Carg28122 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28122
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pumilio homolog 12-like
Location	Carg_Chr11: 3611243 .. 3614310 (+)
RNA-Seq Expression	Carg28122
Synteny	Carg28122

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCGACTCATGAAATGCCTGCGATGCCATTTCCTATATCGTCTCATCTCGAGAAAATGTTGCCGAGTGGCCTATCCCCTATGCATTTTATGCATCCACAACAAATGAATCATGGCCAAATGGGTTCGAATTGTACAAAAGAAGAGCTTGTCAACTCCTGCATGATGCAAGGGCAGCACCAGTACCTTCATGATCTTCATAATCAGCAACTGGAGTATTCAAATCAATTCGAGTCGTTTGGTAATGCTACATTCGGGAAGTTGCGGTTTTTGAGAAGTCCTAAGAAGAAATGTTGTTTTGAGTATCCAGTTTCCCACAATCTTGAGCAGTCTAAACATGATGGATTCTCCAATGGGGCTGCAAATTACGCGGGTTCAGGTATATCAAATCATGTTTTTTCGATACCGTATGTAGATACATTAGACGGCCAGGATAAAAGTTTCAAGCAATGTTCTCCTAGAAAGATCCCCATTATGACCCATGGACTGAATGTAGTTGATTGTATGAAAGTTCATCTGTCGATGCCGAGAAACACAAAATATTTTATGCGCGCCAAAACAGATATCTTTGTCAAAGTTGTTATTTTAGGCAGTTCGGTTTTTCCTTCAACTGCTACGGATTGCGTCTGTCATGAGAACTTAAGGGTATCTGCTATGTTGTCTAGTTATGCCAAACATAAGACGAGATACCGCCTCTACAATGTAATCTCTCGATGAAGCCAACGGGTAAAATATATCTCATGGCTAAAGACCAACACGGTTGTCGCTTCTTGCAACGGATGTTCAGTGAGGGTACTAAGGAAGATATGGACATGATTTTCAATGAAATCATTCAACATGTTTCTGAGCTCATGATAGATCCTTTTGGAAACTATTTAATACAGAAGCTCCTTGGAGTTTTGTGATGAAGATCAACGGCTTCAAATTCTTTACCAGGTTAACCGACCCGGTGAACTCATTCGAATTCGTGTAATATGCACGGGTATGCTCTATTCACTGTTTTGTCTCTTTACCTCCGTTCAGCCGATTTCATGTCTAAAATCTGTTTCGAAACTACAGGACTCGTGCTGTTCAGAAGCTTATTGAGACCCTCAAAACCCCGGAGCAGTTTTCCATGATTGTCTCCTTATTGAAGCCTGGAATAGTGATTCTCATGAAAAACATTAATGGTAACCATGTAGCACAGCATTGCTTGCAGTTTTTGGTGTCTGAATATATTGAGGTAAGGGGTCTCCTTTGGGGATTCGTTTTCGCAAACATTTTTGTGACGAAATGAAATGCTGGCAATGAATTTCTTTTGCAGTTCCTTTTCGATGCTGCAATGAAATGTTGTGCCGAGGTTGCCACTGATCGACATGGTTGTTGTGTGCTTCAAAAATGCCTTTCTTGTTCTGATCATAGACAACGAGATCGATTGCTAACCGCGGTTGTATGTAATGCTTTATTTCTTTCCCAAGACCAATACGGGTAATTCTACTTTTGATCTTTATATTGTTCGTGGACTCCGTTCTCTGTTGTTTTGCAGCATTGTGATTCGGTTCCTTAACTAGTTTTTCAACTCGTTGACTATGCATGATTTAGTCTCTAAACTTTAATATGCAACGATTGATCTTTAGAACTAGCACTTGATTTTGTGATAGTAATGTTTTGAAACTGAGCGTTTGGTTTTGCATTGGAACGATGCAGGAACAATGTTTTGCAATATGAGTCTACTTTTGATCTTTATAATGTTCATGGACTCCGTTCTCTGTAGTTTTGCAGCGTTGTGATTCGGTTCTTTAACTAGTTTTTCAACTCGTTGACTATGCATGATTTAGTCTCTAAACTTTTAATATGTAACGATCTTTGGAACTAGTGCTTTATTTTGTGATAGTGTTTCGAAACCGAGCGCTTGATTTTGCATTGGAACGATGCAGGAACTATGTTGTGCAATATGGCGTCTACTTTTGATCTTTATAATGTTCATGGACTCCGTTCTCTGTAGTTTTGCAGCATTGTGATTCGGTTCTTTAACTAGTTTTTCAACTCGTTGACTATGCATAATTTAGTCTCTGAACTTTTAATATACAATGACTGATCTTTGGAACTAGCGCTTGATTTTGTGATAGTAATGTTTCAAAACCGAGTGCTTGATTTTGCATTGGAACGATGTAGGAACTATGTTGTGCAATTTGCGTTCGACCTTGCGCGTAATCCTACTGCAATTCCTTGGGTGATATCTGGTATCTTGAAACGTTTAGAGGGCCATTTTGCGGACTTGTCGATTCAGAAATATAGCAGTAATGTTGTCGAAAAATGTGCTTCTATTGGAGAGGAATACCTGACGAAAATTATCGATGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTTAACCCATATGGAAACTACGCAGTTCAAGCAGTGTTGGCTCATTCTGGGATTTGCAAAGTGAGTATCAAACTTGAACTGTTTGATAAACGGTGATGAATGATTTGCTGAATTACATGAATGTTTGTGTTTGTTAAACAGGGTTCTTCTCTTCATGCTAATCTTGTGGCTGCAATCAGACCGCACGTCCCGTTGCTTAGGACGAACATGTACGGGAAGAAAGTGCTCGGAATGCTCGGAAAAGTGAACTGACCGACGCAATCGACTAAGATTGGCTGTAACCTGCATTTTTTGTTGTTGTTGTTGGTCAAAAAAGGACAGGACATGGCTTGTCTTTCTGCTCTCAAGTGTTGCTGCTGTTTTGTTCTATTGTTCTTTTGTTTGTCAGGAGCTTAGCTGCAAGTGTTCAACTAGTGTTTCTCCTCAAGTCACTTGTTATTTTGTTCTCTTGTTCTCTTGTTCTTTTGTTTGTTAAGGAGCTTAGGCCATTAACCATGGCTGCAAGTGTGCAACTAGGTCTTATTATTTTGTTCTTTTGTTTGTTTGTTAAGGAGCTTAAGCCATTTAACATGGTTCGTCTCCATCTCTATCCATGGTTCATCCCCGGACGATGAACGGATTTCCCTTTTTGTAGAGTCATACAAGAGTCATTGTTGTATTCGAGATCGAGATGACAATGAATGATAAGCTT

mRNA sequence

ATGCCATCGACTCATGAAATGCCTGCGATGCCATTTCCTATATCGTCTCATCTCGAGAAAATGTTGCCGAGTGGCCTATCCCCTATGCATTTTATGCATCCACAACAAATGAATCATGGCCAAATGGGTTCGAATTGTACAAAAGAAGAGCTTGTCAACTCCTGCATGATGCAAGGGCAGCACCAGTACCTTCATGATCTTCATAATCAGCAACTGGAGTATTCAAATCAATTCGAGTCGTTTGGTAATGCTACATTCGGGAAGTTGCGGTTTTTGAGAAGTCCTAAGAAGAAATGTTGTTTTGAGTATCCAGTTTCCCACAATCTTGAGCAGTCTAAACATGATGGATTCTCCAATGGGGCTGCAAATTACGCGGGTTCAGAAGCTCCTTGGAGTTTTGTGATGAAGATCAACGGCTTCAAATTCTTTACCAGGTTAACCGACCCGGTGAACTCATTCGAATTCGTGACTCGTGCTGTTCAGAAGCTTATTGAGACCCTCAAAACCCCGGAGCAGTTTTCCATGATTGTCTCCTTATTGAAGCCTGGAATAGTGATTCTCATGAAAAACATTAATGGTAACCATGTAGCACAGCATTGCTTGCAGTTTTTGGTGTCTGAATATATTGAGTTCCTTTTCGATGCTGCAATGAAATGTTGTGCCGAGGTTGCCACTGATCGACATGGTTGTTGTGTGCTTCAAAAATGCCTTTCTTGTTCTGATCATAGACAACGAGATCGATTGCTAACCGCGGTTGTATGTAATGCTTTATTTCTTTCCCAAGACCAATACGGGAACTATGTTGTGCAATTTGCGTTCGACCTTGCGCGTAATCCTACTGCAATTCCTTGGGTGATATCTGGTATCTTGAAACGTTTAGAGGGCCATTTTGCGGACTTGTCGATTCAGAAATATAGCAGTAATGTTGTCGAAAAATGTGCTTCTATTGGAGAGGAATACCTGACGAAAATTATCGATGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTTAACCCATATGGAAACTACGCAGTTCAAGCAGTGTTGGCTCATTCTGGGATTTGCAAAGGTTCTTCTCTTCATGCTAATCTTGTGGCTGCAATCAGACCGCACGTCCCGTTGCTTAGGACGAACATGTACGGGAAGAAAGTGCTCGGAATGCTCGGAAAAGTGAACTGACCGACGCAATCGACTAAGATTGGCTGTAACCTGCATTTTTTGTTGTTGTTGTTGGTCAAAAAAGGACAGGACATGGCTTGTCTTTCTGCTCTCAAGTGTTGCTGCTGTTTTGTTCTATTGTTCTTTTGTTTGTCAGGAGCTTAGCTGCAAGTGTTCAACTAGTGTTTCTCCTCAAGTCACTTGTTATTTTGTTCTCTTGTTCTCTTGTTCTTTTGTTTGTTAAGGAGCTTAGGCCATTAACCATGGCTGCAAGTGTGCAACTAGGTCTTATTATTTTGTTCTTTTGTTTGTTTGTTAAGGAGCTTAAGCCATTTAACATGGTTCGTCTCCATCTCTATCCATGGTTCATCCCCGGACGATGAACGGATTTCCCTTTTTGTAGAGTCATACAAGAGTCATTGTTGTATTCGAGATCGAGATGACAATGAATGATAAGCTT

Coding sequence (CDS)

ATGCCATCGACTCATGAAATGCCTGCGATGCCATTTCCTATATCGTCTCATCTCGAGAAAATGTTGCCGAGTGGCCTATCCCCTATGCATTTTATGCATCCACAACAAATGAATCATGGCCAAATGGGTTCGAATTGTACAAAAGAAGAGCTTGTCAACTCCTGCATGATGCAAGGGCAGCACCAGTACCTTCATGATCTTCATAATCAGCAACTGGAGTATTCAAATCAATTCGAGTCGTTTGGTAATGCTACATTCGGGAAGTTGCGGTTTTTGAGAAGTCCTAAGAAGAAATGTTGTTTTGAGTATCCAGTTTCCCACAATCTTGAGCAGTCTAAACATGATGGATTCTCCAATGGGGCTGCAAATTACGCGGGTTCAGAAGCTCCTTGGAGTTTTGTGATGAAGATCAACGGCTTCAAATTCTTTACCAGGTTAACCGACCCGGTGAACTCATTCGAATTCGTGACTCGTGCTGTTCAGAAGCTTATTGAGACCCTCAAAACCCCGGAGCAGTTTTCCATGATTGTCTCCTTATTGAAGCCTGGAATAGTGATTCTCATGAAAAACATTAATGGTAACCATGTAGCACAGCATTGCTTGCAGTTTTTGGTGTCTGAATATATTGAGTTCCTTTTCGATGCTGCAATGAAATGTTGTGCCGAGGTTGCCACTGATCGACATGGTTGTTGTGTGCTTCAAAAATGCCTTTCTTGTTCTGATCATAGACAACGAGATCGATTGCTAACCGCGGTTGTATGTAATGCTTTATTTCTTTCCCAAGACCAATACGGGAACTATGTTGTGCAATTTGCGTTCGACCTTGCGCGTAATCCTACTGCAATTCCTTGGGTGATATCTGGTATCTTGAAACGTTTAGAGGGCCATTTTGCGGACTTGTCGATTCAGAAATATAGCAGTAATGTTGTCGAAAAATGTGCTTCTATTGGAGAGGAATACCTGACGAAAATTATCGATGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTTAACCCATATGGAAACTACGCAGTTCAAGCAGTGTTGGCTCATTCTGGGATTTGCAAAGGTTCTTCTCTTCATGCTAATCTTGTGGCTGCAATCAGACCGCACGTCCCGTTGCTTAGGACGAACATGTACGGGAAGAAAGTGCTCGGAATGCTCGGAAAAGTGAACTGA

Protein sequence

MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQHQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNGAANYAGSEAPWSFVMKINGFKFFTRLTDPVNSFEFVTRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLARNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQIMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKVLGMLGKVN

Homology

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BLAST of Carg28122 vs. NCBI nr
Match: KAG7022013.1 (Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805.4 bits (2079), Expect = 2.1e-229
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ
Sbjct: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120

Query: 121 AANYAGSEAPWSFVMKINGFKFFTRLTDPVNSFEFVTRAVQKLIETLKTPEQFSMIVSLL 180
           AANYAGSEAPWSFVMKINGFKFFTRLTDPVNSFEFVTRAVQKLIETLKTPEQFSMIVSLL
Sbjct: 121 AANYAGSEAPWSFVMKINGFKFFTRLTDPVNSFEFVTRAVQKLIETLKTPEQFSMIVSLL 180

Query: 181 KPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCS 240
           KPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCS
Sbjct: 181 KPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCS 240

Query: 241 DHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLARNPTAIPWVISGILKRLEGHFADL 300
           DHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLARNPTAIPWVISGILKRLEGHFADL
Sbjct: 241 DHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLARNPTAIPWVISGILKRLEGHFADL 300

Query: 301 SIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQIMLNPYGNYAVQAVLAHSGICKGS 360
           SIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQIMLNPYGNYAVQAVLAHSGICKGS
Sbjct: 301 SIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQIMLNPYGNYAVQAVLAHSGICKGS 360

Query: 361 SLHANLVAAIRPHVPLLRTNMYGKKVLGMLGKVN 395
           SLHANLVAAIRPHVPLLRTNMYGKKVLGMLGKVN
Sbjct: 361 SLHANLVAAIRPHVPLLRTNMYGKKVLGMLGKVN 394

[Close]

BLAST of Carg28122 vs. NCBI nr
Match: XP_022930793.1 (pumilio homolog 12-like [Cucurbita moschata])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 376/566 (66.43%), Postives = 384/566 (67.84%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ
Sbjct: 167 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 226

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 227 HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 286

Query: 121 AANYAGS-------EAPW---------SF-------------------VMKI-------- 180
           AANYAGS         P+         SF                    MK+        
Sbjct: 287 AANYAGSGISNHVFSIPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 346

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 347 KYFMRQNRYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 406

Query: 241 ------------NGFKFFTR------------------------LTDPVNSF-------- 300
                       +G +F  R                        + DP  ++        
Sbjct: 407 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDMDMIFNEIIQHVSELMIDPFGNYLIQKLLGV 466

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 467 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 526

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 395
           KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 527 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 586

[Close]

BLAST of Carg28122 vs. NCBI nr
Match: KAG6588121.1 (Pumilio-like 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.9 bits (1740), Expect = 4.3e-190
Identity = 375/566 (66.25%), Postives = 383/566 (67.67%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ
Sbjct: 167 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 226

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 227 HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 286

Query: 121 AANYAGS-------EAPW---------SF-------------------VMKI-------- 180
           AANYAGS         P+         SF                    MK+        
Sbjct: 287 AANYAGSGISNHVFSTPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 346

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 347 KYFMRQNRYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 406

Query: 241 ------------NGFKFFTR------------------------LTDPVNSF-------- 300
                       +G +F  R                        + DP  ++        
Sbjct: 407 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDINMIFNEIIQHVSELMIDPFGNYLIQKLLGV 466

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 467 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 526

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 395
           KNINGNHVAQHCLQFLVSEYIEFLFDAA KCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 527 KNINGNHVAQHCLQFLVSEYIEFLFDAATKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 586

[Close]

BLAST of Carg28122 vs. NCBI nr
Match: XP_023530364.1 (uncharacterized protein LOC111792962 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-188
Identity = 374/566 (66.08%), Postives = 382/566 (67.49%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNS MMQGQ
Sbjct: 167 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSGMMQGQ 226

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 227 HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 286

Query: 121 AANYAGS-------EAPW---------SF-------------------VMKI-------- 180
           AANYAGS         P+         SF                    MK+        
Sbjct: 287 AANYAGSGISNHVFSTPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 346

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 347 KYFMRQNRYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 406

Query: 241 ------------NGFKFFTR------------------------LTDPVNSF-------- 300
                       +G +F  R                        + DP  ++        
Sbjct: 407 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDIDMIFNEIIQHVSELMIDPFGNYLIQKLLGV 466

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 467 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 526

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 395
           KNINGNHVAQHCLQFLVSEYIEFLFDAA KCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 527 KNINGNHVAQHCLQFLVSEYIEFLFDAATKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 586

[Close]

BLAST of Carg28122 vs. NCBI nr
Match: KAG7022005.1 (Pumilio-like 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-188
Identity = 372/564 (65.96%), Postives = 381/564 (67.55%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQG+
Sbjct: 167 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGK 226

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 227 HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 286

Query: 121 AANYAGS-------EAPW---------SF-------------------VMKI-------- 180
           AANYAGS         P+         SF                    MK+        
Sbjct: 287 AANYAGSGISNHVFSTPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 346

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 347 KYFMRQNRYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 406

Query: 241 ------------NGFKFFTR------------------------LTDPVNSF-------- 300
                       +G +F  R                        + DP  ++        
Sbjct: 407 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDINMIFNEIIQHVSELMIDPFGNYLIQKLLGV 466

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 467 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 526

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 393
           KNINGNHVAQHCLQFLVSEYIEFLFDAA KCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 527 KNINGNHVAQHCLQFLVSEYIEFLFDAATKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 586

[Close]

BLAST of Carg28122 vs. ExPASy Swiss-Prot
Match: Q9LVC3 (Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2)

HSP 1 Score: 236.5 bits (602), Expect = 5.1e-61
Identity = 125/235 (53.19%), Postives = 167/235 (71.06%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TRAVQK++ET K  E+ S+I+S LK GIV L+KN+NGNHV Q CLQ+L+    +FLF+AA
Sbjct: 367 TRAVQKIVETAKREEEISIIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAA 426

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
           +  C E+ATDRHGCCVLQKCL  S+  Q+  L++ +  NAL LSQD +GNYV+Q+ F+L 
Sbjct: 427 ITHCVELATDRHGCCVLQKCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFEL- 486

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEE-YLTKIIDELINDERFS 336
                + W    IL++LEG++ +LS+QK SSNVVEKC  + ++ +  +II ELIN  R  
Sbjct: 487 ----QLQWATFEILEQLEGNYTELSMQKCSSNVVEKCLKLADDKHRARIIRELINYGRLD 546

Query: 337 QIMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKVLGML 391
           Q+ML+PYGNY +QA L  S   KG ++HA LV AI+ ++  LRTN YGKKVL  L
Sbjct: 547 QVMLDPYGNYVIQAALKQS---KG-NVHALLVDAIKLNISSLRTNPYGKKVLSAL 592

[Close]

BLAST of Carg28122 vs. ExPASy Swiss-Prot
Match: Q9LM20 (Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM8 PE=3 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 2.6e-52
Identity = 109/230 (47.39%), Postives = 162/230 (70.43%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQ+L+E++KT +Q S++ S L+PG + L++++NGNHV Q CLQ L +E  EF+F+ A
Sbjct: 288 TRVVQRLVESIKTRKQISLVKSALRPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDA 347

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C ++AT RHGCCVLQKC++ S   QR++L+T +  N+LFL+QD YGNY VQF  +L 
Sbjct: 348 TKFCIDIATHRHGCCVLQKCIAYSSGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLEL- 407

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
           R+ +A    I+ +L +L+GH+ +LS+QK+SS++VE+C +   E   +I+ ELI+   F  
Sbjct: 408 RDFSA----IAAMLAQLKGHYVELSMQKFSSHMVERCLTHCPESRPQIVRELISVPHFDI 467

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY N+ +QA LA   + KG SLHA LV  IRPH  +LR N Y K++
Sbjct: 468 LIQDPYANFVIQAALA---VTKG-SLHATLVEVIRPH-SILRNNPYCKRI 507

[Close]

BLAST of Carg28122 vs. ExPASy Swiss-Prot
Match: Q9C9R6 (Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM7 PE=3 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-51
Identity = 105/230 (45.65%), Postives = 158/230 (68.70%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQ+L+ET+++ +Q S++   L+PG + L+K++NGNHV Q CLQ L +E  +F+FDAA
Sbjct: 423 TRVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQRCLQCLSTEDNKFIFDAA 482

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C E+AT RHGCCVLQKC++ S  +QR++L+  +  N+L L+QD +GNY VQF  +L 
Sbjct: 483 TKFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLLAQDPFGNYAVQFVIEL- 542

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
                IP  ++ +L +L+GH+  LS+QK+SS++VE+C     E   +I+ EL++   F Q
Sbjct: 543 ----RIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCLMHCPESRPQIVRELVSVPHFDQ 602

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY N+ +QA LA +   KG  LHA+LV  IRPH  +LR N Y K++
Sbjct: 603 LLQDPYANFVIQAALAAT---KG-PLHASLVEVIRPH-SILRNNPYCKRI 642

[Close]

BLAST of Carg28122 vs. ExPASy Swiss-Prot
Match: Q1PFN9 (Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.4e-42
Identity = 99/230 (43.04%), Postives = 145/230 (63.04%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQK+IET+KT +Q +++ S LKPG + L+K++NGNHV Q CLQ L     EF+ +AA
Sbjct: 339 TRVVQKMIETVKTKQQIALVKSGLKPGFLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAA 398

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K CAE+A  RHGCCVLQ C+S S   QR+RL+  +  N+L LSQD +GNYVVQ+  D  
Sbjct: 399 TKYCAEIAIHRHGCCVLQCCISNSVGLQRERLVAEISRNSLHLSQDPFGNYVVQYLID-- 458

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
           +  +A+      +L +   H+A+L+ QK+SS+V+EKC     E   +I+ EL+    F  
Sbjct: 459 QQVSAVK-----LLVQFRMHYAELATQKFSSHVIEKCLRKYPESRAEIVRELLCVPNFEY 518

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY NY +Q  L+   + KG  + A LVA +  +   L ++ Y KK+
Sbjct: 519 LLQDPYANYVIQTALS---VTKG-PVRAKLVAKVYRY-GKLHSSPYCKKI 556

[Close]

BLAST of Carg28122 vs. ExPASy Swiss-Prot
Match: Q9LDW3 (Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 7.2e-39
Identity = 88/223 (39.46%), Postives = 134/223 (60.09%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQKLIET+ T EQ S++ S L PG + L + +NGNHV  +CL+F      +F+ +AA
Sbjct: 331 TRVVQKLIETVTTKEQISLVKSALVPGFLSLFRELNGNHVILNCLKFFSPNDNKFILEAA 390

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C E+AT RHGCCVLQ+C+S S   Q ++L+  +  N+L L+QD +GNY+VQ+  +  
Sbjct: 391 TKFCIEIATTRHGCCVLQRCVSYSVGEQHEKLVDEISRNSLLLAQDPFGNYLVQYIIEKK 450

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
                       +L  L G++  L+ QK+ S+VVEKC     E  ++I++EL++   F  
Sbjct: 451 VGGV-------NVLFELRGNYVKLATQKFGSHVVEKCLRYYPESRSQIVNELVSVLNFGY 510

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRT 380
           ++ +PY NY +Q  L+ +   KG  + A+LV  +R +  L  T
Sbjct: 511 LLQDPYANYVIQCALSKT---KG-FVRASLVEKVRRYENLKMT 542

[Close]

BLAST of Carg28122 vs. ExPASy TrEMBL
Match: A0A6J1EWD9 (pumilio homolog 12-like OS=Cucurbita moschata OX=3662 GN=LOC111437164 PE=4 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.5e-191
Identity = 376/566 (66.43%), Postives = 384/566 (67.84%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ
Sbjct: 167 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 226

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 227 HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 286

Query: 121 AANYAGS-------EAPW---------SF-------------------VMKI-------- 180
           AANYAGS         P+         SF                    MK+        
Sbjct: 287 AANYAGSGISNHVFSIPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 346

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 347 KYFMRQNRYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 406

Query: 241 ------------NGFKFFTR------------------------LTDPVNSF-------- 300
                       +G +F  R                        + DP  ++        
Sbjct: 407 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDMDMIFNEIIQHVSELMIDPFGNYLIQKLLGV 466

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 467 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 526

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 395
           KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 527 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 586

[Close]

BLAST of Carg28122 vs. ExPASy TrEMBL
Match: A0A6J1HUY8 (pumilio homolog 12-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466416 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 369/566 (65.19%), Postives = 378/566 (66.78%), Query Frame = 0


Query: 1   MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQGQ 60
           MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMG NCTKEELVNSCMMQGQ
Sbjct: 175 MPSTHEMPAMPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGLNCTKEELVNSCMMQGQ 234

Query: 61  HQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 120
           HQYLHDLHNQQ EYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG
Sbjct: 235 HQYLHDLHNQQQEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFSNG 294

Query: 121 AANYAGS-------EAPW------------------------------------------ 180
            ANY GS         P+                                          
Sbjct: 295 TANYVGSGISNHVFSTPYVDTLDGQDKSFKQCSPRKIPIMTHGLNVVDCMKVPSVDAEKQ 354

Query: 181 SFVMKIN----------------------------------------------------- 240
            + M++N                                                     
Sbjct: 355 KYFMRLNSYLCQSCYFRQFGFPSTATDCVCHENLRVSAMLSSYAKHKTEIPPLQCNSLDE 414

Query: 241 -------------GFKFFTR------------------------LTDPVNSF-------- 300
                        G +F  R                        + DP  ++        
Sbjct: 415 ATGKIYLMAKDQHGCRFLQRMFSEGTKEDIDMIFNEIIQHVSELMIDPFGNYLIQKLLGV 474

Query: 301 -----------------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 360
                            E +        TRAVQKLIETLKTPEQFSMIVSLLKPGIVILM
Sbjct: 475 CDEDQRLQILYQVNRPGELIRISCNMHGTRAVQKLIETLKTPEQFSMIVSLLKPGIVILM 534

Query: 361 KNINGNHVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 395
           KNINGNHVAQHCLQFLVSEYIEFLFDAA KCCAEVATDRHGCCVLQKCLSCSDHRQRDRL
Sbjct: 535 KNINGNHVAQHCLQFLVSEYIEFLFDAATKCCAEVATDRHGCCVLQKCLSCSDHRQRDRL 594

[Close]

BLAST of Carg28122 vs. ExPASy TrEMBL
Match: A0A0A0LZQ0 (PUM-HD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G599460 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.5e-129
Identity = 282/560 (50.36%), Postives = 321/560 (57.32%), Query Frame = 0


Query: 1   MPSTHEMPA--MPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQ 60
           MP THE+PA  + F + SH ++ML +GLSP+HFMHPQQMNHG++G N  K E ++SC MQ
Sbjct: 188 MPFTHEVPARNLQFSLPSHHDQMLLNGLSPVHFMHPQQMNHGEIGPNSVKGEQLHSCRMQ 247

Query: 61  GQHQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFS 120
            Q QYLHDLHNQQLE SN FES GNA FG LRF +SPK++   E P     EQSKH+GF 
Sbjct: 248 WQQQYLHDLHNQQLERSNLFESCGNAAFGSLRF-QSPKQQRFVEVPFHPCREQSKHEGFC 307

Query: 121 NGAANYAGSEAP-----------------------------------------WSFVMKI 180
           NGAA+ A S  P                                           +    
Sbjct: 308 NGAAHCAASGIPNHAFATPFLDTLDAQEKCFKQSSPRKIPTRAHGLIGVDTEKLKYYFSQ 367

Query: 181 NGF--------------------------------------------------------- 240
           NGF                                                         
Sbjct: 368 NGFLCPSCYVRQYGFPSTAKDCICHDNFRVSSMLSSHANRKTEIPPLKCNSLDEATGKIY 427

Query: 241 ---------KFFTR------------------------LTDPVNSF-------------- 300
                    +F  R                        + DP  ++              
Sbjct: 428 LMAKDQHGCRFLQRMFSEGTKEDIEMIFGEIIHHVSELMVDPFGNYLIQKLLGVCDEDQR 487

Query: 301 -----------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGN 360
                      E +        TRAVQKLIETLKTPEQFS+IVSLLK GIVILMKN+NGN
Sbjct: 488 LQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSLLKTGIVILMKNVNGN 547

Query: 361 HVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVC 395
           HVAQHCLQ+L+ +YI FLFDAA K C EVATDRHGCCVLQKCL+ SD R RDRLL+ VV 
Sbjct: 548 HVAQHCLQYLMPDYIGFLFDAATKSCVEVATDRHGCCVLQKCLAVSDARHRDRLLSEVVR 607

[Close]

BLAST of Carg28122 vs. ExPASy TrEMBL
Match: A0A5A7UN64 (Pumilio-like protein 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001060 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 3.0e-128
Identity = 282/560 (50.36%), Postives = 323/560 (57.68%), Query Frame = 0


Query: 1   MPSTHEMPA--MPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQ 60
           MP T+E+PA  + FP+SSH ++ML +GLSP+HFMH QQMNHG++G NC K E ++SC MQ
Sbjct: 188 MPFTNEVPARNLQFPLSSHHDQMLLNGLSPVHFMHSQQMNHGEIGPNCAKGEQLHSCRMQ 247

Query: 61  GQHQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFS 120
            Q QYLHDLHNQQLE SN FES GNA FG LRF +SPK++   E P     EQSKH+GF 
Sbjct: 248 WQQQYLHDLHNQQLERSNLFESCGNAAFG-LRF-QSPKQQRFVEVPFHPCREQSKHEGFC 307

Query: 121 NGAANYAGSEAP-----------------------------------------WSFVMKI 180
           NGAA+ A S  P                                           +    
Sbjct: 308 NGAAHCAASGIPNHAFASPYLDTLDAQEKCFKQSSPRKVPTRAHGLIGVDTEKLKYYFSQ 367

Query: 181 NGF--------------------------------------------------------- 240
           NGF                                                         
Sbjct: 368 NGFLCPSCYVRQYGFPSTAKDCICHDNFRVPSMLSSNANRNTEIPPLKCNSLDEASGKIY 427

Query: 241 ---------KFFTR------------------------LTDPVNSF-------------- 300
                    +F  R                        + DP  ++              
Sbjct: 428 LMAKDQHGCRFLQRMFSEGTKEDIEIIFGEIIHHVSELMVDPFGNYLIQKLLGVCDEDQR 487

Query: 301 -----------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGN 360
                      E +        TRAVQKLIETLKTPEQFS+IVSLLK GIVILMKN+NGN
Sbjct: 488 LQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSLLKTGIVILMKNVNGN 547

Query: 361 HVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVC 395
           HVAQHCLQ+L+ +YI FLF+AA K C EVATDRHGCCVLQKCL+ SD R RDRLL+ VV 
Sbjct: 548 HVAQHCLQYLMPDYIGFLFEAATKSCVEVATDRHGCCVLQKCLAVSDARHRDRLLSEVVR 607

[Close]

BLAST of Carg28122 vs. ExPASy TrEMBL
Match: A0A1S4DY70 (uncharacterized protein LOC103492417 OS=Cucumis melo OX=3656 GN=LOC103492417 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.5e-127
Identity = 281/560 (50.18%), Postives = 322/560 (57.50%), Query Frame = 0


Query: 1   MPSTHEMPA--MPFPISSHLEKMLPSGLSPMHFMHPQQMNHGQMGSNCTKEELVNSCMMQ 60
           MP T+E+PA  + FP+SSH ++ML +GLSP+HFMH QQMNHG++G NC K E ++SC MQ
Sbjct: 188 MPFTNEVPARNLQFPLSSHRDQMLLNGLSPVHFMHSQQMNHGEIGPNCAKGEQLHSCRMQ 247

Query: 61  GQHQYLHDLHNQQLEYSNQFESFGNATFGKLRFLRSPKKKCCFEYPVSHNLEQSKHDGFS 120
            Q QYLHDLHNQQLE SN FES GNA FG LRF +SPK++   E P     EQSKH+GF 
Sbjct: 248 WQQQYLHDLHNQQLERSNLFESCGNAAFG-LRF-QSPKQQRFVEVPFHPCREQSKHEGFC 307

Query: 121 NGAANYAGSEAP-----------------------------------------WSFVMKI 180
           NGAA+ A S  P                                           +    
Sbjct: 308 NGAAHCAASGIPNHAFASPYLDTLDAQEKCFKQSSPRKVPTRAHGLIGVDTEKLKYYFSQ 367

Query: 181 NGF--------------------------------------------------------- 240
           NGF                                                         
Sbjct: 368 NGFLCPSCYVRQYGFPSTAKDCICHDNFRVPSMLSSNANRNTEIPPLKCNSLDEASGKIY 427

Query: 241 ---------KFFTR------------------------LTDPVNSF-------------- 300
                    +F  R                        + DP  ++              
Sbjct: 428 LMAKDQHGCRFLQRMFSEGTKEDIEIIFGEIIHHVSELMVDPFGNYLIQKLLGVCDEDQR 487

Query: 301 -----------EFV--------TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGN 360
                      E +        TRAVQKLIETLKTPEQFS+IVSLLK GIVILMKN+NGN
Sbjct: 488 LQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSLLKTGIVILMKNVNGN 547

Query: 361 HVAQHCLQFLVSEYIEFLFDAAMKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVC 395
           HVAQHCLQ+L+ +YI FLF+AA K C EVATDRHGCCVLQKCL+ SD   RDRLL+ VV 
Sbjct: 548 HVAQHCLQYLMPDYIGFLFEAATKSCVEVATDRHGCCVLQKCLAVSDAGHRDRLLSEVVR 607

[Close]

BLAST of Carg28122 vs. TAIR 10
Match: AT5G56510.1 (pumilio 12 )

HSP 1 Score: 236.5 bits (602), Expect = 3.7e-62
Identity = 125/235 (53.19%), Postives = 167/235 (71.06%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TRAVQK++ET K  E+ S+I+S LK GIV L+KN+NGNHV Q CLQ+L+    +FLF+AA
Sbjct: 367 TRAVQKIVETAKREEEISIIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAA 426

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
           +  C E+ATDRHGCCVLQKCL  S+  Q+  L++ +  NAL LSQD +GNYV+Q+ F+L 
Sbjct: 427 ITHCVELATDRHGCCVLQKCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFEL- 486

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEE-YLTKIIDELINDERFS 336
                + W    IL++LEG++ +LS+QK SSNVVEKC  + ++ +  +II ELIN  R  
Sbjct: 487 ----QLQWATFEILEQLEGNYTELSMQKCSSNVVEKCLKLADDKHRARIIRELINYGRLD 546

Query: 337 QIMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKVLGML 391
           Q+ML+PYGNY +QA L  S   KG ++HA LV AI+ ++  LRTN YGKKVL  L
Sbjct: 547 QVMLDPYGNYVIQAALKQS---KG-NVHALLVDAIKLNISSLRTNPYGKKVLSAL 592

[Close]

BLAST of Carg28122 vs. TAIR 10
Match: AT1G22240.1 (pumilio 8 )

HSP 1 Score: 207.6 bits (527), Expect = 1.8e-53
Identity = 109/230 (47.39%), Postives = 162/230 (70.43%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQ+L+E++KT +Q S++ S L+PG + L++++NGNHV Q CLQ L +E  EF+F+ A
Sbjct: 288 TRVVQRLVESIKTRKQISLVKSALRPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDA 347

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C ++AT RHGCCVLQKC++ S   QR++L+T +  N+LFL+QD YGNY VQF  +L 
Sbjct: 348 TKFCIDIATHRHGCCVLQKCIAYSSGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLEL- 407

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
           R+ +A    I+ +L +L+GH+ +LS+QK+SS++VE+C +   E   +I+ ELI+   F  
Sbjct: 408 RDFSA----IAAMLAQLKGHYVELSMQKFSSHMVERCLTHCPESRPQIVRELISVPHFDI 467

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY N+ +QA LA   + KG SLHA LV  IRPH  +LR N Y K++
Sbjct: 468 LIQDPYANFVIQAALA---VTKG-SLHATLVEVIRPH-SILRNNPYCKRI 507

[Close]

BLAST of Carg28122 vs. TAIR 10
Match: AT1G78160.1 (pumilio 7 )

HSP 1 Score: 205.3 bits (521), Expect = 9.0e-53
Identity = 105/230 (45.65%), Postives = 158/230 (68.70%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQ+L+ET+++ +Q S++   L+PG + L+K++NGNHV Q CLQ L +E  +F+FDAA
Sbjct: 423 TRVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQRCLQCLSTEDNKFIFDAA 482

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C E+AT RHGCCVLQKC++ S  +QR++L+  +  N+L L+QD +GNY VQF  +L 
Sbjct: 483 TKFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLLAQDPFGNYAVQFVIEL- 542

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
                IP  ++ +L +L+GH+  LS+QK+SS++VE+C     E   +I+ EL++   F Q
Sbjct: 543 ----RIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCLMHCPESRPQIVRELVSVPHFDQ 602

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY N+ +QA LA +   KG  LHA+LV  IRPH  +LR N Y K++
Sbjct: 603 LLQDPYANFVIQAALAAT---KG-PLHASLVEVIRPH-SILRNNPYCKRI 642

[Close]

BLAST of Carg28122 vs. TAIR 10
Match: AT1G35730.1 (pumilio 9 )

HSP 1 Score: 173.3 bits (438), Expect = 3.8e-43
Identity = 99/230 (43.04%), Postives = 145/230 (63.04%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQK+IET+KT +Q +++ S LKPG + L+K++NGNHV Q CLQ L     EF+ +AA
Sbjct: 339 TRVVQKMIETVKTKQQIALVKSGLKPGFLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAA 398

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K CAE+A  RHGCCVLQ C+S S   QR+RL+  +  N+L LSQD +GNYVVQ+  D  
Sbjct: 399 TKYCAEIAIHRHGCCVLQCCISNSVGLQRERLVAEISRNSLHLSQDPFGNYVVQYLID-- 458

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
           +  +A+      +L +   H+A+L+ QK+SS+V+EKC     E   +I+ EL+    F  
Sbjct: 459 QQVSAVK-----LLVQFRMHYAELATQKFSSHVIEKCLRKYPESRAEIVRELLCVPNFEY 518

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRTNMYGKKV 387
           ++ +PY NY +Q  L+   + KG  + A LVA +  +   L ++ Y KK+
Sbjct: 519 LLQDPYANYVIQTALS---VTKG-PVRAKLVAKVYRY-GKLHSSPYCKKI 556

[Close]

BLAST of Carg28122 vs. TAIR 10
Match: AT4G08840.1 (pumilio 11 )

HSP 1 Score: 162.9 bits (411), Expect = 5.1e-40
Identity = 88/223 (39.46%), Postives = 134/223 (60.09%), Query Frame = 0


Query: 157 TRAVQKLIETLKTPEQFSMIVSLLKPGIVILMKNINGNHVAQHCLQFLVSEYIEFLFDAA 216
           TR VQKLIET+ T EQ S++ S L PG + L + +NGNHV  +CL+F      +F+ +AA
Sbjct: 331 TRVVQKLIETVTTKEQISLVKSALVPGFLSLFRELNGNHVILNCLKFFSPNDNKFILEAA 390

Query: 217 MKCCAEVATDRHGCCVLQKCLSCSDHRQRDRLLTAVVCNALFLSQDQYGNYVVQFAFDLA 276
            K C E+AT RHGCCVLQ+C+S S   Q ++L+  +  N+L L+QD +GNY+VQ+  +  
Sbjct: 391 TKFCIEIATTRHGCCVLQRCVSYSVGEQHEKLVDEISRNSLLLAQDPFGNYLVQYIIEKK 450

Query: 277 RNPTAIPWVISGILKRLEGHFADLSIQKYSSNVVEKCASIGEEYLTKIIDELINDERFSQ 336
                       +L  L G++  L+ QK+ S+VVEKC     E  ++I++EL++   F  
Sbjct: 451 VGGV-------NVLFELRGNYVKLATQKFGSHVVEKCLRYYPESRSQIVNELVSVLNFGY 510

Query: 337 IMLNPYGNYAVQAVLAHSGICKGSSLHANLVAAIRPHVPLLRT 380
           ++ +PY NY +Q  L+ +   KG  + A+LV  +R +  L  T
Sbjct: 511 LLQDPYANYVIQCALSKT---KG-FVRASLVEKVRRYENLKMT 542

The following BLAST results are available for this feature:

BLAST of Carg28122 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022013.1	2.1e-229	100.00	Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022930793.1	1.1e-190	66.43	pumilio homolog 12-like [Cucurbita moschata]	[more]
KAG6588121.1	4.3e-190	66.25	Pumilio-like 12, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023530364.1	1.1e-188	66.08	uncharacterized protein LOC111792962 [Cucurbita pepo subsp. pepo]	[more]
KAG7022005.1	1.8e-188	65.96	Pumilio-like 12 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Carg28122 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVC3	5.1e-61	53.19	Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2	[more]
Q9LM20	2.6e-52	47.39	Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU...	[more]
Q9C9R6	1.3e-51	45.65	Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU...	[more]
Q1PFN9	5.4e-42	43.04	Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1	[more]
Q9LDW3	7.2e-39	39.46	Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2	[more]

BLAST of Carg28122 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWD9	5.5e-191	66.43	pumilio homolog 12-like OS=Cucurbita moschata OX=3662 GN=LOC111437164 PE=4 SV=1	[more]
A0A6J1HUY8	1.1e-188	65.19	pumilio homolog 12-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466416 P...	[more]
A0A0A0LZQ0	3.5e-129	50.36	PUM-HD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G599460 PE=4...	[more]
A0A5A7UN64	3.0e-128	50.36	Pumilio-like protein 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S4DY70	1.5e-127	50.18	uncharacterized protein LOC103492417 OS=Cucumis melo OX=3656 GN=LOC103492417 PE=...	[more]

BLAST of Carg28122 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56510.1	3.7e-62	53.19	pumilio 12	[more]
AT1G22240.1	1.8e-53	47.39	pumilio 8	[more]
AT1G78160.1	9.0e-53	45.65	pumilio 7	[more]
AT1G35730.1	3.8e-43	43.04	pumilio 9	[more]
AT4G08840.1	5.1e-40	39.46	pumilio 11	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001313	Pumilio RNA-binding repeat	SMART	SM00025	pum_5	coord: 251..287 e-value: 27.0 score: 9.6 coord: 292..326 e-value: 1.4 score: 17.9 coord: 329..363 e-value: 27.0 score: 9.5 coord: 215..250 e-value: 0.0022 score: 27.3 coord: 179..214 e-value: 6.4E-4 score: 29.0
IPR001313	Pumilio RNA-binding repeat	PFAM	PF00806	PUF	coord: 220..251 e-value: 1.5E-10 score: 40.5 coord: 296..329 e-value: 0.0025 score: 17.5 coord: 259..276 e-value: 0.004 score: 16.8 coord: 157..178 e-value: 0.24 score: 11.1 coord: 184..214 e-value: 0.0061 score: 16.2 coord: 334..354 e-value: 0.059 score: 13.1
IPR001313	Pumilio RNA-binding repeat	PROSITE	PS50302	PUM	coord: 251..287 score: 7.847311
IPR001313	Pumilio RNA-binding repeat	PROSITE	PS50302	PUM	coord: 215..250 score: 8.547253
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 153..394 e-value: 6.0E-70 score: 237.9
None	No IPR available	PANTHER	PTHR12537	RNA BINDING PROTEIN PUMILIO-RELATED	coord: 157..389
None	No IPR available	PANTHER	PTHR12537	RNA BINDING PROTEIN PUMILIO-RELATED	coord: 6..133
None	No IPR available	PANTHER	PTHR12537:SF147	PUMILIO HOMOLOG 12	coord: 6..133 coord: 157..389
IPR033133	Pumilio homology domain	PROSITE	PS50303	PUM_HD	coord: 43..394 score: 41.792961
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 157..391

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28122-RA	Carg28122-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003723	RNA binding