Carg27650 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg27650 vs. NCBI nr
Match: KAG7012765.1 (D-cysteine desulfhydrase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK
Sbjct: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK
Sbjct: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH
Sbjct: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG
Sbjct: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360

Query: 361 TTAIGLGLGALCLGCGH 378
           TTAIGLGLGALCLGCGH
Sbjct: 361 TTAIGLGLGALCLGCGH 377

BLAST of Carg27650 vs. NCBI nr
Match: XP_022944821.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 2.4e-190
Identity = 343/374 (91.71%), Postives = 353/374 (94.39%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASARSVGSFTSLP RPRD SS FR RRTVVQSRMGGSGFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTEDTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNS+PVDYDFH+RARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKV+VINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVVVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 360

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 362

BLAST of Carg27650 vs. NCBI nr
Match: XP_023541195.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.2 bits (1733), Expect = 2.7e-189
Identity = 342/374 (91.44%), Postives = 350/374 (93.58%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMAS RSVGSFTSLPNRPRDVSS FRSRRTVVQSRMGG GFFFRATKCRLLHSK
Sbjct: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR WALANP+SKINKVIL T DTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNSVPVDYDFH+RARKH
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKVIVINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFV+DAGTG
Sbjct: 301 ERKVIVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVIDAGTG 360

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 362

BLAST of Carg27650 vs. NCBI nr
Match: XP_022944822.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 660.6 bits (1703), Expect = 8.1e-186
Identity = 338/374 (90.37%), Postives = 348/374 (93.05%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASARSVGSFTSLP RPRD SS FR RRTVVQSRMGGSGFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTEDTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNS+PVDYDFH+RARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKV+VINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVVVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 357

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 357

BLAST of Carg27650 vs. NCBI nr
Match: XP_023541196.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.1 bits (1694), Expect = 8.9e-185
Identity = 337/374 (90.11%), Postives = 345/374 (92.25%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMAS RSVGSFTSLPNRPRDVSS FRSRRTVVQSRMGG GFFFRATKCRLLHSK
Sbjct: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR WALANP+SKINKVIL T DTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNSVPVDYDFH+RARKH
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKVIVINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFV+DAGTG
Sbjct: 301 ERKVIVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVIDAGTG 357

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 357

BLAST of Carg27650 vs. ExPASy Swiss-Prot
Match: A1L4V7 (D-cysteine desulfhydrase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g26115 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.3e-77
Identity = 160/304 (52.63%), Postives = 207/304 (68.09%), Query Frame = 0

Query: 71  SSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLKDSPANHSCLA 130
           SS+++  +S++      +FV +LLDR+W L  P S I ++ +S+     K S  N++   
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGIDKFSFLNNTRPH 82

Query: 131 VDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
           +        D    +    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 FKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKHERKVIVINEG 310
            +T A+LVAG  G++LW  DI +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLGLGA 370
           AGD++ALL            G+ RLV +LSQDHL GK+R +KFVVDAGTGTTA+GLG+ A
Sbjct: 263 AGDALALL------------GMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAA 296

Query: 371 LCLG 375
           + LG
Sbjct: 323 MSLG 296

BLAST of Carg27650 vs. ExPASy Swiss-Prot
Match: B9EYZ1 (Putative D-cysteine desulfhydrase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0695600 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 9.3e-60
Identity = 134/291 (46.05%), Postives = 169/291 (58.08%), Query Frame = 0

Query: 85  GGEDFVVRLLD-REWALANPDSKINKVILSTEDTNLKDSPANHSCLAVDTNICTYNDLAR 144
           GG   V  LL   EW L +P ++++ +  S   ++   SP +H   +  T     N    
Sbjct: 9   GGGRTVANLLSATEWMLPSPATQVHTI--SVLPSHSPPSPPHHFAFSNLTTAPKRNGGKG 68

Query: 145 ANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVL 204
                  F VVRDDLLHPL NGNKARKLD  LPL+     TDVVTCGGCQSAHAAATAV 
Sbjct: 69  EEEGRPRFEVVRDDLLHPLANGNKARKLDALLPLLRRRGATDVVTCGGCQSAHAAATAVH 128

Query: 205 CAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMFKTQAELVAGSSG 264
           CAE G+R H+LLRGEQP++ TGYNL+S ++GNV Y  RS+YA+R++M    A  VAG+ G
Sbjct: 129 CAEWGMRPHILLRGEQPDIPTGYNLISLMFGNVAYASRSVYAHRDEMLYNHARKVAGTGG 188

Query: 265 SILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKHERKVIVINEGAGDSIALLGILSL 324
           ++LW DDI +           N   +            R+V++I EGAGD          
Sbjct: 189 TVLWADDISKEDFV---LDEDNGCEIG----------SRRVVIIKEGAGD---------- 248

Query: 325 LKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLGLGALCLG 375
             V A +G+IRLV YL     F K   +  VVDAGTGTTA+GL LGA+CLG
Sbjct: 249 --VQALLGVIRLVEYLYNLSSFHKHENVHVVVDAGTGTTAVGLALGAVCLG 272

BLAST of Carg27650 vs. ExPASy Swiss-Prot
Match: Q9WY68 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0225 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.0e-07
Identity = 65/221 (29.41%), Postives = 87/221 (39.37%), Query Frame = 0

Query: 150 SFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGL 209
           + YV RDDL   + +GNK RKL+  L        T V TCGG QS HA ATA +    GL
Sbjct: 25  NIYVKRDDLTELVGSGNKIRKLEYLLWEALKKGATTVFTCGGLQSNHARATAYVSRRYGL 84

Query: 210 RSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMFKTQAELVAGSSGSILWFD 269
           +  L LR +  ++L G  L+  + G                    AE+V  S       D
Sbjct: 85  KPVLFLR-KGEKVLNGNLLLDILLG--------------------AEIVEVSPEEYERID 144

Query: 270 DIFQSSITKQPCSTTNSVPVDYDFHLRAR-KHERKVIVINEGAGDSIALLGILSLLKVHA 329
           +IF                   D H + R K   KV VI EG  +S+   G       +A
Sbjct: 145 EIF-------------------DVHKKMREKKGEKVYVIPEGGSNSLGAFGYF-----NA 191

Query: 330 FVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLGLG 370
            + +   +N  S D +         V   G+G T  GL  G
Sbjct: 205 VLEMKDQLNLESFDAI---------VCAVGSGGTIAGLSAG 191

BLAST of Carg27650 vs. ExPASy TrEMBL
Match: A0A6J1FZ47 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 343/374 (91.71%), Postives = 353/374 (94.39%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASARSVGSFTSLP RPRD SS FR RRTVVQSRMGGSGFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTEDTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNS+PVDYDFH+RARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKV+VINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVVVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 360

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 362

BLAST of Carg27650 vs. ExPASy TrEMBL
Match: A0A6J1FZ73 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 3.9e-186
Identity = 338/374 (90.37%), Postives = 348/374 (93.05%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASARSVGSFTSLP RPRD SS FR RRTVVQSRMGGSGFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TATSAMDNEYSSRKLT+SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTEDTNL+
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+RANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM KTQAELVAGSSGSILWFDD+FQSSI KQPCSTTNS+PVDYDFH+RARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKV+VINEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVVVINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 357

Query: 361 TTAIGLGLGALCLG 375
           TTAIGLGLGALCLG
Sbjct: 361 TTAIGLGLGALCLG 357

BLAST of Carg27650 vs. ExPASy TrEMBL
Match: A0A6J1HST3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 334/373 (89.54%), Postives = 344/373 (92.23%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASA SVGSFTS PN PR+VSS FR RRTVVQSR+GGSGFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TA  AMDNEY SRKLT SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTE T++K
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLAR NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM +T+AELVAGSSGSILWFDDIFQSSI KQPCSTTNSV VDYDFH+RARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKVI+INEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVIIINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 360

Query: 361 TTAIGLGLGALCL 374
           TTAIGLGLGALCL
Sbjct: 361 TTAIGLGLGALCL 361

BLAST of Carg27650 vs. ExPASy TrEMBL
Match: A0A6J1HUH2 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.1e-178
Identity = 329/373 (88.20%), Postives = 339/373 (90.88%), Query Frame = 0

Query: 1   MRQAVPKMASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSK 60
           MRQA+PKMASA SVGSFTS PN PR+VSS FR RRTVVQSR+GGSGFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLK 120
           TA  AMDNEY SRKLT SSQVVQFGGEDFV RLLDR+WALANPDSKINKVILSTE T++K
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLAR NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKH 300
           RSIYANREKM +T+AELVAGSSGSILWFDDIFQSSI KQPCSTTNSV VDYDFH+RARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTG 360
           ERKVI+INEGAGDSIALL            GLIRLVNYLSQDHL GKQRAIKFVVDAGTG
Sbjct: 301 ERKVIIINEGAGDSIALL------------GLIRLVNYLSQDHLLGKQRAIKFVVDAGTG 356

Query: 361 TTAIGLGLGALCL 374
           TTAIGLGLGALCL
Sbjct: 361 TTAIGLGLGALCL 356

BLAST of Carg27650 vs. ExPASy TrEMBL
Match: A0A6J1DFQ3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019685 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.6e-150
Identity = 277/367 (75.48%), Postives = 309/367 (84.20%), Query Frame = 0

Query: 8   MASARSVGSFTSLPNRPRDVSSGFRSRRTVVQSRMGGSGFFFRATKCRLLHSKTATSAMD 67
           M SARSV SF S+P+RP DVS   R  RTVV+S MGG G+FF ATK RLLHSKTA +A+D
Sbjct: 1   MGSARSVCSFMSVPSRPGDVSGVLRWWRTVVESEMGGPGYFFSATKLRLLHSKTAIAAVD 60

Query: 68  NEYSSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLKDSPANHS 127
           NE+S R  T+ SQ V+ GGE+FV RLLDR W LA+PD+KI+KV  S  DTNL++SPA+H 
Sbjct: 61  NEFSFRNFTDGSQGVKLGGEEFVSRLLDRRWTLADPDTKISKVTFSNADTNLENSPASHL 120

Query: 128 CLAVDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVV 187
           CLAVDTN CT+ND++R +  EQSFYVVRDDLLHPLINGNKARKLDG LPLIEDN VTDVV
Sbjct: 121 CLAVDTNSCTFNDVSRTDYQEQSFYVVRDDLLHPLINGNKARKLDGVLPLIEDNSVTDVV 180

Query: 188 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANR 247
           TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNL+STIYGNVTYVPR+IYANR
Sbjct: 181 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLISTIYGNVTYVPRAIYANR 240

Query: 248 EKMFKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKHERKVIVI 307
           EKM K+QA+LVAGSS  I+WFDDIF SS+ KQ CS TNSV +DYDF+ RARKH RKVIVI
Sbjct: 241 EKMLKSQADLVAGSSDGIIWFDDIFPSSLKKQACS-TNSVQIDYDFNRRARKHGRKVIVI 300

Query: 308 NEGAGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLG 367
           NEGAGD++ALL            GLIRLV YLSQDHL GKQRA+KFVVDAGTGTTA+GLG
Sbjct: 301 NEGAGDAVALL------------GLIRLVKYLSQDHLLGKQRAVKFVVDAGTGTTAVGLG 354

Query: 368 LGALCLG 375
           LGALCLG
Sbjct: 361 LGALCLG 354

BLAST of Carg27650 vs. TAIR 10
Match: AT3G26115.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 291.6 bits (745), Expect = 9.2e-79
Identity = 160/304 (52.63%), Postives = 207/304 (68.09%), Query Frame = 0

Query: 71  SSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLKDSPANHSCLA 130
           SS+++  +S++      +FV +LLDR+W L  P S I ++ +S+     K S  N++   
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGIDKFSFLNNTRPH 82

Query: 131 VDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
           +        D    +    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 FKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKHERKVIVINEG 310
            +T A+LVAG  G++LW  DI +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLGLGA 370
           AGD++ALL            G+ RLV +LSQDHL GK+R +KFVVDAGTGTTA+GLG+ A
Sbjct: 263 AGDALALL------------GMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAA 296

Query: 371 LCLG 375
           + LG
Sbjct: 323 MSLG 296

BLAST of Carg27650 vs. TAIR 10
Match: AT3G26115.2 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 291.6 bits (745), Expect = 9.2e-79
Identity = 160/304 (52.63%), Postives = 207/304 (68.09%), Query Frame = 0

Query: 71  SSRKLTNSSQVVQFGGEDFVVRLLDREWALANPDSKINKVILSTEDTNLKDSPANHSCLA 130
           SS+++  +S++      +FV +LLDR+W L  P S I ++ +S+     K S  N++   
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGIDKFSFLNNTRPH 82

Query: 131 VDTNICTYNDLARANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
           +        D    +    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 FKTQAELVAGSSGSILWFDDIFQSSITKQPCSTTNSVPVDYDFHLRARKHERKVIVINEG 310
            +T A+LVAG  G++LW  DI +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGILSLLKVHAFVGLIRLVNYLSQDHLFGKQRAIKFVVDAGTGTTAIGLGLGA 370
           AGD++ALL            G+ RLV +LSQDHL GK+R +KFVVDAGTGTTA+GLG+ A
Sbjct: 263 AGDALALL------------GMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAA 296

Query: 371 LCLG 375
           + LG
Sbjct: 323 MSLG 296