Carg28083 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28083
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mycolic acid cyclopropane synthase
Location	Carg_Chr14: 9364870 .. 9367579 (+)
RNA-Seq Expression	Carg28083
Synteny	Carg28083

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATCTACTTTTTGTTGGGGATTGTGGTCTCCTCCACTGTTTACCGCAACTATTGCATCTGCAAAGTATTTCTTTCAGCATACTTTAAGAAAGAACACCATTGCACAAGCTCGTAAAAATATTTCTCGTCATTACGATCTCGTAGTATATGGATGCATCAGTTTCATAGTCTTTTTCTTTTCATCTTTAGATTGATGATATCTCTCCAATCTTATTCGTATTCAATCAAAAGATAACTAGAATAATGATGAAAAATAACAAACAAAAAAAATGTAAGATAACTCAAATAAGTATGAGATTGTAGCGTTTGAGTAAAGCAGTGTTACATAAATAAATTGAGACCAGAAGTGATCCAAAGATATAAAATTATAGTTAAAAAAAAGTTTACAAGAATTTATAGCTCCTACCCATATTACTAATAAAGTGAACATTTCTTTTCGTTTGGAAACTTAATCATAGGAACTTCAAAATTAAACGTGTTGAATCTCCTTAAAATTATAGATTTCGAATCTTGAGGTGAAGAATTATAGAACAAATCATCGTATCAACCTTTCAATATAATTACTAATATGCCCTCAATATCCAATAACATTTCTAATTATTCTATCTTAATTCCATTTGTATCAAACTATTCTCTCCTATTGAATAATTGATCTGGTTGTATAGAGCAATGAACTTTTCTCTCTCTTCTTGGATGATACAATGATGTACTCATGTGCCATCATTAAGGTTCCTATAACCAACTTTTATTTATTAAAAATTTGATAATCTATATGATGATTTGCATTATTTTCTTAACAAAGAGTTGGTAGATGAAGATTTGAGGATTGCACAATTGAGGAAAATCTCTGTTCTTATTGAGAAGGTGATTATTATATTATTACACAATTAAACATATTTTACATTACCTAATCCTTACTTCTTAATAGGCAAGCATCAACGAAAAGCATAAGGTTTTAGATATTGGGTATGGTTGGGGAACTTTAGCTATTGAAATAGTTAAAGCAAACTGGATGTCACTGGCATTACTTTGTCCAAACAACAATTGAAATATGCCCAAAAAATGCCCAAAAAAGGGTTCAAGATCTTGGCCTTCAGGTAATATCTCTTTCTTATGCGCCTATTTTGCTTCTTTCGATTGTTGTTTGAGTTTGTGTAGTTTCTTTGTTGCTCTTTGTTGCTTAACAATCAAGAGCTAAAGCTTATCATGAAGTACCATCCTTGTTACTTAATAATCAAGTGCTAACCTTGAGATCATATTGAATTAGGGTAGCATTTGGTTACTTAACAATCAAGGGCTAACCTAAGCACAGCTTGAAGCTTAAGGTGTGGTCACACCTTGATTATAATACTTGGTAACATTCACTATCATAAAGTGAGCTTAAGTCATATTAAACTTCTAGACCTCCATAAATCTTGGTTAACCATTAGAGGCACTTTAGTCATTTCTCTTAAGCCTTAGCTTATACCCCTAACTTGATTTAGATTCTAGACTTGATCCAAACATCTTGAAATATTACATAACCCTTCATGTCCTTGTATATAGTCATTACTTAAAATATTGGATCATTGGGAGGGTATTTGGACCTAAAATATTTTAAAGGAACATTAGGGACATTTAGGTCATTTTACCACCTTAAACTTAGTTCGAACCCCAAGCGTGATCAATAGTTCTAAAAATTTATATAATTCTTTCACATAGTAAAATAAGCTTCATATAAAGTTTCATGATATTAGGAGATCGTTGGGTAATAAATAAAAGGTAAGTTATTTTAGGAGTATTATAGTCATTTTACACCATTACTTGGTTTCATTACTAATCATTTTGATCCAACCTTCTTGTAGGATCAAATTGAGTTTCTTCTTTGTGACAGAATTGTCAAGTACTACTAAATATGATAGGATCCTATCCTGGTGAGTTTTCGAATGAAATAAAGCTATTAATAATAATATGTATATATATTATTTGATTAAATTATAGATTTAATATCTAAACTTGCTATATATATTATTTGTTCAATGTCATCGAAACTTTCATACTAAACTTTTAGTTATAATTTCTTAAGTTATAATTTATTAGACATAAAAATTAAAAAATTAAACTAAATTTATAATTTAACGTTGGCAGCTAGATGATAAAAGCAGTTGGCCACGAATTCATGGGAGATTTCTTTGGTTCTTATGAATCAGTGTCGAGGAAAATGGTCTTGTTGTTCTACATCTTCAATTAATTTTTTTAAAAAAACTTTTTAGTGATAATTAATTTTTTTTAATTATTCGTTATTTATTTATTATTATTATTATTATTATTATTATTATTATTTTGCATTAATTCATATCCGTGCCAGACGAGTCTTATGAGGAGCATCCGTTAAGTTTTGGTTTTATAAAAGAATATATATTTCAAGGAGGATGTCTTCAATCATTAAGTAGAATAACAACGGGCATGGCTGCTGCATCAAGGCTTTGGTAAATAATTCATTTATGTTCTTCATTTTCAACAATAATTATTAACAAATAATGAAAAAAAATCCTTTGGGTACAGTGTGGAGCACTTAGAAAATATTGGAATTCATTACTATCGAACATTGAGGTGTTTGGATAAAGAATTTCTTGAACAATCAAAGGTAAGAAACCAAACCATAATTATAATAAATGGTTTCAAATTCCAATCTAATTTATATTTTGTTTCAGCAAAATACTTGA

mRNA sequence

ATGGTGATCTACTTTTTGTTGGGGATTGTGGTCTCCTCCACTGTTTACCGCAACTATTGCATCTGCAAACATACTTTAAGAAAGAACACCATTGCACAAGCTCGTAAAAATATTTCTCGTCATTACGATCTCGTAAGCAATGAACTTTTCTCTCTCTTCTTGGATGATACAATGATGTACTCATGTGCCATCATTAAGTTGGTAGATGAAGATTTGAGGATTGCACAATTGAGGAAAATCTCTGTTCTTATTGAGAAGGCAAGCATCAACGAAAAGCATAAGTTAAAGCAAACTGGATGTCACTGGCATTACTTTGTCCAAACAACAATTGAAATATGCCCAAAAAATGCCCAAAAAAGGGTTCAAGATCTTGGCCTTCAGATGATAAAAGCAGTTGGCCACGAATTCATGGGAGATTTCTTTGGTTCTTATGAATCAGTGTCGAGGAAAATGGTCTTTGTGGAGCACTTAGAAAATATTGGAATTCATTACTATCGAACATTGAGGTGTTTGGATAAAGAATTTCTTGAACAATCAAAGCAAAATACTTGA

Coding sequence (CDS)

ATGGTGATCTACTTTTTGTTGGGGATTGTGGTCTCCTCCACTGTTTACCGCAACTATTGCATCTGCAAACATACTTTAAGAAAGAACACCATTGCACAAGCTCGTAAAAATATTTCTCGTCATTACGATCTCGTAAGCAATGAACTTTTCTCTCTCTTCTTGGATGATACAATGATGTACTCATGTGCCATCATTAAGTTGGTAGATGAAGATTTGAGGATTGCACAATTGAGGAAAATCTCTGTTCTTATTGAGAAGGCAAGCATCAACGAAAAGCATAAGTTAAAGCAAACTGGATGTCACTGGCATTACTTTGTCCAAACAACAATTGAAATATGCCCAAAAAATGCCCAAAAAAGGGTTCAAGATCTTGGCCTTCAGATGATAAAAGCAGTTGGCCACGAATTCATGGGAGATTTCTTTGGTTCTTATGAATCAGTGTCGAGGAAAATGGTCTTTGTGGAGCACTTAGAAAATATTGGAATTCATTACTATCGAACATTGAGGTGTTTGGATAAAGAATTTCTTGAACAATCAAAGCAAAATACTTGA

Protein sequence

MVIYFLLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQMIKAVGHEFMGDFFGSYESVSRKMVFVEHLENIGIHYYRTLRCLDKEFLEQSKQNT

Homology

[Close]

BLAST of Carg28083 vs. NCBI nr
Match: KAG7018231.1 (ufaA1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.0 bits (946), Expect = 2.4e-98
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MVIYFLLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMY 60
           MVIYFLLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMY
Sbjct: 1   MVIYFLLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMY 60

Query: 61  SCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHWHYFVQTTIEICPKNAQKR 120
           SCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHWHYFVQTTIEICPKNAQKR
Sbjct: 61  SCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHWHYFVQTTIEICPKNAQKR 120

Query: 121 VQDLGLQMIKAVGHEFMGDFFGSYESVSRKMVFVEHLENIGIHYYRTLRCLDKEFLEQSK 180
           VQDLGLQMIKAVGHEFMGDFFGSYESVSRKMVFVEHLENIGIHYYRTLRCLDKEFLEQSK
Sbjct: 121 VQDLGLQMIKAVGHEFMGDFFGSYESVSRKMVFVEHLENIGIHYYRTLRCLDKEFLEQSK 180

Query: 181 QNT 184
           QNT
Sbjct: 181 QNT 183

[Close]

BLAST of Carg28083 vs. NCBI nr
Match: XP_022973880.1 (uncharacterized protein LOC111472470 isoform X2 [Cucurbita maxima])

HSP 1 Score: 176.8 bits (447), Expect = 1.7e-40
Identity = 121/250 (48.40%), Postives = 132/250 (52.80%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HTLRKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI K  DEDLRIAQLRKIS+
Sbjct: 142 QHTLRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISI 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDLGLQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLGLQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 181
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCESVLEDNGLLVLQFISM 321

[Close]

BLAST of Carg28083 vs. NCBI nr
Match: XP_022974371.1 (uncharacterized protein LOC111473008 isoform X1 [Cucurbita maxima])

HSP 1 Score: 175.6 bits (444), Expect = 3.8e-40
Identity = 120/250 (48.00%), Postives = 132/250 (52.80%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HTLRKNTIAQARKNISRHYDL +NELFSLFLDDTMMYSCAI K  DEDLRIAQLRKIS+
Sbjct: 142 QHTLRKNTIAQARKNISRHYDL-NNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISI 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDLGLQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLGLQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 181
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCESVLEDNGLLVLQFISM 321

[Close]

BLAST of Carg28083 vs. NCBI nr
Match: XP_022974792.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111473548 [Cucurbita maxima])

HSP 1 Score: 175.6 bits (444), Expect = 3.8e-40
Identity = 124/265 (46.79%), Postives = 135/265 (50.94%), Query Frame = 0


Query: 6   LLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAII 65
           L    ++ST Y      +HTLRKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI 
Sbjct: 53  LFTATIASTKY----FFQHTLRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIF 112

Query: 66  KLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHW-------------HYFVQTTIEI 125
           K  DEDLRIAQLRKIS+LI+KA IN+KHK+   GC W             H    T  + 
Sbjct: 113 KREDEDLRIAQLRKISILIKKARINKKHKVLDMGCGWGTLAIEIVKQXGCHCTGITLSKQ 172

Query: 126 CPKNAQKRVQDLGLQ--------------------------MIKAVGHEFMGDFFGSYES 181
             K AQKRVQDLGLQ                          MI+AVGHEFM DFFGS ES
Sbjct: 173 QLKYAQKRVQDLGLQDHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCES 232

[Close]

BLAST of Carg28083 vs. NCBI nr
Match: XP_022932946.1 (uncharacterized protein LOC111439562 isoform X1 [Cucurbita moschata])

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-38
Identity = 116/245 (47.35%), Postives = 126/245 (51.43%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HT RKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI K  DEDLRIAQLRKISV
Sbjct: 142 RHTSRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISV 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDL LQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLALQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 177
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCRMIEAVGHEFMEDFFGSCESVLQDNGLLVLQFISV 321

[Close]

BLAST of Carg28083 vs. ExPASy Swiss-Prot
Match: O53732 (Tuberculostearic acid methyltransferase UfaA1 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ufaA1 PE=1 SV=3)

HSP 1 Score: 56.2 bits (134), Expect = 4.4e-07
Identity = 52/155 (33.55%), Postives = 74/155 (47.74%), Query Frame = 0


Query: 33  QARKNISRHYDLVSNELFSLFLDDTMMYSCAII-KLVDE------DLRIAQLRKISVLIE 92
           QAR+NI+ HYDL SN+LF+ FLD+TM YSCA+   L+ +      +L  AQ RKI  L++
Sbjct: 144 QARRNIAVHYDL-SNDLFAAFLDETMTYSCAMFTDLLAQPTPAWTELAAAQRRKIDRLLD 203

Query: 93  KASINEKHKLKQTGCHWHYFVQTTIEICPKNAQK-----------RVQDLGLQMIKAV-- 152
            A + +   + + G  W        E+C + A +             Q L  Q + A   
Sbjct: 204 VAGVQQGSHVLEIGTGWG-------ELCIRAAARGAHIRSVTLSVEQQRLARQRVAAAGF 263

Query: 153 GHEF------MGDFFGSYESVSRKMVFVEHLENIG 162
           GH          D  G Y+SV    V VE +E +G
Sbjct: 264 GHRVEIDLCDYRDVDGQYDSV----VSVEMIEAVG 286

[Close]

BLAST of Carg28083 vs. ExPASy Swiss-Prot
Match: P9WPB2 (Cyclopropane mycolic acid synthase 3 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pcaA PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.9e-06
Identity = 29/75 (38.67%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 37  NISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLK 96
           N+  HYDL S++ F LFLD T  YSCA  +  D  L+ AQ+ KI + + K ++     L 
Sbjct: 11  NVQAHYDL-SDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKLNLEPGMTLL 70

Query: 97  QTGCHWHYFVQTTIE 112
             GC W   ++  IE
Sbjct: 71  DIGCGWGATMRRAIE 84

[Close]

BLAST of Carg28083 vs. ExPASy Swiss-Prot
Match: P9WPB3 (Cyclopropane mycolic acid synthase 3 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pcaA PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.9e-06
Identity = 29/75 (38.67%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 37  NISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLK 96
           N+  HYDL S++ F LFLD T  YSCA  +  D  L+ AQ+ KI + + K ++     L 
Sbjct: 11  NVQAHYDL-SDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKLNLEPGMTLL 70

Query: 97  QTGCHWHYFVQTTIE 112
             GC W   ++  IE
Sbjct: 71  DIGCGWGATMRRAIE 84

[Close]

BLAST of Carg28083 vs. ExPASy Swiss-Prot
Match: A5U866 (Cyclopropane mycolic acid synthase 1 OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) OX=419947 GN=cmaA1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 8.4e-06
Identity = 28/75 (37.33%), Postives = 39/75 (52.00%), Query Frame = 0


Query: 37  NISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLK 96
           N+  HYDL S++ F LFLD T  YSCA  +  D  L+ AQ+ KI + + K  +     L 
Sbjct: 11  NVQAHYDL-SDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKLGLQPGMTLL 70

Query: 97  QTGCHWHYFVQTTIE 112
             GC W   +   +E
Sbjct: 71  DVGCGWGATMMRAVE 84

[Close]

BLAST of Carg28083 vs. ExPASy Swiss-Prot
Match: P9WPB6 (Cyclopropane mycolic acid synthase 1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=cmaA1 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 8.4e-06
Identity = 28/75 (37.33%), Postives = 39/75 (52.00%), Query Frame = 0


Query: 37  NISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISVLIEKASINEKHKLK 96
           N+  HYDL S++ F LFLD T  YSCA  +  D  L+ AQ+ KI + + K  +     L 
Sbjct: 11  NVQAHYDL-SDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKLGLQPGMTLL 70

Query: 97  QTGCHWHYFVQTTIE 112
             GC W   +   +E
Sbjct: 71  DVGCGWGATMMRAVE 84

[Close]

BLAST of Carg28083 vs. ExPASy TrEMBL
Match: A0A6J1ICH0 (uncharacterized protein LOC111472470 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472470 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 8.3e-41
Identity = 121/250 (48.40%), Postives = 132/250 (52.80%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HTLRKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI K  DEDLRIAQLRKIS+
Sbjct: 142 QHTLRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISI 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDLGLQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLGLQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 181
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCESVLEDNGLLVLQFISM 321

[Close]

BLAST of Carg28083 vs. ExPASy TrEMBL
Match: A0A6J1IB60 (uncharacterized protein LOC111473008 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473008 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.8e-40
Identity = 120/250 (48.00%), Postives = 132/250 (52.80%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HTLRKNTIAQARKNISRHYDL +NELFSLFLDDTMMYSCAI K  DEDLRIAQLRKIS+
Sbjct: 142 QHTLRKNTIAQARKNISRHYDL-NNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISI 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDLGLQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLGLQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 181
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCESVLEDNGLLVLQFISM 321

[Close]

BLAST of Carg28083 vs. ExPASy TrEMBL
Match: A0A6J1IB85 (LOW QUALITY PROTEIN: uncharacterized protein LOC111473548 OS=Cucurbita maxima OX=3661 GN=LOC111473548 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.8e-40
Identity = 124/265 (46.79%), Postives = 135/265 (50.94%), Query Frame = 0


Query: 6   LLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAII 65
           L    ++ST Y      +HTLRKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI 
Sbjct: 53  LFTATIASTKY----FFQHTLRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIF 112

Query: 66  KLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHW-------------HYFVQTTIEI 125
           K  DEDLRIAQLRKIS+LI+KA IN+KHK+   GC W             H    T  + 
Sbjct: 113 KREDEDLRIAQLRKISILIKKARINKKHKVLDMGCGWGTLAIEIVKQXGCHCTGITLSKQ 172

Query: 126 CPKNAQKRVQDLGLQ--------------------------MIKAVGHEFMGDFFGSYES 181
             K AQKRVQDLGLQ                          MI+AVGHEFM DFFGS ES
Sbjct: 173 QLKYAQKRVQDLGLQDHIEFLLCDYRELSSTTKYDRILSCGMIEAVGHEFMEDFFGSCES 232

[Close]

BLAST of Carg28083 vs. ExPASy TrEMBL
Match: A0A6J1EXS1 (uncharacterized protein LOC111439562 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439562 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 7.8e-39
Identity = 116/245 (47.35%), Postives = 126/245 (51.43%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           +HT RKNTIAQARKNISRHYDL SNELFSLFLDDTMMYSCAI K  DEDLRIAQLRKISV
Sbjct: 142 RHTSRKNTIAQARKNISRHYDL-SNELFSLFLDDTMMYSCAIFKREDEDLRIAQLRKISV 201

Query: 83  LIEKASINEKHK---------------LKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQ 142
           LI+KA IN+KHK               +KQTGCH      +  ++  K AQKRVQDL LQ
Sbjct: 202 LIKKARINKKHKVLDIGCGWGTLAIEIVKQTGCHCTGITLSKQQL--KYAQKRVQDLALQ 261

Query: 143 --------------------------MIKAVGHEFMGDFFGSYESV-------------- 177
                                     MI+AVGHEFM DFFGS ESV              
Sbjct: 262 DHIEFLLCDYRELSSTTKYDRILSCRMIEAVGHEFMEDFFGSCESVLQDNGLLVLQFISV 321

[Close]

BLAST of Carg28083 vs. ExPASy TrEMBL
Match: A0A6J1HLV8 (uncharacterized protein LOC111464165 OS=Cucurbita moschata OX=3662 GN=LOC111464165 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.7e-36
Identity = 108/262 (41.22%), Postives = 132/262 (50.38%), Query Frame = 0


Query: 9   IVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLV 68
           ++ +S  Y  Y   +H+LR+NTI QAR+NISRHYDL SNELFSLFLD TM YSCAI K  
Sbjct: 545 LLTASIAYAKY-FFQHSLRQNTITQARRNISRHYDL-SNELFSLFLDHTMTYSCAIFKRE 604

Query: 69  DEDLRIAQLRKISVLIEKASINEKHKLKQTGCHW-------------HYFVQTTIEICPK 128
           DEDL +AQLRKIS+LI+KA IN+ H + + GC W             HY   T  E   K
Sbjct: 605 DEDLSVAQLRKISILIKKARINKNHHILEIGCGWGSLAIEAVKQTGCHYTGITLSEEQFK 664

Query: 129 NAQKRVQDLGLQ--------------------------MIKAVGHEFMGDFFGSYESV-- 182
            A+K+V+DLGLQ                          MI++VGHEFM DFFGS ESV  
Sbjct: 665 YAEKKVKDLGLQDQIKFLFCDYRELPNNIKYDSIISCGMIESVGHEFMEDFFGSCESVLA 724

[Close]

BLAST of Carg28083 vs. TAIR 10
Match: AT3G23470.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 131.7 bits (330), Expect = 5.9e-31
Identity = 92/249 (36.95%), Postives = 115/249 (46.18%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           KH L++NT+ QARKNIS HYDL SNE F LF+DDTMMYS AI K  +ED R AQ+RKIS+
Sbjct: 153 KHVLKQNTLTQARKNISSHYDL-SNEFFGLFMDDTMMYSSAIFKSENEDPRTAQMRKISL 212

Query: 83  LIEKASINEKHKLKQTGCHWHYF--------------VQTTIEICPKNAQKRVQDLGLQ- 142
           LIEKA I + H++ + GC W  F              +  +IE   K A+ +V++ GLQ 
Sbjct: 213 LIEKARIEKNHEVLEMGCGWGTFAIEVVKRTGCKYTGITLSIEQL-KYAKAKVKEAGLQG 272

Query: 143 -------------------------MIKAVGHEFMGDFFGSYES---------------- 182
                                    MI+AVGHEFM  FF   E                 
Sbjct: 273 RITFMLCDYRQLSDARKYDRIIACEMIEAVGHEFMDKFFSCCEDALAENGIFVLQFTAIP 332

[Close]

BLAST of Carg28083 vs. TAIR 10
Match: AT3G23510.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 91/249 (36.55%), Postives = 118/249 (47.39%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           KH  R+NT+ QAR+NISRHYDL SNELF LFLDDTM YS A+ K  DEDLR AQ+RKIS+
Sbjct: 561 KHVSRQNTLTQARRNISRHYDL-SNELFGLFLDDTMTYSSAVFKSDDEDLRTAQMRKISL 620

Query: 83  LIEKASINEKHKLKQTGCHW--------------HYFVQTTIEICPKNAQKRVQDLGLQ- 142
           LI+KA I + H++ + GC W              +  +  +IE   K A+++V++ GLQ 
Sbjct: 621 LIDKARIEKDHEVLEIGCGWGTLAIEVVRRTGCKYTGITLSIEQL-KYAEEKVKEAGLQD 680

Query: 143 -------------------------MIKAVGHEFMGDFFGSYESV--------------- 182
                                    M++AVGHEFM  FF   E+                
Sbjct: 681 RITFELRDYRQLSDAHKYDRIISCEMLEAVGHEFMEMFFSRCEAALAEDGLMVLQFISTP 740

[Close]

BLAST of Carg28083 vs. TAIR 10
Match: AT3G23530.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 89/249 (35.74%), Postives = 116/249 (46.59%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           KH  R+NT+ QAR+NISRHYDL SNELF  FLDDTM YS A+ K  DEDLR AQ+RKIS+
Sbjct: 561 KHVSRQNTLTQARRNISRHYDL-SNELFGFFLDDTMTYSSAVFKSDDEDLRTAQMRKISL 620

Query: 83  LIEKASINEKHKLKQTGCHW--------------HYFVQTTIEICPKNAQKRVQDLGLQ- 142
           LI+KA I + H++ + GC W              +  +  +IE   K A+++V++ GLQ 
Sbjct: 621 LIDKARIEKDHEVLEIGCGWGTLAIEVVRRTGCKYTGITLSIEQL-KYAEEKVKEAGLQD 680

Query: 143 -------------------------MIKAVGHEFMGDFFGSYESV--------------- 182
                                    M++AVGHEFM  FF   E+                
Sbjct: 681 WITFELRDYRQLSDAQKYDRIISCEMLEAVGHEFMEMFFSRCEAALAENGLIVLQFISIP 740

[Close]

BLAST of Carg28083 vs. TAIR 10
Match: AT3G23480.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 106.3 bits (264), Expect = 2.7e-23
Identity = 85/266 (31.95%), Postives = 116/266 (43.61%), Query Frame = 0


Query: 5   FLLGIVVSSTVYRNYCICKHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAI 64
           FL   + S+T Y      KH  R+NT+ QAR+N+S HYDL SNE F LF+DDTMMYS A+
Sbjct: 139 FLTASLASATYY-----LKHVCRQNTLTQARRNVSSHYDL-SNEFFGLFMDDTMMYSSAV 198

Query: 65  IKLVDEDLRIAQLRKISVLIEKASINEKHKLKQTGCHWH------------YFVQTTIEI 124
            K  +E+LR AQ+RKI +LIEK    E +++ + GC W              +   T+ I
Sbjct: 199 FKSENENLRTAQMRKIHLLIEKDK-EEPYEVLEIGCGWGTLAIEVVKRTGCKYTGFTLSI 258

Query: 125 CP-KNAQKRVQDLGLQ--------------------------MIKAVGHEFMGDFFGSYE 182
              K  +++V++ GLQ                          MI+ VGH+FM  FF   E
Sbjct: 259 EQLKYVEEKVKEAGLQERITFKLCDYRQLCDTQKYDRIISCEMIEHVGHKFMETFFSHCE 318

[Close]

BLAST of Carg28083 vs. TAIR 10
Match: AT3G23460.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-19
Identity = 63/161 (39.13%), Postives = 79/161 (49.07%), Query Frame = 0


Query: 23  KHTLRKNTIAQARKNISRHYDLVSNELFSLFLDDTMMYSCAIIKLVDEDLRIAQLRKISV 82
           KH  R+N + QAR+NISRHYDL SNELF++FLDDTM YS  + K  DE+L+IAQ+RKI +
Sbjct: 139 KHLYRQNNLTQARRNISRHYDL-SNELFTIFLDDTMSYSSGVFKSDDEELKIAQMRKIYL 198

Query: 83  LIEKASINEKHKLKQTGCHWHYFVQTTIEICPKNAQKRVQDLGLQMIKAVGHEFMGDFFG 142
           LIEK                                                        
Sbjct: 199 LIEKT------------------------------------------------------- 238

Query: 143 SYESVSRKMVFVEHLENIGIHYYRTLRCLDKEFLEQSKQNT 184
           +Y S S     +E++ENIGIHYY+TLR   K F E+ KQ T
Sbjct: 259 AYLSCS-----IENVENIGIHYYQTLRLWRKNFFERQKQIT 238

The following BLAST results are available for this feature:

BLAST of Carg28083 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018231.1	2.4e-98	100.00	ufaA1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022973880.1	1.7e-40	48.40	uncharacterized protein LOC111472470 isoform X2 [Cucurbita maxima]	[more]
XP_022974371.1	3.8e-40	48.00	uncharacterized protein LOC111473008 isoform X1 [Cucurbita maxima]	[more]
XP_022974792.1	3.8e-40	46.79	LOW QUALITY PROTEIN: uncharacterized protein LOC111473548 [Cucurbita maxima]	[more]
XP_022932946.1	1.6e-38	47.35	uncharacterized protein LOC111439562 isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg28083 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O53732	4.4e-07	33.55	Tuberculostearic acid methyltransferase UfaA1 OS=Mycobacterium tuberculosis (str...	[more]
P9WPB2	4.9e-06	38.67	Cyclopropane mycolic acid synthase 3 OS=Mycobacterium tuberculosis (strain CDC 1...	[more]
P9WPB3	4.9e-06	38.67	Cyclopropane mycolic acid synthase 3 OS=Mycobacterium tuberculosis (strain ATCC ...	[more]
A5U866	8.4e-06	37.33	Cyclopropane mycolic acid synthase 1 OS=Mycobacterium tuberculosis (strain ATCC ...	[more]
P9WPB6	8.4e-06	37.33	Cyclopropane mycolic acid synthase 1 OS=Mycobacterium tuberculosis (strain CDC 1...	[more]

BLAST of Carg28083 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ICH0	8.3e-41	48.40	uncharacterized protein LOC111472470 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IB60	1.8e-40	48.00	uncharacterized protein LOC111473008 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IB85	1.8e-40	46.79	LOW QUALITY PROTEIN: uncharacterized protein LOC111473548 OS=Cucurbita maxima OX...	[more]
A0A6J1EXS1	7.8e-39	47.35	uncharacterized protein LOC111439562 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1HLV8	4.7e-36	41.22	uncharacterized protein LOC111464165 OS=Cucurbita moschata OX=3662 GN=LOC1114641...	[more]

BLAST of Carg28083 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23470.1	5.9e-31	36.95	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23510.1	1.0e-30	36.55	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23530.1	1.5e-29	35.74	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23480.1	2.7e-23	31.95	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23460.1	1.4e-19	39.13	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02353	CMAS	coord: 33..111 e-value: 3.6E-17 score: 62.5
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 21..125 e-value: 8.2E-17 score: 63.2
None	No IPR available	PANTHER	PTHR43675:SF22	SUBFAMILY NOT NAMED	coord: 152..179
None	No IPR available	PANTHER	PTHR43675:SF22	SUBFAMILY NOT NAMED	coord: 20..107
IPR026669	Arsenite methyltransferase-like	PANTHER	PTHR43675	ARSENITE METHYLTRANSFERASE	coord: 20..107
IPR026669	Arsenite methyltransferase-like	PANTHER	PTHR43675	ARSENITE METHYLTRANSFERASE	coord: 152..179
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 21..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg28083-RA	Carg28083-RA	mRNA