Carg27881 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27881
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	vacuolar protein sorting-associated protein 27-like isoform X2
Location	Carg_Chr11: 3589262 .. 3594237 (-)
RNA-Seq Expression	Carg27881
Synteny	Carg27881

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTATTTATTATAAAGAGTTGCAGCGCCAGTTCTTGAGGAGATCCGCTACCAGTTTCCCTTCTGAGCTCTGCAAAACGGAGCATTTGACTGAGTTCAGCGGTAGGAGTATCACCGCGCTCCGGCGGATAAGACGGTGGTTTCTTGCAGTCAAGATTAATCGGAAGCATCGATAGAAGAGTTGGGGAATCGAGAGGATCGAGCGGCGGAGATCACAAAAATGTTGGCGGAGCCTCCTCCTTTCCAGGAAGCAGCGCGCTGTGAAGTCTGCAAATGCAGTTTCAACACCTTCCGACGACGGGTACCAAATTCTGTTTGCTCTATGATCGACTTCGTTGCATTACTTTAGATTGAGCTAATGCAACGATGATCAATTTCGATTCTGTTTCGTGCTTTCGAGCTTTCGATCGCGAGATTACATGATTTTCTGTGTTTTTATCTTTGTTTACAGCATCATTGCCGATGCTGTGGAAGGACGTTGTGCCACGAGCATTCTTCTTTTCAAATGGTATTATTGATTCCTCTTCCTCGTTGATGCCGTAATGGAGAATTTTTTGCGTTCTGACTGGTGTATGTATGTACTTGATTTGTGCGAAATTCAGGCCTTGCCGCAGTTCGATATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTCAATAACTCCTCTCAGTAAGTCATGTTAAAATTTAATCATACTCCCTGCCGTAGTTTATAACTTCAAAAGTTATTCCGAATCTGGTATTGGAATTGAAGTTCAAGCGAAGTCTTTTGAGACGATCTATGGTCATACTAGAAAGGATTTTATCGAGCTGAAGTTTTACTTCTCAATTGATAACTCATTTCGTTCCGAAGCTCAGAATTAAAGTGCGTGAGTTGAAACTGAAATAGAAAGTTGGCATGAGATATTTTCTTCTATTTAGTCTCAAATATTTTCTTGCAACTTCTTGAACTTGAACTTTCTTGTTGGAAGTTTCATGTTCTAGCTTTTAAACGACTAGAAACTTAAAAATCCTAACTGGGATCCGACGTTCTTGTATCAAGGATTTAGGTTGTACATGAATGTGAGATCTCACATTGGTTGGAGAGGGGAACGAACCATTTCTTACAAGGATGTAAAAATCTCTTCCTAACATACGTTTTAAAACTGTTAGGCTGACGGCAATATATAATGGGCCAAAGCAAACAATATCTTCTAGCGGTGAGGCTGGGCTGTTACAAATGGTATCAGAGCCAGACATCGGGTGGTGTGCCAGCAAGGACCCTGAGCCTTTAAGGGAGTGAATTGTGAGATCCCATATCAGTTGGAGAGGAGAACAAAACATTCCTTATAAGGTGTGGGAAACCTCTCCCCAACAGACACGTTTTTAAACCGTGAGGTTAACGGCGATACGTAACGGGTCAAAGCAAATAATATTTGTTAGTGGTGAACTTGAATTGTTATAGAGAAAGAATCCAAAACTTTTGAATTTTTGGAACTGGTCTATATTCAAGAAAAGTTAGAACCTGTAGTCCTTGTCTCTTGAAGCCGGGGCCTAAACGCATGTTGTTCTTGAAGTCTGTGACTCTTTTATATTCTATTTCTTGTTTCTTTAGCTTTCTTGTGAAGACACCATTAAAATTTTCTCATACCCACTTTCATCGATAATGAAATTTCCAGCTCCGAATCATTTTTCTCTACAAATCACTGCGATCGAAATAAACATCCTAAAACATTCTTTGAACACTCTTTAAAGGCTCAAAGAATTTCAAAACCACCAATAATGTGGTAGTTGGATGTTAGTGAGTGATATGAAGGCTTTTGCCTGTTGAATGAAGCATAAAAGCTCTTAGATCATCCATCAGCTCCTTCCTCTTGGAATTAGGCATAGAAAAAAAATTATATACAGACTTGGAAAGTTCTTGCTTTCTCTCGTTTGCTCCTAAATTCCTGCTCTCGTCGTAGACGCATCCTTAGAGTTTCCAAATTTTATGCTGAGATTGAACTTATTGTTTACTGTTTAGCACCTGTCCTATTTTTGAAAAACTAGCGTCTTATTTATCGAGTTTGCCCTATTACAGATCTAGAGAAGTTGATCGACCACCGTCGTTAGCTGTAGTCGATCAAGTTGCAGATACAGTTTCTCAATTAAACTTTGATGGGAATGTAAATTCTGAGCCTAAGTCAGCTGTGGAGCATCAACCTGGCGTTGAGCATTTCAGATGCAATTGTGGAATGCCATTGTGCATTTTGTGTCGCTCCAGTCCAGTCTATGAAAACAGAATCTGTTCCTTGCAGGTTTTAATGTTTAGTAATTTCTTATGCTCCTTGTGTATCAACCGTTGTCCCTTATGGAACTTGATTATTCTTTTTGGCAGAAGAAACAGTTTCCACCTCTACTCGGAACAATTCAAAAGCAAAGCAGACTGGATTCCAATCCTAAGGAATAGAGGATCTACCTCCAACAGCAAGCTTCGGTATGTTATCTGGAGTTGTTTTTATTCCAATAGGTTAGATTCTGTCTTCCCCTTTCTGGCCAGTATATGGAAAAGTAAAAGTTTTATCACACTCCTTCAAATAGTAAGTTTATCACAATCCAAATATAATTAATAAAATGATCAAATAACGTTAGTATAAAATTAAAGCTATGAATACTGTATTATAGCGTTAAAAAAATGCTACAATAAGTTAAAAGGTAAAAGATAGCATAATATTTTAGCTATATTCTACCTATTATAGCTTTAATAAAACGCTATGAAATATTTTTATAACAACTTATATAAGCTATAGTTTTATACTATATTATAGCATCTATTATGGCTTTAAAAAAAACGCTATAAACAGTCATCTATTATAGCTTTAAAAAAACGCTATAAAAAGTCCACAATAGCGTTTTTCGTATGCTATCGTATCCATTATTTCTTATAATGATGACTTTGGTCCAGTGACACATTTTCTCATTGCTTACATTCTTGCGTATGTATTAGGTATCAAATTACCCTCCTTTATCATTGTTGGTATTATTGATTTGGTATATAAGTCCGATCTTAATCTGCAGTTGTAATTTATATTATTGAATAGGAATCCTGCAGATGGACATCAAGGATTCATTATGAAAGCAAAGTGGCGAGTATATTATTGTGTATGATAATTTTTTTTTTTTGGTGTCTATCAAAGGCTTTTAAATTCTGCTTCATCTCATGAATTTCATGTTCTTGCAGGGGTTGAGGGAAAGCAATCAGAAACGGTGACACTCATGCTGTCAAGAAACTTTTGGTGAGGTACTAAATGCCTTTATGAGAAAATGAAAATATGTAATGGAATTATTTACTGTTCTCATGTTGGCAGGGTGTAGATGCAAATTACATGACAAGCAAGGATTGTCTTTGCTGCATGTGGTAGAATCTCTCTCTCTTCCTCCCACCCCATTAGAATATGTCGTGATATCGATTTAAAGTTATGATATTAAGACTAGTATTGATTTGGATGTAGGCGGCCGTCTTTAATCAGACTGATATTGTTTTTGCCCTCATTGAAAGTGGTGCTAGCCTGGACTACAAAAATGCTCAAGGTAACTTCTTGTCACTATTTCTTTATGCGAATGCTTTTGGTCGGGACTTTGGGAATTGGGAGTGTTTAACAAACTGTCTTGAAAATAGAAACTCTTCTGAGAACAAATTTCAAACGACCCTTGCTTATCAGTTCTATCCCCCTTCTCTTTTGTCAAGCTTGGCGATTAAAGCAAAGAATAACTGTAGCAAATTTCTTTGTGATTGTGTGAGATCCACGGGTGTGGAAACCGGTGTGGAAACCTCTCTCTAGTAAACGCGTTTTAGAAACTTTGAAGGGAAGTCCAAAAGGGAAATCCCAAGGAGGACAATATCTATTAACGGTGAGTTTGGATTGTTAGAAATAGTATCAGAGCCAGATACCAGACAATGTGCCAGCGAGGAGGCTGCGTCTCGAAGGAGGATGGACACGAGGCGGTGTGCCAACAAGGACGCTTGGTCTCGAAGGGGGTGGATTTGGTGGTCCCACATCGATTAGAGAAGGAAACGAGTGTCAGCGAGCGAGAACGCTAGGTCTCAAAGAGGGGTGGATTATGAGATCTCATATCGGTTGGGGAGGAGAACAAAACATTTTTTATAAGGGTGTGAAAATCTCTCCCTAGTAGACGCATTTTAAAAAACCTTGAGGCGAAACTCGAAAGGGAAAGTTCAAAAGAGAATAATATCTACTAGCGGTGAGCTTGAGCTGTTATTACACTTACTACAACGAGTAGAAAGCCACGAAGTATGGGTAGCTTTCTAAATTACAAGCGACTTGAAATGATTGGTTAACGCGATCCTTATTATTTTCGTCCTTCAAACCACAATCCTTGGGTAGAAAAGGGAAACTCGTATTGGACCTCTTTCTAAATTTTGACTTAAAAATCTAATTTTTCTGGAAAGTGAGGTTTCATGTTAGAAGTGTGAGAATACTTTGCATAATGGTCATTGGTTTCCACGGTTTACAGGAAAAACGCCATTGGATTGTTGGCTCCAGTTGTTTGCAATAATAAGATGCGAGAGAGATGGGAGGAGAAGTGGAAGAAAAGCAACAGCCCACATCACCTAATATTTAGTAAATACTTCTATCTTTCTGCACCACCGCGGCCTTTATTTTTTTAATTTTTAAGGAACACGCATGGAGAATTTTGAAACTAATTGAGAAACTAATATAGATTAGGTTTGGTTATATCGTCGATGGATTGGTTGATGGATTCTTTGAATCGAAGGTTGACCAAGTTGAGTTCAACAACTTGTGGGTGGGGTGTGGCGTGATTCAAATCTTTTACCTTCTGGTGATACGTGTTACTCGTTGAGTTGTGCTTAAATATTCCAAAGTTGTTGTGTTATATTTGTCAATAATTTTAGTAGAAAGTTCATTGAACACGCCACACAATGACTAGACAATGCTTACGTGGTAAATTTTAAATAGCGTGACAAAATTGAACTTT

mRNA sequence

ATTTATTTATTATAAAGAGTTGCAGCGCCAGTTCTTGAGGAGATCCGCTACCAGTTTCCCTTCTGAGCTCTGCAAAACGGAGCATTTGACTGAGTTCAGCGGTAGGAGTATCACCGCGCTCCGGCGGATAAGACGGTGGTTTCTTGCAGTCAAGATTAATCGGAAGCATCGATAGAAGAGTTGGGGAATCGAGAGGATCGAGCGGCGGAGATCACAAAAATGTTGGCGGAGCCTCCTCCTTTCCAGGAAGCAGCGCGCTGTGAAGTCTGCAAATGCAGTTTCAACACCTTCCGACGACGGCATCATTGCCGATGCTGTGGAAGGACGTTGTGCCACGAGCATTCTTCTTTTCAAATGGCCTTGCCGCAGTTCGATATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTCAATAACTCCTCTCAATCTAGAGAAGTTGATCGACCACCGTCGTTAGCTGTAGTCGATCAAGTTGCAGATACAGTTTCTCAATTAAACTTTGATGGGAATGTAAATTCTGAGCCTAAGTCAGCTGTGGAGCATCAACCTGGCGTTGAGCATTTCAGATGCAATTGTGGAATGCCATTGTGCATTTTGTGTCGCTCCAGTCCAGTCTATGAAAACAGAATCTGTTCCTTGCAGAAGAAACAGTTTCCACCTCTACTCGGAACAATTCAAAAGCAAAGCAGACTGGATTCCAATCCTAAGGAATAGAGGATCTACCTCCAACAGCAAGCTTCGGTATGTTATCTGGAGTTGTTTTTATTCCAATAGGAATTTCATGTTCTTGCAGGGGTTGAGGGAAAGCAATCAGAAACGGTGACACTCATGCTGTCAAGAAACTTTTGGTGAGGGTGTAGATGCAAATTACATGACAAGCAAGGATTGTCTTTGCTGCATGTGGCGGCCGTCTTTAATCAGACTGATATTGTTTTTGCCCTCATTGAAAGTGGTGCTAGCCTGGACTACAAAAATGCTCAAGGAAAAACGCCATTGGATTGTTGGCTCCAGTTGTTTGCAATAATAAGATGCGAGAGAGATGGGAGGAGAAGTGGAAGAAAAGCAACAGCCCACATCACCTAATATTTAGTAAATACTTCTATCTTTCTGCACCACCGCGGCCTTTATTTTTTTAATTTTTAAGGAACACGCATGGAGAATTTTGAAACTAATTGAGAAACTAATATAGATTAGGTTTGGTTATATCGTCGATGGATTGGTTGATGGATTCTTTGAATCGAAGGTTGACCAAGTTGAGTTCAACAACTTGTGGGTGGGGTGTGGCGTGATTCAAATCTTTTACCTTCTGGTGATACGTGTTACTCGTTGAGTTGTGCTTAAATATTCCAAAGTTGTTGTGTTATATTTGTCAATAATTTTAGTAGAAAGTTCATTGAACACGCCACACAATGACTAGACAATGCTTACGTGGTAAATTTTAAATAGCGTGACAAAATTGAACTTT

Coding sequence (CDS)

ATGTTGGCGGAGCCTCCTCCTTTCCAGGAAGCAGCGCGCTGTGAAGTCTGCAAATGCAGTTTCAACACCTTCCGACGACGGCATCATTGCCGATGCTGTGGAAGGACGTTGTGCCACGAGCATTCTTCTTTTCAAATGGCCTTGCCGCAGTTCGATATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTCAATAACTCCTCTCAATCTAGAGAAGTTGATCGACCACCGTCGTTAGCTGTAGTCGATCAAGTTGCAGATACAGTTTCTCAATTAAACTTTGATGGGAATGTAAATTCTGAGCCTAAGTCAGCTGTGGAGCATCAACCTGGCGTTGAGCATTTCAGATGCAATTGTGGAATGCCATTGTGCATTTTGTGTCGCTCCAGTCCAGTCTATGAAAACAGAATCTGTTCCTTGCAGAAGAAACAGTTTCCACCTCTACTCGGAACAATTCAAAAGCAAAGCAGACTGGATTCCAATCCTAAGGAATAG

Protein sequence

MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVCADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCGMPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSNPKE

Homology

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BLAST of Carg27881 vs. NCBI nr
Match: KAG7022008.1 (VPS27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 352.1 bits (902), Expect = 2.7e-93
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC
Sbjct: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSNPKE 165
           MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSNPKE
Sbjct: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSNPKE 164

[Close]

BLAST of Carg27881 vs. NCBI nr
Match: KAG6588113.1 (Rabankyrin-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 259.2 bits (661), Expect = 2.4e-65
Identity = 125/161 (77.64%), Postives = 128/161 (79.50%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC
Sbjct: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPP LAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPLLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           MPLCI        +     LQKK          K  + DSN
Sbjct: 121 MPLCICVAPVQSMKTESVPLQKKTVSTSTQNNSKAKKTDSN 161

[Close]

BLAST of Carg27881 vs. NCBI nr
Match: XP_022929532.1 (vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moschata] >XP_022929540.1 vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moschata] >KAG7021998.1 Rabankyrin-5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.1 bits (658), Expect = 5.2e-65
Identity = 124/161 (77.02%), Postives = 128/161 (79.50%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           MPLCI        +     LQKK          K  + DSN
Sbjct: 121 MPLCICVAPVQSMKTESVPLQKKTASTSTQNNSKAKKTDSN 161

[Close]

BLAST of Carg27881 vs. NCBI nr
Match: XP_022929552.1 (potassium channel KOR2-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 258.1 bits (658), Expect = 5.2e-65
Identity = 124/161 (77.02%), Postives = 128/161 (79.50%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           MPLCI        +     LQKK          K  + DSN
Sbjct: 121 MPLCICVAPVQSMKTESVPLQKKTASTSTQNNSKAKKTDSN 161

[Close]

BLAST of Carg27881 vs. NCBI nr
Match: XP_022929548.1 (vacuolar protein sorting-associated protein 27-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 253.1 bits (645), Expect = 1.7e-63
Identity = 119/142 (83.80%), Postives = 122/142 (85.92%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQK 143
           MPLCI        +     LQK
Sbjct: 121 MPLCICVAPVQSMKTESVPLQK 142

[Close]

BLAST of Carg27881 vs. ExPASy Swiss-Prot
Match: Q810B6 (Rabankyrin-5 OS=Mus musculus OX=10090 GN=Ankfy1 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 3.8e-10
Identity = 27/65 (41.54%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 1    MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
            ML++ PP+ + + C  C   F    R+HHCR CGR LCH+ S+ ++ + +FD++  VRVC
Sbjct: 1097 MLSKEPPWCDGSNCYECTAKFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVC 1156

Query: 61   ADCFN 66
              CF+
Sbjct: 1157 NICFD 1161

[Close]

BLAST of Carg27881 vs. ExPASy Swiss-Prot
Match: Q9P2R3 (Rabankyrin-5 OS=Homo sapiens OX=9606 GN=ANKFY1 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 8.5e-10
Identity = 27/65 (41.54%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 1    MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
            ML++ PP+ + + C  C   F    R+HHCR CGR LCH+ S+ ++ + +FD++  VRVC
Sbjct: 1097 MLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVC 1156

Query: 61   ADCFN 66
              CF+
Sbjct: 1157 NICFD 1161

[Close]

BLAST of Carg27881 vs. ExPASy Swiss-Prot
Match: Q7QAJ2 (Lateral signaling target protein 2 homolog OS=Anopheles gambiae OX=7165 GN=AGAP003678 PE=3 SV=6)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 30/67 (44.78%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 1    MLAEPP---PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSV 60
            M  EPP   P  +A RC  C  +F  FRRRHHCR CG   C   S+    LP++ +  +V
Sbjct: 1089 MPEEPPRWIPDCDAPRCMACASAFTPFRRRHHCRNCGGVFCGVCSNLSKPLPKYGLTKAV 1148

Query: 61   RVCADCF 65
            RVC DC+
Sbjct: 1149 RVCRDCY 1155

[Close]

BLAST of Carg27881 vs. ExPASy Swiss-Prot
Match: Q17AN2 (Lateral signaling target protein 2 homolog OS=Aedes aegypti OX=7159 GN=AAEL005241 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 28/58 (48.28%), Postives = 34/58 (58.62%), Query Frame = 0


Query: 7   PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVCADCF 65
           P  +A RC  C  SF  FRRRHHCR CG   C   SS    LP++ +  +VRVC DC+
Sbjct: 849 PDGDAPRCMACASSFTPFRRRHHCRNCGGVFCGVCSSASAPLPKYGLTKAVRVCRDCY 906

[Close]

BLAST of Carg27881 vs. ExPASy Swiss-Prot
Match: Q6FQJ1 (Vacuolar protein sorting-associated protein 27 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=VPS27 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 5   PPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVCADCF 64
           P  + ++  C +C   F+   RRHHCR CG   C +HSS  + LP   I+ SVRVC +C+
Sbjct: 167 PADWIDSDACMICSKKFSLLNRRHHCRSCGGVFCQDHSSKSIPLPDLGIYDSVRVCDNCY 226

Query: 65  NN 67
           ++
Sbjct: 227 DD 228

[Close]

BLAST of Carg27881 vs. ExPASy TrEMBL
Match: A0A6J1EUN7 (vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.5e-65
Identity = 124/161 (77.02%), Postives = 128/161 (79.50%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           MPLCI        +     LQKK          K  + DSN
Sbjct: 121 MPLCICVAPVQSMKTESVPLQKKTASTSTQNNSKAKKTDSN 161

[Close]

BLAST of Carg27881 vs. ExPASy TrEMBL
Match: A0A6J1EN28 (potassium channel KOR2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.5e-65
Identity = 124/161 (77.02%), Postives = 128/161 (79.50%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           MPLCI        +     LQKK          K  + DSN
Sbjct: 121 MPLCICVAPVQSMKTESVPLQKKTASTSTQNNSKAKKTDSN 161

[Close]

BLAST of Carg27881 vs. ExPASy TrEMBL
Match: A0A6J1ESF6 (vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436069 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.2e-64
Identity = 119/142 (83.80%), Postives = 122/142 (85.92%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           ML EPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALP+FDIHSSVRVC
Sbjct: 1   MLVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPKFDIHSSVRVC 60

Query: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRCNCG 120
           ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    CNCG
Sbjct: 61  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDCNCG 120

Query: 121 MPLCILCRSSPVYENRICSLQK 143
           MPLCI        +     LQK
Sbjct: 121 MPLCICVAPVQSMKTESVPLQK 142

[Close]

BLAST of Carg27881 vs. ExPASy TrEMBL
Match: A0A6J1HTP4 (vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467386 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 6.9e-63
Identity = 124/164 (75.61%), Postives = 127/164 (77.44%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDI +SVRVC
Sbjct: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIRASVRVC 60

Query: 61  ADCFNNSSQSREVDRP---PSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRC 120
           ADCFNNSSQSREV RP   PSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    C
Sbjct: 61  ADCFNNSSQSREVGRPPSLPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDC 120

Query: 121 NCGMPLCILCRSSPVYENRICSLQKKQFPPLLGTIQKQSRLDSN 162
           NCGMPLCI        E     LQKK          K  + DSN
Sbjct: 121 NCGMPLCICVAPVQSTETESVPLQKKTASTSTQNNSKAKKTDSN 164

[Close]

BLAST of Carg27881 vs. ExPASy TrEMBL
Match: A0A6J1HTR8 (vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467386 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.2e-61
Identity = 119/145 (82.07%), Postives = 121/145 (83.45%), Query Frame = 0


Query: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSSVRVC 60
           MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDI +SVRVC
Sbjct: 1   MLAEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIRASVRVC 60

Query: 61  ADCFNNSSQSREVDRP---PSLAVVDQVADTVSQLNFDGNVNSEPKSAVEHQPGVEHFRC 120
           ADCFNNSSQSREV RP   PSLAVVDQVADTVSQLN DGNVNSEPKSAVEHQP +    C
Sbjct: 61  ADCFNNSSQSREVGRPPSLPSLAVVDQVADTVSQLNLDGNVNSEPKSAVEHQPALSISDC 120

Query: 121 NCGMPLCILCRSSPVYENRICSLQK 143
           NCGMPLCI        E     LQK
Sbjct: 121 NCGMPLCICVAPVQSTETESVPLQK 145

[Close]

BLAST of Carg27881 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 26/91 (28.57%), Postives = 41/91 (45.05%), Query Frame = 0


Query: 7   PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHS------SVRVC 66
           P Q    C  C C F    RRHHCR CGR  C + ++  +     D+ +       +RVC
Sbjct: 38  PDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGKCTANSIPFAPSDLRTPREDWERIRVC 97

Query: 67  ADCFNNSSQSREVDRPPSLAVVDQVADTVSQ 92
             CF    Q    D  P ++ + +++ + S+
Sbjct: 98  NYCFRQWEQG---DGGPHVSNITELSTSPSE 125

[Close]

BLAST of Carg27881 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 46.2 bits (108), Expect = 2.9e-05
Identity = 23/69 (33.33%), Postives = 30/69 (43.48%), Query Frame = 0


Query: 7   PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSS------VRVC 66
           P Q    C  C   F  F RRHHCR CGR  C + ++  +  P  +   S      +RVC
Sbjct: 35  PDQSCPVCYECDAQFTVFNRRHHCRLCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVC 94

Query: 67  ADCFNNSSQ 70
             C+    Q
Sbjct: 95  NYCYKQWEQ 103

[Close]

BLAST of Carg27881 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 46.2 bits (108), Expect = 2.9e-05
Identity = 23/69 (33.33%), Postives = 30/69 (43.48%), Query Frame = 0


Query: 7   PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSS------VRVC 66
           P Q    C  C   F  F RRHHCR CGR  C + ++  +  P  +   S      +RVC
Sbjct: 35  PDQSCPVCYECDAQFTVFNRRHHCRLCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVC 94

Query: 67  ADCFNNSSQ 70
             C+    Q
Sbjct: 95  NYCYKQWEQ 103

[Close]

BLAST of Carg27881 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 46.2 bits (108), Expect = 2.9e-05
Identity = 23/69 (33.33%), Postives = 30/69 (43.48%), Query Frame = 0


Query: 7   PFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFDIHSS------VRVC 66
           P Q    C  C   F  F RRHHCR CGR  C + ++  +  P  +   S      +RVC
Sbjct: 35  PDQSCPVCYECDAQFTVFNRRHHCRLCGRVFCAKCAANSIPSPSDETKDSHEEPERIRVC 94

Query: 67  ADCFNNSSQ 70
             C+    Q
Sbjct: 95  NYCYKQWEQ 103

The following BLAST results are available for this feature:

BLAST of Carg27881 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022008.1	2.7e-93	100.00	VPS27 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6588113.1	2.4e-65	77.64	Rabankyrin-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022929532.1	5.2e-65	77.02	vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita moscha...	[more]
XP_022929552.1	5.2e-65	77.02	potassium channel KOR2-like isoform X3 [Cucurbita moschata]	[more]
XP_022929548.1	1.7e-63	83.80	vacuolar protein sorting-associated protein 27-like isoform X2 [Cucurbita moscha...	[more]

BLAST of Carg27881 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q810B6	3.8e-10	41.54	Rabankyrin-5 OS=Mus musculus OX=10090 GN=Ankfy1 PE=1 SV=2	[more]
Q9P2R3	8.5e-10	41.54	Rabankyrin-5 OS=Homo sapiens OX=9606 GN=ANKFY1 PE=1 SV=2	[more]
Q7QAJ2	2.5e-09	44.78	Lateral signaling target protein 2 homolog OS=Anopheles gambiae OX=7165 GN=AGAP0...	[more]
Q17AN2	3.2e-09	48.28	Lateral signaling target protein 2 homolog OS=Aedes aegypti OX=7159 GN=AAEL00524...	[more]
Q6FQJ1	3.2e-09	40.32	Vacuolar protein sorting-associated protein 27 OS=Candida glabrata (strain ATCC ...	[more]

BLAST of Carg27881 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EUN7	2.5e-65	77.02	vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita mosc...	[more]
A0A6J1EN28	2.5e-65	77.02	potassium channel KOR2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1ESF6	8.2e-64	83.80	vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita mosc...	[more]
A0A6J1HTP4	6.9e-63	75.61	vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita maxi...	[more]
A0A6J1HTR8	2.2e-61	82.07	vacuolar protein sorting-associated protein 27-like isoform X2 OS=Cucurbita maxi...	[more]

BLAST of Carg27881 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14270.1	1.3e-05	28.57	phosphatidylinositol-4-phosphate 5-kinase family protein	[more]
AT4G33240.1	2.9e-05	33.33	1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos...	[more]
AT4G33240.2	2.9e-05	33.33	1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos...	[more]
AT4G33240.3	2.9e-05	33.33	1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000306	FYVE zinc finger	SMART	SM00064	fyve_4	coord: 2..69 e-value: 3.7E-16 score: 69.7
IPR000306	FYVE zinc finger	PFAM	PF01363	FYVE	coord: 9..67 e-value: 1.1E-14 score: 54.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..81 e-value: 6.0E-18 score: 66.3
None	No IPR available	PANTHER	PTHR47794	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27	coord: 4..138
None	No IPR available	CDD	cd15760	FYVE_scVPS27p_like	coord: 7..64 e-value: 1.32197E-18 score: 73.0999
IPR017455	Zinc finger, FYVE-related	PROSITE	PS50178	ZF_FYVE	coord: 8..68 score: 11.573293
IPR011011	Zinc finger, FYVE/PHD-type	SUPERFAMILY	57903	FYVE/PHD zinc finger	coord: 8..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27881-RA	Carg27881-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006623	protein targeting to vacuole
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0033565	ESCRT-0 complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0032266	phosphatidylinositol-3-phosphate binding
molecular_function	GO:0043130	ubiquitin binding