Carg27727 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27727
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCarboxypeptidase
LocationCarg_Chr05: 4320013 .. 4322915 (-)
RNA-Seq ExpressionCarg27727
SyntenyCarg27727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAACTATTAGTTCTTCGTTTCTGTTTTGTTCTCATTGCTTCCTCTTCTCTGTTTCATGTCGGAGCTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAGTAAGCCAAATTCATTAATTTTCCTTCTATTTTATTTTAGTCATTATTTTTTATATATTTGTTCTTGGGATTGTGTTGTTCAGAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCCAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTTTGGTTACAGGGCGGACCGGTGAGCAGCCGACCTTTTCCAACGAATTATCTATAAATTTTGTGTACTTCGTAAGAAAATGGCTCAAATATTGACAAACAGGGAGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATTGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGTCAACTCCTTTTCCTTTTAATTTTCACCATTTTAACCTTAAATTCTAATATTTTACACTAAATTTATGATTTTAACATTAAAAAAAAAAAAAAAACAGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCAAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGTGCCATCCAAGCTGGCAATTTGACTCTTAATATCGGAGGTACTTTTATTTTTACTTTCTTTTTTAATAAAAAGTTTCAAATATAAATGATGAATTATAACTTTTTTTGTTATATGTAAAAAAAAAAAATCATATTATAACTTTGATTTGTTTAAAATGATCACATTGTAATAATATAGAATCAATAATTTAAATACACGACTCTCTTGATTTTGTTTGCCAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGGTTAGTATTCTCATTCTCTCAATCTCATCCAAAAATTTTATCAATTTTTTTGATATTTTCGTTTTCAGAATGATAAACGAAAAAAATACTCCTAAACTACGAAGTTGATTTCAATTATATCCTGATCTAAGAGTTAAAATGAAATATCTCAAAGCTCGAGAGTATAACTGAAACAAACCTCATATTTCAAGAGTATTCTTTTATTATTTATCCTTAACACAATAATCTTTGTCTAAAATTTTACAGTCTTCTTGGGGTCCTCTTCTCAAAGATCTCTCACGAATCGACGACATGACGCTGCAAAAATCAAATAGGTTAATATTTTTCACTTTTTTTTTTTTAATATAAACATTAATAAAGAAGTGAAATATATTCTATAAATATAAAAGTTATATGTAACAAAAGCTGTCTTTTGAAAATCAGTTTAGCGTTGAAAATCAAGCAGCAAGTTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTCGAGGGATTAATTTTCTCCAATAGCAACCACGTGGTACGTTTTGTTGTTATTATTATTATTATTATGAAAAAAAATCCTCCCAAACCAATGAAATTTAAATTTAAATTTATATTAACTGGAGTCTTTATTTTTATTATTTTTTTTTATTAAGTTATCTTTTAAATTAGTAAATTTTTTATGCTTCATTCATAACTATTTAATTTTTTAAGTTTATAAAAACTATTTTGACCTACGAATTCCTTTTATTTTATCATCCACTTAAAACATGTTTTTAAAATATAAACTCAGTTCTAAAAAAATATTATATCTTTTTTTGAGAGAACGAACATTAGTTTCAAAAAGACATAAAACGAAATGATTATCGATATGTTATAACGAGTCATTTTTGCAGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCACTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATATATACAAACCCGGAGCTAAAGGAGACTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCATCAAATGTCACGTACGTACCAATAAACCCCATTTTCTTTAAATAAAATAATAATCATTAAAACTCTTAAGACATAAAAATTAAATTAATTATCTTGGAGATTAAGTCCTACCATTTTAGCCTTAAAAACTAAAATATAAATTTTGTATGTATAGATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACTCGAGATTTTATGAAGCCAAGGATCAACGAGGTGTTTTTTTTAACCAAACTATTTTTAATATTTTTATTTGTGGATTATTTTTTGCTTCATAATATAACATTCAATTATGTCCTAATATTTTTATTTTTTCCAAAAGGTGGATGAGTTACTGGCTAAAGGTGTTAATGTGACGGTCTACAACGGTCAAGTAATTATTTTTTTTTCCTTTTTTCAAATTAATAATTTCAAACAAAAAAACAAACAATATTTTGAAGTGATAAAAATAGTAATATTCTCCTTGATTTATATAGGTCGATCTTATTTGTTCAACCAAGGGAACAGAAGCGTGGATTAATAAGCTCAAGTAATTTGTTTCCAAGTAATTTATGTTATGGACATGAACACAAACACGTCTTCCTAAAAAAAAATATCCATTTTTTTTTCTTTCGAATTTAGGTGGAAATGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAAGGAGAAGCACACCAAAGGTTTCACCAAGTCACATAATAAATCTAGTGTCATTTGA

mRNA sequence

ATGGCTAAACTATTAGTTCTTCGTTTCTGTTTTGTTCTCATTGCTTCCTCTTCTCTGTTTCATGTCGGAGCTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCCAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTTTGGTTACAGGGCGGACCGGGAGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATTGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCAAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGTGCCATCCAAGCTGGCAATTTGACTCTTAATATCGGAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGTCTTCTTGGGGTCCTCTTCTCAAAGATCTCTCACGAATCGACGACATGACGCTGCAAAAATCAAATAGTTTAGCGTTGAAAATCAAGCAGCAAGTTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTCGAGGGATTAATTTTCTCCAATAGCAACCACGTGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCACTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATATATACAAACCCGGAGCTAAAGGAGACTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCATCAAATGTCACATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACTCGAGATTTTATGAAGCCAAGGATCAACGAGGTGGATGAGTTACTGGCTAAAGGTGTTAATGTGACGGTCTACAACGGTCAAGTCGATCTTATTTGTTCAACCAAGGGAACAGAAGCGTGGATTAATAAGCTCAAGTGGAAATGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAAGGAGAAGCACACCAAAGGTTTCACCAAGTCACATAATAAATCTAGTGTCATTTGA

Coding sequence (CDS)

ATGGCTAAACTATTAGTTCTTCGTTTCTGTTTTGTTCTCATTGCTTCCTCTTCTCTGTTTCATGTCGGAGCTTCCATAACTCACAAATCTCAAGACGGAACTGAAGAATGGGGCTACGTTCAAGTCAGACCCAAAGCCCATATGTTTTGGTGGCTTTATAGAAGTCCTTCCAGAGTGGACGTCGGTTCCAAGCCATGGCCAACCATTCTTTGGTTACAGGGCGGACCGGGAGGCTCGGGAACTGGGTTTGGGAACTTCCTGGAAATTGGGCCATTGAACTCGAACTTGAAGCCGAGAAACTCAACATGGCTGCAAAAAGCAGACCTCTTGTTTGTGGATAATCCAGTCGGGACCGGATATAGCTACGTTGAGAGTGGAGGTGAATTTGCAAAAGGCGATGTGGAGGCGGCGACCGACATGACGACTTTGCTGGCTGAGGTTTCGAACAACACTGTCGGCCTACGAAACACTCCTTTGTACATCTTTGCGGAGTCTTACGGTGGCAAATTCGCCGTCACTCTGGCTTTGTCCCTTCTTCGTGCCATCCAAGCTGGCAATTTGACTCTTAATATCGGAGGAGTTTCTCTAGGAAACAGTTGGATGTCTCCAGAAGATTACACGTCTTCTTGGGGTCCTCTTCTCAAAGATCTCTCACGAATCGACGACATGACGCTGCAAAAATCAAATAGTTTAGCGTTGAAAATCAAGCAGCAAGTTAAAAACAGGTTGTACGAAAATGCAACTCTTTCATGGAATGATCTCGAGGGATTAATTTTCTCCAATAGCAACCACGTGGATTTCTATAACTTCATGTTGGATTTTGAAATGGACTCATTAGCCGCGACTACGACGACAAATAGTGTGGAATCGAATAGAATTGATTCACTTGAAGTAAGAAAGAACTCTCCATATCTTAGTTTGAATATATACAAACCCGGAGCTAAAGGAGACTTGGAAGCTTTGATGAATGGTCCAATTAAGCAAAAACTCACTATTATCCCATCAAATGTCACATGGGGAGGTCAATCCGACCTAGTCTTCCAATTTTTTACTCGAGATTTTATGAAGCCAAGGATCAACGAGGTGGATGAGTTACTGGCTAAAGGTGTTAATGTGACGGTCTACAACGGTCAAGTCGATCTTATTTGTTCAACCAAGGGAACAGAAGCGTGGATTAATAAGCTCAAGTGGAAATGGCTAAAGGCATTTATAAGCAAAGAGAGAACTACTTTATTTTGTGGAAAGGAGAAGCACACCAAAGGTTTCACCAAGTCACATAATAAATCTAGTGTCATTTGA

Protein sequence

MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTKGFTKSHNKSSVI
Homology
BLAST of Carg27727 vs. NCBI nr
Match: KAG7029856.1 (Serine carboxypeptidase-like 51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 875.5 bits (2261), Expect = 1.8e-250
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK
Sbjct: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK
Sbjct: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN
Sbjct: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK
Sbjct: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420

Query: 421 GFTKSHNKSSVI 433
           GFTKSHNKSSVI
Sbjct: 421 GFTKSHNKSSVI 432

BLAST of Carg27727 vs. NCBI nr
Match: XP_023515401.1 (serine carboxypeptidase-like 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.0 bits (2148), Expect = 2.3e-237
Identity = 407/426 (95.54%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVLR CFVLIASS LFHVG SITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLRLCFVLIASSFLFHVGGSITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQ GNLTLN+GGVSLGNSWMSPEDYTSSWGPLLKDLSRIDD TLQKSNSLALKIKQQ+K
Sbjct: 181 AIQPGNLTLNLGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDTTLQKSNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDS+EVRK
Sbjct: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSVEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGAKG+LEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRIN
Sbjct: 301 NSPYLSLNVYKPGAKGELEALMNGPIKQKLTIIPPNVTWGGQSDLVFQFFTADFMKPRIN 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELL KG+NVTVY GQVDLICSTKGTE WINKLKW+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLVKGINVTVYGGQVDLICSTKGTETWINKLKWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. NCBI nr
Match: XP_022975190.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 812.4 bits (2097), Expect = 1.9e-231
Identity = 398/426 (93.43%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL  CFVLIASSSLFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT AWINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. NCBI nr
Match: XP_022985845.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 808.5 bits (2087), Expect = 2.7e-230
Identity = 395/426 (92.72%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL  CFVLIASSSLFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMD+LAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDTLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQS+LVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSNLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT+ WINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTQVWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. NCBI nr
Match: XP_022975497.1 (serine carboxypeptidase-like 51 [Cucurbita maxima])

HSP 1 Score: 808.5 bits (2087), Expect = 2.7e-230
Identity = 396/426 (92.96%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL  CFVLIASSSLF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT AWINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. ExPASy Swiss-Prot
Match: Q67Y83 (Serine carboxypeptidase-like 51 OS=Arabidopsis thaliana OX=3702 GN=SCPL51 PE=2 SV=2)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 232/403 (57.57%), Postives = 292/403 (72.46%), Query Frame = 0

Query: 30  SQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEI 89
           + DG+E WGYV+VRPKAHMFWW Y+SP RV+  SKPWP ILWLQGGPG SG G GNF E+
Sbjct: 29  NSDGSEAWGYVEVRPKAHMFWWHYKSPYRVENPSKPWPIILWLQGGPGASGVGIGNFQEV 88

Query: 90  GPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDVEAATDMTTLLAEV 149
           GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D EAA D+T LL ++
Sbjct: 89  GPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQL 148

Query: 150 SNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGVSLGNSWMSPEDYT 209
            N    L  +PL+I AESYGGK AV L LS++ A+Q+G L L++GGV LG+SW+SPED+ 
Sbjct: 149 FNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFV 208

Query: 210 SSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFSNSNHVDF 269
            SWGPLLK +SR+DD  L  SNSLA KIK Q+KN  Y  AT +W DLE LI S SN VDF
Sbjct: 209 FSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDLENLISSKSNFVDF 268

Query: 270 YNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYL----SLNIYKPGAKGDLEALMN 329
           YNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  +   +GDL+ LMN
Sbjct: 269 YNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDVE-DVEGDLDKLMN 328

Query: 330 GPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQVDLIC 389
           G IK+KL IIP+++ WG  SD VF      FMKP I +VDELLA GV+VT+YNGQ+D+IC
Sbjct: 329 GVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGVDVTIYNGQLDVIC 388

Query: 390 STKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTKGFTKSH 427
           ST GTEAW++KL+W+ L+ F   ER  LFC  ++ T+GFTKS+
Sbjct: 389 STSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSY 425

BLAST of Carg27727 vs. ExPASy Swiss-Prot
Match: Q920A5 (Retinoid-inducible serine carboxypeptidase OS=Mus musculus OX=10090 GN=Scpep1 PE=1 SV=2)

HSP 1 Score: 338.2 bits (866), Expect = 1.4e-91
Identity = 185/426 (43.43%), Postives = 252/426 (59.15%), Query Frame = 0

Query: 3   KLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVG 62
           ++ ++R   +L++    F  G++I  +  +G E W YV VR  AHMFWWLY + +     
Sbjct: 6   RICLVRLWLLLLSFLLGFSAGSAIDWREPEGKEVWDYVTVRKDAHMFWWLYYATNPCKNF 65

Query: 63  SKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSY 122
           S+  P ++WLQGGPGGS TGFGNF EIGPL++ LKPRN+TWLQ A LLFVDNPVGTG+SY
Sbjct: 66  SE-LPLVMWLQGGPGGSSTGFGNFEEIGPLDTQLKPRNTTWLQWASLLFVDNPVGTGFSY 125

Query: 123 VESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAI 182
           V +   +AK     A+DM  LL    +     +  P YIF+ESYGGK A  +++ L +A+
Sbjct: 126 VNTTDAYAKDLDTVASDMMVLLKSFFDCHKEFQTVPFYIFSESYGGKMAAGISVELYKAV 185

Query: 183 QAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNR 242
           Q G +  N  GV+LG+SW+SP D   SWGP L  +S +D+  L + + +A ++   V   
Sbjct: 186 QQGTIKCNFSGVALGDSWISPVDSVLSWGPYLYSMSLLDNQGLAEVSDIAEQVLDAVNKG 245

Query: 243 LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNS 302
            Y+ AT  W   E +I  N++ V+FYN +            T +S E     SLE  ++ 
Sbjct: 246 FYKEATQLWGKAEMIIEKNTDGVNFYNIL------------TKSSPEKAMESSLEFLRSP 305

Query: 303 PYLSLNIYKPGAKGD-LEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINE 362
                  +    +GD L  LMNGPIK+KL IIP +++WG Q+  VF     DFMKP I+ 
Sbjct: 306 LVRLCQRHVRHLQGDALSQLMNGPIKKKLKIIPEDISWGAQASYVFLSMEGDFMKPAIDV 365

Query: 363 VDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCG-KEKHTK 422
           VD+LLA GVNVTVYNGQ+DLI  T G E+W+ KLKW  L  F   +   L+   K   T 
Sbjct: 366 VDKLLAAGVNVTVYNGQLDLIVDTIGQESWVQKLKWPQLSKFNQLKWKALYTDPKSSETA 418

Query: 423 GFTKSH 427
            F KS+
Sbjct: 426 AFVKSY 418

BLAST of Carg27727 vs. ExPASy Swiss-Prot
Match: Q9HB40 (Retinoid-inducible serine carboxypeptidase OS=Homo sapiens OX=9606 GN=SCPEP1 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 187/408 (45.83%), Postives = 244/408 (59.80%), Query Frame = 0

Query: 21  HVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSG 80
           + GA I   +++G E W YV VR  A+MFWWLY + +     S+  P ++WLQGGPGGS 
Sbjct: 24  NAGAVIDWPTEEGKEVWDYVTVRKDAYMFWWLYYATNSCKNFSE-LPLVMWLQGGPGGSS 83

Query: 81  TGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDM 140
           TGFGNF EIGPL+S+LKPR +TWLQ A LLFVDNPVGTG+SYV   G +AK     A+DM
Sbjct: 84  TGFGNFEEIGPLDSDLKPRKTTWLQAASLLFVDNPVGTGFSYVNGSGAYAKDLAMVASDM 143

Query: 141 TTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGVSLGNSW 200
             LL    +     +  P YIF+ESYGGK A  + L L +AIQ G +  N  GV+LG+SW
Sbjct: 144 MVLLKTFFSCHKEFQTVPFYIFSESYGGKMAAGIGLELYKAIQRGTIKCNFAGVALGDSW 203

Query: 201 MSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFS 260
           +SP D   SWGP L  +S ++D  L + + +A ++   V   LY  AT  W   E +I  
Sbjct: 204 ISPVDSVLSWGPYLYSMSLLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQ 263

Query: 261 NSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYLSLNIYKPGAKGD-LE 320
           N++ V+FYN         L  +T T+++ES    SLE  ++        +    + D L 
Sbjct: 264 NTDGVNFYNI--------LTKSTPTSTMES----SLEFTQSHLVCLCQRHVRHLQRDALS 323

Query: 321 ALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQV 380
            LMNGPI++KL IIP + +WGGQ+  VF     DFMKP I+ VDELL  G+NVTVYNGQ+
Sbjct: 324 QLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQL 383

Query: 381 DLICSTKGTEAWINKLKWKWLKAFISKERTTLFCG-KEKHTKGFTKSH 427
           DLI  T G EAW+ KLKW  L  F   +   L+   K   T  F KS+
Sbjct: 384 DLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSY 418

BLAST of Carg27727 vs. ExPASy Swiss-Prot
Match: Q920A6 (Retinoid-inducible serine carboxypeptidase OS=Rattus norvegicus OX=10116 GN=Scpep1 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.9e-91
Identity = 189/426 (44.37%), Postives = 252/426 (59.15%), Query Frame = 0

Query: 3   KLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVG 62
           ++ ++R   +L++    F  G+++  + Q+G E W YV VR  A MFWWLY + +     
Sbjct: 6   RICLVRLWLLLLSFLLGFSAGSALNWREQEGKEVWDYVTVREDARMFWWLYYATNPCKNF 65

Query: 63  SKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSY 122
           S+  P ++WLQGGPGGS TGFGNF EIGPL++ LKPRN+TWLQ A LLFVDNPVGTG+SY
Sbjct: 66  SE-LPLVMWLQGGPGGSSTGFGNFEEIGPLDTRLKPRNTTWLQWASLLFVDNPVGTGFSY 125

Query: 123 VESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAI 182
           V +   +AK     A+DM  LL    +     +  P YIF+ESYGGK A  ++L L +AI
Sbjct: 126 VNTTDAYAKDLDTVASDMMVLLKSFFDCHKEFQTVPFYIFSESYGGKMAAGISLELHKAI 185

Query: 183 QAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNR 242
           Q G +  N  GV+LG+SW+SP D   SWGP L  +S +D+  L + + +A ++   V   
Sbjct: 186 QQGTIKCNFSGVALGDSWISPVDSVLSWGPYLYSVSLLDNKGLAEVSDIAEQVLNAVNKG 245

Query: 243 LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNS 302
            Y+ AT  W   E +I  N++ V+FYN         L  +T   S+ES    SLE  ++ 
Sbjct: 246 FYKEATQLWGKAEMIIEKNTDGVNFYNI--------LTKSTPDTSMES----SLEFFRSP 305

Query: 303 PYLSLNIYKPGAKGD-LEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINE 362
                  +    +GD L  LMNGPIK+KL IIP +V+WG QS  VF     DFMKP I+ 
Sbjct: 306 LVRLCQRHVRHLQGDALSQLMNGPIKKKLKIIPDDVSWGAQSSSVFISMEEDFMKPVIDI 365

Query: 363 VDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCG-KEKHTK 422
           VD LL  GVNVTVYNGQ+DLI  T G E+W+ KLKW  L  F   +   L+   K   T 
Sbjct: 366 VDTLLELGVNVTVYNGQLDLIVDTIGQESWVQKLKWPQLSRFNQLKWKALYTNPKSSETS 418

Query: 423 GFTKSH 427
            F KS+
Sbjct: 426 AFVKSY 418

BLAST of Carg27727 vs. ExPASy Swiss-Prot
Match: Q54DY7 (Serine carboxypeptidase S10 family member 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0291912 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.6e-26
Identity = 103/426 (24.18%), Postives = 183/426 (42.96%), Query Frame = 0

Query: 6   VLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKP 65
           +++  F++I+   + +V  S       G   +  V     A++F+  Y S +        
Sbjct: 1   MMKLLFIIISIIFVINVSNSTPTLQLSG---YFNVNETTNANLFYLFYESQNSPSTD--- 60

Query: 66  WPTILWLQGGPGGSGTGFGNFLEIGPL----NSNLKPRNSTWLQKADLLFVDNPVGTGYS 125
            P ILWL GGPG S      F E GP     N  L    ++W   A++L+VD+P+G G+S
Sbjct: 61  -PLILWLTGGPGCSSL-MAAFYENGPYFVNDNLTLSENPNSWNMVANVLYVDSPLGAGFS 120

Query: 126 YVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRA 185
           YV     ++  + E + ++ + L +  +        PLYIF ESY G +  + +  + + 
Sbjct: 121 YVVDSDGYSTTETEISENLYSFLTQFLSKYPKYSKLPLYIFGESYAGHYVPSFSYYIYQK 180

Query: 186 IQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKN 245
              G  T+N+ G+++GN  + P     S GP       +D   L+++  L    +Q + +
Sbjct: 181 -NLGLATINLKGLAIGNGMVDPYIQYGSLGPFAYAHGMLDINALKETEGLYESCQQAIDS 240

Query: 246 RLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKN 305
             Y   T   N++  ++   + + + Y+         ++ T   N        ++     
Sbjct: 241 GDYNMTTQICNNIMDIVQEYAGNFNVYD---------VSKTCYPNEPLCYNFTAI----- 300

Query: 306 SPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINE 365
             YL+L                   KQ   ++P N TW   S   +    RD+    IN 
Sbjct: 301 IDYLNL----------------ASTKQSFGVLP-NSTWNVCSTQPYSAIIRDWFNTPINY 360

Query: 366 VDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTKG 425
           +  LL +   V VYNG  D IC+  G+  W ++LKWK+ + F +  R  L+        G
Sbjct: 361 IPTLL-ENYKVLVYNGNYDWICNFLGSTEWTSQLKWKYNQEFNNSPRKILYINGNT-ISG 384

Query: 426 FTKSHN 428
           +++S++
Sbjct: 421 YSQSYD 384

BLAST of Carg27727 vs. ExPASy TrEMBL
Match: A0A6J1IG20 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474281 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.2e-232
Identity = 398/426 (93.43%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL  CFVLIASSSLFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT AWINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. ExPASy TrEMBL
Match: A0A6J1JCE4 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111483771 PE=3 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.3e-230
Identity = 395/426 (92.72%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           MAKLLVL  CFVLIASSSLFHVG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MAKLLVLHLCFVLIASSSLFHVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMD+LAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDTLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQS+LVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSNLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT+ WINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTQVWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. ExPASy TrEMBL
Match: A0A6J1ID81 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474900 PE=3 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.3e-230
Identity = 396/426 (92.96%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL  CFVLIASSSLF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVDELLAKGVNVTVY+GQVDLICSTKGT AWINKL+W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDELLAKGVNVTVYSGQVDLICSTKGTHAWINKLEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. ExPASy TrEMBL
Match: A0A6J1IKN9 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474883 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.5e-229
Identity = 394/426 (92.49%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M KLLVL  CFVLIASSSLF+VG SITHKSQDG+EEWGYVQVRPKAHMFWWLYRSPSRVD
Sbjct: 1   MVKLLVLHLCFVLIASSSLFYVGTSITHKSQDGSEEWGYVQVRPKAHMFWWLYRSPSRVD 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
           VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWL+KADLLFVDNPVGTGY
Sbjct: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLKKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLR 180
           SYVESGGEFAKGDVE A DMTTLL EVSN+TVGLRN PLYIFAESYGGKFAVTLALSLLR
Sbjct: 121 SYVESGGEFAKGDVETAIDMTTLLVEVSNDTVGLRNAPLYIFAESYGGKFAVTLALSLLR 180

Query: 181 AIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVK 240
           AIQAGNLTLN+GGV+LGNSWMSPEDYTSSWGPLLKDLSRIDD TLQ+SNSLALKIKQQ+K
Sbjct: 181 AIQAGNLTLNLGGVALGNSWMSPEDYTSSWGPLLKDLSRIDDKTLQESNSLALKIKQQIK 240

Query: 241 NRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRK 300
           N LYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTT NSVESNRIDSLEVRK
Sbjct: 241 NTLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTKNSVESNRIDSLEVRK 300

Query: 301 NSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRIN 360
           NSPYLSLN+YKPGA GDLEALMNGPIKQKLTIIP NVTWGGQSDLVFQFFT DFMKPRI 
Sbjct: 301 NSPYLSLNVYKPGATGDLEALMNGPIKQKLTIIPLNVTWGGQSDLVFQFFTGDFMKPRIK 360

Query: 361 EVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTK 420
           EVD LLAKGVNVTVY+GQVDLICSTKGT AWINK +W+ LKAFISKERTTLFCG EKHTK
Sbjct: 361 EVDVLLAKGVNVTVYSGQVDLICSTKGTHAWINKFEWEGLKAFISKERTTLFCGNEKHTK 420

Query: 421 GFTKSH 427
           GFTKSH
Sbjct: 421 GFTKSH 426

BLAST of Carg27727 vs. ExPASy TrEMBL
Match: A0A6J1JE07 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111484161 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.5e-205
Identity = 363/431 (84.22%), Postives = 383/431 (88.86%), Query Frame = 0

Query: 1   MAKLLVLRFCFVLIASSSLFHVGASITHKSQDGTEEWGYVQVRPKAHMFWWLYRSPSRVD 60
           M K  VLR CF LIAS +LFHVG SI  K QDG EEWGYVQVRPKAHMFWWLYRSP RV 
Sbjct: 1   MDKRFVLRLCFTLIASFTLFHVGTSI--KFQDGNEEWGYVQVRPKAHMFWWLYRSPLRVY 60

Query: 61  VGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGY 120
            GSKPWPTILWLQGGPGGSGTGFGNFLEIGPLN NLKPRNSTWLQKADLLFVDNPVGTGY
Sbjct: 61  AGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLNLNLKPRNSTWLQKADLLFVDNPVGTGY 120

Query: 121 SYVESGGEFAKGDVEAATDMTTLLAEVS-NNTVGLRNTPLYIFAESYGGKFAVTLALSLL 180
           SYVESGGEFAKGDVEAA DMTTLLAEVS NNTV LRN  LYIFAESYGGKFA+TLALSLL
Sbjct: 121 SYVESGGEFAKGDVEAAIDMTTLLAEVSKNNTVCLRNAHLYIFAESYGGKFAITLALSLL 180

Query: 181 RAIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQV 240
           +AIQAGNL LN+GGV+LGNSWMSPEDYT SWGPLLKDLSRIDD+TLQ SN LA KIKQQ+
Sbjct: 181 QAIQAGNLALNLGGVALGNSWMSPEDYTFSWGPLLKDLSRIDDVTLQASNRLAWKIKQQI 240

Query: 241 KNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAA----TTTTNSVESNRIDS 300
           KN+LYENAT+SW+DLE LI + SNHVDFYNFMLDFEMD L +    TTTT+SVE NRIDS
Sbjct: 241 KNKLYENATVSWSDLEELIITKSNHVDFYNFMLDFEMDPLTSTTITTTTTSSVEPNRIDS 300

Query: 301 LEVRKNSPYLSLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFM 360
            EV+KNS YLS +IYKPG+ G LEALMNGPIKQKL IIP NV WGGQSDLVFQFFTRDFM
Sbjct: 301 FEVKKNSRYLSSHIYKPGSGGGLEALMNGPIKQKLAIIPPNVKWGGQSDLVFQFFTRDFM 360

Query: 361 KPRINEVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGK 420
           K RINEVDELLAKG+NVT+YNGQVDLICSTKGTEAWINKLKW+ LKAFI+KERT LFCG 
Sbjct: 361 KSRINEVDELLAKGINVTIYNGQVDLICSTKGTEAWINKLKWEGLKAFINKERTPLFCGN 420

Query: 421 EKHTKGFTKSH 427
           EK TKGFTKSH
Sbjct: 421 EKQTKGFTKSH 429

BLAST of Carg27727 vs. TAIR 10
Match: AT2G27920.1 (serine carboxypeptidase-like 51 )

HSP 1 Score: 459.5 bits (1181), Expect = 2.9e-129
Identity = 232/403 (57.57%), Postives = 292/403 (72.46%), Query Frame = 0

Query: 30  SQDGTEEWGYVQVRPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEI 89
           + DG+E WGYV+VRPKAHMFWW Y+SP RV+  SKPWP ILWLQGGPG SG G GNF E+
Sbjct: 29  NSDGSEAWGYVEVRPKAHMFWWHYKSPYRVENPSKPWPIILWLQGGPGASGVGIGNFQEV 88

Query: 90  GPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDVEAATDMTTLLAEV 149
           GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D EAA D+T LL ++
Sbjct: 89  GPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQL 148

Query: 150 SNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGVSLGNSWMSPEDYT 209
            N    L  +PL+I AESYGGK AV L LS++ A+Q+G L L++GGV LG+SW+SPED+ 
Sbjct: 149 FNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFV 208

Query: 210 SSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFSNSNHVDF 269
            SWGPLLK +SR+DD  L  SNSLA KIK Q+KN  Y  AT +W DLE LI S SN VDF
Sbjct: 209 FSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDLENLISSKSNFVDF 268

Query: 270 YNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYL----SLNIYKPGAKGDLEALMN 329
           YNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  +   +GDL+ LMN
Sbjct: 269 YNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDVE-DVEGDLDKLMN 328

Query: 330 GPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQVDLIC 389
           G IK+KL IIP+++ WG  SD VF      FMKP I +VDELLA GV+VT+YNGQ+D+IC
Sbjct: 329 GVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGVDVTIYNGQLDVIC 388

Query: 390 STKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTKGFTKSH 427
           ST GTEAW++KL+W+ L+ F   ER  LFC  ++ T+GFTKS+
Sbjct: 389 STSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSY 425

BLAST of Carg27727 vs. TAIR 10
Match: AT2G27920.2 (serine carboxypeptidase-like 51 )

HSP 1 Score: 375.2 bits (962), Expect = 7.2e-104
Identity = 197/356 (55.34%), Postives = 252/356 (70.79%), Query Frame = 0

Query: 77  GGSGTGFGNFLEIGPLNSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGE--FAKGDV 136
           G SG G GNF E+GPL++ LKPRNSTWL+KADLLFVD+PVG GYS+VE   +  + K D 
Sbjct: 4   GASGVGIGNFQEVGPLDTFLKPRNSTWLKKADLLFVDSPVGAGYSFVEGNQKDLYVKSDE 63

Query: 137 EAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGV 196
           EAA D+T LL ++ N    L  +PL+I AESYGGK AV L LS++ A+Q+G L L++GGV
Sbjct: 64  EAAQDLTKLLQQLFNKNQTLNQSPLFIVAESYGGKIAVKLGLSVIDAVQSGKLKLHLGGV 123

Query: 197 SLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDL 256
            LG+SW+SPED+  SWGPLLK +SR+DD  L  SNSLA KIK Q+KN  Y  AT +W DL
Sbjct: 124 ILGDSWISPEDFVFSWGPLLKHVSRLDDNGLDSSNSLAEKIKTQIKNGEYVGATQTWMDL 183

Query: 257 EGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYL----SLNIY 316
           E LI S SN VDFYNF+LD  MD ++ TT+    +  +I     +K S YL    SL+  
Sbjct: 184 ENLISSKSNFVDFYNFLLDTGMDPVSLTTSLKIKKEEKI-----KKYSRYLNDMRSLSDV 243

Query: 317 KPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGV 376
           +   +GDL+ LMNG IK+KL IIP+++ WG  SD VF      FMKP I +VDELLA GV
Sbjct: 244 E-DVEGDLDKLMNGVIKKKLKIIPNDLIWGNNSDDVFTAMEAAFMKPVIEDVDELLATGV 303

Query: 377 NVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERTTLFCGKEKHTKGFTKSH 427
           +VT+YNGQ+D+ICST GTEAW++KL+W+ L+ F   ER  LFC  ++ T+GFTKS+
Sbjct: 304 DVTIYNGQLDVICSTSGTEAWVHKLRWEGLEEFKKMEREPLFCESDRATRGFTKSY 353

BLAST of Carg27727 vs. TAIR 10
Match: AT2G27920.3 (serine carboxypeptidase-like 51 )

HSP 1 Score: 312.8 bits (800), Expect = 4.4e-85
Identity = 168/317 (53.00%), Postives = 218/317 (68.77%), Query Frame = 0

Query: 116 VGTGYSYVESGGE--FAKGDVEAATDMTTLLAEVSNNTVGLRNTPLYIFAESYGGKFAVT 175
           VG GYS+VE   +  + K D EAA D+T LL ++ N    L  +PL+I AESYGGK AV 
Sbjct: 48  VGAGYSFVEGNQKDLYVKSDEEAAQDLTKLLQQLFNKNQTLNQSPLFIVAESYGGKIAVK 107

Query: 176 LALSLLRAIQAGNLTLNIGGVSLGNSWMSPEDYTSSWGPLLKDLSRIDDMTLQKSNSLAL 235
           L LS++ A+Q+G L L++GGV LG+SW+SPED+  SWGPLLK +SR+DD  L  SNSLA 
Sbjct: 108 LGLSVIDAVQSGKLKLHLGGVILGDSWISPEDFVFSWGPLLKHVSRLDDNGLDSSNSLAE 167

Query: 236 KIKQQVKNRLYENATLSWNDLEGLIFSNSNHVDFYNFMLDFEMDSLAATTTTNSVESNRI 295
           KIK Q+KN  Y  AT +W DLE LI S SN VDFYNF+LD  MD ++ TT+    +  +I
Sbjct: 168 KIKTQIKNGEYVGATQTWMDLENLISSKSNFVDFYNFLLDTGMDPVSLTTSLKIKKEEKI 227

Query: 296 DSLEVRKNSPYL----SLNIYKPGAKGDLEALMNGPIKQKLTIIPSNVTWGGQSDLVFQF 355
                +K S YL    SL+  +   +GDL+ LMNG IK+KL IIP+++ WG  SD VF  
Sbjct: 228 -----KKYSRYLNDMRSLSDVE-DVEGDLDKLMNGVIKKKLKIIPNDLIWGNNSDDVFTA 287

Query: 356 FTRDFMKPRINEVDELLAKGVNVTVYNGQVDLICSTKGTEAWINKLKWKWLKAFISKERT 415
               FMKP I +VDELLA GV+VT+YNGQ+D+ICST GTEAW++KL+W+ L+ F   ER 
Sbjct: 288 MEAAFMKPVIEDVDELLATGVDVTIYNGQLDVICSTSGTEAWVHKLRWEGLEEFKKMERE 347

Query: 416 TLFCGKEKHTKGFTKSH 427
            LFC  ++ T+GFTKS+
Sbjct: 348 PLFCESDRATRGFTKSY 358

BLAST of Carg27727 vs. TAIR 10
Match: AT3G45010.1 (serine carboxypeptidase-like 48 )

HSP 1 Score: 97.1 bits (240), Expect = 3.8e-20
Identity = 88/386 (22.80%), Postives = 158/386 (40.93%), Query Frame = 0

Query: 31  QDGTEEWGYVQV--RPKAHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLE 90
           QD     GY ++     A MF++ + S +     +K  P ++WL GGPG S +    F E
Sbjct: 97  QDFGHHAGYYKLPNSKAARMFYFFFESRT-----NKADPVVIWLTGGPGCS-SELALFYE 156

Query: 91  IGPL----NSNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDMTTLL 150
            GP     NS+L      W + ++L++VD PVGTG+SY     +    +   + D+   L
Sbjct: 157 NGPFTVSNNSSLSWNEFGWDKASNLIYVDQPVGTGFSYTSDQSDLRHDEDGVSNDLYDFL 216

Query: 151 AEVSNNTVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLT-LNIGGVSLGNSWMSP 210
                          YI  ESY G +   LA  + R  +    T +N+ G ++GN   +P
Sbjct: 217 QAFFKEHPQFVKNDFYITGESYAGHYIPALASRVHRGNKNKEGTHINLKGFAIGNGLTNP 276

Query: 211 EDYTSSWGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFSNSN 270
           E    ++     D++ I        N      +Q +K    +                  
Sbjct: 277 EIQYGAYADYALDMNLITQSDHDNLNRYYATCQQSIKECSADGG---------------- 336

Query: 271 HVDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYLSLNIYKPGAK----GDLE 330
                      E D+ A++ T  +    +I  +++  N  Y  +     G+      ++E
Sbjct: 337 -----------EGDACASSYTVCNNIFQKI--MDIAGNVNYYDVRKQCEGSLCYDFSNME 396

Query: 331 ALMNGPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQV 390
             +N    +K   +  ++ +   S  V++    D+M+     +  LL  G+ + VY G+ 
Sbjct: 397 NFLNQKSVRKALGV-GDIEFVSCSTAVYEAMQMDWMRNLEVGIPALLQDGIKLLVYAGEY 446

Query: 391 DLICSTKGTEAWINKLKWKWLKAFIS 406
           DLIC+  G   W+++++W   K F++
Sbjct: 457 DLICNWLGNSKWVHEMEWSGQKEFVA 446

BLAST of Carg27727 vs. TAIR 10
Match: AT1G15000.1 (serine carboxypeptidase-like 50 )

HSP 1 Score: 96.3 bits (238), Expect = 6.5e-20
Identity = 93/385 (24.16%), Postives = 156/385 (40.52%), Query Frame = 0

Query: 38  GYVQVRPK--AHMFWWLYRSPSRVDVGSKPWPTILWLQGGPGGSGTGFGNFLEIGPLN-- 97
           GY+ V+P   + MF+  Y +           P ++WLQGGPG S    GNF E+GP    
Sbjct: 38  GYLPVKPAPGSSMFYAFYEA-QEPTTPLPDTPLLVWLQGGPGCSSM-IGNFYELGPWRVV 97

Query: 98  ---SNLKPRNSTWLQKADLLFVDNPVGTGYSYVESGGEFAKGDVEAATDMTTLLAEVSNN 157
              ++L+     W +   LLFVDNP+G G+S   S  +      + A  +   L E    
Sbjct: 98  SRATDLERNPGAWNRLFGLLFVDNPIGVGFSIAASQQDIPTNQRQVAEHLYAALVEFLEQ 157

Query: 158 TVGLRNTPLYIFAESYGGKFAVTLALSLLRAIQAGNLTLNIGGVSLGNSWMSPEDYTSS- 217
                N P+Y   ESY GK+   +   +L+  +  N  +N+ G+++GN    P     + 
Sbjct: 158 NPSFENRPVYFTGESYAGKYVPAIGYYILK--EKPNGKVNLKGLAIGNGLTDPVTQVQTH 217

Query: 218 -----WGPLLKDLSRIDDMTLQKSNSLALKIKQQVKNRLYENATLSWNDLEGLIFSNSNH 277
                +  L+    R++   LQK+  +++ +   VK++ +  A  +  +L  L+ + +  
Sbjct: 218 AVNVYYSGLVNAKQRVE---LQKAQEISVAL---VKSQKWREAADARTELLTLLSNMTGL 277

Query: 278 VDFYNFMLDFEMDSLAATTTTNSVESNRIDSLEVRKNSPYLSLNIYKPGAKGDLEA-LMN 337
              YN              T  ++              PY          + DL   L+N
Sbjct: 278 ATLYN--------------TARAI--------------PY----------RTDLVVDLLN 337

Query: 338 GPIKQKLTIIPSNVTWGGQSDLVFQFFTRDFMKPRINEVDELLAKGVNVTVYNGQVDLIC 397
               +++  +   V +   SD V      D MK  +  + E   +   V +Y G +DL  
Sbjct: 338 QREAKRVLGVSETVRFEECSDEVEDVLRADVMK-SVKFMVEYALERTQVLLYQGMLDLRD 373

Query: 398 STKGTEAWINKLKWKWLKAFISKER 409
               TE W+  + W  L  F + ER
Sbjct: 398 GVVSTEEWMKTMNWSGLGMFSTAER 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029856.11.8e-250100.00Serine carboxypeptidase-like 51, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_023515401.12.3e-23795.54serine carboxypeptidase-like 51 [Cucurbita pepo subsp. pepo][more]
XP_022975190.11.9e-23193.43serine carboxypeptidase-like 51 [Cucurbita maxima][more]
XP_022985845.12.7e-23092.72serine carboxypeptidase-like 51 [Cucurbita maxima][more]
XP_022975497.12.7e-23092.96serine carboxypeptidase-like 51 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q67Y834.1e-12857.57Serine carboxypeptidase-like 51 OS=Arabidopsis thaliana OX=3702 GN=SCPL51 PE=2 S... [more]
Q920A51.4e-9143.43Retinoid-inducible serine carboxypeptidase OS=Mus musculus OX=10090 GN=Scpep1 PE... [more]
Q9HB401.8e-9145.83Retinoid-inducible serine carboxypeptidase OS=Homo sapiens OX=9606 GN=SCPEP1 PE=... [more]
Q920A68.9e-9144.37Retinoid-inducible serine carboxypeptidase OS=Rattus norvegicus OX=10116 GN=Scpe... [more]
Q54DY71.6e-2624.18Serine carboxypeptidase S10 family member 1 OS=Dictyostelium discoideum OX=44689... [more]
Match NameE-valueIdentityDescription
A0A6J1IG209.2e-23293.43Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474281 PE=3 SV=1[more]
A0A6J1JCE41.3e-23092.72Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111483771 PE=3 SV=1[more]
A0A6J1ID811.3e-23092.96Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474900 PE=3 SV=1[more]
A0A6J1IKN92.5e-22992.49Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111474883 PE=3 SV=1[more]
A0A6J1JE072.5e-20584.22Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111484161 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G27920.12.9e-12957.57serine carboxypeptidase-like 51 [more]
AT2G27920.27.2e-10455.34serine carboxypeptidase-like 51 [more]
AT2G27920.34.4e-8553.00serine carboxypeptidase-like 51 [more]
AT3G45010.13.8e-2022.80serine carboxypeptidase-like 48 [more]
AT1G15000.16.5e-2024.16serine carboxypeptidase-like 50 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 14..432
e-value: 1.1E-78
score: 267.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 27..427
IPR001563Peptidase S10, serine carboxypeptidasePFAMPF00450Peptidase_S10coord: 32..426
e-value: 1.1E-62
score: 212.9
IPR001563Peptidase S10, serine carboxypeptidasePANTHERPTHR11802SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASEcoord: 16..420
NoneNo IPR availablePANTHERPTHR11802:SF345CARBOXYPEPTIDASEcoord: 16..420
IPR018202Serine carboxypeptidase, serine active sitePROSITEPS00131CARBOXYPEPT_SER_SERcoord: 161..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27727-RACarg27727-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004185 serine-type carboxypeptidase activity