Carg28212 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28212
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-H2 finger protein ATL13-like
Location	Carg_Chr03: 5259686 .. 5260610 (-)
RNA-Seq Expression	Carg28212
Synteny	Carg28212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCCATGCTTTTCACACACAATGTATTGACACATGGCTTTTGTCTCACTCAACCTGCCCTCTCTGTAGATCTAGTTTGCTACCTGATCATTTCTCCCCACACACCACTCGTGCACCAATTGTTCTTGTTCTTGAATCCGGGAGCGAGAGTTCTGTAAACAGAGAGGGTACATCCGGTAGAACAAATCCGATTTCTGGGTCAAATTCGCAAGTGGGTCTTGGTGGAAATGGTGAATTTGAGGCAGGGGCGAGCGAGATCATGAAATTAGAGGATTCAAACCCAAGTGTAGTGACTGTGAAGCTTGGAAAATACAAAAACATCGATGCAGGGGAAGGAGGAAGTAGCGAGAACAAGAATGTGGATTCTCGTAGGTGCTTTTCAATGGGGGTTTTTGAATATGTAATGGACGAACATTCGTCTCTCACAGTAGCGATTAGAAGCAAGCCAGTGAAGAAGCATCCAAGCAGGAAGCCAGCTCTGCCGTCGACGCCTGGTTACAGAGCAGCCATGTCCGAATGCGACTGCGAATCCAGAAGAGAGTTCAAAATCAGTGGGATTGAAGCTATTGGGGAAGTGAAAAAGGGAGAGATTACTGATACAAACAACAACAAAATGGAGAGTTTCTCTGTATCGAAGATTTGGCTGAGAGGAAAAACCCGGAGAGAATCGGCCGCAGATTCTTCAAGAAGGGCAGTTTCACTACGGTTTCCGATGCAGAGCAACCGTGGGGATCTCTCAGAGATGAACGTTTGGAGGTGGGAAAATGACGTCGGAGACGAAGAAAACCAGAGGCGTACCAGCTTCGAGTCTGAACTGAATCAATCTTTCGCGAAGAGGACGTTACAATGGCTGATGGGAGGAAGACAAAACAAGGCGACACACTTTGAATCATCATCTTCCTCCTCTGTTTCAAATATCTAA

mRNA sequence

ATGCAGCCATGCTTTTCACACACAATATCTAGTTTGCTACCTGATCATTTCTCCCCACACACCACTCGTGCACCAATTGTTCTTGTTCTTGAATCCGGGAGCGAGAGTTCTGTAAACAGAGAGGGTACATCCGGTAGAACAAATCCGATTTCTGGGTCAAATTCGCAAGTGGGTCTTGGTGGAAATGGTGAATTTGAGGCAGGGGCGAGCGAGATCATGAAATTAGAGGATTCAAACCCAAGTGTAGTGACTGTGAAGCTTGGAAAATACAAAAACATCGATGCAGGGGAAGGAGGAAGTAGCGAGAACAAGAATGTGGATTCTCGTAGGTGCTTTTCAATGGGGGTTTTTGAATATGTAATGGACGAACATTCGTCTCTCACAGTAGCGATTAGAAGCAAGCCAGTGAAGAAGCATCCAAGCAGGAAGCCAGCTCTGCCGTCGACGCCTGGTTACAGAGCAGCCATGTCCGAATGCGACTGCGAATCCAGAAGAGAGTTCAAAATCAGTGGGATTGAAGCTATTGGGGAAGTGAAAAAGGGAGAGATTACTGATACAAACAACAACAAAATGGAGAGTTTCTCTGTATCGAAGATTTGGCTGAGAGGAAAAACCCGGAGAGAATCGGCCGCAGATTCTTCAAGAAGGGCAGTTTCACTACGGTTTCCGATGCAGAGCAACCGTGGGGATCTCTCAGAGATGAACGTTTGGAGGTGGGAAAATGACGTCGGAGACGAAGAAAACCAGAGGCGTACCAGCTTCGAGTCTGAACTGAATCAATCTTTCGCGAAGAGGACGTTACAATGGCTGATGGGAGGAAGACAAAACAAGGCGACACACTTTGAATCATCATCTTCCTCCTCTGTTTCAAATATCTAA

Coding sequence (CDS)

ATGCAGCCATGCTTTTCACACACAATATCTAGTTTGCTACCTGATCATTTCTCCCCACACACCACTCGTGCACCAATTGTTCTTGTTCTTGAATCCGGGAGCGAGAGTTCTGTAAACAGAGAGGGTACATCCGGTAGAACAAATCCGATTTCTGGGTCAAATTCGCAAGTGGGTCTTGGTGGAAATGGTGAATTTGAGGCAGGGGCGAGCGAGATCATGAAATTAGAGGATTCAAACCCAAGTGTAGTGACTGTGAAGCTTGGAAAATACAAAAACATCGATGCAGGGGAAGGAGGAAGTAGCGAGAACAAGAATGTGGATTCTCGTAGGTGCTTTTCAATGGGGGTTTTTGAATATGTAATGGACGAACATTCGTCTCTCACAGTAGCGATTAGAAGCAAGCCAGTGAAGAAGCATCCAAGCAGGAAGCCAGCTCTGCCGTCGACGCCTGGTTACAGAGCAGCCATGTCCGAATGCGACTGCGAATCCAGAAGAGAGTTCAAAATCAGTGGGATTGAAGCTATTGGGGAAGTGAAAAAGGGAGAGATTACTGATACAAACAACAACAAAATGGAGAGTTTCTCTGTATCGAAGATTTGGCTGAGAGGAAAAACCCGGAGAGAATCGGCCGCAGATTCTTCAAGAAGGGCAGTTTCACTACGGTTTCCGATGCAGAGCAACCGTGGGGATCTCTCAGAGATGAACGTTTGGAGGTGGGAAAATGACGTCGGAGACGAAGAAAACCAGAGGCGTACCAGCTTCGAGTCTGAACTGAATCAATCTTTCGCGAAGAGGACGTTACAATGGCTGATGGGAGGAAGACAAAACAAGGCGACACACTTTGAATCATCATCTTCCTCCTCTGTTTCAAATATCTAA

Protein sequence

MQPCFSHTISSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGASEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI

Homology

[Close]

BLAST of Carg28212 vs. NCBI nr
Match: KAG7033987.1 (RING-H2 finger protein ATL13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 571.2 bits (1471), Expect = 5.0e-159
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0


Query: 1   MQPCFSHTISSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLG 60
           MQPCFSHTISSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLG
Sbjct: 1   MQPCFSHTISSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLG 60

Query: 61  GNGEFEAGASEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYV 120
           GNGEFEAGASEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYV
Sbjct: 61  GNGEFEAGASEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYV 120

Query: 121 MDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKK 180
           MDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKK
Sbjct: 121 MDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKK 180

Query: 181 GEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWE 240
           GEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWE
Sbjct: 181 GEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWE 240

Query: 241 NDVGDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           NDVGDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 241 NDVGDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 292

[Close]

BLAST of Carg28212 vs. NCBI nr
Match: KAG6589737.1 (RING-H2 finger protein ATL13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545.4 bits (1404), Expect = 2.9e-151
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA
Sbjct: 169 SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 228

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEIMKLEDSNPSVVTVKLGKYKNIDAGEG SSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 229 SEIMKLEDSNPSVVTVKLGKYKNIDAGEGRSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 288

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN
Sbjct: 289 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 348

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ
Sbjct: 349 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 408

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 409 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 451

[Close]

BLAST of Carg28212 vs. NCBI nr
Match: XP_022988578.1 (RING-H2 finger protein ATL13-like [Cucurbita maxima])

HSP 1 Score: 531.2 bits (1367), Expect = 5.7e-147
Identity = 272/283 (96.11%), Postives = 276/283 (97.53%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPH TRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA
Sbjct: 164 SSLLPDHFSPHATRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 223

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEI KLEDSNPS+VTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 224 SEIRKLEDSNPSIVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 283

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIE IGEVK+GEITD  NN
Sbjct: 284 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEGIGEVKRGEITDRTNN 343

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFP+QSNRGDLSEM+VWRWENDVGDEENQ
Sbjct: 344 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPIQSNRGDLSEMDVWRWENDVGDEENQ 403

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RR SFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 404 RRNSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 446

[Close]

BLAST of Carg28212 vs. NCBI nr
Match: XP_022921806.1 (RING-H2 finger protein ATL13-like [Cucurbita moschata])

HSP 1 Score: 530.0 bits (1364), Expect = 1.3e-146
Identity = 274/283 (96.82%), Postives = 277/283 (97.88%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG    TNPISGSNSQVG+GGNGEFE GA
Sbjct: 169 SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG----TNPISGSNSQVGVGGNGEFETGA 228

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 229 SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 288

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRSKP+KKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN
Sbjct: 289 AIRSKPMKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 348

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFP+QSNRGDLSEMNVWRWENDVGDEENQ
Sbjct: 349 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPIQSNRGDLSEMNVWRWENDVGDEENQ 408

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 409 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 447

[Close]

BLAST of Carg28212 vs. NCBI nr
Match: XP_023515811.1 (RING-H2 finger protein ATL13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.2 bits (1323), Expect = 7.2e-142
Identity = 267/283 (94.35%), Postives = 272/283 (96.11%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG    TN ISGSNSQVGLGGNGEFE GA
Sbjct: 167 SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG----TNSISGSNSQVGLGGNGEFETGA 226

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 227 SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 286

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIR  PVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKK EITDTNNN
Sbjct: 287 AIRRNPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKEEITDTNNN 346

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKT+RESAADSSRRAVSLRFP+QSNRG+LSEM+VWRWEND+GDEENQ
Sbjct: 347 KMESFSVSKIWLRGKTQRESAADSSRRAVSLRFPIQSNRGNLSEMDVWRWENDIGDEENQ 406

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RRTSFESELNQSFAKRTLQWLMGGRQNKATH ESSSSSSVSNI
Sbjct: 407 RRTSFESELNQSFAKRTLQWLMGGRQNKATHLESSSSSSVSNI 445

[Close]

BLAST of Carg28212 vs. ExPASy Swiss-Prot
Match: Q940Q4 (RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 5.0e-29
Identity = 121/317 (38.17%), Postives = 162/317 (51.10%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTS-------GRTNPISGSNSQVGLGGN 69
           SSLL D  S    R+  +LVLES S+ S    G            + I  S++ +GL GN
Sbjct: 177 SSLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLVGN 236

Query: 70  GEFEAGASEI----------MKLEDSNPSVV--TVKLGKYKNIDAGEGGSSEN-----KN 129
            +   G++ I           +L  S   VV  +VKLGK++NID GEG SS N      +
Sbjct: 237 NDL--GSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIGEGTSSNNNIGNSSS 296

Query: 130 VDSRRCFSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRR 189
           +D RRCFSMG +EY+MDE ++L V + +   KK  S+   L   PG+R AMSEC  +   
Sbjct: 297 LDERRCFSMGSYEYIMDEETTLKVHVST---KKQSSKNRGL---PGHRTAMSECGFDPTG 356

Query: 190 EFKISGIEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRES----------AADSSR 249
             K SG  ++  V+  E  + N  + ESFSVSKIWLRGK  + S           + SS 
Sbjct: 357 RLKFSGSGSMRIVE--EAAEKNVVERESFSVSKIWLRGKKEKHSKVQGKEDSSLVSSSSG 416

Query: 250 RAVSLRFPMQSNRGD-LSEMNVWRWENDVGDEENQRRTSFES--ELNQSFAKRTLQWLMG 290
           RA S R   Q N  D + E           +E+NQ+  + ES      SFA+RT+ WL  
Sbjct: 417 RAFSFRLSNQRNHPDAMIESGC--------EEDNQKCENSESLETKTPSFARRTMLWL-A 472

[Close]

BLAST of Carg28212 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 103/302 (34.11%), Postives = 141/302 (46.69%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEA-- 69
           S+LL    S H   +  +LVLES        E +S    P+  SNSQ+G   N + E+  
Sbjct: 169 SNLLSGFSSHHNLSSSYLLVLES--------EQSSRDMVPVLESNSQLGYDVNNDSESTR 228

Query: 70  --------GASEIMKLEDSNPSVVTVKLGKYKNID-AGEGG-------SSENKNVDSRRC 129
                        M   D     + VKLGK++NID  GEG        S  +KNVD RRC
Sbjct: 229 IRSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRNIDHVGEGSDQKKNSISGNSKNVDGRRC 288

Query: 130 FSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISG 189
            SMG +EY+MD+ ++L V + +K +    S K  +PS   +R  MSEC  +      + G
Sbjct: 289 LSMGSYEYIMDQEATLKVHVSTKKL----SGKDRVPS---HRTVMSECGFDP----TVKG 348

Query: 190 IEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRE---SAADSSRRAV---SLRFPMQ 249
           IE      K  +      + ESFS+SKIWLRGK  ++   SA DS    V   SLRFP  
Sbjct: 349 IE------KSVV------ERESFSLSKIWLRGKKEKQKGTSARDSDCSFVSSSSLRFP-- 408

Query: 250 SNRGDLSEMNVWRWENDVGDEENQRRTSFES--ELNQSFAKRTLQWLMGGRQNKATHFES 286
                          + +  EE+ +  + ES      SFA+RT+ WL  GRQNK     +
Sbjct: 409 --------------NHRIPPEESLKSENSESLETKTPSFARRTMHWL-AGRQNKIVQPST 422

[Close]

BLAST of Carg28212 vs. ExPASy Swiss-Prot
Match: Q9FL07 (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.0e-05
Identity = 50/168 (29.76%), Postives = 77/168 (45.83%), Query Frame = 0


Query: 63  GEFEAGASEIMKLED--SNPSVVTVKLGKYKNI---------DAGEGGSSENKNVDSRRC 122
           G  E G+ + M++++      V+ V+LGK+K +         D   GG + + N+D+RRC
Sbjct: 211 GVTENGSQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETSSSNLDARRC 270

Query: 123 FSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISG 182
           FSMG ++Y++  +S L V          P     LP     R    + + E         
Sbjct: 271 FSMGSYQYILG-NSELKV----------PFANDRLP-----RLKPQDKESEQTGNSSSED 330

Query: 183 IEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVS 220
            + I  V KG          ESFSVSKIWL  K + + ++D+ RR  S
Sbjct: 331 NKKINTVAKG----------ESFSVSKIWLWPK-KDKFSSDAQRRLPS 351

[Close]

BLAST of Carg28212 vs. ExPASy TrEMBL
Match: A0A6J1JMP8 (RING-H2 finger protein ATL13-like OS=Cucurbita maxima OX=3661 GN=LOC111485778 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.8e-147
Identity = 272/283 (96.11%), Postives = 276/283 (97.53%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPH TRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA
Sbjct: 164 SSLLPDHFSPHATRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 223

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEI KLEDSNPS+VTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 224 SEIRKLEDSNPSIVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 283

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIE IGEVK+GEITD  NN
Sbjct: 284 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEGIGEVKRGEITDRTNN 343

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFP+QSNRGDLSEM+VWRWENDVGDEENQ
Sbjct: 344 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPIQSNRGDLSEMDVWRWENDVGDEENQ 403

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RR SFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 404 RRNSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 446

[Close]

BLAST of Carg28212 vs. ExPASy TrEMBL
Match: A0A6J1E2E3 (RING-H2 finger protein ATL13-like OS=Cucurbita moschata OX=3662 GN=LOC111429949 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.2e-147
Identity = 274/283 (96.82%), Postives = 277/283 (97.88%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG    TNPISGSNSQVG+GGNGEFE GA
Sbjct: 169 SSLLPDHFSPHTTRAPIVLVLESGSESSVNREG----TNPISGSNSQVGVGGNGEFETGA 228

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSL V
Sbjct: 229 SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLKV 288

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRSKP+KKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN
Sbjct: 289 AIRSKPMKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 348

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGDLSEMNVWRWENDVGDEENQ 249
           KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFP+QSNRGDLSEMNVWRWENDVGDEENQ
Sbjct: 349 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPIQSNRGDLSEMNVWRWENDVGDEENQ 408

Query: 250 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 293
           RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI
Sbjct: 409 RRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVSNI 447

[Close]

BLAST of Carg28212 vs. ExPASy TrEMBL
Match: A0A6J1IDU5 (RING-H2 finger protein ATL13-like OS=Cucurbita maxima OX=3661 GN=LOC111472249 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.9e-106
Identity = 212/292 (72.60%), Postives = 243/292 (83.22%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHT+ APIVLVLESGSESS +    +  TNPI+GSNSQVGLGG+ EF+AG+
Sbjct: 172 SSLLPDHFSPHTSCAPIVLVLESGSESSRDNVSNTQATNPITGSNSQVGLGGDSEFDAGS 231

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEI KLEDS  +VVTVKLGKYKNIDAGEGGSSENKN+DSRRCFSMG FEYVM+E+SSL V
Sbjct: 232 SEIGKLEDSKATVVTVKLGKYKNIDAGEGGSSENKNMDSRRCFSMGSFEYVMEENSSLKV 291

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRS P KK PSRKPALP TPGYRAAMSEC+CESRREF++SGIE   EV++GE TD N+ 
Sbjct: 292 AIRSTPAKKQPSRKPALPLTPGYRAAMSECECESRREFRLSGIEPFREVRRGENTDRNSK 351

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGD--LSEMNVWRWENDV---- 249
           K++SFSVSKIWLRGK +RE++ +SSRRAVS RFP+Q N GD   SEM V RWEN++    
Sbjct: 352 KIDSFSVSKIWLRGKNQRETSTESSRRAVSFRFPIQKNGGDWSASEMGVSRWENEIIRSE 411

Query: 250 --GDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSS-SSSVSNI 293
              DEENQ R SF+S+ NQSFAKRTL WLM G+QNK THFESSS SS +SN+
Sbjct: 412 TELDEENQSRCSFDSQFNQSFAKRTLLWLM-GKQNKVTHFESSSPSSCISNL 462

[Close]

BLAST of Carg28212 vs. ExPASy TrEMBL
Match: A0A6J1ESY3 (RING-H2 finger protein ATL13-like OS=Cucurbita moschata OX=3662 GN=LOC111435713 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.1e-103
Identity = 211/292 (72.26%), Postives = 238/292 (81.51%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEAGA 69
           SSLLPDHFSPHT+ APIVLVLESGSESS +    +  TNPI+GSNSQVGLGG+ EF+AG+
Sbjct: 172 SSLLPDHFSPHTSYAPIVLVLESGSESSRDNVSNTEATNPITGSNSQVGLGGDSEFDAGS 231

Query: 70  SEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTV 129
           SEI KLEDS  +VVTVKLGKYKNIDAGEGGSS NKN+DSRRCFSM  FEYVM+E+SSL V
Sbjct: 232 SEIGKLEDSKATVVTVKLGKYKNIDAGEGGSSGNKNMDSRRCFSMESFEYVMEENSSLKV 291

Query: 130 AIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDTNNN 189
           AIRS P KK PSRKPALP TPGYRAAMSECDCESRREF++SGIE   EVK+GE TD N  
Sbjct: 292 AIRSTPAKKQPSRKPALPLTPGYRAAMSECDCESRREFRLSGIEPFREVKRGENTDRNCK 351

Query: 190 KMESFSVSKIWLRGKTRRESAADSSRRAVSLRFPMQSNRGD--LSEMNVWRWENDV---- 249
           K++SFSVSKIWLRGK +RE+  +SSRRAVS RFP+Q N GD   SEM V RWEN++    
Sbjct: 352 KIDSFSVSKIWLRGKNQRETPTESSRRAVSFRFPIQKNGGDWNASEMGVSRWENEIIRNE 411

Query: 250 --GDEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSS-SSSVSNI 293
              DEENQ R S +S+ NQSFAKRTL WLM G+QNK THFESSS SS +SN+
Sbjct: 412 TELDEENQSRCSLDSQFNQSFAKRTLVWLM-GKQNKVTHFESSSPSSCISNL 462

[Close]

BLAST of Carg28212 vs. ExPASy TrEMBL
Match: A0A5D3BAM4 (RING-H2 finger protein ATL13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00060 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.3e-96
Identity = 203/287 (70.73%), Postives = 228/287 (79.44%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESS---VNREGTSGRTNPISGSNSQVGLGGNGEFE 69
           SSLLPDHFSPHTT +PIVLVLESGSESS   V+    +  TN I GSNS++G  G+GEFE
Sbjct: 172 SSLLPDHFSPHTTCSPIVLVLESGSESSRDIVSDREITNHTNQIPGSNSRMGFVGDGEFE 231

Query: 70  AGASEIMKLEDSNPSVVTVKLGKYKNIDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSS 129
            G+SEI KLEDSN +VVTVKLGKYKN+DAGE GSSENKNVDSRRCFSMG FEYVMDE+SS
Sbjct: 232 GGSSEITKLEDSNATVVTVKLGKYKNVDAGESGSSENKNVDSRRCFSMGSFEYVMDENSS 291

Query: 130 LTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISGIEAIGEVKKGEITDT 189
           + VAIRS PVKK  SRKPALP TPGYRAAMSE DCESRREFK++GIE +   + G  +++
Sbjct: 292 VKVAIRSTPVKKQASRKPALPLTPGYRAAMSEYDCESRREFKLNGIEPLKTEENGN-SNS 351

Query: 190 NNNKMESFSVSKIWLRGKTRRESAADSS-RRAVSLRFPMQSNRGDLSEMNVWRWENDVG- 249
           NNNK+ESFSVSKIWLRGK  +ES  DSS RR VS R P+Q N    SEMNV RWEN+   
Sbjct: 352 NNNKIESFSVSKIWLRGKNPKESLTDSSRRRTVSFRLPIQMN----SEMNVCRWENESME 411

Query: 250 -DEENQRRTSFESELNQSFAKRTLQWLMGGRQNKATHFESSSSSSVS 291
            DEENQ R S ES+ NQS AKRTLQWLMGG+ NK THFES+SS S S
Sbjct: 412 LDEENQSRNSLESQYNQSLAKRTLQWLMGGKLNKVTHFESTSSPSPS 453

[Close]

BLAST of Carg28212 vs. TAIR 10
Match: AT4G30400.1 (RING/U-box superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 3.6e-30
Identity = 121/317 (38.17%), Postives = 162/317 (51.10%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTS-------GRTNPISGSNSQVGLGGN 69
           SSLL D  S    R+  +LVLES S+ S    G            + I  S++ +GL GN
Sbjct: 177 SSLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLVGN 236

Query: 70  GEFEAGASEI----------MKLEDSNPSVV--TVKLGKYKNIDAGEGGSSEN-----KN 129
            +   G++ I           +L  S   VV  +VKLGK++NID GEG SS N      +
Sbjct: 237 NDL--GSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIGEGTSSNNNIGNSSS 296

Query: 130 VDSRRCFSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRR 189
           +D RRCFSMG +EY+MDE ++L V + +   KK  S+   L   PG+R AMSEC  +   
Sbjct: 297 LDERRCFSMGSYEYIMDEETTLKVHVST---KKQSSKNRGL---PGHRTAMSECGFDPTG 356

Query: 190 EFKISGIEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRES----------AADSSR 249
             K SG  ++  V+  E  + N  + ESFSVSKIWLRGK  + S           + SS 
Sbjct: 357 RLKFSGSGSMRIVE--EAAEKNVVERESFSVSKIWLRGKKEKHSKVQGKEDSSLVSSSSG 416

Query: 250 RAVSLRFPMQSNRGD-LSEMNVWRWENDVGDEENQRRTSFES--ELNQSFAKRTLQWLMG 290
           RA S R   Q N  D + E           +E+NQ+  + ES      SFA+RT+ WL  
Sbjct: 417 RAFSFRLSNQRNHPDAMIESGC--------EEDNQKCENSESLETKTPSFARRTMLWL-A 472

[Close]

BLAST of Carg28212 vs. TAIR 10
Match: AT2G18650.1 (RING/U-box superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 7.5e-20
Identity = 103/302 (34.11%), Postives = 141/302 (46.69%), Query Frame = 0


Query: 10  SSLLPDHFSPHTTRAPIVLVLESGSESSVNREGTSGRTNPISGSNSQVGLGGNGEFEA-- 69
           S+LL    S H   +  +LVLES        E +S    P+  SNSQ+G   N + E+  
Sbjct: 169 SNLLSGFSSHHNLSSSYLLVLES--------EQSSRDMVPVLESNSQLGYDVNNDSESTR 228

Query: 70  --------GASEIMKLEDSNPSVVTVKLGKYKNID-AGEGG-------SSENKNVDSRRC 129
                        M   D     + VKLGK++NID  GEG        S  +KNVD RRC
Sbjct: 229 IRSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRNIDHVGEGSDQKKNSISGNSKNVDGRRC 288

Query: 130 FSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISG 189
            SMG +EY+MD+ ++L V + +K +    S K  +PS   +R  MSEC  +      + G
Sbjct: 289 LSMGSYEYIMDQEATLKVHVSTKKL----SGKDRVPS---HRTVMSECGFDP----TVKG 348

Query: 190 IEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRE---SAADSSRRAV---SLRFPMQ 249
           IE      K  +      + ESFS+SKIWLRGK  ++   SA DS    V   SLRFP  
Sbjct: 349 IE------KSVV------ERESFSLSKIWLRGKKEKQKGTSARDSDCSFVSSSSLRFP-- 408

Query: 250 SNRGDLSEMNVWRWENDVGDEENQRRTSFES--ELNQSFAKRTLQWLMGGRQNKATHFES 286
                          + +  EE+ +  + ES      SFA+RT+ WL  GRQNK     +
Sbjct: 409 --------------NHRIPPEESLKSENSESLETKTPSFARRTMHWL-AGRQNKIVQPST 422

[Close]

BLAST of Carg28212 vs. TAIR 10
Match: AT5G40250.1 (RING/U-box superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 7.3e-07
Identity = 50/168 (29.76%), Postives = 77/168 (45.83%), Query Frame = 0


Query: 63  GEFEAGASEIMKLED--SNPSVVTVKLGKYKNI---------DAGEGGSSENKNVDSRRC 122
           G  E G+ + M++++      V+ V+LGK+K +         D   GG + + N+D+RRC
Sbjct: 211 GVTENGSQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETSSSNLDARRC 270

Query: 123 FSMGVFEYVMDEHSSLTVAIRSKPVKKHPSRKPALPSTPGYRAAMSECDCESRREFKISG 182
           FSMG ++Y++  +S L V          P     LP     R    + + E         
Sbjct: 271 FSMGSYQYILG-NSELKV----------PFANDRLP-----RLKPQDKESEQTGNSSSED 330

Query: 183 IEAIGEVKKGEITDTNNNKMESFSVSKIWLRGKTRRESAADSSRRAVS 220
            + I  V KG          ESFSVSKIWL  K + + ++D+ RR  S
Sbjct: 331 NKKINTVAKG----------ESFSVSKIWLWPK-KDKFSSDAQRRLPS 351

[Close]

BLAST of Carg28212 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.6e-04
Identity = 22/58 (37.93%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 82  VVTVKLGKYKN--------IDAGEGGSSENKNVDSRRCFSMGVFEYVMDEHSSLTVAI 132
           V +V+LGK+++        +  G GG + + ++D+RRCFSMG ++Y++ E S L VA+
Sbjct: 232 VFSVRLGKFRSSNIVNNGEVVVGGGGETSSSSLDNRRCFSMGSYQYIVAE-SDLVVAL 288

The following BLAST results are available for this feature:

BLAST of Carg28212 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033987.1	5.0e-159	100.00	RING-H2 finger protein ATL13, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6589737.1	2.9e-151	99.29	RING-H2 finger protein ATL13, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022988578.1	5.7e-147	96.11	RING-H2 finger protein ATL13-like [Cucurbita maxima]	[more]
XP_022921806.1	1.3e-146	96.82	RING-H2 finger protein ATL13-like [Cucurbita moschata]	[more]
XP_023515811.1	7.2e-142	94.35	RING-H2 finger protein ATL13-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg28212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940Q4	5.0e-29	38.17	RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2	[more]
Q9ZV53	1.1e-18	34.11	Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 P...	[more]
Q9FL07	1.0e-05	29.76	RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1	[more]

BLAST of Carg28212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JMP8	2.8e-147	96.11	RING-H2 finger protein ATL13-like OS=Cucurbita maxima OX=3661 GN=LOC111485778 PE...	[more]
A0A6J1E2E3	6.2e-147	96.82	RING-H2 finger protein ATL13-like OS=Cucurbita moschata OX=3662 GN=LOC111429949 ...	[more]
A0A6J1IDU5	6.9e-106	72.60	RING-H2 finger protein ATL13-like OS=Cucurbita maxima OX=3661 GN=LOC111472249 PE...	[more]
A0A6J1ESY3	1.1e-103	72.26	RING-H2 finger protein ATL13-like OS=Cucurbita moschata OX=3662 GN=LOC111435713 ...	[more]
A0A5D3BAM4	1.3e-96	70.73	RING-H2 finger protein ATL13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Carg28212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30400.1	3.6e-30	38.17	RING/U-box superfamily protein	[more]
AT2G18650.1	7.5e-20	34.11	RING/U-box superfamily protein	[more]
AT5G40250.1	7.3e-07	29.76	RING/U-box superfamily protein	[more]
AT1G23980.1	2.6e-04	37.93	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 134..153
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..60
None	No IPR available	PANTHER	PTHR45768:SF53	ZINC FINGER, RING/FYVE/PHD-TYPE-RELATED	coord: 13..213
None	No IPR available	PANTHER	PTHR45768	E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE	coord: 13..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28212-RA	Carg28212-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane