Carg27474 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27474
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Aminoacyl-tRNA hydrolase
Location	Carg_Chr20: 4815086 .. 4815470 (+)
RNA-Seq Expression	Carg27474
Synteny	Carg27474

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTGACATGGCTGAGTGCCATTGTAGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTCTGTCTCTCTGTTTCTCTCTTGCTGTCTATTGGAAAATCATTCTCGACGACTTTAAGACGTTCTCTCTTTTACTCTCTCTAAAAAAATATAACAACATTTCACTGTGTCTCTTCAGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAAAATTGCTGCTCAATGCA

mRNA sequence

ATGGATCTGACATGGCTGAGTGCCATTGTAGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAAAATTGCTGCTCAATGCA

Coding sequence (CDS)

ATGGATCTGACATGGCTGAGTGCCATTGTAGTTGGGGCAAGCTGCCTTGTTTTGGGATACTGTATTGGTTCGAAGTATCCTCCTCGCATCTTTATCAAAGCTAAATTAGCCAAAATCAACCAATCCAGCAGAAATGGGAAGAAGGATAAGCCTAAAGAGCCATTGGAGGTCGAAAATTTGGCTGACATACTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAAAATTGCTGCTCAATGCA

Protein sequence

MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENLADILDDFKMVLVVRNDLKMGKGKKLLLNA

Homology

[Close]

BLAST of Carg27474 vs. NCBI nr
Match: KAG7010994.1 (hypothetical protein SDJN02_27792, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 176.8 bits (447), Expect = 8.3e-41
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGKKLLLNA 90
          ADILDDFKMVLVVRNDLKMGKGKKLLLNA
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGKKLLLNA 89

[Close]

BLAST of Carg27474 vs. NCBI nr
Match: KAG7010995.1 (Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 166.8 bits (421), Expect = 8.6e-38
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 83

[Close]

BLAST of Carg27474 vs. NCBI nr
Match: XP_023511828.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 165.6 bits (418), Expect = 1.9e-37
Identity = 82/83 (98.80%), Postives = 83/83 (100.00%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKA+LAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKARLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 83

[Close]

BLAST of Carg27474 vs. NCBI nr
Match: XP_022944765.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 165.2 bits (417), Expect = 2.5e-37
Identity = 82/83 (98.80%), Postives = 83/83 (100.00%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLK+GKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKIGKGK 83

[Close]

BLAST of Carg27474 vs. NCBI nr
Match: XP_022986617.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 6.2e-36
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLS IVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLS-IVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 82

[Close]

BLAST of Carg27474 vs. ExPASy TrEMBL
Match: A0A6J1FZ04 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-37
Identity = 82/83 (98.80%), Postives = 83/83 (100.00%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLK+GKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKIGKGK 83

[Close]

BLAST of Carg27474 vs. ExPASy TrEMBL
Match: A0A6J1J827 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.0e-36
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLS IVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL
Sbjct: 1  MDLTWLS-IVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 82

[Close]

BLAST of Carg27474 vs. ExPASy TrEMBL
Match: A0A6J1G686 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.3e-34
Identity = 77/83 (92.77%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGA CLVLGYCIGSKYPPRIFIKAKLAK N+SSR GKK KPKEPLEVENL
Sbjct: 1  MDLTWLSAIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRTGKKGKPKEPLEVENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADIL+DFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILEDFKMVLVVRNDLKMGKGK 83

[Close]

BLAST of Carg27474 vs. ExPASy TrEMBL
Match: A0A6J1L379 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111499994 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-33
Identity = 75/83 (90.36%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGA CLVLGYCI SKYPPRIFIKAKLAK N+SSR+GKK KPKEPLE+ENL
Sbjct: 1  MDLTWLSAIVVGAGCLVLGYCIRSKYPPRIFIKAKLAKSNESSRSGKKGKPKEPLEIENL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADIL+DFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILEDFKMVLVVRNDLKMGKGK 83

[Close]

BLAST of Carg27474 vs. ExPASy TrEMBL
Match: A0A6J1D5N0 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-32
Identity = 74/83 (89.16%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSAIVVGA CLVLGY IGSKYPPRIF KAKLAK N+SSR+GKKDKPKE LE+E+L
Sbjct: 1  MDLTWLSAIVVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 83

[Close]

BLAST of Carg27474 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-17
Identity = 49/83 (59.04%), Postives = 60/83 (72.29%), Query Frame = 0


Query: 1  MDLTWLSAIVVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVENL 60
          MDLTWLSA++VGA+   LGYCIG++       K  +A ++   ++ K    K PLE+E L
Sbjct: 1  MDLTWLSALIVGAA---LGYCIGTRRS-----KPVVAAVD---KDIKTQSSKSPLEIEKL 60

Query: 61 ADILDDFKMVLVVRNDLKMGKGK 84
          ADILDDFKMVLVVRNDLKMGKGK
Sbjct: 61 ADILDDFKMVLVVRNDLKMGKGK 72

[Close]

BLAST of Carg27474 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 81.6 bits (200), Expect = 3.4e-16
Identity = 48/85 (56.47%), Postives = 58/85 (68.24%), Query Frame = 0


Query: 1  MDLTW-LSAIVVGASCLVLGYCIGS-KYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVE 60
          MDL W LS ++VGA+   LGY I + +   RIF+ +K   I+  S   KK K KEPLE+E
Sbjct: 1  MDLVWLLSVLLVGAA---LGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIE 60

Query: 61 NLADILDDFKMVLVVRNDLKMGKGK 84
           LAD   +FKMVLVVRNDLKMGKGK
Sbjct: 61 KLADFRKNFKMVLVVRNDLKMGKGK 82

[Close]

BLAST of Carg27474 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 81.6 bits (200), Expect = 3.4e-16
Identity = 48/85 (56.47%), Postives = 58/85 (68.24%), Query Frame = 0


Query: 1  MDLTW-LSAIVVGASCLVLGYCIGS-KYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVE 60
          MDL W LS ++VGA+   LGY I + +   RIF+ +K   I+  S   KK K KEPLE+E
Sbjct: 1  MDLVWLLSVLLVGAA---LGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIE 60

Query: 61 NLADILDDFKMVLVVRNDLKMGKGK 84
           LAD   +FKMVLVVRNDLKMGKGK
Sbjct: 61 KLADFRKNFKMVLVVRNDLKMGKGK 82

The following BLAST results are available for this feature:

BLAST of Carg27474 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010994.1	8.3e-41	100.00	hypothetical protein SDJN02_27792, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG7010995.1	8.6e-38	100.00	Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_023511828.1	1.9e-37	98.80	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. ...	[more]
XP_022944765.1	2.5e-37	98.80	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita moschata]	[more]
XP_022986617.1	6.2e-36	98.80	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg27474 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg27474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ04	1.2e-37	98.80	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1	[more]
A0A6J1J827	3.0e-36	98.80	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1	[more]
A0A6J1G686	1.3e-34	92.77	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1	[more]
A0A6J1L379	1.1e-33	90.36	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111499994 PE=4 SV=1	[more]
A0A6J1D5N0	1.2e-32	89.16	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=...	[more]

BLAST of Carg27474 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16870.1	1.8e-17	59.04	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.1	3.4e-16	56.47	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.2	3.4e-16	56.47	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 1..83
None	No IPR available	PANTHER	PTHR12649:SF23	SUBFAMILY NOT NAMED	coord: 1..83
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 65..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27474-RA	Carg27474-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity