (carotenoid cleavage dioxygenase 7 )

HSP 1 Score: 459.1 bits (1180), Expect = 3.8e-129
Identity = 272/619 (43.94%), Postives = 328/619 (52.99%), Query Frame = 0


Query: 12  PQFPSPIKLPPATSIRFPPSLIPK----AISIPSLNTGQISINSPPSIPTVDDSI-AAFW 71
           P+F  P+K PP    + PP L P     +ISIP    G+           +D+S  +AF 
Sbjct: 7   PKFLPPLKSPPIHHHQTPPPLAPPRAAISISIPDTGLGRTG-------TILDESTSSAFR 66

Query: 72  DYQFLFVSQRTETADPTVLHLVEGAIPENFPSGTYYLAGPGLFSDDHGSTVHPLDGHGYL 131
           DYQ LFVSQR+ET +P V+  +EG+IP NFPSGTYYLAGPGLF+DDHGSTVHPLDGHGYL
Sbjct: 67  DYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGTYYLAGPGLFTDDHGSTVHPLDGHGYL 126

Query: 132 RAF-IFENKKEVSFMAKYVRTEAKMEEHDPATDTWRFTHRGPFSVLKGGRKLGNTKVMKN 191
           RAF I  NK++ +F AKYV+TEAK EEHDP TDTWRFTHRGPFSVLKGG++ GNTKVMKN
Sbjct: 127 RAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDTWRFTHRGPFSVLKGGKRFGNTKVMKN 186

Query: 192 VANTSVLQWGGHLLCLWEGGDPYEIQPETLDTVGKFSGVDGG---CDRDSPSQGGRHGAG 251
           VANTSVL+W G LLCLWEGG+PYEI+  +LDTVG+F+  + G   CD D  S     G  
Sbjct: 187 VANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVGRFNVENNGCESCDDDDSSDRDLSGHD 246

Query: 252 FWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQRNRLLLNSCNAEDMLLPTSHFTFYEFD 311
            W  AA+LLKP+L GVFKMPP+R LSHYKVD +R RLL  +CNAEDMLLP S+FTF E+D
Sbjct: 247 IWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRRKRLLTVTCNAEDMLLPRSNFTFCEYD 306

Query: 312 SNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFANRIKLD--------------------- 371
           S FKL++ KEF IDDH+MIHDWAFTDTHYILFANR+KL+                     
Sbjct: 307 SEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFANRVKLNPIGSIAAMCGMSPMVSALSLN 366

Query: 372 ------------------------------------------------------------ 428
                                                                       
Sbjct: 367 PSNESSPIYILPRFSDKYSRGGRDWRVPVEVSSQLWLIHSGNAYETREDNGDLKIQIQAS 426

[Close]

BLAST of Carg28253 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 43.5 bits (101), Expect = 5.0e-04
Identity = 24/75 (32.00%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 352 RTWSAGNRRFVGEPVFVPKGDGEDD-GYVLVVEYAVSIQRCYLMILEAKKIGEGDAVVAR 411
           R +  G  ++ GEP+F+P GDGE+D GY++V  +     +  L ++ A  +     + A 
Sbjct: 575 RKYIYGEGKYGGEPLFLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNM----KLEAT 634

Query: 412 LEVPKHLNFPLGFHG 426
           + +P  +  P GFHG
Sbjct: 635 VTLPSRV--PYGFHG 643

The following BLAST results are available for this feature:

BLAST of Carg28253 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013829.1	9.0e-258	100.00	Carotenoid cleavage dioxygenase 7, chloroplastic, partial [Cucurbita argyrosperm...	[more]
XP_022929872.1	8.5e-232	69.43	carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita moschata] >KAG657528...	[more]
XP_023548386.1	3.1e-226	67.92	carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022973887.1	6.5e-224	67.59	carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita maxima]	[more]
XP_038876141.1	8.6e-192	59.02	carotenoid cleavage dioxygenase 7, chloroplastic [Benincasa hispida]	[more]

BLAST of Carg28253 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XJM2	4.4e-138	45.56	Carotenoid cleavage dioxygenase 7, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q7XU29	8.0e-116	42.42	Carotenoid cleavage dioxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica...	[more]
P74334	5.3e-19	29.17	Apocarotenoid-15,15'-oxygenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O...	[more]

BLAST of Carg28253 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ENV6	4.1e-232	69.43	carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1I8R3	3.2e-224	67.59	carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita maxima OX=3661 GN=...	[more]
T1UMH0	6.9e-187	57.63	Carotenoid cleavage dioxygenase OS=Cucumis melo OX=3656 GN=CCD7 PE=2 SV=1	[more]
A0A0A0KAN6	1.3e-182	56.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106700 PE=3 SV=1	[more]
A0A6J1DS65	2.4e-163	52.70	carotenoid cleavage dioxygenase 7, chloroplastic OS=Momordica charantia OX=3673 ...	[more]

BLAST of Carg28253 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44990.1	3.8e-129	43.94	carotenoid cleavage dioxygenase 7	[more]
AT1G78390.1	5.0e-04	32.00	nine-cis-epoxycarotenoid dioxygenase 9	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 353..428 e-value: 5.3E-19 score: 68.7 coord: 73..342 e-value: 2.8E-45 score: 155.3
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 356..429 coord: 53..345
None	No IPR available	PANTHER	PTHR10543:SF37	CAROTENOID CLEAVAGE DIOXYGENASE 7, CHLOROPLASTIC	coord: 356..429 coord: 53..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28253-RA	Carg28253-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
biological_process	GO:0009733	response to auxin
biological_process	GO:0010223	secondary shoot formation
biological_process	GO:1901601	strigolactone biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen