Carg27605 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27605
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Amidase 1-like
Location	Carg_Chr10: 7499375 .. 7505934 (+)
RNA-Seq Expression	Carg27605
Synteny	Carg27605

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATAATTTTTGGGCATTTCAGTAACCATTTTTTTAATTCAGCTCTCTTCCTCTTTCATTGTCCTGGTCTTCTCTGCAACTTCCAGCCATTATTAGGGTTTTCGGGGTGGATTTGGATCAGGGAGGCCGATCTCAGGTCTTGCAATGGCTGTGCAGGATCATGGAGCTTTCATAGAGAAATTCATCCTGCAACAGAGCTCCCCGTCCGATCCACTTCCCTTGACTGGCCTTACTTTCGCTGTTAAAGACATGTAAGTTTTCGATTCTTTACTCTTTGGTGATTAAAAGGTTGTGAAATGTGAAATATCTGTATGAGTATTCATGAAAGAATCAAAAGATTTCATGAAGGTTTTGGTGATGACAGGTTTTGAAATCTTGTAGATATTGGTTTTAGATCGAAACTGTTTGTTAGTGTGTGCAAGTCGAAGCTTAGTTCGATAAAATAATTCTTGGTTCTGTGTTTATATTAGTATCTTGGTCCTTGAATTATCAACGTCACAGTTTTTCTACAATGAATTTGGGGTATTATAGTCAAACTTCTCACATTGGGATTTGAACCAAACAGATTTGATATGGAGGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACAGCACCAGCCGTGTTGGCTATCCTAAGAGGAGGAGCCACTTGCATCGGCAAGACTATCATGGATGAAATGGCCTTCAGGTAACAAGAAAACGTACCCTTTACCTTTTTTCCCGTTAGCTTTCATTTTTTTCAATTGATAACAAACAAACAATCCATAAGCTTTGCCACTTGGGATACAGGGGATAATAATTACTCTTTTGAGCTGCACGTCAATTCTATAATTGTGTTAACATAGTGGGTAATAAACTTGACTGAGTAGAGTCAGTTACTAACTGAAGAGAATTATTAGTAAAAATAAGAGATTTGGTAGTAGTTGACCAATTGTGGACAATTTGTGATTGTGGACCAAGTTTTATGCTTTTATTTGATTAAGAAGATTGTTAGTGGTTCTTGACGAGAAAGTTTAGGAAGCTAGTTAGTGTTTTCTCCCCTATAAATAGATACATGACAACTACAACAACAAACAGAATTGAAAACCTAAACAGACTTAATGTTTCTTTGTTCCAAAAAATCTCACCTCTCAAATTTTTTTTTTTTGCTTCTTTTATTTGTTTTGTCATATTGGGATCGTGGGTCAATTCCAACAAAGACTTAATTCTTATCATCTTTTTCAATCAGACAGCTCTGCATTAAAATTGGTTAAAACATGAACGTGAACCAATTGATTTTTATCAGGTTTCTAAATGGTAATTGAAATGCTTTGACCAAATCATTCATCCGAACACGCAGGCTTTTGAGTGAGTCTTTATGTTAGTGGAATCACTAATTTGATAATCATATTTTGTCAGTATAAGTGGGGAAAACTTTCATTATGGCACACCCCGAAACCCATATGCATCAGATCGTATACCTGGAGGATCTTCCAGCGGCTCTGCTGTTGCTGTTGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCTTAGTTCCAAGACGTTGAATAATTTCTGATTCCATGCCTTGTACTGGGTAAAATATTGTAAAAGACCTTACCTAGATTTGTAGGAACCGATACTGGAGGTAGCGTAAGAGTGCCTGCTTCTTATTGTGGAATTCTTGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCCATGACGCAGAGCTTCGATACAGTAGGTAATGTGCAATGTCTCATTGTCACTTTGTCCACCTCTTCTTTCGTAGATTTTTTTTTTCCTACTAAGGCCCTTTGGATATTGTCATGCAAAGTTGTTATTGTTTCCTTTTTCTCTTCACTTATTACTTTTAAATTTTATAAGTAAAAGGTCCTTTGACTAATATTTGTGAAAATCCAATACTGCATAATTTGTTGAAGATTGTTGGGAGAGGAGTCCCATATCGGTTAATTAAGGAATTGATTATGGGTTTATAAATAAGGAATACATATTCATTGGTACGAGACCTTTTGAGGAAACCAAAAGCAAAACCATGAGAGCATAGGTTCAAAGTGAATAATATCATACCATTCTGGAGAGTCATGGTCATGGTTTCTAGCATGGTATCAGAGTCAATCTCTTAACTTAGCCATGTCAATAGAATCCTCAAATGTCGAACAAAGAAGTTGTGAGCCTCGAAGGTGTAGTAAAAAAGTGACTCAAGTGTCGAACAAAGGGTGTTTTTTGTTTGAGGGCTCCAAAGGACTCCAAAGAAGGAGTCAAGCCTCGATAAAGGAGAGGCTATTCGAGGGCTCCATAAGCCTCAGGGGAGACTCTATGGTGTACTTTGTTCCAGAGGAGGATTGTTGAGAGAGGAGTCCCACATCGGCTAATCAAGGAGTTGATTAGGGTTTATAAGCAAGGAATCATCTTCATTGGTATGAGGCCTTTTGAGAAACTAAAAGCAAAACCATGAGAGCTTATGCTCAAAGTGGACAATATCATACTATGGTGGAGAGTTGTGATTTCTAACATCATTGTTATGATGATGGCCATGTTTCTCATGATTTTGCATATAGATGATCAATATGATGTTGATTGGTAGCAGAGCCATTGGTTGCTTATAAATAGAATAGAGCTCATAGCTAAACAAATATTATCTATTGGAACATGTCATCTCTTTCCTTCAACATTAACCATGTTCCTGCCATGTTAAAAAGGTACATTTCTTTGACACCATCTTCTCATTTTAGGTTGGTTTGCCATCGATTCTGCCGTATTCAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGGTAAGCCTGACACCGTTATTTTGTTTTTTTGATTAGTACCATGCTTTCATTGAGGATGAATGAAAAAAATACATGGGCATACAAAAAAAAAATAGTTCAGAAAGTGAGGAGCCAAGCTAAACTAAAAAGGGACTCCAATCAAGCATAATAAGGCCTAAAGTGTAATTATGAAAGTTTTTGAACACCAAGCCCAAAGAGAGACATTAAACCTAACAAGGGGCTAATTCTCCTAGAGAACTCATTAACCCATTAGCATTCTATTGTTTCTCTCAAGCCAAAGGCTTCATAAAACTGCACAACAATGGGGCAATGAGTATCAGTTATTGCAAAGGTTATGGTCTTGAGGAATGGATATTTGATATTGGTTAGTGAATGAATCAGGACTTGATTTTCTTCTCATTCTTTCAGAGAGAACTCATTTGCGATCTGGCTTCTTATTTGAAATATTTGGACTGTAGCCTTTTTGGCACATTCAACAAAAGGTCGATTTAGAGCCAAAGGTTTGCAAGCGGCTAAGAAACACTTCTTTATTGAATTAACAAAACCCTTTGTTAGTTATTTATCTATGAGTTATCTTTGTAACCAAATATAGTAGGGTTAGATTTATTAAAAAATGACGTGTCTGTGAGTCAATAAGCTTGACTTGTACATGATTTATTCTTTGCACGATCTATTAATAGCAGAGTATTTATAGGTGAAGTTATTAATCATGGAGATGGAGCATTTGTAGGAGAAGTTATTAGTAGTGGAACATTTCTAGAAATATTAGTCCTGGAACAATTTTAGGAGAAATCACTGGTAGTGGAGCATTTCTAAGAGAGGTTATTAGTTGTGGAGCATTTTTACGAGAAGAATTGTTAGTGCTTCTTCCCTTTAAATAGCCTACGTTCTATTCACAACAAAAATTTACTCTCCCCTCGAGTTATCGTGTTATTTTTTTTTCGCTTCTTTTTGTTTCGATTATTTGTTTCAATGTGCTTCGATCGGATGTCAAAACCATCATCTAGTATCAGACCTAGGATGCCGAAACAAATCCCGTCATCGCATAAAGTCATTGGGGGAGATGTGACGACGATACAGAACAGGAAGGAGAGGAGTGTAACGCTCACATACCCGTTACTCACAAAGAGTAACTATGTAGCGTGATCGATAAACATGCGTGTTAATTTGTTAGCACATGGTGTGTGGGTCGCCATCAAACATGGTGACAATATCGAGGAGCACAAGGACAAGATGACTCTTGTTGCCATCTACCAAGCAGTCGCGGAGGATGTCCTTCTCATGTTGGCAGAGAAAGACTCGACAAAGGCAGCATGGGAAACACCGCAAACAATGCATATGGGTGTATAATGGGTCAAGGAAGCAAAAGTGCAGATTTTGATGGGTGATTTCAAGGCTATCCGCATGAAAGACGGTTAGTCGGTGAACGACTTTACCATGAAGTTAACGATGATCGTCAACGGTATCTACTTGTTAAGAGACAAGGTGGAGGAGATCTCCTTCATCAAGAAGTTCCTCCAAGCTGTCTCCGAGATCATACAAATTGTTGCCTCCATTGAGTAGTCAACAACCTCAAGAACATGTCGGTTAAGGAGGTCGACAACCTTAAGAAAAAATGTTAACTAATTTGGAGTTCATCTGTGCTTTCAGATATAAATTTTCAATTGGGATGGATGATTACTTATGTAAAACTTCAACTACAGGCCATCTTATAAAATAGGCCTTGGGAATACTTGAATTCATCTCTGCTTTCGAATATAAATTTTCAATTGTTATGTACAATTACTAACTTGAGCCTTCAACTGCAGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGTATTGATCTTTGGATGAACTGCTACTCAAATTCTGTCGAGATAATTGATTGTGATGTATAATTGTGTTGAATTAAATGATGATCTTCACCCTCTTTTTATGCTTGTAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGTATTATCTCTCTCTCACACACACAATTTACTAAAATTTTAAACTGAAATAATTCCAACTTAAAAATATTTGCATTTTGATTTCTACTGTTAACTTCTGTTGCTTTTTGTGTGGAAAAAACTCCCAATGACTAAATATGTGTTCTACGAGACGAATTGAATTAATTTTAATCAGGAATATTAATGGTAGCTATCTGTAATTTTGTCGGACTTTTTCTTTTGTGGTCAGGAGGGAGTTAGGGTAGGAAGAATTTTAGTTTAGTTTGTTGAAGATCGTTAGGAGAGGAGTCCCAAGTCTGTTAATTCACGGGTTGATCATGGGTTTATACGAGGCATTATGGAGAAACCAAAAGCAAAATCATAAGAGCTTATGTTCAAAGTGAACAATATCATACTATTGTGAAGAGTCGTGATTTCTAACCTGGTGTCAGAGTCATACCCTTAACTTAGCCATGTCAATAGATTCCTTAAATGTCAAACAAAGAAGTTGAGAGTTTCTAATGTGTAGTCAAAAGTGACTCGAGTGCCAAAGTGAAGGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAATGGTTTTCTTTGGATGGGGGCTCTAGAGAAGGAGTCGAGTCTCAATTAAGGGGAGGTTGTTCGAAGGCTCCATAGATCTCAGGGGAAGCTCTATGGTATACTTTGTTCGAGGGGAGGATTGTTGAGATTTGTTGGGGGAGTCCCACGTTAGCTAATTAAGGAATGATCATGAGTTTATAAGTAATGAATACATATTGTCCTACTTTCATTTCAGGATTTTGGAGTTCTTGTAATTCCTACAGTCCCAGGTCCCCCTCCGAAACTAAGAACAGACATCTCGGAGCTACACGACTTTCGTGCAAAGGCTTTCAGTTTGCTCTCCATTGCTTCGGTCTCCGGAATCTGCCAAGTTCTCTCTCACTCTTTCTCTCTCTCTCTTGAAAGTTAAAGCAACCATATTGATGTATTTCTTGCCACCAACTAATGTGCAGGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAAGAAGTTGAGGGATTATCCACCGAATAATGTGAGAGATTTGATTTTTCATCCTTTCATGTATTCTTTGTATGATCATGTATTGTTTGTATGATTATGAATCAAGTTTTCTTTTTCTAGCAAGTTAGATTGTGGAATGAAATATTTGATCATGTATTCTTGGTATTTACCGCTAGAAGATATTGTCTTTTTTGGGCTTTCCCTTCCGGTCTTCCCCTC

mRNA sequence

AATATAATTTTTGGGCATTTCAGTAACCATTTTTTTAATTCAGCTCTCTTCCTCTTTCATTGTCCTGGTCTTCTCTGCAACTTCCAGCCATTATTAGGGTTTTCGGGGTGGATTTGGATCAGGGAGGCCGATCTCAGGTCTTGCAATGGCTGTGCAGGATCATGGAGCTTTCATAGAGAAATTCATCCTGCAACAGAGCTCCCCGTCCGATCCACTTCCCTTGACTGGCCTTACTTTCGCTGTTAAAGACATATTTGATATGGAGGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACAGCACCAGCCGTGTTGGCTATCCTAAGAGGAGGAGCCACTTGCATCGGCAAGACTATCATGGATGAAATGGCCTTCAGTATAAGTGGGGAAAACTTTCATTATGGCACACCCCGAAACCCATATGCATCAGATCGTATACCTGGAGGATCTTCCAGCGGCTCTGCTGTTGCTGTTGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCTTAGTTCCAAGACGAACCGATACTGGAGGTAGCGTAAGAGTGCCTGCTTCTTATTGTGGAATTCTTGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCCATGACGCAGAGCTTCGATACAGTAGGTTGGTTTGCCATCGATTCTGCCGTATTCAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAAGAAGTTGAGGGATTATCCACCGAATAATGTGAGAGATTTGATTTTTCATCCTTTCATGTATTCTTTGTATGATCATGTATTGTTTGTATGATTATGAATCAAGTTTTCTTTTTCTAGCAAGTTAGATTGTGGAATGAAATATTTGATCATGTATTCTTGGTATTTACCGCTAGAAGATATTGTCTTTTTTGGGCTTTCCCTTCCGGTCTTCCCCTC

Coding sequence (CDS)

ATGGCTGTGCAGGATCATGGAGCTTTCATAGAGAAATTCATCCTGCAACAGAGCTCCCCGTCCGATCCACTTCCCTTGACTGGCCTTACTTTCGCTGTTAAAGACATATTTGATATGGAGGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACAGCACCAGCCGTGTTGGCTATCCTAAGAGGAGGAGCCACTTGCATCGGCAAGACTATCATGGATGAAATGGCCTTCAGTATAAGTGGGGAAAACTTTCATTATGGCACACCCCGAAACCCATATGCATCAGATCGTATACCTGGAGGATCTTCCAGCGGCTCTGCTGTTGCTGTTGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCTTAGTTCCAAGACGAACCGATACTGGAGGTAGCGTAAGAGTGCCTGCTTCTTATTGTGGAATTCTTGGATTCCGGCCTTCACATGGTGTGGTCTCTACATCTGGAGTAATACCCATGACGCAGAGCTTCGATACAGTAGGTTGGTTTGCCATCGATTCTGCCGTATTCAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAA

Protein sequence

MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYSTLKQEVEASY

Homology

[Close]

BLAST of Carg27605 vs. NCBI nr
Match: KAG7023949.1 (Amidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.9 bits (2023), Expect = 6.6e-223
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSI 360
           SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSI
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSI 360

Query: 361 SLLAKHGSDGFLLNVVDTLYSTLKQEVEASY 392
           SLLAKHGSDGFLLNVVDTLYSTLKQEVEASY
Sbjct: 361 SLLAKHGSDGFLLNVVDTLYSTLKQEVEASY 391

[Close]

BLAST of Carg27605 vs. NCBI nr
Match: XP_022961361.1 (amidase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 740.7 bits (1911), Expect = 6.4e-210
Identity = 384/436 (88.07%), Postives = 384/436 (88.07%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE--------------- 360
           SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE               
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPP 360

Query: 361 ------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLNV 392
                                         ATIPLGLYNGLPVSISLLAKHGSDGFLLNV
Sbjct: 361 KLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNV 420

[Close]

BLAST of Carg27605 vs. NCBI nr
Match: XP_022961359.1 (amidase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 735.7 bits (1898), Expect = 2.0e-208
Identity = 384/438 (87.67%), Postives = 384/438 (87.67%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG-- 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAA 240

Query: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW 300
           DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW
Sbjct: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW 300

Query: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE------------- 360
           VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE             
Sbjct: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGP 360

Query: 361 --------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLL 392
                                           ATIPLGLYNGLPVSISLLAKHGSDGFLL
Sbjct: 361 PPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLL 420

[Close]

BLAST of Carg27605 vs. NCBI nr
Match: XP_022968724.1 (amidase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 731.9 bits (1888), Expect = 3.0e-207
Identity = 380/436 (87.16%), Postives = 380/436 (87.16%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQ SSPSDPLPL GLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE--------------- 360
           SYSSHLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLE               
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPP 360

Query: 361 ------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLNV 392
                                         ATIPLGLYNGLPVSISLLAKHGSDGFLLNV
Sbjct: 361 KLNTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNV 420

[Close]

BLAST of Carg27605 vs. NCBI nr
Match: XP_023516591.1 (amidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 728.0 bits (1878), Expect = 4.3e-206
Identity = 379/436 (86.93%), Postives = 380/436 (87.16%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDS VFKRVGRLLLQ PEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG +
Sbjct: 181 AIDSDVFKRVGRLLLQVPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGN 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFK NHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKSNHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE--------------- 360
           SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE               
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPP 360

Query: 361 ------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLNV 392
                                         ATIPLGLYNGLPVSISLLAKHGSDGFLLNV
Sbjct: 361 KLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNV 420

[Close]

BLAST of Carg27605 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 3.5e-118
Identity = 232/424 (54.72%), Postives = 281/424 (66.27%), Query Frame = 0


Query: 5   DHGAFIEKF-ILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAP 64
           D GAFIEK  I   S+ S P  L GLTFA+KDIFD+EG VTGFGNP+WLRTH  A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKL 124
            V ++L  GAT +G TIMDEMA+SI+GEN HYGTPRNP A DR+PGGSSSGSAVAV A+L
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 VDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAID 184
           VDFS+G       TDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFA D
Sbjct: 126 VDFSIG-------TDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARD 185

Query: 185 SAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH-LI 244
           +A  KRVG +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FG + ++
Sbjct: 186 TATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVV 245

Query: 245 KQISIGSYVEDKVPSLKHFMIEEN--DGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 304
           K++++G Y+   VPSLKHFM  ++     +  IPSL AL  S  LLQRHEFKINHG W+ 
Sbjct: 246 KKVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWIS 305

Query: 305 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL---------------- 364
           S     GPG SERI E IR  +DE  D  RSV+ EL  AL+ LL                
Sbjct: 306 SVKPEFGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPP 365

Query: 365 -----------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLNV 380
                                        + +IPLGL+  LPVS+SL+AK+GSDGFLL++
Sbjct: 366 HLQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSL 421

[Close]

BLAST of Carg27605 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-103
Identity = 204/432 (47.22%), Postives = 263/432 (60.88%), Query Frame = 0


Query: 5   DHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAPA 64
           D+GAF+E+F+L    PS  LPL GLTFA+KDIFD+ G VTGFGNP+W RTH PA  T+P 
Sbjct: 10  DYGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPV 69

Query: 65  VLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKLV 124
           VLA L  GAT +G TIMDEMA+SI+GEN HYGTP NP A  R+PGGSSSGSAVAV A LV
Sbjct: 70  VLAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLV 129

Query: 125 DFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDS 184
           DFSLG       TDTGGSVRVPA+YCGI G RPSHG+VS   VIPM Q FDTVGWF+ D 
Sbjct: 130 DFSLG-------TDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDL 189

Query: 185 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 244
           +   RV ++LL  P+    +PTQV I  DCF++     +R  Q    SV K F   ++  
Sbjct: 190 STLSRVTKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDN 249

Query: 245 ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 304
            ++G ++ D VPS+  F+ + ++     +P+L+ +      LQR +FK NH EWV +   
Sbjct: 250 RNLGDFISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKP 309

Query: 305 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL-------------------- 364
           +LGPG  ERI E I    +E+ +  ++++ E + ALAALL                    
Sbjct: 310 NLGPGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGM 369

Query: 365 -------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 392
                                    + +IPLG+ NGLPVS+SL+A+HG+D FLLNVV+ L
Sbjct: 370 EAAPLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEEL 429

[Close]

BLAST of Carg27605 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.2e-94
Identity = 188/434 (43.32%), Postives = 258/434 (59.45%), Query Frame = 0


Query: 4   QDHGAFIEKFIL----QQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPAN 63
           +D GAF+ K  L      + P  P PL+ L FA+ DIFD+EG+V+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAV 123
            TA AV A++  GATCIG T++DE+A+ ISGEN H+GTP NP   +R+PGGSSSG+AVAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 GAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGW 183
            A  VDFSLG        DT G VRVPA +CGILGFRPSHG VS  G+IP++ S DTVGW
Sbjct: 155 AANFVDFSLG-------VDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGW 214

Query: 184 FAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGD 243
           FA D  V +RVG +LLQ P V    P Q++IA+DCF+  ++P +R +Q  + + +KLFG 
Sbjct: 215 FAKDPDVLRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGK 274

Query: 244 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 303
            ++K I+   Y+  KV SLK   I++++G      SL  L      LQRHEF+  H EW+
Sbjct: 275 QVLKHINFEDYISSKVSSLKACSIQKSNGVLK-SSSLKLLANVMQSLQRHEFEHTHSEWM 334

Query: 304 RSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL--------------- 363
                 L P  S ++ E   V+  E  + S+SV+ ELRVA+ +LL               
Sbjct: 335 SIVKPDLHPAVSAQLHEKFEVSELE-IENSKSVRSELRVAVNSLLKDEGVLVIPTVADPP 394

Query: 364 ------------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLN 389
                                         + T+PLG ++  PVS+SL+A+HG D FLL+
Sbjct: 395 PKLGGKEFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLD 454

[Close]

BLAST of Carg27605 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.6e-94
Identity = 182/431 (42.23%), Postives = 253/431 (58.70%), Query Frame = 0


Query: 4   QDHGAFIEKFIL----QQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPAN 63
           +D GAFI+K +L    Q + P  P PLTGLTFAV D+FD+ GYVTGFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAV 123
            T+P V  ++ GGATC+GKT++DE AFSISGEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGW 183
               VDF+LG        DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGW
Sbjct: 152 ATNAVDFALG-------IDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGW 211

Query: 184 FAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGD 243
           FA D    +RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG 
Sbjct: 212 FARDPNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGR 271

Query: 244 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 303
            L+K  ++ +Y E KVPSLK F   +      +  S   L     LLQRHEF  NHG+W+
Sbjct: 272 QLLKHQNLETYFETKVPSLKEFARTKAIANTKVSTS-RLLANVMQLLQRHEFLQNHGDWI 331

Query: 304 RSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL--------------- 363
            +    + P    ++ E   + T+E T+   +++ E RVA+ +LL               
Sbjct: 332 NTVKPAIDPVILSQVCENPEL-TNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVP 391

Query: 364 ------------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLN 386
                                         + T+PLG +   P+S+S + +HG D FLL+
Sbjct: 392 PKLGSKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLD 451

[Close]

BLAST of Carg27605 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 6.6e-85
Identity = 174/434 (40.09%), Postives = 243/434 (55.99%), Query Frame = 0


Query: 4   QDHGAFIEKFILQQSSPSDP----LPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPAN 63
           +D GAF+++F L    P  P      L+GLTF++ D FD++ Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAV 123
           +TA  V  +L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGW 183
           GA+LVDFSLG        DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGW
Sbjct: 165 GAELVDFSLG-------IDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGW 224

Query: 184 FAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGD 243
           FA D +V  +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G 
Sbjct: 225 FASDPSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGY 284

Query: 244 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 303
              K +++G YV   VPSL  F  E++   ++   +L AL    + +QRHEFK NH EW 
Sbjct: 285 KTPKHVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWW 344

Query: 304 RSYSSHLGP-----------GKSERISEYIRVATDE------------------------ 363
           ++  S LGP            K+E I    RV  +                         
Sbjct: 345 QTCKSFLGPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPP 404

Query: 364 ----------NTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSISLLAKHGSDGFLLN 389
                     N  L R+  +    +++   + TIPLG +   P+S+SLL  +G D FLL+
Sbjct: 405 RLNTKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLD 464

[Close]

BLAST of Carg27605 vs. ExPASy TrEMBL
Match: A0A6J1HA58 (amidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.1e-210
Identity = 384/436 (88.07%), Postives = 384/436 (88.07%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE--------------- 360
           SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE               
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPP 360

Query: 361 ------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLNV 392
                                         ATIPLGLYNGLPVSISLLAKHGSDGFLLNV
Sbjct: 361 KLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNV 420

[Close]

BLAST of Carg27605 vs. ExPASy TrEMBL
Match: A0A6J1HDT1 (amidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 9.9e-209
Identity = 384/438 (87.67%), Postives = 384/438 (87.67%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG-- 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAA 240

Query: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW 300
           DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW
Sbjct: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW 300

Query: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE------------- 360
           VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE             
Sbjct: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGP 360

Query: 361 --------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLL 392
                                           ATIPLGLYNGLPVSISLLAKHGSDGFLL
Sbjct: 361 PPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLL 420

[Close]

BLAST of Carg27605 vs. ExPASy TrEMBL
Match: A0A6J1I0H5 (amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.4e-207
Identity = 380/436 (87.16%), Postives = 380/436 (87.16%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQ SSPSDPLPL GLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH 240

Query: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 300
           LIKQISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVR
Sbjct: 241 LIKQISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVR 300

Query: 301 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE--------------- 360
           SYSSHLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLE               
Sbjct: 301 SYSSHLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPP 360

Query: 361 ------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLNV 392
                                         ATIPLGLYNGLPVSISLLAKHGSDGFLLNV
Sbjct: 361 KLNTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNV 420

[Close]

BLAST of Carg27605 vs. ExPASy TrEMBL
Match: A0A6J1HYY1 (amidase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.6e-206
Identity = 380/438 (86.76%), Postives = 380/438 (86.76%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAVQDHGAFIEKFILQ SSPSDPLPL GLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG-- 240
           AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  
Sbjct: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAA 240

Query: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEW 300
           DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEW
Sbjct: 241 DHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEW 300

Query: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE------------- 360
           VRSYSSHLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLE             
Sbjct: 301 VRSYSSHLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGP 360

Query: 361 --------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLL 392
                                           ATIPLGLYNGLPVSISLLAKHGSDGFLL
Sbjct: 361 PPKLNTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLL 420

[Close]

BLAST of Carg27605 vs. ExPASy TrEMBL
Match: A0A0A0LZ24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.3e-183
Identity = 335/437 (76.66%), Postives = 357/437 (81.69%), Query Frame = 0


Query: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60
           MAV D+GAFIEKF+LQ SSPSD LPL+GLTFAVKDIFD+EGYV GFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120
           TAPAVL ILRGGATCIGKTIMDEMAFSI+GENFHYGTP+NP+ASDRIPGGSSSGS VAV 
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSLG       TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLG-------TDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 AIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGD 240
           A DSA+ KRVG LLLQEPE+EHYKPT+V IAEDCFK LS+I SERLTQAFV+SV+KLFG 
Sbjct: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 300
           HLIKQIS+G YVEDKVPSLKHFMIEENDGYKH IPSLAALVRSK  LQRHEF INHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300

Query: 301 RSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLE-------------- 360
           RSY SHLGPG SERI E+IR  TDEN DLSRS+QIELR ALAALLE              
Sbjct: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPP 360

Query: 361 -------------------------------ATIPLGLYNGLPVSISLLAKHGSDGFLLN 392
                                           ++PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420

[Close]

BLAST of Carg27605 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 426.4 bits (1095), Expect = 2.5e-119
Identity = 232/424 (54.72%), Postives = 281/424 (66.27%), Query Frame = 0


Query: 5   DHGAFIEKF-ILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAP 64
           D GAFIEK  I   S+ S P  L GLTFA+KDIFD+EG VTGFGNP+WLRTH  A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKL 124
            V ++L  GAT +G TIMDEMA+SI+GEN HYGTPRNP A DR+PGGSSSGSAVAV A+L
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 VDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAID 184
           VDFS+G       TDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFA D
Sbjct: 126 VDFSIG-------TDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARD 185

Query: 185 SAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH-LI 244
           +A  KRVG +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FG + ++
Sbjct: 186 TATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVV 245

Query: 245 KQISIGSYVEDKVPSLKHFMIEEN--DGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVR 304
           K++++G Y+   VPSLKHFM  ++     +  IPSL AL  S  LLQRHEFKINHG W+ 
Sbjct: 246 KKVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWIS 305

Query: 305 SYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL---------------- 364
           S     GPG SERI E IR  +DE  D  RSV+ EL  AL+ LL                
Sbjct: 306 SVKPEFGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPP 365

Query: 365 -----------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLNV 380
                                        + +IPLGL+  LPVS+SL+AK+GSDGFLL++
Sbjct: 366 HLQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSL 421

[Close]

BLAST of Carg27605 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 346.3 bits (887), Expect = 3.3e-95
Identity = 182/431 (42.23%), Postives = 253/431 (58.70%), Query Frame = 0


Query: 4   QDHGAFIEKFIL----QQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPAN 63
           +D GAFI+K +L    Q + P  P PLTGLTFAV D+FD+ GYVTGFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAV 123
            T+P V  ++ GGATC+GKT++DE AFSISGEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGW 183
               VDF+LG        DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGW
Sbjct: 152 ATNAVDFALG-------IDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGW 211

Query: 184 FAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGD 243
           FA D    +RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG 
Sbjct: 212 FARDPNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGR 271

Query: 244 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 303
            L+K  ++ +Y E KVPSLK F   +      +  S   L     LLQRHEF  NHG+W+
Sbjct: 272 QLLKHQNLETYFETKVPSLKEFARTKAIANTKVSTS-RLLANVMQLLQRHEFLQNHGDWI 331

Query: 304 RSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALL--------------- 363
            +    + P    ++ E   + T+E T+   +++ E RVA+ +LL               
Sbjct: 332 NTVKPAIDPVILSQVCENPEL-TNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVP 391

Query: 364 ------------------------------EATIPLGLYNGLPVSISLLAKHGSDGFLLN 386
                                         + T+PLG +   P+S+S + +HG D FLL+
Sbjct: 392 PKLGSKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLD 451

[Close]

BLAST of Carg27605 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 315.8 bits (808), Expect = 4.7e-86
Identity = 174/434 (40.09%), Postives = 243/434 (55.99%), Query Frame = 0


Query: 4   QDHGAFIEKFILQQSSPSDP----LPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPAN 63
           +D GAF+++F L    P  P      L+GLTF++ D FD++ Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAV 123
           +TA  V  +L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GAKLVDFSLGKFLVPRRTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGW 183
           GA+LVDFSLG        DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGW
Sbjct: 165 GAELVDFSLG-------IDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGW 224

Query: 184 FAIDSAVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGD 243
           FA D +V  +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G 
Sbjct: 225 FASDPSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGY 284

Query: 244 HLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWV 303
              K +++G YV   VPSL  F  E++   ++   +L AL    + +QRHEFK NH EW 
Sbjct: 285 KTPKHVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWW 344

Query: 304 RSYSSHLGP-----------GKSERISEYIRVATDE------------------------ 363
           ++  S LGP            K+E I    RV  +                         
Sbjct: 345 QTCKSFLGPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPP 404

Query: 364 ----------NTDLSRSVQIELRVALAALLEATIPLGLYNGLPVSISLLAKHGSDGFLLN 389
                     N  L R+  +    +++   + TIPLG +   P+S+SLL  +G D FLL+
Sbjct: 405 RLNTKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLD 464

[Close]

BLAST of Carg27605 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 101.3 bits (251), Expect = 1.8e-21
Identity = 60/156 (38.46%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 25  PLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAPAVLAILRGGATCIGKTIMDEM 84
           PL G+   VKD    +G  +   +       PP + T  AV  I   G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDAT--AVKKIKELGGIVVGKTNMDEF 166

Query: 85  AFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKLVDFSLGKFLVPRRTDTGGSVR 144
               + E   +    NP+   R+PGGSS GSA AV A+    SLG       +DTGGSVR
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLG-------SDTGGSVR 226

Query: 145 VPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 181
            PAS+CG++G +P++G VS  G++    S D +G F
Sbjct: 227 QPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

[Close]

BLAST of Carg27605 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 91.7 bits (226), Expect = 1.4e-18
Identity = 53/153 (34.64%), Postives = 76/153 (49.67%), Query Frame = 0


Query: 26  LTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQTAPAVLAILRGGATCIGKTIMDEMA 85
           L G+   +KD  D   + T  G   WL       + +  V  +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  FSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVGAKLVDFSLGKFLVPRRTDTGGSVRV 145
              +G N +YGT RNP+   R  GGSSSGSA  V A L   +LG       TD GGSVR+
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALG-------TDGGGSVRI 316

Query: 146 PASYCGILGFRPSHGVVSTSGVIPMTQSFDTVG 179
           P++ CGI G + ++G    +G +    + + +G
Sbjct: 317 PSALCGITGLKTTYGRTDMTGSLCEGGTVEIIG 341

The following BLAST results are available for this feature:

BLAST of Carg27605 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023949.1	6.6e-223	100.00	Amidase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961361.1	6.4e-210	88.07	amidase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022961359.1	2.0e-208	87.67	amidase 1-like isoform X1 [Cucurbita moschata]	[more]
XP_022968724.1	3.0e-207	87.16	amidase 1 isoform X2 [Cucurbita maxima]	[more]
XP_023516591.1	4.3e-206	86.93	amidase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg27605 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FR37	3.5e-118	54.72	Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1	[more]
Q7XTK3	1.4e-103	47.22	Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1	[more]
Q9MUK5	1.2e-94	43.32	Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=...	[more]
Q9LVH5	4.6e-94	42.23	Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6...	[more]
F4KCL7	6.6e-85	40.09	Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64...	[more]

BLAST of Carg27605 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HA58	3.1e-210	88.07	amidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=...	[more]
A0A6J1HDT1	9.9e-209	87.67	amidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=...	[more]
A0A6J1I0H5	1.4e-207	87.16	amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1	[more]
A0A6J1HYY1	4.6e-206	86.76	amidase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1	[more]
A0A0A0LZ24	9.3e-183	76.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=3 SV=1	[more]

BLAST of Carg27605 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08980.1	2.5e-119	54.72	amidase 1	[more]
AT3G17970.1	3.3e-95	42.23	translocon at the outer membrane of chloroplasts 64-III	[more]
AT5G09420.1	4.7e-86	40.09	translocon at the outer membrane of chloroplasts 64-V	[more]
AT3G25660.1	1.8e-21	38.46	Amidase family protein	[more]
AT5G64440.1	1.4e-18	34.64	fatty acid amide hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 22..182 e-value: 1.7E-46 score: 159.2
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 12..385 e-value: 2.3E-65 score: 223.4
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 20..379
None	No IPR available	PANTHER	PTHR46310	AMIDASE 1	coord: 4..345 coord: 347..388
None	No IPR available	PANTHER	PTHR46310:SF7	AMIDASE 1	coord: 4..345 coord: 347..388

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27605-RA	Carg27605-RA	mRNA