Carg27498 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27498
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNon-specific serine/threonine protein kinase
LocationCarg_Chr11: 6029583 .. 6033189 (-)
RNA-Seq ExpressionCarg27498
SyntenyCarg27498
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGCTAAAATAATAGAATTTCGAATTGGGGACTAATAGAATTTTGGGTTCTAATCTTTACAAAAACTTCCTCTTTAAACCACCACGCTACCAGCGACAACGAGGACTTGCGGCTTCGTTCGGCTCCTTTTTCCATTTGCAGTTGTATTTCTTTCATTTTCAGTTTGATTTCATATCGATTCTAAGAGTTAATTCGGGTGAGTTTCTCTAACGTATATCATAATTTCGTTTATGGGATTCTTCTGTACTCTGCTTGTTCTTGCCTTCTCTTGATTCCAATTTTCCAAGTGTATGAACTACTGGCTAATTCATTTTTTTCTTGCAAATTTGTTGAGTTTATTAGTAGGTTTATATGTTCACACTATGGGATTCATGTTTAGGGCTCTGGTTGTAGAATTAATAACTTGGAAGTTTCTTCCAGGAACTTCTATTTTGTTATTCAACACATCCTGCTGCTGCCCCTACTGTAGTTATGGAGACAAAGACGGATAGTAATGATGGCTCACTCGTTTTAATTAAGCAAGGAGCTGAAGCTGTAAGTCTGAATCTCTAGTCCATTAATCTGGGATGTTCTTTGGAAAAGGGAGATTGTTCTATAAACAACATTTTTGGAAGAAGTACTAACCAAGTTTTTTAGAACTTTCAGAATTAATTTTCTCTTTGTAAGCAAACATAGTGAAGAATTTGAAAAATGTTTTTCATTGTTCCAAAAGTTATACCAAACTCATCTTACGAATTTTAGGTATTAAGTACACAGGTCTTTTGTTATATTGTTTTTTGTTCTTACCTTGCAGAGAGTGTTTGAGTCTACTTTCGTAGGAAGGCGATCGATTGTTAAGGAACGATTCTCAAAGAAATATAGACATCCATCTCTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGTTTGTTTTCTTCTAATGAAATGCCTTTTTGTAGTTGAAGAAATAACTAGCGAAAAGATTGTCTTGCATATGTTTGACTGAAACTAAATGATATAGGAAGCAAGGTGCATGACCAAAGCTAGGCGACTCGCGGTTTCTACTCCAGTGTTGTATGCTGTTGACCCGATATTACATACCCTGACATTTGAGTATGTGGAAGGTCGTTCTGTGAAAGATATATTACTTGAAATTGGGTCAAGTGGTGATGGTTCAAAACAGTTGAAGGACATTGCAATGCAGATCGGCGTTGCAATTGGGAAGTTGCATGATGGTGGTTTAATTCATGGTGATTTGACGACGTCTAACATGTTGGTCAGGAGTTGTACCAATGAACTTGTGAGTTGAATCTTTGGTTATAATAATTTACAATGTGAGAAATTTTAGTTTGTCGTGCTAAGATTTGTAATAAATTGATCTCTGTCATAAGAGCAATTGTCTTTTCTCAGGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACTATTCCTGAAGATAAAGCAGTTGATCTATATGTGTTGGAACGAGCACTGCTATCGATGCATTCTTCATGTGGCAATGTGGTTAGTTGGTTTCCCTTGCACTTGGTTTGCTGTAAGTTTTTTTTTTTTTTTTTTTTTGTCCTTCCCAATTAGAGATTCTTTTTCTATAAATGTAAACATTTGTGTGGTAAAGACTTATGTTGTTTATTATCCACCTGCGTGTAGCAATAGAATTAGTGATGGATAGGAGGAGATGACAATTAGAGTTCTATCGTGAGATCTCACCTCGGTTGGGGAGGAGAACAAAGTATTCTTTTTAAAGGTGTGGAAACCTCTCCTTAGCAGATGCATTTTAAAAACTTTGAGGGGAAGCCCAAAAAGAAAAGTCTAAAGAGGACAATATTTGCTAGCGGTGGGCTTGGTCTATTACAAATGGTATTAGAGCTAGAAACTGGGTCATGTGCCAGCGAAGAGGTTGAGCCCTAAAGGGAGTGGACACGAGGCGGTGTGCCAGCAAAGACGCTAGGGCGGGGGGGGGGGGGGGGGGTGGGATTGGGAGGTCCCACATCGATTGTAGAAAGGGAACGAGTGCCAACGAGGACGCTGGGCCTCGAAGGGGGGGTGGATTGTGAGATACCACATCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGAAAACCTCTTCTTAGCAGACACGTTTAAGAATTTTGAGAGGAAGCCCGAAAAGGAAAGCCCAAAAAAAACAACATTGGCTAGCGGTGGGTTTGGGCTGCTACATCTATCTAGATAGGAAGTACCCAAAAGATGTAACTATAAATCAAGACCCCTTGCTTCTCTCATGCACGATGTTATCTGCTGAATGACAAAAGAAGTTAACAGACTTCCGGCCTTCTGCTAGCCATATTTTACACCATCCTCCATGCAATTTTACAATTATATCATGCCTTCTGTGCCCATTCCTCTGACTATTCTCTTCATATTTCTCTAATCCTAGAACTATGCAAAAAGAGGGATATTGAGATTCCTCCCAGGATTCTCTCCTGCAAGCTGACATATTCATATTCTGCTAAATGACCAAAATAAATTAACAATTTTCTCTCGTTCTGTTAAGTGTATGTTTCTAACCTTCCCTCTTGCAACTCTACTGTTATCTCAGTGTAGAATTCGTAGCGTAGTTATAAAATGTGGTTCCCTTTCTTAGTCTAACTCAGTCGTGAGGGCTGCAGTTTACTAATGCTGTATTACAATAAACGACCCACAATTTTCTTCTGTTAGTTTGCTTGCTCAATTTGGGGCTGGTTTTTGGAGGCCGATGGGGGTAGTTTAGCCCGTAACTGGGACTGTTGACATACGTTGAAGAGGGTTTTCTTTCACCCTCCCCTCAGACATATGGCCGTTCCTCTAGAGTGCTAGTGTTTATGCAGTGTTATGGGATGGCTTGAGGGAAACAATAAAACCTGTGGAAAAGTGGTCTGGGAGGAGGAGGTATAGTCCCTTGCTAGGTTTGATGCCTCACTTGAGGCATCGGTCTCCGAAGTCTTTTGTTATTACATGTCAGGCTTAGGTTTGTGGCTCTCTTTTTAGGCTATCATTTAGTTGGCCCTCTTGTTGGTTTTTTGTTTTAGTGATTTTCATGCAATCCATATGACTACTTGTTGACAAACTGGGCTGAATTGATACCCAAATTGATATACATTGATGGTTCTTACATATTTTATAGATGGAGCAAATTCTTGCTTCATACCGGAAGACTTCGAAACAGTGGTCGTCAACCTCGAACAAATTAGCTCAAGGTAGTGATCCCATTTTTATCATTCATTATCTGTTTTATTCCCTATGATTTCTTAGTTTACTTCTCTTGCTTTTCACTTTGATTTTACAGTGCGTCAGCGAGGCCGAAAACGCACCATGGTTGGTTAACATCTCTCTTTTTAGTGACCCTTTGTAGTAGACTCATTGAACTTCTTGAACAATGTTTATGTAATTGCAATTCTTCAGGAAAAAACCTTCAGAGTCTTGTATTTAGTTATAGTGAAGTGTCTTTGACCATAAAAGCTATATATTTAAGTGAATCAGACGATTTGTTTTTGTTGTTTTGGAGATTTATAATTAGATGTGGTGAGCTTTTTGCACTTATGAGACTATTCCTGTAAGTTCGACTTTTACAGATTCTG

mRNA sequence

TGGCTAAAATAATAGAATTTCGAATTGGGGACTAATAGAATTTTGGGTTCTAATCTTTACAAAAACTTCCTCTTTAAACCACCACGCTACCAGCGACAACGAGGACTTGCGGCTTCGTTCGGCTCCTTTTTCCATTTGCAGTTGTATTTCTTTCATTTTCAGTTTGATTTCATATCGATTCTAAGAGTTAATTCGGGAACTTCTATTTTGTTATTCAACACATCCTGCTGCTGCCCCTACTGTAGTTATGGAGACAAAGACGGATAGTAATGATGGCTCACTCGTTTTAATTAAGCAAGGAGCTGAAGCTAGAGTGTTTGAGTCTACTTTCGTAGGAAGGCGATCGATTGTTAAGGAACGATTCTCAAAGAAATATAGACATCCATCTCTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGTTTGTTTTCTTCTAATGAAATGCCTTTTTGTAGTTGAAGAAATAACTAGCGAAAAGATTGAAGCAAGGTGCATGACCAAAGCTAGGCGACTCGCGGTTTCTACTCCAGTGTTGTATGCTGTTGACCCGATATTACATACCCTGACATTTGAGTATGTGGAAGGTCGTTCTGTGAAAGATATATTACTTGAAATTGGGTCAAGTGGTGATGGTTCAAAACAGTTGAAGGACATTGCAATGCAGATCGGCGTTGCAATTGGGAAGTTGCATGATGGTGGTTTAATTCATGGTGATTTGACGACGTCTAACATGTTGGTCAGGAGTTGTACCAATGAACTTGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACTATTCCTGAAGATAAAGCAGTTGATCTATATGTGTTGGAACGAGCACTGCTATCGATGCATTCTTCATGTGGCAATGTGATGGAGCAAATTCTTGCTTCATACCGGAAGACTTCGAAACAGTGGTCGTCAACCTCGAACAAATTAGCTCAAGTGCGTCAGCGAGGCCGAAAACGCACCATGGTTGGTTAACATCTCTCTTTTTAGTGACCCTTTGTAGTAGACTCATTGAACTTCTTGAACAATGTTTATGTAATTGCAATTCTTCAGGAAAAAACCTTCAGAGTCTTGTATTTAGTTATAGTGAAGTGTCTTTGACCATAAAAGCTATATATTTAAGTGAATCAGACGATTTGTTTTTGTTGTTTTGGAGATTTATAATTAGATGTGGTGAGCTTTTTGCACTTATGAGACTATTCCTGTAAGTTCGACTTTTACAGATTCTG

Coding sequence (CDS)

ATGGAGACAAAGACGGATAGTAATGATGGCTCACTCGTTTTAATTAAGCAAGGAGCTGAAGCTAGAGTGTTTGAGTCTACTTTCGTAGGAAGGCGATCGATTGTTAAGGAACGATTCTCAAAGAAATATAGACATCCATCTCTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGTTTGTTTTCTTCTAATGAAATGCCTTTTTGTAGTTGAAGAAATAACTAGCGAAAAGATTGAAGCAAGGTGCATGACCAAAGCTAGGCGACTCGCGGTTTCTACTCCAGTGTTGTATGCTGTTGACCCGATATTACATACCCTGACATTTGAGTATGTGGAAGGTCGTTCTGTGAAAGATATATTACTTGAAATTGGGTCAAGTGGTGATGGTTCAAAACAGTTGAAGGACATTGCAATGCAGATCGGCGTTGCAATTGGGAAGTTGCATGATGGTGGTTTAATTCATGGTGATTTGACGACGTCTAACATGTTGGTCAGGAGTTGTACCAATGAACTTGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACTATTCCTGAAGATAAAGCAGTTGATCTATATGTGTTGGAACGAGCACTGCTATCGATGCATTCTTCATGTGGCAATGTGATGGAGCAAATTCTTGCTTCATACCGGAAGACTTCGAAACAGTGGTCGTCAACCTCGAACAAATTAGCTCAAGTGCGTCAGCGAGGCCGAAAACGCACCATGGTTGGTTAA

Protein sequence

METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAVCFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Homology
BLAST of Carg27498 vs. NCBI nr
Match: KAG7022296.1 (EKC/KEOPS complex subunit bud32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473.4 bits (1217), Expect = 1.2e-129
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV
Sbjct: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
           CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI
Sbjct: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 246

BLAST of Carg27498 vs. NCBI nr
Match: KAG6588447.1 (EKC/KEOPS complex subunit bud32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417.5 bits (1072), Expect = 7.8e-113
Identity = 224/246 (91.06%), Postives = 225/246 (91.46%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 28  METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 87

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPIL+TLTFEYVEGRSVKDI
Sbjct: 88  -------------------EARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDI 147

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 148 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 207

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 208 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 253

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 268 KRTMVG 253

BLAST of Carg27498 vs. NCBI nr
Match: XP_022971395.1 (EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima] >XP_022971403.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima])

HSP 1 Score: 417.5 bits (1072), Expect = 7.8e-113
Identity = 224/246 (91.06%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI
Sbjct: 61  -------------------EARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. NCBI nr
Match: XP_023529406.1 (EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 415.2 bits (1066), Expect = 3.9e-112
Identity = 223/246 (90.65%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPIL+TLTFEYVEGRSVKDI
Sbjct: 61  -------------------EARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. NCBI nr
Match: XP_022924768.1 (EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924777.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924786.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924793.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata])

HSP 1 Score: 414.5 bits (1064), Expect = 6.6e-112
Identity = 223/246 (90.65%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           METKTDS DGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   METKTDSYDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPIL+TLTFEYVEGRSVKDI
Sbjct: 61  -------------------EARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. ExPASy Swiss-Prot
Match: Q54W07 (EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-44
Identity = 105/246 (42.68%), Postives = 141/246 (57.32%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           + TK    D   +LI QGAEA+ +E+   G + IVKERFSK YRHP LD K++ KR+   
Sbjct: 27  ISTKVVKLDEIGILISQGAEAKTYETDLYGLKCIVKERFSKAYRHPILDQKISSKRILQ- 86

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              E R + K ++  +  P LY VD   + +  E+++G +VK  
Sbjct: 87  -------------------EVRSLNKCKKKGIQVPSLYLVDIGNNRIYMEFIKGETVKHY 146

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           L +   S     Q++ I  ++G  IG +H+  +IHGDLTTSNML+R  TNELV IDFGLS
Sbjct: 147 LYKNQESTQHQNQIESIMKELGNQIGIIHEMNVIHGDLTTSNMLLRESTNELVFIDFGLS 206

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           +TS   EDKAVDLYVLERA +S H +   + + IL++Y  TS        KL QVR RGR
Sbjct: 207 YTSNSVEDKAVDLYVLERAFISTHPNSEQLFQTILSNYELTSSNSKIVIQKLNQVRLRGR 252

Query: 241 KRTMVG 247
           K+T  G
Sbjct: 267 KKTCFG 252

BLAST of Carg27498 vs. ExPASy Swiss-Prot
Match: Q96S44 (EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 2.0e-42
Identity = 105/238 (44.12%), Postives = 142/238 (59.66%), Query Frame = 0

Query: 12  LVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAVCFLLMKCLFVV 71
           L L+KQGAEARVF   F GR +++K RF K YRHP+L+++L  +R              V
Sbjct: 36  LELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRR-------------TV 95

Query: 72  EEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGR-SVKDILLEIGSSGDG 131
           +       EAR + + RR  +S PV++ VD   + L  E +EG  +V+D +     +   
Sbjct: 96  Q-------EARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKT 155

Query: 132 SKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNEL--VLIDFGLSFTSTIPED 191
            + L ++A  IG  + ++HD  LIHGDLTTSNML++    +L  VLIDFGLSF S +PED
Sbjct: 156 PQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPED 215

Query: 192 KAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 247
           K VDLYVLE+A LS H +   V E  L SY  +SK+      KL +VR RGRKR+MVG
Sbjct: 216 KGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG 253

BLAST of Carg27498 vs. ExPASy Swiss-Prot
Match: Q99PW4 (EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-40
Identity = 104/238 (43.70%), Postives = 139/238 (58.40%), Query Frame = 0

Query: 12  LVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAVCFLLMKCLFVV 71
           L L++QGAEARVF   F GR ++VK RF K YRHP L+++L  +R              V
Sbjct: 27  LELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRR-------------TV 86

Query: 72  EEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGR-SVKDILLEIGSSGDG 131
           +       EAR + + RR  ++ PV++ VD   + L  E +E   +V+D +     +   
Sbjct: 87  Q-------EARALLRCRRAGIAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQSTMETEKD 146

Query: 132 SKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNEL--VLIDFGLSFTSTIPED 191
            + L D+A ++G  +  +HD  LIHGDLTTSNML+R    +L  VLIDFGLSF S +PED
Sbjct: 147 PQCLLDLARRMGQVLAGMHDQDLIHGDLTTSNMLLRRPLAQLHIVLIDFGLSFVSGLPED 206

Query: 192 KAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 247
           K VDLYVLE+A LS H       E  L SY  +SK+ S    KL +VR RGRKR+MVG
Sbjct: 207 KGVDLYVLEKAFLSTHPHTETAFEAFLKSYGASSKKSSPVLKKLDEVRLRGRKRSMVG 244

BLAST of Carg27498 vs. ExPASy Swiss-Prot
Match: Q4HYC1 (EKC/KEOPS complex subunit BUD32 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=BUD32 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.0e-34
Identity = 100/249 (40.16%), Postives = 134/249 (53.82%), Query Frame = 0

Query: 13  VLIKQGAEARVFESTFV--GRRSIVKERFSKKYRHPSLDSKLTLKRLNAVCFLLMKCLFV 72
           +LI QGAE R++++T++       +K R  K +RHP LD +LT  R+ +           
Sbjct: 34  ILITQGAEGRLYKTTYLLPDIPCALKYRPPKPWRHPILDQRLTKHRILS----------- 93

Query: 73  VEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSV-KDILLEIGSSGD 132
                    EAR + K RR  V  P +YAVD     L  E+V G  V K I   +G+  +
Sbjct: 94  ---------EARILAKCRRDGVRVPAVYAVDESAGWLMLEWVSGGPVRKSINERLGNRTE 153

Query: 133 G---SKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTN---------ELVLIDF 192
           G     +LKD+  +IG AIG +H  G++HGDLTTSNM++    N         ELV+ID 
Sbjct: 154 GIENDAELKDLMRRIGTAIGNMHKVGIVHGDLTTSNMMLEPLANPQDDNPLHGELVIIDL 213

Query: 193 GLSFTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQ 247
           GLS  S   ED+AVDLYVLERA  S H     V  ++L +Y +T KQ      KL  VR 
Sbjct: 214 GLSSGSISDEDRAVDLYVLERAFGSTHPRAECVFSEVLDAYGQTFKQAKVVLKKLEDVRM 262

BLAST of Carg27498 vs. ExPASy Swiss-Prot
Match: Q9P7N1 (EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP27G11.07c PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-33
Identity = 90/237 (37.97%), Postives = 134/237 (56.54%), Query Frame = 0

Query: 12  LVLIKQGAEARVFESTFV-GRRSIVKERFSKKYRHPSLDSKLTLKRLNAVCFLLMKCLFV 71
           L ++KQGAEA   ++ F  G   ++K R +K++RHP LD KL+ KR          CL  
Sbjct: 23  LTVVKQGAEAITIKTEFYPGEVCLLKCRPAKRWRHPILDQKLSRKR----------CL-- 82

Query: 72  VEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDG 131
                   +EAR + K   + +  P+LY +D     +  E+++G  V+D + EI    + 
Sbjct: 83  --------VEARLLAKCHYVGIKCPMLYFIDANRGQIYMEWIDGPCVRDYIREI-CECEI 142

Query: 132 SKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNEL-VLIDFGLSFTSTIPEDK 191
            K+L  +  +IG  + K+H   ++HGDLTTSNM++ S  N + + IDFGL   S   EDK
Sbjct: 143 EKKLIPLMKRIGSEVAKMHKNDIVHGDLTTSNMMLESHNNPVPIFIDFGLGSVSESEEDK 202

Query: 192 AVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 247
           AVD+YVLERAL S      ++   +L SY ++ KQ  +T  +  +VR RGRKRTM+G
Sbjct: 203 AVDIYVLERALSSTLPESESLFHHVLDSYAQSWKQSKATLRRFEEVRMRGRKRTMIG 238

BLAST of Carg27498 vs. ExPASy TrEMBL
Match: A0A6J1I5M0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111470128 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.8e-113
Identity = 224/246 (91.06%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI
Sbjct: 61  -------------------EARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. ExPASy TrEMBL
Match: A0A6J1EDH5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111432168 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.2e-112
Identity = 223/246 (90.65%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           METKTDS DGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   METKTDSYDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRLAVSTPVLYAVDPIL+TLTFEYVEGRSVKDI
Sbjct: 61  -------------------EARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. ExPASy TrEMBL
Match: A0A6J1DK25 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111021824 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.6e-108
Identity = 213/246 (86.59%), Postives = 219/246 (89.02%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRL VSTPVLYAVDP LHTLTFEYVEGR+VKDI
Sbjct: 61  -------------------EARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGSSGDG+KQL DIAMQIGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLS
Sbjct: 121 LLEIGSSGDGAKQLNDIAMQIGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. ExPASy TrEMBL
Match: A0A1S3BPU1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103492219 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.4e-105
Identity = 207/246 (84.15%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDG+L+L+KQGAEARVFESTFVGRRSIVKERFSKKYRHP LDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRL V+TPVLYAVDPIL+TLTFEYVEG SVKDI
Sbjct: 61  -------------------EARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGS+G  SK+L DIAMQIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLS
Sbjct: 121 LLEIGSNGGDSKRLSDIAMQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLS+HSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. ExPASy TrEMBL
Match: A0A5D3CF78 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003400 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.4e-105
Identity = 207/246 (84.15%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           ME KTDSNDG+L+L+KQGAEARVFESTFVGRRSIVKERFSKKYRHP LDSKLTLKRLNA 
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKARRL V+TPVLYAVDPIL+TLTFEYVEG SVKDI
Sbjct: 61  -------------------EARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
           LLEIGS+G  SK+L DIAMQIGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLS
Sbjct: 121 LLEIGSNGGDSKRLSDIAMQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
           FTSTIPEDKAVDLYVLERALLS+HSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGR
Sbjct: 181 FTSTIPEDKAVDLYVLERALLSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTMVG
Sbjct: 241 KRTMVG 226

BLAST of Carg27498 vs. TAIR 10
Match: AT5G26110.1 (Protein kinase superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 5.6e-93
Identity = 179/246 (72.76%), Postives = 200/246 (81.30%), Query Frame = 0

Query: 1   METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAV 60
           M+ + +  D SLVLIKQGAEARVFESTF GRRSIVKERFSKKYRHP LD+KLTLKRLNA 
Sbjct: 1   MDCEENVRDESLVLIKQGAEARVFESTFAGRRSIVKERFSKKYRHPILDAKLTLKRLNA- 60

Query: 61  CFLLMKCLFVVEEITSEKIEARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDI 120
                              EARCMTKAR+L V TPVLYAVD +LH+LT EY+EG SVKDI
Sbjct: 61  -------------------EARCMTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDI 120

Query: 121 LLEIGSSGDGSKQLKDIAMQIGVAIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLS 180
            LE G++G   ++L D+A QIG AI KLHDGGL HGDLTTSNMLVRS TN+LVLIDFGLS
Sbjct: 121 FLEFGTNGVVEERLDDVAAQIGAAIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLS 180

Query: 181 FTSTIPEDKAVDLYVLERALLSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGR 240
            TST+PEDKAVDLYVLERALLSMHSSCGNVM++IL +YRK+SKQWS+T NKLAQVRQRGR
Sbjct: 181 VTSTLPEDKAVDLYVLERALLSMHSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGR 226

Query: 241 KRTMVG 247
           KRTM+G
Sbjct: 241 KRTMIG 226

BLAST of Carg27498 vs. TAIR 10
Match: AT5G26110.2 (Protein kinase superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 4.0e-67
Identity = 127/163 (77.91%), Postives = 144/163 (88.34%), Query Frame = 0

Query: 84  MTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQIGV 143
           MTKAR+L V TPVLYAVD +LH+LT EY+EG SVKDI LE G++G   ++L D+A QIG 
Sbjct: 1   MTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDIFLEFGTNGVVEERLDDVAAQIGA 60

Query: 144 AIGKLHDGGLIHGDLTTSNMLVRSCTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSM 203
           AI KLHDGGL HGDLTTSNMLVRS TN+LVLIDFGLS TST+PEDKAVDLYVLERALLSM
Sbjct: 61  AIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLSVTSTLPEDKAVDLYVLERALLSM 120

Query: 204 HSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 247
           HSSCGNVM++IL +YRK+SKQWS+T NKLAQVRQRGRKRTM+G
Sbjct: 121 HSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGRKRTMIG 163

BLAST of Carg27498 vs. TAIR 10
Match: AT1G08120.1 (BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (TAIR:AT5G26110.1); Has 235 Blast hits to 235 proteins in 96 species: Archae - 21; Bacteria - 0; Metazoa - 117; Fungi - 8; Plants - 47; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-16
Identity = 44/57 (77.19%), Postives = 49/57 (85.96%), Query Frame = 0

Query: 1  METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRL 58
          M+ + +  + SLVLIKQGAEARV ESTF GRRSIVKERFSKKYRHP LD+KLTLKRL
Sbjct: 5  MDCEENVGNESLVLIKQGAEARVLESTFSGRRSIVKERFSKKYRHPILDAKLTLKRL 61

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022296.11.2e-129100.00EKC/KEOPS complex subunit bud32 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6588447.17.8e-11391.06EKC/KEOPS complex subunit bud32, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022971395.17.8e-11391.06EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima] >XP_022971403.1 EK... [more]
XP_023529406.13.9e-11290.65EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC... [more]
XP_022924768.16.6e-11290.65EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924777.1 EKC/KEOPS c... [more]
Match NameE-valueIdentityDescription
Q54W071.6e-4442.68EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE... [more]
Q96S442.0e-4244.12EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2[more]
Q99PW41.4e-4043.70EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1[more]
Q4HYC12.0e-3440.16EKC/KEOPS complex subunit BUD32 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 1... [more]
Q9P7N12.8e-3337.97EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972... [more]
Match NameE-valueIdentityDescription
A0A6J1I5M03.8e-11391.06Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EDH53.2e-11290.65Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DK251.6e-10886.59Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A1S3BPU18.4e-10584.15Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5D3CF788.4e-10584.15Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT5G26110.15.6e-9372.76Protein kinase superfamily protein [more]
AT5G26110.24.0e-6777.91Protein kinase superfamily protein [more]
AT1G08120.11.1e-1677.19BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022495Serine/threonine-protein kinase Bud32TIGRFAMTIGR03724TIGR03724coord: 78..240
e-value: 5.1E-40
score: 135.0
IPR022495Serine/threonine-protein kinase Bud32PANTHERPTHR12209O-SIALOGLYCOPROTEIN ENDOPEPTIDASEcoord: 1..64
IPR022495Serine/threonine-protein kinase Bud32PANTHERPTHR12209O-SIALOGLYCOPROTEIN ENDOPEPTIDASEcoord: 78..246
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 106..182
e-value: 1.1E-9
score: 38.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..246
score: 11.835118
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 128..243
e-value: 3.4E-35
score: 122.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 20..70
e-value: 7.9E-14
score: 52.8
NoneNo IPR availablePANTHERPTHR12209:SF1EKC/KEOPS COMPLEX SUBUNIT BUD32-LIKE ISOFORM X1coord: 1..64
NoneNo IPR availablePANTHERPTHR12209:SF1EKC/KEOPS COMPLEX SUBUNIT BUD32-LIKE ISOFORM X1coord: 78..246
IPR008266Tyrosine-protein kinase, active sitePROSITEPS00109PROTEIN_KINASE_TYRcoord: 153..165
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 14..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27498-RACarg27498-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0008033 tRNA processing
biological_process GO:0070525 tRNA threonylcarbamoyladenosine metabolic process
cellular_component GO:0000408 EKC/KEOPS complex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity