Carg28123 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg28123
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpumilio homolog 12-like
LocationCarg_Chr11: 3616449 .. 3621293 (+)
RNA-Seq ExpressionCarg28123
SyntenyCarg28123
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATATGGGTTCTGAACTGGAGATTGAGGACCTTGCAAGGCTTATTGGTGATGAGATTCCAAATGTTGCATCTGGTAATGCACATGCTGAAGAAAAGGGGTTGTTTTCGATACCTGTGGGTCCCTATGAAGAGACTTCCATTAGAAACTTCCATGGCAATGGGAATTTGATGGATGGAAATATCTCTATGAGTACACTCCAGCAATCCTCCATGGATCAAATGCTAACTAGGAAAGATACTATGCCAGGAGACCAGTCCTTGGCACTTGCATTTAGCAGTATCCATAAATATTATGAGGTTCCAATTGCTCGCCATCTCGAACAGTTGAATCGTGAACCATCTTGGGAGGCATGTGCATTTCATAGTGTTTCAAAGCAGTTAAATCCGGCATTTTCAACGCGCTATATGGACACAGTTCAAGGTATGGAGAAAGTTTCCTTTCCTAAAAAGATACTTGCTAGGAATACTCGACTGAACGCAGTTGATGCTATGAGGTTTATGTCTATTGGTGCTGATAAATCAGCCAATCATGTTGACCGAAGTAGAATTGTCTGCTCAAATAGCTATGTTCGGCACAACAATTTTGCTCCAGCTACTGAACGCATTTTTCGTAATGAACTAAGCTCATCTGCTTTGTACTTGGAATCTGCAAATCTCAAGTTAGTGCCTGAAAGATTTAACTCTGTTGATGAAGTCAGAGGTAAAATATTTCTAATGGCAAAAGACCAACATGGTTGCCGTTTCTTGCAACGGAAATTTATGGAGGGTACTGATGAAGATATTGAGAAGATTTTCAATGAAATCATTGATCATGTTGTTGACCTTATGGTAGATCCTTTTGGAAACTATCTAGTACAGAAGCTGCTTGAAGTTTGCAATGAAGATCAGCGGATGAAATTTCTACGTAGAATTACACAGAACCATGGTGAAATTATTCTAGTCTCATGTGACATGCATGGGTATGCTCTTTTCCTTTCATTTTCTATGCCTCAATGGCCGAACACAAGAATTTATGGACTTCATTGCATTTTTTTTTAATGTTTTTAATTTTTCTTCCAAATTCATTTTTCTCAATCACAGGACTCGTGCTGTTCAGAAGGTTATTGAGACTCTTAAAACCCAAGAGCAAGTTTTCATGATTGTCTCGTCTTTGAAGTCTGGTATGGTGACTCTGATGAAAAACGTAAACGGAAACCATGTGGCACAACATTGCCTTGATTATTTAATGCCTAGCAGTGAGGTCAGGATTATATTTTCTACCTCTGTTTCTTTGAATTTGTTCGTTTCAAGTGAGAATGTGCTTGCCTTTTCATCTGTAGTTTAGAACTACAAAAAGGTGCTTACCTTTTTTTATTTGATCTCTTTCCCTTTTAGTCCATTTTTCTGGGGAGTTTTGCTATTTTTCATTCAAATTGTTCCTTTGGTTATGCCCCATTCAAGTGATAGCAAGTCTCCAAAAGTATTTGATAGGCCTTTTACATAGTTTACCTATGAGAGGAGAATGTATGGAGGCCATGGATAATTTCTGGTAGCCAAGGTGGCACCCTTAGGATTAAGATATTTTGTCAGTAACTGCAGCAAGTAGTTTTTTTTTTTTTTTTTGAGGAAATAGTTCGAAGGGTAGAATGACAAGAAGCAAGAAATTTCGAAGAAGTCTGTATATGAATATGAGGAAAGGATTTAATTGAAAATCTGAGTGCACATGTCCCAGCTTTTTACTTCCTATCAAATTTTTGTTTCTCCCAAAATTTTCTTAGTCTTTTCCCAGTAACAATTAATATATCCACATCTTTAAATGTGCTGAGAAAATTGTCATGTATATGGTTTTATAGACTCAGGTGAGTGGAACATTGAAGAAAGCGAGGTTAAGAGGTTTTGATAAGTTAAACACTGGAGTTTTCAATGATGCTTGAATGACTACTTTAGACTTGTTTTTGCATCTGTGTTTCTAAATCTGCTAGAAAATTACTCTCTCAAGTACTTGTGCTCCCTCCCTCCTTCCTTAAAAAGTGAGACGTGACAACACGACAAGCAGTTTATTCTCTCTCTATAATTTCTTTGTCAATGTTTTCCGTAGATCCTTATATTGTTCGTTTGGAAGTTATATGGATTAATTGGTAAAGTAAGGAGTTATATCAATCACTAACAATCTTTTTAGACTTGAGCCTGCTGTCTTTTCAGTTTGACTGTTGTTACTGAATATAATACCATTTTTAGCCATGTTGATATTGATGGCTGAACTTTTTTTTTTTTTTTGGTCCAAATTTGGTGGAAGTAGATATGATTTGCATGGTTGTGATTCTTTGATCTTTTAATCAGGCGTGTGTAACCCCCCTCCTCCTCCTCCTCAACTGGTCTTTGACATTGAGGAAGATTCATTTTGTAACATGAATTCTGTGAAACAAATAATTAATTCTGGGGTTATCACGTTCACTGCAGGTACTGTTTGATGCTGTAAGAAATAGTTGTGTTGATCTTGCGACTGATCGTCATGGTTGTTGTGTGCTTCAAAAATACCTCACTTGTTCCAATAGCGTACTCCGAGATAATTTAATCAGTGAGATTGCTCACAATGCGTTGGTTATTTCCCAAGATCAATATGGGTAATTAACTCTTTCTTTCTTTTTTTCTTTTCTTTTTTCTCTAACCTTTTCTTTAATATGCTTGCTCACAATGCGTTGGATATATCTTTGTTTCCTTAATTTTTATTCCAAAACCCTTTTCTTCATTTGATCCAAAACGATTCTGAATTTTAAAACTTATTTTCTTTACGTAGCCACAGGATCATGTTGGACCCTTTTAGAATTTCCAAATTTCTGAATTTAATCGGTCTCTTTTCTAATGTCTTTTCTCATTTGTATTTGACTACTTCGAGAGCATTTTGTTTTCTTTTTTTGCTTTCGGATAACTGAATAATTCATGTGAGATAAACTGCTTTGTAGATGCTTGGATAATTTCAACTTGTTACCAATCCGGTGGGTCATGGTCCTTCTTATGCCTTGCTCATGGCTGCATGGCCATACCAACCTTTTTATTCATTTTTCATTATATGTCTTCTTTTGTATGTTCATTTTATTTCCTTTTACTTTTGTAAGCGTATGACTTTTAATATGCAAGTTTACCATAGACAATTTGCCTCATAATATACGATAGGAGATACAACAAATATCAAATTTCCTATCTAGGCTCTATGTTATCAATGCTTTTCTGGTTCCTTATTGCTTGTTAGTGCATAAAATTGGCTATCTCAGAATGCTTTCCAAAACGTTCTCTAAAAACATTTTCTAACCCTGTTTTTTAAAGCCGTTTCAGTTGGTTAACTTATTCAAGGGCTTGAGTAAGTTCTGCACAATCGCTTTATAAGTCTCGAAGAAGGGTGATTGTGACATTGTGAACCCCAAAACGTATACAAAATTCGTAATTTTTCTGACAATGGCTGCATTCTGGAGATGTATATATTCAACTCCAATTCCAATATGAATATTGGAGTTGACTTGGGTCTATGGATTAGAACTGACAAGTTATATTTCACTAAATTATTTATATGCAGGAACTATGTTGTGCAATTCATCCTAAGGTTGAAGCGTCGATGGTATACGGAAGCTATAGTCAAACAGTTAGAGGGTAGTTTTGGTGACTTGGCCATGCAGAAGTATAGTAGTAATGTTGTTGAAAAATGCCTTGAATCTGCAAGGGAATGCGGGAGATATCTCTAAAAATTGTGCTTGAATTGGATCAATGATCTGCGATGATAAAATCATGCAGGACCCATATGCAATTTGTCAATTCAAACTGCATTGGTAGTTACAAGGTTAGCAGGGCAATATTTTCCTTCTCTTTTGGTATCTGTGTTTATATGTATGAGGAATGTGTGAAAGTCTAGGTTGTTAACTTTTTTGTGTAAGGAACGTAGTACTGCTTTAACACCTTTGGCATCTTCGACACCTTCTGTTGTCGAATGGTGCCTTTAACTTTTTCAAATTTATATCTTGAAAAGTTAGAACAAAAGTCAGACATCATGAAAAACACTACACATCCCATGTTGCTATGTCATACCAACTACAATCTTGAGAATTTTGCTGATCAGGTTACAGAAAAGCCCTCCAACTATAACTGAGTTTCTTTCCGACCAAAAATGTACGCCACATGACCGTTGTAATCATGACGTAATTATGATCTCAGTCTTGTTCTTTTAGCATAGAGTCCTTTTTTGCAGTTAGTTTTGGACGCCTCCTTTTCTACTCGAAGGTCATAACACTTTGGTCTATGGAAATTTGAATGCAAAATGTCGACTTTTCTATTTCAAATGAAGGTTTATGGTAGCGTTAGTGTTTACATTTATAGGTGATTGTCGAACTGATTTCTAATGCAATTAGATCAATGTTTTTCGGCTGCAGGGTGCTCTTCATACTAAACTCGTGGAAGCCATTAAGCTGCATGCGCCTGTACTTAGGACAAGTCCATACGGAAAGAAAGTCCTTGTGGTACTTGGAAAATCAAACTAACTGGTTACAAACAAACGAGAGCGCTTCGTCTAGTCTTTGTTTCAACTTGGTAAGAAAGCACGACTAATATTTGTTTTCCTTCAGTACTTTTTTGTAAAGAGTTTAGGCAGAGGTCTATCTGGAAAGAAAAACTTGTCAAAGGTGAAGACGTATCGAACTCTTCACTCCTAATTTTCGTTTCGGGTAAAGGTTTAGGATGCTTAAACGATGGCTGAGGTCTAGCTGGCCATGTTGTTTTTGTCGTTTGTTTATTTATTTATTTATTTATTCCGTTTATTGGACATAGTAAATAAAGAGGTGGTGAGGGTATAGAGAAGGGGTCATGGAGTGTTGT

mRNA sequence

ATGGAAGATATGGGTTCTGAACTGGAGATTGAGGACCTTGCAAGGCTTATTGGTGATGAGATTCCAAATGTTGCATCTGGTAATGCACATGCTGAAGAAAAGGGGTTGTTTTCGATACCTGTGGGTCCCTATGAAGAGACTTCCATTAGAAACTTCCATGGCAATGGGAATTTGATGGATGGAAATATCTCTATGAGTACACTCCAGCAATCCTCCATGGATCAAATGCTAACTAGGAAAGATACTATGCCAGGAGACCAGTCCTTGGCACTTGCATTTAGCAGTATCCATAAATATTATGAGGTTCCAATTGCTCGCCATCTCGAACAGTTGAATCGTGAACCATCTTGGGAGGCATGTGCATTTCATAGTGTTTCAAAGCAGTTAAATCCGGCATTTTCAACGCGCTATATGGACACAGTTCAAGGTATGGAGAAAGTTTCCTTTCCTAAAAAGATACTTGCTAGGAATACTCGACTGAACGCAGTTGATGCTATGAGGTTTATGTCTATTGGTGCTGATAAATCAGCCAATCATGTTGACCGAAGTAGAATTGTCTGCTCAAATAGCTATGTTCGGCACAACAATTTTGCTCCAGCTACTGAACGCATTTTTCGTAATGAACTAAGCTCATCTGCTTTGTACTTGGAATCTGCAAATCTCAAGTTAGTGCCTGAAAGATTTAACTCTGTTGATGAAGTCAGAGGTAAAATATTTCTAATGGCAAAAGACCAACATGGTTGCCGTTTCTTGCAACGGAAATTTATGGAGGGTACTGATGAAGATATTGAGAAGATTTTCAATGAAATCATTGATCATGTTGTTGACCTTATGGTAGATCCTTTTGGAAACTATCTAGTACAGAAGCTGCTTGAAGTTTGCAATGAAGATCAGCGGATGAAATTTCTACGTAGAATTACACAGAACCATGGTGAAATTATTCTAGTCTCATGTGACATGCATGGGACTCGTGCTGTTCAGAAGGTTATTGAGACTCTTAAAACCCAAGAGCAAGTTTTCATGATTGTCTCGTCTTTGAAGTCTGGTATGGTGACTCTGATGAAAAACGTAAACGGAAACCATGTGGCACAACATTGCCTTGATTATTTAATGCCTAGCAGTGAGGTACTGTTTGATGCTGTAAGAAATAGTTGTGTTGATCTTGCGACTGATCGTCATGGTTGTTGTGTGCTTCAAAAATACCTCACTTGTTCCAATAGCGTACTCCGAGATAATTTAATCAGTGAGATTGCTCACAATGCGTTGGTTATTTCCCAAGATCAATATGGGAACTATGTTGTGCAATTCATCCTAAGGTTGAAGCGTCGATGGTATACGGAAGCTATAGTCAAACAGTTAGAGGGTAGTTTTGGTGACTTGGCCATGCAGAAGTATAGTAGTAATGTTGTTGAAAAATGCCTTGAATCTGCAAGGGAATGCGGGAGATATCTCTAAAAATTGTGCTTGAATTGGATCAATGATCTGCGATGATAAAATCATGCAGGACCCATATGCAATTTGTCAATTCAAACTGCATTGGTAGTTACAAGGGTGCTCTTCATACTAAACTCGTGGAAGCCATTAAGCTGCATGCGCCTGTACTTAGGACAAGTCCATACGGAAAGAAAGTCCTTGTGGTACTTGGAAAATCAAACTAACTGGTTACAAACAAACGAGAGCGCTTCGTCTAGTCTTTGTTTCAACTTGGTAAGAAAGCACGACTAATATTTGTTTTCCTTCAGTACTTTTTTGTAAAGAGTTTAGGCAGAGGTCTATCTGGAAAGAAAAACTTGTCAAAGGTGAAGACGTATCGAACTCTTCACTCCTAATTTTCGTTTCGGGTAAAGGTTTAGGATGCTTAAACGATGGCTGAGGTCTAGCTGGCCATGTTGTTTTTGTCGTTTGTTTATTTATTTATTTATTTATTCCGTTTATTGGACATAGTAAATAAAGAGGTGGTGAGGGTATAGAGAAGGGGTCATGGAGTGTTGT

Coding sequence (CDS)

ATGGAAGATATGGGTTCTGAACTGGAGATTGAGGACCTTGCAAGGCTTATTGGTGATGAGATTCCAAATGTTGCATCTGGTAATGCACATGCTGAAGAAAAGGGGTTGTTTTCGATACCTGTGGGTCCCTATGAAGAGACTTCCATTAGAAACTTCCATGGCAATGGGAATTTGATGGATGGAAATATCTCTATGAGTACACTCCAGCAATCCTCCATGGATCAAATGCTAACTAGGAAAGATACTATGCCAGGAGACCAGTCCTTGGCACTTGCATTTAGCAGTATCCATAAATATTATGAGGTTCCAATTGCTCGCCATCTCGAACAGTTGAATCGTGAACCATCTTGGGAGGCATGTGCATTTCATAGTGTTTCAAAGCAGTTAAATCCGGCATTTTCAACGCGCTATATGGACACAGTTCAAGGTATGGAGAAAGTTTCCTTTCCTAAAAAGATACTTGCTAGGAATACTCGACTGAACGCAGTTGATGCTATGAGGTTTATGTCTATTGGTGCTGATAAATCAGCCAATCATGTTGACCGAAGTAGAATTGTCTGCTCAAATAGCTATGTTCGGCACAACAATTTTGCTCCAGCTACTGAACGCATTTTTCGTAATGAACTAAGCTCATCTGCTTTGTACTTGGAATCTGCAAATCTCAAGTTAGTGCCTGAAAGATTTAACTCTGTTGATGAAGTCAGAGGTAAAATATTTCTAATGGCAAAAGACCAACATGGTTGCCGTTTCTTGCAACGGAAATTTATGGAGGGTACTGATGAAGATATTGAGAAGATTTTCAATGAAATCATTGATCATGTTGTTGACCTTATGGTAGATCCTTTTGGAAACTATCTAGTACAGAAGCTGCTTGAAGTTTGCAATGAAGATCAGCGGATGAAATTTCTACGTAGAATTACACAGAACCATGGTGAAATTATTCTAGTCTCATGTGACATGCATGGGACTCGTGCTGTTCAGAAGGTTATTGAGACTCTTAAAACCCAAGAGCAAGTTTTCATGATTGTCTCGTCTTTGAAGTCTGGTATGGTGACTCTGATGAAAAACGTAAACGGAAACCATGTGGCACAACATTGCCTTGATTATTTAATGCCTAGCAGTGAGGTACTGTTTGATGCTGTAAGAAATAGTTGTGTTGATCTTGCGACTGATCGTCATGGTTGTTGTGTGCTTCAAAAATACCTCACTTGTTCCAATAGCGTACTCCGAGATAATTTAATCAGTGAGATTGCTCACAATGCGTTGGTTATTTCCCAAGATCAATATGGGAACTATGTTGTGCAATTCATCCTAAGGTTGAAGCGTCGATGGTATACGGAAGCTATAGTCAAACAGTTAGAGGGTAGTTTTGGTGACTTGGCCATGCAGAAGTATAGTAGTAATGTTGTTGAAAAATGCCTTGAATCTGCAAGGGAATGCGGGAGATATCTCTAA

Protein sequence

MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMDGNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIHKYYEVPIARHLEQLNREPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQHCLDYLMPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESARECGRYL
Homology
BLAST of Carg28123 vs. NCBI nr
Match: KAG7022014.1 (Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 966.5 bits (2497), Expect = 8.8e-278
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD
Sbjct: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIHKYYEVPIARHLEQLNREPSWEAC 120
           GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIHKYYEVPIARHLEQLNREPSWEAC
Sbjct: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIHKYYEVPIARHLEQLNREPSWEAC 120

Query: 121 AFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHV 180
           AFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHV
Sbjct: 121 AFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHV 180

Query: 181 DRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIFL 240
           DRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIFL
Sbjct: 181 DRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIFL 240

Query: 241 MAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRM 300
           MAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRM
Sbjct: 241 MAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRM 300

Query: 301 KFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGN 360
           KFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGN
Sbjct: 301 KFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGN 360

Query: 361 HVAQHCLDYLMPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHN 420
           HVAQHCLDYLMPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHN
Sbjct: 361 HVAQHCLDYLMPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHN 420

Query: 421 ALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESAREC 480
           ALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESAREC
Sbjct: 421 ALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESAREC 480

Query: 481 GRYL 485
           GRYL
Sbjct: 481 GRYL 484

BLAST of Carg28123 vs. NCBI nr
Match: XP_022925804.1 (pumilio homolog 12-like [Cucurbita moschata] >XP_022925813.1 pumilio homolog 12-like [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 9.2e-251
Identity = 477/669 (71.30%), Postives = 479/669 (71.60%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD
Sbjct: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAF  ++                       
Sbjct: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFEKLNFGDEAKGRTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNMDTSAFGALDSSNHLINGVHDPHVIRLGHQNSPSRHIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQSHVQYIHPQQINHQQMGWNY 240

Query: 241 ---------------------------------------------KYYEVPIARHLEQLN 300
                                                        KYYEVPIARHLEQLN
Sbjct: 241 TEEEQLYRMHEQYPYPQHLYIQQRWLPNQSHENHSSRISCQSGKQKYYEVPIARHLEQLN 300

Query: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360
           REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG
Sbjct: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360

Query: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420
           ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD
Sbjct: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420

Query: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480
           EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE
Sbjct: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480

Query: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 482
           VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT
Sbjct: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 540

BLAST of Carg28123 vs. NCBI nr
Match: XP_023529396.1 (pumilio homolog 12-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529397.1 pumilio homolog 12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871.3 bits (2250), Expect = 3.9e-249
Identity = 473/662 (71.45%), Postives = 477/662 (72.05%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD
Sbjct: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GN+SMSTLQQSSMDQMLTRKD MPGDQSLALAF  ++                       
Sbjct: 61  GNVSMSTLQQSSMDQMLTRKDAMPGDQSLALAFEKLNFGGEAKGHTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNMDTSALGALDSSNHLINGVHDPHVIRLGHQNSPSRLIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQSHVQYIHPQQINRQQMGWNY 240

Query: 241 --------------------------------------KYYEVPIARHLEQLNREPSWEA 300
                                                 KYYEVPIARHLEQLNREPSWEA
Sbjct: 241 TEEEQYPYPQHLYIQQRWLPNQSHENHSSRISCQSGKQKYYEVPIARHLEQLNREPSWEA 300

Query: 301 CAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANH 360
           CAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANH
Sbjct: 301 CAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANH 360

Query: 361 VDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIF 420
           VDRSRIV SNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIF
Sbjct: 361 VDRSRIVRSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIF 420

Query: 421 LMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQR 480
           LMAKDQHGCRFLQRKFMEGTD+DIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQR
Sbjct: 421 LMAKDQHGCRFLQRKFMEGTDDDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQR 480

Query: 481 MKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNG 482
           MKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNG
Sbjct: 481 MKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNG 540

BLAST of Carg28123 vs. NCBI nr
Match: XP_022966848.1 (pumilio homolog 12-like [Cucurbita maxima] >XP_022966857.1 pumilio homolog 12-like [Cucurbita maxima])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 467/669 (69.81%), Postives = 473/669 (70.70%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMG+ELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFH NGNLMD
Sbjct: 1   MEDMGTELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHRNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GNISM+TLQQSSMDQMLTRKD MPGD+SLALAF  ++                       
Sbjct: 61  GNISMNTLQQSSMDQMLTRKDAMPGDRSLALAFEKLNFGDEAKGRTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNVDASALGALDSSNHLINGVHDPHMIRLGHQNSPSRHIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQSHVQYIHPQQINRQQMGWNY 240

Query: 241 ---------------------------------------------KYYEVPIARHLEQLN 300
                                                        KYYEVPIARHLEQLN
Sbjct: 241 TEEEQLYRMHEQYPYPQHLYIQQRWLPNQSQENHSSRTSCQGGKQKYYEVPIARHLEQLN 300

Query: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360
           RE SWEACAFHSVSK LNPAFSTRY+DTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG
Sbjct: 301 REASWEACAFHSVSKHLNPAFSTRYIDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360

Query: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420
           ADKSANHVDRSRIV SNS VRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD
Sbjct: 361 ADKSANHVDRSRIVRSNSCVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420

Query: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480
           EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE
Sbjct: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480

Query: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 482
           VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT
Sbjct: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 540

BLAST of Carg28123 vs. NCBI nr
Match: KAG7022006.1 (Pumilio-like 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 847.8 bits (2189), Expect = 4.6e-242
Identity = 468/669 (69.96%), Postives = 470/669 (70.25%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD
Sbjct: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GNISMSTLQQSSMDQML        DQSLALAF  ++                       
Sbjct: 61  GNISMSTLQQSSMDQMLR-------DQSLALAFEKLNFGDEAKGRTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNMDTSAFGALDSSNHLINGVHDPHVIRLGHQNSPSRHIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQPHVQYIHPQQINHQQMGWNY 240

Query: 241 ---------------------------------------------KYYEVPIARHLEQLN 300
                                                        KYYEVPIARHLEQLN
Sbjct: 241 TEEEQLYRMHEQYPYPQHLYIQQRWLPNQSHENHSSRISCQSGKQKYYEVPIARHLEQLN 300

Query: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360
           REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG
Sbjct: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360

Query: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420
           ADKSANHVDRSRIV SNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD
Sbjct: 361 ADKSANHVDRSRIVRSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420

Query: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480
           EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE
Sbjct: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480

Query: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 482
           VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT
Sbjct: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 540

BLAST of Carg28123 vs. ExPASy Swiss-Prot
Match: Q9LVC3 (Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2)

HSP 1 Score: 328.2 bits (840), Expect = 1.6e-88
Identity = 160/273 (58.61%), Postives = 211/273 (77.29%), Query Frame = 0

Query: 206 RNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEK 265
           +N+L+   L +   NL L P+++NS+ E RGKI+ +AKDQHGCRFLQR F E    DIE 
Sbjct: 251 QNQLNED-LTMSLNNLSLQPQKYNSIAEARGKIYYLAKDQHGCRFLQRIFSEKDGNDIEM 310

Query: 266 IFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRA 325
           IFNEIID++ +LM+DPFGNYLVQKLLEVCNEDQRM+ +  IT+  G +I +SCDMHGTRA
Sbjct: 311 IFNEIIDYISELMMDPFGNYLVQKLLEVCNEDQRMQIVHSITRKPGLLIKISCDMHGTRA 370

Query: 326 VQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQHCLDYLMP-SSEVLFDAVRNS 385
           VQK++ET K +E++ +I+S+LK G+V L+KNVNGNHV Q CL YL+P   + LF+A    
Sbjct: 371 VQKIVETAKREEEISIIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAAITH 430

Query: 386 CVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRW 445
           CV+LATDRHGCCVLQK L  S    + +L+SEIA NAL++SQD +GNYV+Q++  L+ +W
Sbjct: 431 CVELATDRHGCCVLQKCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFELQLQW 490

Query: 446 YTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESA 478
            T  I++QLEG++ +L+MQK SSNVVEKCL+ A
Sbjct: 491 ATFEILEQLEGNYTELSMQKCSSNVVEKCLKLA 522

BLAST of Carg28123 vs. ExPASy Swiss-Prot
Match: Q9LM20 (Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM8 PE=3 SV=2)

HSP 1 Score: 255.4 bits (651), Expect = 1.3e-66
Identity = 119/255 (46.67%), Postives = 183/255 (71.76%), Query Frame = 0

Query: 226 ERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNY 285
           E    V E +G ++ MAKDQHGCRFLQ  F +G+  D   IF+E+I HVV+LM+DPFGNY
Sbjct: 191 ESLPKVSEFQGYVYFMAKDQHGCRFLQWIFEDGSALDALVIFSEVIPHVVELMMDPFGNY 250

Query: 286 LVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSS 345
           L+QKLL+VCNE+QR + +  +T   G++I +S + +GTR VQ+++E++KT++Q+ ++ S+
Sbjct: 251 LMQKLLDVCNEEQRTQIILMVTSEPGQLIRISLNAYGTRVVQRLVESIKTRKQISLVKSA 310

Query: 346 LKSGMVTLMKNVNGNHVAQHCLDYL-MPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTC 405
           L+ G + L++++NGNHV Q CL  L    +E +F+     C+D+AT RHGCCVLQK +  
Sbjct: 311 LRPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDATKFCIDIATHRHGCCVLQKCIAY 370

Query: 406 SNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQK 465
           S+ + R+ L++EI+ N+L ++QD YGNY VQF+L L+      A++ QL+G + +L+MQK
Sbjct: 371 SSGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLELRDFSAIAAMLAQLKGHYVELSMQK 430

Query: 466 YSSNVVEKCLESARE 480
           +SS++VE+CL    E
Sbjct: 431 FSSHMVERCLTHCPE 445

BLAST of Carg28123 vs. ExPASy Swiss-Prot
Match: Q9C9R6 (Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM7 PE=3 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 9.4e-65
Identity = 123/290 (42.41%), Postives = 194/290 (66.90%), Query Frame = 0

Query: 187 CSNSYVRHNNFAPATERIFRNELSSSALYLESAN-LKLVPERFNSVDEVRGKIFLMAKDQ 246
           CS  +    +F    + I     ++ AL   + N  ++      ++ +++G ++LMAKDQ
Sbjct: 286 CSEPFSSDESFFMNGKSIDHQRSNTRALMSNNGNPTEICHPSLPNMCDIQGYVYLMAKDQ 345

Query: 247 HGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRR 306
           HGCRFLQR F EGT  D   IFNE+I HVV+LM+DPFGNYL+QKLL+VC E+QR + +  
Sbjct: 346 HGCRFLQRIFDEGTSVDAMIIFNEVIAHVVELMMDPFGNYLMQKLLDVCTEEQRTQIVLV 405

Query: 307 ITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQH 366
            T+  G++I +S + +GTR VQ+++ET+++ +Q+ ++  +L+ G + L+K++NGNHV Q 
Sbjct: 406 ATEEPGQLIRISLNAYGTRVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQR 465

Query: 367 CLDYL-MPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVI 426
           CL  L    ++ +FDA    C ++AT RHGCCVLQK +  S    R+ LI+EI+ N+L++
Sbjct: 466 CLQCLSTEDNKFIFDAATKFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLL 525

Query: 427 SQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCL 475
           +QD +GNY VQF++ L+       ++ QL+G +  L+MQK+SS++VE+CL
Sbjct: 526 AQDPFGNYAVQFVIELRIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCL 575

BLAST of Carg28123 vs. ExPASy Swiss-Prot
Match: Q9LDW3 (Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 4.2e-57
Identity = 107/240 (44.58%), Postives = 168/240 (70.00%), Query Frame = 0

Query: 236 GKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCN 295
           G ++LMAKDQ GCR LQ+   EG   D+  IF E+I++V++L  DPFGNYL+QKL+EVCN
Sbjct: 244 GSVYLMAKDQLGCRLLQKFVDEGNFVDVMIIFKEVINNVIELGTDPFGNYLIQKLIEVCN 303

Query: 296 EDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMK 355
           E+QR + L R+T   G ++ +S + +GTR VQK+IET+ T+EQ+ ++ S+L  G ++L +
Sbjct: 304 EEQRTQILIRLTSKPGLLVKISINNYGTRVVQKLIETVTTKEQISLVKSALVPGFLSLFR 363

Query: 356 NVNGNHVAQHCLDYLMPS-SEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLI 415
            +NGNHV  +CL +  P+ ++ + +A    C+++AT RHGCCVLQ+ ++ S     + L+
Sbjct: 364 ELNGNHVILNCLKFFSPNDNKFILEAATKFCIEIATTRHGCCVLQRCVSYSVGEQHEKLV 423

Query: 416 SEIAHNALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCL 475
            EI+ N+L+++QD +GNY+VQ+I+  K       ++ +L G++  LA QK+ S+VVEKCL
Sbjct: 424 DEISRNSLLLAQDPFGNYLVQYIIEKKVGGVN--VLFELRGNYVKLATQKFGSHVVEKCL 481

BLAST of Carg28123 vs. ExPASy Swiss-Prot
Match: Q1PFN9 (Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 6.1e-56
Identity = 117/275 (42.55%), Postives = 179/275 (65.09%), Query Frame = 0

Query: 209 LSSSALYLESANLKLVPERFN---SVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEK 268
           L  + L  + +  K+ P+  N   S+ E+ G + LMAKDQ GCR LQ+   EGT  + + 
Sbjct: 222 LEDTVLIGQGSYGKMSPKSNNDLVSMVEIYGSVNLMAKDQIGCRVLQKLVEEGTFHEAKV 281

Query: 269 IFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRA 328
           I   IIDHVV+L +DPFGNY+VQKL +V +E+QR   +  +T N  E+I +  + +GTR 
Sbjct: 282 ILLAIIDHVVELSMDPFGNYIVQKLFDVSDEEQRTLIVSVLTSNPRELIRICLNTYGTRV 341

Query: 329 VQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQHCLDYLMPS-SEVLFDAVRNS 388
           VQK+IET+KT++Q+ ++ S LK G + L+K++NGNHV Q CL  L P+ +E + +A    
Sbjct: 342 VQKMIETVKTKQQIALVKSGLKPGFLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAATKY 401

Query: 389 CVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRW 448
           C ++A  RHGCCVLQ  ++ S  + R+ L++EI+ N+L +SQD +GNYVVQ++  + ++ 
Sbjct: 402 CAEIAIHRHGCCVLQCCISNSVGLQRERLVAEISRNSLHLSQDPFGNYVVQYL--IDQQV 461

Query: 449 YTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESARE 480
               ++ Q    + +LA QK+SS+V+EKCL    E
Sbjct: 462 SAVKLLVQFRMHYAELATQKFSSHVIEKCLRKYPE 494

BLAST of Carg28123 vs. ExPASy TrEMBL
Match: A0A6J1EGB2 (pumilio homolog 12-like OS=Cucurbita moschata OX=3662 GN=LOC111433100 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.5e-251
Identity = 477/669 (71.30%), Postives = 479/669 (71.60%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD
Sbjct: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAF  ++                       
Sbjct: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFEKLNFGDEAKGRTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNMDTSAFGALDSSNHLINGVHDPHVIRLGHQNSPSRHIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQSHVQYIHPQQINHQQMGWNY 240

Query: 241 ---------------------------------------------KYYEVPIARHLEQLN 300
                                                        KYYEVPIARHLEQLN
Sbjct: 241 TEEEQLYRMHEQYPYPQHLYIQQRWLPNQSHENHSSRISCQSGKQKYYEVPIARHLEQLN 300

Query: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360
           REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG
Sbjct: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360

Query: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420
           ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD
Sbjct: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420

Query: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480
           EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE
Sbjct: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480

Query: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 482
           VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT
Sbjct: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 540

BLAST of Carg28123 vs. ExPASy TrEMBL
Match: A0A6J1HP57 (pumilio homolog 12-like OS=Cucurbita maxima OX=3661 GN=LOC111466429 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.3e-244
Identity = 467/669 (69.81%), Postives = 473/669 (70.70%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           MEDMG+ELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFH NGNLMD
Sbjct: 1   MEDMGTELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHRNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSSIH----------------------- 120
           GNISM+TLQQSSMDQMLTRKD MPGD+SLALAF  ++                       
Sbjct: 61  GNISMNTLQQSSMDQMLTRKDAMPGDRSLALAFEKLNFGDEAKGRTWKSLQGHAIRLEDH 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 YSDNLIKQLVNVDASALGALDSSNHLINGVHDPHMIRLGHQNSPSRHIPDQYKKWQAGQL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 QPLETSSPAMPLTYEVANVSAQNLQFPITSQRQQIIHNGQSHVQYIHPQQINRQQMGWNY 240

Query: 241 ---------------------------------------------KYYEVPIARHLEQLN 300
                                                        KYYEVPIARHLEQLN
Sbjct: 241 TEEEQLYRMHEQYPYPQHLYIQQRWLPNQSQENHSSRTSCQGGKQKYYEVPIARHLEQLN 300

Query: 301 REPSWEACAFHSVSKQLNPAFSTRYMDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360
           RE SWEACAFHSVSK LNPAFSTRY+DTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG
Sbjct: 301 REASWEACAFHSVSKHLNPAFSTRYIDTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIG 360

Query: 361 ADKSANHVDRSRIVCSNSYVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420
           ADKSANHVDRSRIV SNS VRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD
Sbjct: 361 ADKSANHVDRSRIVRSNSCVRHNNFAPATERIFRNELSSSALYLESANLKLVPERFNSVD 420

Query: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480
           EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE
Sbjct: 421 EVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLE 480

Query: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 482
           VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT
Sbjct: 481 VCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVT 540

BLAST of Carg28123 vs. ExPASy TrEMBL
Match: A0A6J1JJB1 (pumilio homolog 12-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484953 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.0e-183
Identity = 363/631 (57.53%), Postives = 408/631 (64.66%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           ME+MG+ELE  DLARLIGDE PNVASGNAH EE+GLFS+PV  YE  S+RNF GNGNLMD
Sbjct: 1   MEEMGNELEFADLARLIGDEFPNVASGNAHNEEQGLFSVPVSSYEGPSVRNFDGNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSS------------------------- 120
           GNISMS  QQ      L+R+++MPGD SL LAF                           
Sbjct: 61  GNISMSKFQQ------LSRQNSMPGDSSLTLAFEKLNFGDEVKGRAWKSLQQGHAVLLED 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HYSENLNKQLHIMDPSVLASWGSSNHLSNGVHNPHMTKFSHQNSPSRHIPEQYNKWQFPI 180

Query: 181 --------------------------------------------------IHK------- 240
                                                             +HK       
Sbjct: 181 TPQMQQIFHDGRPPVRYIHPQQINHCQIGWNYTEEDKLYRMHEQYPYLQHLHKHQIQSHE 240

Query: 241 --------------YYEVPIARHLEQLNREPSWEACAFHSVSKQLNPAFSTRYMDTVQGM 300
                         YYEVPIA HLEQ N EPSW+  AF   S Q +P F + YMDT+QGM
Sbjct: 241 NIASRISCQNRKQPYYEVPIAHHLEQSNYEPSWKTYAFQRGSNQFSPVFPSHYMDTIQGM 300

Query: 301 EKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHVDRSRIVCSNSYVRHNNFAPATERI 360
           EK SFP+KILAR+ RLN VD+MRFMSIGADKSAN V++SRI+ SNS VR NN    TE I
Sbjct: 301 EKFSFPRKILARSPRLNTVDSMRFMSIGADKSANLVNQSRIIRSNSCVRRNNL---TECI 360

Query: 361 FRNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIE 420
           +R+   SSA+Y +SANLKL PERFNSVDEVRG+IFLMAKDQHGCRFLQRKF+EGTDEDIE
Sbjct: 361 YRDH--SSAMYSKSANLKLFPERFNSVDEVRGRIFLMAKDQHGCRFLQRKFVEGTDEDIE 420

Query: 421 KIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTR 475
           KIFNEIIDHVVDLMVD FGNYLVQKLLEVCNE+QRM+ L RITQNHGE++L+SCDMHGTR
Sbjct: 421 KIFNEIIDHVVDLMVDAFGNYLVQKLLEVCNENQRMQILHRITQNHGELVLISCDMHGTR 480

BLAST of Carg28123 vs. ExPASy TrEMBL
Match: A0A6J1H9W2 (pumilio homolog 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461817 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.0e-183
Identity = 361/631 (57.21%), Postives = 410/631 (64.98%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           ME+MG+ELE  DLARLIGDE PNVASGNAH EE+GLFS+PV  YE  S++NF  NGNLMD
Sbjct: 1   MEEMGNELEFADLARLIGDEFPNVASGNAHNEEQGLFSVPVSSYEGPSVQNFDSNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAFSS------------------------- 120
           GNISMS LQQ      L+R+++MPGD SL LAF                           
Sbjct: 61  GNISMSKLQQ------LSRQNSMPGDSSLTLAFEKLNFGDEAKGRAWKSLQQGHAVLLED 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HYSENLNKQLHIMDPSVLASWGSSNHLSNGVHSPHMTKFSHQNSPSRHIPEQYNNWQFPT 180

Query: 181 --------------------------------------------------IHK------- 240
                                                             +HK       
Sbjct: 181 TPQMQQIFHNGRPPVRYIHPQQINHCQIGWNYNEEDKLYRMHEQYPYLQHLHKQQIQSHE 240

Query: 241 --------------YYEVPIARHLEQLNREPSWEACAFHSVSKQLNPAFSTRYMDTVQGM 300
                         YYEVPIA HLEQ N EPSW+  AFH  S Q +P F + YMDT+QGM
Sbjct: 241 NIASRISCQNRKQPYYEVPIAHHLEQSNYEPSWKTSAFHRGSNQFSPVFPSHYMDTIQGM 300

Query: 301 EKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHVDRSRIVCSNSYVRHNNFAPATERI 360
           EK SFP+KILAR+ RLN VD+MRFMSIGADKSANHV++SRI+ SNS VR NN    TE I
Sbjct: 301 EKFSFPRKILARSPRLNTVDSMRFMSIGADKSANHVNQSRIIRSNSCVRWNNL---TECI 360

Query: 361 FRNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIE 420
           +R+   SSA+Y +SA+LKL PERFNSVDEVRG+IFLMAKDQHGCRFLQRKF+EGTDEDIE
Sbjct: 361 YRDH--SSAMYSKSASLKLFPERFNSVDEVRGRIFLMAKDQHGCRFLQRKFVEGTDEDIE 420

Query: 421 KIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTR 475
           KIF+EIIDHVVDLMVD FGNYLVQKLLEVCNE+QRM+ L RITQNHGE++++SCDMHGTR
Sbjct: 421 KIFDEIIDHVVDLMVDAFGNYLVQKLLEVCNENQRMQILHRITQNHGELVIISCDMHGTR 480

BLAST of Carg28123 vs. ExPASy TrEMBL
Match: A0A6J1D1G6 (pumilio homolog 12-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016483 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.7e-181
Identity = 349/642 (54.36%), Postives = 412/642 (64.17%), Query Frame = 0

Query: 1   MEDMGSELEIEDLARLIGDEIPNVASGNAHAEEKGLFSIPVGPYEETSIRNFHGNGNLMD 60
           ME+MG+ELE +DLARLIGDEIPNV SGNAH+EEK L S+ V  Y   S    H NGNLMD
Sbjct: 1   MEEMGTELEFDDLARLIGDEIPNVGSGNAHSEEKSLSSMSVNSYGGPSFGKLHRNGNLMD 60

Query: 61  GNISMSTLQQSSMDQMLTRKDTMPGDQSLALAF--------------------------- 120
           GNI MS LQQS MDQ+LTR++TMPG+QSL  AF                           
Sbjct: 61  GNIPMSRLQQSPMDQILTRQNTMPGNQSLTFAFEKLSLGDEAVAAVKGHAWKSVPGHAVL 120

Query: 121 -------------------------------SSIH------------------------- 180
                                          S +H                         
Sbjct: 121 LDDHYSDNLNKQFLNMDASMLAPWSSSNHLNSGVHDPYMTRLGIQNSPSRHVPEQYKKWQ 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FPVTSHRQQIYHNGRSPLRYIHPQQVNQRQIGWEEEQFYRIQEQHPYLQHLHNQQLECLL 240

Query: 241 -------------------KYYEVPIARHLEQLNREPSWEACAFHSVSKQLNPAFSTRYM 300
                              +Y+EVPI+ HLEQ + EPSW+ CAFH    QL+P FST YM
Sbjct: 241 PIQPHENITSRTSCQNRKQQYFEVPISHHLEQSDHEPSWKTCAFHRGPNQLSPVFSTHYM 300

Query: 301 DTVQGMEKVSFPKKILARNTRLNAVDAMRFMSIGADKSANHVDRSRIVCSNSYVRHNNFA 360
           DT+QGMEK+SFP+KILARN  LN VDA+RF+SIGADKS  HV++SRIV SNS +RHNN A
Sbjct: 301 DTIQGMEKISFPRKILARNHGLNTVDAVRFVSIGADKSPAHVNQSRIVRSNSCIRHNNLA 360

Query: 361 PATERIFRNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEG 420
           PATE I  + L SSALY +SAN KL+P++ NSV+EVRGKI+LMAKDQ+GCRFLQRKF+EG
Sbjct: 361 PATECICHDHLGSSALYSDSANFKLLPDKLNSVNEVRGKIYLMAKDQYGCRFLQRKFVEG 420

Query: 421 TDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSC 480
           T EDIEKIFNEIIDHVV+LM D FGNYLVQKLLEVCNEDQRM+ L +IT+NHGE++++SC
Sbjct: 421 TQEDIEKIFNEIIDHVVELMTDAFGNYLVQKLLEVCNEDQRMQILHKITRNHGELVIISC 480

BLAST of Carg28123 vs. TAIR 10
Match: AT5G56510.1 (pumilio 12 )

HSP 1 Score: 328.2 bits (840), Expect = 1.1e-89
Identity = 160/273 (58.61%), Postives = 211/273 (77.29%), Query Frame = 0

Query: 206 RNELSSSALYLESANLKLVPERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEK 265
           +N+L+   L +   NL L P+++NS+ E RGKI+ +AKDQHGCRFLQR F E    DIE 
Sbjct: 251 QNQLNED-LTMSLNNLSLQPQKYNSIAEARGKIYYLAKDQHGCRFLQRIFSEKDGNDIEM 310

Query: 266 IFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRA 325
           IFNEIID++ +LM+DPFGNYLVQKLLEVCNEDQRM+ +  IT+  G +I +SCDMHGTRA
Sbjct: 311 IFNEIIDYISELMMDPFGNYLVQKLLEVCNEDQRMQIVHSITRKPGLLIKISCDMHGTRA 370

Query: 326 VQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQHCLDYLMP-SSEVLFDAVRNS 385
           VQK++ET K +E++ +I+S+LK G+V L+KNVNGNHV Q CL YL+P   + LF+A    
Sbjct: 371 VQKIVETAKREEEISIIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAAITH 430

Query: 386 CVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRW 445
           CV+LATDRHGCCVLQK L  S    + +L+SEIA NAL++SQD +GNYV+Q++  L+ +W
Sbjct: 431 CVELATDRHGCCVLQKCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFELQLQW 490

Query: 446 YTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESA 478
            T  I++QLEG++ +L+MQK SSNVVEKCL+ A
Sbjct: 491 ATFEILEQLEGNYTELSMQKCSSNVVEKCLKLA 522

BLAST of Carg28123 vs. TAIR 10
Match: AT1G22240.1 (pumilio 8 )

HSP 1 Score: 255.4 bits (651), Expect = 9.3e-68
Identity = 119/255 (46.67%), Postives = 183/255 (71.76%), Query Frame = 0

Query: 226 ERFNSVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNY 285
           E    V E +G ++ MAKDQHGCRFLQ  F +G+  D   IF+E+I HVV+LM+DPFGNY
Sbjct: 191 ESLPKVSEFQGYVYFMAKDQHGCRFLQWIFEDGSALDALVIFSEVIPHVVELMMDPFGNY 250

Query: 286 LVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSS 345
           L+QKLL+VCNE+QR + +  +T   G++I +S + +GTR VQ+++E++KT++Q+ ++ S+
Sbjct: 251 LMQKLLDVCNEEQRTQIILMVTSEPGQLIRISLNAYGTRVVQRLVESIKTRKQISLVKSA 310

Query: 346 LKSGMVTLMKNVNGNHVAQHCLDYL-MPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTC 405
           L+ G + L++++NGNHV Q CL  L    +E +F+     C+D+AT RHGCCVLQK +  
Sbjct: 311 LRPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDATKFCIDIATHRHGCCVLQKCIAY 370

Query: 406 SNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQK 465
           S+ + R+ L++EI+ N+L ++QD YGNY VQF+L L+      A++ QL+G + +L+MQK
Sbjct: 371 SSGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLELRDFSAIAAMLAQLKGHYVELSMQK 430

Query: 466 YSSNVVEKCLESARE 480
           +SS++VE+CL    E
Sbjct: 431 FSSHMVERCLTHCPE 445

BLAST of Carg28123 vs. TAIR 10
Match: AT1G78160.1 (pumilio 7 )

HSP 1 Score: 249.2 bits (635), Expect = 6.7e-66
Identity = 123/290 (42.41%), Postives = 194/290 (66.90%), Query Frame = 0

Query: 187 CSNSYVRHNNFAPATERIFRNELSSSALYLESAN-LKLVPERFNSVDEVRGKIFLMAKDQ 246
           CS  +    +F    + I     ++ AL   + N  ++      ++ +++G ++LMAKDQ
Sbjct: 286 CSEPFSSDESFFMNGKSIDHQRSNTRALMSNNGNPTEICHPSLPNMCDIQGYVYLMAKDQ 345

Query: 247 HGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRR 306
           HGCRFLQR F EGT  D   IFNE+I HVV+LM+DPFGNYL+QKLL+VC E+QR + +  
Sbjct: 346 HGCRFLQRIFDEGTSVDAMIIFNEVIAHVVELMMDPFGNYLMQKLLDVCTEEQRTQIVLV 405

Query: 307 ITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQH 366
            T+  G++I +S + +GTR VQ+++ET+++ +Q+ ++  +L+ G + L+K++NGNHV Q 
Sbjct: 406 ATEEPGQLIRISLNAYGTRVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQR 465

Query: 367 CLDYL-MPSSEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVI 426
           CL  L    ++ +FDA    C ++AT RHGCCVLQK +  S    R+ LI+EI+ N+L++
Sbjct: 466 CLQCLSTEDNKFIFDAATKFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLL 525

Query: 427 SQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCL 475
           +QD +GNY VQF++ L+       ++ QL+G +  L+MQK+SS++VE+CL
Sbjct: 526 AQDPFGNYAVQFVIELRIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCL 575

BLAST of Carg28123 vs. TAIR 10
Match: AT4G08840.1 (pumilio 11 )

HSP 1 Score: 223.8 bits (569), Expect = 3.0e-58
Identity = 107/240 (44.58%), Postives = 168/240 (70.00%), Query Frame = 0

Query: 236 GKIFLMAKDQHGCRFLQRKFMEGTDEDIEKIFNEIIDHVVDLMVDPFGNYLVQKLLEVCN 295
           G ++LMAKDQ GCR LQ+   EG   D+  IF E+I++V++L  DPFGNYL+QKL+EVCN
Sbjct: 244 GSVYLMAKDQLGCRLLQKFVDEGNFVDVMIIFKEVINNVIELGTDPFGNYLIQKLIEVCN 303

Query: 296 EDQRMKFLRRITQNHGEIILVSCDMHGTRAVQKVIETLKTQEQVFMIVSSLKSGMVTLMK 355
           E+QR + L R+T   G ++ +S + +GTR VQK+IET+ T+EQ+ ++ S+L  G ++L +
Sbjct: 304 EEQRTQILIRLTSKPGLLVKISINNYGTRVVQKLIETVTTKEQISLVKSALVPGFLSLFR 363

Query: 356 NVNGNHVAQHCLDYLMPS-SEVLFDAVRNSCVDLATDRHGCCVLQKYLTCSNSVLRDNLI 415
            +NGNHV  +CL +  P+ ++ + +A    C+++AT RHGCCVLQ+ ++ S     + L+
Sbjct: 364 ELNGNHVILNCLKFFSPNDNKFILEAATKFCIEIATTRHGCCVLQRCVSYSVGEQHEKLV 423

Query: 416 SEIAHNALVISQDQYGNYVVQFILRLKRRWYTEAIVKQLEGSFGDLAMQKYSSNVVEKCL 475
            EI+ N+L+++QD +GNY+VQ+I+  K       ++ +L G++  LA QK+ S+VVEKCL
Sbjct: 424 DEISRNSLLLAQDPFGNYLVQYIIEKKVGGVN--VLFELRGNYVKLATQKFGSHVVEKCL 481

BLAST of Carg28123 vs. TAIR 10
Match: AT1G35730.1 (pumilio 9 )

HSP 1 Score: 219.9 bits (559), Expect = 4.4e-57
Identity = 117/275 (42.55%), Postives = 179/275 (65.09%), Query Frame = 0

Query: 209 LSSSALYLESANLKLVPERFN---SVDEVRGKIFLMAKDQHGCRFLQRKFMEGTDEDIEK 268
           L  + L  + +  K+ P+  N   S+ E+ G + LMAKDQ GCR LQ+   EGT  + + 
Sbjct: 222 LEDTVLIGQGSYGKMSPKSNNDLVSMVEIYGSVNLMAKDQIGCRVLQKLVEEGTFHEAKV 281

Query: 269 IFNEIIDHVVDLMVDPFGNYLVQKLLEVCNEDQRMKFLRRITQNHGEIILVSCDMHGTRA 328
           I   IIDHVV+L +DPFGNY+VQKL +V +E+QR   +  +T N  E+I +  + +GTR 
Sbjct: 282 ILLAIIDHVVELSMDPFGNYIVQKLFDVSDEEQRTLIVSVLTSNPRELIRICLNTYGTRV 341

Query: 329 VQKVIETLKTQEQVFMIVSSLKSGMVTLMKNVNGNHVAQHCLDYLMPS-SEVLFDAVRNS 388
           VQK+IET+KT++Q+ ++ S LK G + L+K++NGNHV Q CL  L P+ +E + +A    
Sbjct: 342 VQKMIETVKTKQQIALVKSGLKPGFLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAATKY 401

Query: 389 CVDLATDRHGCCVLQKYLTCSNSVLRDNLISEIAHNALVISQDQYGNYVVQFILRLKRRW 448
           C ++A  RHGCCVLQ  ++ S  + R+ L++EI+ N+L +SQD +GNYVVQ++  + ++ 
Sbjct: 402 CAEIAIHRHGCCVLQCCISNSVGLQRERLVAEISRNSLHLSQDPFGNYVVQYL--IDQQV 461

Query: 449 YTEAIVKQLEGSFGDLAMQKYSSNVVEKCLESARE 480
               ++ Q    + +LA QK+SS+V+EKCL    E
Sbjct: 462 SAVKLLVQFRMHYAELATQKFSSHVIEKCLRKYPE 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022014.18.8e-278100.00Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022925804.19.2e-25171.30pumilio homolog 12-like [Cucurbita moschata] >XP_022925813.1 pumilio homolog 12-... [more]
XP_023529396.13.9e-24971.45pumilio homolog 12-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529397.1 ... [more]
XP_022966848.11.1e-24369.81pumilio homolog 12-like [Cucurbita maxima] >XP_022966857.1 pumilio homolog 12-li... [more]
KAG7022006.14.6e-24269.96Pumilio-like 12 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9LVC31.6e-8858.61Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2[more]
Q9LM201.3e-6646.67Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU... [more]
Q9C9R69.4e-6542.41Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU... [more]
Q9LDW34.2e-5744.58Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2[more]
Q1PFN96.1e-5642.55Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EGB24.5e-25171.30pumilio homolog 12-like OS=Cucurbita moschata OX=3662 GN=LOC111433100 PE=4 SV=1[more]
A0A6J1HP575.3e-24469.81pumilio homolog 12-like OS=Cucurbita maxima OX=3661 GN=LOC111466429 PE=4 SV=1[more]
A0A6J1JJB13.0e-18357.53pumilio homolog 12-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484953 P... [more]
A0A6J1H9W23.0e-18357.21pumilio homolog 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461817... [more]
A0A6J1D1G63.7e-18154.36pumilio homolog 12-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101648... [more]
Match NameE-valueIdentityDescription
AT5G56510.11.1e-8958.61pumilio 12 [more]
AT1G22240.19.3e-6846.67pumilio 8 [more]
AT1G78160.16.7e-6642.41pumilio 7 [more]
AT4G08840.13.0e-5844.58pumilio 11 [more]
AT1G35730.14.4e-5742.55pumilio 9 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001313Pumilio RNA-binding repeatSMARTSM00025pum_5coord: 345..379
e-value: 0.12
score: 21.5
coord: 233..268
e-value: 8.4E-5
score: 32.0
coord: 452..482
e-value: 460.0
score: 0.1
coord: 269..304
e-value: 2.0E-9
score: 47.3
coord: 416..451
e-value: 0.065
score: 22.4
coord: 308..344
e-value: 0.0059
score: 25.8
coord: 380..415
e-value: 1.4E-4
score: 31.2
IPR001313Pumilio RNA-binding repeatPFAMPF00806PUFcoord: 315..344
e-value: 5.6E-6
score: 25.9
coord: 459..477
e-value: 8.8E-4
score: 18.9
coord: 385..417
e-value: 6.4E-10
score: 38.5
coord: 238..271
e-value: 1.7E-11
score: 43.5
coord: 350..369
e-value: 0.021
score: 14.5
coord: 422..438
e-value: 0.013
score: 15.2
coord: 273..305
e-value: 1.5E-9
score: 37.3
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 269..304
score: 12.373602
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 233..268
score: 8.780567
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 416..451
score: 9.130537
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 305..344
score: 8.500589
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 380..415
score: 9.083875
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 218..482
e-value: 4.0E-91
score: 307.5
NoneNo IPR availablePANTHERPTHR12537:SF147PUMILIO HOMOLOG 12coord: 97..479
NoneNo IPR availablePANTHERPTHR12537RNA BINDING PROTEIN PUMILIO-RELATEDcoord: 97..479
IPR033133Pumilio homology domainPROSITEPS50303PUM_HDcoord: 210..484
score: 71.175705
IPR033712Pumilio, RNA binding domainCDDcd07920Pumiliocoord: 236..484
e-value: 2.95662E-96
score: 291.803
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 230..477

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28123-RACarg28123-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding