Carg28117 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28117
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr20: 17580 .. 18680 (+)
RNA-Seq Expression	Carg28117
Synteny	Carg28117

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGTACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

mRNA sequence

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGTACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

Coding sequence (CDS)

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGTACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

Protein sequence

MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQRDHTSI

Homology

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BLAST of Carg28117 vs. NCBI nr
Match: KAG7010206.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 735.3 bits (1897), Expect = 2.5e-208
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPF 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPF
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPF 180

Query: 181 NQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTV 240
           NQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTV
Sbjct: 181 NQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTV 240

Query: 241 RVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADES 300
           RVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADES
Sbjct: 241 RVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADES 300

Query: 301 KRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQ 360
           KRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQ
Sbjct: 301 KRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQ 360

Query: 361 RDHTSI 367
           RDHTSI
Sbjct: 361 RDHTSI 366

[Close]

BLAST of Carg28117 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 721.8 bits (1862), Expect = 2.9e-204
Identity = 364/369 (98.64%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
           FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT
Sbjct: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           VRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPADE
Sbjct: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300

Query: 301 SKRDADGGSARFM--GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSES 360
           SKRDADGGSARFM  GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGS S
Sbjct: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360

Query: 361 ESQRDHTSI 367
           ESQRDHTSI
Sbjct: 361 ESQRDHTSI 369

[Close]

BLAST of Carg28117 vs. NCBI nr
Match: XP_023512553.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 713.0 bits (1839), Expect = 1.3e-201
Identity = 359/370 (97.03%), Postives = 361/370 (97.57%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESI TRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNG+SS+NNGNNNNNSNNALE
Sbjct: 1   MDESIPTRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGVSSENNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS----RERERERERERERVIL 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS    RERERERERERERVIL
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERERVIL 180

Query: 181 INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 240
           INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA
Sbjct: 181 INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 240

Query: 241 LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300
           LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP
Sbjct: 241 LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSE 360
           ADESKRDADGGSARFM    GSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSE
Sbjct: 301 ADESKRDADGGSARFM----GSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSE 360

Query: 361 SESQRDHTSI 367
           SESQRDHTSI
Sbjct: 361 SESQRDHTSI 366

[Close]

BLAST of Carg28117 vs. NCBI nr
Match: XP_022985674.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 706.8 bits (1823), Expect = 9.5e-200
Identity = 357/375 (95.20%), Postives = 360/375 (96.00%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDADGGSARFMG-------SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNS 360
           ESKRD DGGSARFMG       SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTSI 367
           SGGSESESQRDHT I
Sbjct: 361 SGGSESESQRDHTPI 375

[Close]

BLAST of Carg28117 vs. NCBI nr
Match: XP_022985675.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 702.2 bits (1811), Expect = 2.3e-198
Identity = 353/368 (95.92%), Postives = 356/368 (96.74%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESE 360
           ESKRD DGGSARFM    GSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNSSGGSESE
Sbjct: 301 ESKRDTDGGSARFM----GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGSESE 360

Query: 361 SQRDHTSI 367
           SQRDHT I
Sbjct: 361 SQRDHTPI 364

[Close]

BLAST of Carg28117 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.5e-49
Identity = 139/377 (36.87%), Postives = 194/377 (51.46%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           M E+  TRYWCH C Q +   +  EIKCP C  GFVEEM     +D       N      
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM-----TDEEIERLTNRQ---- 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
                E   S W PI                 E   +  D++D+++  G          E
Sbjct: 61  ----PEPGFSQWNPI-----------------EHPGETMDSDDEDNDLGR---------E 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPF 180
            +  IRR RR+S T+ ++L  I   +A                    ++ER+  ILIN F
Sbjct: 121 FEGFIRRHRRAS-TLRRVLDSIHDDLAD-------------------DQERDSSILINAF 180

Query: 181 NQTIVVQGG----------ESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQ 240
           NQ + +QG           +  + N  G L +Y +G GL LLLQHLAE+DP+R GTPPA+
Sbjct: 181 NQALALQGSVLDPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAK 240

Query: 241 KEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPV 300
           KEAV+ALPTV++E     + CSVCLD+ E+  +AK+MPC+HKFHS CILPWLELHSSCPV
Sbjct: 241 KEAVEALPTVKIE---EVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPV 300

Query: 301 CRHQLPADESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSF-PWPFNGLFSS-Q 360
           CR +LP++E+K   +  +   +         +  G  +  + R ++  PW  N LFS+ +
Sbjct: 301 CRFELPSEETKDLNEPSNIGRVEDSHEEVRADGPGNVSESSNRPWAIVPW-LNELFSTRE 314

Query: 361 GNSSGGSESESQRDHTS 366
             ++GG  ++ Q  HTS
Sbjct: 361 AQNAGGVSTDQQSPHTS 314

[Close]

BLAST of Carg28117 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.7e-38
Identity = 101/242 (41.74%), Postives = 132/242 (54.55%), Query Frame = 0


Query: 133 ATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPFN------QTI-- 192
           +T+L L+ G  A   +G    S  G   + + R  + +  +    +PF       QT+  
Sbjct: 80  STLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQ-QNPQSDAFDPFTFLQNHLQTLRS 139

Query: 193 -------VVQGGESQNHNAI-GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 252
                  V++   S   N + G+ GDYF GPGL+ L+Q LAENDPNRYGTPPA K A+DA
Sbjct: 140 SGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDA 199

Query: 253 LPTVRVE---CGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRH 312
           LPTV+V          QC+VC+DEFE   + K+MPCKH FH  C+LPWLELH+SCPVCR 
Sbjct: 200 LPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRF 259

Query: 313 QLPADESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFS--FPWPFNGLFSSQGNS 354
           +LP D    D D  +      GSG G G ++G+       RFS   PWPF       G+ 
Sbjct: 260 ELPTD----DPDYENRSQGSQGSGDGQGSVEGQQTP----RFSIQLPWPFR---RQDGSG 309

[Close]

BLAST of Carg28117 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-36
Identity = 121/373 (32.44%), Postives = 164/373 (43.97%), Query Frame = 0


Query: 9   YWCHMCSQMVNPIV---DVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALESDFG- 68
           ++C+ C++ VN  +     +  CP C  GF+EE  N   +  +G  N N N+    D   
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 69  --SERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHG---DGEGNRVSSEP- 128
             S+   SL  P+L     +                D NN    G    G G+  + +P 
Sbjct: 83  TLSDPFASL-LPLLFPSSSSTT-------TSSSASIDPNNPSLSGPTRSGRGDPFAFDPF 142

Query: 129 TELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILIN 188
           T + + +   R S A I                                    E VI  N
Sbjct: 143 TFIQNHLNDLRSSGAQI------------------------------------EFVIQNN 202

Query: 189 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 248
           P            Q      ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA K A++ALP
Sbjct: 203 P----------SDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALP 262

Query: 249 TVRV---ECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQL 308
            V +          QC+VC+D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+L
Sbjct: 263 LVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 322

Query: 309 PADESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGR------RFSFPWPFNGLFSSQG 363
           P D+   +      R     SG   G+  G+ + G  R      R S PWPF       G
Sbjct: 323 PTDDPDYER---RVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA--RGPG 336

[Close]

BLAST of Carg28117 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.3e-26
Identity = 70/172 (40.70%), Postives = 95/172 (55.23%), Query Frame = 0


Query: 198 GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV---ECGDNRLQCSVC 257
           G+ GDYF GPGL+ L + L+     R G PPA + A+DALPT+++       +   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 258 LDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDADGGSARFMGS 317
            DEFE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+      +   +      
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 318 GSGSGSGEIDGRNASGTGRR--FSFPWPF--NGLFSSQGNSSGGSESESQRD 363
            S S S   + R  +G  RR  FS  WPF  +G  SS   + GG+ +    D
Sbjct: 255 RSSSSSSSSNSRE-NGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSDTSD 304

[Close]

BLAST of Carg28117 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.2e-25
Identity = 67/165 (40.61%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 201 GDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV---ECGDNRLQCSVCLDE 260
           GDYF GPGL+ L++ L+    +R G PPA K ++DALPT+++       +   C VC DE
Sbjct: 134 GDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDE 193

Query: 261 FEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDADGGSARFMGSGSG 320
           FE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+  S             S   
Sbjct: 194 FELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSS-----------SSTQS 253

Query: 321 SGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQRD 363
           S +   +GR  S     FS  WPF         SS  S ++++RD
Sbjct: 254 SQNRSTNGRENSRRRNIFSNLWPF--------RSSSSSSTQNRRD 278

[Close]

BLAST of Carg28117 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.4e-204
Identity = 364/369 (98.64%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
           FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT
Sbjct: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           VRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPADE
Sbjct: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300

Query: 301 SKRDADGGSARFM--GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSES 360
           SKRDADGGSARFM  GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGS S
Sbjct: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360

Query: 361 ESQRDHTSI 367
           ESQRDHTSI
Sbjct: 361 ESQRDHTSI 369

[Close]

BLAST of Carg28117 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 4.6e-200
Identity = 357/375 (95.20%), Postives = 360/375 (96.00%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDADGGSARFMG-------SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNS 360
           ESKRD DGGSARFMG       SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTSI 367
           SGGSESESQRDHT I
Sbjct: 361 SGGSESESQRDHTPI 375

[Close]

BLAST of Carg28117 vs. ExPASy TrEMBL
Match: A0A6J1JBZ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.1e-198
Identity = 353/368 (95.92%), Postives = 356/368 (96.74%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESE 360
           ESKRD DGGSARFM    GSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNSSGGSESE
Sbjct: 301 ESKRDTDGGSARFM----GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGSESE 360

Query: 361 SQRDHTSI 367
           SQRDHT I
Sbjct: 361 SQRDHTPI 364

[Close]

BLAST of Carg28117 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.5e-171
Identity = 320/378 (84.66%), Postives = 339/378 (89.68%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN  NNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS--------RERERVILINP 180

Query: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDADGGS-ARFM-------GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360
            DESKRD DGGS ARFM       G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360

Query: 361 GNSSGGSESESQRDHTSI 367
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSAGSASDSQRDHTSI 366

[Close]

BLAST of Carg28117 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.5e-171
Identity = 320/378 (84.66%), Postives = 339/378 (89.68%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN  NNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS--------RERERVILINP 180

Query: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDADGGS-ARFM-------GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360
            DESKRD DGGS ARFM       G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360

Query: 361 GNSSGGSESESQRDHTSI 367
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSAGSASDSQRDHTSI 366

[Close]

BLAST of Carg28117 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 9.4e-105
Identity = 216/396 (54.55%), Postives = 270/396 (68.18%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEM-GNGMSSDNNGNNNNNSNNAL 60
           M+E++A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EEM GNG      G  +   +   
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNGGGGGGRGIRDVQDS--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQ--EHGDGEGNRVSSE 120
           E+DFG++RALSLWAPILLGMM +PR RRRFR  EF E++DDN D    + DG GN  ++ 
Sbjct: 61  ETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNS 120

Query: 121 -------------------PTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGE 180
                                E +S++RRRRRSS  ILQLLQGIRAG+A     + YE  
Sbjct: 121 NNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIA-----SEYE-- 180

Query: 181 RSRERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAE 240
            S +   +  RER+RVI+INP+NQ++VV   ++QNH ++ SLGDYF+GPGLDLLLQHLAE
Sbjct: 181 -SSDNNWDNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAE 240

Query: 241 NDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCI 300
           NDPNR GTPPA+KEAV+ALPTV++      LQCSVCLD+FE   EAKEMPCKHKFH  CI
Sbjct: 241 NDPNRQGTPPARKEAVEALPTVKIM---EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCI 300

Query: 301 LPWLELHSSCPVCRHQLPA-----DESKRDAD----GGSARFMGSGS-----GSGSGEID 360
           +PWLELHSSCPVCR +LP+     DE+K D++      + R   +G+     G+     +
Sbjct: 301 VPWLELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGRE 360

[Close]

BLAST of Carg28117 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 190/386 (49.22%), Postives = 245/386 (63.47%), Query Frame = 0


Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL--ESD 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE    MS + NG ++++       E D
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEE----MSREINGGSSSSLREVQDPEID 60

Query: 65  FGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDG------------- 124
           FG++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +  DG             
Sbjct: 61  FGTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRR 120

Query: 125 -----EGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERE 184
                +G  +  +  E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S          
Sbjct: 121 HRHRQQGREIDLD-REFESILRRRRRSSATILQLLQGIRAGIAS-EYESS---------- 180

Query: 185 RERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAEND 244
                  +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+ND
Sbjct: 181 -------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADND 240

Query: 245 PNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILP 304
             R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPCKHKFH  CI+P
Sbjct: 241 SIRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVP 300

Query: 305 WLELHSSCPVCRHQLPA-DESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPW 364
           WLELHSSCPVCR++LP  DE+K +      R +     + + E D RN++ + RRFSFPW
Sbjct: 301 WLELHSSCPVCRYELPPDDETKVNPVRPRTRTLEINVSNENVEDDARNSNVSERRFSFPW 356

Query: 365 PFNGLFSSQGNSSGGSESESQRDHTS 366
           PF+GLFSS  +SS  +   S     S
Sbjct: 361 PFSGLFSSSSSSSASASGSSHLGENS 356

[Close]

BLAST of Carg28117 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 190/386 (49.22%), Postives = 245/386 (63.47%), Query Frame = 0


Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL--ESD 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE    MS + NG ++++       E D
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEE----MSREINGGSSSSLREVQDPEID 60

Query: 65  FGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDG------------- 124
           FG++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +  DG             
Sbjct: 61  FGTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRR 120

Query: 125 -----EGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERE 184
                +G  +  +  E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S          
Sbjct: 121 HRHRQQGREIDLD-REFESILRRRRRSSATILQLLQGIRAGIAS-EYESS---------- 180

Query: 185 RERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAEND 244
                  +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+ND
Sbjct: 181 -------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADND 240

Query: 245 PNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILP 304
             R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPCKHKFH  CI+P
Sbjct: 241 SIRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVP 300

Query: 305 WLELHSSCPVCRHQLPA-DESKRDADGGSARFMGSGSGSGSGEIDGRNASGTGRRFSFPW 364
           WLELHSSCPVCR++LP  DE+K +      R +     + + E D RN++ + RRFSFPW
Sbjct: 301 WLELHSSCPVCRYELPPDDETKVNPVRPRTRTLEINVSNENVEDDARNSNVSERRFSFPW 356

Query: 365 PFNGLFSSQGNSSGGSESESQRDHTS 366
           PF+GLFSS  +SS  +   S     S
Sbjct: 361 PFSGLFSSSSSSSASASGSSHLGENS 356

[Close]

BLAST of Carg28117 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.0e-86
Identity = 184/378 (48.68%), Postives = 239/378 (63.23%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           M+E++ TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEEM +    D++   +  +NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120
                    SLWAPIL+ +M +P  RRR + +E  ED+ +    E+ + +G   +    +
Sbjct: 61  ---------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE--NDLDWQ 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPF 180
           L  ++RRRRR SA +LQLLQGIRAG++  ESE++  G  +            RVILIN  
Sbjct: 121 LQEILRRRRRHSAAVLQLLQGIRAGLSV-ESESTGNGGNN----------PGRVILINTS 180

Query: 181 NQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTV 240
           NQTI VQ     +    GSLGDYF+GPG ++LLQ LAENDPNRYGTPPA+KEAV+AL TV
Sbjct: 181 NQTITVQNSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATV 240

Query: 241 RVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADES 300
           ++E     LQCSVCLD+FE+  EAK MPC HKFHS C+LPWLELHSSCPVCR+QLPADE+
Sbjct: 241 KIE---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEA 300

Query: 301 KRDADGGSARFMGSGSGS-----GSGEIDG----------------RNASGTGRRFSFPW 358
           K D+   ++   GS S S     G+   DG                 N +  G  FS PW
Sbjct: 301 KTDSVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPW 350

[Close]

BLAST of Carg28117 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.9e-66
Identity = 151/315 (47.94%), Postives = 190/315 (60.32%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL 60
           M+++  T YWCHMCS+ V P++  E IKC FC+SGFVEEM      DNN ++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLE----FDEDDDDNNDQEHGDGEGNRVS 120
                 + A SLW PIL+ MM N   +      +     +++D+D +D + GD   +   
Sbjct: 61  ------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGEI 120

Query: 121 SEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVI 180
               +L+ + R R R S  I+ LLQGIRAG+   ESEN+           +     E V+
Sbjct: 121 DITHQLEEIRRIRTRHSTAIVNLLQGIRAGLLI-ESENN----------EDNPDNSELVV 180

Query: 181 LINPFNQTI-VVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAEND-PNRYGTPPAQKEA 240
           LIN FNQ I V Q          GSLGDYF+GPG + LLQ LAEND  NRYGTPPA KEA
Sbjct: 181 LINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEA 240

Query: 241 VDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRH 300
           V+AL  V++E  D+ LQCSVCLD+FE+  EAKEMPCKHKFHS C+LPWLELHSSCPVCR+
Sbjct: 241 VEALAMVKIE--DSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRY 282

Query: 301 QLPA---DESKRDAD 306
            LP    DE K DA+
Sbjct: 301 LLPTGDDDEPKTDAE 282

The following BLAST results are available for this feature:

BLAST of Carg28117 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010206.1	2.5e-208	100.00	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022944289.1	2.9e-204	98.64	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata]	[more]
XP_023512553.1	1.3e-201	97.03	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985674.1	9.5e-200	95.20	E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima]	[more]
XP_022985675.1	2.3e-198	95.92	E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg28117 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AVN2	2.5e-49	36.87	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	9.7e-38	41.74	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.8e-36	32.44	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	1.3e-26	40.70	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	3.2e-25	40.61	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of Carg28117 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWF9	1.4e-204	98.64	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781...	[more]
A0A6J1JDY5	4.6e-200	95.20	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A6J1JBZ6	1.1e-198	95.92	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A1S3BNR7	4.5e-171	84.66	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ...	[more]
A0A5D3DDU3	4.5e-171	84.66	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Carg28117 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56340.1	9.4e-105	54.55	RING/U-box superfamily protein	[more]
AT4G26400.2	3.1e-87	49.22	RING/U-box superfamily protein	[more]
AT4G26400.1	3.1e-87	49.22	RING/U-box superfamily protein	[more]
AT1G55530.1	2.0e-86	48.68	RING/U-box superfamily protein	[more]
AT3G13430.1	3.9e-66	47.94	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 251..291 e-value: 7.0E-8 score: 42.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 250..292 e-value: 8.6E-13 score: 48.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 251..292 score: 12.40076
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 7..39 e-value: 3.3E-12 score: 46.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 215..298 e-value: 7.6E-21 score: 75.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..169
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 106..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 298..366
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 341..366
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 154..169
None	No IPR available	PANTHER	PTHR15710:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 3..309
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 3..309
None	No IPR available	CDD	cd16669	RING-H2_RNF181	coord: 250..295 e-value: 1.65102E-23 score: 89.7373
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 246..298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28117-RA	Carg28117-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016020	membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity