Carg28091 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28091
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protoporphyrinogen oxidase
Location	Carg_Chr15: 598828 .. 600786 (-)
RNA-Seq Expression	Carg28091
Synteny	Carg28091

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGATGGATGTCTCCGCTCTTATCCGTGAGGCACTGGCTGCCAGGGCTACAGAATCACATATTGTCTTAGATTACGTATCCTTCACTAATGTCTCCTTTAGTCCAGAATTTTCCAGGGCTTGCGAGTCCGAGCAGGTTAGTAAGTTTGCATCAAAGCATCAAAAGACGCCCGAGTTGAATGAGATTTTTGAGGTTGCTCCTAGTAAGAATATTAAAGCATCCTCAGCATCAGGTGAGGCCTAGCATCAAAACATGCCGAAGACTCCCATTCCCCCGAGTCTAAACACCTGAGCCCCCCTCACGACTTTTCCCTTTCTTCCTTTGGTACATAAGTACTGGATCTTCTTAAACCTCAAAGCAACTTCGTAAGAGTAGCTGAAGACTTCACTATTTTTATTACAGGTGGCCTACTTGCCTTTGTCAGTCATGATAACTACCTTCAGAAAGGAAAGTGTTAAGCGACCACTGGAGGGATTTGGAGTTCTTGTACCTTCCTTAGAGCAACAAAATGGTCTAAGAACCCTTGGTAATTTTTATTTGTTCTTTTGGATTCCTGAAAACTTACATTGTTCCTCCAAACAGACGAGTAATTCTTCTCTACAGGTACACTCTTTTCCTCCATGATGTTTCCAAATCGTGCATCAAATGATGAGTATCTGTATACTACTTTTATTGGAGGGGGTCGAAACAGGGAGCTTGCAAAGTCATCAACGTTAGTAGCGCTACTTGGTTTTCATTATTATGCAAACAAACAAAAAATATATTGCGGCCATTATCTAGTTCAACAAATTGTTTTTGTTTTATTTTTACTTCTGTTTTATTCTTGCATGTTGCAACTAAACTTTCCTTCTCTTGCTTTCCAGGGATGAGCTGAAGCAAATTGTTACATCGGATCTCAGACAGCTGTTGGGAGTTGAAGGGGAGCCCACTTTTGTCAAGTACACAAACTTCCATTTACATCGTATCACTCCTGATAGTTTTGATTAGAAATAAATGTGAAGAACTTTCACGTGTGTTCACACTTTCTTCTGGCACGTTATACGTACAATGACAGCCATTTCTACTGGAGGAAAGCATTTCCCTTATATGGGCGTAACTATGATTCAGTTGTGAAGGCAATTGAAACAATGGAAAAAAGTCTTCCTGGCTTCTTTTATGCAGGTAACTACAAGGCATCCAGAATTTGCAGAAATTATTCTTGTGTTTGAGTGTTCTGAATTAGAATTAGAACTAGAATTTGAGGTTGTTAAATAACTGTTATTCTTGATAATTAAATCTAAGAGTGATTCGTTTTTAAAATTTATTTTCAGCGCATCCTCTATTATGTGGATTTGATTATTTGTTTGATTAGCAATGTCTAACACAAGGCATGGTACTGTTGATATGAGGTGGAATAATACTAATATGAAGTTTTTATTATTATCTTTTCTAGCAGAGAAGCAAAGGAAGACTCAAATTTTAGAATATCCTTTTTCATATTATTTTAAATAATTACTAGTTAAAAAAATTTAGCTAGGTTTCTCTGATAAGCTTGTAAGTTAAGGTTAATATAATCATCAAACTTTCTTTCTTAAATCATATTATACCATTAACTTTTTTGTCATCCTCATATCCGTTTTTCGTTTTTAGGTTTACATTACTTACATCTAGCTTCGTACATATTTGTTCAAATCCTATGACGTGATGTGATGGGCGTATTCTTACTGTTTTGTTACATTCAATTTTCCTATCCCCGAGTAATATGCACTATTTCATTTCTAAATCTTGCATACTTTTAGTTTCTTGTGAGTTGTGGATTTTCAACCTTATGAATCACGTACCTTGTAGGTAACCACAGGGATGGTTTGTCTGTTGGAAAATCTATAGCCTCCGGATGCAAAGCTGCTGATCTTGTCATTTCTTATCTGGAGTCTTCTACTGATCTGCCGTGTGCTGAACAACGAGAGTTGAAAAAATGA

mRNA sequence

CTTGATGGATGTCTCCGCTCTTATCCGTGAGGCACTGGCTGCCAGGGCTACAGAATCACATATTGTCTTAGATTACGTATCCTTCACTAATGTCTCCTTTAGTCCAGAATTTTCCAGGGCTTGCGAGTCCGAGCAGGTTAGTAAGTTTGCATCAAAGCATCAAAAGACGCCCGAGTTGAATGAGATTTTTGAGGTTGCTCCTAGTAAGAATATTAAAGCATCCTCAGCATCAGGTGGCCTACTTGCCTTTGTCAGTCATGATAACTACCTTCAGAAAGGAAAGTGTTAAGCGACCACTGGAGGGATTTGGAGTTCTTGTACCTTCCTTAGAGCAACAAAATGGTCTAAGAACCCTTGGTACACTCTTTTCCTCCATGATGTTTCCAAATCGTGCATCAAATGATGAGTATCTGTATACTACTTTTATTGGAGGGGGTCGAAACAGGGAGCTTGCAAAGTCATCAACGGATGAGCTGAAGCAAATTGTTACATCGGATCTCAGACAGCTGTTGGGAGTTGAAGGGGAGCCCACTTTTGTCAACCATTTCTACTGGAGGAAAGCATTTCCCTTATATGGGCGTAACTATGATTCAGTTGTGAAGGCAATTGAAACAATGGAAAAAAGTCTTCCTGGCTTCTTTTATGCAGGTAACCACAGGGATGGTTTGTCTGTTGGAAAATCTATAGCCTCCGGATGCAAAGCTGCTGATCTTGTCATTTCTTATCTGGAGTCTTCTACTGATCTGCCGTGTGCTGAACAACGAGAGTTGAAAAAATGA

Coding sequence (CDS)

ATGAGATTTTTGAGGTTGCTCCTAGTAAGAATATTAAAGCATCCTCAGCATCAGGTGGCCTACTTGCCTTTGTCAGTCATGATAACTACCTTCAGAAAGGAAAGTGTTAAGCGACCACTGGAGGGATTTGGAGTTCTTGTACCTTCCTTAGAGCAACAAAATGGTCTAAGAACCCTTGGTACACTCTTTTCCTCCATGATGTTTCCAAATCGTGCATCAAATGATGAGTATCTGTATACTACTTTTATTGGAGGGGGTCGAAACAGGGAGCTTGCAAAGTCATCAACGGATGAGCTGAAGCAAATTGTTACATCGGATCTCAGACAGCTGTTGGGAGTTGAAGGGGAGCCCACTTTTGTCAACCATTTCTACTGGAGGAAAGCATTTCCCTTATATGGGCGTAACTATGATTCAGTTGTGAAGGCAATTGAAACAATGGAAAAAAGTCTTCCTGGCTTCTTTTATGCAGGTAACCACAGGGATGGTTTGTCTGTTGGAAAATCTATAGCCTCCGGATGCAAAGCTGCTGATCTTGTCATTTCTTATCTGGAGTCTTCTACTGATCTGCCGTGTGCTGAACAACGAGAGTTGAAAAAATGA

Protein sequence

MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQRELKK

Homology

[Close]

BLAST of Carg28091 vs. NCBI nr
Match: XP_022938677.1 (protoporphyrinogen oxidase, chloroplastic/mitochondrial-like [Cucurbita moschata] >KAG7015970.1 Protoporphyrinogen oxidase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 398.3 bits (1022), Expect = 3.9e-107
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG 60
           MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG
Sbjct: 1   MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG 60

Query: 61  TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV 120
           TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV
Sbjct: 61  TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV 120

Query: 121 NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI 180
           NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI
Sbjct: 121 NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI 180

Query: 181 SYLESSTDLPCAEQRELKK 200
           SYLESSTDLPCAEQRELKK
Sbjct: 181 SYLESSTDLPCAEQRELKK 199

[Close]

BLAST of Carg28091 vs. NCBI nr
Match: XP_022992609.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 175/182 (96.15%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAY+PLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 324 EVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 383

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 384 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 443

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SV+KAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD PCAEQ+EL
Sbjct: 444 SVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDQPCAEQQEL 503

Query: 198 KK 200
           KK
Sbjct: 504 KK 505

[Close]

BLAST of Carg28091 vs. NCBI nr
Match: XP_022992607.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X1 [Cucurbita maxima] >XP_022992608.1 protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 175/182 (96.15%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAY+PLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 329 EVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 388

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 389 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 448

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SV+KAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD PCAEQ+EL
Sbjct: 449 SVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDQPCAEQQEL 508

Query: 198 KK 200
           KK
Sbjct: 509 KK 510

[Close]

BLAST of Carg28091 vs. NCBI nr
Match: XP_022938652.1 (protoporphyrinogen oxidase, chloroplastic/mitochondrial isoform X8 [Cucurbita moschata])

HSP 1 Score: 355.1 bits (910), Expect = 3.8e-94
Identity = 176/182 (96.70%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 329 EVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 388

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 389 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 448

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESST  PCAEQ+EL
Sbjct: 449 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTHQPCAEQQEL 508

Query: 198 KK 200
           KK
Sbjct: 509 KK 510

[Close]

BLAST of Carg28091 vs. NCBI nr
Match: XP_022938645.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 355.1 bits (910), Expect = 3.8e-94
Identity = 176/182 (96.70%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 337 EVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 396

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 397 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 456

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESST  PCAEQ+EL
Sbjct: 457 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTHQPCAEQQEL 516

Query: 198 KK 200
           KK
Sbjct: 517 KK 518

[Close]

BLAST of Carg28091 vs. ExPASy Swiss-Prot
Match: Q94IG7 (Protoporphyrinogen oxidase 2 OS=Spinacia oleracea OX=3562 GN=POX2 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.1e-75
Identity = 136/169 (80.47%), Postives = 154/169 (91.12%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +V+ LPLSV+ITTF+K +VKRPLEGFGVLVPS EQ NGL+TLGTLFSSMMFP+RA +D Y
Sbjct: 356 EVSCLPLSVIITTFKKTNVKRPLEGFGVLVPSNEQHNGLKTLGTLFSSMMFPDRAPSDVY 415

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTF+GG RNRELAK+STDELKQIV+SDL+QLLG EGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 416 LYTTFVGGSRNRELAKASTDELKQIVSSDLQQLLGTEGEPTFVNHFYWSKAFPLYGRNYD 475

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESS 187
           SV++AIE ME+ LPG FYAGNH+ GLSVGKSIASG KAA+L ISYLES+
Sbjct: 476 SVLRAIEKMERDLPGLFYAGNHKGGLSVGKSIASGYKAAELAISYLESN 524

[Close]

BLAST of Carg28091 vs. ExPASy Swiss-Prot
Match: Q8S9J1 (Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.0e-73
Identity = 130/171 (76.02%), Postives = 154/171 (90.06%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           ++ Y+PLSV+ITTF KE VKRPLEGFGVL+PS EQ++G +TLGTLFSSMMFP+R+ +D +
Sbjct: 330 EINYMPLSVLITTFTKEKVKRPLEGFGVLIPSKEQKHGFKTLGTLFSSMMFPDRSPSDVH 389

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RN+ELAK+STDELKQ+VTSDL++LLGVEGEP  VNH+YWRKAFPLY  +YD
Sbjct: 390 LYTTFIGGSRNQELAKASTDELKQVVTSDLQRLLGVEGEPVSVNHYYWRKAFPLYDSSYD 449

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD 189
           SV++AI+ ME  LPGFFYAGNHR GLSVGKSIASGCKAADLVISYLES ++
Sbjct: 450 SVMEAIDKMENDLPGFFYAGNHRGGLSVGKSIASGCKAADLVISYLESCSN 500

[Close]

BLAST of Carg28091 vs. ExPASy Swiss-Prot
Match: O24164 (Protoporphyrinogen oxidase, mitochondrial OS=Nicotiana tabacum OX=4097 GN=PPXII PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.0e-73
Identity = 133/172 (77.33%), Postives = 154/172 (89.53%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +V Y+PLSV+ITTF++E+VK PLEGFGVLVPS EQQ+GL+TLGTLFSSMMFP+RA N+ Y
Sbjct: 328 EVDYVPLSVVITTFKRENVKYPLEGFGVLVPSKEQQHGLKTLGTLFSSMMFPDRAPNNVY 387

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTF+GG RNRELAK+S  ELK+IVTSDL+QLLG EGEPT+VNH YW KAFPLYG NYD
Sbjct: 388 LYTTFVGGSRNRELAKASRTELKEIVTSDLKQLLGAEGEPTYVNHLYWSKAFPLYGHNYD 447

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLES-STD 189
           SV+ AI+ MEK+LPG FYAGNHR GLSVGK+++SGC AADLVISYLES STD
Sbjct: 448 SVLDAIDKMEKNLPGLFYAGNHRGGLSVGKALSSGCNAADLVISYLESVSTD 499

[Close]

BLAST of Carg28091 vs. ExPASy Swiss-Prot
Match: O24163 (Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 52/173 (30.06%), Postives = 89/173 (51.45%), Query Frame = 0


Query: 21  YLPLSVMITTFRKESVK------RPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASN 80
           Y P+  +  T+ +E+++        L+GFG L P   +  G+ TLGT++SS +FPNRA  
Sbjct: 373 YPPVGAVTITYPQEAIRDERLVDGELKGFGQLHP---RTQGVETLGTIYSSSLFPNRAPK 432

Query: 81  DEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLL--GVEGEPTFVNHFYWRKAFPLY 140
              L   +IGG +N E+   +  +L ++V  DLR++L      +P  V    W +A P +
Sbjct: 433 GRVLLLNYIGGAKNPEILSKTESQLVEVVDRDLRKMLIKPKAQDPLVVGVRVWPQAIPQF 492

Query: 141 -GRNYDSVVKAIETM-EKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYL 184
              + D++  A   M +  L G F  GN+  G+++G+ +    + A  V  +L
Sbjct: 493 LVGHLDTLSTAKAAMNDNGLEGLFLGGNYVSGVALGRCVEGAYEVASEVTGFL 542

[Close]

BLAST of Carg28091 vs. ExPASy Swiss-Prot
Match: Q9AR38 (Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PPOX1 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.9e-14
Identity = 48/173 (27.75%), Postives = 88/173 (50.87%), Query Frame = 0


Query: 21  YLPLSVMITTFRKESVKR------PLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASN 80
           Y P++ +  ++ KE++++       L+GFG L P   +  G+ TLGT++SS +FPNRA  
Sbjct: 361 YPPVAAVTVSYPKEAIRKECLIDGELQGFGQLHP---RSQGVETLGTIYSSSLFPNRAPA 420

Query: 81  DEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLL--GVEGEPTFVNHFYWRKAFPLY 140
              L   +IGG  N  +   +  EL + V  DLR++L      +P  +    W +A P +
Sbjct: 421 GRVLLLNYIGGSTNTGIVSKTESELVEAVDRDLRKMLINPKAVDPLVLGVRVWPQAIPQF 480

Query: 141 GRNYDSVVKAIETM--EKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYL 184
              +   ++A ++   +    G F  GN+  G+++G+ +    ++A  +  YL
Sbjct: 481 LIGHLDHLEAAKSALGKGGYDGLFLGGNYVAGVALGRCVEGAYESASQISDYL 530

[Close]

BLAST of Carg28091 vs. ExPASy TrEMBL
Match: A0A6J1FER9 (protoporphyrinogen oxidase, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444831 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.9e-107
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG 60
           MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG
Sbjct: 1   MRFLRLLLVRILKHPQHQVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLG 60

Query: 61  TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV 120
           TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV
Sbjct: 61  TLFSSMMFPNRASNDEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFV 120

Query: 121 NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI 180
           NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI
Sbjct: 121 NHFYWRKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVI 180

Query: 181 SYLESSTDLPCAEQRELKK 200
           SYLESSTDLPCAEQRELKK
Sbjct: 181 SYLESSTDLPCAEQRELKK 199

[Close]

BLAST of Carg28091 vs. ExPASy TrEMBL
Match: A0A6J1JQC4 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 175/182 (96.15%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAY+PLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 329 EVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 388

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 389 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 448

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SV+KAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD PCAEQ+EL
Sbjct: 449 SVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDQPCAEQQEL 508

Query: 198 KK 200
           KK
Sbjct: 509 KK 510

[Close]

BLAST of Carg28091 vs. ExPASy TrEMBL
Match: A0A6J1JY02 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 175/182 (96.15%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAY+PLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 324 EVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 383

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 384 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 443

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SV+KAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD PCAEQ+EL
Sbjct: 444 SVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDQPCAEQQEL 503

Query: 198 KK 200
           KK
Sbjct: 504 KK 505

[Close]

BLAST of Carg28091 vs. ExPASy TrEMBL
Match: A0A6J1FKC6 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.9e-94
Identity = 176/182 (96.70%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 337 EVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 396

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 397 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 456

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESST  PCAEQ+EL
Sbjct: 457 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTHQPCAEQQEL 516

Query: 198 KK 200
           KK
Sbjct: 517 KK 518

[Close]

BLAST of Carg28091 vs. ExPASy TrEMBL
Match: A0A6J1FDR3 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.9e-94
Identity = 176/182 (96.70%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           +VAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY
Sbjct: 332 EVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 391

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYW KAFPLYGRNYD
Sbjct: 392 LYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYD 451

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTDLPCAEQREL 197
           SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESST  PCAEQ+EL
Sbjct: 452 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTHQPCAEQQEL 511

Query: 198 KK 200
           KK
Sbjct: 512 KK 513

[Close]

BLAST of Carg28091 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-74
Identity = 130/171 (76.02%), Postives = 154/171 (90.06%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           ++ Y+PLSV+ITTF KE VKRPLEGFGVL+PS EQ++G +TLGTLFSSMMFP+R+ +D +
Sbjct: 330 EINYMPLSVLITTFTKEKVKRPLEGFGVLIPSKEQKHGFKTLGTLFSSMMFPDRSPSDVH 389

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RN+ELAK+STDELKQ+VTSDL++LLGVEGEP  VNH+YWRKAFPLY  +YD
Sbjct: 390 LYTTFIGGSRNQELAKASTDELKQVVTSDLQRLLGVEGEPVSVNHYYWRKAFPLYDSSYD 449

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD 189
           SV++AI+ ME  LPGFFYAGNHR GLSVGKSIASGCKAADLVISYLES ++
Sbjct: 450 SVMEAIDKMENDLPGFFYAGNHRGGLSVGKSIASGCKAADLVISYLESCSN 500

[Close]

BLAST of Carg28091 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-74
Identity = 130/171 (76.02%), Postives = 154/171 (90.06%), Query Frame = 0


Query: 18  QVAYLPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEY 77
           ++ Y+PLSV+ITTF KE VKRPLEGFGVL+PS EQ++G +TLGTLFSSMMFP+R+ +D +
Sbjct: 300 EINYMPLSVLITTFTKEKVKRPLEGFGVLIPSKEQKHGFKTLGTLFSSMMFPDRSPSDVH 359

Query: 78  LYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGRNYD 137
           LYTTFIGG RN+ELAK+STDELKQ+VTSDL++LLGVEGEP  VNH+YWRKAFPLY  +YD
Sbjct: 360 LYTTFIGGSRNQELAKASTDELKQVVTSDLQRLLGVEGEPVSVNHYYWRKAFPLYDSSYD 419

Query: 138 SVVKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKAADLVISYLESSTD 189
           SV++AI+ ME  LPGFFYAGNHR GLSVGKSIASGCKAADLVISYLES ++
Sbjct: 420 SVMEAIDKMENDLPGFFYAGNHRGGLSVGKSIASGCKAADLVISYLESCSN 470

[Close]

BLAST of Carg28091 vs. TAIR 10
Match: AT4G01690.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-13
Identity = 50/173 (28.90%), Postives = 88/173 (50.87%), Query Frame = 0


Query: 21  YLPLSVMITTFRKESVKR------PLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASN 80
           Y P++ +  ++ KE+++        L+GFG L P   +  G+ TLGT++SS +FPNRA  
Sbjct: 362 YPPVAAVSISYPKEAIRTECLIDGELKGFGQLHP---RTQGVETLGTIYSSSLFPNRAPP 421

Query: 81  DEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLL--GVEGEPTFVNHFYWRKAFPLY 140
              L   +IGG  N  +   S  EL + V  DLR++L      +P  +    W +A P +
Sbjct: 422 GRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVRVWPQAIPQF 481

Query: 141 -GRNYDSVVKAIETMEKS-LPGFFYAGNHRDGLSVGKSIASGCKAADLVISYL 184
              ++D +  A  ++  S   G F  GN+  G+++G+ +    + A  V +++
Sbjct: 482 LVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFM 531

[Close]

BLAST of Carg28091 vs. TAIR 10
Match: AT4G01690.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 55.8 bits (133), Expect = 4.5e-08
Identity = 45/170 (26.47%), Postives = 74/170 (43.53%), Query Frame = 0


Query: 21  YLPLSVMITTFRKESVKR------PLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASN 80
           Y P++ +  ++ KE+++        L+GFG L P   +  G+ TLGT++SS +FPNRA  
Sbjct: 362 YPPVAAVSISYPKEAIRTECLIDGELKGFGQLHP---RTQGVETLGTIYSSSLFPNRAPP 421

Query: 81  DEYLYTTFIGGGRNRELAKSSTDELKQIVTSDLRQLLGVEGEPTFVNHFYWRKAFPLYGR 140
              L   +IGG  N  +   S  EL                E   V HF           
Sbjct: 422 GRILLLNYIGGSTNTGILSKSEGELV---------------EAFLVGHF----------- 481

Query: 141 NYDSVVKAIETMEKS-LPGFFYAGNHRDGLSVGKSIASGCKAADLVISYL 184
             D +  A  ++  S   G F  GN+  G+++G+ +    + A  V +++
Sbjct: 482 --DILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFM 500

The following BLAST results are available for this feature:

BLAST of Carg28091 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022938677.1	3.9e-107	100.00	protoporphyrinogen oxidase, chloroplastic/mitochondrial-like [Cucurbita moschata...	[more]
XP_022992609.1	2.2e-94	96.15	protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X2 [Cucur...	[more]
XP_022992607.1	2.2e-94	96.15	protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X1 [Cucur...	[more]
XP_022938652.1	3.8e-94	96.70	protoporphyrinogen oxidase, chloroplastic/mitochondrial isoform X8 [Cucurbita mo...	[more]
XP_022938645.1	3.8e-94	96.70	protoporphyrinogen oxidase 2, chloroplastic/mitochondrial isoform X2 [Cucurbita ...	[more]

BLAST of Carg28091 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IG7	4.1e-75	80.47	Protoporphyrinogen oxidase 2 OS=Spinacia oleracea OX=3562 GN=POX2 PE=1 SV=1	[more]
Q8S9J1	3.0e-73	76.02	Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial OS=Arabidopsis thalian...	[more]
O24164	3.0e-73	77.33	Protoporphyrinogen oxidase, mitochondrial OS=Nicotiana tabacum OX=4097 GN=PPXII ...	[more]
O24163	2.2e-15	30.06	Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI P...	[more]
Q9AR38	6.9e-14	27.75	Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of Carg28091 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FER9	1.9e-107	100.00	protoporphyrinogen oxidase, chloroplastic/mitochondrial-like OS=Cucurbita moscha...	[more]
A0A6J1JQC4	1.1e-94	96.15	Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1	[more]
A0A6J1JY02	1.1e-94	96.15	Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1	[more]
A0A6J1FKC6	1.9e-94	96.70	Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV...	[more]
A0A6J1FDR3	1.9e-94	96.70	Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV...	[more]

BLAST of Carg28091 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14220.1	2.1e-74	76.02	Flavin containing amine oxidoreductase family	[more]
AT5G14220.2	2.1e-74	76.02	Flavin containing amine oxidoreductase family	[more]
AT4G01690.1	2.1e-13	28.90	Flavin containing amine oxidoreductase family	[more]
AT4G01690.2	4.5e-08	26.47	Flavin containing amine oxidoreductase family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002937	Amine oxidase	PFAM	PF01593	Amino_oxidase	coord: 18..180 e-value: 8.3E-23 score: 81.4
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 126..188 e-value: 1.8E-5 score: 27.3
None	No IPR available	GENE3D	3.90.660.20	Protoporphyrinogen oxidase, mitochondrial; domain 2	coord: 10..125 e-value: 1.1E-43 score: 151.0
None	No IPR available	PANTHER	PTHR42923	PROTOPORPHYRINOGEN OXIDASE	coord: 18..184
None	No IPR available	PANTHER	PTHR42923:SF3	PROTOPORPHYRINOGEN OXIDASE	coord: 18..184
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 21..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28091-RA	Carg28091-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004729	oxygen-dependent protoporphyrinogen oxidase activity
molecular_function	GO:0016491	oxidoreductase activity