Carg27748 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27748
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	espin-like
Location	Carg_Chr18: 4263155 .. 4263649 (-)
RNA-Seq Expression	Carg27748
Synteny	Carg27748

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTGTACGAGGAGAAAAAACAGTTATACAAGCTAGTGTTGAGGGGAGAATGGAGGCGTCTGCTGGAGGTATGTAAGGGTGCGAGGCAGTTAGTGTTTCCGATCACTAGCAATCGAGACACGGCGTTTCATTTGGCAGTGTACAGTGGAAAAAAAGAACCTTTAAAAACAATTCTTGAGCAGGTGATGGAGATGGATTACATAGAGCATTGGAAGAGCTACGTTGAAAACACACCTCTACATGAAGCTGCCACTGTGGGGAATTTGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAAGCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGATCGTTGAATATATTTTGAATGAATGTGAAGATTATTACACTCGATCTCCCCTAAATTGGATGGCAGATGGTACTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGGTTAG

mRNA sequence

ATGGGTCTGTACGAGGAGAAAAAACAGTTATACAAGCTAGTGTTGAGGGGAGAATGGAGGCGTCTGCTGGAGGTATGTAAGGGTGCGAGGCAGTTAGTGTTTCCGATCACTAGCAATCGAGACACGGCGTTTCATTTGGCAGTGTACAGTGGAAAAAAAGAACCTTTAAAAACAATTCTTGAGCAGGTGATGGAGATGGATTACATAGAGCATTGGAAGAGCTACGTTGAAAACACACCTCTACATGAAGCTGCCACTGTGGGGAATTTGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAAGCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGATCGTTGAATATATTTTGAATGAATGTGAAGATTATTACACTCGATCTCCCCTAAATTGGATGGCAGATGGTACTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGGTTAG

Coding sequence (CDS)

ATGGGTCTGTACGAGGAGAAAAAACAGTTATACAAGCTAGTGTTGAGGGGAGAATGGAGGCGTCTGCTGGAGGTATGTAAGGGTGCGAGGCAGTTAGTGTTTCCGATCACTAGCAATCGAGACACGGCGTTTCATTTGGCAGTGTACAGTGGAAAAAAAGAACCTTTAAAAACAATTCTTGAGCAGGTGATGGAGATGGATTACATAGAGCATTGGAAGAGCTACGTTGAAAACACACCTCTACATGAAGCTGCCACTGTGGGGAATTTGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAAGCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGATCGTTGAATATATTTTGAATGAATGTGAAGATTATTACACTCGATCTCCCCTAAATTGGATGGCAGATGGTACTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGGTTAG

Protein sequence

MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTILEQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG

Homology

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BLAST of Carg27748 vs. NCBI nr
Match: KAG7012618.1 (Espin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.4 bits (877), Expect = 2.1e-90
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL
Sbjct: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR
Sbjct: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG 165
           YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG
Sbjct: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG 164

[Close]

BLAST of Carg27748 vs. NCBI nr
Match: XP_022954460.1 (espin-like [Cucurbita moschata])

HSP 1 Score: 329.3 bits (843), Expect = 1.9e-86
Identity = 158/163 (96.93%), Postives = 158/163 (96.93%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL
Sbjct: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVMEMDY EHWKS  ENTPLHEAAT GNLDAFKLLVEFRKEDLL LNKRGETPLYRAAR
Sbjct: 61  EQVMEMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLALNKRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF
Sbjct: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 163

[Close]

BLAST of Carg27748 vs. NCBI nr
Match: XP_022954414.1 (espin-like [Cucurbita moschata])

HSP 1 Score: 295.4 bits (755), Expect = 3.0e-76
Identity = 143/163 (87.73%), Postives = 150/163 (92.02%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M +YEEKKQLYKL LRGEWR L EVCK A QLV PI ++ +TA HLAVYSGK EPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDASQLVLPINNSGETALHLAVYSGKGEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVMEMDYIE+WKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR
Sbjct: 61  EQVMEMDYIEYWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YGQLQIVEYILNECEDYY+RSPLNWM DG TPIIHEAIQSE+F
Sbjct: 121 YGQLQIVEYILNECEDYYSRSPLNWMVDG-TPIIHEAIQSESF 162

[Close]

BLAST of Carg27748 vs. NCBI nr
Match: KAG6573477.1 (Espin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 291.6 bits (745), Expect = 4.3e-75
Identity = 141/171 (82.46%), Postives = 150/171 (87.72%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M +YEEKKQLYKL LRGEWR L EVCK ARQL+ PI ++ +TA HLAV+SGK+EPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 60

Query: 61  EQVM-------EMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGET 120
           EQ++       EMDY EHWKS  ENTPLHEAAT GNLDAFKLLVEFRKEDLL LNKRGET
Sbjct: 61  EQLVLPINNSGEMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLALNKRGET 120

Query: 121 PLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG 165
           PLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG
Sbjct: 121 PLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFG 171

[Close]

BLAST of Carg27748 vs. NCBI nr
Match: XP_023523820.1 (uncharacterized protein LOC111787944 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.0 bits (733), Expect = 1.1e-73
Identity = 135/163 (82.82%), Postives = 147/163 (90.18%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M LY+E+KQL+KL LRGEW+ L +VCKGARQLV PI ++ +TA HLAVYSGK+EPLKTIL
Sbjct: 1   MDLYDERKQLHKLALRGEWKNLDKVCKGARQLVLPINNSGETALHLAVYSGKEEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVME +Y EHWKS  ENTPLHEAAT GNLDAFKLLVEFRKEDLLELN RGETPLYRAA+
Sbjct: 61  EQVMEENYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLELNSRGETPLYRAAK 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YGQLQIVEYILNECEDYY+RSP NWM DGTTPIIHEAIQSENF
Sbjct: 121 YGQLQIVEYILNECEDYYSRSPRNWMGDGTTPIIHEAIQSENF 163

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BLAST of Carg27748 vs. ExPASy Swiss-Prot
Match: Q9ET47 (Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.3e-07
Identity = 39/118 (33.05%), Postives = 55/118 (46.61%), Query Frame = 0


Query: 42  TAFHLAVYSGKKEPLKTILEQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRK 101
           T  HLA   G  + +K +L Q      I    +     P+H AA  G+L + KLLV    
Sbjct: 106 TVLHLAARFGHPDVVKWLLYQGGANSAI---TTDTGALPIHYAAAKGDLPSLKLLVGHYP 165

Query: 102 EDLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQ 160
           E +      G TPLY A + G L++ +Y++ EC    +  P     DG TP +H A Q
Sbjct: 166 EGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----SADPHLRAQDGMTP-LHAAAQ 215

[Close]

BLAST of Carg27748 vs. ExPASy Swiss-Prot
Match: B1AK53 (Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.2e-07
Identity = 31/81 (38.27%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 80  PLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAARYGQLQIVEYILNEC-EDYY 139
           P+H AA  G+  + +LLVE   E +    K G TPLY A + G L++ +Y++ EC  D +
Sbjct: 141 PIHYAAAKGDFPSLRLLVEHYPEGVNAQTKNGATPLYLACQEGHLEVTQYLVQECGADPH 200

Query: 140 TRSPLNWMADGTTPIIHEAIQ 160
            R+      DG TP +H A Q
Sbjct: 201 ARA-----HDGMTP-LHAAAQ 215

[Close]

BLAST of Carg27748 vs. ExPASy Swiss-Prot
Match: Q63618 (Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-06
Identity = 30/80 (37.50%), Postives = 42/80 (52.50%), Query Frame = 0


Query: 80  PLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYT 139
           P+H AA  G+L + KLLV    E +      G TPLY A + G L++ +Y++ EC    +
Sbjct: 141 PIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----S 200

Query: 140 RSPLNWMADGTTPIIHEAIQ 160
             P     DG TP +H A Q
Sbjct: 201 ADPHLRAQDGMTP-LHAAAQ 215

[Close]

BLAST of Carg27748 vs. ExPASy Swiss-Prot
Match: Q99NH0 (Ankyrin repeat domain-containing protein 17 OS=Mus musculus OX=10090 GN=Ankrd17 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.4e-06
Identity = 35/111 (31.53%), Postives = 57/111 (51.35%), Query Frame = 0


Query: 27   KGARQLVFPITSNRDTAFHLAVYSGKKEPLKTILEQVMEMDYIEHWKSYVENTPLHEAAT 86
            KGA     P+ S+RDTA  +A   G  +  + ++ +   +D     ++   NTPL  AA 
Sbjct: 1303 KGADVNAPPVPSSRDTALTIAADKGHYKFCELLIGKGAHIDV----RNKKGNTPLWLAAN 1362

Query: 87   VGNLDAFKLLVEFRKEDLLELNKRGETPLYRAARYGQLQIVEYILNECEDY 138
             G+LD  +LLV+    D+   + R  TPL  A R G +++V Y++ E   +
Sbjct: 1363 GGHLDVVQLLVQ-ATADVDAADNRKITPLMAAFRKGHVKVVRYLVKEVNQF 1408

[Close]

BLAST of Carg27748 vs. ExPASy Swiss-Prot
Match: Q5ZLC8 (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C OS=Gallus gallus OX=9031 GN=ANKRD52 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 5.7e-06
Identity = 31/90 (34.44%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 42  TAFHLAVYSGKKEPLKTILEQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRK 101
           TA H AV+SG  E +  +L +   +   +      +  P+H AA +G+L+  KLLV  R 
Sbjct: 142 TALHHAVHSGHLEMVNLLLNKGASLSTCDK----KDRQPIHWAAFLGHLEVLKLLVA-RG 201

Query: 102 EDLLELNKRGETPLYRAARYGQLQIVEYIL 132
            D++  +K+G T L+ AA  GQ+++V ++L
Sbjct: 202 ADVMCKDKKGYTLLHTAAASGQIEVVRHLL 226

[Close]

BLAST of Carg27748 vs. ExPASy TrEMBL
Match: A0A6J1GSG9 (espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456725 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 9.0e-87
Identity = 158/163 (96.93%), Postives = 158/163 (96.93%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL
Sbjct: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVMEMDY EHWKS  ENTPLHEAAT GNLDAFKLLVEFRKEDLL LNKRGETPLYRAAR
Sbjct: 61  EQVMEMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLALNKRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF
Sbjct: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 163

[Close]

BLAST of Carg27748 vs. ExPASy TrEMBL
Match: A0A6J1GSX9 (espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456674 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 143/163 (87.73%), Postives = 150/163 (92.02%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M +YEEKKQLYKL LRGEWR L EVCK A QLV PI ++ +TA HLAVYSGK EPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDASQLVLPINNSGETALHLAVYSGKGEPLKTIL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
           EQVMEMDYIE+WKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR
Sbjct: 61  EQVMEMDYIEYWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YGQLQIVEYILNECEDYY+RSPLNWM DG TPIIHEAIQSE+F
Sbjct: 121 YGQLQIVEYILNECEDYYSRSPLNWMVDG-TPIIHEAIQSESF 162

[Close]

BLAST of Carg27748 vs. ExPASy TrEMBL
Match: A0A6J1K0P4 (espin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490547 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.9e-62
Identity = 120/163 (73.62%), Postives = 133/163 (81.60%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M LY+EKK LYK  LR +W  + EVCK ARQ V PI +  DTA HLAV+SGK+EPLKT L
Sbjct: 1   MDLYKEKKALYKFALREQWEYVGEVCKDARQFVLPINNGGDTALHLAVHSGKEEPLKTFL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
            QVMEM+Y EHWKSYVENTPLHEAA+VGNL A KLLVE RKEDLLE+N  GETPLYRAAR
Sbjct: 61  AQVMEMEYTEHWKSYVENTPLHEAASVGNLGAVKLLVELRKEDLLEVNIWGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YG+L IV+YILN+CED+Y RS LNW A G TPIIH AIQSENF
Sbjct: 121 YGKLDIVQYILNDCEDFYARSSLNWTAKG-TPIIHAAIQSENF 162

[Close]

BLAST of Carg27748 vs. ExPASy TrEMBL
Match: A0A6J1GQZ1 (ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC111456726 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.7e-61
Identity = 119/163 (73.01%), Postives = 136/163 (83.44%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M L ++ K+ Y L +RGEW  L +VCK A +LV PIT + DT+ HLAV+SGK+EPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
            QVME++Y  +WKS+ ENTPLHEAATVGNL A KLLVE RKEDLLE+N RGETPLYRAAR
Sbjct: 61  AQVMEIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YG+L+IVEYILNECEDYYTRSPLNWMA G TPIIH AIQSENF
Sbjct: 121 YGKLEIVEYILNECEDYYTRSPLNWMAKG-TPIIHAAIQSENF 162

[Close]

BLAST of Carg27748 vs. ExPASy TrEMBL
Match: A0A6J1GR15 (espin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.7e-61
Identity = 119/163 (73.01%), Postives = 136/163 (83.44%), Query Frame = 0


Query: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60
           M L ++ K+ Y L +RGEW  L +VCK A +LV PIT + DT+ HLAV+SGK+EPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 61  EQVMEMDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGETPLYRAAR 120
            QVME++Y  +WKS+ ENTPLHEAATVGNL A KLLVE RKEDLLE+N RGETPLYRAAR
Sbjct: 61  AQVMEIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGETPLYRAAR 120

Query: 121 YGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENF 164
           YG+L+IVEYILNECEDYYTRSPLNWMA G TPIIH AIQSENF
Sbjct: 121 YGKLEIVEYILNECEDYYTRSPLNWMAKG-TPIIHAAIQSENF 162

[Close]

BLAST of Carg27748 vs. TAIR 10
Match: AT3G54070.1 (Ankyrin repeat family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 37/126 (29.37%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 10  LYKLVLRGEWRRLLE-VCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTILEQVMEMDY 69
           +YK VL G+W+     + +    +V  IT N + A H+AV +  K+ ++ +L ++   D 
Sbjct: 54  MYKAVLTGDWKTASTLISRKECNVVEQITGNSEIALHIAVAAKHKDFVRNLLREMDPPDL 113

Query: 70  IEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLEL-NKRGETPLYRAARYGQLQIV 129
               K+   NTPL  AA +G+++  ++L+   + DL ++ N++  TP++ AA YG  ++V
Sbjct: 114 --SLKNKDGNTPLSFAAALGDIETAEMLINMIR-DLPDISNEKTMTPIHIAALYGHGEMV 173

Query: 130 EYILNE 134
           +Y+ ++
Sbjct: 174 QYLFSK 176

[Close]

BLAST of Carg27748 vs. TAIR 10
Match: AT5G20350.1 (Ankyrin repeat family protein with DHHC zinc finger domain )

HSP 1 Score: 49.7 bits (117), Expect = 2.6e-06
Identity = 35/113 (30.97%), Postives = 57/113 (50.44%), Query Frame = 0


Query: 45  HLAVYSGKKEPLKTILEQVMEMDYIEHWKSYVEN---TPLHEAATVGNLDAFKLLVEF-R 104
           H A Y G  + ++ +L       +++ ++   +    TPLH AA  GNL+A  +LV+  +
Sbjct: 170 HWAAYKGFADSIRLLL-------FLDAYRGRQDKEGCTPLHWAAIRGNLEACTVLVQAGK 229

Query: 105 KEDLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPI 154
           KEDL+  +K G TP   AA     Q+  ++ N       RS L    DG++P+
Sbjct: 230 KEDLMITDKTGLTPAQLAAEKNHRQVSFFLGN------ARSLLEKRCDGSSPL 269

[Close]

BLAST of Carg27748 vs. TAIR 10
Match: AT3G04710.1 (ankyrin repeat family protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 36/115 (31.30%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 47  AVYSGKKEPLKTILEQVME----MDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKE 106
           A  +G  E LK + +Q+ E       +E  K   +   LH AA  G  +  + L+E  K 
Sbjct: 21  AACTGNLEFLKNVAKQLDEGKDLTKTVESIKDANKRGALHFAAREGQTEICRYLLEELKL 80

Query: 107 DLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEA 158
           +    ++ G+TPL  AAR GQ++ V+Y+L +  D    S L     G T + H A
Sbjct: 81  NADAKDETGDTPLVHAARQGQIETVKYLLEQGADPNIASEL-----GATALHHAA 130

[Close]

BLAST of Carg27748 vs. TAIR 10
Match: AT3G04710.2 (ankyrin repeat family protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 36/115 (31.30%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 47  AVYSGKKEPLKTILEQVME----MDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKE 106
           A  +G  E LK + +Q+ E       +E  K   +   LH AA  G  +  + L+E  K 
Sbjct: 21  AACTGNLEFLKNVAKQLDEGKDLTKTVESIKDANKRGALHFAAREGQTEICRYLLEELKL 80

Query: 107 DLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEA 158
           +    ++ G+TPL  AAR GQ++ V+Y+L +  D    S L     G T + H A
Sbjct: 81  NADAKDETGDTPLVHAARQGQIETVKYLLEQGADPNIASEL-----GATALHHAA 130

[Close]

BLAST of Carg27748 vs. TAIR 10
Match: AT3G04710.3 (ankyrin repeat family protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 36/115 (31.30%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 47  AVYSGKKEPLKTILEQVME----MDYIEHWKSYVENTPLHEAATVGNLDAFKLLVEFRKE 106
           A  +G  E LK + +Q+ E       +E  K   +   LH AA  G  +  + L+E  K 
Sbjct: 245 AACTGNLEFLKNVAKQLDEGKDLTKTVESIKDANKRGALHFAAREGQTEICRYLLEELKL 304

Query: 107 DLLELNKRGETPLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEA 158
           +    ++ G+TPL  AAR GQ++ V+Y+L +  D    S L     G T + H A
Sbjct: 305 NADAKDETGDTPLVHAARQGQIETVKYLLEQGADPNIASEL-----GATALHHAA 354

The following BLAST results are available for this feature:

BLAST of Carg27748 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012618.1	2.1e-90	100.00	Espin, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022954460.1	1.9e-86	96.93	espin-like [Cucurbita moschata]	[more]
XP_022954414.1	3.0e-76	87.73	espin-like [Cucurbita moschata]	[more]
KAG6573477.1	4.3e-75	82.46	Espin, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023523820.1	1.1e-73	82.82	uncharacterized protein LOC111787944 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg27748 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ET47	2.3e-07	33.05	Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2	[more]
B1AK53	5.2e-07	38.27	Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1	[more]
Q63618	1.5e-06	37.50	Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2	[more]
Q99NH0	3.4e-06	31.53	Ankyrin repeat domain-containing protein 17 OS=Mus musculus OX=10090 GN=Ankrd17 ...	[more]
Q5ZLC8	5.7e-06	34.44	Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C OS=Ga...	[more]

BLAST of Carg27748 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSG9	9.0e-87	96.93	espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456725 PE=4 SV=1	[more]
A0A6J1GSX9	1.4e-76	87.73	espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456674 PE=4 SV=1	[more]
A0A6J1K0P4	5.9e-62	73.62	espin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490547 PE=4 SV=1	[more]
A0A6J1GQZ1	1.7e-61	73.01	ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1GR15	1.7e-61	73.01	espin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1	[more]

BLAST of Carg27748 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54070.1	1.1e-09	29.37	Ankyrin repeat family protein	[more]
AT5G20350.1	2.6e-06	30.97	Ankyrin repeat family protein with DHHC zinc finger domain	[more]
AT3G04710.1	3.4e-06	31.30	ankyrin repeat family protein	[more]
AT3G04710.2	3.4e-06	31.30	ankyrin repeat family protein	[more]
AT3G04710.3	3.4e-06	31.30	ankyrin repeat family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 39..68 e-value: 3.3 score: 16.7 coord: 110..139 e-value: 0.37 score: 19.9 coord: 76..106 e-value: 1.6 score: 17.7
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 110..142 score: 9.64448
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 3..162 e-value: 3.2E-21 score: 77.7
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 32..147
None	No IPR available	PFAM	PF13637	Ank_4	coord: 77..131 e-value: 2.0E-9 score: 37.8
None	No IPR available	PANTHER	PTHR24177	CASKIN	coord: 20..128
None	No IPR available	PANTHER	PTHR24177:SF331	REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED	coord: 20..128
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 110..132 score: 9.303788

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27748-RA	Carg27748-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding