Carg28172 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg28172
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCarg_Chr09: 10176064 .. 10177891 (+)
RNA-Seq ExpressionCarg28172
SyntenyCarg28172
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATGCCTAGACTTGAAAGAAGCAATCTCGAGTGTGTTGTTTTTGCTGCTCCCAAGGTGTGCTGATCTTACAGAGCTTCTCCAGGTTCAGTTGCTTTTTGGCTGTTAAATATGGGAAGGAATTTGTATCAGCTGCAACAGAGCTTATGCCTAAGCTGTGGCGTTAATCGTCAGGTTCGTAAAATTTCTTGTTTACGTTTGATTAGTCACAATCGCACTTTGATGGCAGCAGACTTGCGATTGTGCGGCACTCACTTCTCAGCAACAAGTGAGTTAAGCCTATTCGTTCTTGTGTCGCTTACGGCCAAGCGACGTATGCTCGTTCCTCTGGTCCCGGTTCAAGAAGAAAGTTTTATAAAATGGGGGCAGAGAGATTTCAAAAGAGAGTTTTGAAAGCAAGAATTGAGAAATTGAAATTGGTGTTATATGAGAATGTAAGCAAAAGACAACAACAACGGCTTACTGCATAAAACTATTGGGTGGAGGGATAGAAGGAAAGCGCTTTGCTGCAGTCTATAGATTTATATATTTCCTCTTAATTTTGATTGCAGCTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCCGAGAAGAAACTGAAGCTCTTAAAGGAAATCGCCGAAGAACACGACGTAGATTGGGATCCTGCTGGAACTGAAGCCGAGTTTAACAAATCACCAGAAGATTTGCTTGTAAGTGTTGCTTCTTTTTGTTTGTCTCAACTTGTGGTGACTCTTTATGCCTTAGTTTCTGCTCTTTCTTTGCAGAATGGATCGGCACAGTTCGTCAGCGCATCAAAATTACCCCTTCCTCAAGAGAAACACAACGAGGCATACAATGCACCACAACCTGATTCTGATTCTGAATTGGACATGTTAGACTTCCCTGAAGTTCCAAAGATATCAGTTTCTCCACATTCTACTATAGATCCTGGTTCTGCTTCATCGATGATCCCTCCTCCGTCAACGTCGCCACCGCCTGAAACTACTCATGATTCATCGAAGTATTCTTGTATTCCTAAAACCCCACCAGAAGATTTACAATTGAGACGTGGGGAGGTGACACCGGTACGATCTGTTTCACCCAGCAAGGATCAAATGAATATTTCGGTCGGGGAAGATAAACAGTTTTTGCCTTTTATAGCTCCCCCATCAAGGACCCCTGAGGAGAAGTTTAGTTTCAAACCACAGTTCGAACCCGAGATAAATTCACCACCACCCTCGGTTTCAAGGACACCTGATGAGAAATTTGGTTTGAAGTATGAACAGGAGATAATTTCACCCCCACCCTCTGGTTCAAGGACAAAGAGTGATGTGAGTTTGAATTTGCAGGACGTGTTGGCAGCTGCTCAGGCTGCTGTTGAAACCGCCGAGCGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTTTAACAAAGGTTAGAATCGACACGCTCACAATGAAAACGAACGATCAGGACCCTGAAATTTGTTGCGAGAATCCGTTCCATGGAGCTACTACAAGTCCACCAGATCATGAACAAACATTCCACCTAAACCAACAAGATTCTATAGGGCATCATTCTAGCTCTGTGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAAGAAAGCCCTGGTTCAGATGTTTCCGACCACCACCGGTTTGAGTCACAGAAATCGGGTTTCGACTCCTCTCCCGGTGATTCACCATCCGAACACATAACAATCCCCCATCAGCCCCAGAGGCGTCCATCGATGGACGACGACCCATACTTTTCGTACCCAAATTTATTTACATCACAGAAACCAAATCCAGGATTTGATCATTCATCAGCTGATGGTTCT

mRNA sequence

GAATGCCTAGACTTGAAAGAAGCAATCTCGAGTGTGTTGTTTTTGCTGCTCCCAAGGTGTGCTGATCTTACAGAGCTTCTCCAGGTTCGTAAAATTTCTTGTTTACGTTTGATTAGTCACAATCGCACTTTGATGGCAGCAGACTTGCGATTGTGCGGCACTCACTTCTCAGCAACAAGTGAGTTAAGCCTATTCGTTCTTGTGTCGCTTACGGCCAAGCGACGTATGCTCGTTCCTCTGGTCCCGCTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCCGAGAAGAAACTGAAGCTCTTAAAGGAAATCGCCGAAGAACACGACGTAGATTGGGATCCTGCTGGAACTGAAGCCGAGTTTAACAAATCACCAGAAGATTTGCTTAATGGATCGGCACAGTTCGTCAGCGCATCAAAATTACCCCTTCCTCAAGAGAAACACAACGAGGCATACAATGCACCACAACCTGATTCTGATTCTGAATTGGACATGTTAGACTTCCCTGAAGTTCCAAAGATATCAGTTTCTCCACATTCTACTATAGATCCTGGTTCTGCTTCATCGATGATCCCTCCTCCGTCAACGTCGCCACCGCCTGAAACTACTCATGATTCATCGAAGTATTCTTGTATTCCTAAAACCCCACCAGAAGATTTACAATTGAGACGTGGGGAGGTGACACCGGTACGATCTGTTTCACCCAGCAAGGATCAAATGAATATTTCGGTCGGGGAAGATAAACAGTTTTTGCCTTTTATAGCTCCCCCATCAAGGACCCCTGAGGAGAAGTTTAGTTTCAAACCACAGTTCGAACCCGAGATAAATTCACCACCACCCTCGGTTTCAAGGACACCTGATGAGAAATTTGGTTTGAAGTATGAACAGGAGATAATTTCACCCCCACCCTCTGGTTCAAGGACAAAGAGTGATGTGAGTTTGAATTTGCAGGACGTGTTGGCAGCTGCTCAGGCTGCTGTTGAAACCGCCGAGCGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTTTAACAAAGGTTAGAATCGACACGCTCACAATGAAAACGAACGATCAGGACCCTGAAATTTGTTGCGAGAATCCGTTCCATGGAGCTACTACAAGTCCACCAGATCATGAACAAACATTCCACCTAAACCAACAAGATTCTATAGGGCATCATTCTAGCTCTGTGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAAGAAAGCCCTGGTTCAGATGTTTCCGACCACCACCGGTTTGAGTCACAGAAATCGGGTTTCGACTCCTCTCCCGGTGATTCACCATCCGAACACATAACAATCCCCCATCAGCCCCAGAGGCGTCCATCGATGGACGACGACCCATACTTTTCGTACCCAAATTTATTTACATCACAGAAACCAAATCCAGGATTTGATCATTCATCAGCTGATGGTTCT

Coding sequence (CDS)

GAATGCCTAGACTTGAAAGAAGCAATCTCGAGTGTGTTGTTTTTGCTGCTCCCAAGGTGTGCTGATCTTACAGAGCTTCTCCAGGTTCGTAAAATTTCTTGTTTACGTTTGATTAGTCACAATCGCACTTTGATGGCAGCAGACTTGCGATTGTGCGGCACTCACTTCTCAGCAACAAGTGAGTTAAGCCTATTCGTTCTTGTGTCGCTTACGGCCAAGCGACGTATGCTCGTTCCTCTGGTCCCGCTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCCGAGAAGAAACTGAAGCTCTTAAAGGAAATCGCCGAAGAACACGACGTAGATTGGGATCCTGCTGGAACTGAAGCCGAGTTTAACAAATCACCAGAAGATTTGCTTAATGGATCGGCACAGTTCGTCAGCGCATCAAAATTACCCCTTCCTCAAGAGAAACACAACGAGGCATACAATGCACCACAACCTGATTCTGATTCTGAATTGGACATGTTAGACTTCCCTGAAGTTCCAAAGATATCAGTTTCTCCACATTCTACTATAGATCCTGGTTCTGCTTCATCGATGATCCCTCCTCCGTCAACGTCGCCACCGCCTGAAACTACTCATGATTCATCGAAGTATTCTTGTATTCCTAAAACCCCACCAGAAGATTTACAATTGAGACGTGGGGAGGTGACACCGGTACGATCTGTTTCACCCAGCAAGGATCAAATGAATATTTCGGTCGGGGAAGATAAACAGTTTTTGCCTTTTATAGCTCCCCCATCAAGGACCCCTGAGGAGAAGTTTAGTTTCAAACCACAGTTCGAACCCGAGATAAATTCACCACCACCCTCGGTTTCAAGGACACCTGATGAGAAATTTGGTTTGAAGTATGAACAGGAGATAATTTCACCCCCACCCTCTGGTTCAAGGACAAAGAGTGATGTGAGTTTGAATTTGCAGGACGTGTTGGCAGCTGCTCAGGCTGCTGTTGAAACCGCCGAGCGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTTTAACAAAGGTTAGAATCGACACGCTCACAATGAAAACGAACGATCAGGACCCTGAAATTTGTTGCGAGAATCCGTTCCATGGAGCTACTACAAGTCCACCAGATCATGAACAAACATTCCACCTAAACCAACAAGATTCTATAGGGCATCATTCTAGCTCTGTGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAAGAAAGCCCTGGTTCAGATGTTTCCGACCACCACCGGTTTGAGTCACAGAAATCGGGTTTCGACTCCTCTCCCGGTGATTCACCATCCGAACACATAACAATCCCCCATCAGCCCCAGAGGCGTCCATCGATGGACGACGACCCATACTTTTCGTACCCAAATTTATTTACATCACAGAAACCAAATCCAGGATTTGATCATTCATCAGCTGATGGTTCT

Protein sequence

ECLDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSPHSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKDQMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEIISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQDPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSADGS
Homology
BLAST of Carg28172 vs. NCBI nr
Match: KAG7025137.1 (hypothetical protein SDJN02_13960, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 935.6 bits (2417), Expect = 1.7e-268
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   ECLDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATS 60
           ECLDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATS
Sbjct: 1   ECLDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATS 60

Query: 61  ELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAE 120
           ELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAE
Sbjct: 61  ELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAE 120

Query: 121 FNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSPHS 180
           FNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSPHS
Sbjct: 121 FNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSPHS 180

Query: 181 TIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKDQM 240
           TIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKDQM
Sbjct: 181 TIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKDQM 240

Query: 241 NISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEIIS 300
           NISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEIIS
Sbjct: 241 NISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEIIS 300

Query: 301 PPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQDP 360
           PPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQDP
Sbjct: 301 PPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQDP 360

Query: 361 EICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDH 420
           EICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDH
Sbjct: 361 EICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDH 420

Query: 421 HRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSA 480
           HRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSA
Sbjct: 421 HRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSA 480

Query: 481 DGS 484
           DGS
Sbjct: 481 DGS 483

BLAST of Carg28172 vs. NCBI nr
Match: KAG6592298.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 795.4 bits (2053), Expect = 2.7e-226
Identity = 429/486 (88.27%), Postives = 439/486 (90.33%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AEFNKSPEDLLNGSA+FVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP
Sbjct: 217 AEFNKSPEDLLNGSARFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
           QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI
Sbjct: 337 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG-HHSSSVPYYSQDSFQEHHKESPGSDV 420
           DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG HHSSS+PYYSQDSFQEHHKESPGSDV
Sbjct: 457 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHSSSMPYYSQDSFQEHHKESPGSDV 516

Query: 421 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDH 480
           SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSMDDDPYFSYPNLFTSQ PNPGFDH
Sbjct: 517 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDPYFSYPNLFTSQNPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. NCBI nr
Match: XP_022930138.1 (uncharacterized protein LOC111436655 isoform X1 [Cucurbita moschata] >XP_022930139.1 uncharacterized protein LOC111436655 isoform X2 [Cucurbita moschata] >XP_022930140.1 uncharacterized protein LOC111436655 isoform X1 [Cucurbita moschata])

HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 426/486 (87.65%), Postives = 438/486 (90.12%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       L+ELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LVELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP
Sbjct: 217 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIP+TPPEDLQLRRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPETPPEDLQLRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
           QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI
Sbjct: 337 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPS SRTKSDVSL+LQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSASRTKSDVSLDLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG-HHSSSVPYYSQDSFQEHHKESPGSDV 420
            PEICCENPFHGATTSPPDHEQTFHLNQQDSIG HHSSS+PYYSQDSFQEHHKESPGSDV
Sbjct: 457 GPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHSSSMPYYSQDSFQEHHKESPGSDV 516

Query: 421 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDH 480
           SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSMDDDPYFSYPNLFTSQKPNPGFDH
Sbjct: 517 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. NCBI nr
Match: XP_023536600.1 (uncharacterized protein LOC111797723 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.8 bits (2028), Expect = 2.1e-223
Identity = 425/486 (87.45%), Postives = 437/486 (89.92%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP
Sbjct: 217 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPETT DSSKYSCIP+TPPEDL LRRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETTRDSSKYSCIPETPPEDLHLRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
           QMNISVGEDKQFLPFIAPPSRT EEKFSFKPQFEPEINSPPPSVSRTPDEK+GLKYEQEI
Sbjct: 337 QMNISVGEDKQFLPFIAPPSRTREEKFSFKPQFEPEINSPPPSVSRTPDEKYGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPSGSRTKSDVSL+LQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSGSRTKSDVSLDLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG-HHSSSVPYYSQDSFQEHHKESPGSDV 420
           DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG HHSSS+PYYSQDSFQEHHKESPGSDV
Sbjct: 457 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHSSSMPYYSQDSFQEHHKESPGSDV 516

Query: 421 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDH 480
           SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSMDDDPYFSYPNLFTSQKPNPGFDH
Sbjct: 517 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. NCBI nr
Match: XP_022974062.1 (uncharacterized protein LOC111472688 [Cucurbita maxima] >XP_022974064.1 uncharacterized protein LOC111472688 [Cucurbita maxima])

HSP 1 Score: 753.8 bits (1945), Expect = 9.0e-214
Identity = 409/486 (84.16%), Postives = 425/486 (87.45%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDP GTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPVGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AE+NKSPEDLLNGSAQFVS SKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKI+VSP
Sbjct: 217 AEYNKSPEDLLNGSAQFVSPSKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKITVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPET HDS   SC P+TPP+DL +RRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETKHDSLTNSCSPETPPQDLHMRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
            MNISVGEDKQFLPFIAPPSRT EEKFSFKPQFEPEINSPPPSVSRTPDEK+GLKYEQEI
Sbjct: 337 LMNISVGEDKQFLPFIAPPSRTLEEKFSFKPQFEPEINSPPPSVSRTPDEKYGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPSGSRTKSDVSL+LQDVL AAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSGSRTKSDVSLDLQDVLTAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVS 420
            PEICCENPFHGAT S PDHEQTFHLNQQDSIGHHSSS+PYYSQDSF EHHKESPGSDVS
Sbjct: 457 GPEICCENPFHGATASTPDHEQTFHLNQQDSIGHHSSSMPYYSQDSFPEHHKESPGSDVS 516

Query: 421 DHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSM-DDDPYFSYPNLFTSQKPNPGFDH 480
           DHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSM DDDPYFSYPNLFTSQKPNPGFDH
Sbjct: 517 DHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDDPYFSYPNLFTSQKPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. ExPASy TrEMBL
Match: A0A6J1EU69 (uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436655 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.5e-225
Identity = 426/486 (87.65%), Postives = 438/486 (90.12%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       L+ELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LVELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP
Sbjct: 217 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIP+TPPEDLQLRRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPETPPEDLQLRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
           QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI
Sbjct: 337 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPS SRTKSDVSL+LQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSASRTKSDVSLDLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIG-HHSSSVPYYSQDSFQEHHKESPGSDV 420
            PEICCENPFHGATTSPPDHEQTFHLNQQDSIG HHSSS+PYYSQDSFQEHHKESPGSDV
Sbjct: 457 GPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHSSSMPYYSQDSFQEHHKESPGSDV 516

Query: 421 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSMDDDPYFSYPNLFTSQKPNPGFDH 480
           SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSMDDDPYFSYPNLFTSQKPNPGFDH
Sbjct: 517 SDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDPYFSYPNLFTSQKPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. ExPASy TrEMBL
Match: A0A6J1I985 (uncharacterized protein LOC111472688 OS=Cucurbita maxima OX=3661 GN=LOC111472688 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.3e-214
Identity = 409/486 (84.16%), Postives = 425/486 (87.45%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTELLQV+ +  ++                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELLQVQLLFAVKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDP GTE
Sbjct: 157 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPVGTE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKISVSP 180
           AE+NKSPEDLLNGSAQFVS SKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKI+VSP
Sbjct: 217 AEYNKSPEDLLNGSAQFVSPSKLPLPQEKHNEAYNAPQPDSDSELDMLDFPEVPKITVSP 276

Query: 181 HSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRSVSPSKD 240
           HSTIDPGSASSMIPPPSTSPPPET HDS   SC P+TPP+DL +RRGEVTPVRSVSPSKD
Sbjct: 277 HSTIDPGSASSMIPPPSTSPPPETKHDSLTNSCSPETPPQDLHMRRGEVTPVRSVSPSKD 336

Query: 241 QMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDEKFGLKYEQEI 300
            MNISVGEDKQFLPFIAPPSRT EEKFSFKPQFEPEINSPPPSVSRTPDEK+GLKYEQEI
Sbjct: 337 LMNISVGEDKQFLPFIAPPSRTLEEKFSFKPQFEPEINSPPPSVSRTPDEKYGLKYEQEI 396

Query: 301 ISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 360
           ISPPPSGSRTKSDVSL+LQDVL AAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ
Sbjct: 397 ISPPPSGSRTKSDVSLDLQDVLTAAQAAVETAERAAAAARSAASLTKVRIDTLTMKTNDQ 456

Query: 361 DPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHSSSVPYYSQDSFQEHHKESPGSDVS 420
            PEICCENPFHGAT S PDHEQTFHLNQQDSIGHHSSS+PYYSQDSF EHHKESPGSDVS
Sbjct: 457 GPEICCENPFHGATASTPDHEQTFHLNQQDSIGHHSSSMPYYSQDSFPEHHKESPGSDVS 516

Query: 421 DHHRFESQKSGFDSSPGDSPSEHITIPHQPQRRPSM-DDDPYFSYPNLFTSQKPNPGFDH 480
           DHHRFESQKSGFDSSPGDSPSEHITIPHQPQR PSM DDDPYFSYPNLFTSQKPNPGFDH
Sbjct: 517 DHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSMDDDDPYFSYPNLFTSQKPNPGFDH 555

Query: 481 SSADGS 484
           SSADGS
Sbjct: 577 SSADGS 555

BLAST of Carg28172 vs. ExPASy TrEMBL
Match: A0A0A0KBZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.4e-159
Identity = 338/516 (65.50%), Postives = 374/516 (72.48%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSAT 60
           EC LDLKEAISSV F   PRCADLTEL+QV+              M    +      SA 
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELIQVQ--------------MLFGAKYGKEFLSAA 156

Query: 61  SELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEA 120
           +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHD+DW+PA TEA
Sbjct: 157 TEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETEA 216

Query: 121 EFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYN-------APQPDSDSELDMLDFPEVP 180
           EFNKSPEDLLNGS QFV ASKLPLPQEKHNE  N        PQPDSDSELDMLDFPEVP
Sbjct: 217 EFNKSPEDLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEVP 276

Query: 181 KISVSPHSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVRS 240
           K+SV P  TID GSA SMIPPPS SPP ET H S +YS IP++PP++L  R  EVT VRS
Sbjct: 277 KMSVCPQPTIDAGSAPSMIPPPSVSPPRETDHASFEYSGIPESPPQNLHSRHEEVTLVRS 336

Query: 241 VSPSKDQMNISVGEDKQFLPFIAPPS------------------RTPEEKFSFKPQFEPE 300
           VSPS DQMN+SVGEDKQFLPFI PPS                   TPEEKF  KP+   E
Sbjct: 337 VSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEKFDIKPRIREE 396

Query: 301 INSPPPSVSRTPDEKFGLK--YEQEIISPPPSGSRTKS----DVSLNLQDVLAAAQAAVE 360
           INSP PSVSR  +EK+G K  +E EI S P S SRT S    D+ ++LQDVLAAAQAA E
Sbjct: 397 INSPLPSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQDVLAAAQAAAE 456

Query: 361 TAERAAAAARSAASLTKVRIDTLTMKTNDQDPEICCENPFHGATTSPPDHEQTFHLNQQD 420
           TAERAAAAARSAASL KVRID LT K ND+DPEI CENPFHG T SP DH +T+ LNQQD
Sbjct: 457 TAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADHTETYKLNQQD 516

Query: 421 SIGHHSSSVPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITI-PHQ 480
           S+G+HSS++PYYSQDSFQEHHK S   +VSDH + + +KS FD SPG+SPSEH T+ PHQ
Sbjct: 517 SLGYHSSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSPSEHQTVAPHQ 576

Query: 481 PQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSADGS 484
           PQR PSMDDDPYFSYPNLFTSQKPNPGFDHSSA GS
Sbjct: 577 PQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 586

BLAST of Carg28172 vs. ExPASy TrEMBL
Match: A0A5D3D4I0 (IST1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold607G00160 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.4e-156
Identity = 338/517 (65.38%), Postives = 373/517 (72.15%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTEL+QV+ +   +                G  F SA
Sbjct: 390 ECPLDLKEAISSVCF-AAPRCADLTELIQVQMLFGAKY---------------GKEFVSA 449

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHD+DW+PA TE
Sbjct: 450 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETE 509

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYN-------APQPDSDSELDMLDFPEV 180
           AEFNKSPEDLLNG   FV  SKLPLPQEKHNE  N        PQPDSDSELDMLDFPEV
Sbjct: 510 AEFNKSPEDLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEV 569

Query: 181 PKISVSPHSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVR 240
           PK+SV P  TID GSA SMI PPS SP PET   S KYS IP++PP++L  R GEVT VR
Sbjct: 570 PKMSVCPQPTIDAGSAPSMILPPSVSPQPETDRASFKYSGIPESPPQNLHSRHGEVTLVR 629

Query: 241 SVSPSKDQMNISVGEDKQFLPFIAPPS------------------RTPEEKFSFKPQFEP 300
           SVSPS DQMN+SVGEDKQFLPFI PPS                   TPEEKF  KP+   
Sbjct: 630 SVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTPEEKFDIKPRITE 689

Query: 301 EINSPPPSVSRTPDEKFGLK--YEQEIISPPPSGSRT----KSDVSLNLQDVLAAAQAAV 360
           EINSP PSVSR  +E+FG K  ++ EI S P S SRT     +D+S++LQDVLAAAQAA 
Sbjct: 690 EINSPLPSVSRASEEQFGFKPRFDSEINSTPSSVSRTMCEVNADLSVDLQDVLAAAQAAA 749

Query: 361 ETAERAAAAARSAASLTKVRIDTLTMKTNDQDPEICCENPFHGATTSPPDHEQTFHLNQQ 420
           ETAERAAAAARSAASL KVRID LT K ND+DPEI CENPFHGAT SP  H +T+ LNQQ
Sbjct: 750 ETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASPAGHTETYKLNQQ 809

Query: 421 DSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITI-PH 480
           DS+GHHSSS+PYYSQDS QEHHK S   +VSDH + E +KS FDSSPG+SPSEH T+ PH
Sbjct: 810 DSLGHHSSSMPYYSQDSSQEHHKLSRDPEVSDHSQLEPEKSSFDSSPGNSPSEHQTVAPH 869

Query: 481 QPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSADGS 484
           QPQR PSMDDDPYFSYPNLFTSQKPNPGFDHSSA GS
Sbjct: 870 QPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 876

BLAST of Carg28172 vs. ExPASy TrEMBL
Match: A0A1S4E4M8 (uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.4e-156
Identity = 338/517 (65.38%), Postives = 373/517 (72.15%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHF-SA 60
           EC LDLKEAISSV F   PRCADLTEL+QV+ +   +                G  F SA
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCADLTELIQVQMLFGAKY---------------GKEFVSA 156

Query: 61  TSELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTE 120
            +EL    + +    R+       LIELLSVRAPSPEKKLKLLKEIAEEHD+DW+PA TE
Sbjct: 157 ATEL----MPNCGVNRQ-------LIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETE 216

Query: 121 AEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYN-------APQPDSDSELDMLDFPEV 180
           AEFNKSPEDLLNG   FV  SKLPLPQEKHNE  N        PQPDSDSELDMLDFPEV
Sbjct: 217 AEFNKSPEDLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEV 276

Query: 181 PKISVSPHSTIDPGSASSMIPPPSTSPPPETTHDSSKYSCIPKTPPEDLQLRRGEVTPVR 240
           PK+SV P  TID GSA SMI PPS SP PET   S KYS IP++PP++L  R GEVT VR
Sbjct: 277 PKMSVCPQPTIDAGSAPSMILPPSVSPQPETDRASFKYSGIPESPPQNLHSRHGEVTLVR 336

Query: 241 SVSPSKDQMNISVGEDKQFLPFIAPPS------------------RTPEEKFSFKPQFEP 300
           SVSPS DQMN+SVGEDKQFLPFI PPS                   TPEEKF  KP+   
Sbjct: 337 SVSPSNDQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTPEEKFDIKPRITE 396

Query: 301 EINSPPPSVSRTPDEKFGLK--YEQEIISPPPSGSRT----KSDVSLNLQDVLAAAQAAV 360
           EINSP PSVSR  +E+FG K  ++ EI S P S SRT     +D+S++LQDVLAAAQAA 
Sbjct: 397 EINSPLPSVSRASEEQFGFKPRFDSEINSTPSSVSRTMCEVNADLSVDLQDVLAAAQAAA 456

Query: 361 ETAERAAAAARSAASLTKVRIDTLTMKTNDQDPEICCENPFHGATTSPPDHEQTFHLNQQ 420
           ETAERAAAAARSAASL KVRID LT K ND+DPEI CENPFHGAT SP  H +T+ LNQQ
Sbjct: 457 ETAERAAAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASPAGHTETYKLNQQ 516

Query: 421 DSIGHHSSSVPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITI-PH 480
           DS+GHHSSS+PYYSQDS QEHHK S   +VSDH + E +KS FDSSPG+SPSEH T+ PH
Sbjct: 517 DSLGHHSSSMPYYSQDSSQEHHKLSRDPEVSDHSQLEPEKSSFDSSPGNSPSEHQTVAPH 576

Query: 481 QPQRRPSMDDDPYFSYPNLFTSQKPNPGFDHSSADGS 484
           QPQR PSMDDDPYFSYPNLFTSQKPNPGFDHSSA GS
Sbjct: 577 QPQRLPSMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 583

BLAST of Carg28172 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 199.1 bits (505), Expect = 7.9e-51
Identity = 219/590 (37.12%), Postives = 280/590 (47.46%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSAT 60
           EC LDLKEAISSV F   PRC+DLTEL QV +I  +          A++L+        T
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCSDLTELQQV-QILFVSKYGKEFVAAASELKPDSGVNRKT 156

Query: 61  SELSLFVLVSLTAKRRMLV------PLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWD 120
             L      SL   R + +       ++ L+ELLSVRAPSPE KLKLLKEIAEEH++DWD
Sbjct: 157 ESLIFIAWFSLVETRDLFMFLYFSNSILQLVELLSVRAPSPETKLKLLKEIAEEHELDWD 216

Query: 121 PAGTEAEFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYN-------APQPDSDSELDML 180
           PA TE +  KS EDLL+G  QF   SKLPLP+E+ NE  N         + DSDSE D+L
Sbjct: 217 PASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQ-NEKTNLTSLSAAKEKSDSDSEYDIL 276

Query: 181 DFPEVPKISVSPHSTIDPGSASSMIPPPSTSPPPE-TTHD---SSKYSCIPKTPPEDLQL 240
           DFPEVP + + P     PG+ S   P  + S   E T+HD    S+ + + KT  +    
Sbjct: 277 DFPEVPNVLLRP----TPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEKTASK---- 336

Query: 241 RRGEVTPVRSVSPSKDQMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSV 300
                   R   P+K   + +V E +Q  P +        E F  K    P I++  P  
Sbjct: 337 --------RDEHPAK--ASKTVVEGQQSSPILM-------ESFEKKNYSPPSIDAVGP-- 396

Query: 301 SRTPDEKFGLKYEQEIISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAAS 360
              P ++ G   +           R  SD   +LQDVL AAQAA ++AERAA+AARSAAS
Sbjct: 397 --IPTKESGASRDT---------PRKISDG--DLQDVLMAAQAAADSAERAASAARSAAS 456

Query: 361 LTKVRIDTLTMKTNDQDPEICCENPFHG--------------------------ATTS-- 420
           L ++RI+ LT KT+DQ PE   ENPFH                           A TS  
Sbjct: 457 LAQLRINELTRKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSSGDLTELQRAETSSL 516

Query: 421 ---------PPDHE----------------------QTFHLNQQDSIGHHSSSVPYY--- 480
                    P  H                       Q FH  ++    H +SS   Y   
Sbjct: 517 FNSEQNNQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFHSMEKPQFDHQNSSGSNYDDL 576

Query: 481 ------SQDSFQEHHKESPGSDVSD--HHRFES-QKSGFD------SSPGD--------- 484
                 S +  Q  H+ S GS+  D   HRF S +K  FD      SS GD         
Sbjct: 577 TPQRFSSLEKPQFDHQNSSGSNYDDLTPHRFPSMEKPQFDHQNSSVSSYGDLPELQRPET 636

BLAST of Carg28172 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 193.0 bits (489), Expect = 5.7e-49
Identity = 218/584 (37.33%), Postives = 276/584 (47.26%), Query Frame = 0

Query: 1   EC-LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSAT 60
           EC LDLKEAISSV F   PRC+DLTEL QV+    L +  + +  +A           A 
Sbjct: 97  ECPLDLKEAISSVCF-AAPRCSDLTELQQVQ---ILFVSKYGKEFVA-----------AA 156

Query: 61  SELSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEA 120
           SEL     V+             L+ELLSVRAPSPE KLKLLKEIAEEH++DWDPA TE 
Sbjct: 157 SELKPDSGVNR-----------KLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTET 216

Query: 121 EFNKSPEDLLNGSAQFVSASKLPLPQEKHNEAYN-------APQPDSDSELDMLDFPEVP 180
           +  KS EDLL+G  QF   SKLPLP+E+ NE  N         + DSDSE D+LDFPEVP
Sbjct: 217 DLFKSHEDLLDGPKQFGGGSKLPLPEEQ-NEKTNLTSLSAAKEKSDSDSEYDILDFPEVP 276

Query: 181 KISVSPHSTIDPGSASSMIPPPSTSPPPE-TTHD---SSKYSCIPKTPPEDLQLRRGEVT 240
            + + P     PG+ S   P  + S   E T+HD    S+ + + KT  +          
Sbjct: 277 NVLLRP----TPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEKTASK---------- 336

Query: 241 PVRSVSPSKDQMNISVGEDKQFLPFIAPPSRTPEEKFSFKPQFEPEINSPPPSVSRTPDE 300
             R   P+K   + +V E +Q  P +        E F  K    P I++  P     P +
Sbjct: 337 --RDEHPAK--ASKTVVEGQQSSPILM-------ESFEKKNYSPPSIDAVGP----IPTK 396

Query: 301 KFGLKYEQEIISPPPSGSRTKSDVSLNLQDVLAAAQAAVETAERAAAAARSAASLTKVRI 360
           + G   +           R  SD   +LQDVL AAQAA ++AERAA+AARSAASL ++RI
Sbjct: 397 ESGASRDT---------PRKISDG--DLQDVLMAAQAAADSAERAASAARSAASLAQLRI 456

Query: 361 DTLTMKTNDQDPEICCENPFHG--------------------------ATTS-------- 420
           + LT KT+DQ PE   ENPFH                           A TS        
Sbjct: 457 NELTRKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSSGDLTELQRAETSSLFNSEQN 516

Query: 421 ---PPDHE----------------------QTFHLNQQDSIGHHSSSVPYY--------- 480
              P  H                       Q FH  ++    H +SS   Y         
Sbjct: 517 NQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFHSMEKPQFDHQNSSGSNYDDLTPQRFS 576

Query: 481 SQDSFQEHHKESPGSDVSD--HHRFES-QKSGFD------SSPGD---------SPSEHI 484
           S +  Q  H+ S GS+  D   HRF S +K  FD      SS GD         SP + +
Sbjct: 577 SLEKPQFDHQNSSGSNYDDLTPHRFPSMEKPQFDHQNSSVSSYGDLPELQRPETSPLDRL 613

BLAST of Carg28172 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-12
Identity = 57/149 (38.26%), Postives = 78/149 (52.35%), Query Frame = 0

Query: 3   LDLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLR-LCGTHFSATSE 62
           +DLKE I+S++F   PRC+++ EL  +R I   +    +    A DLR  CG +      
Sbjct: 114 VDLKEGIASLIF-AAPRCSEIPELGDLRDIFA-KKYGKDFVSAATDLRPSCGVN------ 173

Query: 63  LSLFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAEF 122
                        RM      LI+ LSVR P  E KLK++KEIA+E  VDWD   TE E 
Sbjct: 174 -------------RM------LIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL 233

Query: 123 NKSPEDLLNGSAQFVSASKLPLPQEKHNE 151
            K  E+ ++G  +FVSAS LP+ +   NE
Sbjct: 234 LKPQEESIDGPRKFVSASSLPVNRAAINE 235

BLAST of Carg28172 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.0e-10
Identity = 47/131 (35.88%), Postives = 73/131 (55.73%), Query Frame = 0

Query: 4   DLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATSELS 63
           +L+EAI+S++F   PRC+++ +LLQ++                    L GT +       
Sbjct: 101 ELREAIASIIF-AAPRCSEVPDLLQIK-------------------NLFGTKYGKE---- 160

Query: 64  LFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAEFNK 123
            F++V+   +    V    +IE LS  +PS   +LK+LKEIA+E+ ++WD + TEAEF K
Sbjct: 161 -FIMVASELRPDSGVNRT-IIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEAEFMK 205

Query: 124 SPEDLLNGSAQ 135
           S EDLL G+ Q
Sbjct: 221 SHEDLLGGAKQ 205

BLAST of Carg28172 vs. TAIR 10
Match: AT1G25420.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.0e-10
Identity = 47/131 (35.88%), Postives = 73/131 (55.73%), Query Frame = 0

Query: 4   DLKEAISSVLFLLLPRCADLTELLQVRKISCLRLISHNRTLMAADLRLCGTHFSATSELS 63
           +L+EAI+S++F   PRC+++ +LLQ++                    L GT +       
Sbjct: 33  ELREAIASIIF-AAPRCSEVPDLLQIK-------------------NLFGTKYGKE---- 92

Query: 64  LFVLVSLTAKRRMLVPLVPLIELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAEFNK 123
            F++V+   +    V    +IE LS  +PS   +LK+LKEIA+E+ ++WD + TEAEF K
Sbjct: 93  -FIMVASELRPDSGVNRT-IIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEAEFMK 137

Query: 124 SPEDLLNGSAQ 135
           S EDLL G+ Q
Sbjct: 153 SHEDLLGGAKQ 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025137.11.7e-268100.00hypothetical protein SDJN02_13960, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6592298.12.7e-22688.27IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022930138.11.1e-22487.65uncharacterized protein LOC111436655 isoform X1 [Cucurbita moschata] >XP_0229301... [more]
XP_023536600.12.1e-22387.45uncharacterized protein LOC111797723 [Cucurbita pepo subsp. pepo][more]
XP_022974062.19.0e-21484.16uncharacterized protein LOC111472688 [Cucurbita maxima] >XP_022974064.1 uncharac... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1EU695.5e-22587.65uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I9854.3e-21484.16uncharacterized protein LOC111472688 OS=Cucurbita maxima OX=3661 GN=LOC111472688... [more]
A0A0A0KBZ01.4e-15965.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1[more]
A0A5D3D4I01.4e-15665.38IST1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold607G00... [more]
A0A1S4E4M81.4e-15665.38uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=... [more]
Match NameE-valueIdentityDescription
AT1G34220.17.9e-5137.12Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.25.7e-4937.33Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.5e-1238.26Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.14.0e-1035.88Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.24.0e-1035.88Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 320..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..483
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 404..427
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 428..446
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 373..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 462..483
NoneNo IPR availablePANTHERPTHR12161:SF62REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 3..472
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 2..118
e-value: 2.5E-13
score: 51.9
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 3..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28172-RACarg28172-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport