Carg28224 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg28224 vs. NCBI nr
Match: XP_022932903.1 (probable N-acetyltransferase HLS1 isoform X1 [Cucurbita moschata] >KAG7011045.1 putative N-acetyltransferase HLS1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 861.7 bits (2225), Expect = 2.6e-246
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF
Sbjct: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 419

BLAST of Carg28224 vs. NCBI nr
Match: XP_022932904.1 (probable N-acetyltransferase HLS1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 855.1 bits (2208), Expect = 2.5e-244
Identity = 418/419 (99.76%), Postives = 418/419 (99.76%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDH RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF
Sbjct: 121 YILGLRVSPDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Carg28224 vs. NCBI nr
Match: XP_023512928.1 (probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo] >XP_023522343.1 probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.4e-242
Identity = 415/420 (98.81%), Postives = 417/420 (99.29%), Query Frame = 0

Query: 1   MADH-KKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPI 60
           MADH KKKKKKRKNNIQILIREFDPIKD+ +VEDVERRCEVGSTKNKNFSLFTDLLGDPI
Sbjct: 1   MADHKKKKKKKRKNNIQILIREFDPIKDRTAVEDVERRCEVGSTKNKNFSLFTDLLGDPI 60

Query: 61  CRVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKL 120
           CRVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKL
Sbjct: 61  CRVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKL 120

Query: 121 AYILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSK 180
           AYILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSK
Sbjct: 121 AYILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSK 180

Query: 181 FRTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSL 240
           FRTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSL
Sbjct: 181 FRTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSL 240

Query: 241 GTFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLS 300
           GTFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLS
Sbjct: 241 GTFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLS 300

Query: 301 RVIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVV 360
           RVIDRGLPWLRLPSVPEVF PFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVV
Sbjct: 301 RVIDRGLPWLRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVV 360

Query: 361 ATEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           ATEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 ATEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420

BLAST of Carg28224 vs. NCBI nr
Match: KAG6571244.1 (putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846.7 bits (2186), Expect = 8.8e-242
Identity = 413/419 (98.57%), Postives = 416/419 (99.28%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MADH KKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MADH-KKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEI+AHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQ+APVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEISAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQIAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDHRRLGIGLNLVRRMEEWFREK+AEYSYIATEIDNVASIKLFTHKCGYSKF
Sbjct: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKRAEYSYIATEIDNVASIKLFTHKCGYSKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDR LPWLRLPSVPEVF PFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRVLPWLRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Carg28224 vs. NCBI nr
Match: XP_022986693.1 (probable N-acetyltransferase HLS1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 830.1 bits (2143), Expect = 8.5e-237
Identity = 405/419 (96.66%), Postives = 411/419 (98.09%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MAD +KKKKKRKNNIQILIREFDP KD+P+VEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MAD-QKKKKKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRN+GINASDLP LAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGY KF
Sbjct: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTIL+LELTVAETLYRCRFA AEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAK SR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVF PFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGE YT+NSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Carg28224 vs. ExPASy Swiss-Prot
Match: Q42381 (Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.5e-154
Identity = 265/406 (65.27%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 18  LIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAEIAA 77
           ++RE+DP +D   VEDVERRCEVG   +   SLFTDLLGDPICR+RHSP++LMLVAE+  
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVG--PSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGT 62

Query: 78  H-NEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVSPDHRRLGI 137
              EIVGMIRGCIKTVTC  K   N   + S    + P+YTKLAY+LGLRVSP HRR GI
Sbjct: 63  EKKEIVGMIRGCIKTVTCGQKLDLN---HKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 138 GLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNPVFAHRLR 197
           G  LV+ MEEWFR+  AEYSYIATE DN AS+ LFT KCGYS+FRTPSILVNPV+AHR+ 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 198 LSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGS---FTDG 257
           +S +VT+++LE   AETLYR RF+T EFFPRDID+VL+N+LSLGTF+AVPRGS      G
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 258 LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPWLRLPS 317
            W  S +FL   PESWAVLS WNCKD + LEVRG S ++R +AK +RV+D+ LP+L+LPS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 318 VPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEPLKSDI 377
           +P VF+PFG+ F+YG+GGEGP A K++K+LC HAHNLAK  GCGVVA EV+ ++PL+  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 378 PHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           PHWK LSC EDLWCIKRLG+ Y++  +GDWTKSPP  SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of Carg28224 vs. ExPASy Swiss-Prot
Match: O64815 (Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23060 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-152
Identity = 263/413 (63.68%), Postives = 323/413 (78.21%), Query Frame = 0

Query: 15  IQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAE 74
           + + +RE+DP KD  +VEDVERRCEVG       SLFTDLLGDPICRVRHSP++LMLVAE
Sbjct: 3   VLVEVREYDPSKDLATVEDVERRCEVGPA--GKLSLFTDLLGDPICRVRHSPSYLMLVAE 62

Query: 75  IA--AHNEIVGMIRGCIKTVTCCSKSTR---NAGINASDLPQLAPVYTKLAYILGLRVSP 134
           I      E+VGMIRGCIKTVTC   + R       + +D+    P+YTKLAYILGLRVSP
Sbjct: 63  IGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSP 122

Query: 135 DHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNP 194
            HRR GIG  LV+ ME+WF +  AEYSY ATE DN AS+ LFT KCGY++FRTPSILVNP
Sbjct: 123 THRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNP 182

Query: 195 VFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGS 254
           V+AHR+ +S +VT+++LE + AE LYR RF+T EFFPRDID+VL+N+LSLGTF+AVPRGS
Sbjct: 183 VYAHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGS 242

Query: 255 -FTDG--LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGL 314
            +  G   W  S +FL   P+SWAVLS WNCKD + LEVRG S ++R ++K +R++D+ L
Sbjct: 243 CYGSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTL 302

Query: 315 PWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSND 374
           P+L++PS+P VF+PFG+ F+YG+GGEGP A K++KALC HAHNLAKE GCGVVA EV+ +
Sbjct: 303 PFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGE 362

Query: 375 EPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           EPL+  IPHWK LSC EDLWCIKRLGE Y++ S+GDWTKSPP  SIFVDPREF
Sbjct: 363 EPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of Carg28224 vs. ExPASy TrEMBL
Match: A0A6J1F329 (probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439465 PE=4 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.3e-246
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF
Sbjct: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 419

BLAST of Carg28224 vs. ExPASy TrEMBL
Match: A0A6J1EYB0 (probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439465 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.2e-244
Identity = 418/419 (99.76%), Postives = 418/419 (99.76%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDH RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF
Sbjct: 121 YILGLRVSPDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Carg28224 vs. ExPASy TrEMBL
Match: A0A6J1JHA4 (probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484371 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.1e-237
Identity = 405/419 (96.66%), Postives = 411/419 (98.09%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MAD +KKKKKRKNNIQILIREFDP KD+P+VEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MAD-QKKKKKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRN+GINASDLP LAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGY KF
Sbjct: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTIL+LELTVAETLYRCRFA AEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAK SR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVF PFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGE YT+NSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Carg28224 vs. ExPASy TrEMBL
Match: A0A6J1JGS4 (probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484371 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 3.9e-235
Identity = 404/419 (96.42%), Postives = 410/419 (97.85%), Query Frame = 0

Query: 1   MADHKKKKKKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60
           MAD +KKKKKRKNNIQILIREFDP KD+P+VEDVERRCEVGSTKNKNFSLFTDLLGDPIC
Sbjct: 1   MAD-QKKKKKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPIC 60

Query: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLA 120
           RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRN+GINASDLP LAPVYTKLA
Sbjct: 61  RVRHSPAFLMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLA 120

Query: 121 YILGLRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKF 180
           YILGLRVSPDH RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGY KF
Sbjct: 121 YILGLRVSPDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKF 180

Query: 181 RTPSILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLG 240
           RTPSILVNPVFAHRLRLSDQVTIL+LELTVAETLYRCRFA AEFFPRDIDAVLSNRLSLG
Sbjct: 181 RTPSILVNPVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLG 240

Query: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSR 300
           TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAK SR
Sbjct: 241 TFLAVPRGSFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSR 300

Query: 301 VIDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360
           VIDRGLPWLRLPSVPEVF PFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA
Sbjct: 301 VIDRGLPWLRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVA 360

Query: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGE YT+NSLGDWTKSPPSFSIFVDPREF
Sbjct: 361 TEVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 417

BLAST of Carg28224 vs. ExPASy TrEMBL
Match: V4SFH0 (N-acetyltransferase domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10025754mg PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 1.1e-168
Identity = 294/405 (72.59%), Postives = 331/405 (81.73%), Query Frame = 0

Query: 17  ILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAEIA 76
           I++REFDP KD   VEDVERRCEVG   +    LFTDLLGDPICRVRHSPAFLMLVAE+ 
Sbjct: 6   IVVREFDPNKDCLGVEDVERRCEVG--PSGKLCLFTDLLGDPICRVRHSPAFLMLVAEVG 65

Query: 77  AHNEIVGMIRGCIKTVTCCSKSTRNAGINASDL--PQLAPVYTKLAYILGLRVSPDHRRL 136
             +EIVGMIRGCIKTVTC  + +RN     +D+  P+  PVYTKLAYILGLRVSP HRR+
Sbjct: 66  --DEIVGMIRGCIKTVTCGKRISRNTKYTTNDIEPPKPLPVYTKLAYILGLRVSPSHRRM 125

Query: 137 GIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNPVFAHR 196
           GIGL LV+RMEEWFRE   EYSYIATE DN AS+KLFT KCGYSKFRTPSILVNPVFAHR
Sbjct: 126 GIGLKLVKRMEEWFRESGVEYSYIATENDNYASVKLFTDKCGYSKFRTPSILVNPVFAHR 185

Query: 197 LRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGSFTDGL 256
           L +  QVTI+QL  + AE  YR +F+T EFFPRDID+VL+N+L+LGTFLAVPRG+++   
Sbjct: 186 LIVPKQVTIIQLNPSDAEAFYRRKFSTTEFFPRDIDSVLNNKLNLGTFLAVPRGTYSPDS 245

Query: 257 WVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPWLRLPSV 316
           W  SD F SC PESWA+LS WN KDV+ LEVRG S VKR +AK +RV+DR LPWLR+PSV
Sbjct: 246 WAGSDSFFSCPPESWAILSVWNSKDVFKLEVRGASRVKRTLAKTTRVVDRVLPWLRIPSV 305

Query: 317 PEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEPLKSDIP 376
           PEVF PFG+ FLYG+GGEGP A KL+KALC HAHNLAKERGCGVVATEVS+ EPLK  IP
Sbjct: 306 PEVFSPFGLHFLYGLGGEGPRAAKLVKALCGHAHNLAKERGCGVVATEVSSREPLKLGIP 365

Query: 377 HWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           HWK LSC EDLWCIKRLGE Y++ SLGDWTKSPP  SIFVDPREF
Sbjct: 366 HWKMLSCDEDLWCIKRLGEDYSDGSLGDWTKSPPGLSIFVDPREF 406

BLAST of Carg28224 vs. TAIR 10
Match: AT4G37580.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 547.4 bits (1409), Expect = 1.0e-155
Identity = 265/406 (65.27%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 18  LIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAEIAA 77
           ++RE+DP +D   VEDVERRCEVG   +   SLFTDLLGDPICR+RHSP++LMLVAE+  
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVG--PSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGT 62

Query: 78  H-NEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVSPDHRRLGI 137
              EIVGMIRGCIKTVTC  K   N   + S    + P+YTKLAY+LGLRVSP HRR GI
Sbjct: 63  EKKEIVGMIRGCIKTVTCGQKLDLN---HKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 138 GLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNPVFAHRLR 197
           G  LV+ MEEWFR+  AEYSYIATE DN AS+ LFT KCGYS+FRTPSILVNPV+AHR+ 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 198 LSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGS---FTDG 257
           +S +VT+++LE   AETLYR RF+T EFFPRDID+VL+N+LSLGTF+AVPRGS      G
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 258 LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPWLRLPS 317
            W  S +FL   PESWAVLS WNCKD + LEVRG S ++R +AK +RV+D+ LP+L+LPS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 318 VPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEPLKSDI 377
           +P VF+PFG+ F+YG+GGEGP A K++K+LC HAHNLAK  GCGVVA EV+ ++PL+  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 378 PHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           PHWK LSC EDLWCIKRLG+ Y++  +GDWTKSPP  SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of Carg28224 vs. TAIR 10
Match: AT2G23060.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 540.8 bits (1392), Expect = 9.6e-154
Identity = 263/413 (63.68%), Postives = 323/413 (78.21%), Query Frame = 0

Query: 15  IQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAE 74
           + + +RE+DP KD  +VEDVERRCEVG       SLFTDLLGDPICRVRHSP++LMLVAE
Sbjct: 3   VLVEVREYDPSKDLATVEDVERRCEVGPA--GKLSLFTDLLGDPICRVRHSPSYLMLVAE 62

Query: 75  IA--AHNEIVGMIRGCIKTVTCCSKSTR---NAGINASDLPQLAPVYTKLAYILGLRVSP 134
           I      E+VGMIRGCIKTVTC   + R       + +D+    P+YTKLAYILGLRVSP
Sbjct: 63  IGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSP 122

Query: 135 DHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNP 194
            HRR GIG  LV+ ME+WF +  AEYSY ATE DN AS+ LFT KCGY++FRTPSILVNP
Sbjct: 123 THRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNP 182

Query: 195 VFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGS 254
           V+AHR+ +S +VT+++LE + AE LYR RF+T EFFPRDID+VL+N+LSLGTF+AVPRGS
Sbjct: 183 VYAHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGS 242

Query: 255 -FTDG--LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGL 314
            +  G   W  S +FL   P+SWAVLS WNCKD + LEVRG S ++R ++K +R++D+ L
Sbjct: 243 CYGSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTL 302

Query: 315 PWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSND 374
           P+L++PS+P VF+PFG+ F+YG+GGEGP A K++KALC HAHNLAKE GCGVVA EV+ +
Sbjct: 303 PFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGE 362

Query: 375 EPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           EPL+  IPHWK LSC EDLWCIKRLGE Y++ S+GDWTKSPP  SIFVDPREF
Sbjct: 363 EPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of Carg28224 vs. TAIR 10
Match: AT2G23060.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.7e-132
Identity = 227/358 (63.41%), Postives = 280/358 (78.21%), Query Frame = 0

Query: 70  MLVAEIA--AHNEIVGMIRGCIKTVTCCSKSTR---NAGINASDLPQLAPVYTKLAYILG 129
           MLVAEI      E+VGMIRGCIKTVTC   + R       + +D+    P+YTKLAYILG
Sbjct: 1   MLVAEIGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILG 60

Query: 130 LRVSPDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPS 189
           LRVSP HRR GIG  LV+ ME+WF +  AEYSY ATE DN AS+ LFT KCGY++FRTPS
Sbjct: 61  LRVSPTHRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPS 120

Query: 190 ILVNPVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLA 249
           ILVNPV+AHR+ +S +VT+++LE + AE LYR RF+T EFFPRDID+VL+N+LSLGTF+A
Sbjct: 121 ILVNPVYAHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVA 180

Query: 250 VPRGS-FTDG--LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRV 309
           VPRGS +  G   W  S +FL   P+SWAVLS WNCKD + LEVRG S ++R ++K +R+
Sbjct: 181 VPRGSCYGSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRM 240

Query: 310 IDRGLPWLRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVAT 369
           +D+ LP+L++PS+P VF+PFG+ F+YG+GGEGP A K++KALC HAHNLAKE GCGVVA 
Sbjct: 241 VDKTLPFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAA 300

Query: 370 EVSNDEPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420
           EV+ +EPL+  IPHWK LSC EDLWCIKRLGE Y++ S+GDWTKSPP  SIFVDPREF
Sbjct: 301 EVAGEEPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 358

BLAST of Carg28224 vs. TAIR 10
Match: AT5G67430.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 434.5 bits (1116), Expect = 9.7e-122
Identity = 224/405 (55.31%), Postives = 290/405 (71.60%), Query Frame = 0

Query: 17  ILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAEIA 76
           +++RE+DP +D  SVE++E  CEVG       SL  DL+GDP+ R+R SP+F MLVAEI 
Sbjct: 8   VVVREYDPKRDLTSVEELEESCEVG-------SLLVDLMGDPLARIRQSPSFHMLVAEIG 67

Query: 77  AHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVSPDHRRLGI 136
             NEIVGMIRG IK VT    + R A   + ++       TKLA++ GLRVSP +RR+GI
Sbjct: 68  --NEIVGMIRGTIKMVTRGVNALRQADDVSPEINT-----TKLAFVSGLRVSPFYRRMGI 127

Query: 137 GLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNPVFAHRLR 196
           GL LV+R+EEWF    A YSY+ TE DN+AS+KLFT K GYSKFRTP+ LVNPVF HR+ 
Sbjct: 128 GLKLVQRLEEWFLRNDAVYSYVQTENDNIASVKLFTEKSGYSKFRTPTFLVNPVFNHRVT 187

Query: 197 LSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRGSFTDGLWV 256
           +S +V I++L  + AE+LYR RF+T EFFP DI+++L+N+LSLGT+LAVPRG        
Sbjct: 188 VSRRVKIIKLAPSDAESLYRNRFSTTEFFPSDINSILTNKLSLGTYLAVPRG-------- 247

Query: 257 ESDRFLSCLPE---SWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPWLRLPS 316
             D     LP+   SWAV+S WN KDVY L+V+G S +KR +AK +RV D   P+L++PS
Sbjct: 248 -GDNVSGSLPDQTGSWAVISIWNSKDVYRLQVKGASRLKRMLAKSTRVFDGAFPFLKIPS 307

Query: 317 VPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEPLKSDI 376
            P +FK F + F+YG+GGEGP A ++++ALC HAHNLA++ GC VVA EV++ EPL+  I
Sbjct: 308 FPNLFKSFAMHFMYGIGGEGPRAAEMVEALCSHAHNLARKSGCAVVAAEVASCEPLRVGI 367

Query: 377 PHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPRE 419
           PHWK LS PEDLWC+KRL   Y ++ + DWTKSPP  SIFVDPRE
Sbjct: 368 PHWKVLS-PEDLWCLKRL--RYDDDGV-DWTKSPPGLSIFVDPRE 385

BLAST of Carg28224 vs. TAIR 10
Match: AT2G30090.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 4.6e-79
Identity = 173/412 (41.99%), Postives = 239/412 (58.01%), Query Frame = 0

Query: 16  QILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAFLMLVAEI 75
           +++IR +D  +D+  +  +E+ CE+G   +    LFTD LGDPICR+R+SP F+MLVA +
Sbjct: 12  EVVIRCYDDRRDRIQMGRMEKSCEIG--HDHQTLLFTDTLGDPICRIRNSPFFIMLVAGV 71

Query: 76  AAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVSPDHRRLG 135
              N++VG I+G +K V    KS R                  + Y+LGLRV P +RR G
Sbjct: 72  G--NKLVGSIQGSVKPVEFHDKSVR------------------VGYVLGLRVVPSYRRRG 131

Query: 136 IGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVNPVFAHR- 195
           IG  LVR++EEWF    A+Y+Y+ATE DN AS  LF  + GY  FR P+ILVNPV   R 
Sbjct: 132 IGSILVRKLEEWFESHNADYAYMATEKDNEASHGLFIGRLGYVVFRNPAILVNPVNPGRG 191

Query: 196 LRLSDQVTILQLELTVAETLYRCRF-ATAEFFPRDIDAVLSNRLSLGTFLAVPRGSFTDG 255
           L+L   + I +L++  AE+LYR    AT EFFP DI+ +L N+LS+GT++A         
Sbjct: 192 LKLPSDIGIRKLKVKEAESLYRRNVAATTEFFPDDINKILRNKLSIGTWVAYYNN----- 251

Query: 256 LWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPWLRLPS 315
             V++ R       SWA+LS W+   V+ L +    +    + K+S++    L  L L  
Sbjct: 252 --VDNTR-------SWAMLSVWDSSKVFKLRIERAPLSYLLLTKVSKLFGNFLSLLGLTV 311

Query: 316 VPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKER---GCGVVATEV----SND 375
           +P++F PFG  FLYGV  EGP  GKL++ALC H HN+A       C VV  EV    + D
Sbjct: 312 LPDLFTPFGFYFLYGVHSEGPHCGKLVRALCEHVHNMAALNDGCACKVVVVEVDKGSNGD 371

Query: 376 EPLKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPRE 419
           + L+  IPHWK LSC +D+WCIK L        L + +KS    S+FVDPRE
Sbjct: 372 DSLQRCIPHWKMLSCDDDMWCIKPLKCEKNKFDLSERSKS--RSSLFVDPRE 385