Carg28138 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg28138 vs. NCBI nr
Match: KAG7023997.1 (Calmodulin-binding protein 60 G, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 735.3 bits (1897), Expect = 2.5e-208
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL
Sbjct: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120
           SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI
Sbjct: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120

Query: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180
           KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG
Sbjct: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180

Query: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240
           KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV
Sbjct: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240

Query: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI 300
           SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI
Sbjct: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI 300

Query: 301 DFAAIVTREVSTSCDAAAATTMCKPQLCAAVICLLGGALTFSDAAAAAATTMCSHNYVQP 360
           DFAAIVTREVSTSCDAAAATTMCKPQLCAAVICLLGGALTFSDAAAAAATTMCSHNYVQP
Sbjct: 301 DFAAIVTREVSTSCDAAAATTMCKPQLCAAVICLLGGALTFSDAAAAAATTMCSHNYVQP 360

Query: 361 VIVYWSSFNFM 372
           VIVYWSSFNFM
Sbjct: 361 VIVYWSSFNFM 371

BLAST of Carg28138 vs. NCBI nr
Match: XP_022961387.1 (calmodulin-binding protein 60 G-like [Cucurbita moschata])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 335/445 (75.28%), Postives = 337/445 (75.73%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MDA  KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL
Sbjct: 1   MDA--KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120
           SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI
Sbjct: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120

Query: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180
           KAENGEALVV ICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG
Sbjct: 121 KAENGEALVVTICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180

Query: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240
           KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV
Sbjct: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240

Query: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI 300
           SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLS HGIKNVDDFLLEYQKI
Sbjct: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSSHGIKNVDDFLLEYQKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GPARLRKLLGNKVSDKTWIMAVENAFECQSYTIYEPFSETCFPANNVMGDETFNEVHDIL 360

BLAST of Carg28138 vs. NCBI nr
Match: KAG6590470.1 (Calmodulin-binding protein 60 G, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.8 bits (1257), Expect = 4.1e-134
Identity = 250/252 (99.21%), Postives = 250/252 (99.21%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MDA  KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL
Sbjct: 1   MDA--KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120
           SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI
Sbjct: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120

Query: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180
           KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG
Sbjct: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180

Query: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240
           KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV
Sbjct: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240

Query: 241 SQSFRVMDHRGE 253
           SQSFRVMDHRGE
Sbjct: 241 SQSFRVMDHRGE 250

BLAST of Carg28138 vs. NCBI nr
Match: XP_022961392.1 (calmodulin-binding protein 60 G-like [Cucurbita moschata])

HSP 1 Score: 471.9 bits (1213), Expect = 5.2e-129
Identity = 238/297 (80.13%), Postives = 260/297 (87.54%), Query Frame = 0

Query: 9   GKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNWVQVEV 68
           GKGK+KMPPNDDDHNPNPKRTRPSSSSGIG  SVAEDE +VLE LSRIQPILSNWV+V V
Sbjct: 3   GKGKSKMPPNDDDHNPNPKRTRPSSSSGIGDRSVAEDE-HVLEFLSRIQPILSNWVEVGV 62

Query: 69  MNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAENGEAL 128
           +NALQTHFHPLLE LRER E GEEAI EGG+EKFKLVFCNQP STIFTNN+IKAENGEAL
Sbjct: 63  INALQTHFHPLLELLRERIESGEEAIVEGGVEKFKLVFCNQPASTIFTNNKIKAENGEAL 122

Query: 129 VVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGKRPLLVGN 188
           VVAICDATTN IIRTG +ASA V L +VNGDYD TN S+SEL+RN+LS R+GKRPL+ G+
Sbjct: 123 VVAICDATTNDIIRTGLMASAAVELGLVNGDYDGTNRSVSELNRNVLSAREGKRPLMHGD 182

Query: 189 GIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQSFRVMD 248
           G K  LENG A I CV ITDNSSW KSKKFR++AK IDEK+LAMFPT+G AVSQSFRVMD
Sbjct: 183 GTKFLLENGYAPINCVCITDNSSWTKSKKFRIIAKIIDEKILAMFPTIGVAVSQSFRVMD 242

Query: 249 HRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKIDFAAI 306
           HRGEVNKKHHPPSREDEVWRLEGI KDG YH+NLS HGIKNV DFLL+Y +I  A +
Sbjct: 243 HRGEVNKKHHPPSREDEVWRLEGIGKDGAYHKNLSSHGIKNVGDFLLKYHEIGSALL 298

BLAST of Carg28138 vs. NCBI nr
Match: KAG7023998.1 (Calmodulin-binding protein 60 G, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 471.9 bits (1213), Expect = 5.2e-129
Identity = 245/263 (93.16%), Postives = 248/263 (94.30%), Query Frame = 0

Query: 4   KGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNW 63
           KGKGKGK + +            + TRPSSSSGIGATSVAEDERYVLECLSRIQPILSNW
Sbjct: 40  KGKGKGKARPRCLLMMMITTLTLRETRPSSSSGIGATSVAEDERYVLECLSRIQPILSNW 99

Query: 64  VQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAE 123
           VQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAE
Sbjct: 100 VQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAE 159

Query: 124 NGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGKRP 183
           NGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGKRP
Sbjct: 160 NGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGKRP 219

Query: 184 LLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQS 243
           LLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQS
Sbjct: 220 LLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQS 279

Query: 244 FRVMDHRGEVNKKHHPPSREDEV 267
           FRVMDHRGEVNKKHHPPSREDEV
Sbjct: 280 FRVMDHRGEVNKKHHPPSREDEV 302

BLAST of Carg28138 vs. ExPASy Swiss-Prot
Match: F4K2R6 (Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.8e-38
Identity = 95/235 (40.43%), Postives = 133/235 (56.60%), Query Frame = 0

Query: 60  LSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNE 119
           + N ++  V   +Q    P L S     E            + KL F N PPS+IFT ++
Sbjct: 45  MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSET-PSSRSRLKLCFINSPPSSIFTGSK 104

Query: 120 IKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRD 179
           I+AE+G  LV+ + DATTN ++ TG  +S+ V LV +N D+ E + ++   +RNIL+ R+
Sbjct: 105 IEAEDGSPLVIELVDATTNTLVSTGPFSSSRVELVPLNADFTEESWTVEGFNRNILTQRE 164

Query: 180 GKRPLLVGNGIKLNLENGVAFIK-CVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGF 239
           GKRPLL G+ + + L+NGV  I   ++ +DNSSW +S+KFRL AK   +  +        
Sbjct: 165 GKRPLLTGD-LTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAVE------- 224

Query: 240 AVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDF 294
           A S++F   D RGE  KKHHPP   DEVWRLE I KDG     L+   I  V DF
Sbjct: 225 ARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDF 270

BLAST of Carg28138 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.5e-35
Identity = 89/243 (36.63%), Postives = 138/243 (56.79%), Query Frame = 0

Query: 56  IQPILSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIF 115
           ++P++   V+ EV  AL  H    ++ + E+     E+         +L F N     +F
Sbjct: 32  LEPLIRKVVKEEVELALGKHLAG-IKWICEKETHPLES------RNLQLKFLNNLSLPVF 91

Query: 116 TNNEIKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSE-LSRNI 175
           T+  I+ + G+A+ V + D +T  I  +G  +SA + + VV GD++  +    E +  NI
Sbjct: 92  TSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVEGDFNSVSDWTDEDIRNNI 151

Query: 176 LSPRDGKRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFP 235
           +  R+GK+PLL GN   + L +G+  +  +S TDNSSW +S+KFRL  + +D+     + 
Sbjct: 152 VREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---FDYV 211

Query: 236 TVGFAVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
            +  A+++SF V DHRGE+ KKHHPPS  DEVWRLE I KDG +HR L+   I  V DFL
Sbjct: 212 KIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVKDFL 263

Query: 296 LEY 298
             +
Sbjct: 272 THF 263

BLAST of Carg28138 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.3e-33
Identity = 101/287 (35.19%), Postives = 154/287 (53.66%), Query Frame = 0

Query: 12  KAKMPPNDD-DHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNWVQVEVMN 71
           K K+  +D+      P+R RP+ +S I   ++  D   +    S ++PIL   V  EV  
Sbjct: 15  KRKLEEDDNQQQQQQPERKRPALASVI-VEALKMDS--LQRLCSSLEPILRRVVSEEVER 74

Query: 72  ALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAENGEALVV 131
           AL       L   R       + I   G    +L F ++    +FT  +I+ E G A+ V
Sbjct: 75  ALAK-----LGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTGGKIEGEQGAAIHV 134

Query: 132 AICDATTNAIIRTGRLASALVGLVVVNGDY---DETNSSLSELSRNILSPRDGKRPLLVG 191
            + D TT  ++  G  ASA + +VV++GD+   D+   S  E   +++  R GKRPLL G
Sbjct: 135 VLLDMTTGHVLTVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGHLVKERQGKRPLLTG 194

Query: 192 NGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQSFRVM 251
           + +++ L+ GV  +  +  TDNSSW++ +KFRL  +        M   V  A +++F V 
Sbjct: 195 D-VQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM--RVREAKTEAFTVK 254

Query: 252 DHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
           DHRGE+ KKH+PP+ +DEVWRLE I KDG +H+ L+  GI NV +FL
Sbjct: 255 DHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 290

BLAST of Carg28138 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.6e-32
Identity = 99/298 (33.22%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MD+      + K  +  NDDD    P+R RP+ +S I      +    + +  S ++PIL
Sbjct: 2   MDSGNNNMNRAKRNLDGNDDD---QPERKRPAFASVIVEALKVDS---LQKLCSSLEPIL 61

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEG-GIEKFKLVFCNQPPSTIFTNNE 120
              V  E+  AL      L  +      G      EG    K +L F ++    +FT  +
Sbjct: 62  RRVVSEELERALA----KLGPARLTGSSGSSPKRIEGPDGRKLQLHFKSRLSLPLFTGGK 121

Query: 121 IKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDY---DETNSSLSELSRNILS 180
           ++ E G  + V + DA T   +  G  ASA + +VV+ GD+   D+ + +  E   +++ 
Sbjct: 122 VEGEQGAVIHVVLIDANTGRAVVYGPEASAKLHIVVLEGDFNTEDDEDWTQEEFESHVVK 181

Query: 181 PRDGKRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTV 240
            R GKRPLL G  + + L+ GV  +  +  TDNSSW++S+KFRL  + +      M   +
Sbjct: 182 ERSGKRPLLTGE-VYVTLKEGVGTLGELVFTDNSSWIRSRKFRLGLRVVSGCCDGM--RI 241

Query: 241 GFAVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
             A +++F V DHRGE+ KKH+PP+  D+VWRL+ I KDG +H+ L+  GI  V+DFL
Sbjct: 242 REAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGKDGAFHKKLTAEGINTVEDFL 286

BLAST of Carg28138 vs. ExPASy Swiss-Prot
Match: Q0WVV6 (Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.0e-30
Identity = 96/289 (33.22%), Postives = 149/289 (51.56%), Query Frame = 0

Query: 12  KAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNWVQVEVMNA 71
           K     NDDD    P+R RP+ +S I      +    + +  S ++PIL   V  EV  A
Sbjct: 2   KRNFERNDDD---KPERKRPALASVIVEALKVDS---LQKLCSSLEPILRRVVSEEVERA 61

Query: 72  LQTHFHPLLES---LRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAENGEAL 131
           L       L +      +R GG +          +L F ++    +FT   ++ E G  +
Sbjct: 62  LAKLVPTRLTTSSVFSPKRIGGPDG------RNLQLHFKSRLSLPLFTGGRVEGEQGATI 121

Query: 132 VVAICDATTNAIIRTGRLASALVGLVVVNGDY---DETNSSLSELSRNILSPRDGKRPLL 191
            V + DA T   +  G  AS  + +VV+ GD+   D+ + +  E   +++  R+GKRPLL
Sbjct: 122 HVVLIDANTGRPVTVGPEASLKLEVVVLGGDFNNEDDEDWTQEEFESHVVKEREGKRPLL 181

Query: 192 VGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQSFR 251
            G+ + + L+ GV  +  +  TDNSSW++S+KFRL  +        +   +  A +++F 
Sbjct: 182 TGD-LFVVLKEGVGTLGEIVFTDNSSWIRSRKFRLGLRVPSGYCDGI--RIREAKTEAFS 241

Query: 252 VMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
           V DHRGE+ KKH+PP+  DEVWRLE I KDG +H+ L+  GI  V+ FL
Sbjct: 242 VKDHRGELYKKHYPPALNDEVWRLEKIGKDGAFHKRLTAAGIVTVEGFL 275

BLAST of Carg28138 vs. ExPASy TrEMBL
Match: A0A6J1HA25 (calmodulin-binding protein 60 G-like OS=Cucurbita moschata OX=3662 GN=LOC111461976 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.4e-172
Identity = 335/445 (75.28%), Postives = 337/445 (75.73%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MDA  KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL
Sbjct: 1   MDA--KGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120
           SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI
Sbjct: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120

Query: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180
           KAENGEALVV ICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG
Sbjct: 121 KAENGEALVVTICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180

Query: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240
           KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV
Sbjct: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240

Query: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI 300
           SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLS HGIKNVDDFLLEYQKI
Sbjct: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSSHGIKNVDDFLLEYQKI 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GPARLRKLLGNKVSDKTWIMAVENAFECQSYTIYEPFSETCFPANNVMGDETFNEVHDIL 360

BLAST of Carg28138 vs. ExPASy TrEMBL
Match: A0A6J1HA30 (calmodulin-binding protein 60 G-like OS=Cucurbita moschata OX=3662 GN=LOC111461978 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.5e-129
Identity = 238/297 (80.13%), Postives = 260/297 (87.54%), Query Frame = 0

Query: 9   GKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNWVQVEV 68
           GKGK+KMPPNDDDHNPNPKRTRPSSSSGIG  SVAEDE +VLE LSRIQPILSNWV+V V
Sbjct: 3   GKGKSKMPPNDDDHNPNPKRTRPSSSSGIGDRSVAEDE-HVLEFLSRIQPILSNWVEVGV 62

Query: 69  MNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAENGEAL 128
           +NALQTHFHPLLE LRER E GEEAI EGG+EKFKLVFCNQP STIFTNN+IKAENGEAL
Sbjct: 63  INALQTHFHPLLELLRERIESGEEAIVEGGVEKFKLVFCNQPASTIFTNNKIKAENGEAL 122

Query: 129 VVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGKRPLLVGN 188
           VVAICDATTN IIRTG +ASA V L +VNGDYD TN S+SEL+RN+LS R+GKRPL+ G+
Sbjct: 123 VVAICDATTNDIIRTGLMASAAVELGLVNGDYDGTNRSVSELNRNVLSAREGKRPLMHGD 182

Query: 189 GIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQSFRVMD 248
           G K  LENG A I CV ITDNSSW KSKKFR++AK IDEK+LAMFPT+G AVSQSFRVMD
Sbjct: 183 GTKFLLENGYAPINCVCITDNSSWTKSKKFRIIAKIIDEKILAMFPTIGVAVSQSFRVMD 242

Query: 249 HRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKIDFAAI 306
           HRGEVNKKHHPPSREDEVWRLEGI KDG YH+NLS HGIKNV DFLL+Y +I  A +
Sbjct: 243 HRGEVNKKHHPPSREDEVWRLEGIGKDGAYHKNLSSHGIKNVGDFLLKYHEIGSALL 298

BLAST of Carg28138 vs. ExPASy TrEMBL
Match: A0A6J1HVP7 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467877 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.5e-126
Identity = 262/403 (65.01%), Postives = 288/403 (71.46%), Query Frame = 0

Query: 8   KGKGKAKMPPNDDDHNPNPKRTR------PSSSSGIGATSVAEDERYVLECLSRIQPILS 67
           KGKGKAKMPPNDDD NPNPKRTR      PSSSSGIGA SVAEDE +VLE LSRIQPILS
Sbjct: 4   KGKGKAKMPPNDDDDNPNPKRTRPSSSSGPSSSSGIGARSVAEDE-HVLEFLSRIQPILS 63

Query: 68  NWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIK 127
           NWV+V VMN LQTHFHPLLE LRER E GEEAI EGG+EKFKLVFCNQP STIFTNN+IK
Sbjct: 64  NWVEVGVMNVLQTHFHPLLELLRERIESGEEAIVEGGLEKFKLVFCNQPASTIFTNNKIK 123

Query: 128 AENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGK 187
           AENGEALVVAICDATTN II TG +ASA V L +VNGDYD TN SLSEL+RN+LS R+GK
Sbjct: 124 AENGEALVVAICDATTNDIICTGLMASAAVELGLVNGDYDGTNPSLSELNRNVLSAREGK 183

Query: 188 RPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVS 247
           RPL+ G+G K  LENG A I CV ITDNSSW KSKKFR++AK  DEK+LAMFPT+G AVS
Sbjct: 184 RPLMHGDGTKFLLENGYAPINCVCITDNSSWTKSKKFRMIAKIADEKILAMFPTIGVAVS 243

Query: 248 QSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKID 307
           QSFRVMDHRGEVNKKHHPPSREDEVWRLEGI KDG YH+NLS HGIK VDDFLL+Y +I 
Sbjct: 244 QSFRVMDHRGEVNKKHHPPSREDEVWRLEGIGKDGAYHKNLSSHGIKIVDDFLLKYHEIG 303

Query: 308 FAAIV------------TREVSTSCDAAAATT---------------------------- 358
            A +             T  V  + +  + TT                            
Sbjct: 304 SAPLKKLLGNKVPDKTWTMMVENAFECQSYTTSEPFSKAYLPGNNVMEDGALNEVFDIFE 363

BLAST of Carg28138 vs. ExPASy TrEMBL
Match: A0A6J1HUC0 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467877 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.6e-126
Identity = 239/304 (78.62%), Postives = 258/304 (84.87%), Query Frame = 0

Query: 8   KGKGKAKMPPNDDDHNPNPKRTR------PSSSSGIGATSVAEDERYVLECLSRIQPILS 67
           KGKGKAKMPPNDDD NPNPKRTR      PSSSSGIGA SVAEDE +VLE LSRIQPILS
Sbjct: 4   KGKGKAKMPPNDDDDNPNPKRTRPSSSSGPSSSSGIGARSVAEDE-HVLEFLSRIQPILS 63

Query: 68  NWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIK 127
           NWV+V VMN LQTHFHPLLE LRER E GEEAI EGG+EKFKLVFCNQP STIFTNN+IK
Sbjct: 64  NWVEVGVMNVLQTHFHPLLELLRERIESGEEAIVEGGLEKFKLVFCNQPASTIFTNNKIK 123

Query: 128 AENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDGK 187
           AENGEALVVAICDATTN II TG +ASA V L +VNGDYD TN SLSEL+RN+LS R+GK
Sbjct: 124 AENGEALVVAICDATTNDIICTGLMASAAVELGLVNGDYDGTNPSLSELNRNVLSAREGK 183

Query: 188 RPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVS 247
           RPL+ G+G K  LENG A I CV ITDNSSW KSKKFR++AK  DEK+LAMFPT+G AVS
Sbjct: 184 RPLMHGDGTKFLLENGYAPINCVCITDNSSWTKSKKFRMIAKIADEKILAMFPTIGVAVS 243

Query: 248 QSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKID 306
           QSFRVMDHRGEVNKKHHPPSREDEVWRLEGI KDG YH+NLS HGIK VDDFLL+Y +I 
Sbjct: 244 QSFRVMDHRGEVNKKHHPPSREDEVWRLEGIGKDGAYHKNLSSHGIKIVDDFLLKYHEIG 303

BLAST of Carg28138 vs. ExPASy TrEMBL
Match: A0A6J1HC19 (calmodulin-binding protein 60 A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461964 PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 2.1e-120
Identity = 231/305 (75.74%), Postives = 257/305 (84.26%), Query Frame = 0

Query: 1   MDAKGKGKGKGKAKMPPNDDDHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPIL 60
           MDA  KGKGK KAKMPPNDDD NPNPKRTRPSSS    A +VAED+R +++ LSRIQP+L
Sbjct: 1   MDA--KGKGKAKAKMPPNDDD-NPNPKRTRPSSSR-TKAGTVAEDDRSLVDLLSRIQPLL 60

Query: 61  SNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEI 120
           SN V  EV NALQTH HPLL+SLRER+E GEEAI EGGIEKFKL FCNQP +TIFTNNEI
Sbjct: 61  SNLVHKEVKNALQTHVHPLLKSLRERKEMGEEAIVEGGIEKFKLAFCNQPANTIFTNNEI 120

Query: 121 KAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRDG 180
           KAENGEA+ VAI D TTNAI+RTG L+SALVGL++++GDYDETN SLSEL RNILSPRDG
Sbjct: 121 KAENGEAVRVAIYDTTTNAIVRTGPLSSALVGLILLDGDYDETNRSLSELKRNILSPRDG 180

Query: 181 KRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAV 240
           KRPLLVG+GIKL LENG A IKCV ITDNSSWMKSKKFRL    IDE +LAM+PT+G AV
Sbjct: 181 KRPLLVGDGIKLILENGFASIKCVCITDNSSWMKSKKFRLGVMIIDENILAMYPTIGAAV 240

Query: 241 SQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFLLEYQKI 300
           SQ FR MDHRGEVNKKHHPPS EDEVWRLEGI KDG YH+NLS HGIK V  F+L++Q+I
Sbjct: 241 SQPFRAMDHRGEVNKKHHPPSIEDEVWRLEGIGKDGAYHKNLSSHGIKTVGAFVLKHQEI 300

Query: 301 DFAAI 306
              A+
Sbjct: 301 GPTAL 301

BLAST of Carg28138 vs. TAIR 10
Match: AT5G26920.1 (Cam-binding protein 60-like G )

HSP 1 Score: 159.8 bits (403), Expect = 4.1e-39
Identity = 95/235 (40.43%), Postives = 133/235 (56.60%), Query Frame = 0

Query: 60  LSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNE 119
           + N ++  V   +Q    P L S     E            + KL F N PPS+IFT ++
Sbjct: 45  MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSET-PSSRSRLKLCFINSPPSSIFTGSK 104

Query: 120 IKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRD 179
           I+AE+G  LV+ + DATTN ++ TG  +S+ V LV +N D+ E + ++   +RNIL+ R+
Sbjct: 105 IEAEDGSPLVIELVDATTNTLVSTGPFSSSRVELVPLNADFTEESWTVEGFNRNILTQRE 164

Query: 180 GKRPLLVGNGIKLNLENGVAFIK-CVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGF 239
           GKRPLL G+ + + L+NGV  I   ++ +DNSSW +S+KFRL AK   +  +        
Sbjct: 165 GKRPLLTGD-LTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAVE------- 224

Query: 240 AVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDF 294
           A S++F   D RGE  KKHHPP   DEVWRLE I KDG     L+   I  V DF
Sbjct: 225 ARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDF 270

BLAST of Carg28138 vs. TAIR 10
Match: AT5G26920.2 (Cam-binding protein 60-like G )

HSP 1 Score: 159.8 bits (403), Expect = 4.1e-39
Identity = 95/235 (40.43%), Postives = 133/235 (56.60%), Query Frame = 0

Query: 60  LSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNE 119
           + N ++  V   +Q    P L S     E            + KL F N PPS+IFT ++
Sbjct: 45  MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSET-PSSRSRLKLCFINSPPSSIFTGSK 104

Query: 120 IKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSELSRNILSPRD 179
           I+AE+G  LV+ + DATTN ++ TG  +S+ V LV +N D+ E + ++   +RNIL+ R+
Sbjct: 105 IEAEDGSPLVIELVDATTNTLVSTGPFSSSRVELVPLNADFTEESWTVEGFNRNILTQRE 164

Query: 180 GKRPLLVGNGIKLNLENGVAFIK-CVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGF 239
           GKRPLL G+ + + L+NGV  I   ++ +DNSSW +S+KFRL AK   +  +        
Sbjct: 165 GKRPLLTGD-LTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAVE------- 224

Query: 240 AVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDF 294
           A S++F   D RGE  KKHHPP   DEVWRLE I KDG     L+   I  V DF
Sbjct: 225 ARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDF 270

BLAST of Carg28138 vs. TAIR 10
Match: AT5G62570.1 (Calmodulin binding protein-like )

HSP 1 Score: 150.6 bits (379), Expect = 2.5e-36
Identity = 89/243 (36.63%), Postives = 138/243 (56.79%), Query Frame = 0

Query: 56  IQPILSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIF 115
           ++P++   V+ EV  AL  H    ++ + E+     E+         +L F N     +F
Sbjct: 14  LEPLIRKVVKEEVELALGKHLAG-IKWICEKETHPLES------RNLQLKFLNNLSLPVF 73

Query: 116 TNNEIKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSE-LSRNI 175
           T+  I+ + G+A+ V + D +T  I  +G  +SA + + VV GD++  +    E +  NI
Sbjct: 74  TSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVEGDFNSVSDWTDEDIRNNI 133

Query: 176 LSPRDGKRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFP 235
           +  R+GK+PLL GN   + L +G+  +  +S TDNSSW +S+KFRL  + +D+     + 
Sbjct: 134 VREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---FDYV 193

Query: 236 TVGFAVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
            +  A+++SF V DHRGE+ KKHHPPS  DEVWRLE I KDG +HR L+   I  V DFL
Sbjct: 194 KIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVKDFL 245

Query: 296 LEY 298
             +
Sbjct: 254 THF 245

BLAST of Carg28138 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 150.6 bits (379), Expect = 2.5e-36
Identity = 89/243 (36.63%), Postives = 138/243 (56.79%), Query Frame = 0

Query: 56  IQPILSNWVQVEVMNALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIF 115
           ++P++   V+ EV  AL  H    ++ + E+     E+         +L F N     +F
Sbjct: 32  LEPLIRKVVKEEVELALGKHLAG-IKWICEKETHPLES------RNLQLKFLNNLSLPVF 91

Query: 116 TNNEIKAENGEALVVAICDATTNAIIRTGRLASALVGLVVVNGDYDETNSSLSE-LSRNI 175
           T+  I+ + G+A+ V + D +T  I  +G  +SA + + VV GD++  +    E +  NI
Sbjct: 92  TSARIEGDEGQAIRVGLIDPSTGQIFSSGPASSAKLEVFVVEGDFNSVSDWTDEDIRNNI 151

Query: 176 LSPRDGKRPLLVGNGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFP 235
           +  R+GK+PLL GN   + L +G+  +  +S TDNSSW +S+KFRL  + +D+     + 
Sbjct: 152 VREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQ---FDYV 211

Query: 236 TVGFAVSQSFRVMDHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
            +  A+++SF V DHRGE+ KKHHPPS  DEVWRLE I KDG +HR L+   I  V DFL
Sbjct: 212 KIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVKDFL 263

Query: 296 LEY 298
             +
Sbjct: 272 THF 263

BLAST of Carg28138 vs. TAIR 10
Match: AT2G18750.1 (Calmodulin-binding protein )

HSP 1 Score: 142.9 bits (359), Expect = 5.2e-34
Identity = 101/287 (35.19%), Postives = 154/287 (53.66%), Query Frame = 0

Query: 12  KAKMPPNDD-DHNPNPKRTRPSSSSGIGATSVAEDERYVLECLSRIQPILSNWVQVEVMN 71
           K K+  +D+      P+R RP+ +S I   ++  D   +    S ++PIL   V  EV  
Sbjct: 15  KRKLEEDDNQQQQQQPERKRPALASVI-VEALKMDS--LQRLCSSLEPILRRVVSEEVER 74

Query: 72  ALQTHFHPLLESLRERREGGEEAIFEGGIEKFKLVFCNQPPSTIFTNNEIKAENGEALVV 131
           AL       L   R       + I   G    +L F ++    +FT  +I+ E G A+ V
Sbjct: 75  ALAK-----LGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTGGKIEGEQGAAIHV 134

Query: 132 AICDATTNAIIRTGRLASALVGLVVVNGDY---DETNSSLSELSRNILSPRDGKRPLLVG 191
            + D TT  ++  G  ASA + +VV++GD+   D+   S  E   +++  R GKRPLL G
Sbjct: 135 VLLDMTTGHVLTVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGHLVKERQGKRPLLTG 194

Query: 192 NGIKLNLENGVAFIKCVSITDNSSWMKSKKFRLVAKFIDEKVLAMFPTVGFAVSQSFRVM 251
           + +++ L+ GV  +  +  TDNSSW++ +KFRL  +        M   V  A +++F V 
Sbjct: 195 D-VQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM--RVREAKTEAFTVK 254

Query: 252 DHRGEVNKKHHPPSREDEVWRLEGIRKDGPYHRNLSFHGIKNVDDFL 295
           DHRGE+ KKH+PP+ +DEVWRLE I KDG +H+ L+  GI NV +FL
Sbjct: 255 DHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 290