Carg28184 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28184
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Location	Carg_Chr09: 12265755 .. 12267433 (-)
RNA-Seq Expression	Carg28184
Synteny	Carg28184

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGTTTACCGTACAGGTGAGCAGCAGAGGATGAGGGATTGGCTTCCACTGTGGAAAAGAGCCGAGAGGAGGAACAATCTGGTCCCTGTGAGTGACCCTTTCCAAAGATAGAATTTCTCCACCAAAAGCAGCGGCGGTGTTTTCCTTTTCTTCTCTCTTCCAGAAATAACGAAAAACAACAAAATTCAGCAGAAATCTCCTGAGCCAGTCACTGTGAAGCAGATGCAATCCACAACTGGTGGTGCTACTGGCAGTCGTAGCGGCGGCGGCGGTAGCGGCGGCGGACTTGCTCGCTTCCGCTCTGCTCCGGCTGCCTGGTTGGAAGCGCTTTTGGAGGACGAGGAAGAGGACCTACTCAGGCCTAATCAGTGCTTGACTCAGCTTCTCGCTGGCAACTCCTCCGACCTAGACTCCCCCGCGGATCAGGCCTTGTTCGAGCCCAATCCCTCCGCTGGTTTCCAGAGGCAAAATAGCTCTCCGGCTGAGTTTCTCGGCGCCTCCGGAATCGGGGAAGGATTTTACTCTTCCTATGCTATCAATTCCTCGGCCGCTCTCGAGATCTCTCCCACTTCCAAGCGGGGGAGAGAAGTCGACGCTCAGAACTTCCCGCCTAAATTTTCGCCGCAGCTGGTAATCATTTTATTTCGAACCTCTGTTTTGCGATTCCCTGTTTGGAGGATTTGAAACGATTTTGATTTGGGATTACTTTTCTCTTGATTATGGTCTCCCGATTAGAAGTCGTTTTTGAAATTGTTTAAGTGAAATTTTTGGCAGAAAAGGGAAGGAAGTGGAGTATCGAGTTTGATAGACATGGAAATGGAGAAGTTATTGGAGGACTCCGTGCCCTGCCGTGTGAGGGCCAAGCGTGGCTGTGCGACGCATCCTCGGAGCATTGCTGAGAGGGTTAGTTCCTTTTCGCCTAAATCTTCGTTGACTAATTGCTTCTTTCAACTTCCACTTTTCAATACCCAATCTCACTTGTTCATAACTTCTGTGACATTCTTCACTGTAATAATTCCACATGGATTCCTTGCCTCTTCTGATCATTCATCATCTCAGCTCTGCCTTTATGTCTATTTCAAACTTACATTTAAAATGTTTTCTATCACACTCTTGTAAACAATGATTCGTTTTCCTCCCCAACCGACGTAGGATCTCACAATCCACCCACCTATCACTCGTTCCCTTCTCCAATCGATGTAGGACCCTCCAATCCACCCCTATGAGGCCAGCATCTTTGCTGGCACACTGTCTCGTGTCCACTCCCTCTTGGAGCTCAGTCTCCCTGCTGACACATCGCTCAGTGTCTAGCTTTGATACCATCTAGCTTTGATACCATCTGTAACGGCTCAAGCCCACCCTAGTAGATATTGTCTTTTCCTTTCTTTGTTGGCCTTTCTTTGTGTTAAAACTATTTACTCATTAGACTAAGCAGTATGTCTTGTGAACAAAAGGTATGTTGATTACTTATGTTTTGTTTTCTTGATGGTAGGTTCGTAGGACTAGAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTCCCCAACATGGATAAGGTAAATTATATCATGATTGATCATTAGGGACGAATTTAGATTTATCTTATGTGATATATATTGATAAATGACTGTATTTTGTACTGCCATGTGTTTCTTCTTCCAGTTTCTGCCCTGA

mRNA sequence

ATGGGCGTTTACCGTACAGGTGAGCAGCAGAGGATGAGGGATTGGCTTCCACTGTGGAAAAGAGCCGAGAGGAGGAACAATCTGGTCCCTCAGAAATCTCCTGAGCCAGTCACTGTGAAGCAGATGCAATCCACAACTGGTGGTGCTACTGGCAGTCGTAGCGGCGGCGGCGGTAGCGGCGGCGGACTTGCTCGCTTCCGCTCTGCTCCGGCTGCCTGGTTGGAAGCGCTTTTGGAGGACGAGGAAGAGGACCTACTCAGGCCTAATCAGTGCTTGACTCAGCTTCTCGCTGGCAACTCCTCCGACCTAGACTCCCCCGCGGATCAGGCCTTGTTCGAGCCCAATCCCTCCGCTGGTTTCCAGAGGCAAAATAGCTCTCCGGCTGAGTTTCTCGGCGCCTCCGGAATCGGGGAAGGATTTTACTCTTCCTATGCTATCAATTCCTCGGCCGCTCTCGAGATCTCTCCCACTTCCAAGCGGGGGAGAGAAGTCGACGCTCAGAACTTCCCGCCTAAATTTTCGCCGCAGCTGAAAAGGGAAGGAAGTGGAGTATCGAGTTTGATAGACATGGAAATGGAGAAGTTATTGGAGGACTCCGTGCCCTGCCGTGTGAGGGCCAAGCGTGGCTGTGCGACGCATCCTCGGAGCATTGCTGAGAGGGTTCGTAGGACTAGAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTCCCCAACATGGATAAGTTTCTGCCCTGA

Coding sequence (CDS)

ATGGGCGTTTACCGTACAGGTGAGCAGCAGAGGATGAGGGATTGGCTTCCACTGTGGAAAAGAGCCGAGAGGAGGAACAATCTGGTCCCTCAGAAATCTCCTGAGCCAGTCACTGTGAAGCAGATGCAATCCACAACTGGTGGTGCTACTGGCAGTCGTAGCGGCGGCGGCGGTAGCGGCGGCGGACTTGCTCGCTTCCGCTCTGCTCCGGCTGCCTGGTTGGAAGCGCTTTTGGAGGACGAGGAAGAGGACCTACTCAGGCCTAATCAGTGCTTGACTCAGCTTCTCGCTGGCAACTCCTCCGACCTAGACTCCCCCGCGGATCAGGCCTTGTTCGAGCCCAATCCCTCCGCTGGTTTCCAGAGGCAAAATAGCTCTCCGGCTGAGTTTCTCGGCGCCTCCGGAATCGGGGAAGGATTTTACTCTTCCTATGCTATCAATTCCTCGGCCGCTCTCGAGATCTCTCCCACTTCCAAGCGGGGGAGAGAAGTCGACGCTCAGAACTTCCCGCCTAAATTTTCGCCGCAGCTGAAAAGGGAAGGAAGTGGAGTATCGAGTTTGATAGACATGGAAATGGAGAAGTTATTGGAGGACTCCGTGCCCTGCCGTGTGAGGGCCAAGCGTGGCTGTGCGACGCATCCTCGGAGCATTGCTGAGAGGGTTCGTAGGACTAGAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTCCCCAACATGGATAAGTTTCTGCCCTGA

Protein sequence

MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKFLP

Homology

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BLAST of Carg28184 vs. NCBI nr
Match: KAG7025210.1 (Transcription factor bHLH80, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478.0 bits (1229), Expect = 4.8e-131
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG 60
           MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG
Sbjct: 1   MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG 60

Query: 61  GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF 120
           GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF
Sbjct: 61  GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF 120

Query: 121 QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE 180
           QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE
Sbjct: 121 QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE 180

Query: 181 GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM 240
           GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM
Sbjct: 181 GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM 240

Query: 241 DKFLP 246
           DKFLP
Sbjct: 241 DKFLP 245

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BLAST of Carg28184 vs. NCBI nr
Match: KAG6592393.1 (Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471.1 bits (1211), Expect = 5.9e-129
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG 60
           MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG
Sbjct: 1   MGVYRTGEQQRMRDWLPLWKRAERRNNLVPQKSPEPVTVKQMQSTTGGATGSRSGGGGSG 60

Query: 61  GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF 120
           GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF
Sbjct: 61  GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSSDLDSPADQALFEPNPSAGF 120

Query: 121 QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE 180
           QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE
Sbjct: 121 QRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRGREVDAQNFPPKFSPQLKRE 180

Query: 181 GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM 240
           GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM
Sbjct: 181 GSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNM 240

Query: 241 DK 243
           DK
Sbjct: 241 DK 242

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BLAST of Carg28184 vs. NCBI nr
Match: XP_022925421.1 (transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription factor bHLH81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-103
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 101
           MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS
Sbjct: 1   MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 60

Query: 102 DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 161
           DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG
Sbjct: 61  DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 120

Query: 162 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 221
           REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV
Sbjct: 121 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 180

Query: 222 RRTRISDRIRKLQEVVPNMDK 243
           RRTRISDRIRKLQEVVPNMDK
Sbjct: 181 RRTRISDRIRKLQEVVPNMDK 201

[Close]

BLAST of Carg28184 vs. NCBI nr
Match: XP_022973990.1 (transcription factor bHLH81 [Cucurbita maxima])

HSP 1 Score: 374.0 bits (959), Expect = 9.8e-100
Identity = 197/201 (98.01%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 101
           MQSTTGGATGSRS    SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS
Sbjct: 1   MQSTTGGATGSRS----SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 60

Query: 102 DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 161
           DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG
Sbjct: 61  DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 120

Query: 162 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 221
           REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV
Sbjct: 121 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 180

Query: 222 RRTRISDRIRKLQEVVPNMDK 243
           RRTRISDRIRKLQEVVPNMDK
Sbjct: 181 RRTRISDRIRKLQEVVPNMDK 197

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BLAST of Carg28184 vs. NCBI nr
Match: XP_038891266.1 (transcription factor bHLH80-like [Benincasa hispida])

HSP 1 Score: 339.7 bits (870), Expect = 2.0e-89
Identity = 177/202 (87.62%), Postives = 186/202 (92.08%), Query Frame = 0


Query: 42  MQSTTG-GATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNS 101
           MQSTTG GATGSRS GGG G GLARFRSAPAAWLEALLED+EED L+P+ CLTQLLA NS
Sbjct: 1   MQSTTGAGATGSRSSGGGGGAGLARFRSAPAAWLEALLEDDEEDPLKPSHCLTQLLAANS 60

Query: 102 SDLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKR 161
           SDLDSPADQ LF+PNPS  F RQNSSPAEFL ASGIGEGFY++YA+NSS  L++SPTSKR
Sbjct: 61  SDLDSPADQPLFDPNPSPAFHRQNSSPAEFLAASGIGEGFYTAYALNSSPTLDVSPTSKR 120

Query: 162 GREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAER 221
            REVDAQNF PKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAER
Sbjct: 121 AREVDAQNFSPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAER 180

Query: 222 VRRTRISDRIRKLQEVVPNMDK 243
           VRRTRISDRIRKLQEVVPNMDK
Sbjct: 181 VRRTRISDRIRKLQEVVPNMDK 202

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BLAST of Carg28184 vs. ExPASy Swiss-Prot
Match: Q9C8P8 (Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.8e-51
Identity = 123/225 (54.67%), Postives = 156/225 (69.33%), Query Frame = 0


Query: 42  MQST--TGGATGSRSGGGG--SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 101
           MQST  +GG++G   GGGG  S  GL+R RSAPA W+E LLE++EE+ L+PN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 102 GN------------SSDLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIG-EGFYSSY 161
           GN            S +  S  +Q L+  +   GF RQNSSPA+FL  SG G +G++S++
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLYNHHQGGGFHRQNSSPADFLSGSGSGTDGYFSNF 120

Query: 162 AINS-----SAALEISPTSKRGREVDAQNFPPKFSPQLKRE--GSGVSSLIDMEMEKLLE 221
            I +     S  ++ISPT KR R+++ Q     FS QLK E    G+S ++DM M+K+ E
Sbjct: 121 GIPANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNMDKIFE 180

Query: 222 DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 243
           DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIR+LQE+VPNMDK
Sbjct: 181 DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDK 219

[Close]

BLAST of Carg28184 vs. ExPASy Swiss-Prot
Match: Q9M0R0 (Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 8.7e-51
Identity = 124/228 (54.39%), Postives = 153/228 (67.11%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGG-------GLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQ 101
           MQ T+ G++G    GGG GG       GL+R RSAPA WLEALLE++EE+ L+PN  LT 
Sbjct: 1   MQPTSVGSSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTD 60

Query: 102 LLAGNSSDLDS---------PADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYA 161
           LL GNS+DL +         P +Q L++     GF RQNS+PA+FL  S   +GF  S+ 
Sbjct: 61  LLTGNSNDLPTSRGSFEFPIPVEQGLYQ---QGGFHRQNSTPADFLSGS---DGFIQSFG 120

Query: 162 INS-----SAALEISPTSKRGREVDAQNFPPKFSPQLKREGS------GVSSLIDMEMEK 221
           I +     S  +++SP SKR RE++A    P+F+ Q+K E S      GVSS+ DM ME 
Sbjct: 121 IQANYDYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMEN 180

Query: 222 LLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 243
           L+EDSV  RVRAKRGCATHPRSIAERVRRTRISDRIRKLQE+VPNMDK
Sbjct: 181 LMEDSVAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDK 222

[Close]

BLAST of Carg28184 vs. ExPASy Swiss-Prot
Match: Q66GR3 (Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.4e-18
Identity = 44/66 (66.67%), Postives = 55/66 (83.33%), Query Frame = 0


Query: 177 LKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEV 236
           L +  S  S ++ ++    L+DSVPC++RAKRGCATHPRSIAERVRRTRIS+R+RKLQE+
Sbjct: 252 LPKSSSTASDMVSVDKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQEL 311

Query: 237 VPNMDK 243
           VPNMDK
Sbjct: 312 VPNMDK 317

[Close]

BLAST of Carg28184 vs. ExPASy Swiss-Prot
Match: Q9C690 (Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 8.3e-17
Identity = 45/67 (67.16%), Postives = 55/67 (82.09%), Query Frame = 0


Query: 175 PQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQ 234
           P L    S   SL D  +E+LL DS+PC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQ
Sbjct: 277 PPLAHHMSLPKSLSD--IEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQ 336

Query: 235 EVVPNMD 242
           ++VPNMD
Sbjct: 337 DLVPNMD 341

[Close]

BLAST of Carg28184 vs. ExPASy Swiss-Prot
Match: Q8H102 (Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 9.1e-16
Identity = 74/199 (37.19%), Postives = 96/199 (48.24%), Query Frame = 0


Query: 55  GGGGSGG--GLARFRSAPAAWLEALLEDEEE-DLLRPNQCLTQLLAGNSSDLDSPADQAL 114
           GGG S G   LAR RS+PA +   L  D+    L +P    +     N     S     L
Sbjct: 129 GGGNSSGSYSLARQRSSPADFFTYLASDKNNFSLNQPTSDYSPQGGSNGGRGHSRLKSQL 188

Query: 115 FEPNPS--AGFQRQNSSP-----AEFLGASGIGEGFYSSYAINS-SAALEISPTSKRGRE 174
              N    A     N +P          A+  G     S+   S S    ++  SKR ++
Sbjct: 189 SFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSIGFTVTRPSKRSKD 248

Query: 175 VDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRR 234
           +D+  F     P      S  S        +L EDSVPC++RAKRGCATHPRSIAER RR
Sbjct: 249 MDSGLFSQYSLP------SDTSMNYMDNFMQLPEDSVPCKIRAKRGCATHPRSIAERERR 308

Query: 235 TRISDRIRKLQEVVPNMDK 243
           TRIS +++KLQ++VPNMDK
Sbjct: 309 TRISGKLKKLQDLVPNMDK 321

[Close]

BLAST of Carg28184 vs. ExPASy TrEMBL
Match: A0A6J1EC61 (transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.1e-104
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 101
           MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS
Sbjct: 1   MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 60

Query: 102 DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 161
           DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG
Sbjct: 61  DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 120

Query: 162 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 221
           REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV
Sbjct: 121 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 180

Query: 222 RRTRISDRIRKLQEVVPNMDK 243
           RRTRISDRIRKLQEVVPNMDK
Sbjct: 181 RRTRISDRIRKLQEVVPNMDK 201

[Close]

BLAST of Carg28184 vs. ExPASy TrEMBL
Match: A0A6J1ICS6 (transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 4.7e-100
Identity = 197/201 (98.01%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 101
           MQSTTGGATGSRS    SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS
Sbjct: 1   MQSTTGGATGSRS----SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGNSS 60

Query: 102 DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 161
           DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG
Sbjct: 61  DLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISPTSKRG 120

Query: 162 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 221
           REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV
Sbjct: 121 REVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERV 180

Query: 222 RRTRISDRIRKLQEVVPNMDK 243
           RRTRISDRIRKLQEVVPNMDK
Sbjct: 181 RRTRISDRIRKLQEVVPNMDK 197

[Close]

BLAST of Carg28184 vs. ExPASy TrEMBL
Match: A0A5D3BMS8 (Transcription factor bHLH80 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00070 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.9e-81
Identity = 169/209 (80.86%), Postives = 177/209 (84.69%), Query Frame = 0


Query: 42  MQSTTGGA----TGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 101
           MQSTTG A      SR+ GGG   GLARFRSAPAAWLEALLED+EED L+PN CLTQLLA
Sbjct: 1   MQSTTGAAATATASSRTSGGGP--GLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLA 60

Query: 102 GNSSDLDS-PADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISP 161
            NSSDLDS P D  LF+PNPS  F RQNSSP EFL  SGI EGFY+SY +NSS  L+ISP
Sbjct: 61  ANSSDLDSAPGDHPLFDPNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNSSPTLDISP 120

Query: 162 TSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS 221
           TSK   +VDAQNF PKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS
Sbjct: 121 TSKPATDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS 180

Query: 222 IAERVRRTRISDRIRKLQEVVPNMDKFLP 246
           IAERVRRTRISDRIRKLQEVVPNMDKFLP
Sbjct: 181 IAERVRRTRISDRIRKLQEVVPNMDKFLP 207

[Close]

BLAST of Carg28184 vs. ExPASy TrEMBL
Match: A0A0A0KY01 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652220 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 7.9e-79
Identity = 166/208 (79.81%), Postives = 176/208 (84.62%), Query Frame = 0


Query: 42  MQSTTGGAT------GSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQL 101
           MQSTTG A+       SR+  GG+  GLARFRSAPAAWLEALLED+EED L+PN CLTQL
Sbjct: 1   MQSTTGAASTATATASSRTSAGGA--GLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQL 60

Query: 102 LAGNSSDLDS-PADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEI 161
           LA NSSDLDS PAD  LF+PNPS  F RQNSSP EFL  SGI EGFY+SY +NSS  L+I
Sbjct: 61  LAANSSDLDSAPADHPLFDPNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNSSPTLDI 120

Query: 162 SPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHP 221
           SPTSK   +VDAQNF PKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHP
Sbjct: 121 SPTSKPSTDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHP 180

Query: 222 RSIAERVRRTRISDRIRKLQEVVPNMDK 243
           RSIAERVRRTRISDRIRKLQEVVPNMDK
Sbjct: 181 RSIAERVRRTRISDRIRKLQEVVPNMDK 206

[Close]

BLAST of Carg28184 vs. ExPASy TrEMBL
Match: A0A1S3CBY9 (transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 6.7e-78
Identity = 164/206 (79.61%), Postives = 173/206 (83.98%), Query Frame = 0


Query: 42  MQSTTGGA----TGSRSGGGGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 101
           MQSTTG A      SR+ GGG   GLARFRSAPAAWLEALLED+EED L+PN CLTQLLA
Sbjct: 1   MQSTTGAAATATASSRTSGGGP--GLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLA 60

Query: 102 GNSSDL-DSPADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINSSAALEISP 161
            NSSDL  +P D  LF+PNPS  F RQNSSP EFL  SGI EGFY+SY +NSS  L+ISP
Sbjct: 61  ANSSDLVSAPGDHPLFDPNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNSSPTLDISP 120

Query: 162 TSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS 221
           TSK   +VDAQNF PKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS
Sbjct: 121 TSKPATDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRS 180

Query: 222 IAERVRRTRISDRIRKLQEVVPNMDK 243
           IAERVRRTRISDRIRKLQEVVPNMDK
Sbjct: 181 IAERVRRTRISDRIRKLQEVVPNMDK 204

[Close]

BLAST of Carg28184 vs. TAIR 10
Match: AT1G35460.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 1.3e-52
Identity = 123/225 (54.67%), Postives = 156/225 (69.33%), Query Frame = 0


Query: 42  MQST--TGGATGSRSGGGG--SGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 101
           MQST  +GG++G   GGGG  S  GL+R RSAPA W+E LLE++EE+ L+PN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 102 GN------------SSDLDSPADQALFEPNPSAGFQRQNSSPAEFLGASGIG-EGFYSSY 161
           GN            S +  S  +Q L+  +   GF RQNSSPA+FL  SG G +G++S++
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLYNHHQGGGFHRQNSSPADFLSGSGSGTDGYFSNF 120

Query: 162 AINS-----SAALEISPTSKRGREVDAQNFPPKFSPQLKRE--GSGVSSLIDMEMEKLLE 221
            I +     S  ++ISPT KR R+++ Q     FS QLK E    G+S ++DM M+K+ E
Sbjct: 121 GIPANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNMDKIFE 180

Query: 222 DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 243
           DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIR+LQE+VPNMDK
Sbjct: 181 DSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDK 219

[Close]

BLAST of Carg28184 vs. TAIR 10
Match: AT4G09180.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 6.2e-52
Identity = 124/228 (54.39%), Postives = 153/228 (67.11%), Query Frame = 0


Query: 42  MQSTTGGATGSRSGGGGSGG-------GLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQ 101
           MQ T+ G++G    GGG GG       GL+R RSAPA WLEALLE++EE+ L+PN  LT 
Sbjct: 1   MQPTSVGSSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTD 60

Query: 102 LLAGNSSDLDS---------PADQALFEPNPSAGFQRQNSSPAEFLGASGIGEGFYSSYA 161
           LL GNS+DL +         P +Q L++     GF RQNS+PA+FL  S   +GF  S+ 
Sbjct: 61  LLTGNSNDLPTSRGSFEFPIPVEQGLYQ---QGGFHRQNSTPADFLSGS---DGFIQSFG 120

Query: 162 INS-----SAALEISPTSKRGREVDAQNFPPKFSPQLKREGS------GVSSLIDMEMEK 221
           I +     S  +++SP SKR RE++A    P+F+ Q+K E S      GVSS+ DM ME 
Sbjct: 121 IQANYDYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMEN 180

Query: 222 LLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 243
           L+EDSV  RVRAKRGCATHPRSIAERVRRTRISDRIRKLQE+VPNMDK
Sbjct: 181 LMEDSVAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDK 222

[Close]

BLAST of Carg28184 vs. TAIR 10
Match: AT2G42280.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 2.4e-19
Identity = 44/66 (66.67%), Postives = 55/66 (83.33%), Query Frame = 0


Query: 177 LKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQEV 236
           L +  S  S ++ ++    L+DSVPC++RAKRGCATHPRSIAERVRRTRIS+R+RKLQE+
Sbjct: 252 LPKSSSTASDMVSVDKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQEL 311

Query: 237 VPNMDK 243
           VPNMDK
Sbjct: 312 VPNMDK 317

[Close]

BLAST of Carg28184 vs. TAIR 10
Match: AT1G51140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 5.9e-18
Identity = 45/67 (67.16%), Postives = 55/67 (82.09%), Query Frame = 0


Query: 175 PQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRKLQ 234
           P L    S   SL D  +E+LL DS+PC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQ
Sbjct: 277 PPLAHHMSLPKSLSD--IEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQ 336

Query: 235 EVVPNMD 242
           ++VPNMD
Sbjct: 337 DLVPNMD 341

[Close]

BLAST of Carg28184 vs. TAIR 10
Match: AT1G05805.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 6.5e-17
Identity = 74/199 (37.19%), Postives = 96/199 (48.24%), Query Frame = 0


Query: 55  GGGGSGG--GLARFRSAPAAWLEALLEDEEE-DLLRPNQCLTQLLAGNSSDLDSPADQAL 114
           GGG S G   LAR RS+PA +   L  D+    L +P    +     N     S     L
Sbjct: 129 GGGNSSGSYSLARQRSSPADFFTYLASDKNNFSLNQPTSDYSPQGGSNGGRGHSRLKSQL 188

Query: 115 FEPNPS--AGFQRQNSSP-----AEFLGASGIGEGFYSSYAINS-SAALEISPTSKRGRE 174
              N    A     N +P          A+  G     S+   S S    ++  SKR ++
Sbjct: 189 SFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSIGFTVTRPSKRSKD 248

Query: 175 VDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRR 234
           +D+  F     P      S  S        +L EDSVPC++RAKRGCATHPRSIAER RR
Sbjct: 249 MDSGLFSQYSLP------SDTSMNYMDNFMQLPEDSVPCKIRAKRGCATHPRSIAERERR 308

Query: 235 TRISDRIRKLQEVVPNMDK 243
           TRIS +++KLQ++VPNMDK
Sbjct: 309 TRISGKLKKLQDLVPNMDK 321

The following BLAST results are available for this feature:

BLAST of Carg28184 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025210.1	4.8e-131	100.00	Transcription factor bHLH80, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6592393.1	5.9e-129	100.00	Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma ...	[more]
XP_022925421.1	1.5e-103	100.00	transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription f...	[more]
XP_022973990.1	9.8e-100	98.01	transcription factor bHLH81 [Cucurbita maxima]	[more]
XP_038891266.1	2.0e-89	87.62	transcription factor bHLH80-like [Benincasa hispida]	[more]

BLAST of Carg28184 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8P8	1.8e-51	54.67	Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1	[more]
Q9M0R0	8.7e-51	54.39	Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1	[more]
Q66GR3	3.4e-18	66.67	Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=...	[more]
Q9C690	8.3e-17	67.16	Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=...	[more]
Q8H102	9.1e-16	37.19	Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=...	[more]

BLAST of Carg28184 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EC61	7.1e-104	100.00	transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 S...	[more]
A0A6J1ICS6	4.7e-100	98.01	transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=...	[more]
A0A5D3BMS8	4.9e-81	80.86	Transcription factor bHLH80 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KY01	7.9e-79	79.81	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652220 PE=4 S...	[more]
A0A1S3CBY9	6.7e-78	79.61	transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 P...	[more]

BLAST of Carg28184 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35460.1	1.3e-52	54.67	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G09180.1	6.2e-52	54.39	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G42280.1	2.4e-19	66.67	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G51140.1	5.9e-18	67.16	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G05805.1	6.5e-17	37.19	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..53
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 26..62
None	No IPR available	PANTHER	PTHR16223	TRANSCRIPTION FACTOR BHLH83-RELATED	coord: 55..242
None	No IPR available	PANTHER	PTHR16223:SF225	TRANSCRIPTION FACTOR BHLH80	coord: 55..242
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 210..245 score: 9.184597
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 213..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28184-RA	Carg28184-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding