Carg28004 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg28004
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionent-kaurenoic acid oxidase 2-like
LocationCarg_Chr14: 14216481 .. 14217176 (+)
RNA-Seq ExpressionCarg28004
SyntenyCarg28004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATTACTACCTGGATTTGCTTTAGCGCAACAAAGCACTCAAGGGTATTGATAATCATGCCTCAATACATTTCGAAATGTAAGTTTGGATTCTGGAAATTTGTTCGATCAAGTGTAAAGCCACCGATTACTTGTTTAGGCTCGGAAGATCTTGTGGCTGCGCTGAAAGAATTGTGACTGAGCGAGGACGCGCAAAATTCGAACCAAATGGGCGCCGAAGAGGAAGGACATGATGGACCAACCTGATCGATATTGAAAGATTAAAATGGCCAGGAAACTTGGACGACGAGGAGATCAAGTCGATCTCTGATAATGTAACCTTGAACTGCAGGCTCATTGATCTTCAGCCACACACCATGATGTGGCGAACTATTTTTTGGTTGGATCATACACGCCTGAAGTTCTCAAAAAAGCCAGGTATCGTACACAATTAGGAACTGAGATTAGCCTGTTTGGTTACTCAAAGAATCATGCATATTTTCTCATATAGACAATCCCTGAAGCAATTTGATTAAAAATGATACAGGAAAGAACAGGGAAGCGATTGTGAGGAACAGGCCTGCAGGTCAAACGGGATTAACATCGAAAGAAGCGGGACATGGAATATCTCTCAAGTTGTTGACGAGACTCTTCGCTATGTATATCTCCCTGTTGTTTTCTCAGAGAGGCCACAAATGGATGTCAACCTCAAAT

mRNA sequence

ATGAGAATTACTACCTGGATTTGCTTTAGCGCAACAAAGCACTCAAGGGTATTGATAATCATGCCTCAATACATTTCGAAATGTAAGTTTGGATTCTGGAAATTTGTTCGATCAAGTGTAAAGCCACCGATTACTTGTTTAGGCTCGGAAGATCTTGTGGCTGCGCTGAAAGAATTATTAAAATGGCCAGGAAACTTGGACGACGAGGAGATCAAGTCGATCTCTGATAATGTAACCTTGAACTGCAGGCTCATTGATCTTCAGCCACACACCATGATGTGGCGAACTATTTTTTGGTTGGATCATACACGCCTGAAGTTCTCAAAAAAGCCAGGAAAGAACAGGGAAGCGATTGTGAGGAACAGGCCTGCAGGTCAAACGGGATTAACATCGAAAGAAGCGGGACATGGAATATCTCTCAAGTTGTTGACGAGACTCTTCGCTATGTATATCTCCCTGTTGTTTTCTCAGAGAGGCCACAAATGGATGTCAACCTCAAAT

Coding sequence (CDS)

ATGAGAATTACTACCTGGATTTGCTTTAGCGCAACAAAGCACTCAAGGGTATTGATAATCATGCCTCAATACATTTCGAAATGTAAGTTTGGATTCTGGAAATTTGTTCGATCAAGTGTAAAGCCACCGATTACTTGTTTAGGCTCGGAAGATCTTGTGGCTGCGCTGAAAGAATTATTAAAATGGCCAGGAAACTTGGACGACGAGGAGATCAAGTCGATCTCTGATAATGTAACCTTGAACTGCAGGCTCATTGATCTTCAGCCACACACCATGATGTGGCGAACTATTTTTTGGTTGGATCATACACGCCTGAAGTTCTCAAAAAAGCCAGGAAAGAACAGGGAAGCGATTGTGAGGAACAGGCCTGCAGGTCAAACGGGATTAACATCGAAAGAAGCGGGACATGGAATATCTCTCAAGTTGTTGACGAGACTCTTCGCTATGTATATCTCCCTGTTGTTTTCTCAGAGAGGCCACAAATGGATGTCAACCTCAAAT

Protein sequence

MRITTWICFSATKHSRVLIIMPQYISKCKFGFWKFVRSSVKPPITCLGSEDLVAALKELLKWPGNLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFSKKPGKNREAIVRNRPAGQTGLTSKEAGHGISLKLLTRLFAMYISLLFSQRGHKWMSTSN
Homology
[Close]
BLAST of Carg28004 vs. NCBI nr
Match: KAG7018987.1 (hypothetical protein SDJN02_20864, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348.2 bits (892), Expect = 3.9e-92
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0 

Query: 1   MRITTWICFSATKHSRVLIIMPQYISKCKFGFWKFVRSSVKPPITCLGSEDLVAALKELL 60
           MRITTWICFSATKHSRVLIIMPQYISKCKFGFWKFVRSSVKPPITCLGSEDLVAALKELL
Sbjct: 1   MRITTWICFSATKHSRVLIIMPQYISKCKFGFWKFVRSSVKPPITCLGSEDLVAALKELL 60

Query: 61  KWPGNLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFSKKPGKNREAIVR 120
           KWPGNLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFSKKPGKNREAIVR
Sbjct: 61  KWPGNLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFSKKPGKNREAIVR 120

Query: 121 NRPAGQTGLTSKEAGHGISLKLLTRLFAMYISLLFSQRGHKWMSTSN 168
           NRPAGQTGLTSKEAGHGISLKLLTRLFAMYISLLFSQRGHKWMSTSN
Sbjct: 121 NRPAGQTGLTSKEAGHGISLKLLTRLFAMYISLLFSQRGHKWMSTSN 167

[Close]
BLAST of Carg28004 vs. NCBI nr
Match: XP_023528639.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo] >KAG6582529.1 Ent-kaurenoic acid oxidase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018914.1 Ent-kaurenoic acid oxidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-04
Identity = 32/83 (38.55%), Postives = 47/83 (56.63%), Query Frame = 0 

Query: 57  KELLKWPGNLDDEEIKSISDNVTLNCRLIDLQP------HTMMWRTIFWLDHTRLKFSKK 116
           K+++    +++DE  + + D   ++  ++ L        HTMMW TI    H  +   KK
Sbjct: 270 KDMMDQLIDIEDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQHPEV--LKK 329

Query: 117 PGKNREAIVRNRPAGQTGLTSKE 134
             + +EAIVRNRPAGQTGLT KE
Sbjct: 330 ATEEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. NCBI nr
Match: XP_022924644.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita moschata])

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-04
Identity = 32/83 (38.55%), Postives = 47/83 (56.63%), Query Frame = 0 

Query: 57  KELLKWPGNLDDEEIKSISDNVTLNCRLIDLQP------HTMMWRTIFWLDHTRLKFSKK 116
           K+++    +++DE  + + D   ++  ++ L        HTMMW TI    H  +   KK
Sbjct: 270 KDMMDQLIDIEDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQHPEV--LKK 329

Query: 117 PGKNREAIVRNRPAGQTGLTSKE 134
             + +EAIVRNRPAGQTGLT KE
Sbjct: 330 AREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. NCBI nr
Match: XP_022980275.1 (ent-kaurenoic acid oxidase 2-like [Cucurbita maxima])

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-04
Identity = 39/85 (45.88%), Postives = 50/85 (58.82%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPG-NLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFS 109
           +D++  L +++   G  LDDEEI  I   + LN    +   HTMMW TI    H  +   
Sbjct: 270 KDMMDQLIDIVDENGRKLDDEEIIDIL-IMYLNAG-HESSGHTMMWATILLNQHPEV--L 329

Query: 110 KKPGKNREAIVRNRPAGQTGLTSKE 134
           KK  + +EAIVRNRPAGQTGLT KE
Sbjct: 330 KKAREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. NCBI nr
Match: AAG41777.1 (ent-kaurenoic acid oxidase [Cucurbita maxima])

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-04
Identity = 39/85 (45.88%), Postives = 50/85 (58.82%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPG-NLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFS 109
           +D++  L +++   G  LDDEEI  I   + LN    +   HTMMW TI    H  +   
Sbjct: 270 KDMMDQLIDIVDENGRKLDDEEIIDIL-IMYLNAG-HESSGHTMMWATILLNQHPEV--L 329

Query: 110 KKPGKNREAIVRNRPAGQTGLTSKE 134
           KK  + +EAIVRNRPAGQTGLT KE
Sbjct: 330 KKAREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.5e-04
Identity = 26/90 (28.89%), Postives = 51/90 (56.67%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPGNLDDEEIKSISDNVTLNCRLIDLQP------HTMMWRTIFWLDHT 109
           +++++  K++L    N+ DE+ K++ D   ++  L+ L        HT+MW T+F  +H 
Sbjct: 261 QNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHP 320

Query: 110 RLKFSKKPGKNREAIVRNRPAGQTGLTSKE 134
            +   ++    +E I+++RP GQ GL+ KE
Sbjct: 321 EV--LQRAKAEQEMILKSRPEGQKGLSLKE 348

[Close]
BLAST of Carg28004 vs. ExPASy TrEMBL
Match: A0A6J1IVT9 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.3e-04
Identity = 39/85 (45.88%), Postives = 50/85 (58.82%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPG-NLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFS 109
           +D++  L +++   G  LDDEEI  I   + LN    +   HTMMW TI    H  +   
Sbjct: 270 KDMMDQLIDIVDENGRKLDDEEIIDIL-IMYLNAG-HESSGHTMMWATILLNQHPEV--L 329

Query: 110 KKPGKNREAIVRNRPAGQTGLTSKE 134
           KK  + +EAIVRNRPAGQTGLT KE
Sbjct: 330 KKAREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. ExPASy TrEMBL
Match: Q9FQY4 (Ent-kaurenoic acid oxidase OS=Cucurbita maxima OX=3661 GN=CYP88A2 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.3e-04
Identity = 39/85 (45.88%), Postives = 50/85 (58.82%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPG-NLDDEEIKSISDNVTLNCRLIDLQPHTMMWRTIFWLDHTRLKFS 109
           +D++  L +++   G  LDDEEI  I   + LN    +   HTMMW TI    H  +   
Sbjct: 270 KDMMDQLIDIVDENGRKLDDEEIIDIL-IMYLNAG-HESSGHTMMWATILLNQHPEV--L 329

Query: 110 KKPGKNREAIVRNRPAGQTGLTSKE 134
           KK  + +EAIVRNRPAGQTGLT KE
Sbjct: 330 KKAREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. ExPASy TrEMBL
Match: A0A6J1E9S4 (ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.3e-04
Identity = 32/83 (38.55%), Postives = 47/83 (56.63%), Query Frame = 0 

Query: 57  KELLKWPGNLDDEEIKSISDNVTLNCRLIDLQP------HTMMWRTIFWLDHTRLKFSKK 116
           K+++    +++DE  + + D   ++  ++ L        HTMMW TI    H  +   KK
Sbjct: 270 KDMMDQLIDIEDENGRKLDDEEIIDILIMYLNAGHESSGHTMMWATILLNQHPEV--LKK 329

Query: 117 PGKNREAIVRNRPAGQTGLTSKE 134
             + +EAIVRNRPAGQTGLT KE
Sbjct: 330 AREEQEAIVRNRPAGQTGLTLKE 350

[Close]
BLAST of Carg28004 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 45.8 bits (107), Expect = 3.9e-05
Identity = 26/90 (28.89%), Postives = 51/90 (56.67%), Query Frame = 0 

Query: 50  EDLVAALKELLKWPGNLDDEEIKSISDNVTLNCRLIDLQP------HTMMWRTIFWLDHT 109
           +++++  K++L    N+ DE+ K++ D   ++  L+ L        HT+MW T+F  +H 
Sbjct: 261 QNILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHP 320

Query: 110 RLKFSKKPGKNREAIVRNRPAGQTGLTSKE 134
            +   ++    +E I+++RP GQ GL+ KE
Sbjct: 321 EV--LQRAKAEQEMILKSRPEGQKGLSLKE 348

The following BLAST results are available for this feature:
BLAST of Carg28004 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7018987.13.9e-92100.00hypothetical protein SDJN02_20864, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023528639.19.0e-0438.55ent-kaurenoic acid oxidase 2-like [Cucurbita pepo subsp. pepo] >KAG6582529.1 Ent... [more]
XP_022924644.19.0e-0438.55ent-kaurenoic acid oxidase 2-like [Cucurbita moschata][more]
XP_022980275.19.0e-0445.88ent-kaurenoic acid oxidase 2-like [Cucurbita maxima][more]
AAG41777.19.0e-0445.88ent-kaurenoic acid oxidase [Cucurbita maxima][more]
BLAST of Carg28004 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O230515.5e-0428.89Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
BLAST of Carg28004 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1IVT94.3e-0445.88ent-kaurenoic acid oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479695 PE... [more]
Q9FQY44.3e-0445.88Ent-kaurenoic acid oxidase OS=Cucurbita maxima OX=3661 GN=CYP88A2 PE=2 SV=1[more]
A0A6J1E9S44.3e-0438.55ent-kaurenoic acid oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432076 ... [more]
BLAST of Carg28004 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G05160.13.9e-0528.89cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28004-RACarg28004-RAmRNA