Carg27534 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27534
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	Carg_Chr20: 4324467 .. 4328431 (+)
RNA-Seq Expression	Carg27534
Synteny	Carg27534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATACAGAGACCTCTCTCTATCTCTCTCTGCAATTCTGCGTTCATTTCATGTCCGGTTGATATTTTTCGTTCTGTTGTTAATCATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGGTCTCTTTCTCTCCTCCCTTTTTCCTTTTCTCTGTTCACTCATTTTCTTTTCTCAATTCCTTTCGTTTTTTGTTTCTGTTTTGCAGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTCTCCACTGCCGCACTACTTCGCTCAACTACAATCGGCAAAGTCCGACTCTACGGCGCCGATCCACAGATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGTAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCCTGATTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGATTCAATCCAGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGGTCAGTCAACCGCTCCAATCAAGTTCTCCTAATTTCATTTCAATTTCTTAATCTTAATTAATTAATTAATTAATTAATTCCAATTCTTTCACCTTAAACACCGACGACGTTTACTTTAAGTCAACAGTCAATAGCCTCAAATTGTGACACGTGTCAATAAGCTTTAAATTTATTATTATTTTTTTTTTACCTTTTTTAAGTAAATATGTAATTTTTTTTTTAAGCATATAATTTGAAGATTAATTTACAAAAAGCTTTGGCTAAAAATATTAACGTCACTTTTTTTTGGGGGTTGCTGATGGTTATATTTTATTCTTTTGGGGTTAAAAAATCTCTTTCCATATGCATCGATATTAGCAAATTTATCATTTTCAGAATCAATTTTTTAGAAAGTAATCTGAATGACGTTACTTATCAATTTGGGTTTTTTTTTTTCCTGGCTGCTAGATTTGGAATTTTAATACTTTTTATAGATCTTGTTGCTGTTTATAGTTCTTGTGGTGTATTTCGATTTGGTGCTTGCTTGTGGGTAGTTCGCGTAAATTGCTTGAAACTAAACAATGGGTGTAGATAATGATGTGTTTGAAGTTTTGATTGTTTCTGCCTGCAAGGTGTTTGTGATATTGCGTGAAAGAAATTGATGTGTAAAATCACTCGCTTGCTTGAACGCCCCTTCCTGATTTTACGCCCAATTGAAGATTAAAAGCCTAAATTCTGTTTTATTTTCAACCCAAAAGGCCAATTTAGAAGAAAAAAGTGATCAGGTGGGAAATGTTTTGATTGTTTCTGTCTGCAAGGTGTTTGTGATATTGCGTGAAAGAAATTGATGTATGAAATCAGTAATCACTCGCTTGCTTAAACGCCCCTTCCTGATTTTACACCCAATTGAAGACTAAAAGCCTGATATTCTGTTGTATTTTCAACCCAAAAGGCCAAAAGAATTTAGAAAAAAGTGATGAGGTGGGAGATGTCATTCAACTTTCGAATCTTAGCAAATAAGCTTGCTTTTCCTGTGTGATGTGACCTATATTATGCTCGGTTACAGCTGGCCTGTGCCAATTAATGTCGGTAAGTCCTTCCAAGATATCAATTATTATAGCAGATGAGGAATGCTTACATATTATTTATTGGCCTGGAATTTTTCTTCCTTTTCTTTTTGGGTTTATTGAGCTGTGATATTAGTAAGAGCTAGTGTCCTCGTGACAAGGATTAGAGACGTTTGAAGACACATTTCAAAATTTTGTATTCAAGCCTAGCCCCTCCATGAATGGATGGAGCAGATTAGAGATAACCCTACAATTGGTTAAAAAACGCATAATATCCCAATAAAAAGGAAAAAGAAGGTTTTCTCTCCCTCTCCTCTTCTCCCCTGACTCGTCCTTTTTTTTTTCCCCCCTAATATGCCCGTTAGTCAGAAAGACAACCAAGTGGGCTATAGTGATGTAAGAATTTGGTTTTTCTACTTTCTCCTTTTTGTTGAGAAAAGATATCGGGGAGACCCTTGACTTGGATGGACTCCTGCGCCCTTTTTATTTAGTTTGTTTATCTTGGTGTCCCCTCTCGAGTCAGGTTCGCAGTTAGCTGGACACTATTTTGAATAATTGTGTATTCAAGCCCTCGAAAGAAAATTTTTGGAACTTGAGAACTATTGGTTTTGGTGTGGGTGCTTATAGAAAAAAAATTATAATTATCAAGGGAGCACAGCATATGTGTGGTGCGCTGGAATCCCCCCAGCACAACCAAACAAGCACATTCTGAATTTTGTGGCATTTCTCTCTGTGGTCTTCAACTACCATGTTTGTTTAAAACTTGTATTTATGATATCTTGCAACAGATTCTAATAATTTGAACTACTACATCCATTATTGGGAGCTATTATTATTTGGTCCATGACTCGTATTGATTTCAACAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCTGAAACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCAGATCTAACCATGACATATGATGTTGGGCTCTCAAAGAACAGCCAGGTCAGTCAATGGTTGAATCATTGATTGCTAATTGCATCAACTTTACTTTGATGAACTTTATCCAATCATTTTACATCCATGAAGTTGACTCTTGAATGTTACAGACTCCTACAACTCCAACAACTCCAGCCACCCCGTCCCCAAAACCGACTGCGACTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTACGCTTGCGGGCACGGTATCGATTGTGGGGCGATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTAAGCTTTCTGTTCTAATATCTCTTCCATTCAATTTCAGCCAAGCAATAAGGGAAGGACAGAAAGTTTTCATATTTTGGTGTTATTTTTTTAGTTGAAGTTCTCACATTTGTGGATTTTCAGGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGAGAAGGGAGATCATAGTTCAGTGATATGAGATAAAGAATTAGTTTTCCATATTCTAAGGTGTCTTAAGACATTTGAAAACAAACGAATGAAGCAATAATCTAATTAATTGGCCAACAATTGGTCATTCTTAGCTGCCCTGTCCAACATTCTACAAAGCCAAAATGGCTACCTTCTTAAATTCTATTAATTAAGAATAACAATTTTTTTAGGGGAAAAATCAATCCATGCCCTTTTATATATTAATTTCAACTTCAACTTTTACCTTTTGT

mRNA sequence

GAAATACAGAGACCTCTCTCTATCTCTCTCTGCAATTCTGCGTTCATTTCATGTCCGGTTGATATTTTTCGTTCTGTTGTTAATCATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTCTCCACTGCCGCACTACTTCGCTCAACTACAATCGGCAAAGTCCGACTCTACGGCGCCGATCCACAGATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGTAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCCTGATTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGATTCAATCCAGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCTGAAACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCAGATCTAACCATGACATATGATGTTGGGCTCTCAAAGAACAGCCAGACTCCTACAACTCCAACAACTCCAGCCACCCCGTCCCCAAAACCGACTGCGACTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTACGCTTGCGGGCACGGTATCGATTGTGGGGCGATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGAGAAGGGAGATCATAGTTCAGTGATATGAGATAAAGAATTAGTTTTCCATATTCTAAGGTGTCTTAAGACATTTGAAAACAAACGAATGAAGCAATAATCTAATTAATTGGCCAACAATTGGTCATTCTTAGCTGCCCTGTCCAACATTCTACAAAGCCAAAATGGCTACCTTCTTAAATTCTATTAATTAAGAATAACAATTTTTTTAGGGGAAAAATCAATCCATGCCCTTTTATATATTAATTTCAACTTCAACTTTTACCTTTTGT

Coding sequence (CDS)

ATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTCTCCACTGCCGCACTACTTCGCTCAACTACAATCGGCAAAGTCCGACTCTACGGCGCCGATCCACAGATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGTAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCCTGATTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGATTCAATCCAGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCTGAAACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCAGATCTAACCATGACATATGATGTTGGGCTCTCAAAGAACAGCCAGACTCCTACAACTCCAACAACTCCAGCCACCCCGTCCCCAAAACCGACTGCGACTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTACGCTTGCGGGCACGGTATCGATTGTGGGGCGATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGA

Protein sequence

MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS

Homology

[Close]

BLAST of Carg27534 vs. NCBI nr
Match: KAG6571123.1 (Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010935.1 Glucan endo-1,3-beta-glucosidase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 917.5 bits (2370), Expect = 4.5e-263
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

[Close]

BLAST of Carg27534 vs. NCBI nr
Match: XP_022943626.1 (glucan endo-1,3-beta-D-glucosidase [Cucurbita moschata])

HSP 1 Score: 913.3 bits (2359), Expect = 8.4e-262
Identity = 459/461 (99.57%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           PATPSPKPTA AWCMPKAGASDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTAAAWCMPKAGASDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

[Close]

BLAST of Carg27534 vs. NCBI nr
Match: XP_023512642.1 (glucan endo-1,3-beta-D-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.5 bits (2331), Expect = 1.5e-258
Identity = 455/461 (98.70%), Postives = 456/461 (98.92%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPT 
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPT- 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
             TPSPKPTA AWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 --TPSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458

[Close]

BLAST of Carg27534 vs. NCBI nr
Match: XP_022986522.1 (glucan endo-1,3-beta-D-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 899.8 bits (2324), Expect = 9.7e-258
Identity = 451/461 (97.83%), Postives = 453/461 (98.26%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MA+SSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MASSSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALN LGDFKDVEILVAETGWAY GDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNVLGDFKDVEILVAETGWAYHGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           P+TPSPKPT   WCMPKAG SDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PSTPSPKPTVAVWCMPKAGVSDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY GGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYTGGSS 461

[Close]

BLAST of Carg27534 vs. NCBI nr
Match: XP_038900992.1 (glucan endo-1,3-beta-D-glucosidase-like [Benincasa hispida])

HSP 1 Score: 832.8 bits (2150), Expect = 1.5e-237
Identity = 415/458 (90.61%), Postives = 432/458 (94.32%), Query Frame = 0


Query: 4   SSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGAD 63
           +SF L F+SF LFAA YLADSQSFIGVNYGQVA+NLPPP STA LLRST+I K+RLYGAD
Sbjct: 2   ASFLLPFLSFFLFAAVYLADSQSFIGVNYGQVADNLPPPSSTANLLRSTSIAKIRLYGAD 61

Query: 64  PQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVI 123
           P IIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNV PYYPASNIVL+TVGNEV+
Sbjct: 62  PLIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVNPYYPASNIVLVTVGNEVM 121

Query: 124 SSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQM 183
           SSMDQ LISQL+PAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPV EQ 
Sbjct: 122 SSMDQNLISQLVPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVFEQT 181

Query: 184 MKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDA 243
           MKAVVEF KE  SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG+GIKYMNMFDA
Sbjct: 182 MKAVVEFLKEKKSPFAINPYPFFAYQSDPRPETLAFCLFQPNSGRVDSGSGIKYMNMFDA 241

Query: 244 QLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGT 303
           QLDAVRSALNALGDFKDVEI+VAETGWAYRGDSNEVG ++ENARAYNGNLIAHLRSMVGT
Sbjct: 242 QLDAVRSALNALGDFKDVEIMVAETGWAYRGDSNEVGPSVENARAYNGNLIAHLRSMVGT 301

Query: 304 PLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPAT 363
           PLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPAT
Sbjct: 302 PLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPAT 361

Query: 364 PSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMN 423
           PSP+PTA AWC+PKAG  D QLQ NLDYACGHGIDCG IQPGGACFEPNTV SHAAYAMN
Sbjct: 362 PSPEPTAPAWCLPKAGIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVHSHAAYAMN 421

Query: 424 LYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           LYYQSLGKNPW+CDFSQTA LTSTNPSYN C+YPGGS+
Sbjct: 422 LYYQSLGKNPWNCDFSQTAILTSTNPSYNACTYPGGST 459

[Close]

BLAST of Carg27534 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.9e-180
Identity = 313/455 (68.79%), Postives = 380/455 (83.52%), Query Frame = 0


Query: 7   SLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQI 66
           SL F+ F L   FY A+SQSF+GVNYGQ+++NLP   +T  LL+STTI KVRL+GA+P +
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAV 68

Query: 67  IKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSM 126
           IKA ANTG+ IVIG  NGDIP LASNPN A+Q++ +NV+ +YPASNI+ ITVGNEV++S 
Sbjct: 69  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSG 128

Query: 127 DQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKA 186
           DQ LISQLLPAMQNVQNALN+A+LGGKVKVSTVH+MAVLSQS PPSSG FNP L   MKA
Sbjct: 129 DQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKA 188

Query: 187 VVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLD 246
           +++F+  N +PF I+PYP+FAY++ P  +TLAFCLFQPN+G+VDSG G KY NMFDAQ+D
Sbjct: 189 LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVD 248

Query: 247 AVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLM 306
           AV SALNA+G FKD+EI+VAETGW + GDSNEVG +++NA+AY GNLI HL+S VGTPLM
Sbjct: 249 AVHSALNAMG-FKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLM 308

Query: 307 PGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATPSP 366
           PGKS+DTY+F+LYDE+ K G +SE+ FGLF+PD + TYDVGL KN+Q PTTP TP TP+P
Sbjct: 309 PGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATP-TPTP 368

Query: 367 KPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYY 426
           K  A +WC+PK G SD QL  N++YACG GIDCG IQPGGACFEPNTVK+HAAY MNLYY
Sbjct: 369 K-AAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYY 428

Query: 427 QSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           QS G+N W+CDFSQTATLT+TNPSY  C++P GS+
Sbjct: 429 QSAGRNSWNCDFSQTATLTNTNPSYGACNFPSGSN 460

[Close]

BLAST of Carg27534 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 4.9e-180
Identity = 313/457 (68.49%), Postives = 377/457 (82.49%), Query Frame = 0


Query: 3   ASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGA 62
           A S S+ F+   L + F  + ++ FIGVNYGQVA+NLPPP  T  LL+ST+I KVRLYGA
Sbjct: 2   ALSISIYFLLIFL-SHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 61

Query: 63  DPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEV 122
           DP IIKALA TG+GIVIG ANGD+P+LAS+PN+A QWIN+NV+P+YPAS I+LITVGNE+
Sbjct: 62  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 121

Query: 123 ISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQ 182
           + S D  L++QLLPAMQNVQ AL + +LGGK+KVSTV+SM VL  SDPPSSG F    + 
Sbjct: 122 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQT 181

Query: 183 MMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFD 242
            +K +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLF+PN+GRVDS TGIKY NMFD
Sbjct: 182 GLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 241

Query: 243 AQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVG 302
           AQ+DAV SAL ++G F+ VEI+VAETGWA RGD+NEVG +++NA+AYNGNLIAHLRSMVG
Sbjct: 242 AQVDAVHSALKSMG-FEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 301

Query: 303 TPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPA 362
           TPLMPGK VDTYIFALYDENLKPGP+SER+FGLF+ DL+M YDVGL+K+S      ++  
Sbjct: 302 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS------SSSQ 361

Query: 363 TPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAM 422
           TPS K T++ WC+PK GA++ +LQ +LD+ACGHGIDCGAIQPGGACFEPN V SHAAYAM
Sbjct: 362 TPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAM 421

Query: 423 NLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGG 460
           N+Y+Q   K P DCDFS+TAT+TS NPSYN C YPGG
Sbjct: 422 NMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

[Close]

BLAST of Carg27534 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.7e-100
Identity = 196/441 (44.44%), Postives = 277/441 (62.81%), Query Frame = 0


Query: 28  IGVNYGQVANNLPPPVSTAALLRS-TTIGKVRLYGADPQIIKALANTGIGIVIGVANGDI 87
           IGVNYG  A+NLP P S A  L + TTI +V+L+ A+P  I A A T I + + + N  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 88  PALASNP---NSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLISQLLPAMQNVQN 147
           PALA      ++A  WI  N+ PY PA+N+ L+  GNE++ S D  LI  LLPAM+ +  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 148 ALNSANLGGKVKVSTVHSMAVLSQSDP-PSSGRFNPVLE-QMMKAVVEFEKENASPFAIN 207
           AL +  L G V+V+T H + +L+ SD  PS+  F      ++  A+++F ++  SPF +N
Sbjct: 146 ALKAEGLTG-VRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 208 PYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDV 267
           PYP+F+Y    R ETL + LF+PNSG  D  T + Y +M DAQ+DA+ +A+  LG + DV
Sbjct: 206 PYPYFSY----RPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLG-YGDV 265

Query: 268 EILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDE 327
           +I V E GW  + +  ++G  ++ AR +N  +I    S  GTPLMP ++ +TY+F+L+DE
Sbjct: 266 DIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDE 325

Query: 328 NLKPGPTSERSFGLFRPDLTMTYDVGLSKN--SQTPT-TPTTPATPSPKPTATA---WCM 387
           N KPGP +ER FGLF PD T  YD+GL ++  S  PT +P     PSPKP  +    WC+
Sbjct: 326 NQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNPSPKPAPSGGGKWCV 385

Query: 388 PKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWD 447
            K GA+   LQ N++YACG  +DC  IQ GGACF PN++++HA+Y MN YYQ+ G     
Sbjct: 386 AKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLA 445

Query: 448 CDFSQTATLTSTNPSYNGCSY 457
           CDF  T  +TS++PSY GC Y
Sbjct: 446 CDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of Carg27534 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 5.8e-96
Identity = 195/469 (41.58%), Postives = 278/469 (59.28%), Query Frame = 0


Query: 10  FVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKA 69
           FVS   F  F +A   S IG+ YG+ A+NLP P   + L++   I  VR+Y A+  ++KA
Sbjct: 12  FVSILAFLNFGMA---SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKA 71

Query: 70  LANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQG 129
            ANTGI ++IGV N D+ A A   ++   W++ N++PYYP++ I  I+VG EV  + D  
Sbjct: 72  FANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA 131

Query: 130 LISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVE 189
               +LPAM+N+  AL  + L  K+K+S+ HS+A+LS+S PPSS  F+      +K ++E
Sbjct: 132 -TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLE 191

Query: 190 FEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVR 249
           F  EN SPF I+ YP++AY+       L + LF+ +S  VD  TG+ Y NMFDAQLDA+ 
Sbjct: 192 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 251

Query: 250 SALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGK 309
            AL A+  FK V+++V E+GW  +G   E   T ENA AYN NLI H+    GTP  PG+
Sbjct: 252 FALTAM-SFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGE 311

Query: 310 SVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPAT-PSPKP 369
            +D Y+F+L++EN KPG  SER++G+F  + T  Y +  +  + TP +PT   T  SP P
Sbjct: 312 EIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSP 371

Query: 370 TAT--------------------AWCMPKAGASDAQLQLNLDYACGHG-IDCGAIQPGGA 429
           +++                     WC+  + AS  +LQ  LD+ACG G +DC A+QP   
Sbjct: 372 SSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQP 431

Query: 430 CFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY 457
           CFEP+TV SHA+YA N YYQ  G +  DC F+  +     +PSY  C Y
Sbjct: 432 CFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Carg27534 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.1e-86
Identity = 169/431 (39.21%), Postives = 257/431 (59.63%), Query Frame = 0


Query: 28  IGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALANTGIGIVIGVANGDIP 87
           +GV YG+ A++LP P     L++   I  VR+Y  + Q++KA  NT I ++IGV N D+ 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 88  ALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLISQLLPAMQNVQNALNS 147
           A + + ++   W+  +V+PYYP + I  ITVG E          S ++PAMQNV  AL  
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALRK 145

Query: 148 ANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEKENASPFAINPYPFFA 207
             L  ++KVST  S+ +LS+S PPS+G FN      ++ ++EF  EN SPF I+ YP++A
Sbjct: 146 VGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYYA 205

Query: 208 YQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDVEILVAE 267
           Y+  P   +L + LF+ +S  +D  TG+ Y NMFDAQ+DA+  AL AL +F+ ++I+V E
Sbjct: 206 YRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTAL-NFRTIKIMVTE 265

Query: 268 TGWAYRGDSNE-VGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDENLKPG 327
           TGW  +G   E    + +NA  YN N+I H+ +  GTP  PG++++ YIF+L++EN K G
Sbjct: 266 TGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRKAG 325

Query: 328 PTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATPSPKPTATAWCMPKAGASDAQLQ 387
             SER++GLF PD T  Y +  +  S    + ++    S   ++ +WC+  + AS+  L+
Sbjct: 326 LDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSS--GSSNSWCIASSKASERDLK 385

Query: 388 LNLDYACGHG-IDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLT 447
             LD+ACG G +DC AIQP   CF+P+T+ SHA++  N Y+Q        C F       
Sbjct: 386 GALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKV 445

Query: 448 STNPSYNGCSY 457
           + +PSY+ C Y
Sbjct: 446 NKDPSYDKCIY 453

[Close]

BLAST of Carg27534 vs. ExPASy TrEMBL
Match: A0A6J1FS76 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448341 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.1e-262
Identity = 459/461 (99.57%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           PATPSPKPTA AWCMPKAGASDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTAAAWCMPKAGASDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

[Close]

BLAST of Carg27534 vs. ExPASy TrEMBL
Match: A0A6J1J7S0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484236 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.7e-258
Identity = 451/461 (97.83%), Postives = 453/461 (98.26%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MA+SSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MASSSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALN LGDFKDVEILVAETGWAY GDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNVLGDFKDVEILVAETGWAYHGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           P+TPSPKPT   WCMPKAG SDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PSTPSPKPTVAVWCMPKAGVSDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY GGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYTGGSS 461

[Close]

BLAST of Carg27534 vs. ExPASy TrEMBL
Match: A0A1S3C673 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497492 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.5e-229
Identity = 399/463 (86.18%), Postives = 427/463 (92.22%), Query Frame = 0


Query: 4   SSFSLTFVSFSLFAA-----FYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVR 63
           +SFSL F S  LFAA     FY+ADSQSFIGVNYGQ+++NLPPP STA LLRST+I K+R
Sbjct: 2   ASFSLPFFSLFLFAAASSTVFYIADSQSFIGVNYGQLSDNLPPPSSTANLLRSTSISKIR 61

Query: 64  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 123
           LY ADP IIK+LAN+G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITV
Sbjct: 62  LYNADPLIIKSLANSGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITV 121

Query: 124 GNEVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 183
           GNEV+SSMDQ LISQL+PAM+NVQNA+N+A+LGGKVKVSTVHSMAVLSQSDPPSSGR NP
Sbjct: 122 GNEVMSSMDQNLISQLVPAMRNVQNAVNAASLGGKVKVSTVHSMAVLSQSDPPSSGRINP 181

Query: 184 VLEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYM 243
           + E  MKAVVEF KEN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYM
Sbjct: 182 MFETTMKAVVEFLKENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYM 241

Query: 244 NMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLR 303
           NMFDAQLDAVRS LNALGDFKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLR
Sbjct: 242 NMFDAQLDAVRSGLNALGDFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLR 301

Query: 304 SMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTP 363
           SMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLF P+LTMTYDVGLSKNSQTPTTP
Sbjct: 302 SMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFYPNLTMTYDVGLSKNSQTPTTP 361

Query: 364 TTPATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHA 423
           TTP TPSPKPTA  WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNTVKSHA
Sbjct: 362 TTPTTPSPKPTAPVWCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVKSHA 421

Query: 424 AYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AYAMNLYYQSLGKNPW+CDFSQTATLTSTNPSYN C+YPGGS+
Sbjct: 422 AYAMNLYYQSLGKNPWNCDFSQTATLTSTNPSYNACTYPGGST 464

[Close]

BLAST of Carg27534 vs. ExPASy TrEMBL
Match: A0A0A0LL52 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G246520 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 394/463 (85.10%), Postives = 425/463 (91.79%), Query Frame = 0


Query: 4   SSFSLTFVSFSLFAA-----FYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVR 63
           +SFSL F SF LFAA     FY+ADSQSFIGVNYGQ+++NLPPP +TA LLRST+I K+R
Sbjct: 2   ASFSLPFFSFFLFAAASSAVFYIADSQSFIGVNYGQLSDNLPPPSATANLLRSTSISKIR 61

Query: 64  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 123
           LY ADP IIK+LAN+G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITV
Sbjct: 62  LYNADPLIIKSLANSGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITV 121

Query: 124 GNEVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 183
           GNEV+SSMDQ LISQL+PAM+NVQNA+N+ANLGGKVKVSTVHSMAVLSQSDPPSSGR NP
Sbjct: 122 GNEVMSSMDQNLISQLVPAMRNVQNAVNAANLGGKVKVSTVHSMAVLSQSDPPSSGRINP 181

Query: 184 VLEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYM 243
           + E  MK VVEF KEN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYM
Sbjct: 182 MFEGTMKEVVEFLKENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYM 241

Query: 244 NMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLR 303
           NMFDAQLDAVRSALNALG FKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLR
Sbjct: 242 NMFDAQLDAVRSALNALGGFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLR 301

Query: 304 SMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTP 363
           SMVGTPLMPGKSVDTYIFALYDENLKPGPTSER+FGLF P+LTMTYDVGLSKNSQTPTTP
Sbjct: 302 SMVGTPLMPGKSVDTYIFALYDENLKPGPTSERAFGLFYPNLTMTYDVGLSKNSQTPTTP 361

Query: 364 TTPATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHA 423
           TTP TPS KPTA  WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNT++SHA
Sbjct: 362 TTPTTPSSKPTAPVWCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTIQSHA 421

Query: 424 AYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           AYAMNLYYQSLGKNPW+CDFSQTATLTS NPSYN C+YPGGS+
Sbjct: 422 AYAMNLYYQSLGKNPWNCDFSQTATLTSANPSYNACTYPGGST 464

[Close]

BLAST of Carg27534 vs. ExPASy TrEMBL
Match: A0A5D3CQ55 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001950 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.2e-226
Identity = 389/449 (86.64%), Postives = 417/449 (92.87%), Query Frame = 0


Query: 13  FSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALAN 72
           F+ F   + ADSQSFIGVNYGQ+++NLPPP STA LLRST+I K+RLY ADP IIK+LAN
Sbjct: 47  FTPFFFLFFADSQSFIGVNYGQLSDNLPPPSSTANLLRSTSISKIRLYNADPLIIKSLAN 106

Query: 73  TGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLIS 132
           +G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITVGNEV+SSMDQ LIS
Sbjct: 107 SGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITVGNEVMSSMDQNLIS 166

Query: 133 QLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEK 192
           QL+PAM+NVQNA+N+A+LGGKVKVSTVHSMAVLSQSDPPSSGR NP+ E  MKAVVEF K
Sbjct: 167 QLVPAMRNVQNAVNAASLGGKVKVSTVHSMAVLSQSDPPSSGRINPMFETTMKAVVEFLK 226

Query: 193 ENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSAL 252
           EN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYMNMFDAQLDAVRS L
Sbjct: 227 ENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYMNMFDAQLDAVRSGL 286

Query: 253 NALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVD 312
           NALGDFKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLRSMVGTPLMPGKSVD
Sbjct: 287 NALGDFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLRSMVGTPLMPGKSVD 346

Query: 313 TYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATPSPKPTATA 372
           TYIFALYDENLKPGPTSERSFGLF P+LTMTYDVGLSKNSQTPTTPTTP TPSPKPTA  
Sbjct: 347 TYIFALYDENLKPGPTSERSFGLFYPNLTMTYDVGLSKNSQTPTTPTTPTTPSPKPTAPV 406

Query: 373 WCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKN 432
           WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNTVKSHAAYAMNLYYQSLGKN
Sbjct: 407 WCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVKSHAAYAMNLYYQSLGKN 466

Query: 433 PWDCDFSQTATLTSTNPSYNGCSYPGGSS 462
           PW+CDFSQTATLTSTNPSYN C+YPGGS+
Sbjct: 467 PWNCDFSQTATLTSTNPSYNACTYPGGST 495

[Close]

BLAST of Carg27534 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 632.1 bits (1629), Expect = 3.5e-181
Identity = 313/457 (68.49%), Postives = 377/457 (82.49%), Query Frame = 0


Query: 3   ASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGA 62
           A S S+ F+   L + F  + ++ FIGVNYGQVA+NLPPP  T  LL+ST+I KVRLYGA
Sbjct: 2   ALSISIYFLLIFL-SHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 61

Query: 63  DPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEV 122
           DP IIKALA TG+GIVIG ANGD+P+LAS+PN+A QWIN+NV+P+YPAS I+LITVGNE+
Sbjct: 62  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 121

Query: 123 ISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQ 182
           + S D  L++QLLPAMQNVQ AL + +LGGK+KVSTV+SM VL  SDPPSSG F    + 
Sbjct: 122 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQT 181

Query: 183 MMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFD 242
            +K +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLF+PN+GRVDS TGIKY NMFD
Sbjct: 182 GLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 241

Query: 243 AQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVG 302
           AQ+DAV SAL ++G F+ VEI+VAETGWA RGD+NEVG +++NA+AYNGNLIAHLRSMVG
Sbjct: 242 AQVDAVHSALKSMG-FEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 301

Query: 303 TPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPA 362
           TPLMPGK VDTYIFALYDENLKPGP+SER+FGLF+ DL+M YDVGL+K+S      ++  
Sbjct: 302 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS------SSSQ 361

Query: 363 TPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAM 422
           TPS K T++ WC+PK GA++ +LQ +LD+ACGHGIDCGAIQPGGACFEPN V SHAAYAM
Sbjct: 362 TPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAM 421

Query: 423 NLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGG 460
           N+Y+Q   K P DCDFS+TAT+TS NPSYN C YPGG
Sbjct: 422 NMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

[Close]

BLAST of Carg27534 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.6e-171
Identity = 296/452 (65.49%), Postives = 361/452 (79.87%), Query Frame = 0


Query: 6   FSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQ 65
           F L F+ FS+      +++QSFIGVNYG +++NLPPP  TA LL+ST+I KVRLY AD  
Sbjct: 7   FLLLFILFSISP----SNAQSFIGVNYGLLSDNLPPPSQTAKLLQSTSIQKVRLYNADSS 66

Query: 66  IIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISS 125
           II +L  TGIGIVIGVANGD+P++AS+ N A+QWIN+NV+P+YPASNI+LI VGNEV+ S
Sbjct: 67  IITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLS 126

Query: 126 MDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMK 185
            D  L++QLLPAMQNVQ AL + +LGGK+KVSTVH+M VL  S+PPS+G F P  +  +K
Sbjct: 127 NDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAGLK 186

Query: 186 AVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQL 245
            +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLFQPN GRVDS TGIKYMNMFDAQ+
Sbjct: 187 GILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQV 246

Query: 246 DAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPL 305
           DAV SAL ++G F+ VE+LVAETGW   GDSNEVG ++ENA+AYNGNLIAHLRSMVGTPL
Sbjct: 247 DAVHSALKSIG-FEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPL 306

Query: 306 MPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATPS 365
           MPGKS+DTYIFAL+DENLKPGP+ E+SFGLF+PDL+M YD+GL+K   T ++ T+ +   
Sbjct: 307 MPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTK---TTSSQTSQSPQL 366

Query: 366 PKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLY 425
            K T+  WC+PK  A+  QLQ +LD+ CG GIDCG I PGG CFEPN V SH AYAMNLY
Sbjct: 367 GKVTSMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNNVASHTAYAMNLY 426

Query: 426 YQSLGKNPWDCDFSQTATLTSTNPSYNGCSYP 458
           +Q   +NP DCDFS+TA +TS NPSY+ C YP
Sbjct: 427 FQKSPENPTDCDFSKTARITSENPSYSSCVYP 450

[Close]

BLAST of Carg27534 vs. TAIR 10
Match: AT5G42720.1 (Glycosyl hydrolase family 17 protein )

HSP 1 Score: 465.7 bits (1197), Expect = 4.3e-131
Identity = 241/374 (64.44%), Postives = 289/374 (77.27%), Query Frame = 0


Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           M AS +SL  + FS     +L+ SQ F+GVNYG  A+NLPPP ++A LL+STT  KVRLY
Sbjct: 1   MRASVYSLILLFFS--CLLHLSKSQPFLGVNYGLTADNLPPPSASAKLLQSTTFQKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           G+DP +IKALANTGI IVIG +NGD+P LAS+P+ A  W+ TNVVPYYPAS IVLI VGN
Sbjct: 61  GSDPAVIKALANTGIEIVIGASNGDVPGLASDPSFARSWVETNVVPYYPASKIVLIAVGN 120

Query: 121 EVISSMDQGLISQLLPAMQNVQNALNSANL-GGKVKVSTVHSMAVLSQSDPPSSGRFNPV 180
           E+ S  D  L+SQLLPAM+NVQ AL +A+L GGK+KVSTVH M+VL+ SDPPS+  F P 
Sbjct: 121 EITSFGDNSLMSQLLPAMKNVQTALEAASLGGGKIKVSTVHIMSVLAGSDPPSTAVFKPE 180

Query: 181 LEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMN 240
              ++K ++EF  E  SPFA+NPYPFFAYQ D R ETLA+CLFQ N GRVD  + +KYMN
Sbjct: 181 HADILKGLLEFNSETGSPFAVNPYPFFAYQDDRRPETLAYCLFQANPGRVDPNSNLKYMN 240

Query: 241 MFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRS 300
           MFDAQ+DAV SALN++G FKDVEI+VAETGW Y+GD  E G T+ENARAYN NLIAHL+S
Sbjct: 241 MFDAQVDAVYSALNSMG-FKDVEIMVAETGWPYKGDPEEAGATVENARAYNKNLIAHLKS 300

Query: 301 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKN---SQTPT 360
             GTPLMPG+ +DTY+FALYDENLKPG  SER+FGLFRPDLTMTYD+GL+K    +QT  
Sbjct: 301 GSGTPLMPGRVIDTYLFALYDENLKPGKGSERAFGLFRPDLTMTYDIGLTKTTNYNQTSM 360

Query: 361 TPTTPATPSPKPTA 371
            P +P  P   P A
Sbjct: 361 APLSPTRPRLPPAA 371

[Close]

BLAST of Carg27534 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-118
Identity = 220/460 (47.83%), Postives = 305/460 (66.30%), Query Frame = 0


Query: 5   SFSLTFVSFSLFA-----AFYLADSQSFIGVNYGQVANNLPPPVSTAA-LLRSTTIGKVR 64
           ++ L F S S+F            S++ IGVNYG +ANNLPPP   A  LL ST I ++R
Sbjct: 2   NYVLPFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIR 61

Query: 65  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 124
           L+  DPQI++A ANTGI + + V N  IP L +N +SA QWI+ ++ P++P++NI+ I V
Sbjct: 62  LFDTDPQILQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILV 121

Query: 125 GNEVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 184
           GNEVIS+ D  LI  L+PAMQ++  AL SA+L  ++++ST HS+  L+ S PPSS +F  
Sbjct: 122 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 181

Query: 185 VLE-QMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKY 244
             + Q++K ++ F +  +SPF +NPYPFF Y      ETL F LF+PN G  D  T + Y
Sbjct: 182 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYS----IETLDFALFRPNPGLFDQHTKLLY 241

Query: 245 MNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHL 304
            NM DAQLD+V SA++ LG F DVEI++ E GW   GD +++G  ++ A  +N NLIA +
Sbjct: 242 TNMLDAQLDSVYSAMDKLG-FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARV 301

Query: 305 RSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTT 364
            S  GTPLMP ++ +TYIFAL++ENLK GPTSER+FG+FR DLT  YD+G+ + +   + 
Sbjct: 302 DSGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSD 361

Query: 365 PT-TPATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKS 424
           P   P +P    ++  WC+ KAGA    LQ N+DY CG G+DC  I  GG C+ PNTVK+
Sbjct: 362 PVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKA 421

Query: 425 HAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY 457
           H+ YAMNLYYQ++ K+ +DCDF  T  +T+ +PSY  C Y
Sbjct: 422 HSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

[Close]

BLAST of Carg27534 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-116
Identity = 218/456 (47.81%), Postives = 303/456 (66.45%), Query Frame = 0


Query: 5   SFSLTFVSFSLFA-----AFYLADSQSFIGVNYGQVANNLPPPVSTAA-LLRSTTIGKVR 64
           ++ L F S S+F            S++ IGVNYG +ANNLPPP   A  LL ST I ++R
Sbjct: 2   NYVLPFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIR 61

Query: 65  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 124
           L+  DPQI++A ANTGI + + V N  IP L +N +SA QWI+ ++ P++P++NI+ I V
Sbjct: 62  LFDTDPQILQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILV 121

Query: 125 GNEVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 184
           GNEVIS+ D  LI  L+PAMQ++  AL SA+L  ++++ST HS+  L+ S PPSS +F  
Sbjct: 122 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 181

Query: 185 VLE-QMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKY 244
             + Q++K ++ F +  +SPF +NPYPFF Y      ETL F LF+PN G  D  T + Y
Sbjct: 182 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYS----IETLDFALFRPNPGLFDQHTKLLY 241

Query: 245 MNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHL 304
            NM DAQLD+V SA++ LG F DVEI++ E GW   GD +++G  ++ A  +N NLIA +
Sbjct: 242 TNMLDAQLDSVYSAMDKLG-FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARV 301

Query: 305 RSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTT 364
            S  GTPLMP ++ +TYIFAL++ENLK GPTSER+FG+FR DLT  YD+G+ + +   + 
Sbjct: 302 DSGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSD 361

Query: 365 PT-TPATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKS 424
           P   P +P    ++  WC+ KAGA    LQ N+DY CG G+DC  I  GG C+ PNTVK+
Sbjct: 362 PVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKA 421

Query: 425 HAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYN 453
           H+ YAMNLYYQ++ K+ +DCDF  T  +T+ +PS N
Sbjct: 422 HSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSMN 451

The following BLAST results are available for this feature:

BLAST of Carg27534 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571123.1	4.5e-263	100.00	Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022943626.1	8.4e-262	99.57	glucan endo-1,3-beta-D-glucosidase [Cucurbita moschata]	[more]
XP_023512642.1	1.5e-258	98.70	glucan endo-1,3-beta-D-glucosidase [Cucurbita pepo subsp. pepo]	[more]
XP_022986522.1	9.7e-258	97.83	glucan endo-1,3-beta-D-glucosidase-like [Cucurbita maxima]	[more]
XP_038900992.1	1.5e-237	90.61	glucan endo-1,3-beta-D-glucosidase-like [Benincasa hispida]	[more]

BLAST of Carg27534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94G86	2.9e-180	68.79	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q9M069	4.9e-180	68.49	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
P52409	1.7e-100	44.44	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q8VYE5	5.8e-96	41.58	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	4.1e-86	39.21	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of Carg27534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FS76	4.1e-262	99.57	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448341 P...	[more]
A0A6J1J7S0	4.7e-258	97.83	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484236 PE=...	[more]
A0A1S3C673	3.5e-229	86.18	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497492 PE=3 SV...	[more]
A0A0A0LL52	1.2e-226	85.10	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G246520 PE=3...	[more]
A0A5D3CQ55	6.2e-226	86.64	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Carg27534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34480.1	3.5e-181	68.49	O-Glycosyl hydrolases family 17 protein	[more]
AT2G16230.1	5.6e-171	65.49	O-Glycosyl hydrolases family 17 protein	[more]
AT5G42720.1	4.3e-131	64.44	Glycosyl hydrolase family 17 protein	[more]
AT5G24318.1	2.5e-118	47.83	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.2	2.3e-116	47.81	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 372..456 e-value: 9.7E-46 score: 167.9
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 373..443 e-value: 2.4E-21 score: 76.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 369..456 e-value: 3.1E-10 score: 42.3
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 27..355 e-value: 1.3E-123 score: 414.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 348..369
None	No IPR available	PANTHER	PTHR32227:SF227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 7	coord: 5..453
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 28..346 e-value: 6.7E-82 score: 275.6
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 5..453
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 28..347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27534-RA	Carg27534-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds