Carg27887 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27887
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDead box ATP-dependent RNA helicase, putative
LocationCarg_Chr11: 7823860 .. 7827186 (+)
RNA-Seq ExpressionCarg27887
SyntenyCarg27887
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAGAGTTTTGTGTTCGATCCCCCCAGTGACGAGGAATTCGACCACTCCGATGCTGAAGAAGAAGAAGAAGATGAAGAACAAGAAGAACGAGAAGAGGAACAAGGAGGAAAAGAGGAGGAAGATGAACCCCTTTCTCGTCGTCGCACTGAGTCTCCTTGGGATTTTGCCTCTTACTCCGAGTCGGTGGCTGAAGAACATGCCCGTAGAAGCACTACTTCTGTTGATTTCAAAATTTCCAAACTGCTTGAGAAGCGCTCCTCACACGTCACTCCTACCGCGGCTGATGATGATGAGAGTTCGGAAGAGGAATCTGACAGACAAGTAACTTTGTGGACCTATCTCTATTGCGCTATTTGGTTGTTTTATGACATTGCTAATTCTGCCGCTTTGATTTTTAGGAAGATTATAGACCGGAAGACGACGATGATGGAACTAGTAATGCTGGTGAGGGTAAGTCGTTTTTTGCGCCATCCGATGGGGCTTCCTTTCATGCTAATTCGTTCATGGAGCTTAACTTGTCGCGCCCACTGATTCGAGCATGTGAGGCTTTGGGATATGCAAAGCCGACGCCAATTCAGGTTCTTTTTCTATGATTTTTGTTTTTATTTTATTTATTTTTATATTCTTGAATTTTATGTTCTTATTAATCTTGTTGGGTAGGCGGCTTGCATACCGTTGGCATTGACTGGACGTGATATATGCGGAAGTGCAATTACTGGTTCTGGAAAGGTATGGGTTTATCTTGAGCTTTCTTTGTAGTTAATTGTTACGAGAATTAGAGTTGGAATAGGATGGTAGTTTTTTCGGGGAATTTGTTTTATGAACTATTGCAAAAATTCTATAAGCTGGAAAACTACAGAATGATTTGGAAAAGATGTGTGAAAGCCCTAGTATTGCTTTTAATCTTACCTTGTTCTCACAGACTGCAGCTTTTTCGCTACCTACTTTGGAGAGGTTACTCTATCGACCCAAACGTGATCGTGCAATAAGAGTTTTAATTCTAACTCCAGCTAGAGAATTGGCAATCCAGTAAGTATGTTTTTAAAGATACGTATTTTTTATCATGTGTTTTCATATAATCAACCGATTTAGATGTCAGTTAAAATTCAGTCTACATATATATCTTAATGGAAAGTTGTTGGTTAGCTTTTTAATGAAGTGCCCAGTTTTAGTTGTTGCACGTCCATCACCTTGATAAAACTTGGTCTCATTTTTCTTGCAAAATGCAAATTTCATGCTGTCATTTATGGTTTCCATAATAAATGTCAACTGAAAAACTATATTTGGATATTGATATAAATATTTGGTGGTATTCTCTGAGAATTCTGTTCTAGGCTACATTGGGTATGCGGCCATCTTTTAAAATACAAGACAAATATCTTGTGGCTCAATGCTTGCTTAGGCTCTATATCACTTGAAAGATATTGAAGAAGCTTTTAAGGGCTAGATTCTGATTGGTTAAGAAGCTTTAAAGGGTTAGAATGAATTCATGGAAGTCTCTAGAGTAATGGAGAAAGGTTTGGATGCAGGATAAAATAAAACTGATTTCTGGAGGTTGTGGAAAAAGAAAAGAGAGGAAATGCCATAGGAAGTAAGAAGAGACAAAGATGAGAGAGAGAATAATATTAAGAAGTGTGAAATAGTGTGGTGTATCAAGATAAGTTGGAAAAAAATATTAGAGGAAAAGGCAAAAGAAAGAGTGGTTTGAAGGAAAAAAACAAAGTAGGAGGTAAAGAAAAGGGAGAAAAAGAGAAAGGAGCCTGTTGTTGAAAAAGAAGAAGAAAAGTTGGTGGAAAAATGAGTGTGTGTGTGTGGAAAAGATGGAAAGAGTGAAAAATATAGAGGTTGTAATCTCTCAGACTCACGACATCCACCTGTTTTGCGTACAAGGTGTTTCCATATTTTTACCATGTTTCTTGGTTTTTCTTTTCAATCTCAATTGTAGTGTTGGAGTCTTTTTGGAAGAGATTATTTAAATGTGGGAGATAAGAAAGAATCAGTGAACTAAAAAGAATAGCAATTGAAAAAGGAGGGGAAAAAATCCCTTAAATCAACTTTTTGTCTCCCTACGCTTGAGTGAAGCAATCAGATTTGTTGCATTGAGGTGCTACCACTTTTAAAAAACTCTTTTTTTAGTTCAGGGCTTTTGTTGGTCATTGGAGTGGAGATTTGTGATTCAAGCGACATTATTATTGTTTTCCTTTTTCACAAACAATGTTACAAAAAACTCCTGGTTTGGAGAATATTAAAGCAGCCCTAAAGAACCTTCCAAAAGTTAATAAAATCTCTCTCTCGTCCCCCCCCCCCCCCCCCCCCCCAAAACTGAAAAGACAAACTGAAAAAGTATCACCTAAATTTTGAAAACTATATAAACCCGCTGAGGACATGCACATTGTAACACCACATTATGAACCTCTGACCACCCTTTGGAGAAGCTAAAGAAAGGTAAGTCTAATATGAAGCCCTAAAAGAACTCCAATCGGAAGCATGTGCCTCACTAACTTCATGATAAGCCTCAATCAATAAGAAACATGGTTGTTGAAGAGATTCTACAACCATATTTTTTATACCTAAGAGCATGACCCACTTCTCCAAGGGCAGAAGAGTTGCCATATTAATCGTCTTTGACCCACCTAATGTCTCCTTATATATAAAAGCTATATATTTCATTTAGTGCCAAATTAGACACAAAACAAAAATTGAGCATGCTTTGAAAGATCTATTTTGGTACACGTATTCCTCTTGTCCTCTACATTTTCATGAATTATAATACTAACTGAAAATAAGGAAGTTTTGCTGTTTTTTCCTCCAGAGTTCACAGTATGAATAGAGAAACTTGCTCAGTTTACTGATATCAGATGTTTGCTGACAATCGTTGGACCTTTCCTGGGCTTTCAGAAAAGGTATGAGCACCTAGTGTACATTTTGTGACTAAAGAAGAAGTTTGAAAAATTTCTACATATAGTAAACATTTTACTGGGATGTCTAATTCTATATTGTAGAATTATTTCTTTTAAAAAATTCGTTATGTATTCCTTTACATGTATTCACGGTCCTGTTTCATTCATAAGATGTGAGGTTTTATTGAAGATCAAAATGCATTACTAACAGGGGTTTTGACTCGTGTTTCACGAAATATGGTTGAATTTGCTCTCCTTTCTTTGGTTAAGGATATTTTATTTTCTTAATTTTGTTCAGGAAGCAAGAGGCTGCCTTAAGATCGATCCTGATGTGGTTGTGGCCACTCCAGGACGCATGATCGATCATCTACGCAATTCCATGTCCGTAGACTTGGACGATTTAGCAAGTTTTAATACTTGA

mRNA sequence

ATGGCTAAGAGTTTTGTGTTCGATCCCCCCAGTGACGAGGAATTCGACCACTCCGATGCTGAAGAAGAAGAAGAAGATGAAGAACAAGAAGAACGAGAAGAGGAACAAGGAGGAAAAGAGGAGGAAGATGAACCCCTTTCTCGTCGTCGCACTGAGTCTCCTTGGGATTTTGCCTCTTACTCCGAGTCGGTGGCTGAAGAACATGCCCGTAGAAGCACTACTTCTGTTGATTTCAAAATTTCCAAACTGCTTGAGAAGCGCTCCTCACACGTCACTCCTACCGCGGCTGATGATGATGAGAGTTCGGAAGAGGAATCTGACAGACAAGAAGATTATAGACCGGAAGACGACGATGATGGAACTAGTAATGCTGGTGAGGGTAAGTCGTTTTTTGCGCCATCCGATGGGGCTTCCTTTCATGCTAATTCGTTCATGGAGCTTAACTTGTCGCGCCCACTGATTCGAGCATGTGAGGCTTTGGGATATGCAAAGCCGACGCCAATTCAGGCGGCTTGCATACCGTTGGCATTGACTGGACGTGATATATGCGGAAGTGCAATTACTGGTTCTGGAAAGACTGCAGCTTTTTCGCTACCTACTTTGGAGAGGTTACTCTATCGACCCAAACGTGATCGTGCAATAAGAGTTTTAATTCTAACTCCAGCTAGAGAATTGGCAATCCAAGTTCACAGTATGAATAGAGAAACTTGCTCAATGTTTGCTGACAATCGTTGGACCTTTCCTGGGCTTTCAGAAAAGGAAGCAAGAGGCTGCCTTAAGATCGATCCTGATGTGGTTGTGGCCACTCCAGGACGCATGATCGATCATCTACGCAATTCCATGTCCGTAGACTTGGACGATTTAGCAAGTTTTAATACTTGA

Coding sequence (CDS)

ATGGCTAAGAGTTTTGTGTTCGATCCCCCCAGTGACGAGGAATTCGACCACTCCGATGCTGAAGAAGAAGAAGAAGATGAAGAACAAGAAGAACGAGAAGAGGAACAAGGAGGAAAAGAGGAGGAAGATGAACCCCTTTCTCGTCGTCGCACTGAGTCTCCTTGGGATTTTGCCTCTTACTCCGAGTCGGTGGCTGAAGAACATGCCCGTAGAAGCACTACTTCTGTTGATTTCAAAATTTCCAAACTGCTTGAGAAGCGCTCCTCACACGTCACTCCTACCGCGGCTGATGATGATGAGAGTTCGGAAGAGGAATCTGACAGACAAGAAGATTATAGACCGGAAGACGACGATGATGGAACTAGTAATGCTGGTGAGGGTAAGTCGTTTTTTGCGCCATCCGATGGGGCTTCCTTTCATGCTAATTCGTTCATGGAGCTTAACTTGTCGCGCCCACTGATTCGAGCATGTGAGGCTTTGGGATATGCAAAGCCGACGCCAATTCAGGCGGCTTGCATACCGTTGGCATTGACTGGACGTGATATATGCGGAAGTGCAATTACTGGTTCTGGAAAGACTGCAGCTTTTTCGCTACCTACTTTGGAGAGGTTACTCTATCGACCCAAACGTGATCGTGCAATAAGAGTTTTAATTCTAACTCCAGCTAGAGAATTGGCAATCCAAGTTCACAGTATGAATAGAGAAACTTGCTCAATGTTTGCTGACAATCGTTGGACCTTTCCTGGGCTTTCAGAAAAGGAAGCAAGAGGCTGCCTTAAGATCGATCCTGATGTGGTTGTGGCCACTCCAGGACGCATGATCGATCATCTACGCAATTCCATGTCCGTAGACTTGGACGATTTAGCAAGTTTTAATACTTGA

Protein sequence

MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASYSESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDGTSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMFADNRWTFPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLASFNT
Homology
BLAST of Carg27887 vs. NCBI nr
Match: KAG7022397.1 (DEAD-box ATP-dependent RNA helicase 28, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.0 bits (1421), Expect = 3.1e-153
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
           TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240

Query: 241 ADNRWTFPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLASFNT 294
           ADNRWTFPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLASFNT
Sbjct: 241 ADNRWTFPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLASFNT 293

BLAST of Carg27887 vs. NCBI nr
Match: KAG6592218.1 (DEAD-box ATP-dependent RNA helicase 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 482.6 bits (1241), Expect = 2.3e-132
Identity = 269/291 (92.44%), Postives = 271/291 (93.13%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
           TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 290

BLAST of Carg27887 vs. NCBI nr
Match: XP_022976908.1 (DEAD-box ATP-dependent RNA helicase 28 isoform X3 [Cucurbita maxima])

HSP 1 Score: 472.6 bits (1215), Expect = 2.4e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. NCBI nr
Match: XP_022976907.1 (DEAD-box ATP-dependent RNA helicase 28 isoform X2 [Cucurbita maxima])

HSP 1 Score: 472.6 bits (1215), Expect = 2.4e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. NCBI nr
Match: XP_022976906.1 (DEAD-box ATP-dependent RNA helicase 28 isoform X1 [Cucurbita maxima])

HSP 1 Score: 472.6 bits (1215), Expect = 2.4e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. ExPASy Swiss-Prot
Match: Q9ZRZ8 (DEAD-box ATP-dependent RNA helicase 28 OS=Arabidopsis thaliana OX=3702 GN=RH28 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.0e-73
Identity = 180/316 (56.96%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFD--------------HSDAEEEE--EDEE-QEEREEEQGGKEEED 60
           M  SF F+  SD+E +                +AEE E  EDE+ +EE EEE   +EEED
Sbjct: 1   MPSSFFFEDASDDELELIRNQEDSSEEDVKEGEAEEHEAGEDEDGEEEYEEEDDDEEEED 60

Query: 61  EPLSR-RRTESPWDFASYSESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESS 120
           E   R    +SPWDFASYS SV EEHARR TTS+D KISK ++ R   ++    +++E  
Sbjct: 61  EKRKRDADAQSPWDFASYSSSVGEEHARRHTTSIDEKISKAIQHRPVPISINEEEEEEEE 120

Query: 121 EE-----ESDRQEDYRPEDDDDGTSNAGEG--KSFFAPSDGASFHANSFMELNLSRPLIR 180
           EE     E+D+QE+Y  EDD+       +   K FF+  DG SFHA++FMELNLSRPL+R
Sbjct: 121 EEDASDAETDKQEEYLSEDDEAAEYKPEDATPKPFFSTVDGVSFHADTFMELNLSRPLLR 180

Query: 181 ACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIR 240
           ACE LGY KPTPIQAACIPLALTGRD+C SAITGSGKTAAF+LPTLERLL+RPKR  A R
Sbjct: 181 ACETLGYKKPTPIQAACIPLALTGRDLCASAITGSGKTAAFALPTLERLLFRPKRVFATR 240

Query: 241 VLILTPARELAIQVHSMNRETCSMFADNR--WTFPGLSEKEARGCLKIDPDVVVATPGRM 290
           VLILTP RELA+Q+HSM  +  + F D +      GLS +E    L+  PD+VVATPGRM
Sbjct: 241 VLILTPTRELAVQIHSM-IQNLAQFTDIKCGLIVGGLSVREQEVVLRSMPDIVVATPGRM 300

BLAST of Carg27887 vs. ExPASy Swiss-Prot
Match: Q0INC5 (DEAD-box ATP-dependent RNA helicase 28 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0481100 PE=2 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 6.7e-66
Identity = 165/318 (51.89%), Postives = 188/318 (59.12%), Query Frame = 0

Query: 48  RRRTESPWDFASYSESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAAD-----DDESS 107
           R+  +SPW+F+SY+ESVA EHARR TTS+D KIS+ L        P+  D     D++ S
Sbjct: 26  RKPAQSPWEFSSYAESVAAEHARRRTTSIDEKISQALSGSRRGGKPSIPDGDSEGDEDDS 85

Query: 108 EEESDRQEDYR----------------PEDDDDGT-----------SNAGEGKS------ 167
           E E D +ED +                 EDDD+G               GE KS      
Sbjct: 86  EVEDDSEEDDKEVVEGEIDDEEDEVEESEDDDEGVEVSDEEVEELEEGKGEEKSDEVEEG 145

Query: 168 ------------------------------------FFAPSDGASFHANSFMELNLSRPL 227
                                               FFA S+GASFHANSF+ELNLSRPL
Sbjct: 146 EEGQDGEEEEKEEGDEEAAEEEEETDKKSGVVDPSKFFASSEGASFHANSFLELNLSRPL 205

Query: 228 IRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLERLLYRPKRDRA 287
           +RACEALGY KPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLP LERLL+RPKR  A
Sbjct: 206 LRACEALGYQKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPVLERLLFRPKRVPA 265

Query: 288 IRVLILTPARELAIQVHSMNRETCSMFADNRWTF--PGLSEKEARGCLKIDPDVVVATPG 290
           IRVLILTP RELA QVHSM  E  + F D R      GLS K     L+  PD+VVATPG
Sbjct: 266 IRVLILTPTRELAAQVHSM-IEKLAQFTDIRCCLIVGGLSTKVQEVALRSMPDIVVATPG 325

BLAST of Carg27887 vs. ExPASy Swiss-Prot
Match: P0C2N7 (ATP-dependent RNA helicase DRS1 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=DRS1 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.3e-37
Identity = 94/200 (47.00%), Postives = 125/200 (62.50%), Query Frame = 0

Query: 89  SHVTPTAADDDESSEEESDRQEDYRPEDDDDGTSNAGEGKSFFAPSD----GASFHANSF 148
           S  TP    DDE+SE+          +D++D    A   + F AP +    G     +SF
Sbjct: 229 SVATPVQHPDDEASED----------DDEEDAEEEARRKEFFAAPEETENVGKKGGLSSF 288

Query: 149 MELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLERL 208
             ++LSRP++R   ++G+ KPTPIQA  IP+AL G+D+ G A+TGSGKTAAF +P LERL
Sbjct: 289 QGMSLSRPILRGLTSVGFTKPTPIQAKTIPIALMGKDVVGGAVTGSGKTAAFVVPILERL 348

Query: 209 LYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMFADNRWTFP--GLSEKEARGCLKID 268
           LYRPK+    RV++LTP RELAIQ HS+  +  S   D ++     GLS K   G L++ 
Sbjct: 349 LYRPKKVPTTRVVVLTPTRELAIQCHSVATKLAS-HTDIKFCLAVGGLSLKVQEGELRLR 408

Query: 269 PDVVVATPGRMIDHLRNSMS 283
           PDVV+ATPGR IDH+RNS S
Sbjct: 409 PDVVIATPGRFIDHMRNSAS 417

BLAST of Carg27887 vs. ExPASy Swiss-Prot
Match: Q4I830 (ATP-dependent RNA helicase DRS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=DRS1 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.8e-34
Identity = 108/271 (39.85%), Postives = 156/271 (57.56%), Query Frame = 0

Query: 18  SDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASYSESVAEEHARRSTTSVD 77
           SDAEE  +++E+++ EE++ G E ED+           + AS ++SVA         + D
Sbjct: 174 SDAEESGDEKEEDDEEEQESGVEVEDDD----------EAASDNDSVATPTNHPDDDASD 233

Query: 78  FKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDGTSNAGEGKSFFAPSDGA 137
                            A+DD+E +EEE+ R+  + PE++++    A             
Sbjct: 234 -----------------ASDDEEDAEEEAKRKAFFAPEEENNPDKKA------------- 293

Query: 138 SFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFS 197
              A+SF  ++LSRP++R   ++G+ KPTPIQA  IP+AL G+D+ G A TGSGKT AF 
Sbjct: 294 --TASSFQTMSLSRPIMRGLASVGFTKPTPIQAKSIPIALMGKDVVGGAETGSGKTGAFI 353

Query: 198 LPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMFADNRWTFP--GLSEKEA 257
           LP LERLLYRPK+    RV++L P RELAIQ H++  +  + F D ++T    GLS K  
Sbjct: 354 LPILERLLYRPKKIPTTRVVVLLPTRELAIQCHAVATKLAA-FTDIKFTLAVGGLSLKAQ 401

Query: 258 RGCLKIDPDVVVATPGRMIDHLRNSMSVDLD 287
              LK+ PDV++ATPGR IDH+RNS S  +D
Sbjct: 414 ELELKLRPDVIIATPGRFIDHMRNSASFSVD 401

BLAST of Carg27887 vs. ExPASy Swiss-Prot
Match: A7TJM9 (ATP-dependent RNA helicase DRS1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) OX=436907 GN=DRS1 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.4e-33
Identity = 103/278 (37.05%), Postives = 153/278 (55.04%), Query Frame = 0

Query: 17  HSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASYSESVAEEHARRSTTSV 76
           H +  EEEE+EE+EE EEE    EEE+E L+            +     +++ +      
Sbjct: 134 HIEVAEEEEEEEEEEEEEE----EEEEEELA---------MDGFGMGATKKNEQEEAGDE 193

Query: 77  DFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDGTSNAGEGKSFFAP--- 136
           D                   DDD   EEE +  +D   +D++     A E  SF+AP   
Sbjct: 194 D-------------------DDDNEEEEEEEVDDDKEDDDEEREEDTAEEMASFYAPESE 253

Query: 137 -SDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGK 196
            S+  S   ++F  L LSRP+++    LGY KP+PIQ+A IP+ L+G+DI   A+TGSGK
Sbjct: 254 SSEATSTVHSTFNSLTLSRPVLKGLSDLGYTKPSPIQSATIPIGLSGKDIIAGAVTGSGK 313

Query: 197 TAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMFADNRW--TFPGL 256
           TAAF +P +ERLLY+P +  + RV++LTP RELAIQ+  + ++     +   +     GL
Sbjct: 314 TAAFMIPIIERLLYKPAKVASTRVIVLTPTRELAIQIADVGKKIGKYVSGLTFGLAVGGL 373

Query: 257 SEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDL 289
           + ++    LK  PD+V+ATPGR IDH+RNS S ++D +
Sbjct: 374 NLRQQEQELKTRPDIVIATPGRFIDHVRNSSSFNVDSV 379

BLAST of Carg27887 vs. ExPASy TrEMBL
Match: A0A6J1IH00 (DEAD-box ATP-dependent RNA helicase 28 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477138 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. ExPASy TrEMBL
Match: A0A6J1INI2 (DEAD-box ATP-dependent RNA helicase 28 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111477138 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. ExPASy TrEMBL
Match: A0A6J1II66 (DEAD-box ATP-dependent RNA helicase 28 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477138 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 266/291 (91.41%), Postives = 269/291 (92.44%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
            SNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 ASNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. ExPASy TrEMBL
Match: A0A6J1F9Y6 (DEAD-box ATP-dependent RNA helicase 28 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443445 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 265/291 (91.07%), Postives = 268/291 (92.10%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGK EEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKGEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
           TSNA EGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 TSNACEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. ExPASy TrEMBL
Match: A0A6J1FFD3 (DEAD-box ATP-dependent RNA helicase 28 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443445 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 265/291 (91.07%), Postives = 268/291 (92.10%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFDHSDAEEEEEDEEQEEREEEQGGKEEEDEPLSRRRTESPWDFASY 60
           MAKSFVFDPPSDEEFDHSDA EEEEDEEQEEREEEQGGK EEDEPLSRRRTESPWDFASY
Sbjct: 1   MAKSFVFDPPSDEEFDHSDA-EEEEDEEQEEREEEQGGKGEEDEPLSRRRTESPWDFASY 60

Query: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESSEEESDRQEDYRPEDDDDG 120
           SESVAEEHARRSTTSVDFKISKLLEKRSS+VTPTAADDDESSEEESDRQEDYRPEDDDDG
Sbjct: 61  SESVAEEHARRSTTSVDFKISKLLEKRSSNVTPTAADDDESSEEESDRQEDYRPEDDDDG 120

Query: 121 TSNAGEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180
           TSNA EGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR
Sbjct: 121 TSNACEGKSFFAPSDGASFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGR 180

Query: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRETCSMF 240
           DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM  E  + F
Sbjct: 181 DICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSM-IEKLAQF 240

Query: 241 ADNRWTF--PGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDLA 290
            D R      GLS KE    L+  PDVVVATPGRMIDHLRNSMSVDLDDLA
Sbjct: 241 TDIRCCLIVGGLSRKEQEAALRSMPDVVVATPGRMIDHLRNSMSVDLDDLA 289

BLAST of Carg27887 vs. TAIR 10
Match: AT4G16630.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.4e-74
Identity = 180/316 (56.96%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 1   MAKSFVFDPPSDEEFD--------------HSDAEEEE--EDEE-QEEREEEQGGKEEED 60
           M  SF F+  SD+E +                +AEE E  EDE+ +EE EEE   +EEED
Sbjct: 1   MPSSFFFEDASDDELELIRNQEDSSEEDVKEGEAEEHEAGEDEDGEEEYEEEDDDEEEED 60

Query: 61  EPLSR-RRTESPWDFASYSESVAEEHARRSTTSVDFKISKLLEKRSSHVTPTAADDDESS 120
           E   R    +SPWDFASYS SV EEHARR TTS+D KISK ++ R   ++    +++E  
Sbjct: 61  EKRKRDADAQSPWDFASYSSSVGEEHARRHTTSIDEKISKAIQHRPVPISINEEEEEEEE 120

Query: 121 EE-----ESDRQEDYRPEDDDDGTSNAGEG--KSFFAPSDGASFHANSFMELNLSRPLIR 180
           EE     E+D+QE+Y  EDD+       +   K FF+  DG SFHA++FMELNLSRPL+R
Sbjct: 121 EEDASDAETDKQEEYLSEDDEAAEYKPEDATPKPFFSTVDGVSFHADTFMELNLSRPLLR 180

Query: 181 ACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIR 240
           ACE LGY KPTPIQAACIPLALTGRD+C SAITGSGKTAAF+LPTLERLL+RPKR  A R
Sbjct: 181 ACETLGYKKPTPIQAACIPLALTGRDLCASAITGSGKTAAFALPTLERLLFRPKRVFATR 240

Query: 241 VLILTPARELAIQVHSMNRETCSMFADNR--WTFPGLSEKEARGCLKIDPDVVVATPGRM 290
           VLILTP RELA+Q+HSM  +  + F D +      GLS +E    L+  PD+VVATPGRM
Sbjct: 241 VLILTPTRELAVQIHSM-IQNLAQFTDIKCGLIVGGLSVREQEVVLRSMPDIVVATPGRM 300

BLAST of Carg27887 vs. TAIR 10
Match: AT5G60990.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 95.9 bits (237), Expect = 5.7e-20
Identity = 59/162 (36.42%), Postives = 84/162 (51.85%), Query Frame = 0

Query: 143 SFMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLE 202
           +F EL +   L++ACE LG+  P+ IQA  +P AL G+D+ G A TGSGKT AF++P L+
Sbjct: 10  TFAELGVREELVKACERLGWKNPSKIQAEALPFALEGKDVIGLAQTGSGKTGAFAIPILQ 69

Query: 203 RLL-----YRPKR----DRAIRVLILTPARELAIQVHSMNRE-------TCSMFADNRWT 262
            LL       PK+    D A    +L+P RELAIQ+              C++       
Sbjct: 70  ALLEYVYDSEPKKGRRPDPAFFACVLSPTRELAIQIAEQFEALGADISLRCAVLVG---- 129

Query: 263 FPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSVDLDDL 289
             G+   +    L   P V+VATPGR+ DH+ ++    L  L
Sbjct: 130 --GIDRMQQTIALGKRPHVIVATPGRLWDHMSDTKGFSLKSL 165

BLAST of Carg27887 vs. TAIR 10
Match: AT1G16280.1 (RNA helicase 36 )

HSP 1 Score: 88.6 bits (218), Expect = 9.2e-18
Identity = 60/173 (34.68%), Postives = 84/173 (48.55%), Query Frame = 0

Query: 119 DGTSNAGEGKSFFAPSDGA-SFHANSFMELNLSRPLIRACEALGYAKPTPIQAACIPLAL 178
           D   N  + + F  P+  + +  A +F  L L+   +  C+ LG  KPTP+Q  C+P  L
Sbjct: 34  DSDQNTPQFEKFTNPNPSSDTTSATNFEGLGLAEWAVETCKELGMRKPTPVQTHCVPKIL 93

Query: 179 TGRDICGSAITGSGKTAAFSLPTLERLLYRPKRDRAIRVLILTPARELAIQVHSMNRE-- 238
            GRD+ G A TGSGKTAAF+LP L RL   P     +  L++TP RELA Q+    +   
Sbjct: 94  AGRDVLGLAQTGSGKTAAFALPILHRLAEDP---YGVFALVVTPTRELAFQLAEQFKALG 153

Query: 239 -----TCSMFADNRWTFPGLSEKEARGCLKIDPDVVVATPGRMIDHLRNSMSV 284
                 CS+         G+        L   P +V+ TPGR+   L N+  V
Sbjct: 154 SCLNLRCSVIVG------GMDMLTQTMSLVSRPHIVITTPGRIKVLLENNPDV 197

BLAST of Carg27887 vs. TAIR 10
Match: AT5G63120.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-17
Identity = 55/138 (39.86%), Postives = 80/138 (57.97%), Query Frame = 0

Query: 144 FMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLER 203
           F + N    ++ A   LG+ +PTPIQA   P+AL GRD+ G A TGSGKT A+ LP L  
Sbjct: 167 FQDANFPDNILEAIAKLGFTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPALVH 226

Query: 204 LLYRPK--RDRAIRVLILTPARELAIQVHSMNRETCSMFADNRWT--FPGLSEKEARGCL 263
           +  +P+  +D    VLIL P RELA+Q+   +R+   + +  R T  + G  +      L
Sbjct: 227 VSAQPRLGQDDGPIVLILAPTRELAVQIQEESRK-FGLRSGVRSTCIYGGAPKGPQIRDL 286

Query: 264 KIDPDVVVATPGRMIDHL 278
           +   ++V+ATPGR+ID L
Sbjct: 287 RRGVEIVIATPGRLIDML 303

BLAST of Carg27887 vs. TAIR 10
Match: AT5G63120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-17
Identity = 55/138 (39.86%), Postives = 80/138 (57.97%), Query Frame = 0

Query: 144 FMELNLSRPLIRACEALGYAKPTPIQAACIPLALTGRDICGSAITGSGKTAAFSLPTLER 203
           F + N    ++ A   LG+ +PTPIQA   P+AL GRD+ G A TGSGKT A+ LP L  
Sbjct: 167 FQDANFPDNILEAIAKLGFTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPALVH 226

Query: 204 LLYRPK--RDRAIRVLILTPARELAIQVHSMNRETCSMFADNRWT--FPGLSEKEARGCL 263
           +  +P+  +D    VLIL P RELA+Q+   +R+   + +  R T  + G  +      L
Sbjct: 227 VSAQPRLGQDDGPIVLILAPTRELAVQIQEESRK-FGLRSGVRSTCIYGGAPKGPQIRDL 286

Query: 264 KIDPDVVVATPGRMIDHL 278
           +   ++V+ATPGR+ID L
Sbjct: 287 RRGVEIVIATPGRLIDML 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022397.13.1e-153100.00DEAD-box ATP-dependent RNA helicase 28, partial [Cucurbita argyrosperma subsp. a... [more]
KAG6592218.12.3e-13292.44DEAD-box ATP-dependent RNA helicase 28, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022976908.12.4e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X3 [Cucurbita maxima][more]
XP_022976907.12.4e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X2 [Cucurbita maxima][more]
XP_022976906.12.4e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZRZ82.0e-7356.96DEAD-box ATP-dependent RNA helicase 28 OS=Arabidopsis thaliana OX=3702 GN=RH28 P... [more]
Q0INC56.7e-6651.89DEAD-box ATP-dependent RNA helicase 28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P0C2N72.3e-3747.00ATP-dependent RNA helicase DRS1 OS=Chaetomium globosum (strain ATCC 6205 / CBS 1... [more]
Q4I8301.8e-3439.85ATP-dependent RNA helicase DRS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 1... [more]
A7TJM94.4e-3337.05ATP-dependent RNA helicase DRS1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IH001.2e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1INI21.2e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X3 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1II661.2e-12991.41DEAD-box ATP-dependent RNA helicase 28 isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1F9Y62.2e-12891.07DEAD-box ATP-dependent RNA helicase 28 isoform X3 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1FFD32.2e-12891.07DEAD-box ATP-dependent RNA helicase 28 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT4G16630.11.4e-7456.96DEA(D/H)-box RNA helicase family protein [more]
AT5G60990.15.7e-2036.42DEA(D/H)-box RNA helicase family protein [more]
AT1G16280.19.2e-1834.68RNA helicase 36 [more]
AT5G63120.21.2e-1739.86P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G63120.11.2e-1739.86P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 17..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..131
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..117
NoneNo IPR availablePANTHERPTHR24031RNA HELICASEcoord: 44..272
NoneNo IPR availablePANTHERPTHR24031:SF729BNAA01G17110D PROTEINcoord: 44..272
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 161..292
e-value: 7.4E-4
score: 28.8
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 173..293
score: 14.797091
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 94..291
e-value: 1.1E-41
score: 144.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 139..281
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 166..283
e-value: 1.3E-23
score: 83.7
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 142..170
score: 11.640392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27887-RACarg27887-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005730 nucleolus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity