Carg28171 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg28171
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr10: 5322780 .. 5323993 (-)
RNA-Seq ExpressionCarg28171
SyntenyCarg28171
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAGAGAGAGAGAGAGAAAGCTCCAAGAATTTGCAGAGAGAAACAGAAAATCCCTCCCCCCATTTCCCAAATCTCAGCGAGAGGAACAAGAAGAAGAAGAAGAAGAAGAAGAATCATCAACAATGGGGGACATGATTGTGAACAGCCAATACGAAATCCTGGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTCTGCCCCATTTCCAACAAGTTTTTCGCCTGCAAAACCATCGCCAAAAGCGATCTCATCGATGAAACCGACAAAGAATGCCTCGAGAAAGAGCCTAAAATCATGGCTCTTCTCCCTCCCCATCCCAATATTCTTCAGTTGATTGACGTTTTCGAGAACGATGATTATCTCTGTTTGATCTCTGAGCTCTGTGACTCCGTTTCCCTCTACGATCGGATTATACGTCGTCCTTTTTCCGAATCAGAAGCCGCCATCGTTATGAAGCAGCTTCTTCAAGCCCTCGCTCACTGCCATTACCATGGCGTTGTTCATCGCGATGTTAAGCCAGATAATGTGCTCTTCGATTCCAGAGACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGCGACAAAGACGGATTTACCTTCGGCGTCGTCGGAACGCCTTACTACATCGCGCCTGAAGTTTTGAGAGGATCCGAATACGGTCAGAAAGTCGATGTATGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCTGGATTCCCTCCGTTCTACGGCGACTCCGCGGCAGAGGTCTTTGAGGCCGTTCTCAGAGGAAATCTGAGATTTCCACCTAGGGTTTTCAGGTCGATTTCCCCTGCGGCTAAGGATTTAATGAAGAAGATGATATGCCGAGATGTCTCTAGAAGATTCTCCGCCGAACAAGCTCTCAGTAAGTACAACTTCCTCTAATTAAACACTGATTTCTGTTATTGAATGAAACCATTATACACTCTTTGATCTGATTGTTCTTGGATCTCTGAACTCCAAACTGTTGGTTTCCATATTCGTATACAAATCTTGAACCATTAGAAGATTCATTCATTACAGATTTGAGTTTAGAGCTGTTTGTTTGTTTGTTTGGTTCCTTATGTTGAATTGAAAATTGTTGTTAACAGGACACCCATGGATTATGAGTGGAGGAGAAGGAACATCATCAGTGGAGTAAATCGTTTAACATTTTGACTCACAAAAAAAAA

mRNA sequence

AGAGAGAGAGAGAGAGAGAGAAAGCTCCAAGAATTTGCAGAGAGAAACAGAAAATCCCTCCCCCCATTTCCCAAATCTCAGCGAGAGGAACAAGAAGAAGAAGAAGAAGAAGAAGAATCATCAACAATGGGGGACATGATTGTGAACAGCCAATACGAAATCCTGGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTCTGCCCCATTTCCAACAAGTTTTTCGCCTGCAAAACCATCGCCAAAAGCGATCTCATCGATGAAACCGACAAAGAATGCCTCGAGAAAGAGCCTAAAATCATGGCTCTTCTCCCTCCCCATCCCAATATTCTTCAGTTGATTGACGTTTTCGAGAACGATGATTATCTCTGTTTGATCTCTGAGCTCTGTGACTCCGTTTCCCTCTACGATCGGATTATACGTCGTCCTTTTTCCGAATCAGAAGCCGCCATCGTTATGAAGCAGCTTCTTCAAGCCCTCGCTCACTGCCATTACCATGGCGTTGTTCATCGCGATGTTAAGCCAGATAATGTGCTCTTCGATTCCAGAGACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGCGACAAAGACGGATTTACCTTCGGCGTCGTCGGAACGCCTTACTACATCGCGCCTGAAGTTTTGAGAGGATCCGAATACGGTCAGAAAGTCGATGTATGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCTGGATTCCCTCCGTTCTACGGCGACTCCGCGGCAGAGGTCTTTGAGGCCGTTCTCAGAGGAAATCTGAGATTTCCACCTAGGGTTTTCAGGTCGATTTCCCCTGCGGCTAAGGATTTAATGAAGAAGATGATATGCCGAGATGTCTCTAGAAGATTCTCCGCCGAACAAGCTCTCAGACACCCATGGATTATGAGTGGAGGAGAAGGAACATCATCAGTGGAGTAAATCGTTTAACATTTTGACTCACAAAAAAAAA

Coding sequence (CDS)

AGAGAGAGAGAGAGAGAGAGAAAGCTCCAAGAATTTGCAGAGAGAAACAGAAAATCCCTCCCCCCATTTCCCAAATCTCAGCGAGAGGAACAAGAAGAAGAAGAAGAAGAAGAAGAATCATCAACAATGGGGGACATGATTGTGAACAGCCAATACGAAATCCTGGAGGAGATCGGAAGAGGACGATTCGGAACCATCACTCGTTGCTTCTGCCCCATTTCCAACAAGTTTTTCGCCTGCAAAACCATCGCCAAAAGCGATCTCATCGATGAAACCGACAAAGAATGCCTCGAGAAAGAGCCTAAAATCATGGCTCTTCTCCCTCCCCATCCCAATATTCTTCAGTTGATTGACGTTTTCGAGAACGATGATTATCTCTGTTTGATCTCTGAGCTCTGTGACTCCGTTTCCCTCTACGATCGGATTATACGTCGTCCTTTTTCCGAATCAGAAGCCGCCATCGTTATGAAGCAGCTTCTTCAAGCCCTCGCTCACTGCCATTACCATGGCGTTGTTCATCGCGATGTTAAGCCAGATAATGTGCTCTTCGATTCCAGAGACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGCGACAAAGACGGATTTACCTTCGGCGTCGTCGGAACGCCTTACTACATCGCGCCTGAAGTTTTGAGAGGATCCGAATACGGTCAGAAAGTCGATGTATGGAGCGCCGGTGTGATTCTCTACACGATGTTGGCTGGATTCCCTCCGTTCTACGGCGACTCCGCGGCAGAGGTCTTTGAGGCCGTTCTCAGAGGAAATCTGAGATTTCCACCTAGGGTTTTCAGGTCGATTTCCCCTGCGGCTAAGGATTTAATGAAGAAGATGATATGCCGAGATGTCTCTAGAAGATTCTCCGCCGAACAAGCTCTCAGACACCCATGGATTATGAGTGGAGGAGAAGGAACATCATCAGTGGAGTAA

Protein sequence

RERERERKLQEFAERNRKSLPPFPKSQREEQEEEEEEEESSTMGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQALAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE
Homology
BLAST of Carg28171 vs. NCBI nr
Match: KAG7023891.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640.6 bits (1651), Expect = 7.3e-180
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   RERERERKLQEFAERNRKSLPPFPKSQREEQEEEEEEEESSTMGDMIVNSQYEILEEIGR 60
           RERERERKLQEFAERNRKSLPPFPKSQREEQEEEEEEEESSTMGDMIVNSQYEILEEIGR
Sbjct: 1   RERERERKLQEFAERNRKSLPPFPKSQREEQEEEEEEEESSTMGDMIVNSQYEILEEIGR 60

Query: 61  GRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHPNILQLIDVF 120
           GRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHPNILQLIDVF
Sbjct: 61  GRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHPNILQLIDVF 120

Query: 121 ENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQALAHCHYHGVVHRDVKPDN 180
           ENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQALAHCHYHGVVHRDVKPDN
Sbjct: 121 ENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQALAHCHYHGVVHRDVKPDN 180

Query: 181 VLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYGQKVDVWSAGVILY 240
           VLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYGQKVDVWSAGVILY
Sbjct: 181 VLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYGQKVDVWSAGVILY 240

Query: 241 TMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKMICRDVSRRFSAEQ 300
           TMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKMICRDVSRRFSAEQ
Sbjct: 241 TMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKMICRDVSRRFSAEQ 300

Query: 301 ALRHPWIMSGGEGTSSVE 319
           ALRHPWIMSGGEGTSSVE
Sbjct: 301 ALRHPWIMSGGEGTSSVE 318

BLAST of Carg28171 vs. NCBI nr
Match: XP_022961225.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Carg28171 vs. NCBI nr
Match: KAG6590287.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570.9 bits (1470), Expect = 7.1e-159
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTS VE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSPVE 276

BLAST of Carg28171 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 568.5 bits (1464), Expect = 3.5e-158
Identity = 274/276 (99.28%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSIS AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Carg28171 vs. NCBI nr
Match: XP_023534528.1 (phosphoenolpyruvate carboxylase kinase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.5 bits (1451), Expect = 1.1e-156
Identity = 271/276 (98.19%), Postives = 274/276 (99.28%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNIL LIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIV+KQLLQA
Sbjct: 61  IMALLPPHPNILPLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVIKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDN+LFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNLLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSIS AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Carg28171 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 5.4e-93
Identity = 163/276 (59.06%), Postives = 208/276 (75.36%), Query Frame = 0

Query: 40  SSTMGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEK 99
           S T+G+   N +Y+I EEIGRGRFGT++R + P +  FFACKTI K+ L D+ D+ CL+ 
Sbjct: 4   SQTLGNNNTN-KYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDN 63

Query: 100 EPKIMALLPPHPNILQLIDVFENDDYLCLISELC-DSVSLYDRIIRR-PFSESEAAIVMK 159
           EPK+MALL  HPNI+Q+ D+ + D  L +  EL   SVS+YDR++    F E + A   K
Sbjct: 64  EPKLMALLSYHPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAK 123

Query: 160 QLLQALAHCHYHGVVHRDVKPDNVLFDSR-DNLKLIDFGSAEWCDKDGFTFGVVGTPYYI 219
           Q+LQAL+HCH +GVVHRD+KP+N+L D R D +K+ DFGS  W  +   T GVVGTPYY+
Sbjct: 124 QILQALSHCHRYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYV 183

Query: 220 APEVLRGSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSI 279
           APEVL G  YG+KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFP ++FR +
Sbjct: 184 APEVLMGYSYGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGV 243

Query: 280 SPAAKDLMKKMICRDVSRRFSAEQALRHPWIMSGGE 313
           S  AKD ++K+IC+D SRRFSAEQALRHPWI   GE
Sbjct: 244 SSMAKDFLRKLICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Carg28171 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 5.0e-91
Identity = 160/268 (59.70%), Postives = 207/268 (77.24%), Query Frame = 0

Query: 48  VNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALL 107
           + + Y++ +EIGRGRFGTITRCF P + +F+ACKTI K  LID  D+EC+E EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 108 PPHPNILQLIDVFENDDYLCLISELCD-SVSLYDRIIRR--PFSESEAAIVMKQLLQALA 167
           PPHPNI+++ D++E +D L ++ EL D  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 168 HCHYHGVVHRDVKPDNVLFD-SRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRG 227
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYY+APEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 228 SEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDL 287
            +Y +KVD+WSAGV++YTMLAG PPF G++A ++FE++LRGNLRFPP+ F S+S  AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 288 MKKMICRDVSRRFSAEQALRHPWIMSGG 312
           ++KMICRDVSRRFSAE ALRH W+M+ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Carg28171 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.6e-58
Identity = 107/267 (40.07%), Postives = 174/267 (65.17%), Query Frame = 0

Query: 52  YEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHP 111
           Y + +E+GRG+FG    C    + K FACK+I+K  L+ + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 112 NILQLIDVFENDDYLCLISELCDSVSLYDRIIRR-PFSESEAAIVMKQLLQALAHCHYHG 171
           NI++    +E++  + L+ ELC    L+DRI+ +  +SE  AA V +Q++  +  CH+ G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 172 VVHRDVKPDNVLFDSRDN---LKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYG 231
           V+HRD+KP+N L  S+D    +K  DFG + + ++      +VG+ YY+APEVL+   YG
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 232 QKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKM 291
           +++D+WSAG+ILY +L+G PPF+ ++   +F+A+L G + F  + + SIS +AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 292 ICRDVSRRFSAEQALRHPWIMSGGEGT 315
           + +D  RR SA + L+HPW+  GGE +
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGGEAS 338

BLAST of Carg28171 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.8e-57
Identity = 116/268 (43.28%), Postives = 174/268 (64.93%), Query Frame = 0

Query: 48  VNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALL 107
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E +++E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 108 PPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRR-PFSESEAAIVMKQLLQALAHC 167
           P HPNI+ L + +E+D  + L+ ELC+   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 168 HYHGVVHRDVKPDNVLFDSRD---NLKLIDFGSAEWCDKDGFTFG-VVGTPYYIAPEVLR 227
           H HGV+HRD+KP+N LF ++    +LK IDFG + +  K G  F  +VG+PYY+APEVLR
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFF-KPGERFNEIVGSPYYMAPEVLR 229

Query: 228 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 287
            S YGQ++D+WSAGVILY +L G PPF+ ++   V +A+L+  + F    +  +S  AKD
Sbjct: 230 RS-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKD 289

Query: 288 LMKKMICRDVSRRFSAEQALRHPWIMSG 311
           L+KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 LIKKMLHPDPRRRLTAQQVLDHPWIQNG 315

BLAST of Carg28171 vs. ExPASy Swiss-Prot
Match: Q38868 (Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.8e-57
Identity = 107/267 (40.07%), Postives = 173/267 (64.79%), Query Frame = 0

Query: 52  YEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHP 111
           Y + +E+GRG+FG    C    + K +ACK+I+K  L+ + DK+ + +E +IM  L   P
Sbjct: 91  YTLGKELGRGQFGVTYLCTENSTGKKYACKSISKKKLVTKADKDDMRREIQIMQHLSGQP 150

Query: 112 NILQLIDVFENDDYLCLISELCDSVSLYDRIIRR-PFSESEAAIVMKQLLQALAHCHYHG 171
           NI++    +E++  + L+ ELC    L+DRII +  ++E  AA V +Q++  +  CH+ G
Sbjct: 151 NIVEFKGAYEDEKAVNLVMELCAGGELFDRIIAKGHYTERAAASVCRQIVNVVKICHFMG 210

Query: 172 VVHRDVKPDNVLFDSRDN---LKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYG 231
           V+HRD+KP+N L  S+D    +K  DFG + + ++      +VG+ YY+APEVLR   YG
Sbjct: 211 VLHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGKVYRDIVGSAYYVAPEVLR-RRYG 270

Query: 232 QKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKM 291
           ++VD+WSAG+ILY +L+G PPF+ ++   +F+A+L G++ F  + + SIS +AKDL+++M
Sbjct: 271 KEVDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGHIDFESQPWPSISSSAKDLVRRM 330

Query: 292 ICRDVSRRFSAEQALRHPWIMSGGEGT 315
           +  D  RR SA   L+HPW+  GGE +
Sbjct: 331 LTADPKRRISAADVLQHPWLREGGEAS 356

BLAST of Carg28171 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.0e-160
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Carg28171 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.7e-158
Identity = 274/276 (99.28%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSIS AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Carg28171 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.7e-146
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DLIDETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDV END YLCLIS+LC+ +SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRR SAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Carg28171 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.7e-146
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DLIDETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLPPHPNILQLIDV END YLCLIS+LC+ +SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRR SAEQALRHPWI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Carg28171 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.4e-144
Identity = 248/276 (89.86%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162
           IMALLP HPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 319
           LMKKMICRDVSRRFSAEQALRHPWI+SGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of Carg28171 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-94
Identity = 163/276 (59.06%), Postives = 208/276 (75.36%), Query Frame = 0

Query: 40  SSTMGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEK 99
           S T+G+   N +Y+I EEIGRGRFGT++R + P +  FFACKTI K+ L D+ D+ CL+ 
Sbjct: 4   SQTLGNNNTN-KYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDN 63

Query: 100 EPKIMALLPPHPNILQLIDVFENDDYLCLISELC-DSVSLYDRIIRR-PFSESEAAIVMK 159
           EPK+MALL  HPNI+Q+ D+ + D  L +  EL   SVS+YDR++    F E + A   K
Sbjct: 64  EPKLMALLSYHPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAK 123

Query: 160 QLLQALAHCHYHGVVHRDVKPDNVLFDSR-DNLKLIDFGSAEWCDKDGFTFGVVGTPYYI 219
           Q+LQAL+HCH +GVVHRD+KP+N+L D R D +K+ DFGS  W  +   T GVVGTPYY+
Sbjct: 124 QILQALSHCHRYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYV 183

Query: 220 APEVLRGSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSI 279
           APEVL G  YG+KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFP ++FR +
Sbjct: 184 APEVLMGYSYGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGV 243

Query: 280 SPAAKDLMKKMICRDVSRRFSAEQALRHPWIMSGGE 313
           S  AKD ++K+IC+D SRRFSAEQALRHPWI   GE
Sbjct: 244 SSMAKDFLRKLICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Carg28171 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 335.9 bits (860), Expect = 3.6e-92
Identity = 160/268 (59.70%), Postives = 207/268 (77.24%), Query Frame = 0

Query: 48  VNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALL 107
           + + Y++ +EIGRGRFGTITRCF P + +F+ACKTI K  LID  D+EC+E EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 108 PPHPNILQLIDVFENDDYLCLISELCD-SVSLYDRIIRR--PFSESEAAIVMKQLLQALA 167
           PPHPNI+++ D++E +D L ++ EL D  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 168 HCHYHGVVHRDVKPDNVLFD-SRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRG 227
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYY+APEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 228 SEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDL 287
            +Y +KVD+WSAGV++YTMLAG PPF G++A ++FE++LRGNLRFPP+ F S+S  AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 288 MKKMICRDVSRRFSAEQALRHPWIMSGG 312
           ++KMICRDVSRRFSAE ALRH W+M+ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Carg28171 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 325.1 bits (832), Expect = 6.3e-89
Identity = 156/266 (58.65%), Postives = 201/266 (75.56%), Query Frame = 0

Query: 40  SSTMGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEK 99
           S T+G+   N +Y+I EEIGRGRFGT++R + P +  FFACKTI K+ L D+ D+ CL+ 
Sbjct: 4   SQTLGNNNTN-KYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDN 63

Query: 100 EPKIMALLPPHPNILQLIDVFENDDYLCLISELC-DSVSLYDRIIRR-PFSESEAAIVMK 159
           EPK+MALL  HPNI+Q+ D+ + D  L +  EL   SVS+YDR++    F E + A   K
Sbjct: 64  EPKLMALLSYHPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAK 123

Query: 160 QLLQALAHCHYHGVVHRDVKPDNVLFDSR-DNLKLIDFGSAEWCDKDGFTFGVVGTPYYI 219
           Q+LQAL+HCH +GVVHRD+KP+N+L D R D +K+ DFGS  W  +   T GVVGTPYY+
Sbjct: 124 QILQALSHCHRYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYV 183

Query: 220 APEVLRGSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSI 279
           APEVL G  YG+KVD+WSAGV+LYTMLAG PPFYG++A E+FEAVLRGNLRFP ++FR +
Sbjct: 184 APEVLMGYSYGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGV 243

Query: 280 SPAAKDLMKKMICRDVSRRFSAEQAL 303
           S  AKD ++K+IC+D SRRFSAEQAL
Sbjct: 244 SSMAKDFLRKLICKDASRRFSAEQAL 268

BLAST of Carg28171 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 225.3 bits (573), Expect = 6.8e-59
Identity = 107/267 (40.07%), Postives = 174/267 (65.17%), Query Frame = 0

Query: 52  YEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALLPPHP 111
           Y + +E+GRG+FG    C    + K FACK+I+K  L+ + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 112 NILQLIDVFENDDYLCLISELCDSVSLYDRIIRR-PFSESEAAIVMKQLLQALAHCHYHG 171
           NI++    +E++  + L+ ELC    L+DRI+ +  +SE  AA V +Q++  +  CH+ G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 172 VVHRDVKPDNVLFDSRDN---LKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLRGSEYG 231
           V+HRD+KP+N L  S+D    +K  DFG + + ++      +VG+ YY+APEVL+   YG
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 232 QKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKDLMKKM 291
           +++D+WSAG+ILY +L+G PPF+ ++   +F+A+L G + F  + + SIS +AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 292 ICRDVSRRFSAEQALRHPWIMSGGEGT 315
           + +D  RR SA + L+HPW+  GGE +
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGGEAS 338

BLAST of Carg28171 vs. TAIR 10
Match: AT2G41860.1 (calcium-dependent protein kinase 14 )

HSP 1 Score: 223.8 bits (569), Expect = 2.0e-58
Identity = 116/268 (43.28%), Postives = 174/268 (64.93%), Query Frame = 0

Query: 48  VNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPKIMALL 107
           +  +Y++  E+GRG FG    C    + + FACK+I K  L    D E +++E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 108 PPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRR-PFSESEAAIVMKQLLQALAHC 167
           P HPNI+ L + +E+D  + L+ ELC+   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 168 HYHGVVHRDVKPDNVLFDSRD---NLKLIDFGSAEWCDKDGFTFG-VVGTPYYIAPEVLR 227
           H HGV+HRD+KP+N LF ++    +LK IDFG + +  K G  F  +VG+PYY+APEVLR
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFF-KPGERFNEIVGSPYYMAPEVLR 229

Query: 228 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 287
            S YGQ++D+WSAGVILY +L G PPF+ ++   V +A+L+  + F    +  +S  AKD
Sbjct: 230 RS-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKD 289

Query: 288 LMKKMICRDVSRRFSAEQALRHPWIMSG 311
           L+KKM+  D  RR +A+Q L HPWI +G
Sbjct: 290 LIKKMLHPDPRRRLTAQQVLDHPWIQNG 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023891.17.3e-180100.00Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022961225.11.9e-159100.00phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226... [more]
KAG6590287.17.1e-15999.64Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022988181.13.5e-15899.28phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
XP_023534528.11.1e-15698.19phosphoenolpyruvate carboxylase kinase 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SPK45.4e-9359.06Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK05.0e-9159.70Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q9C6P39.6e-5840.07Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
P937592.8e-5743.28Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
Q388684.8e-5740.07Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HBL39.0e-160100.00phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JGI41.7e-15899.28phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7TYV25.7e-14690.58Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK95.7e-14690.58phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A0A0M3G71.4e-14489.86Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
Match NameE-valueIdentityDescription
AT1G08650.13.8e-9459.06phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.13.6e-9259.70phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G08650.26.3e-8958.65phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G50700.16.8e-5940.07calcium-dependent protein kinase 33 [more]
AT2G41860.12.0e-5843.28calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 52..307
e-value: 2.1E-92
score: 323.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 52..307
e-value: 4.8E-72
score: 242.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 52..307
score: 48.004025
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 24..133
e-value: 1.7E-21
score: 78.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 136..316
e-value: 1.8E-65
score: 222.7
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 51..265
e-value: 3.0E-6
score: 24.3
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 49..249
e-value: 2.8E-8
score: 31.2
NoneNo IPR availablePIRSRPIRSR630220-1PIRSR630220-1coord: 11..256
e-value: 1.4E-8
score: 31.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 48..316
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 48..316
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 51..306
e-value: 2.28087E-124
score: 354.86
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 171..183
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 58..85
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 45..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28171-RACarg28171-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity