Carg28154 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28154
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Vacuolar iron transporter like
Location	Carg_Chr19: 2782198 .. 2782803 (+)
RNA-Seq Expression	Carg28154
Synteny	Carg28154

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTTCCATGCAGAAGCTTCCGAGTCTAGCAATGTTGATTACACTCAACGAGCTCAGTGGCTCCGAGCCGCAGTTCTCGGAGCCAACGACGGGCTCGTCTCCGTTGCATCGCTGATGATGGGCGTCGGCGCTGTGAAGCCCGACGCCAAAGCCATGCTTATTGCTGGATTTGCGGGGATGGTAGCGGGCGCTTGTAGTATGGCGATCGGAGAGTTCGTGTCGGTTTACACGCAATATGATATACAGAAAGCTCAGCTGAAGAGAGATAGGAAGCAAAAGAATGACATGCAGTCAAGCCAGTCGGCCGAGGAGGAAGAGAAGCTACCAAATCCGTTACAGGCCGCACTTGCATCAGCGATAGCTTTTACGGTCGGAGCGGTTATTCCGTTGCTGTCGGCAGTGTTTATACGAGACCACAAGGTAAGGCTCGGGGTGGTTGCAGCGGTGGCGAGCTTGACGTTGGTGGTTTTTGGAGGAGTTGGAGCCGTCCTAGGGAGGACTCCGGTGGGGAAATCGGCGGCGAGAGTGGTTGTTGGAGGGTGGATGGCAATGGGCATCACTTTTGGGCTTACAAAGCTGCTGGGGTCGAAGGGGCTGTGA

mRNA sequence

ATGGCTTCCTTCCATGCAGAAGCTTCCGAGTCTAGCAATGTTGATTACACTCAACGAGCTCAGTGGCTCCGAGCCGCAGTTCTCGGAGCCAACGACGGGCTCGTCTCCGTTGCATCGCTGATGATGGGCGTCGGCGCTGTGAAGCCCGACGCCAAAGCCATGCTTATTGCTGGATTTGCGGGGATGGTAGCGGGCGCTTGTAGTATGGCGATCGGAGAGTTCGTGTCGGTTTACACGCAATATGATATACAGAAAGCTCAGCTGAAGAGAGATAGGAAGCAAAAGAATGACATGCAGTCAAGCCAGTCGGCCGAGGAGGAAGAGAAGCTACCAAATCCGTTACAGGCCGCACTTGCATCAGCGATAGCTTTTACGGTCGGAGCGGTTATTCCGTTGCTGTCGGCAGTGTTTATACGAGACCACAAGGTAAGGCTCGGGGTGGTTGCAGCGGTGGCGAGCTTGACGTTGGTGGTTTTTGGAGGAGTTGGAGCCGTCCTAGGGAGGACTCCGGTGGGGAAATCGGCGGCGAGAGTGGTTGTTGGAGGGTGGATGGCAATGGGCATCACTTTTGGGCTTACAAAGCTGCTGGGGTCGAAGGGGCTGTGA

Coding sequence (CDS)

ATGGCTTCCTTCCATGCAGAAGCTTCCGAGTCTAGCAATGTTGATTACACTCAACGAGCTCAGTGGCTCCGAGCCGCAGTTCTCGGAGCCAACGACGGGCTCGTCTCCGTTGCATCGCTGATGATGGGCGTCGGCGCTGTGAAGCCCGACGCCAAAGCCATGCTTATTGCTGGATTTGCGGGGATGGTAGCGGGCGCTTGTAGTATGGCGATCGGAGAGTTCGTGTCGGTTTACACGCAATATGATATACAGAAAGCTCAGCTGAAGAGAGATAGGAAGCAAAAGAATGACATGCAGTCAAGCCAGTCGGCCGAGGAGGAAGAGAAGCTACCAAATCCGTTACAGGCCGCACTTGCATCAGCGATAGCTTTTACGGTCGGAGCGGTTATTCCGTTGCTGTCGGCAGTGTTTATACGAGACCACAAGGTAAGGCTCGGGGTGGTTGCAGCGGTGGCGAGCTTGACGTTGGTGGTTTTTGGAGGAGTTGGAGCCGTCCTAGGGAGGACTCCGGTGGGGAAATCGGCGGCGAGAGTGGTTGTTGGAGGGTGGATGGCAATGGGCATCACTTTTGGGCTTACAAAGCTGCTGGGGTCGAAGGGGCTGTGA

Protein sequence

MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAMGITFGLTKLLGSKGL

Homology

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BLAST of Carg28154 vs. NCBI nr
Match: KAG7011449.1 (Vacuolar iron transporter-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 8.3e-97
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120
           GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS
Sbjct: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120

Query: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180
           AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV
Sbjct: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180

Query: 181 GGWMAMGITFGLTKLLGSKGL 202
           GGWMAMGITFGLTKLLGSKGL
Sbjct: 181 GGWMAMGITFGLTKLLGSKGL 201

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BLAST of Carg28154 vs. NCBI nr
Match: XP_022963837.1 (vacuolar iron transporter homolog 2.1-like [Cucurbita moschata] >XP_023511630.1 vacuolar iron transporter homolog 2.1-like [Cucurbita pepo subsp. pepo] >KAG6571726.1 Vacuolar iron transporter-like 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.1 bits (928), Expect = 3.2e-96
Identity = 200/201 (99.50%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120
           GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSS SAEEEEKLPNPLQAALAS
Sbjct: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSHSAEEEEKLPNPLQAALAS 120

Query: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180
           AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV
Sbjct: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180

Query: 181 GGWMAMGITFGLTKLLGSKGL 202
           GGWMAMGITFGLTKLLGSKGL
Sbjct: 181 GGWMAMGITFGLTKLLGSKGL 201

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BLAST of Carg28154 vs. NCBI nr
Match: XP_022967511.1 (vacuolar iron transporter homolog 2.1-like [Cucurbita maxima])

HSP 1 Score: 357.5 bits (916), Expect = 7.8e-95
Identity = 198/201 (98.51%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120
           GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKN MQ S SAEEEEKLPNPLQAALAS
Sbjct: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNGMQPSHSAEEEEKLPNPLQAALAS 120

Query: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180
           AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV
Sbjct: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180

Query: 181 GGWMAMGITFGLTKLLGSKGL 202
           GGWMAMGITFGLTKLLGSKGL
Sbjct: 181 GGWMAMGITFGLTKLLGSKGL 201

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BLAST of Carg28154 vs. NCBI nr
Match: XP_038888849.1 (vacuolar iron transporter homolog 2.1-like [Benincasa hispida])

HSP 1 Score: 326.6 bits (836), Expect = 1.5e-85
Identity = 181/202 (89.60%), Postives = 193/202 (95.54%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASES +VDYTQRAQWLRAA+LGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESGSVDYTQRAQWLRAAILGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKND-MQSSQSAEEEEKLPNPLQAALA 120
           G+VAGACSMAIGEFVSVYTQYDI+KAQ+KRD K+KN+ MQ +  AEEEEKLPNPLQAALA
Sbjct: 61  GLVAGACSMAIGEFVSVYTQYDIEKAQMKRDGKEKNNSMQPNHQAEEEEKLPNPLQAALA 120

Query: 121 SAIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVV 180
           SAIAF+VGAVIPLL+AVFIRDHKVRLGVVAAVASLTLVVFG VGA+LGRTPV KSAARV+
Sbjct: 121 SAIAFSVGAVIPLLAAVFIRDHKVRLGVVAAVASLTLVVFGVVGAILGRTPVMKSAARVL 180

Query: 181 VGGWMAMGITFGLTKLLGSKGL 202
           VGGWMAM ITFGLTKLLGSKGL
Sbjct: 181 VGGWMAMAITFGLTKLLGSKGL 202

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BLAST of Carg28154 vs. NCBI nr
Match: XP_008449417.1 (PREDICTED: vacuolar iron transporter homolog 2.1-like [Cucumis melo] >KAA0057327.1 vacuolar iron transporter-like protein 2.1-like [Cucumis melo var. makuwa] >TYK13407.1 vacuolar iron transporter-like protein 2.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 312.4 bits (799), Expect = 2.9e-81
Identity = 173/195 (88.72%), Postives = 186/195 (95.38%), Query Frame = 0


Query: 8   ASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGAC 67
           ASES +VDYTQRAQWLRAA+LGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAG+VAGAC
Sbjct: 2   ASESGSVDYTQRAQWLRAAILGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGLVAGAC 61

Query: 68  SMAIGEFVSVYTQYDIQKAQLKRDRKQK-NDMQSSQSAEEEEKLPNPLQAALASAIAFTV 127
           SMAIGEFVSVYTQYDI+KAQLKR+ K+K N+M+  Q  EEEEKLPNPLQAALASAIAF+V
Sbjct: 62  SMAIGEFVSVYTQYDIEKAQLKRNGKEKNNNMEPKQPGEEEEKLPNPLQAALASAIAFSV 121

Query: 128 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 187
           GAVIPL++AVFIRDHKVRLGVVAAVASLTL+VFG VGA+LGRTPVGKSAARVVVGGWMAM
Sbjct: 122 GAVIPLVAAVFIRDHKVRLGVVAAVASLTLLVFGIVGAILGRTPVGKSAARVVVGGWMAM 181

Query: 188 GITFGLTKLLGSKGL 202
            ITFGLTKLLGSKGL
Sbjct: 182 AITFGLTKLLGSKGL 196

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BLAST of Carg28154 vs. ExPASy Swiss-Prot
Match: Q9LSF6 (Vacuolar iron transporter homolog 2.1 OS=Arabidopsis thaliana OX=3702 GN=VTL5 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.9e-61
Identity = 131/196 (66.84%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 9   SESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGACS 68
           +E   VDY QRAQWLRAA+LGANDGLV+VASLMMGVG++K D KAML+ GFAG+VAGACS
Sbjct: 22  AEKEEVDYMQRAQWLRAALLGANDGLVTVASLMMGVGSIKEDVKAMLLVGFAGLVAGACS 81

Query: 69  MAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEK---LPNPLQAALASAIAFT 128
           MAIGEFVSV TQ DI+ AQ+KR  + K  + +    EEEEK   LPNP QAA+ASA+AF+
Sbjct: 82  MAIGEFVSVCTQRDIETAQMKRAIEHKTSLSAIDEQEEEEKKERLPNPGQAAIASALAFS 141

Query: 129 VGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMA 188
           VGA +PLL AVFI +HKVR+ VVA VA++ LVVFG  GAVLG+T V KS+ RVV+GGWMA
Sbjct: 142 VGAAMPLLGAVFIENHKVRMVVVAVVATIALVVFGVTGAVLGKTSVVKSSVRVVIGGWMA 201

Query: 189 MGITFGLTKLLGSKGL 202
           M +TFGLTK +GS  +
Sbjct: 202 MALTFGLTKFIGSAAM 217

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BLAST of Carg28154 vs. ExPASy Swiss-Prot
Match: Q9M2C0 (Vacuolar iron transporter homolog 4 OS=Arabidopsis thaliana OX=3702 GN=At3g43660 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.0e-60
Identity = 121/195 (62.05%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  + +  DY++RAQWLRAAVLGANDGLVS ASLMMG+GAVK D + ML+ GFAG+VAGA
Sbjct: 13  EKDQETTFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQDVRIMLLTGFAGLVAGA 72

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEF+SVY+QYDI+ AQ+KR+         S    ++EKLP+P QAA+ASA+AFT+
Sbjct: 73  CSMAIGEFISVYSQYDIEVAQMKRE---------SGGETKKEKLPSPTQAAIASALAFTL 132

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PLL+A F++++KVR+GV+ A  +L LV+FG +GAVLG+ PV KS  RV++GGW+AM
Sbjct: 133 GAIVPLLAAAFVKEYKVRIGVIVAAVTLALVMFGWLGAVLGKAPVVKSLVRVLIGGWLAM 192

Query: 187 GITFGLTKLLGSKGL 202
            ITFG TKL+GS GL
Sbjct: 193 AITFGFTKLVGSHGL 198

[Close]

BLAST of Carg28154 vs. ExPASy Swiss-Prot
Match: Q9LPU9 (Vacuolar iron transporter homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VTL1 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.5e-59
Identity = 119/195 (61.03%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  +    DY++RAQWLRAAVLGANDGLVS ASLMMGVGAVK D K M+++GFAG+VAGA
Sbjct: 15  EMDQEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDVKVMILSGFAGLVAGA 74

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEFVSVY+QYDI+ AQ+KR+         +    E+EKLP+P+QAA ASA+AF++
Sbjct: 75  CSMAIGEFVSVYSQYDIEVAQMKRE---------NGGQVEKEKLPSPMQAAAASALAFSL 134

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PL++A F++D+ VR+G + A  +L LV+FG +GAVLG+ PV KS+ARV++GGW+AM
Sbjct: 135 GAIVPLMAAAFVKDYHVRIGAIVAAVTLALVMFGWLGAVLGKAPVFKSSARVLIGGWLAM 194

Query: 187 GITFGLTKLLGSKGL 202
            +TFGLTKL+G+  L
Sbjct: 195 AVTFGLTKLIGTHSL 200

[Close]

BLAST of Carg28154 vs. ExPASy Swiss-Prot
Match: Q9M2C3 (Vacuolar iron transporter homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At3g43630 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 8.7e-57
Identity = 118/192 (61.46%), Postives = 153/192 (79.69%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  +    DY++RAQWLRAAVLGANDGLVS ASLMMGVGAVK + K M++ GFAG+VAGA
Sbjct: 12  EKDQEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQNVKIMILTGFAGLVAGA 71

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEFVSVY+QYDI+ AQ+KR+         +    E+EKLP+P QAA ASA+AF++
Sbjct: 72  CSMAIGEFVSVYSQYDIEVAQMKRE---------TGGEIEKEKLPSPTQAAAASALAFSL 131

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PLL+A F++++KVR+G + A  +L LV+FG +GAVLG+ PV KS+ RV+VGGW+AM
Sbjct: 132 GAMVPLLAAAFVKEYKVRIGAIVAAVTLALVMFGWLGAVLGKAPVVKSSLRVLVGGWLAM 191

Query: 187 GITFGLTKLLGS 199
            IT+G TKL+GS
Sbjct: 192 AITYGFTKLIGS 194

[Close]

BLAST of Carg28154 vs. ExPASy Swiss-Prot
Match: Q9SRD3 (Vacuolar iron transporter homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VTL2 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-56
Identity = 117/192 (60.94%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 10  ESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGACSM 69
           ES+  DY++R+QWLRAAVLGANDGLVS ASLMMGVGAVK D KAM+++GFAGMVAGACSM
Sbjct: 15  ESTTFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKHDVKAMILSGFAGMVAGACSM 74

Query: 70  AIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTVGAV 129
           AIGEFVSVY+QYDI+ AQ++RD  +           E+EKLP+P+QAA ASA+AF+ GA+
Sbjct: 75  AIGEFVSVYSQYDIEVAQMERDSVE----------IEKEKLPSPMQAAAASALAFSAGAI 134

Query: 130 IPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAMGIT 189
           +PLL+A F++++K+R+  V    ++ L+VFG +GA LG+ P  +S+ARV+ GGW+AM +T
Sbjct: 135 VPLLAAAFVKEYKMRIISVVVAVTVALMVFGWLGAALGKAPAVRSSARVLFGGWLAMAVT 194

Query: 190 FGLTKLLGSKGL 202
           FGLTKL+G  GL
Sbjct: 195 FGLTKLIGLYGL 196

[Close]

BLAST of Carg28154 vs. ExPASy TrEMBL
Match: A0A6J1HGA3 (vacuolar iron transporter homolog 2.1-like OS=Cucurbita moschata OX=3662 GN=LOC111464025 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.5e-96
Identity = 200/201 (99.50%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120
           GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSS SAEEEEKLPNPLQAALAS
Sbjct: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSHSAEEEEKLPNPLQAALAS 120

Query: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180
           AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV
Sbjct: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180

Query: 181 GGWMAMGITFGLTKLLGSKGL 202
           GGWMAMGITFGLTKLLGSKGL
Sbjct: 181 GGWMAMGITFGLTKLLGSKGL 201

[Close]

BLAST of Carg28154 vs. ExPASy TrEMBL
Match: A0A6J1HSB3 (vacuolar iron transporter homolog 2.1-like OS=Cucurbita maxima OX=3661 GN=LOC111466995 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.8e-95
Identity = 198/201 (98.51%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60
           MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA
Sbjct: 1   MASFHAEASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFA 60

Query: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALAS 120
           GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKN MQ S SAEEEEKLPNPLQAALAS
Sbjct: 61  GMVAGACSMAIGEFVSVYTQYDIQKAQLKRDRKQKNGMQPSHSAEEEEKLPNPLQAALAS 120

Query: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180
           AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV
Sbjct: 121 AIAFTVGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVV 180

Query: 181 GGWMAMGITFGLTKLLGSKGL 202
           GGWMAMGITFGLTKLLGSKGL
Sbjct: 181 GGWMAMGITFGLTKLLGSKGL 201

[Close]

BLAST of Carg28154 vs. ExPASy TrEMBL
Match: A0A5D3CNF0 (Vacuolar iron transporter-like protein 2.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00220 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-81
Identity = 173/195 (88.72%), Postives = 186/195 (95.38%), Query Frame = 0


Query: 8   ASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGAC 67
           ASES +VDYTQRAQWLRAA+LGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAG+VAGAC
Sbjct: 2   ASESGSVDYTQRAQWLRAAILGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGLVAGAC 61

Query: 68  SMAIGEFVSVYTQYDIQKAQLKRDRKQK-NDMQSSQSAEEEEKLPNPLQAALASAIAFTV 127
           SMAIGEFVSVYTQYDI+KAQLKR+ K+K N+M+  Q  EEEEKLPNPLQAALASAIAF+V
Sbjct: 62  SMAIGEFVSVYTQYDIEKAQLKRNGKEKNNNMEPKQPGEEEEKLPNPLQAALASAIAFSV 121

Query: 128 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 187
           GAVIPL++AVFIRDHKVRLGVVAAVASLTL+VFG VGA+LGRTPVGKSAARVVVGGWMAM
Sbjct: 122 GAVIPLVAAVFIRDHKVRLGVVAAVASLTLLVFGIVGAILGRTPVGKSAARVVVGGWMAM 181

Query: 188 GITFGLTKLLGSKGL 202
            ITFGLTKLLGSKGL
Sbjct: 182 AITFGLTKLLGSKGL 196

[Close]

BLAST of Carg28154 vs. ExPASy TrEMBL
Match: A0A1S3BLD1 (vacuolar iron transporter homolog 2.1-like OS=Cucumis melo OX=3656 GN=LOC103491310 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-81
Identity = 173/195 (88.72%), Postives = 186/195 (95.38%), Query Frame = 0


Query: 8   ASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGAC 67
           ASES +VDYTQRAQWLRAA+LGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAG+VAGAC
Sbjct: 2   ASESGSVDYTQRAQWLRAAILGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGLVAGAC 61

Query: 68  SMAIGEFVSVYTQYDIQKAQLKRDRKQK-NDMQSSQSAEEEEKLPNPLQAALASAIAFTV 127
           SMAIGEFVSVYTQYDI+KAQLKR+ K+K N+M+  Q  EEEEKLPNPLQAALASAIAF+V
Sbjct: 62  SMAIGEFVSVYTQYDIEKAQLKRNGKEKNNNMEPKQPGEEEEKLPNPLQAALASAIAFSV 121

Query: 128 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 187
           GAVIPL++AVFIRDHKVRLGVVAAVASLTL+VFG VGA+LGRTPVGKSAARVVVGGWMAM
Sbjct: 122 GAVIPLVAAVFIRDHKVRLGVVAAVASLTLLVFGIVGAILGRTPVGKSAARVVVGGWMAM 181

Query: 188 GITFGLTKLLGSKGL 202
            ITFGLTKLLGSKGL
Sbjct: 182 AITFGLTKLLGSKGL 196

[Close]

BLAST of Carg28154 vs. ExPASy TrEMBL
Match: A0A0A0KGE6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411280 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.0e-81
Identity = 172/195 (88.21%), Postives = 186/195 (95.38%), Query Frame = 0


Query: 8   ASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGAC 67
           ASES +VDYTQRAQWLRAA+LGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAG+VAGAC
Sbjct: 2   ASESGSVDYTQRAQWLRAAILGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGLVAGAC 61

Query: 68  SMAIGEFVSVYTQYDIQKAQLKRDRKQK-NDMQSSQSAEEEEKLPNPLQAALASAIAFTV 127
           SMAIGEFVSVYTQYDI+KAQLKR+ K+K N+M+ +   EEEEKLPNPLQAALASAIAF+V
Sbjct: 62  SMAIGEFVSVYTQYDIEKAQLKRNGKEKNNNMEPNHPGEEEEKLPNPLQAALASAIAFSV 121

Query: 128 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 187
           GAVIPL++AVFIRDHKVRLGVVAAVASLTL+VFG VGA+LGRTPVGKSAARVVVGGWMAM
Sbjct: 122 GAVIPLVAAVFIRDHKVRLGVVAAVASLTLLVFGIVGAILGRTPVGKSAARVVVGGWMAM 181

Query: 188 GITFGLTKLLGSKGL 202
            ITFGLTKLLGSKGL
Sbjct: 182 AITFGLTKLLGSKGL 196

[Close]

BLAST of Carg28154 vs. TAIR 10
Match: AT3G25190.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 235.3 bits (599), Expect = 4.2e-62
Identity = 131/196 (66.84%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 9   SESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGACS 68
           +E   VDY QRAQWLRAA+LGANDGLV+VASLMMGVG++K D KAML+ GFAG+VAGACS
Sbjct: 22  AEKEEVDYMQRAQWLRAALLGANDGLVTVASLMMGVGSIKEDVKAMLLVGFAGLVAGACS 81

Query: 69  MAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEK---LPNPLQAALASAIAFT 128
           MAIGEFVSV TQ DI+ AQ+KR  + K  + +    EEEEK   LPNP QAA+ASA+AF+
Sbjct: 82  MAIGEFVSVCTQRDIETAQMKRAIEHKTSLSAIDEQEEEEKKERLPNPGQAAIASALAFS 141

Query: 129 VGAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMA 188
           VGA +PLL AVFI +HKVR+ VVA VA++ LVVFG  GAVLG+T V KS+ RVV+GGWMA
Sbjct: 142 VGAAMPLLGAVFIENHKVRMVVVAVVATIALVVFGVTGAVLGKTSVVKSSVRVVIGGWMA 201

Query: 189 MGITFGLTKLLGSKGL 202
           M +TFGLTK +GS  +
Sbjct: 202 MALTFGLTKFIGSAAM 217

[Close]

BLAST of Carg28154 vs. TAIR 10
Match: AT3G43660.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 232.3 bits (591), Expect = 3.5e-61
Identity = 121/195 (62.05%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  + +  DY++RAQWLRAAVLGANDGLVS ASLMMG+GAVK D + ML+ GFAG+VAGA
Sbjct: 13  EKDQETTFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQDVRIMLLTGFAGLVAGA 72

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEF+SVY+QYDI+ AQ+KR+         S    ++EKLP+P QAA+ASA+AFT+
Sbjct: 73  CSMAIGEFISVYSQYDIEVAQMKRE---------SGGETKKEKLPSPTQAAIASALAFTL 132

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PLL+A F++++KVR+GV+ A  +L LV+FG +GAVLG+ PV KS  RV++GGW+AM
Sbjct: 133 GAIVPLLAAAFVKEYKVRIGVIVAAVTLALVMFGWLGAVLGKAPVVKSLVRVLIGGWLAM 192

Query: 187 GITFGLTKLLGSKGL 202
            ITFG TKL+GS GL
Sbjct: 193 AITFGFTKLVGSHGL 198

[Close]

BLAST of Carg28154 vs. TAIR 10
Match: AT1G21140.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 228.8 bits (582), Expect = 3.9e-60
Identity = 119/195 (61.03%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  +    DY++RAQWLRAAVLGANDGLVS ASLMMGVGAVK D K M+++GFAG+VAGA
Sbjct: 15  EMDQEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDVKVMILSGFAGLVAGA 74

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEFVSVY+QYDI+ AQ+KR+         +    E+EKLP+P+QAA ASA+AF++
Sbjct: 75  CSMAIGEFVSVYSQYDIEVAQMKRE---------NGGQVEKEKLPSPMQAAAASALAFSL 134

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PL++A F++D+ VR+G + A  +L LV+FG +GAVLG+ PV KS+ARV++GGW+AM
Sbjct: 135 GAIVPLMAAAFVKDYHVRIGAIVAAVTLALVMFGWLGAVLGKAPVFKSSARVLIGGWLAM 194

Query: 187 GITFGLTKLLGSKGL 202
            +TFGLTKL+G+  L
Sbjct: 195 AVTFGLTKLIGTHSL 200

[Close]

BLAST of Carg28154 vs. TAIR 10
Match: AT3G43630.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 221.5 bits (563), Expect = 6.2e-58
Identity = 118/192 (61.46%), Postives = 153/192 (79.69%), Query Frame = 0


Query: 7   EASESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGA 66
           E  +    DY++RAQWLRAAVLGANDGLVS ASLMMGVGAVK + K M++ GFAG+VAGA
Sbjct: 12  EKDQEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQNVKIMILTGFAGLVAGA 71

Query: 67  CSMAIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTV 126
           CSMAIGEFVSVY+QYDI+ AQ+KR+         +    E+EKLP+P QAA ASA+AF++
Sbjct: 72  CSMAIGEFVSVYSQYDIEVAQMKRE---------TGGEIEKEKLPSPTQAAAASALAFSL 131

Query: 127 GAVIPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAM 186
           GA++PLL+A F++++KVR+G + A  +L LV+FG +GAVLG+ PV KS+ RV+VGGW+AM
Sbjct: 132 GAMVPLLAAAFVKEYKVRIGAIVAAVTLALVMFGWLGAVLGKAPVVKSSLRVLVGGWLAM 191

Query: 187 GITFGLTKLLGS 199
            IT+G TKL+GS
Sbjct: 192 AITYGFTKLIGS 194

[Close]

BLAST of Carg28154 vs. TAIR 10
Match: AT1G76800.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-57
Identity = 117/192 (60.94%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 10  ESSNVDYTQRAQWLRAAVLGANDGLVSVASLMMGVGAVKPDAKAMLIAGFAGMVAGACSM 69
           ES+  DY++R+QWLRAAVLGANDGLVS ASLMMGVGAVK D KAM+++GFAGMVAGACSM
Sbjct: 15  ESTTFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKHDVKAMILSGFAGMVAGACSM 74

Query: 70  AIGEFVSVYTQYDIQKAQLKRDRKQKNDMQSSQSAEEEEKLPNPLQAALASAIAFTVGAV 129
           AIGEFVSVY+QYDI+ AQ++RD  +           E+EKLP+P+QAA ASA+AF+ GA+
Sbjct: 75  AIGEFVSVYSQYDIEVAQMERDSVE----------IEKEKLPSPMQAAAASALAFSAGAI 134

Query: 130 IPLLSAVFIRDHKVRLGVVAAVASLTLVVFGGVGAVLGRTPVGKSAARVVVGGWMAMGIT 189
           +PLL+A F++++K+R+  V    ++ L+VFG +GA LG+ P  +S+ARV+ GGW+AM +T
Sbjct: 135 VPLLAAAFVKEYKMRIISVVVAVTVALMVFGWLGAALGKAPAVRSSARVLFGGWLAMAVT 194

Query: 190 FGLTKLLGSKGL 202
           FGLTKL+G  GL
Sbjct: 195 FGLTKLIGLYGL 196

The following BLAST results are available for this feature:

BLAST of Carg28154 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011449.1	8.3e-97	100.00	Vacuolar iron transporter-like 4, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022963837.1	3.2e-96	99.50	vacuolar iron transporter homolog 2.1-like [Cucurbita moschata] >XP_023511630.1 ...	[more]
XP_022967511.1	7.8e-95	98.51	vacuolar iron transporter homolog 2.1-like [Cucurbita maxima]	[more]
XP_038888849.1	1.5e-85	89.60	vacuolar iron transporter homolog 2.1-like [Benincasa hispida]	[more]
XP_008449417.1	2.9e-81	88.72	PREDICTED: vacuolar iron transporter homolog 2.1-like [Cucumis melo] >KAA0057327...	[more]

BLAST of Carg28154 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSF6	5.9e-61	66.84	Vacuolar iron transporter homolog 2.1 OS=Arabidopsis thaliana OX=3702 GN=VTL5 PE...	[more]
Q9M2C0	5.0e-60	62.05	Vacuolar iron transporter homolog 4 OS=Arabidopsis thaliana OX=3702 GN=At3g43660...	[more]
Q9LPU9	5.5e-59	61.03	Vacuolar iron transporter homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VTL1 PE=2...	[more]
Q9M2C3	8.7e-57	61.46	Vacuolar iron transporter homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At3g43630...	[more]
Q9SRD3	2.5e-56	60.94	Vacuolar iron transporter homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VTL2 PE=2...	[more]

BLAST of Carg28154 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HGA3	1.5e-96	99.50	vacuolar iron transporter homolog 2.1-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1HSB3	3.8e-95	98.51	vacuolar iron transporter homolog 2.1-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5D3CNF0	1.4e-81	88.72	Vacuolar iron transporter-like protein 2.1-like OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BLD1	1.4e-81	88.72	vacuolar iron transporter homolog 2.1-like OS=Cucumis melo OX=3656 GN=LOC1034913...	[more]
A0A0A0KGE6	4.0e-81	88.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411280 PE=3 SV=1	[more]

BLAST of Carg28154 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25190.1	4.2e-62	66.84	Vacuolar iron transporter (VIT) family protein	[more]
AT3G43660.1	3.5e-61	62.05	Vacuolar iron transporter (VIT) family protein	[more]
AT1G21140.1	3.9e-60	61.03	Vacuolar iron transporter (VIT) family protein	[more]
AT3G43630.1	6.2e-58	61.46	Vacuolar iron transporter (VIT) family protein	[more]
AT1G76800.1	1.8e-57	60.94	Vacuolar iron transporter (VIT) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008217	Ccc1 family	PFAM	PF01988	VIT1	coord: 23..103 e-value: 3.5E-26 score: 92.4 coord: 103..193 e-value: 4.9E-19 score: 69.1
IPR008217	Ccc1 family	PANTHER	PTHR31851	FE(2+)/MN(2+) TRANSPORTER PCL1	coord: 8..198
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 89..109
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 89..104
None	No IPR available	PANTHER	PTHR31851:SF60	VACUOLAR IRON TRANSPORTER HOMOLOG 2.1	coord: 8..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28154-RA	Carg28154-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030026	cellular manganese ion homeostasis
biological_process	GO:0006880	intracellular sequestering of iron ion
biological_process	GO:0034755	iron ion transmembrane transport
biological_process	GO:0071421	manganese ion transmembrane transport
biological_process	GO:0010039	response to iron ion
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005381	iron ion transmembrane transporter activity
molecular_function	GO:0005384	manganese ion transmembrane transporter activity