Carg27436 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27436
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr04: 435930 .. 437258 (+)
RNA-Seq ExpressionCarg27436
SyntenyCarg27436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

mRNA sequence

ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

Coding sequence (CDS)

ATGATTTCCTCTTGGAGACGACGGAGGGCCACCCGGCGAACGGCAAGGGGCACCGGCAGTGACATGAGAGAGCTGACGATCCCGAGCCATTTCCGGTGTCCAATTTCGCTGGACATGATGAAAGATCCAGTGACTTTGTCTACTGGAATCACATATGATCGTGCTAGTATTGAGACATGGATTGAAGGAGGGAATTTCAGCTGCCCCATCACCAACCAGCCATTACAAACCCTTGATTCCATTCCAAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAAAATAAAGCTTACGGGGTCGAGAGAATTCCGACGCCGAGGGTTCCGGTGAGTCCCGTGCAGATTCGGGACATTCTCGAGAAGATTACCGCTGCAGCACGGCGGGGGGATGGTGACGGATGCAAGTCGTCGGTCGAGAAGATCAAGATGTTGGCAAAAGAAAGTGAAAGGAACAAGAAATGTATTATGGGCAATGGAGCAAGCCATGTGATATCATCAGCATTCCAAGCGTTTTCAAAACAAGAACCATTGGAGAAAAGCATAGAGATTTTAGAAGAAATGATATCAGCCATGGCAATCATGTTCCCTCTCCAAGAAGAATCCATTAAAAACCTCAAATCAAAAGCTTCATTACAAGCCCTCGTGTTGTTCATGAAAGGAGGAGTTTTATCAAGAAGAAGAAATTCAATTTTGATATTGAAACAAATCATTTCATCATACCCACAAAAAGCAACAAAATTAGGTGAAATCCAAGGAGCATTAGAGGGATTAGTGAAGCTCATAAAAGAGCCCATTTGTTCATCATCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCTTCATCTTCCAATCCACAAAGATTCACAAAAGCCCTTTTAGAAATGGGGTTCGTCTCTTTGCTACTCGAAACGCTCGTCGACGCCGAGAGAAGCGTTTGCGAGAGGGCGTTGGGAGCGTTCGATGGGATTTGCGAGAGCAAAGAAGGAAGAGAAGAGGCGTACGCGCACGCGTTGACGATGCCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACGGAGTTATCGGTGTCGATTGTTTGGAAGCTTGTAAAGCATGAGAGTGAAGAAGAAGAAGAAGATGGAAGGGTTAAAGTAGAAGCACTTCAAGTGGGAGCGTTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTATTGAATAATCTTCATAGGGATAGCTTAGAATGTATTGATTCATTGGACTTTAAGGGTATCAAAAGACCCTTTTGA

Protein sequence

MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDFKGIKRPF
Homology
BLAST of Carg27436 vs. NCBI nr
Match: KAG7030606.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.1 bits (2208), Expect = 2.6e-244
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRDSLECIDSLDFKGIKRPF
Sbjct: 421 NLHRDSLECIDSLDFKGIKRPF 442

BLAST of Carg27436 vs. NCBI nr
Match: KAG6599923.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850.1 bits (2195), Expect = 8.4e-243
Identity = 440/442 (99.55%), Postives = 440/442 (99.55%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGG LSRRRNSILILKQIIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGDLSRRRNSILILKQIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRDSLECIDSLDFKGIKRPF
Sbjct: 421 NLHRDSLECIDSLDFKGIKRPF 442

BLAST of Carg27436 vs. NCBI nr
Match: XP_023512745.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842.0 bits (2174), Expect = 2.3e-240
Identity = 436/442 (98.64%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENK YGVERIPTPRVPVSPVQI DI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKDYGVERIPTPRVPVSPVQIGDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGG LSRRRNSILILK+IIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGDLSRRRNSILILKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRD LECIDSLDFKGIKRPF
Sbjct: 421 NLHRDRLECIDSLDFKGIKRPF 442

BLAST of Carg27436 vs. NCBI nr
Match: XP_022942705.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 833.6 bits (2152), Expect = 8.2e-238
Identity = 433/442 (97.96%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWR+RRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRQRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKS+ASLQALVLFMKGG LSRRRNSILILKQIIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSEASLQALVLFMKGGDLSRRRNSILILKQIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTK LLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKVLLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICE KEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICERKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGR KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRAKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRDSLECIDSLDFKGIKRPF
Sbjct: 421 NLHRDSLECIDSLDFKGIKRPF 440

BLAST of Carg27436 vs. NCBI nr
Match: XP_022995728.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 817.8 bits (2111), Expect = 4.6e-233
Identity = 423/442 (95.70%), Postives = 429/442 (97.06%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTG  MRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGGCMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVP SPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           L+KITAA RRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMIS MAIMFPLQEESIKNLKS+ASLQAL LFM+GG LSRRRNSILILK+IIS
Sbjct: 181 KSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL+MG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLQMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERS+CERALG FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRD LECIDSLDFK IKRPF
Sbjct: 421 NLHRDRLECIDSLDFKDIKRPF 440

BLAST of Carg27436 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.6e-98
Identity = 198/420 (47.14%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPN 83
           E+TIP  F+CPIS+D+MKDPV +STGITYDR SIETWI  GN +CP+TN  L T D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 84  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKI 143
           H IRKMIQ WCVE  +  ++RIPTPRVP+ P ++ +I  K+++A RRGD + C   +EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 144 KMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEES 203
           K L  ESE+N+KC+  N    V+   F  FS     EK   +L E++S +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 204 IKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLI 263
           I  L S  S + +   +K    S R+N+  I+K+I+S    +        G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 264 KEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGA 323
           ++ + SSS K+SL   Y ++             LE+G VS+ +E +VDAE SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQK---PEIASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 324 FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRV 383
            D ICE++ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    ED   
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED--- 387

Query: 384 KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSL-DFKGIKRPF 443
              A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK IK+PF
Sbjct: 388 ---AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Carg27436 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.9e-84
Identity = 177/427 (41.45%), Postives = 262/427 (61.36%), Query Frame = 0

Query: 20  SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLD 79
           S+  E+TIPS F+CPIS ++MKDPV +++GITYDR +IE W E G  +CP+TN  L +L+
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 80  SIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSS 139
            IPNH IR+MIQ WC  +   G+ERIPTPRVPV+  Q+ +I E+++AA RRGD   C   
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 140 VEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL 199
           V K+  L KESERN+KC+  NGA  V+   F AFS+      +  +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSEN---ANASLLLEETVSVLTWMLPI 205

Query: 200 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGL 259
             E    L + +S   LV  ++ G     +N+  ++K+++         L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNG----DQNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 260 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCER 319
           +K I      S+   SL   +H+I ++   Q      LE+  V++ +E LVD+E SVCE+
Sbjct: 266 MKSINR---DSTCVNSLISIHHMILTN---QETVSRFLELDLVNITVEMLVDSENSVCEK 325

Query: 320 ALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEE 379
           AL   + ICE+KEGRE+   + L +P++VKKIL++S+   +  VS++WK+ K        
Sbjct: 326 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK------SG 385

Query: 380 DGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS----LECIDSLDF 439
           DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + + +         S++F
Sbjct: 386 DGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEF 431

Query: 440 KGIKRPF 443
           K +K+PF
Sbjct: 446 KHVKKPF 431

BLAST of Carg27436 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.0e-54
Identity = 144/419 (34.37%), Postives = 223/419 (53.22%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNH 84
           + IP HFRCPISLD+M DPVT+STG TYDR SI++WI  GN +CP+T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 85  NIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SVE 144
            +R++IQ+WCV N++ GVERIPTP+ P  P+ +R +L +  A+A  G     +S   ++ 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQ--ASAITGTHVSVRSRAAAIR 131

Query: 145 KIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAI--MFPL 204
           +++ LA++SE+N+  I G+ A  ++      F+  E    S E++ E ++ + +  M   
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTET 191

Query: 205 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKL--GEIQGALE 264
           + E++ +  S+      +LF     +  R N+  +++ +++       KL         E
Sbjct: 192 ECEAVASDPSRVGFMTRLLF--DSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 265 GLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAERSV 324
           G++ L+K PI   S + +L I    I +    ++     +  G   +L++ L  D +R  
Sbjct: 252 GVLDLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 311

Query: 325 CERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEE 384
            ER L   + +C   EG      HALT+P++VK ILRVSD ATE +   +  L   E   
Sbjct: 312 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEE-- 371

Query: 385 EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDF 436
                R + EA   G   +LLLL+Q  C+E  K KA  LLKLL +   D    + S DF
Sbjct: 372 -----RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD-STVHSDDF 413

BLAST of Carg27436 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.3e-52
Identity = 142/421 (33.73%), Postives = 230/421 (54.63%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIE-GGNFSCPITNQPLQTLDSIPN 84
           + IP HFRCPISL++M+DPVT+ TG TYDRASIE+W+  G N +CP+T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 85  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SV 144
           H +R++IQ+WCV N++ GVERIPTP+ P  P  +R +L +  A+A  G     +S   ++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQ--ASAITGTHVSVRSRAAAL 131

Query: 145 EKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQ 204
            +++  A++S++N+  I  + A+ ++      FS+      S E++ E + A+ +M P+ 
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESL-ALLVMLPIT 191

Query: 205 E--ESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEI---QGA 264
           E  + +        ++ L   +    +  R N+  ++ +I+S+  + A   G I   +  
Sbjct: 192 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESV 251

Query: 265 LEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAER 324
            EG++ L++ PI   S + +L I    + +  + +      +  G   +L++ L  D +R
Sbjct: 252 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 311

Query: 325 SVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHES 384
              ERAL   + +C + EG      HALT+P++VK ILRVSD ATE +   +  L   E 
Sbjct: 312 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEE 371

Query: 385 EEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLD 436
                  R + EA   G   +LLL++Q  C+E  K+KA +LLKLL +   D     +S D
Sbjct: 372 -------RWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDD 412

BLAST of Carg27436 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.6e-50
Identity = 134/411 (32.60%), Postives = 222/411 (54.01%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWI-EGGNFSCPITNQPLQTLDSIP 83
           E+ IP  F CPISL++MKDPV +STGITYDR SIE W+  G   SCP+T Q +   D  P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 84  NHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEK 143
           NH +R++IQ WC  N +YGVERIPTPR P+   +I  ++    +A+   +   C   +++
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC---LKR 128

Query: 144 IKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPL-EKSIEILEEMISAMAIMFPLQE 203
           ++ +  E+  NK+C+   G    +++     S+   L ++++ +L  + ++  ++    +
Sbjct: 129 LRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVL----K 188

Query: 204 ESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQI--ISSYPQKATKLGEIQGALEGL 263
             + N K    +++L   M+ G+   R  + L+LK I  ++   Q  T   E+      +
Sbjct: 189 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV---FTEV 248

Query: 264 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVD----AERS 323
           V+++ + I   + KA++ I  +I     N  +     +E G +S+++E L+D    +ER 
Sbjct: 249 VQILDDRISQKATKAAMHILVNICPWGRNRHK----AVEAGVISVIIELLMDESFTSERR 308

Query: 324 VCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESE 383
             E A+   D +C+  EGR E   H   + V+ KKILRVS  A++ +V ++  + +  + 
Sbjct: 309 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 368

Query: 384 EEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS 427
                  +  E LQ+G   KL L+LQV C   TKEKA ELLKL   + +DS
Sbjct: 369 P-----ALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDS 398

BLAST of Carg27436 vs. ExPASy TrEMBL
Match: A0A6J1FWS8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447660 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.9e-238
Identity = 433/442 (97.96%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWR+RRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRQRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMISAMAIMFPLQEESIKNLKS+ASLQALVLFMKGG LSRRRNSILILKQIIS
Sbjct: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSEASLQALVLFMKGGDLSRRRNSILILKQIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTK LLEMG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKVLLEMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERSVCERALGAFDGICE KEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSVCERALGAFDGICERKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGR KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRAKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRDSLECIDSLDFKGIKRPF
Sbjct: 421 NLHRDSLECIDSLDFKGIKRPF 440

BLAST of Carg27436 vs. ExPASy TrEMBL
Match: A0A6J1K8U9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.2e-233
Identity = 423/442 (95.70%), Postives = 429/442 (97.06%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRRTARGTG  MRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRTARGTGGCMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVP SPVQIRDI
Sbjct: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQIRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           L+KITAA RRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQA SKQEPLE
Sbjct: 121 LDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPLE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           KSIEILEEMIS MAIMFPLQEESIKNLKS+ASLQAL LFM+GG LSRRRNSILILK+IIS
Sbjct: 181 KSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMG 300
           SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL+MG
Sbjct: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLQMG 300

Query: 301 FVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360
           FVSLLLETLVDAERS+CERALG FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE
Sbjct: 301 FVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATE 360

Query: 361 LSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420
           LSVSIVWKLVKHES  EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN
Sbjct: 361 LSVSIVWKLVKHES--EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLN 420

Query: 421 NLHRDSLECIDSLDFKGIKRPF 443
           NLHRD LECIDSLDFK IKRPF
Sbjct: 421 NLHRDRLECIDSLDFKDIKRPF 440

BLAST of Carg27436 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.0e-202
Identity = 364/445 (81.80%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRATRR ARG  S   ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATRRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+R+I
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+ AAARRGD DGCKS VEKIK L KESERNKKCI+ NG  + +SS F+AFS  E  E
Sbjct: 121 LEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM + FPLQEESIK+LKS+ SLQ+LV F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LGEIQGALEGL+KLIK+PICSSS+KASLFITYH+IA   SSSN +RFTK LL
Sbjct: 241 SYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSV IVWKLVKHES +EEEDG +KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVCIVWKLVKHES-KEEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of Carg27436 vs. ExPASy TrEMBL
Match: A0A1S3CPH4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.8e-202
Identity = 362/445 (81.35%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MISSWRRRRATRRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETW 60
           MISSWRRRRAT R  RG+  D  ELTIP+HFRCPISLD+MKDPVTLSTGITYDRASIETW
Sbjct: 1   MISSWRRRRATPRMPRGSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETW 60

Query: 61  IEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDI 120
           IEGGNF+CP TNQPLQT+DSIPNHNIRKMIQDWCVEN+AYG+ERIPTPRVP SP Q+RDI
Sbjct: 61  IEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDI 120

Query: 121 LEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLE 180
           LEK+TAAARRGD +GCKS VEKIK L KESERNKKCI+ NG  +  SS F+AFS  E  E
Sbjct: 121 LEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFE 180

Query: 181 KSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIIS 240
           K IEILEE++SAM ++FPLQEESIK+LKS+ SLQ+L  F+KGG +S RRNS+L+LK+IIS
Sbjct: 181 KRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIIS 240

Query: 241 SYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIA---SSSNPQRFTKALL 300
           SYP+K  +LG IQGALEGL+KLIK+PICSSSKKASLFITYH+IA   SSSN  RFTKALL
Sbjct: 241 SYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALL 300

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG VSLLLETLVDAERSVCERALGAFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 360

Query: 361 ATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420
           ATELSVSIVWKLVK+ES+EE+EDG ++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 ATELSVSIVWKLVKNESKEEKEDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 421 LLNNLHRDSLECIDSLDFKGIKRPF 443
           LLNNLHRD LECIDSLDFK +KR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of Carg27436 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.4e-197
Identity = 356/447 (79.64%), Postives = 399/447 (89.26%), Query Frame = 0

Query: 1   MISSWRRRRATRRTAR---GTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASI 60
           M+ SWRRRRATRR AR   G G  M ELTIPSHF CPISLD+MKDPVT+STGITYDRA+I
Sbjct: 2   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 61

Query: 61  ETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQI 120
           ETWIEGGNFSCP TNQPL T+DSIPNHNIRKMIQ WCVEN+A+G+ERIPTPRVP SPVQ+
Sbjct: 62  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQV 121

Query: 121 RDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQE 180
           R+IL+KI AA RR D +GCKS VEKIK+L +ESERNKKCI+ NG  +V+SSAF+AFSK E
Sbjct: 122 REILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 181

Query: 181 PLEKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQ 240
             EK +EILEE++S MA++FPLQEE+IK+LKS+AS+QALV F++G   SRRR+SIL+LK+
Sbjct: 182 RFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 241

Query: 241 IISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL 300
           IISSYP+K ++LGEIQG LEGL+KLIKEPICSSSKKASLFITYH IASSSN + FTKA L
Sbjct: 242 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFL 301

Query: 301 EMGFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360
           +MG V LLLETLVDAERSVCERALG FDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 302 QMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361

Query: 361 ATELSVSIVWKLVKHES--EEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 420
            TELSVSIVWKLVKHES  EEE+EDG VKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL
Sbjct: 362 VTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 421

Query: 421 LKLLNNLHRDSLECIDSLDFKGIKRPF 443
           LKLLNNLHR+ LECIDSLDFK +KR F
Sbjct: 422 LKLLNNLHRERLECIDSLDFKDLKRTF 448

BLAST of Carg27436 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-99
Identity = 198/420 (47.14%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 24  ELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPN 83
           E+TIP  F+CPIS+D+MKDPV +STGITYDR SIETWI  GN +CP+TN  L T D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 84  HNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKI 143
           H IRKMIQ WCVE  +  ++RIPTPRVP+ P ++ +I  K+++A RRGD + C   +EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 144 KMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPLQEES 203
           K L  ESE+N+KC+  N    V+   F  FS     EK   +L E++S +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 204 IKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLI 263
           I  L S  S + +   +K    S R+N+  I+K+I+S    +        G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 264 KEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCERALGA 323
           ++ + SSS K+SL   Y ++             LE+G VS+ +E +VDAE SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQK---PEIASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 324 FDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRV 383
            D ICE++ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    ED   
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED--- 387

Query: 384 KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSL-DFKGIKRPF 443
              A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK IK+PF
Sbjct: 388 ---AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Carg27436 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 313.2 bits (801), Expect = 3.4e-85
Identity = 177/427 (41.45%), Postives = 262/427 (61.36%), Query Frame = 0

Query: 20  SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLD 79
           S+  E+TIPS F+CPIS ++MKDPV +++GITYDR +IE W E G  +CP+TN  L +L+
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 80  SIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSS 139
            IPNH IR+MIQ WC  +   G+ERIPTPRVPV+  Q+ +I E+++AA RRGD   C   
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 140 VEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL 199
           V K+  L KESERN+KC+  NGA  V+   F AFS+      +  +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSEN---ANASLLLEETVSVLTWMLPI 205

Query: 200 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGL 259
             E    L + +S   LV  ++ G     +N+  ++K+++         L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNG----DQNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 260 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCER 319
           +K I      S+   SL   +H+I ++   Q      LE+  V++ +E LVD+E SVCE+
Sbjct: 266 MKSINR---DSTCVNSLISIHHMILTN---QETVSRFLELDLVNITVEMLVDSENSVCEK 325

Query: 320 ALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEE 379
           AL   + ICE+KEGRE+   + L +P++VKKIL++S+   +  VS++WK+ K        
Sbjct: 326 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK------SG 385

Query: 380 DGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS----LECIDSLDF 439
           DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + + +         S++F
Sbjct: 386 DGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEF 431

Query: 440 KGIKRPF 443
           K +K+PF
Sbjct: 446 KHVKKPF 431

BLAST of Carg27436 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 283.9 bits (725), Expect = 2.2e-76
Identity = 168/427 (39.34%), Postives = 249/427 (58.31%), Query Frame = 0

Query: 20  SDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLD 79
           S+  E+TIPS F+CPIS ++MKDP                W E G  +CP+TN  L +L+
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLE 85

Query: 80  SIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSS 139
            IPNH IR+MIQ WC  +   G+ERIPTPRVPV+  Q+ +I E+++AA RRGD   C   
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 140 VEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL 199
           V K+  L KESERN+KC+  NGA  V+   F AFS+      +  +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSEN---ANASLLLEETVSVLTWMLPI 205

Query: 200 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGL 259
             E    L + +S   LV  ++ G     +N+  ++K+++         L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNG----DQNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 260 VKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETLVDAERSVCER 319
           +K I      S+   SL   +H+I ++   Q      LE+  V++ +E LVD+E SVCE+
Sbjct: 266 MKSINR---DSTCVNSLISIHHMILTN---QETVSRFLELDLVNITVEMLVDSENSVCEK 325

Query: 320 ALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEE 379
           AL   + ICE+KEGRE+   + L +P++VKKIL++S+   +  VS++WK+ K        
Sbjct: 326 ALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK------SG 385

Query: 380 DGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDS----LECIDSLDF 439
           DG    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + + +         S++F
Sbjct: 386 DGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEF 415

Query: 440 KGIKRPF 443
           K +K+PF
Sbjct: 446 KHVKKPF 415

BLAST of Carg27436 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-61
Identity = 139/346 (40.17%), Postives = 222/346 (64.16%), Query Frame = 0

Query: 102 VERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNG 161
           +ERI +PRV ++P    +I  ++  AA R +   C   V KIK L +  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 162 ASHVISSAFQAFSKQEPLEKSIEILEEMISAMAIMFPL-QEESIKNLKSKASLQALVLFM 221
           +   +SS F+ F+     +  + +LEE++  ++   PL + E    + S ASL  LV F+
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 222 KGGVLSRRRNSILILKQIISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITY 281
            G     R+N+   ++++I+   +    L +++GA EGLVK+I++ + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 282 HIIASSSNPQRFTKALLEMGFVSLLLETLV-DAERSVCERALGAFDGICESKEGREEAYA 341
             I+S+    + T+  +++G V L+ E +V +AE+SVCER+L   +  C++++G+E+   
Sbjct: 220 RAISSN---DKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLR 279

Query: 342 HALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLL 401
           +AL +P++VKKILRVSDLAT+ SVSI+WKL K++ + E +D R+ VEALQVGAF+KLL+L
Sbjct: 280 NALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDD-RLLVEALQVGAFEKLLVL 339

Query: 402 LQVGCSEWTKEKATELLKLLNNLHR--DSLECID-SLDFKGIKRPF 443
           LQVGC + TKEKA+ELL+ LN      +   C+D S+  K +K+ F
Sbjct: 340 LQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of Carg27436 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-55
Identity = 144/419 (34.37%), Postives = 223/419 (53.22%), Query Frame = 0

Query: 25  LTIPSHFRCPISLDMMKDPVTLSTGITYDRASIETWIEGGNFSCPITNQPLQTLDSIPNH 84
           + IP HFRCPISLD+M DPVT+STG TYDR SI++WI  GN +CP+T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 85  NIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRDILEKITAAARRGDGDGCKS---SVE 144
            +R++IQ+WCV N++ GVERIPTP+ P  P+ +R +L +  A+A  G     +S   ++ 
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQ--ASAITGTHVSVRSRAAAIR 131

Query: 145 KIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPLEKSIEILEEMISAMAI--MFPL 204
           +++ LA++SE+N+  I G+ A  ++      F+  E    S E++ E ++ + +  M   
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTET 191

Query: 205 QEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQIISSYPQKATKL--GEIQGALE 264
           + E++ +  S+      +LF     +  R N+  +++ +++       KL         E
Sbjct: 192 ECEAVASDPSRVGFMTRLLF--DSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 265 GLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEMGFVSLLLETL-VDAERSV 324
           G++ L+K PI   S + +L I    I +    ++     +  G   +L++ L  D +R  
Sbjct: 252 GVLDLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD 311

Query: 325 CERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESEE 384
            ER L   + +C   EG      HALT+P++VK ILRVSD ATE +   +  L   E   
Sbjct: 312 TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEE-- 371

Query: 385 EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDSLECIDSLDF 436
                R + EA   G   +LLLL+Q  C+E  K KA  LLKLL +   D    + S DF
Sbjct: 372 -----RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDD-STVHSDDF 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030606.12.6e-244100.00U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6599923.18.4e-24399.55U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023512745.12.3e-24098.64U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
XP_022942705.18.2e-23897.96U-box domain-containing protein 21-like [Cucurbita moschata][more]
XP_022995728.14.6e-23395.70U-box domain-containing protein 21-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5PNY61.6e-9847.14U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D14.9e-8441.45U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q9FXA44.0e-5434.37U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9LT791.3e-5233.73U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q84TG34.6e-5032.60E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FWS83.9e-23897.96RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447660... [more]
A0A6J1K8U92.2e-23395.70RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 P... [more]
A0A0A0KMI82.0e-20281.80RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
A0A1S3CPH47.8e-20281.35RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ... [more]
A0A6J1FPJ64.4e-19779.64RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642... [more]
Match NameE-valueIdentityDescription
AT5G37490.11.1e-9947.14ARM repeat superfamily protein [more]
AT1G66160.13.4e-8541.45CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.22.2e-7639.34CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.11.2e-6140.17ARM repeat superfamily protein [more]
AT1G49780.12.8e-5534.37plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 30..93
e-value: 4.2E-28
score: 109.4
IPR003613U box domainPFAMPF04564U-boxcoord: 27..98
e-value: 2.8E-19
score: 69.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 26..100
score: 40.010868
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 5..104
e-value: 1.6E-28
score: 100.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 117..422
e-value: 6.9E-14
score: 53.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR22849:SF139ARM REPEAT SUPERFAMILY PROTEINcoord: 9..442
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 9..442
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 31..73
e-value: 3.49643E-24
score: 92.2966
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 18..103
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 118..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27436-RACarg27436-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity