Carg27694 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27694
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding protein CML36
Location	Carg_Chr03: 2375070 .. 2375621 (+)
RNA-Seq Expression	Carg27694
Synteny	Carg27694

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGAAGTCTCCCGTTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCTTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCCGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGGACGTGATGCTTTTGATTGGACGGCTGTGA

mRNA sequence

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGAAGTCTCCCGTTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCTTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCCGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGGACGTGATGCTTTTGATTGGACGGCTGTGA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGAAGTCTCCCGTTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCTTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCCGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGGACGTGATGCTTTTGATTGGACGGCTGTGA

Protein sequence

MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDVMLLIGRL

Homology

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BLAST of Carg27694 vs. NCBI nr
Match: KAG7033603.1 (putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 351.3 bits (900), Expect = 5.1e-93
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDVMLLI 180
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDVMLLI
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDVMLLI 180

Query: 181 GRL 184
           GRL
Sbjct: 181 GRL 183

[Close]

BLAST of Carg27694 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.4 bits (864), Expect = 7.6e-89
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 175

[Close]

BLAST of Carg27694 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.8 bits (852), Expect = 1.9e-87
Identity = 172/175 (98.29%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           D+V ESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 175

[Close]

BLAST of Carg27694 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 323.6 bits (828), Expect = 1.1e-84
Identity = 171/175 (97.71%), Postives = 172/175 (98.29%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 174

[Close]

BLAST of Carg27694 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 270.0 bits (689), Expect = 1.5e-68
Identity = 151/179 (84.36%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARV
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           GSG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDEN 174

[Close]

BLAST of Carg27694 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-30
Identity = 97/188 (51.60%), Postives = 118/188 (62.77%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSS--SSENFFKPHTAGYGTPTSVLLAG 65
           R SPKRLFR+K    A VSRS+P SF S  SSSSS  S  N  +  TA   TP SVL   
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLPQN 68

Query: 66  CSD----------SDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDG--GGFI 125
             D             R++DGVVSR +L A++SR++    S+EEV++ML EVDG  GG I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 126 RLDELMARVG--SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDC 174
            L++L +RV   SG  +V   ELRE FE FD D +G+ISAEELH V+  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

[Close]

BLAST of Carg27694 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-29
Identity = 91/192 (47.40%), Postives = 114/192 (59.38%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MK+ K   PKR F   K+ S         SFGS +SSS    +N       G  TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  -----------LAGCSDSDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEV--DG 120
                      +        R+NDG VSR +LE++LSR+     +EEE+ +ML EV  DG
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 GGFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLE 176
            G IRL+EL +RV S   A   +EL+ETFEFFDAD DG ISA+EL  V+ +IGDERCTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

[Close]

BLAST of Carg27694 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.8e-15
Identity = 49/114 (42.98%), Postives = 71/114 (62.28%), Query Frame = 0


Query: 70  RENDGVVSRKELEAI---LSRIASEEEVAMMLSE--VDGGGFIRLDELMARVGSGFDAVG 129
           + NDG +S  EL+ +   LS  AS+EE   M+ E  +DG GFI LDE +A       +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 130 ES---ELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
            S   +L+E F+ +D D +GRISA ELH V +++G E+C+++DC+RMI  VD D
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSD 137

[Close]

BLAST of Carg27694 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 49/116 (42.24%), Postives = 71/116 (61.21%), Query Frame = 0


Query: 70  RENDGVVSRKELEAI---LSRIASEEEVAMMLS--EVDGGGFIRLDELMARVGSGFDAVG 129
           +  DG +S  EL+ +   LS  AS EE   M+   ++DG GFI LDE +A    G    G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 130 E-----SELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
                 S+L+E FE +D D +GRISA+ELH V +++G E+C+++DC++MI  VD D
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDID 141

[Close]

BLAST of Carg27694 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.9e-13
Identity = 50/110 (45.45%), Postives = 66/110 (60.00%), Query Frame = 0


Query: 73  DGVVSRKELEAI---LSRIASEEEVAMMLSE--VDGGGFIRLDELMA--RVGSGFDAVGE 132
           DG +SR EL A+   L   A+++E+A M++E   DG GFI LDE  A     SG  A  E
Sbjct: 53  DGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVE 112

Query: 133 SELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
            +LR  F  FDAD +G ISA EL  V   +G E+ T++ CRRMI  VD++
Sbjct: 113 EDLRHAFRVFDADGNGTISAAELARVLHGLG-EKATVQQCRRMIEGVDQN 161

[Close]

BLAST of Carg27694 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 3.7e-89
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 175

[Close]

BLAST of Carg27694 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.5e-85
Identity = 171/175 (97.71%), Postives = 172/175 (98.29%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60

Query: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 174

[Close]

BLAST of Carg27694 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 7.2e-69
Identity = 151/179 (84.36%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARV
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           GSG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDEN 174

[Close]

BLAST of Carg27694 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 7.2e-69
Identity = 151/179 (84.36%), Postives = 163/179 (91.06%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARV
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           GSG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDEN 174

[Close]

BLAST of Carg27694 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.2e-66
Identity = 149/179 (83.24%), Postives = 159/179 (88.83%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61

Query: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120
           VL     DSD R N+GVVSRKELEAILSRIASEEEVAMML EVD G  GFIRL+ELMARV
Sbjct: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARV 121

Query: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
           GSG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+
Sbjct: 122 GSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDEN 174

[Close]

BLAST of Carg27694 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 9.1e-32
Identity = 97/188 (51.60%), Postives = 118/188 (62.77%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSS--SSENFFKPHTAGYGTPTSVLLAG 65
           R SPKRLFR+K    A VSRS+P SF S  SSSSS  S  N  +  TA   TP SVL   
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLPQN 68

Query: 66  CSD----------SDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDG--GGFI 125
             D             R++DGVVSR +L A++SR++    S+EEV++ML EVDG  GG I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 126 RLDELMARVG--SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDC 174
            L++L +RV   SG  +V   ELRE FE FD D +G+ISAEELH V+  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

[Close]

BLAST of Carg27694 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-30
Identity = 91/192 (47.40%), Postives = 114/192 (59.38%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MK+ K   PKR F   K+ S         SFGS +SSS    +N       G  TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  -----------LAGCSDSDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEV--DG 120
                      +        R+NDG VSR +LE++LSR+     +EEE+ +ML EV  DG
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 GGFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLE 176
            G IRL+EL +RV S   A   +EL+ETFEFFDAD DG ISA+EL  V+ +IGDERCTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

[Close]

BLAST of Carg27694 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 82.4 bits (202), Expect = 4.1e-16
Identity = 49/114 (42.98%), Postives = 71/114 (62.28%), Query Frame = 0


Query: 70  RENDGVVSRKELEAI---LSRIASEEEVAMMLSE--VDGGGFIRLDELMARVGSGFDAVG 129
           + NDG +S  EL+ +   LS  AS+EE   M+ E  +DG GFI LDE +A       +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 130 ES---ELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
            S   +L+E F+ +D D +GRISA ELH V +++G E+C+++DC+RMI  VD D
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSD 137

[Close]

BLAST of Carg27694 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-16
Identity = 49/116 (42.24%), Postives = 71/116 (61.21%), Query Frame = 0


Query: 70  RENDGVVSRKELEAI---LSRIASEEEVAMMLS--EVDGGGFIRLDELMARVGSGFDAVG 129
           +  DG +S  EL+ +   LS  AS EE   M+   ++DG GFI LDE +A    G    G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 130 E-----SELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
                 S+L+E FE +D D +GRISA+ELH V +++G E+C+++DC++MI  VD D
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDID 141

[Close]

BLAST of Carg27694 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 75.5 bits (184), Expect = 5.0e-14
Identity = 46/109 (42.20%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 73  DGVVSRKELEAILSRI---ASEEEVAMMLSEVD--GGGFIRLDELMARVGSGFDAVGESE 132
           DG +S KEL AI++ +     EEE+   ++E+D  G G+I  +E +     G D     E
Sbjct: 50  DGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLE 109

Query: 133 -LRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 176
            L++ F  +D D +G ISAEELH V RS+GDE C++ +CR+MI  VD+D
Sbjct: 110 NLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKD 157

The following BLAST results are available for this feature:

BLAST of Carg27694 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033603.1	5.1e-93	100.00	putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022932888.1	7.6e-89	100.00	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_023543601.1	1.9e-87	98.29	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_022967907.1	1.1e-84	97.71	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_008457589.1	1.5e-68	84.36	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]

BLAST of Carg27694 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30188	1.3e-30	51.60	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9SS31	1.8e-29	47.40	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
Q9C8Y1	5.8e-15	42.98	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	1.3e-14	42.24	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q8RZB5	1.9e-13	45.45	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg27694 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F310	3.7e-89	100.00	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	5.5e-85	97.71	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A5A7VA05	7.2e-69	84.36	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	7.2e-69	84.36	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]
A0A0A0L0H1	1.2e-66	83.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]

BLAST of Carg27694 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41410.1	9.1e-32	51.60	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	1.3e-30	47.40	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	4.1e-16	42.98	calmodulin like 23	[more]
AT5G37770.1	9.1e-16	42.24	EF hand calcium-binding protein family	[more]
AT1G24620.1	5.0e-14	42.20	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 127..155 e-value: 4.5E-5 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 128..148 e-value: 3.2E-7 score: 29.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 123..158 score: 14.42972
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 127..175 e-value: 6.66265E-10 score: 51.0093
None	No IPR available	GENE3D	1.10.238.10		coord: 66..176 e-value: 7.9E-17 score: 63.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..175
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..175
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 136..148
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 71..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27694-RA	Carg27694-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding