Carg28005 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg28005
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr14: 14220904 .. 14221728 (+)
RNA-Seq ExpressionCarg28005
SyntenyCarg28005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCGATTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATTGAAAAATGGCTTTTCACTTACAACAAAAGAACATGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTTATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCGATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATCGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATGGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTGTTTTTTACGCCGTGGAAAATCCTCAAAGAAATATTTGCCAAAAACACCAATTACAATTGGGGTTTTGGTTTAAATGGAAAGAACAGGGTCTAGATTTGATTCTCTTCAAGCCCATTGTTGGAGGCATTGTAATAGAACGAATTTTCGAACAAAAGTCAAGGCTCGTGTGCCAATCTGGGATGTATGAATAA

mRNA sequence

ATGGAGCGATTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATTGAAAAATGGCTTTTCACTTACAACAAAAGAACATGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTTATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCGATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATCGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATGGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTGTTTTTTACGCCGTGGAAAATCCTCAAAGAAATATTTGCCAAAAACACCAATTACAATTGGGGTTTTGGTTTAAATGGAAAGAACAGGGTCTAGATTTGATTCTCTTCAAGCCCATTGTTGGAGGCATTGTAATAGAACGAATTTTCGAACAAAAGTCAAGGCTCGTGTGCCAATCTGGGATGTATGAATAA

Coding sequence (CDS)

ATGGAGCGATTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATTGAAAAATGGCTTTTCACTTACAACAAAAGAACATGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTTATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCGATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATCGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATGGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTGTTTTTTACGCCGTGGAAAATCCTCAAAGAAATATTTGCCAAAAACACCAATTACAATTGGGGTTTTGGTTTAAATGGAAAGAACAGGGTCTAGATTTGATTCTCTTCAAGCCCATTGTTGGAGGCATTGTAATAGAACGAATTTTCGAACAAAAGTCAAGGCTCGTGTGCCAATCTGGGATGTATGAATAA

Protein sequence

MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQGLDLILFKPIVGGIVIERIFEQKSRLVCQSGMYE
Homology
BLAST of Carg28005 vs. NCBI nr
Match: KAG7018988.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 544.3 bits (1401), Expect = 6.1e-151
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240

Query: 241 GLDLILFKPIVGGIVIERIFEQKSRLVCQSGMYE 275
           GLDLILFKPIVGGIVIERIFEQKSRLVCQSGMYE
Sbjct: 241 GLDLILFKPIVGGIVIERIFEQKSRLVCQSGMYE 274

BLAST of Carg28005 vs. NCBI nr
Match: XP_022924647.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 426.8 bits (1096), Expect = 1.4e-115
Identity = 228/251 (90.84%), Postives = 234/251 (93.23%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. NCBI nr
Match: KAG7018915.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424.5 bits (1090), Expect = 7.1e-115
Identity = 226/251 (90.04%), Postives = 234/251 (93.23%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSM+EMRDEVKSEFIRLNGIGIVVNVV+QILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMMEMRDEVKSEFIRLNGIGIVVNVVLQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. NCBI nr
Match: XP_023528267.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.8 bits (1083), Expect = 4.6e-114
Identity = 227/251 (90.44%), Postives = 233/251 (92.83%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NEDKLFDEMSKREE MKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDKLFDEMSKREELMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. NCBI nr
Match: XP_022980298.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 420.2 bits (1079), Expect = 1.3e-113
Identity = 226/251 (90.04%), Postives = 233/251 (92.83%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NED+LFDEMSKREE MKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.7e-18
Identity = 67/210 (31.90%), Postives = 111/210 (52.86%), Query Frame = 0

Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLA-TVESSPFKVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 136 MIEMRDEVKSEFIRLNGI-GIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKL 195
           ++   +      +   G+   + N+V       +D       +EAL +L     S E  L
Sbjct: 131 IVS-ENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVL 190

Query: 196 FDEMSKREE--WMKSVAIVLQRGSAEGRVF 214
            + ++ +++   +KS+  ++QRG  E RV+
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVY 208

BLAST of Carg28005 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.6e-16
Identity = 48/122 (39.34%), Postives = 75/122 (61.48%), Query Frame = 0

Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+  K +CP T Q +   E D  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETD--L 65

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLATVESSPF-KVSSLRKLRS 129
           TPN TL+R+I +W  +N S       +  P     +++ L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 123

BLAST of Carg28005 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.6e-16
Identity = 64/210 (30.48%), Postives = 100/210 (47.62%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   N  TCP T   +    +DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 78  NLTLKRVILAWKINESS---------------SSPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S               +S    + Q  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 138 KLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNE 197
           +LR      D+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 198 DKLFDEMSKREEWMKSVAIVLQRGSAEGRV 213
              F  +S     ++ +  +L   S E RV
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRV 213

BLAST of Carg28005 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-13
Identity = 68/208 (32.69%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  F+CPIS+++M+ PV++STGVTY+R SI++WL   N  TCPATMQ +     +FV  P
Sbjct: 12  PCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQ--NKEFV--P 71

Query: 78  NLTLKRVILAW--KINE--SSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDE 137
           NLTL R+I  W   IN    S SP +       + A +E    +  +  K+       DE
Sbjct: 72  NLTLHRLIDHWSDSINRRADSESPESDTPTRDEINAAIERFRIENDARSKILRFARESDE 131

Query: 138 VKSEFIRLNGIGIVVNVVVQILIDCSDF--STLEACEEALGVLSQFPISNEDKLFDE--- 197
              EF  L G    V ++V ++ D  +F  S L    EA+ +LS        K+FD    
Sbjct: 132 -NREF--LAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMI----RRKIFDRRRL 191

Query: 198 ----MSKREEWMKSVAIVLQRGSAEGRV 213
               ++   + + S  ++++RG+ + ++
Sbjct: 192 SNLILTNGGDCLTSFFLLIKRGNPKLKI 207

BLAST of Carg28005 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 8.1e-13
Identity = 49/137 (35.77%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS++LM DPVTISTG TY+R SI+ W+   N  TCP T     V  +DF + P
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVT----RVALSDFTLIP 74

Query: 78  NLTLKRVILAWKINESSS---------------SPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S+               S  + + Q  ++  T  S   + +++R
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 134

Query: 138 KLRSMIEMRDEVKSEFI 140
           +LR +   RD  K+  +
Sbjct: 135 RLRGL--ARDSEKNRVL 144

BLAST of Carg28005 vs. ExPASy TrEMBL
Match: A0A6J1E9J6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.9e-116
Identity = 228/251 (90.84%), Postives = 234/251 (93.23%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 6.5e-114
Identity = 226/251 (90.04%), Postives = 233/251 (92.83%), Query Frame = 0

Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQ 240
           NED+LFDEMSKREE MKSVAIVLQRGSAEGR FYAV+   RNI +      G  F  +EQ
Sbjct: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--FVMEEQ 240

Query: 241 GLDLILFKPIV 252
           G+D  LFKP++
Sbjct: 241 GID--LFKPLL 245

BLAST of Carg28005 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 7.4e-94
Identity = 184/239 (76.99%), Postives = 209/239 (87.45%), Query Frame = 0

Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRSMIEM D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNEDKLFDEM KR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQGLDLILFKPIV 252
           EE MKS+ +VLQRGSAEGR FYAV+   RNI +      G     +EQ +D   FKP++
Sbjct: 181 EELMKSITVVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--SVMEEQAID--FFKPLL 233

BLAST of Carg28005 vs. ExPASy TrEMBL
Match: A0A1S3AWJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 2.2e-93
Identity = 183/239 (76.57%), Postives = 209/239 (87.45%), Query Frame = 0

Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRS+IEM D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNEDKLFDEM KR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQGLDLILFKPIV 252
           EE MKS+ +VLQRGSAEGR FYAV+   RNI +      G     +EQ +D   FKP++
Sbjct: 181 EELMKSITVVLQRGSAEGR-FYAVK-ILRNIAKNTNYNWG--SVMEEQAID--FFKPLL 233

BLAST of Carg28005 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 6.3e-93
Identity = 182/239 (76.15%), Postives = 208/239 (87.03%), Query Frame = 0

Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G  D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNL LKR+ILAWKIN+S+ +PATA+++V SLLATVESSPFKVSSLRKLRSM+EM D
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192
            +KSEFIRL+GIGIVVNVV+QILIDCSDFSTL+ CEEALGVL QFPISNEDKLFDEM KR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EEWMKSVAIVLQRGSAEGRVFYAVENPQRNICQKHQLQLGFWFKWKEQGLDLILFKPIV 252
           EE MKS+A+V+QRGSAEGR FYAV+   RNI +      G     +EQG+D   FKP++
Sbjct: 181 EELMKSIAVVVQRGSAEGR-FYAVK-ILRNIAKNTNYNWGSIM--EEQGID--FFKPLL 233

BLAST of Carg28005 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 94.7 bits (234), Expect = 1.2e-19
Identity = 67/210 (31.90%), Postives = 111/210 (52.86%), Query Frame = 0

Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLA-TVESSPFKVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 136 MIEMRDEVKSEFIRLNGI-GIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKL 195
           ++   +      +   G+   + N+V       +D       +EAL +L     S E  L
Sbjct: 131 IVS-ENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVL 190

Query: 196 FDEMSKREE--WMKSVAIVLQRGSAEGRVF 214
            + ++ +++   +KS+  ++QRG  E RV+
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVY 208

BLAST of Carg28005 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 86.7 bits (213), Expect = 3.3e-17
Identity = 48/122 (39.34%), Postives = 75/122 (61.48%), Query Frame = 0

Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+  K +CP T Q +   E D  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETD--L 65

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLATVESSPF-KVSSLRKLRS 129
           TPN TL+R+I +W  +N S       +  P     +++ L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 123

BLAST of Carg28005 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 86.7 bits (213), Expect = 3.3e-17
Identity = 64/210 (30.48%), Postives = 100/210 (47.62%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   N  TCP T   +    +DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 78  NLTLKRVILAWKINESS---------------SSPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S               +S    + Q  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 138 KLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNE 197
           +LR      D+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 198 DKLFDEMSKREEWMKSVAIVLQRGSAEGRV 213
              F  +S     ++ +  +L   S E RV
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRV 213

BLAST of Carg28005 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-14
Identity = 68/208 (32.69%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  F+CPIS+++M+ PV++STGVTY+R SI++WL   N  TCPATMQ +     +FV  P
Sbjct: 12  PCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQ--NKEFV--P 71

Query: 78  NLTLKRVILAW--KINE--SSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDE 137
           NLTL R+I  W   IN    S SP +       + A +E    +  +  K+       DE
Sbjct: 72  NLTLHRLIDHWSDSINRRADSESPESDTPTRDEINAAIERFRIENDARSKILRFARESDE 131

Query: 138 VKSEFIRLNGIGIVVNVVVQILIDCSDF--STLEACEEALGVLSQFPISNEDKLFDE--- 197
              EF  L G    V ++V ++ D  +F  S L    EA+ +LS        K+FD    
Sbjct: 132 -NREF--LAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMI----RRKIFDRRRL 191

Query: 198 ----MSKREEWMKSVAIVLQRGSAEGRV 213
               ++   + + S  ++++RG+ + ++
Sbjct: 192 SNLILTNGGDCLTSFFLLIKRGNPKLKI 207

BLAST of Carg28005 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 75.9 bits (185), Expect = 5.7e-14
Identity = 49/137 (35.77%), Postives = 72/137 (52.55%), Query Frame = 0

Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS++LM DPVTISTG TY+R SI+ W+   N  TCP T     V  +DF + P
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVT----RVALSDFTLIP 74

Query: 78  NLTLKRVILAWKINESSS---------------SPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S+               S  + + Q  ++  T  S   + +++R
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 134

Query: 138 KLRSMIEMRDEVKSEFI 140
           +LR +   RD  K+  +
Sbjct: 135 RLRGL--ARDSEKNRVL 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018988.16.1e-151100.00E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022924647.11.4e-11590.84E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata][more]
KAG7018915.17.1e-11590.04E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023528267.14.6e-11490.44E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
XP_022980298.11.3e-11390.04E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84TG31.7e-1831.90E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC64.6e-1639.34E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9LT794.6e-1630.48U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9LXE33.6e-1332.69U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Q9FXA48.1e-1335.77U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1E9J66.9e-11690.84RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079... [more]
A0A6J1IYW36.5e-11490.04RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P... [more]
A0A5D3D2G27.4e-9476.99RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AWJ22.2e-9376.57RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 ... [more]
A0A0A0L6J26.3e-9376.15RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE... [more]
Match NameE-valueIdentityDescription
AT2G35930.11.2e-1931.90plant U-box 23 [more]
AT3G52450.13.3e-1739.34plant U-box 22 [more]
AT3G19380.13.3e-1730.48plant U-box 25 [more]
AT5G09800.12.6e-1432.69ARM repeat superfamily protein [more]
AT1G49780.15.7e-1435.77plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 20..88
e-value: 1.9E-17
score: 74.0
IPR003613U box domainPFAMPF04564U-boxcoord: 18..90
e-value: 5.5E-15
score: 55.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 16..95
score: 18.736959
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 3..106
e-value: 3.1E-22
score: 80.3
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 17..250
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 17..250
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 21..64
e-value: 8.25594E-22
score: 83.8222
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 11..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28005-RACarg28005-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity