Carg27544 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27544
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mitogen-activated protein kinase kinase kinase 18-like
Location	Carg_Chr11: 12119711 .. 12120802 (+)
RNA-Seq Expression	Carg27544
Synteny	Carg27544

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCTGGATCCGACTCGCCGCCGTCGGCCACGGCTCCTCCGCCACCGTCTACCTTGCCTCCGCCGCCCCTGCCGGAGACGTTTTTGCTGTCAAGTCCGCCGAGCTTCCCGAATCGGAGTTCTTGAAAACAGAGCAGAGGATTCTTTCTTCTTTGTCCCACCCTTCCATTGTGAGCTACAGGGGATTCGATGTAACGAGAGAGAATGGGAAGCTGATGTATAATCTCTTCATGGAGTACGCCACCGGAGGTACACTCGCCGACGAAATTCTCCGGTGCGGCGGCTGGATTAAAGAGACGGCGGCGGGATTCTACACGCGTGAAATTGTGAAAGGATTGGAGTATTTGCACAAAAGGGGTTTGGTGCATTGTGATATTAAAGCTAAGAATATTTTAATCGGGGAAGATGGTTTGAAGATCGCGGATTTCGGATGCTCGAAATGGGTTAATGAACCGGCGGCTATCGGTGGAACGCCGATGTTCATGGCGCCGGAAGTAGCACGTGGGGAAAAACAGGGGATTTCCTCTGACATTTGGTCACTTGGGTGTACTTTAATCGAAATGGTCAACGGTGCTCCACCGTGGCCAACCGACGGCGACCCAGTTTCAGTACTGTACCGGATAGGATATTCCGGCGAGTCGCCAGAAATTCCAAGCTTTCTATCGGAGAAAGCGAAGGATTTTCTTGGAAAATGCTTGAGAAGAAACCCAACAGAACGCTGGAGCGCGAGCCAGCTTCTTGAACATCCATTCTTAGAGGACACGAATTCTGAGTGGTTCGAGGAATCCAAGGAATTAGATTCAAATTCTCCGACAAGTATCTTGGATCAAGGCGTTTGGAATTCGCAAAGTGAGCTGAAAACAGAGGAATGGGACGACGACAGAATCAGACGGCTGTCGATGTTCCGATGGGAAATCAAGTGGGAATCGGACGGTGAGAATTGGATGACGATCAGAGGAAGGTGCGTGGACGGTGAAGATGAGGCGGAATCCATTAGTGGGCAAACCAAAAGCTTGGAATTGTTTGATAGAACAGTTAGGTTTAGAGTAAGTGATGATGTAATTAGTGATCTTGTTTCATTAATTAGTTGA

mRNA sequence

ATGACCTGGATCCGACTCGCCGCCGTCGGCCACGGCTCCTCCGCCACCGTCTACCTTGCCTCCGCCGCCCCTGCCGGAGACGTTTTTGCTGTCAAGTCCGCCGAGCTTCCCGAATCGGAGTTCTTGAAAACAGAGCAGAGGATTCTTTCTTCTTTGTCCCACCCTTCCATTGTGAGCTACAGGGGATTCGATGTAACGAGAGAGAATGGGAAGCTGATGTATAATCTCTTCATGGAGTACGCCACCGGAGGTACACTCGCCGACGAAATTCTCCGGTGCGGCGGCTGGATTAAAGAGACGGCGGCGGGATTCTACACGCGTGAAATTGTGAAAGGATTGGAGTATTTGCACAAAAGGGGTTTGGTGCATTGTGATATTAAAGCTAAGAATATTTTAATCGGGGAAGATGGTTTGAAGATCGCGGATTTCGGATGCTCGAAATGGGTTAATGAACCGGCGGCTATCGGTGGAACGCCGATGTTCATGGCGCCGGAAGTAGCACGTGGGGAAAAACAGGGGATTTCCTCTGACATTTGGTCACTTGGGTGTACTTTAATCGAAATGGTCAACGGTGCTCCACCGTGGCCAACCGACGGCGACCCAGTTTCAGTACTGTACCGGATAGGATATTCCGGCGAGTCGCCAGAAATTCCAAGCTTTCTATCGGAGAAAGCGAAGGATTTTCTTGGAAAATGCTTGAGAAGAAACCCAACAGAACGCTGGAGCGCGAGCCAGCTTCTTGAACATCCATTCTTAGAGGACACGAATTCTGAGTGGTTCGAGGAATCCAAGGAATTAGATTCAAATTCTCCGACAAGTATCTTGGATCAAGGCGTTTGGAATTCGCAAAGTGAGCTGAAAACAGAGGAATGGGACGACGACAGAATCAGACGGCTGTCGATGTTCCGATGGGAAATCAAGTGGGAATCGGACGGTGAGAATTGGATGACGATCAGAGGAAGGTGCGTGGACGGTGAAGATGAGGCGGAATCCATTAGTGGGCAAACCAAAAGCTTGGAATTGTTTGATAGAACAGTTAGGTTTAGAGTAAGTGATGATGTAATTAGTGATCTTGTTTCATTAATTAGTTGA

Coding sequence (CDS)

ATGACCTGGATCCGACTCGCCGCCGTCGGCCACGGCTCCTCCGCCACCGTCTACCTTGCCTCCGCCGCCCCTGCCGGAGACGTTTTTGCTGTCAAGTCCGCCGAGCTTCCCGAATCGGAGTTCTTGAAAACAGAGCAGAGGATTCTTTCTTCTTTGTCCCACCCTTCCATTGTGAGCTACAGGGGATTCGATGTAACGAGAGAGAATGGGAAGCTGATGTATAATCTCTTCATGGAGTACGCCACCGGAGGTACACTCGCCGACGAAATTCTCCGGTGCGGCGGCTGGATTAAAGAGACGGCGGCGGGATTCTACACGCGTGAAATTGTGAAAGGATTGGAGTATTTGCACAAAAGGGGTTTGGTGCATTGTGATATTAAAGCTAAGAATATTTTAATCGGGGAAGATGGTTTGAAGATCGCGGATTTCGGATGCTCGAAATGGGTTAATGAACCGGCGGCTATCGGTGGAACGCCGATGTTCATGGCGCCGGAAGTAGCACGTGGGGAAAAACAGGGGATTTCCTCTGACATTTGGTCACTTGGGTGTACTTTAATCGAAATGGTCAACGGTGCTCCACCGTGGCCAACCGACGGCGACCCAGTTTCAGTACTGTACCGGATAGGATATTCCGGCGAGTCGCCAGAAATTCCAAGCTTTCTATCGGAGAAAGCGAAGGATTTTCTTGGAAAATGCTTGAGAAGAAACCCAACAGAACGCTGGAGCGCGAGCCAGCTTCTTGAACATCCATTCTTAGAGGACACGAATTCTGAGTGGTTCGAGGAATCCAAGGAATTAGATTCAAATTCTCCGACAAGTATCTTGGATCAAGGCGTTTGGAATTCGCAAAGTGAGCTGAAAACAGAGGAATGGGACGACGACAGAATCAGACGGCTGTCGATGTTCCGATGGGAAATCAAGTGGGAATCGGACGGTGAGAATTGGATGACGATCAGAGGAAGGTGCGTGGACGGTGAAGATGAGGCGGAATCCATTAGTGGGCAAACCAAAAGCTTGGAATTGTTTGATAGAACAGTTAGGTTTAGAGTAAGTGATGATGTAATTAGTGATCTTGTTTCATTAATTAGTTGA

Protein sequence

MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRGLVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWSLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLSMFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVSLIS

Homology

[Close]

BLAST of Carg27544 vs. NCBI nr
Match: KAG7022856.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. NCBI nr
Match: KAG6589157.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.5 bits (1913), Expect = 3.5e-210
Identity = 362/363 (99.72%), Postives = 363/363 (100.00%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENG+LMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGRLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. NCBI nr
Match: XP_023531740.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.2 bits (1907), Expect = 1.7e-209
Identity = 361/363 (99.45%), Postives = 362/363 (99.72%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTW RLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWTRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISG+TKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGRTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. NCBI nr
Match: XP_022930728.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 736.1 bits (1899), Expect = 1.5e-208
Identity = 360/363 (99.17%), Postives = 360/363 (99.17%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTW RLAAVG GSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWTRLAAVGQGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WS SQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSTSQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. NCBI nr
Match: XP_022988825.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 734.2 bits (1894), Expect = 5.5e-208
Identity = 358/363 (98.62%), Postives = 360/363 (99.17%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTW RLAAVGHGSSATVYLASAAP+GDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWTRLAAVGHGSSATVYLASAAPSGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRC GWIKETAAGFYTR+IVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCDGWIKETAAGFYTRQIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKI DFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKITDFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.9e-86
Identity = 176/366 (48.09%), Postives = 229/366 (62.57%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R   +G GS+ATVY A+   + ++ AVKS+E+  SEFL+ E +ILSSLS P ++ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFDVTRE-NGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKR 120
           RG +  RE NG +MYNL MEYA  GTL D   + GG + ET    YTR+I+KGLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDIKAKNILIGEDG-LKIADFGCSKWVNE--PAAIGGTPMFMAPEVARGEKQGISS 180
           G+VHCD+K  N++I E G  KIADFGC+K V+    + + GTP FMAPEVARGEKQG  S
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DIWSLGCTLIEMVNGAPPW---PTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCL 240
           DIW++GCT+IEMV G+PPW    +  DPVSVLYR+GYS E+PE+P  L+E+AKDFL KCL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 RRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDD 300
           +R   ERW+A+QLL HPFL             L SNSPTS+ DQ  W S  E + EE ++
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 --------DRIRRLSMFRWEI------------KWESDGENWMTIRGRCVDGEDEAESIS 340
                   DR+     +   I            + + +G +W+ +R RC     E   IS
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRARC-----EGTMIS 360

[Close]

BLAST of Carg27544 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.3e-86
Identity = 173/341 (50.73%), Postives = 223/341 (65.40%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R   +G GS+ATV  A+   +G+  AVKSAE   SEFL+ E +ILSSL+ P ++ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFDVTRE----NGK-LMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEY 120
           RG ++TRE    NG+   Y+L MEYA  GTL D   + GG+I E     YTR+I+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KRGLVHCDIKAKNILIGEDG-LKIADFGCSKWVNEP---AAIGGTPMFMAPEVARGE 180
           +H  +G+ HCDIK  N+L+GE+G  KIADFGC+KWV EP     + GTP FMAPE ARGE
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWV-EPEITEPVRGTPAFMAPEAARGE 180

Query: 181 KQGISSDIWSLGCTLIEMVNGAPPW--PTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDF 240
           +QG  SDIW++GCT+IEMV G+ PW      DPVSVLYR+GY GE PE+P  L+E+AKDF
Sbjct: 181 RQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDF 240

Query: 241 LGKCLRRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKT 300
           LGKCL++  TERW+ASQLL HPFL +   E       L +NSPTS+ DQ  W S  E  +
Sbjct: 241 LGKCLKKEATERWTASQLLNHPFLVNKEPELV---TGLVTNSPTSVTDQMFWRSVEEEVS 300

Query: 301 EE----WDDDRIRRLSMFRW------EIKWESDGENWMTIR 320
           E+    W+     R+ +  W      E  W+ DGE+W+T+R
Sbjct: 301 EDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

[Close]

BLAST of Carg27544 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.2e-49
Identity = 127/344 (36.92%), Postives = 190/344 (55.23%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLAS-AAPAGD---VFAVKSAELPESEFLKTEQRILSSLSH-P 60
           M W+R   +G G+ +TV  A+ +  +GD   + AVKS +   +  L  E+ +L SL   P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  SIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGG-WIKETAAGFYTREIVKGLE 120
            I+   G D T ENG+ M+NL +EYA+ G+LA  + + GG  + E+    +T  +++GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YLHKRGLVHCDIKAKNILIGEDG-LKIADFGCSKWVN-------EPAAIGGTPMFMAPEV 180
           ++H +G  HCDIK  NIL+  DG +KIADFG +  V+       +   I GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGISSDIWSLGCTLIEMVNGAPPWPT-DGDP-VSVLYRIGYSGESPEIPSFLSEK 240
               + G ++D+W+LGC ++EM +G   W   +G   +S+L RIG   E P+IP  LSE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 AKDFLGKCLRRNPTERWSASQLLEHPF----LEDTNSEWF-----EESKELDSNSPTSIL 300
            KDFL KC  ++P +RW+A  LL H F    LED + E F     +E K L   SP    
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVL--MSPKCPF 300

Query: 301 DQGVWNSQSELKTEEWDDDRIRRLSMFRWEIKWESDGENWMTIR 320
           +   W+S +      +D    R  S+    I   S G +W+T+R
Sbjct: 301 EFDDWDSFTLDSNPSFDSPVERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of Carg27544 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.1e-47
Identity = 107/274 (39.05%), Postives = 156/274 (56.93%), Query Frame = 0


Query: 3   WIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPES-----------EFLKTEQRILSS 62
           W +   +G G+   VY+     +G++ A+K   +  +             L+ E  +L +
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKN 139

Query: 63  LSHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVK 122
           LSHP+IV Y G    RE G L  N+ +E+  GG+++  + + G +  E+    YT++++ 
Sbjct: 140 LSHPNIVRYLG--TAREAGSL--NILLEFVPGGSISSLLGKFGSF-PESVIRMYTKQLLL 199

Query: 123 GLEYLHKRGLVHCDIKAKNILIGEDG-LKIADFGCSKWVNEPA------AIGGTPMFMAP 182
           GLEYLHK G++H DIK  NIL+   G +K+ADFG SK V E A      ++ GTP +MAP
Sbjct: 200 GLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAP 259

Query: 183 EVARGEKQGISSDIWSLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEK 242
           EV        S+DIWS+GCT+IEM  G PPW      V+ L+ IG +   P IP  LS +
Sbjct: 260 EVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSAE 319

Query: 243 AKDFLGKCLRRNPTERWSASQLLEHPFLEDTNSE 259
           +KDFL KCL++ P  R SAS LL+HPF+   + E
Sbjct: 320 SKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQE 348

[Close]

BLAST of Carg27544 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 4.3e-46
Identity = 106/301 (35.22%), Postives = 165/301 (54.82%), Query Frame = 0


Query: 3   WIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESE----------FLKTEQRILSSL 62
           W +   +G G   +VYL      G++FAVK  E+ +                E  ++ SL
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSL 229

Query: 63  SHPSIVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKG 122
            H +IV Y G  + +       ++F+EY  GG+++  + + G +  E     YT++I++G
Sbjct: 230 RHDNIVRYLGTSLDQS----FLSVFLEYIPGGSISSLLGKFGAF-SENVIKVYTKQILQG 289

Query: 123 LEYLHKRGLVHCDIKAKNILIGEDGL-KIADFGCSK----WVNEPAAIGGTPMFMAPEVA 182
           L +LH   ++H DIK  NILI   G+ K++DFGCSK     V++  ++ GTP +MAPEV 
Sbjct: 290 LSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVI 349

Query: 183 RGEKQGISSDIWSLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKD 242
           +    G SSDIWSLGC ++EM    PPW    +  +V+Y I  S   P IPS +S++A D
Sbjct: 350 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 409

Query: 243 FLGKCLRRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELK 289
           FL  C +R+P ER  A+QLL+HPF+ +      +++ +L + SPT+ L     N+ +   
Sbjct: 410 FLNLCFKRDPKERPDANQLLKHPFIMN-----LDDNIQLPTISPTTTLSTNTTNTTATTT 460

[Close]

BLAST of Carg27544 vs. ExPASy TrEMBL
Match: A0A6J1EW68 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111437119 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.0e-209
Identity = 360/363 (99.17%), Postives = 360/363 (99.17%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTW RLAAVG GSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWTRLAAVGQGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WS SQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSTSQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. ExPASy TrEMBL
Match: A0A6J1JKQ2 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111486056 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 2.7e-208
Identity = 358/363 (98.62%), Postives = 360/363 (99.17%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           MTW RLAAVGHGSSATVYLASAAP+GDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY
Sbjct: 1   MTWTRLAAVGHGSSATVYLASAAPSGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYATGGTLADEILRC GWIKETAAGFYTR+IVKGLEYLHKRG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCDGWIKETAAGFYTRQIVKGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180
           LVHCDIKAKNILIGEDGLKI DFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS
Sbjct: 121 LVHCDIKAKNILIGEDGLKITDFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIWS 180

Query: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240
           LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER
Sbjct: 181 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 240

Query: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300
           WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS
Sbjct: 241 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDDDRIRRLS 300

Query: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360
           MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS
Sbjct: 301 MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVS 360

Query: 361 LIS 364
           LIS
Sbjct: 361 LIS 363

[Close]

BLAST of Carg27544 vs. ExPASy TrEMBL
Match: A0A6J1GLZ4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111455196 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 2.0e-139
Identity = 255/335 (76.12%), Postives = 286/335 (85.37%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W RLAAVGHGS ATVYLA+++ +GD+FAVKSAEL  S+FL+TEQRILSSLSHPSIV Y
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           +GFDVT ENGKLMYNLFMEYA GG+LADEI+R GG IKE  A FYTR+IV+GLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNE-PAAIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCD+KA+NILIG DGLKIADFGCSKWV E PAA+ GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 SLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTE 240
           +LGCTLIEMV+GAPPWPT GDPVS LYRI YSG+SPEIPSFLSEKAKDFLGKCLRRNPTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSASQLLEHPFLEDTNSEWFEESKEL--DSNSPTSILDQGVWN-----SQSELKTEEWD 300
           RWSA++LL+HPFL +  S  FE  +EL  D  SPTSILDQG WN     SQ+ LKTE W+
Sbjct: 241 RWSANRLLKHPFLGEL-SFGFEIIRELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWE 300

Query: 301 DDRIRRLSMFRWEIKWESDGENWMTIRGRCVDGED 328
           DDRI+RLSMF  EIKWE   +NW+TIR  CV+ ++
Sbjct: 301 DDRIKRLSMFSGEIKWEMRDDNWITIRS-CVNDDN 333

[Close]

BLAST of Carg27544 vs. ExPASy TrEMBL
Match: A0A5A7T9T3 (Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004350 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.7e-138
Identity = 270/379 (71.24%), Postives = 292/379 (77.04%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R + VG+GSSATVYLAS A +G++FAVKSAEL +S+FLKTEQRILSSLSHPSIV Y
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           RGFDVTRENGKLMYNLFMEYA GGTL DEI R GG IKE  A FYTREIV+GLEYLHK+G
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNEPAAI-GGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILI  DGLKIADFGCSKWV E  A+ GGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 SLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTE 240
           +LGCTLIEMV GA PW T  DPVSVLYRIGYSGESPE+PSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLEDTNSEWFEESKELD--SNSPTSILDQGVWNSQSE-----LKTEEW- 300
           RWSASQLLEHPFL + +S   EE KEL   S SPTSILDQ +WNS  E     LKTE+W 
Sbjct: 241 RWSASQLLEHPFLGELSS-GLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWD 300

Query: 301 ----DDDRIRRLSMFRWEIKWESDGENWMTIRGRCVDGEDEAE----------------S 351
               DDDRI RL+ F  EIKWE   ENW+TIR   VDGEDE E                 
Sbjct: 301 DEDEDDDRIERLATFSGEIKWELGDENWITIRS-YVDGEDEDELNFSRNDLDDLEEEGRI 360

[Close]

BLAST of Carg27544 vs. ExPASy TrEMBL
Match: A0A0A0K6U1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043040 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.7e-138
Identity = 266/373 (71.31%), Postives = 291/373 (78.02%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R + VG+GSSATVYLAS A +G +FAVKSAEL +S+FLK E+R+LSSLS+PSIV Y
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIERRVLSSLSNPSIVGY 60

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
           +GFDVTRENGKLMYNLFMEYA GGTLADEI R GG IKE  A FYTREIV+GLEYLHK+G
Sbjct: 61  KGFDVTRENGKLMYNLFMEYAAGGTLADEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIGEDGLKIADFGCSKWVNE-PAAIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILI  DGLKIADFGCS+WV E  A IGGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCESEAVIGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 SLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTE 240
           +LGCTLIEMV GAPPW    DPVSVLYRIGYSGESPEIPSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLEDTNSEWFEESKELD--SNSPTSILDQGVWNSQSE-----LKTEEWD 300
           RWSASQLLEHPFL + +S   EE KEL   S SPTSILDQ +WNS  E     L+TE+WD
Sbjct: 241 RWSASQLLEHPFLGELSS-GLEEIKELHLYSESPTSILDQSLWNSLEEESETLLRTEQWD 300

Query: 301 DDRIRRLSMFRWEIKWESDGENWMTIRGRCVDGEDEAE---------------SISGQTK 351
           DDRI RL+ F  EIKWE   ENW+TIR   VDGEDE E                +S + +
Sbjct: 301 DDRIERLATFSGEIKWELGDENWITIRS-YVDGEDEDELNFSRNDLDLVEEGRIVSEEIQ 360

[Close]

BLAST of Carg27544 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 176/366 (48.09%), Postives = 229/366 (62.57%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R   +G GS+ATVY A+   + ++ AVKS+E+  SEFL+ E +ILSSLS P ++ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFDVTRE-NGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKR 120
           RG +  RE NG +MYNL MEYA  GTL D   + GG + ET    YTR+I+KGLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDIKAKNILIGEDG-LKIADFGCSKWVNE--PAAIGGTPMFMAPEVARGEKQGISS 180
           G+VHCD+K  N++I E G  KIADFGC+K V+    + + GTP FMAPEVARGEKQG  S
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DIWSLGCTLIEMVNGAPPW---PTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCL 240
           DIW++GCT+IEMV G+PPW    +  DPVSVLYR+GYS E+PE+P  L+E+AKDFL KCL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 RRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKTEEWDD 300
           +R   ERW+A+QLL HPFL             L SNSPTS+ DQ  W S  E + EE ++
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 --------DRIRRLSMFRWEI------------KWESDGENWMTIRGRCVDGEDEAESIS 340
                   DR+     +   I            + + +G +W+ +R RC     E   IS
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRARC-----EGTMIS 360

[Close]

BLAST of Carg27544 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 320.1 bits (819), Expect = 2.3e-87
Identity = 173/341 (50.73%), Postives = 223/341 (65.40%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           M W R   +G GS+ATV  A+   +G+  AVKSAE   SEFL+ E +ILSSL+ P ++ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFDVTRE----NGK-LMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEY 120
           RG ++TRE    NG+   Y+L MEYA  GTL D   + GG+I E     YTR+I+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KRGLVHCDIKAKNILIGEDG-LKIADFGCSKWVNEP---AAIGGTPMFMAPEVARGE 180
           +H  +G+ HCDIK  N+L+GE+G  KIADFGC+KWV EP     + GTP FMAPE ARGE
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWV-EPEITEPVRGTPAFMAPEAARGE 180

Query: 181 KQGISSDIWSLGCTLIEMVNGAPPW--PTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDF 240
           +QG  SDIW++GCT+IEMV G+ PW      DPVSVLYR+GY GE PE+P  L+E+AKDF
Sbjct: 181 RQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDF 240

Query: 241 LGKCLRRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWNSQSELKT 300
           LGKCL++  TERW+ASQLL HPFL +   E       L +NSPTS+ DQ  W S  E  +
Sbjct: 241 LGKCLKKEATERWTASQLLNHPFLVNKEPELV---TGLVTNSPTSVTDQMFWRSVEEEVS 300

Query: 301 EE----WDDDRIRRLSMFRW------EIKWESDGENWMTIR 320
           E+    W+     R+ +  W      E  W+ DGE+W+T+R
Sbjct: 301 EDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

[Close]

BLAST of Carg27544 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-86
Identity = 169/363 (46.56%), Postives = 233/363 (64.19%), Query Frame = 0


Query: 3   WIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSYRG 62
           WIR   +G GS+ATV L     +GD FAVKSAE   S FL+ EQ ILS LS P IV Y G
Sbjct: 6   WIRGPIIGRGSTATVSL-GITNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRGLV 122
            +VT+EN KLMYNL MEY +GG+L D I   GG + E     YTR+I+KGL YLH +G+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKAKNILIGEDGLKIADFGCSKWV--NEPAAIGGTPMFMAPEVARGEKQGISSDIWS 182
           HCD+K++N++IG +  KI D GC+K V  NE     GTP FM+PEVARGE+Q   +D+W+
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 LGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTER 242
           LGCT+IEM  G+ PWP   D V+ +Y+IG++GESP IP +LSEK +DFL KCLR++P +R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVWN----SQSELKTEE------ 302
           W+  +LL+HPFL++ +++  +    L+S+SP+++LDQ  W+    S+S    E+      
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDLCETSRSRFIKEDHEDPFA 305

Query: 303 ------WDD-----DRIRRLS--MFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTK 341
                 WDD     DRI++L+      E  WE++G  W+ +RG      +E +    +  
Sbjct: 306 NSTNFLWDDDSLPGDRIKKLAGDESSGEPDWETNG--WIEVRGEIEKRNEEEDENCVEAT 365

[Close]

BLAST of Carg27544 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-80
Identity = 162/399 (40.60%), Postives = 245/399 (61.40%), Query Frame = 0


Query: 1   MTWIRLAAVGHGSSATVYLASAAPAGDVFAVKSAELPESEFLKTEQRILSSLSHPSIVSY 60
           + W R   +G GS+ATV +A ++ +G++FAVKSA+L  S  L+ EQ ILS+LS P +V Y
Sbjct: 3   INWTRGPIIGRGSTATVSIAISS-SGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  RGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIKETAAGFYTREIVKGLEYLHKRG 120
            G  +TRE+  L+YN+ MEY +GG L D I   GG + E     YTR+I+ GL YLH+RG
Sbjct: 63  IGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERG 122

Query: 121 LVHCDIKAKNILIGEDG-LKIADFGCSKWVNEPAAIGGTPMFMAPEVARGEKQGISSDIW 180
           +VHCD+K+ N+L+ E+G LKIAD GC+K V++ +   GTP FMAPEVARGE+Q   +D+W
Sbjct: 123 IVHCDLKSHNVLVEENGVLKIADMGCAKSVDK-SEFSGTPAFMAPEVARGEEQRFPADVW 182

Query: 181 SLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGKCLRRNPTE 240
           +LGCT+IEM+ G+ PWP   D V+ +Y+IG+SGESP IP+++S+KAKDFL  CL+ +  +
Sbjct: 183 ALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQ 242

Query: 241 RWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVW----NSQSELKTEEWDD-- 300
           RW+  +LL+HPFL+D       +  +  ++SP+++LDQ  W    +S+S L + + +D  
Sbjct: 243 RWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHLVSIDHEDPF 302

Query: 301 -----------DRIRRLS--MFRWEIKWES-DGENWMTIRGR---------------CVD 360
                      DRI +L+   F   + W++ D   W+ +RG                CV+
Sbjct: 303 AEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVRGEKHKETEKRDGDEDVICVE 362

Query: 361 GEDEAESISGQTKSLELFDRTVRFRVSDDVISDLVSLIS 364
               +++I      +   D       SDD+I++  S ++
Sbjct: 363 ATSSSQTIEEVEDWISNQDSLFSEYSSDDIINNFYSNVA 399

[Close]

BLAST of Carg27544 vs. TAIR 10
Match: AT1G07150.1 (mitogen-activated protein kinase kinase kinase 13 )

HSP 1 Score: 221.9 bits (564), Expect = 8.6e-58
Identity = 144/357 (40.34%), Postives = 196/357 (54.90%), Query Frame = 0


Query: 3   WIRLAAVGHGSSATVYLASAAPAGDVFAVKSAEL-----PESEFLKTEQRILSSLS-HPS 62
           W+R A +G G    V  A +   G+VFAVKS +L      +SE L+ E  +  SL  HP 
Sbjct: 23  WVRGACIGRGCFGAVSTAISKTNGEVFAVKSVDLATSLPTQSESLENEISVFRSLKPHPY 82

Query: 63  IVSYRGFDVTRENGKLMYNLFMEYATGGTLADEILRCGGWIK-ETAAGFYTREIVKGLEY 122
           IV + G  V++E      NL++EY   G +A    R GG I+ ET    YT  +V  L +
Sbjct: 83  IVKFLGDGVSKEGTTTFRNLYLEYLPNGDVASH--RAGGKIEDETLLQRYTACLVSALRH 142

Query: 123 LHKRGLVHCDIKAKNILIGEDGL-KIADFGCSKWVNEPAAI---GGTPMFMAPEVARGEK 182
           +H +G VHCD+KA+NIL+ +  + K+ADFG +  ++ P A+    G+P++MAPEV R E 
Sbjct: 143 VHSQGFVHCDVKARNILVSQSSMVKLADFGSAFRIHTPRALITPRGSPLWMAPEVIRREY 202

Query: 183 QGISSDIWSLGCTLIEMVNGAPPWPTDGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLGK 242
           QG  SD+WSLGCT+IEM  G P W   G  +  L RI +S E P  PS LSE  +DFL K
Sbjct: 203 QGPESDVWSLGCTIIEMFTGKPAWEDHG--IDSLSRISFSDELPVFPSKLSEIGRDFLEK 262

Query: 243 CLRRNPTERWSASQLLEHPFLEDTNSEWFEESKELDSNSPTSILDQGVW-NSQSELKTEE 302
           CL+R+P +RWS  QLL+HPFL   ++    ES      SP  +LD   W NS  +L+ EE
Sbjct: 263 CLKRDPNQRWSCDQLLQHPFLSQCHNSSPTES------SPRCVLD---WVNSGFDLEEEE 322

Query: 303 WDDDR----------IRRLSMFRWEIKWESDGENWMTIRGRCVDGEDEAESISGQTK 338
            +  R          I  L+     I WESDG  W+ +R    + E      SG T+
Sbjct: 323 EEVGRSEFEDAAKAIICNLATTGGVI-WESDG--WVEVRCHASEEEGTTMEYSGSTR 363

The following BLAST results are available for this feature:

BLAST of Carg27544 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022856.1	1.6e-210	100.00	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
KAG6589157.1	3.5e-210	99.72	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
XP_023531740.1	1.7e-209	99.45	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe...	[more]
XP_022930728.1	1.5e-208	99.17	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata]	[more]
XP_022988825.1	5.5e-208	98.62	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima]	[more]

BLAST of Carg27544 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80888	1.9e-86	48.09	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	3.3e-86	50.73	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
Q9SND6	3.2e-49	36.92	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q40541	5.1e-47	39.05	Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097...	[more]
Q54R82	4.3e-46	35.22	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]

BLAST of Carg27544 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EW68	7.0e-209	99.17	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]
A0A6J1JKQ2	2.7e-208	98.62	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1GLZ4	2.0e-139	76.12	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A5A7T9T3	1.7e-138	71.24	Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=...	[more]
A0A0A0K6U1	1.7e-138	71.31	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043...	[more]

BLAST of Carg27544 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32510.1	1.4e-87	48.09	mitogen-activated protein kinase kinase kinase 17	[more]
AT1G05100.1	2.3e-87	50.73	mitogen-activated protein kinase kinase kinase 18	[more]
AT5G55090.1	1.5e-86	46.56	mitogen-activated protein kinase kinase kinase 15	[more]
AT4G26890.1	2.1e-80	40.60	mitogen-activated protein kinase kinase kinase 16	[more]
AT1G07150.1	8.6e-58	40.34	mitogen-activated protein kinase kinase kinase 13	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..252 e-value: 2.0E-72 score: 256.6
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 6..252 e-value: 1.6E-56 score: 191.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 3..252 score: 43.325668
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 3..307 e-value: 2.4E-69 score: 236.0
None	No IPR available	PANTHER	PTHR48011:SF6	MAP KINASE KINASE KINASE	coord: 1..336
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..336
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 3..252 e-value: 8.57119E-125 score: 357.601
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..32
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 121..133
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 5..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27544-RA	Carg27544-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity