Carg27481 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg27481 vs. NCBI nr
Match: KAG7010987.1 (S-norcoclaurine synthase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.1 bits (1455), Expect = 3.5e-157
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60
           MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP
Sbjct: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120
           PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEIKEEAAANASLMLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVE 180
           EYEIKEEAAANASLMLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVE
Sbjct: 121 EYEIKEEAAANASLMLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVE 180

Query: 181 GDGGEGTVLNIIFQPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDD 240
           GDGGEGTVLNIIFQPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDD
Sbjct: 181 GDGGEGTVLNIIFQPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDD 240

Query: 241 SCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 289
           SCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV
Sbjct: 241 SCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 288

BLAST of Carg27481 vs. NCBI nr
Match: KAF4393367.1 (hypothetical protein F8388_023171 [Cannabis sativa] >KAF4396682.1 hypothetical protein G4B88_028996 [Cannabis sativa])

HSP 1 Score: 318.9 bits (816), Expect = 4.4e-83
Identity = 157/284 (55.28%), Postives = 212/284 (74.65%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQLSHE  I+APA+  W+LYG L L +L+E +L ++I+KI+VVEGDGG GT+L+L F PG
Sbjct: 4   GQLSHELEIKAPASQVWELYGTLRLVKLIEEQLKSVIEKIQVVEGDGGVGTILHLDFVPG 63

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                S+KEKFTKIDNE R+KE EVVEGGFL++GFTLYRVRL+I+E  +   +++STIEY
Sbjct: 64  AAKFKSFKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRVRLEIIEKDEGCSLIKSTIEY 123

Query: 123 EIKEEAAANASLML-GHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           E+K++ A N   M+ G LSHE  I+A A+ VW+ YG +    L+  +LPN+ +KI+++EG
Sbjct: 124 EVKDDYAENKPAMVSGQLSHEMEIKAPASQVWEFYGTIRQLNLIAKQLPNVFEKIDILEG 183

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT++++IF P      SYKEK TK+D+ENR+KE EV+EGG+LD GFTLYRVR +I+
Sbjct: 184 DGGVGTIVHLIFVPGVTRFKSYKEKLTKVDDENRVKEAEVIEGGYLDFGFTLYRVRFEII 243

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           +    S IV+STIEY++K+E A N SLVS+  L  IA  A + L
Sbjct: 244 DKDQASSIVKSTIEYEVKDESADNVSLVSLDALKAIAFIAQNEL 287

BLAST of Carg27481 vs. NCBI nr
Match: KAF4388932.1 (hypothetical protein F8388_026661 [Cannabis sativa])

HSP 1 Score: 318.2 bits (814), Expect = 7.5e-83
Identity = 158/284 (55.63%), Postives = 211/284 (74.30%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQL HE  I+APA+  W+LYG L L +L+E +L ++I+KI+VVEGDGG GT+L+L F PG
Sbjct: 4   GQLCHELEIKAPASQVWELYGTLRLVKLIEEQLKSVIEKIQVVEGDGGVGTILHLDFVPG 63

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                S+KEKFTKIDNE R+KE EVVEGGFL++GFTLYRVRL+I+E  + S +++STIEY
Sbjct: 64  AAKFKSFKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRVRLEIIEKDEGSSLIKSTIEY 123

Query: 123 EIKEEAAANASLML-GHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           EIK++   N   M+ G LSHE  I+A A+ VW+ YG +    L+  +LPN+ +KI+++EG
Sbjct: 124 EIKDDYVENKPTMVSGQLSHEMEIKAPASQVWEFYGTIRQLNLIAKQLPNVFEKIDILEG 183

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT++++IF P      SYKEK TK+D+ENR+KE EVVEGG+LD GFTLYRVR +I+
Sbjct: 184 DGGVGTIVHLIFVPGVTRFKSYKEKLTKVDDENRVKEAEVVEGGYLDFGFTLYRVRFEII 243

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           +    S IV+STIEY++K+E A N SLVS+  L  IA  A + L
Sbjct: 244 DKDQASSIVKSTIEYEVKDESADNVSLVSLDALKAIAFIAQNEL 287

BLAST of Carg27481 vs. NCBI nr
Match: KAF4388934.1 (hypothetical protein F8388_026663 [Cannabis sativa])

HSP 1 Score: 313.9 bits (803), Expect = 1.4e-81
Identity = 156/284 (54.93%), Postives = 213/284 (75.00%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQLSHE  I+APA+  W+LYG L +A+LVE +L  +I+KI++VEGDGG GT+++L F PG
Sbjct: 287 GQLSHELEIKAPASQVWELYGTLRIAKLVEEQLKTVIEKIDIVEGDGGVGTIVHLNFVPG 346

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                SYKEKFTK+DNE R+KE +V+EGG+L++GFTLYRVR +I+E  +   I++STIEY
Sbjct: 347 ATRFKSYKEKFTKVDNELRVKEADVMEGGYLELGFTLYRVRFEIIEKDEGCSIIKSTIEY 406

Query: 123 EIKEE-AAANASLMLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           E+K++ A  N +++ G L HE   +A A+ VW+L G L L +L+E +L ++I+KI+VVEG
Sbjct: 407 ELKDDYAENNTNMVSGQLCHELEFKAPASQVWELCGTLRLVKLIEEQLKSVIEKIDVVEG 466

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT L++ F P      S KEKFTKIDNE R+KE EVVEGGFL++GFTLYR+RL+I+
Sbjct: 467 DGGVGTTLHLDFVPGVAKFKSLKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRIRLEII 526

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           E  +D  I++STIEY+IK++ A N SLVS+  L  IA    +HL
Sbjct: 527 EKDEDCSIIKSTIEYEIKDDYAENVSLVSLDALAAIALIVQNHL 570

BLAST of Carg27481 vs. NCBI nr
Match: KZM92218.1 (hypothetical protein DCAR_020417 [Daucus carota subsp. sativus])

HSP 1 Score: 302.0 bits (772), Expect = 5.6e-78
Identity = 154/309 (49.84%), Postives = 209/309 (67.64%), Query Frame = 0

Query: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60
           MLG +S E  + APA+  W++YG LE+A +VE  L+ L+QKIEV+EGDG  GT+LN++F 
Sbjct: 1   MLGTVSDEIEVNAPASALWEVYGTLEIAAVVEKGLAALVQKIEVLEGDGSAGTLLNIVFQ 60

Query: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120
           PG    PSYKEK+TK+DNE R+KE EVVEGG+L+IGF  Y VR +++E  +++ I  +TI
Sbjct: 61  PGGFAFPSYKEKYTKVDNEKRVKEAEVVEGGYLEIGFNKYLVRFEVIEKDEENSITRATI 120

Query: 121 EYEIKEEAAANASL-----------------------MLGHLSHEAAIQASATVVWQLYG 180
           EY+IKEE   NAS                        M G +S E  + A A+VVW++Y 
Sbjct: 121 EYDIKEELVDNASFVSIDPLVGIMNLVANHVLVKKGKMFGTISGEVEVNAPASVVWEVYS 180

Query: 181 GLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQPS------YKEKFTKIDNENRIK 240
            L+LA +VE  L ++++KIEVVEGDG  GTVL ++F+P       YKEKF  ID+E R+K
Sbjct: 181 SLQLAAIVEKGLTDVVEKIEVVEGDGSVGTVLKLLFRPGVAAFPYYKEKFMMIDHEKRVK 240

Query: 241 ETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDI 281
           +  VVEGG+LD+GF  Y +RL+I++  + SCI +STIEYDIKE+ AANASLVSI PL+ +
Sbjct: 241 DVMVVEGGYLDVGFERYLIRLEIIDKDEKSCITKSTIEYDIKEDYAANASLVSIDPLMVV 300

BLAST of Carg27481 vs. ExPASy Swiss-Prot
Match: A0A3G5BB24 (Norbelladine synthase OS=Narcissus pseudonarcissus OX=39639 GN=NBS PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.3e-32
Identity = 71/160 (44.38%), Postives = 103/160 (64.38%), Query Frame = 0

Query: 135 MLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIF- 194
           M G LSHE  +   A  +WQ+Y  L LA+L    LP +I K+EV EGDGG GT+L + + 
Sbjct: 1   MKGSLSHELEVSLPADQLWQVYSTLRLAQLSAELLPTVISKVEVEEGDGGVGTLLRVTYA 60

Query: 195 -----QPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 254
                   +KE+F KID+E R+KE   VEGG LD+GF+ Y +RL+I+E G +S +++ST+
Sbjct: 61  LGIPGMKYHKERFVKIDHEKRLKEALFVEGGHLDLGFSSYLIRLEILEKGHNSSVIKSTV 120

Query: 255 EYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 289
           EY++ EE AANAS  +  P + I  A ++HLL  K + ++
Sbjct: 121 EYEVDEEHAANASFATTDPFMIIGGAVSEHLLQKKSNCSI 160

BLAST of Carg27481 vs. ExPASy Swiss-Prot
Match: Q4QTJ2 (S-norcoclaurine synthase 1 OS=Papaver somniferum OX=3469 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.1e-23
Identity = 55/135 (40.74%), Postives = 89/135 (65.93%), Query Frame = 0

Query: 4   QLSHEAVIQAPATVAWQLYGGLELARLVEN-RLSNLIQKIEVVEGDGGEGTVLNLIFPPG 63
           ++++E  +Q  A   W +Y   ++ RL+ +  L  + +K++V+ G+GG GTVL++ FP  
Sbjct: 42  EITYETEVQTSADSIWNVYSSPDIPRLLRDVLLPGVFEKLDVIAGNGGVGTVLDIAFP-- 101

Query: 64  LGGAP-SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIE 123
           LG  P  YKEKF KI++E R+KE  ++EGG+LD+G T Y  R+ I E   +SC++ES+I 
Sbjct: 102 LGAVPRRYKEKFVKINHEKRLKEVVMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSII 161

Query: 124 YEIKEEAAANASLML 137
           YE+KEE A   + ++
Sbjct: 162 YEVKEEYAGKMAKLI 174

BLAST of Carg27481 vs. ExPASy Swiss-Prot
Match: Q4QTJ1 (S-norcoclaurine synthase 2 OS=Papaver somniferum OX=3469 GN=NCS2 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 54/134 (40.30%), Postives = 88/134 (65.67%), Query Frame = 0

Query: 4   QLSHEAVIQAPATVAWQLYGGLELARLVEN-RLSNLIQKIEVVEGDGGEGTVLNLIFPPG 63
           ++ ++  +   A   W +Y   ++ RL+ +  L  + QK++V+EG+GG GTVL+++FPPG
Sbjct: 42  EIRYDLEVPTSADSIWSVYSCPDIPRLLRDVLLPGVFQKLDVIEGNGGVGTVLDIVFPPG 101

Query: 64  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 123
                SYKEKF  I++E R+KE  ++EGG+LD+G T Y  R+ I E   +SC++ES+I Y
Sbjct: 102 -AVPRSYKEKFVNINHEKRLKEVIMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSIIY 161

Query: 124 EIKEEAAANASLML 137
           E+KEE A   + ++
Sbjct: 162 EVKEEYAGKMAKLI 174

BLAST of Carg27481 vs. ExPASy Swiss-Prot
Match: Q67A25 (S-norcoclaurine synthase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.8e-21
Identity = 49/123 (39.84%), Postives = 78/123 (63.41%), Query Frame = 0

Query: 5   LSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPGLG 64
           + HE  + A A   W +Y    LA+ + + L    +K+E++ GDGG GT+L++ F PG  
Sbjct: 46  IHHELEVAASADDIWTVYSWPGLAKHLPDLLPGAFEKLEII-GDGGVGTILDMTFVPG-E 105

Query: 65  GAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEYEI 124
               YKEKF  +DNE+R+K+ +++EGG+LD+G T Y   + +V  G DSC+++S+ EY +
Sbjct: 106 FPHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHV 165

Query: 125 KEE 128
           K E
Sbjct: 166 KPE 166

BLAST of Carg27481 vs. ExPASy Swiss-Prot
Match: A2A1A1 (S-norcoclaurine synthase 2 OS=Coptis japonica OX=3442 GN=PR10A PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.8e-18
Identity = 54/149 (36.24%), Postives = 82/149 (55.03%), Query Frame = 0

Query: 5   LSHEAVIQAPATVAWQLYGGLELARLVENRL-SNLIQKIEVVEGDGGEGTVLNLIFPPGL 64
           L HE  + A A   W + G  EL   + + L + +  K E+  GDGGEG++L++ FPPG 
Sbjct: 41  LYHELEVAASADEVWSVEGSPELGLHLPDLLPAGIFAKFEIT-GDGGEGSILDMTFPPG- 100

Query: 65  GGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEYE 124
                Y+EKF   D++NR K  E ++G F D+G T Y   +++V  G DSC+++ST EY 
Sbjct: 101 QFPHHYREKFVFFDHKNRYKLVEQIDGDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYH 160

Query: 125 IKEEAAANASLMLGHLSHEAAIQASATVV 153
           +K E A     ++  +      +A A VV
Sbjct: 161 VKPEFAKIVKPLIDTVPLAIMSEAIAKVV 187

BLAST of Carg27481 vs. ExPASy TrEMBL
Match: A0A7J6HDD3 (Bet_v_1 domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_023171 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 2.1e-83
Identity = 157/284 (55.28%), Postives = 212/284 (74.65%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQLSHE  I+APA+  W+LYG L L +L+E +L ++I+KI+VVEGDGG GT+L+L F PG
Sbjct: 4   GQLSHELEIKAPASQVWELYGTLRLVKLIEEQLKSVIEKIQVVEGDGGVGTILHLDFVPG 63

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                S+KEKFTKIDNE R+KE EVVEGGFL++GFTLYRVRL+I+E  +   +++STIEY
Sbjct: 64  AAKFKSFKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRVRLEIIEKDEGCSLIKSTIEY 123

Query: 123 EIKEEAAANASLML-GHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           E+K++ A N   M+ G LSHE  I+A A+ VW+ YG +    L+  +LPN+ +KI+++EG
Sbjct: 124 EVKDDYAENKPAMVSGQLSHEMEIKAPASQVWEFYGTIRQLNLIAKQLPNVFEKIDILEG 183

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT++++IF P      SYKEK TK+D+ENR+KE EV+EGG+LD GFTLYRVR +I+
Sbjct: 184 DGGVGTIVHLIFVPGVTRFKSYKEKLTKVDDENRVKEAEVIEGGYLDFGFTLYRVRFEII 243

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           +    S IV+STIEY++K+E A N SLVS+  L  IA  A + L
Sbjct: 244 DKDQASSIVKSTIEYEVKDESADNVSLVSLDALKAIAFIAQNEL 287

BLAST of Carg27481 vs. ExPASy TrEMBL
Match: A0A7J6H145 (Bet_v_1 domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_026661 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.6e-83
Identity = 158/284 (55.63%), Postives = 211/284 (74.30%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQL HE  I+APA+  W+LYG L L +L+E +L ++I+KI+VVEGDGG GT+L+L F PG
Sbjct: 4   GQLCHELEIKAPASQVWELYGTLRLVKLIEEQLKSVIEKIQVVEGDGGVGTILHLDFVPG 63

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                S+KEKFTKIDNE R+KE EVVEGGFL++GFTLYRVRL+I+E  + S +++STIEY
Sbjct: 64  AAKFKSFKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRVRLEIIEKDEGSSLIKSTIEY 123

Query: 123 EIKEEAAANASLML-GHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           EIK++   N   M+ G LSHE  I+A A+ VW+ YG +    L+  +LPN+ +KI+++EG
Sbjct: 124 EIKDDYVENKPTMVSGQLSHEMEIKAPASQVWEFYGTIRQLNLIAKQLPNVFEKIDILEG 183

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT++++IF P      SYKEK TK+D+ENR+KE EVVEGG+LD GFTLYRVR +I+
Sbjct: 184 DGGVGTIVHLIFVPGVTRFKSYKEKLTKVDDENRVKEAEVVEGGYLDFGFTLYRVRFEII 243

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           +    S IV+STIEY++K+E A N SLVS+  L  IA  A + L
Sbjct: 244 DKDQASSIVKSTIEYEVKDESADNVSLVSLDALKAIAFIAQNEL 287

BLAST of Carg27481 vs. ExPASy TrEMBL
Match: A0A7J6H159 (Bet_v_1 domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_026663 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 6.8e-82
Identity = 156/284 (54.93%), Postives = 213/284 (75.00%), Query Frame = 0

Query: 3   GQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFPPG 62
           GQLSHE  I+APA+  W+LYG L +A+LVE +L  +I+KI++VEGDGG GT+++L F PG
Sbjct: 287 GQLSHELEIKAPASQVWELYGTLRIAKLVEEQLKTVIEKIDIVEGDGGVGTIVHLNFVPG 346

Query: 63  LGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEY 122
                SYKEKFTK+DNE R+KE +V+EGG+L++GFTLYRVR +I+E  +   I++STIEY
Sbjct: 347 ATRFKSYKEKFTKVDNELRVKEADVMEGGYLELGFTLYRVRFEIIEKDEGCSIIKSTIEY 406

Query: 123 EIKEE-AAANASLMLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEG 182
           E+K++ A  N +++ G L HE   +A A+ VW+L G L L +L+E +L ++I+KI+VVEG
Sbjct: 407 ELKDDYAENNTNMVSGQLCHELEFKAPASQVWELCGTLRLVKLIEEQLKSVIEKIDVVEG 466

Query: 183 DGGEGTVLNIIFQP------SYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIV 242
           DGG GT L++ F P      S KEKFTKIDNE R+KE EVVEGGFL++GFTLYR+RL+I+
Sbjct: 467 DGGVGTTLHLDFVPGVAKFKSLKEKFTKIDNEQRVKEVEVVEGGFLELGFTLYRIRLEII 526

Query: 243 ENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDIAQAANDHL 280
           E  +D  I++STIEY+IK++ A N SLVS+  L  IA    +HL
Sbjct: 527 EKDEDCSIIKSTIEYEIKDDYAENVSLVSLDALAAIALIVQNHL 570

BLAST of Carg27481 vs. ExPASy TrEMBL
Match: A0A164WTB2 (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_020417 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 2.7e-78
Identity = 154/309 (49.84%), Postives = 209/309 (67.64%), Query Frame = 0

Query: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60
           MLG +S E  + APA+  W++YG LE+A +VE  L+ L+QKIEV+EGDG  GT+LN++F 
Sbjct: 1   MLGTVSDEIEVNAPASALWEVYGTLEIAAVVEKGLAALVQKIEVLEGDGSAGTLLNIVFQ 60

Query: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120
           PG    PSYKEK+TK+DNE R+KE EVVEGG+L+IGF  Y VR +++E  +++ I  +TI
Sbjct: 61  PGGFAFPSYKEKYTKVDNEKRVKEAEVVEGGYLEIGFNKYLVRFEVIEKDEENSITRATI 120

Query: 121 EYEIKEEAAANASL-----------------------MLGHLSHEAAIQASATVVWQLYG 180
           EY+IKEE   NAS                        M G +S E  + A A+VVW++Y 
Sbjct: 121 EYDIKEELVDNASFVSIDPLVGIMNLVANHVLVKKGKMFGTISGEVEVNAPASVVWEVYS 180

Query: 181 GLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQPS------YKEKFTKIDNENRIK 240
            L+LA +VE  L ++++KIEVVEGDG  GTVL ++F+P       YKEKF  ID+E R+K
Sbjct: 181 SLQLAAIVEKGLTDVVEKIEVVEGDGSVGTVLKLLFRPGVAAFPYYKEKFMMIDHEKRVK 240

Query: 241 ETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTIEYDIKEEDAANASLVSIQPLIDI 281
           +  VVEGG+LD+GF  Y +RL+I++  + SCI +STIEYDIKE+ AANASLVSI PL+ +
Sbjct: 241 DVMVVEGGYLDVGFERYLIRLEIIDKDEKSCITKSTIEYDIKEDYAANASLVSIDPLMVV 300

BLAST of Carg27481 vs. ExPASy TrEMBL
Match: A0A6J1FUB4 (S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448205 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 5.6e-76
Identity = 153/160 (95.62%), Postives = 153/160 (95.62%), Query Frame = 0

Query: 135 MLGHLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQ 194
           MLG LSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQ
Sbjct: 1   MLGQLSHEAAIQASATVVWQLYGGLELARLVENRLPNLIKKIEVVEGDGGEGTVLNIIFQ 60

Query: 195 PS------YKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 254
           PS      YKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI
Sbjct: 61  PSLGGAPGYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 255 EYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 289
           EYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV
Sbjct: 121 EYDIKEEDAANASLVSIQPLIDIAQAANDHLLHNKQHKNV 160