Carg27429 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27429
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPentatricopeptide repeat-containing protein
LocationCarg_Chr09: 9369061 .. 9370509 (-)
RNA-Seq ExpressionCarg27429
SyntenyCarg27429
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGCAACACCTTCTTCGTCCCTGCAACTATAAGACTCTAGAAACTGTTGCTGTTCATCTTGCCCCCAAAACTCCATTGCTTCACAACTCAATCTCCTCATCATCCTCTCTTTACCAACCAGACTTAAATGTTCATAACGAATTGAAAACTCTGAGTGCCACCAACATAAACCATAAACACCTTGAACAGCAACCAGATTTCTCAATTGGGTCTCCATGTAGGGTCCAGAAACTCATTGCTTCCCAATCTGATCCTCTTCTTGCGAAGGAAATTTTCGATTATGCTTGTCGTCAACCCCATTTTCGCCCATCGTCTTCCTCTTTTCTCGTTCTTATCCTCAAGCTAGGGCGTTCCAAGTACTTCTCTCTTATTGATGATCTTCTTCTTAGCTTCAAGTCTAGAGGCTACCCTCTCTCTCCAACTGTTTTCTCCTACATAATCAAAATCTATGGTGAAGCTGATTTACCAGATAAAGCTCTCAAAACCTTTTATACTATGATCGAGTTTGGCTGTACGCCATCGTCCAAACAGTTGAACCGCATACTAGAAATTTTGGTTTCTCACCGTAACTTCATTCGGCCAGCTTTTGATCTTTTCAAGAATGCCCGTCATCATGGTGTGTTGCCCAACACCAAGTCTTACAACATCCTCATGCGTGCATTCTGTTGGAATGGAGATCTTAGCATTGCCTACACTCTGTTCAACAAAATGTTCAAACGAGATGTCATTCCAGATGTCGAGTCGTATCGGATATTAATGCAGGGTCTATGCAGGAAGAATCAAGTGATTGGTGCCGTCGACTTGCTAGAAGATATGTTAAACAAAGGCTACGTTCCAGACACATTGAGCTATGCCACTTTGTTAAATAGTTTATGTAGGAAGAAAAAGCTGAGAGAAGCTTATAAACTTCTCTGTAGAATGAAGGTTAAGGGTTGCAATCCTGATGTTGCTCATTACAATACAGTTATAACGGGATTTTGCAGAGAAGGGCGCGCTCTCGATGCTTGTAAGATTTTGGAGGATATGCAGTCGAACCGTTGTTTGCCTAACTTAGTGTCTTACCAGAGCTTGACTAATGGATTATGTGATCAAGGAATGTTTGAATTGGCAAAGGATTATGTTGAGGAGATGTCATTAAAGGGTTTTTGCCCACATTTCTCTGTCATTCATACTTTGGTAAAGGGTTTCATTAACGTTGGCAGAATCGACGATTCGTGTAGCGTTCTTGAAGACATACTAAAGCATGGGAAAGCCCCTCATTCTGAAACTTGGGAGATGATTCTATCTGGAATTTGTGAAGTTGAGGACACTGTAAAGTTATGTGAAATTTTAGAGAAGATTTTGAAGAAAGATGTAAGAAGAGACACTAGAATAGTGGAAGCAGGCTCTGGGTTGGGTGAGTATTTGATTAGGAAGTTACAAGCCTCCAAATCCCGAAGGGCTTGA

mRNA sequence

ATGCGGCAACACCTTCTTCGTCCCTGCAACTATAAGACTCTAGAAACTGTTGCTGTTCATCTTGCCCCCAAAACTCCATTGCTTCACAACTCAATCTCCTCATCATCCTCTCTTTACCAACCAGACTTAAATGTTCATAACGAATTGAAAACTCTGAGTGCCACCAACATAAACCATAAACACCTTGAACAGCAACCAGATTTCTCAATTGGGTCTCCATGTAGGGTCCAGAAACTCATTGCTTCCCAATCTGATCCTCTTCTTGCGAAGGAAATTTTCGATTATGCTTGTCGTCAACCCCATTTTCGCCCATCGTCTTCCTCTTTTCTCGTTCTTATCCTCAAGCTAGGGCGTTCCAAGTACTTCTCTCTTATTGATGATCTTCTTCTTAGCTTCAAGTCTAGAGGCTACCCTCTCTCTCCAACTGTTTTCTCCTACATAATCAAAATCTATGGTGAAGCTGATTTACCAGATAAAGCTCTCAAAACCTTTTATACTATGATCGAGTTTGGCTGTACGCCATCGTCCAAACAGTTGAACCGCATACTAGAAATTTTGGTTTCTCACCGTAACTTCATTCGGCCAGCTTTTGATCTTTTCAAGAATGCCCGTCATCATGGTGTGTTGCCCAACACCAAGTCTTACAACATCCTCATGCGTGCATTCTGTTGGAATGGAGATCTTAGCATTGCCTACACTCTGTTCAACAAAATGTTCAAACGAGATGTCATTCCAGATGTCGAGTCGTATCGGATATTAATGCAGGGTCTATGCAGGAAGAATCAAGTGATTGGTGCCGTCGACTTGCTAGAAGATATGTTAAACAAAGGCTACGTTCCAGACACATTGAGCTATGCCACTTTGTTAAATAGTTTATGTAGGAAGAAAAAGCTGAGAGAAGCTTATAAACTTCTCTGTAGAATGAAGGTTAAGGGTTGCAATCCTGATGTTGCTCATTACAATACAGTTATAACGGGATTTTGCAGAGAAGGGCGCGCTCTCGATGCTTGTAAGATTTTGGAGGATATGCAGTCGAACCGTTGTTTGCCTAACTTAGTGTCTTACCAGAGCTTGACTAATGGATTATGTGATCAAGGAATGTTTGAATTGGCAAAGGATTATGTTGAGGAGATGTCATTAAAGGGTTTTTGCCCACATTTCTCTGTCATTCATACTTTGGTAAAGGGTTTCATTAACGTTGGCAGAATCGACGATTCGTGTAGCGTTCTTGAAGACATACTAAAGCATGGGAAAGCCCCTCATTCTGAAACTTGGGAGATGATTCTATCTGGAATTTGTGAAGTTGAGGACACTGTAAAGTTATGTGAAATTTTAGAGAAGATTTTGAAGAAAGATGTAAGAAGAGACACTAGAATAGTGGAAGCAGGCTCTGGGTTGGGTGAGTATTTGATTAGGAAGTTACAAGCCTCCAAATCCCGAAGGGCTTGA

Coding sequence (CDS)

ATGCGGCAACACCTTCTTCGTCCCTGCAACTATAAGACTCTAGAAACTGTTGCTGTTCATCTTGCCCCCAAAACTCCATTGCTTCACAACTCAATCTCCTCATCATCCTCTCTTTACCAACCAGACTTAAATGTTCATAACGAATTGAAAACTCTGAGTGCCACCAACATAAACCATAAACACCTTGAACAGCAACCAGATTTCTCAATTGGGTCTCCATGTAGGGTCCAGAAACTCATTGCTTCCCAATCTGATCCTCTTCTTGCGAAGGAAATTTTCGATTATGCTTGTCGTCAACCCCATTTTCGCCCATCGTCTTCCTCTTTTCTCGTTCTTATCCTCAAGCTAGGGCGTTCCAAGTACTTCTCTCTTATTGATGATCTTCTTCTTAGCTTCAAGTCTAGAGGCTACCCTCTCTCTCCAACTGTTTTCTCCTACATAATCAAAATCTATGGTGAAGCTGATTTACCAGATAAAGCTCTCAAAACCTTTTATACTATGATCGAGTTTGGCTGTACGCCATCGTCCAAACAGTTGAACCGCATACTAGAAATTTTGGTTTCTCACCGTAACTTCATTCGGCCAGCTTTTGATCTTTTCAAGAATGCCCGTCATCATGGTGTGTTGCCCAACACCAAGTCTTACAACATCCTCATGCGTGCATTCTGTTGGAATGGAGATCTTAGCATTGCCTACACTCTGTTCAACAAAATGTTCAAACGAGATGTCATTCCAGATGTCGAGTCGTATCGGATATTAATGCAGGGTCTATGCAGGAAGAATCAAGTGATTGGTGCCGTCGACTTGCTAGAAGATATGTTAAACAAAGGCTACGTTCCAGACACATTGAGCTATGCCACTTTGTTAAATAGTTTATGTAGGAAGAAAAAGCTGAGAGAAGCTTATAAACTTCTCTGTAGAATGAAGGTTAAGGGTTGCAATCCTGATGTTGCTCATTACAATACAGTTATAACGGGATTTTGCAGAGAAGGGCGCGCTCTCGATGCTTGTAAGATTTTGGAGGATATGCAGTCGAACCGTTGTTTGCCTAACTTAGTGTCTTACCAGAGCTTGACTAATGGATTATGTGATCAAGGAATGTTTGAATTGGCAAAGGATTATGTTGAGGAGATGTCATTAAAGGGTTTTTGCCCACATTTCTCTGTCATTCATACTTTGGTAAAGGGTTTCATTAACGTTGGCAGAATCGACGATTCGTGTAGCGTTCTTGAAGACATACTAAAGCATGGGAAAGCCCCTCATTCTGAAACTTGGGAGATGATTCTATCTGGAATTTGTGAAGTTGAGGACACTGTAAAGTTATGTGAAATTTTAGAGAAGATTTTGAAGAAAGATGTAAGAAGAGACACTAGAATAGTGGAAGCAGGCTCTGGGTTGGGTGAGTATTTGATTAGGAAGTTACAAGCCTCCAAATCCCGAAGGGCTTGA

Protein sequence

MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHKHLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSKYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFKRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSRRA
Homology
BLAST of Carg27429 vs. NCBI nr
Match: KAG7025108.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 979.9 bits (2532), Expect = 7.7e-282
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK
Sbjct: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. NCBI nr
Match: KAG6592265.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 975.3 bits (2520), Expect = 1.9e-280
Identity = 479/482 (99.38%), Postives = 481/482 (99.79%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK
Sbjct: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSN CLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNGCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWE++LSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEIVLSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. NCBI nr
Match: XP_022932826.1 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucurbita moschata] >XP_022932827.1 pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 974.5 bits (2518), Expect = 3.2e-280
Identity = 476/482 (98.76%), Postives = 481/482 (99.79%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK
Sbjct: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMR FCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRVFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDV+PDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVVPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEM+LKGFCPHFSVIHTLVKGFINVGRIDDSCSVLED+LKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMTLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDMLKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWEMI+SG+CEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEMIISGVCEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. NCBI nr
Match: XP_023515125.1 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.4 bits (2489), Expect = 7.4e-277
Identity = 470/482 (97.51%), Postives = 479/482 (99.38%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKT+ETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTL+ATNINHK
Sbjct: 1   MRQHLLRPCNYKTIETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLNATNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
            LE+QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  RLEEQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLIDDLLLSFKSRGYP SPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLIDDLLLSFKSRGYPFSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDV+PDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVVPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEM+LKGFCPHFSVIHTLVKGFINVGRIDDSCSVLED+LK GKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMTLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDMLKDGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWE+++SGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEIVISGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. NCBI nr
Match: XP_022974029.1 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucurbita maxima] >XP_022974030.1 pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 958.7 bits (2477), Expect = 1.8e-275
Identity = 468/482 (97.10%), Postives = 478/482 (99.17%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKT+ETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTL+ TNINHK
Sbjct: 1   MRQHLLRPCNYKTIETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLNDTNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLE+QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEEQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLI+DLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLINDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHR+FIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRDFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGY+PDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYIPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQ N CLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQLNGCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEM+L GFCPHFSVIHTLVKGFINVGRIDDSCSVLED+LKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMTLNGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDMLKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWE+I+SGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEIIISGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. ExPASy Swiss-Prot
Match: Q8LDU5 (Pentatricopeptide repeat-containing protein At4g01400, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g01400 PE=2 SV=2)

HSP 1 Score: 558.5 bits (1438), Expect = 7.2e-158
Identity = 261/419 (62.29%), Postives = 333/419 (79.47%), Query Frame = 0

Query: 60  KHLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRS 119
           K +   P   IGSP RVQKLIASQSDPLLAKEIFDYA +QP+FR S SS L+LILKLGR 
Sbjct: 38  KPIVSNPKSPIGSPTRVQKLIASQSDPLLAKEIFDYASQQPNFRHSRSSHLILILKLGRG 97

Query: 120 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 179
           +YF+LIDD+L   +S GYPL+  +F+Y+IK+Y EA LP+K L TFY M+EF  TP  K L
Sbjct: 98  RYFNLIDDVLAKHRSSGYPLTGEIFTYLIKVYAEAKLPEKVLSTFYKMLEFNFTPQPKHL 157

Query: 180 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 239
           NRIL++LVSHR +++ AF+LFK++R HGV+PNT+SYN+LM+AFC N DLSIAY LF KM 
Sbjct: 158 NRILDVLVSHRGYLQKAFELFKSSRLHGVMPNTRSYNLLMQAFCLNDDLSIAYQLFGKML 217

Query: 240 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 299
           +RDV+PDV+SY+IL+QG CRK QV GA++LL+DMLNKG+VPD LSY TLLNSLCRK +LR
Sbjct: 218 ERDVVPDVDSYKILIQGFCRKGQVNGAMELLDDMLNKGFVPDRLSYTTLLNSLCRKTQLR 277

Query: 300 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 359
           EAYKLLCRMK+KGCNPD+ HYNT+I GFCRE RA+DA K+L+DM SN C PN VSY++L 
Sbjct: 278 EAYKLLCRMKLKGCNPDLVHYNTMILGFCREDRAMDARKVLDDMLSNGCSPNSVSYRTLI 337

Query: 360 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 419
            GLCDQGMF+  K Y+EEM  KGF PHFSV + LVKGF + G+++++C V+E ++K+G+ 
Sbjct: 338 GGLCDQGMFDEGKKYLEEMISKGFSPHFSVSNCLVKGFCSFGKVEEACDVVEVVMKNGET 397

Query: 420 PHSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASK 479
            HS+TWEM++  IC  +++ K+   LE  +K+++  DTRIV+ G GLG YL  KLQ  +
Sbjct: 398 LHSDTWEMVIPLICNEDESEKIKLFLEDAVKEEITGDTRIVDVGIGLGSYLSSKLQMKR 456

BLAST of Carg27429 vs. ExPASy Swiss-Prot
Match: Q9FNL2 (Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX=3702 GN=At5g46100 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.3e-52
Identity = 113/372 (30.38%), Postives = 196/372 (52.69%), Query Frame = 0

Query: 72  SPCRVQKLIASQSDPLLAKEIFDYACRQ--PHFRPSSSSFLVLILKLGRSKYFSLIDDLL 131
           +P +V KL+ ++ D   +  +FD A  +    +    SSF  ++L+L  +  F   +DL+
Sbjct: 15  TPSQVIKLMRAEKDVEKSMAVFDSATAEYANGYVHDQSSFGYMVLRLVSANKFKAAEDLI 74

Query: 132 LSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRILEILVSH 191
           +  K     +S  +   I + YG    P  +L+ F+ M +F C PS K    +L ILV  
Sbjct: 75  VRMKIENCVVSEDILLSICRGYGRVHRPFDSLRVFHKMKDFDCDPSQKAYVTVLAILV-E 134

Query: 192 RNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWN-GDLSIAYTLFNKMFKRDVIPDVE 251
            N +  AF  +KN R  G+ P   S N+L++A C N G +     +F +M KR   PD  
Sbjct: 135 ENQLNLAFKFYKNMREIGLPPTVASLNVLIKALCRNDGTVDAGLKIFLEMPKRGCDPDSY 194

Query: 252 SYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRM 311
           +Y  L+ GLCR  ++  A  L  +M+ K   P  ++Y +L+N LC  K + EA + L  M
Sbjct: 195 TYGTLISGLCRFGRIDEAKKLFTEMVEKDCAPTVVTYTSLINGLCGSKNVDEAMRYLEEM 254

Query: 312 KVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLCDQGMF 371
           K KG  P+V  Y++++ G C++GR+L A ++ E M +  C PN+V+Y +L  GLC +   
Sbjct: 255 KSKGIEPNVFTYSSLMDGLCKDGRSLQAMELFEMMMARGCRPNMVTYTTLITGLCKEQKI 314

Query: 372 ELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSETWEM- 431
           + A + ++ M+L+G  P   +   ++ GF  + +  ++ + L++++  G  P+  TW + 
Sbjct: 315 QEAVELLDRMNLQGLKPDAGLYGKVISGFCAISKFREAANFLDEMILGGITPNRLTWNIH 374

Query: 432 ------ILSGIC 434
                 ++ G+C
Sbjct: 375 VKTSNEVVRGLC 385

BLAST of Carg27429 vs. ExPASy Swiss-Prot
Match: Q9FIX3 (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX=3702 GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.2e-47
Identity = 105/338 (31.07%), Postives = 180/338 (53.25%), Query Frame = 0

Query: 120 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 179
           +Y SL+   L       Y  S +VF  ++K Y    L DKAL   +     G  P     
Sbjct: 114 EYASLVFKSLQETYDLCYSTS-SVFDLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSY 173

Query: 180 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 239
           N +L+  +  +  I  A ++FK      V PN  +YNIL+R FC+ G++ +A TLF+KM 
Sbjct: 174 NAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKME 233

Query: 240 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 299
            +  +P+V +Y  L+ G C+  ++     LL  M  KG  P+ +SY  ++N LCR+ +++
Sbjct: 234 TKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMK 293

Query: 300 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 359
           E   +L  M  +G + D   YNT+I G+C+EG    A  +  +M  +   P++++Y SL 
Sbjct: 294 EVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLI 353

Query: 360 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 419
           + +C  G    A +++++M ++G CP+     TLV GF   G ++++  VL ++  +G +
Sbjct: 354 HSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFS 413

Query: 420 PHSETWEMILSGIC---EVEDTVKLCE-ILEKILKKDV 454
           P   T+  +++G C   ++ED + + E + EK L  DV
Sbjct: 414 PSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDV 450

BLAST of Carg27429 vs. ExPASy Swiss-Prot
Match: Q9M302 (Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana OX=3702 GN=At3g48810 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.2e-45
Identity = 116/447 (25.95%), Postives = 197/447 (44.07%), Query Frame = 0

Query: 55  TNINHKH-LEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLI 114
           TN+N  H L + P+ +      V K +  +S   LA   F        F+ +  +F V+I
Sbjct: 24  TNLNVNHLLTESPNHAEIKELDVVKRLRQESCVPLALHFFKSIANSNLFKHTPLTFEVMI 83

Query: 115 LKLGRSKYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCT 174
            KL        +  LL   K +G+  S  +F  +I +Y +  L ++A++ FY + EFGC 
Sbjct: 84  RKLAMDGQVDSVQYLLQQMKLQGFHCSEDLFISVISVYRQVGLAERAVEMFYRIKEFGCD 143

Query: 175 PSSKQLNRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYT 234
           PS K  N +L+ L+   N I+  + ++++ +  G  PN  +YN+L++A C N  +  A  
Sbjct: 144 PSVKIYNHVLDTLLG-ENRIQMIYMVYRDMKRDGFEPNVFTYNVLLKALCKNNKVDGAKK 203

Query: 235 LFNKMFKRDVIPD------------------------------VESYRILMQGLCRKNQV 294
           L  +M  +   PD                              V  Y  L+ GLC+++  
Sbjct: 204 LLVEMSNKGCCPDAVSYTTVISSMCEVGLVKEGRELAERFEPVVSVYNALINGLCKEHDY 263

Query: 295 IGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRMKVKGCN--------- 354
            GA +L+ +M+ KG  P+ +SY+TL+N LC   ++  A+  L +M  +GC+         
Sbjct: 264 KGAFELMREMVEKGISPNVISYSTLINVLCNSGQIELAFSFLTQMLKRGCHPNIYTLSSL 323

Query: 355 ---------------------------PDVAHYNTVITGFCREGRALDACKILEDMQSNR 414
                                      P+V  YNT++ GFC  G  + A  +   M+   
Sbjct: 324 VKGCFLRGTTFDALDLWNQMIRGFGLQPNVVAYNTLVQGFCSHGNIVKAVSVFSHMEEIG 383

Query: 415 CLPNLVSYQSLTNGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSC 435
           C PN+ +Y SL NG   +G  + A     +M   G CP+  V   +V+      +  ++ 
Sbjct: 384 CSPNIRTYGSLINGFAKRGSLDGAVYIWNKMLTSGCCPNVVVYTNMVEALCRHSKFKEAE 443

BLAST of Carg27429 vs. ExPASy Swiss-Prot
Match: Q9LQQ1 (Pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g07740 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 4.8e-45
Identity = 112/390 (28.72%), Postives = 197/390 (50.51%), Query Frame = 0

Query: 85  DPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSKYFSLIDDLLLSFKSRGYPLSPTVF 144
           DP  A  +F +  ++  FR    S+  LI KL +S+ F  +D +L   + R      ++F
Sbjct: 61  DPEEALSLF-HQYQEMGFRHDYPSYSSLIYKLAKSRNFDAVDQILRLVRYRNVRCRESLF 120

Query: 145 SYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRILEILVSHRNFIRPAFDLFKNAR 204
             +I+ YG+A   DKA+  F+ +  F C  + + LN ++ +LV +   +  A   F  A+
Sbjct: 121 MGLIQHYGKAGSVDKAIDVFHKITSFDCVRTIQSLNTLINVLVDNGE-LEKAKSFFDGAK 180

Query: 205 HHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFKRDVIPDVESYRILMQGLCRKNQVI 264
              + PN+ S+NIL++ F    D   A  +F++M + +V P V +Y  L+  LCR + + 
Sbjct: 181 DMRLRPNSVSFNILIKGFLDKCDWEAACKVFDEMLEMEVQPSVVTYNSLIGFLCRNDDMG 240

Query: 265 GAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRMKVKGCNPDVAHYNTVI 324
            A  LLEDM+ K   P+ +++  L+  LC K +  EA KL+  M+ +GC P + +Y  ++
Sbjct: 241 KAKSLLEDMIKKRIRPNAVTFGLLMKGLCCKGEYNEAKKLMFDMEYRGCKPGLVNYGILM 300

Query: 325 TGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLCDQGMFELAKDYVEEMSLKGFC 384
           +   + GR  +A  +L +M+  R  P++V Y  L N LC +     A   + EM +KG  
Sbjct: 301 SDLGKRGRIDEAKLLLGEMKKRRIKPDVVIYNILVNHLCTECRVPEAYRVLTEMQMKGCK 360

Query: 385 PHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSETWEMILSGICEVEDTVKLCEI 444
           P+ +    ++ GF  +   D   +VL  +L     P   T+  +++G+ +  +    C +
Sbjct: 361 PNAATYRMMIDGFCRIEDFDSGLNVLNAMLASRHCPTPATFVCMVAGLIKGGNLDHACFV 420

Query: 445 LEKILKKDVRRDTRIVEAGSGLGEYLIRKL 475
           LE + KK+       +  GSG  + L+  L
Sbjct: 421 LEVMGKKN-------LSFGSGAWQNLLSDL 441

BLAST of Carg27429 vs. ExPASy TrEMBL
Match: A0A6J1EXV3 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111439286 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.6e-280
Identity = 476/482 (98.76%), Postives = 481/482 (99.79%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK
Sbjct: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMR FCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRVFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDV+PDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVVPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEM+LKGFCPHFSVIHTLVKGFINVGRIDDSCSVLED+LKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMTLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDMLKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWEMI+SG+CEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEMIISGVCEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. ExPASy TrEMBL
Match: A0A6J1ICW5 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111472648 PE=4 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 8.9e-276
Identity = 468/482 (97.10%), Postives = 478/482 (99.17%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHK 60
           MRQHLLRPCNYKT+ETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTL+ TNINHK
Sbjct: 1   MRQHLLRPCNYKTIETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLNDTNINHK 60

Query: 61  HLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120
           HLE+QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK
Sbjct: 61  HLEEQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSK 120

Query: 121 YFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180
           YFSLI+DLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN
Sbjct: 121 YFSLINDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLN 180

Query: 181 RILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240
           RILEILVSHR+FIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK
Sbjct: 181 RILEILVSHRDFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFK 240

Query: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLRE 300
           RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGY+PDTLSYATLLNSLCRKKKLRE
Sbjct: 241 RDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYIPDTLSYATLLNSLCRKKKLRE 300

Query: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTN 360
           AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQ N CLPNLVSYQSLTN
Sbjct: 301 AYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQLNGCLPNLVSYQSLTN 360

Query: 361 GLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAP 420
           GLCDQGMFELAKDYVEEM+L GFCPHFSVIHTLVKGFINVGRIDDSCSVLED+LKHGKAP
Sbjct: 361 GLCDQGMFELAKDYVEEMTLNGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDMLKHGKAP 420

Query: 421 HSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480
           HSETWE+I+SGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR
Sbjct: 421 HSETWEIIISGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSR 480

Query: 481 RA 483
           RA
Sbjct: 481 RA 482

BLAST of Carg27429 vs. ExPASy TrEMBL
Match: A0A5A7SWW3 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold155G00670 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.6e-251
Identity = 428/478 (89.54%), Postives = 450/478 (94.14%), Query Frame = 0

Query: 4   HLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHKHLE 63
           HLLRP NY+T+ETVA H+A   PLLHN ISSSSSLYQP LNVHNE KTL  TNINHK  E
Sbjct: 86  HLLRPGNYRTIETVAAHVARNAPLLHNLISSSSSLYQPHLNVHNESKTL-ITNINHKQCE 145

Query: 64  QQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSKYFS 123
            QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSS LVLILKLGRSKYFS
Sbjct: 146 DQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSLLVLILKLGRSKYFS 205

Query: 124 LIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRIL 183
           LIDDLLLSFKSRGYP++PT FSYIIKIYGEADLPDKALK FYTMIEFGCTPSSKQLNRIL
Sbjct: 206 LIDDLLLSFKSRGYPVTPTAFSYIIKIYGEADLPDKALKVFYTMIEFGCTPSSKQLNRIL 265

Query: 184 EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFKRDV 243
           EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNIL+RAFCWNG++SIAY LFNKMF+ DV
Sbjct: 266 EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILIRAFCWNGNISIAYKLFNKMFEGDV 325

Query: 244 IPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYK 303
           IPDVE+YR LMQGLCRKNQV GAVDLLEDMLNKGY+PDTLSYATLLNSLCRKKKL+EAYK
Sbjct: 326 IPDVETYRTLMQGLCRKNQVNGAVDLLEDMLNKGYIPDTLSYATLLNSLCRKKKLKEAYK 385

Query: 304 LLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLC 363
           LLCRMKVKGCNPD+AHYNTVI GFCREGRALDACKILEDMQSN CLPNLVSY+SLTNGLC
Sbjct: 386 LLCRMKVKGCNPDIAHYNTVIMGFCREGRALDACKILEDMQSNGCLPNLVSYESLTNGLC 445

Query: 364 DQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSE 423
           DQGMFELAK YVEEM+LKGF PHFSVIH LVKGF NVGR+D+SCSVLE +LKHGKAPHS+
Sbjct: 446 DQGMFELAKGYVEEMTLKGFYPHFSVIHALVKGFHNVGRMDESCSVLEGMLKHGKAPHSD 505

Query: 424 TWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSRR 482
           TWE+I+SGICEVEDTVK CEILEKILKKDVRRDTRIVEAG+GLGEYLIRKLQASKSRR
Sbjct: 506 TWEIIISGICEVEDTVKFCEILEKILKKDVRRDTRIVEAGTGLGEYLIRKLQASKSRR 562

BLAST of Carg27429 vs. ExPASy TrEMBL
Match: A0A1S3CIG1 (pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103501324 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.6e-251
Identity = 428/478 (89.54%), Postives = 450/478 (94.14%), Query Frame = 0

Query: 4   HLLRPCNYKTLETVAVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINHKHLE 63
           HLLRP NY+T+ETVA H+A   PLLHN ISSSSSLYQP LNVHNE KTL  TNINHK  E
Sbjct: 4   HLLRPGNYRTIETVAAHVARNAPLLHNLISSSSSLYQPHLNVHNESKTL-ITNINHKQCE 63

Query: 64  QQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRSKYFS 123
            QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSS LVLILKLGRSKYFS
Sbjct: 64  DQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSLLVLILKLGRSKYFS 123

Query: 124 LIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRIL 183
           LIDDLLLSFKSRGYP++PT FSYIIKIYGEADLPDKALK FYTMIEFGCTPSSKQLNRIL
Sbjct: 124 LIDDLLLSFKSRGYPVTPTAFSYIIKIYGEADLPDKALKVFYTMIEFGCTPSSKQLNRIL 183

Query: 184 EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMFKRDV 243
           EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNIL+RAFCWNG++SIAY LFNKMF+ DV
Sbjct: 184 EILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILIRAFCWNGNISIAYKLFNKMFEGDV 243

Query: 244 IPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYK 303
           IPDVE+YR LMQGLCRKNQV GAVDLLEDMLNKGY+PDTLSYATLLNSLCRKKKL+EAYK
Sbjct: 244 IPDVETYRTLMQGLCRKNQVNGAVDLLEDMLNKGYIPDTLSYATLLNSLCRKKKLKEAYK 303

Query: 304 LLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLC 363
           LLCRMKVKGCNPD+AHYNTVI GFCREGRALDACKILEDMQSN CLPNLVSY+SLTNGLC
Sbjct: 304 LLCRMKVKGCNPDIAHYNTVIMGFCREGRALDACKILEDMQSNGCLPNLVSYESLTNGLC 363

Query: 364 DQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSE 423
           DQGMFELAK YVEEM+LKGF PHFSVIH LVKGF NVGR+D+SCSVLE +LKHGKAPHS+
Sbjct: 364 DQGMFELAKGYVEEMTLKGFYPHFSVIHALVKGFHNVGRMDESCSVLEGMLKHGKAPHSD 423

Query: 424 TWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKSRR 482
           TWE+I+SGICEVEDTVK CEILEKILKKDVRRDTRIVEAG+GLGEYLIRKLQASKSRR
Sbjct: 424 TWEIIISGICEVEDTVKFCEILEKILKKDVRRDTRIVEAGTGLGEYLIRKLQASKSRR 480

BLAST of Carg27429 vs. ExPASy TrEMBL
Match: A0A0A0K8U0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014780 PE=4 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 7.3e-246
Identity = 421/482 (87.34%), Postives = 449/482 (93.15%), Query Frame = 0

Query: 1   MRQHLLRPCNYKTLETV-AVHLAPKTPLLHNSISSSSSLYQPDLNVHNELKTLSATNINH 60
           M QHLLRPCNY+T+ETV A H+A K+PLL N ISSSSSLYQP LNVHNE K L  TN+ H
Sbjct: 1   MWQHLLRPCNYRTIETVAAAHVARKSPLLRNLISSSSSLYQPHLNVHNESKFL-ITNVKH 60

Query: 61  KHLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRS 120
           +  E QPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSS LVLILKLGRS
Sbjct: 61  EQCEDQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSLLVLILKLGRS 120

Query: 121 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 180
           KYFSLIDDLLLSFKSR YP++PT FSYIIKIYGEADLPDKALK FYTMI+FGCTPSSKQL
Sbjct: 121 KYFSLIDDLLLSFKSRRYPVTPTAFSYIIKIYGEADLPDKALKVFYTMIDFGCTPSSKQL 180

Query: 181 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 240
           NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNIL+RAFCWNG++SIAYTLFNKMF
Sbjct: 181 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILIRAFCWNGNISIAYTLFNKMF 240

Query: 241 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 300
           +R+VIPDVE+YR LMQGLCRKNQV GAVDLLEDMLNKGY+PDTLSYATLLNSLCRKKKLR
Sbjct: 241 ERNVIPDVETYRTLMQGLCRKNQVNGAVDLLEDMLNKGYIPDTLSYATLLNSLCRKKKLR 300

Query: 301 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 360
           EAYKLLCRMKVKGCNPD+AHYNTVI GFCREGRALDACKILEDMQSN CLPNLVSY+SLT
Sbjct: 301 EAYKLLCRMKVKGCNPDIAHYNTVIMGFCREGRALDACKILEDMQSNGCLPNLVSYESLT 360

Query: 361 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 420
           NGLCDQGMFELAK YVEEM+LKGF PHFSVIH LVKGF ++GRI +SCSVLED+LK GKA
Sbjct: 361 NGLCDQGMFELAKGYVEEMTLKGFYPHFSVIHALVKGFHSIGRIHESCSVLEDMLKRGKA 420

Query: 421 PHSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASKS 480
           PHS+TWE+I+SGICEVEDT K CE+ EKILKKDVRRDTRIVEAG+GLGEYLIRKLQAS S
Sbjct: 421 PHSDTWEIIISGICEVEDTAKFCEVWEKILKKDVRRDTRIVEAGTGLGEYLIRKLQASIS 480

Query: 481 RR 482
           RR
Sbjct: 481 RR 481

BLAST of Carg27429 vs. TAIR 10
Match: AT4G01400.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Pentatricopeptide repeat (PPR) superfamily protein (TAIR:AT5G46100.1); Has 40053 Blast hits to 12380 proteins in 263 species: Archae - 4; Bacteria - 27; Metazoa - 366; Fungi - 374; Plants - 38347; Viruses - 0; Other Eukaryotes - 935 (source: NCBI BLink). )

HSP 1 Score: 558.5 bits (1438), Expect = 5.1e-159
Identity = 261/419 (62.29%), Postives = 333/419 (79.47%), Query Frame = 0

Query: 60  KHLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRS 119
           K +   P   IGSP RVQKLIASQSDPLLAKEIFDYA +QP+FR S SS L+LILKLGR 
Sbjct: 38  KPIVSNPKSPIGSPTRVQKLIASQSDPLLAKEIFDYASQQPNFRHSRSSHLILILKLGRG 97

Query: 120 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 179
           +YF+LIDD+L   +S GYPL+  +F+Y+IK+Y EA LP+K L TFY M+EF  TP  K L
Sbjct: 98  RYFNLIDDVLAKHRSSGYPLTGEIFTYLIKVYAEAKLPEKVLSTFYKMLEFNFTPQPKHL 157

Query: 180 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 239
           NRIL++LVSHR +++ AF+LFK++R HGV+PNT+SYN+LM+AFC N DLSIAY LF KM 
Sbjct: 158 NRILDVLVSHRGYLQKAFELFKSSRLHGVMPNTRSYNLLMQAFCLNDDLSIAYQLFGKML 217

Query: 240 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 299
           +RDV+PDV+SY+IL+QG CRK QV GA++LL+DMLNKG+VPD LSY TLLNSLCRK +LR
Sbjct: 218 ERDVVPDVDSYKILIQGFCRKGQVNGAMELLDDMLNKGFVPDRLSYTTLLNSLCRKTQLR 277

Query: 300 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 359
           EAYKLLCRMK+KGCNPD+ HYNT+I GFCRE RA+DA K+L+DM SN C PN VSY++L 
Sbjct: 278 EAYKLLCRMKLKGCNPDLVHYNTMILGFCREDRAMDARKVLDDMLSNGCSPNSVSYRTLI 337

Query: 360 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 419
            GLCDQGMF+  K Y+EEM  KGF PHFSV + LVKGF + G+++++C V+E ++K+G+ 
Sbjct: 338 GGLCDQGMFDEGKKYLEEMISKGFSPHFSVSNCLVKGFCSFGKVEEACDVVEVVMKNGET 397

Query: 420 PHSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAGSGLGEYLIRKLQASK 479
            HS+TWEM++  IC  +++ K+   LE  +K+++  DTRIV+ G GLG YL  KLQ  +
Sbjct: 398 LHSDTWEMVIPLICNEDESEKIKLFLEDAVKEEITGDTRIVDVGIGLGSYLSSKLQMKR 456

BLAST of Carg27429 vs. TAIR 10
Match: AT4G01400.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: COG4 transport (InterPro:IPR013167), Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Pentatricopeptide repeat (PPR) superfamily protein (TAIR:AT5G46100.1); Has 26268 Blast hits to 8959 proteins in 289 species: Archae - 0; Bacteria - 3; Metazoa - 247; Fungi - 222; Plants - 25350; Viruses - 0; Other Eukaryotes - 446 (source: NCBI BLink). )

HSP 1 Score: 392.9 bits (1008), Expect = 3.7e-109
Identity = 197/404 (48.76%), Postives = 261/404 (64.60%), Query Frame = 0

Query: 60  KHLEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLILKLGRS 119
           K +   P   IGSP RVQKLIASQSDPLLAKEIFDYA +QP+FR S SS L+LILKLGR 
Sbjct: 38  KPIVSNPKSPIGSPTRVQKLIASQSDPLLAKEIFDYASQQPNFRHSRSSHLILILKLGRG 97

Query: 120 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 179
           +YF+LIDD+L   +S GYPL+  +F+Y+IK+Y EA LP+K L TFY M+EF  TP  K L
Sbjct: 98  RYFNLIDDVLAKHRSSGYPLTGEIFTYLIKVYAEAKLPEKVLSTFYKMLEFNFTPQPKHL 157

Query: 180 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 239
           NRIL++LVSHR +++ AF+LFK++R HGV+PNT+SYN+LM+AFC N DLSIAY LF KM 
Sbjct: 158 NRILDVLVSHRGYLQKAFELFKSSRLHGVMPNTRSYNLLMQAFCLNDDLSIAYQLFGKML 217

Query: 240 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 299
           +RDV+PDV+SY+IL+QG CRK QV GA++LL+DMLNKG+VPD                  
Sbjct: 218 ERDVVPDVDSYKILIQGFCRKGQVNGAMELLDDMLNKGFVPD------------------ 277

Query: 300 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 359
                                                                   ++L 
Sbjct: 278 --------------------------------------------------------RTLI 337

Query: 360 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 419
            GLCDQGMF+  K Y+EEM  KGF PHFSV + LVKGF + G+++++C V+E ++K+G+ 
Sbjct: 338 GGLCDQGMFDEGKKYLEEMISKGFSPHFSVSNCLVKGFCSFGKVEEACDVVEVVMKNGET 367

Query: 420 PHSETWEMILSGICEVEDTVKLCEILEKILKKDVRRDTRIVEAG 464
            HS+TWEM++  IC  +++ K+   LE  +K+++  DTRIV+ G
Sbjct: 398 LHSDTWEMVIPLICNEDESEKIKLFLEDAVKEEITGDTRIVDVG 367

BLAST of Carg27429 vs. TAIR 10
Match: AT5G46100.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 3.8e-53
Identity = 113/372 (30.38%), Postives = 196/372 (52.69%), Query Frame = 0

Query: 72  SPCRVQKLIASQSDPLLAKEIFDYACRQ--PHFRPSSSSFLVLILKLGRSKYFSLIDDLL 131
           +P +V KL+ ++ D   +  +FD A  +    +    SSF  ++L+L  +  F   +DL+
Sbjct: 15  TPSQVIKLMRAEKDVEKSMAVFDSATAEYANGYVHDQSSFGYMVLRLVSANKFKAAEDLI 74

Query: 132 LSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQLNRILEILVSH 191
           +  K     +S  +   I + YG    P  +L+ F+ M +F C PS K    +L ILV  
Sbjct: 75  VRMKIENCVVSEDILLSICRGYGRVHRPFDSLRVFHKMKDFDCDPSQKAYVTVLAILV-E 134

Query: 192 RNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWN-GDLSIAYTLFNKMFKRDVIPDVE 251
            N +  AF  +KN R  G+ P   S N+L++A C N G +     +F +M KR   PD  
Sbjct: 135 ENQLNLAFKFYKNMREIGLPPTVASLNVLIKALCRNDGTVDAGLKIFLEMPKRGCDPDSY 194

Query: 252 SYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRM 311
           +Y  L+ GLCR  ++  A  L  +M+ K   P  ++Y +L+N LC  K + EA + L  M
Sbjct: 195 TYGTLISGLCRFGRIDEAKKLFTEMVEKDCAPTVVTYTSLINGLCGSKNVDEAMRYLEEM 254

Query: 312 KVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLTNGLCDQGMF 371
           K KG  P+V  Y++++ G C++GR+L A ++ E M +  C PN+V+Y +L  GLC +   
Sbjct: 255 KSKGIEPNVFTYSSLMDGLCKDGRSLQAMELFEMMMARGCRPNMVTYTTLITGLCKEQKI 314

Query: 372 ELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKAPHSETWEM- 431
           + A + ++ M+L+G  P   +   ++ GF  + +  ++ + L++++  G  P+  TW + 
Sbjct: 315 QEAVELLDRMNLQGLKPDAGLYGKVISGFCAISKFREAANFLDEMILGGITPNRLTWNIH 374

Query: 432 ------ILSGIC 434
                 ++ G+C
Sbjct: 375 VKTSNEVVRGLC 385

BLAST of Carg27429 vs. TAIR 10
Match: AT5G39710.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 3.7e-48
Identity = 105/338 (31.07%), Postives = 180/338 (53.25%), Query Frame = 0

Query: 120 KYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCTPSSKQL 179
           +Y SL+   L       Y  S +VF  ++K Y    L DKAL   +     G  P     
Sbjct: 114 EYASLVFKSLQETYDLCYSTS-SVFDLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSY 173

Query: 180 NRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYTLFNKMF 239
           N +L+  +  +  I  A ++FK      V PN  +YNIL+R FC+ G++ +A TLF+KM 
Sbjct: 174 NAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKME 233

Query: 240 KRDVIPDVESYRILMQGLCRKNQVIGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLR 299
            +  +P+V +Y  L+ G C+  ++     LL  M  KG  P+ +SY  ++N LCR+ +++
Sbjct: 234 TKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMK 293

Query: 300 EAYKLLCRMKVKGCNPDVAHYNTVITGFCREGRALDACKILEDMQSNRCLPNLVSYQSLT 359
           E   +L  M  +G + D   YNT+I G+C+EG    A  +  +M  +   P++++Y SL 
Sbjct: 294 EVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLI 353

Query: 360 NGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSCSVLEDILKHGKA 419
           + +C  G    A +++++M ++G CP+     TLV GF   G ++++  VL ++  +G +
Sbjct: 354 HSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFS 413

Query: 420 PHSETWEMILSGIC---EVEDTVKLCE-ILEKILKKDV 454
           P   T+  +++G C   ++ED + + E + EK L  DV
Sbjct: 414 PSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDV 450

BLAST of Carg27429 vs. TAIR 10
Match: AT3G48810.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-46
Identity = 116/447 (25.95%), Postives = 197/447 (44.07%), Query Frame = 0

Query: 55  TNINHKH-LEQQPDFSIGSPCRVQKLIASQSDPLLAKEIFDYACRQPHFRPSSSSFLVLI 114
           TN+N  H L + P+ +      V K +  +S   LA   F        F+ +  +F V+I
Sbjct: 24  TNLNVNHLLTESPNHAEIKELDVVKRLRQESCVPLALHFFKSIANSNLFKHTPLTFEVMI 83

Query: 115 LKLGRSKYFSLIDDLLLSFKSRGYPLSPTVFSYIIKIYGEADLPDKALKTFYTMIEFGCT 174
            KL        +  LL   K +G+  S  +F  +I +Y +  L ++A++ FY + EFGC 
Sbjct: 84  RKLAMDGQVDSVQYLLQQMKLQGFHCSEDLFISVISVYRQVGLAERAVEMFYRIKEFGCD 143

Query: 175 PSSKQLNRILEILVSHRNFIRPAFDLFKNARHHGVLPNTKSYNILMRAFCWNGDLSIAYT 234
           PS K  N +L+ L+   N I+  + ++++ +  G  PN  +YN+L++A C N  +  A  
Sbjct: 144 PSVKIYNHVLDTLLG-ENRIQMIYMVYRDMKRDGFEPNVFTYNVLLKALCKNNKVDGAKK 203

Query: 235 LFNKMFKRDVIPD------------------------------VESYRILMQGLCRKNQV 294
           L  +M  +   PD                              V  Y  L+ GLC+++  
Sbjct: 204 LLVEMSNKGCCPDAVSYTTVISSMCEVGLVKEGRELAERFEPVVSVYNALINGLCKEHDY 263

Query: 295 IGAVDLLEDMLNKGYVPDTLSYATLLNSLCRKKKLREAYKLLCRMKVKGCN--------- 354
            GA +L+ +M+ KG  P+ +SY+TL+N LC   ++  A+  L +M  +GC+         
Sbjct: 264 KGAFELMREMVEKGISPNVISYSTLINVLCNSGQIELAFSFLTQMLKRGCHPNIYTLSSL 323

Query: 355 ---------------------------PDVAHYNTVITGFCREGRALDACKILEDMQSNR 414
                                      P+V  YNT++ GFC  G  + A  +   M+   
Sbjct: 324 VKGCFLRGTTFDALDLWNQMIRGFGLQPNVVAYNTLVQGFCSHGNIVKAVSVFSHMEEIG 383

Query: 415 CLPNLVSYQSLTNGLCDQGMFELAKDYVEEMSLKGFCPHFSVIHTLVKGFINVGRIDDSC 435
           C PN+ +Y SL NG   +G  + A     +M   G CP+  V   +V+      +  ++ 
Sbjct: 384 CSPNIRTYGSLINGFAKRGSLDGAVYIWNKMLTSGCCPNVVVYTNMVEALCRHSKFKEAE 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025108.17.7e-282100.00Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
KAG6592265.11.9e-28099.38Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022932826.13.2e-28098.76pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucur... [more]
XP_023515125.17.4e-27797.51pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucur... [more]
XP_022974029.11.8e-27597.10pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like [Cucur... [more]
Match NameE-valueIdentityDescription
Q8LDU57.2e-15862.29Pentatricopeptide repeat-containing protein At4g01400, mitochondrial OS=Arabidop... [more]
Q9FNL25.3e-5230.38Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX... [more]
Q9FIX35.2e-4731.07Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX... [more]
Q9M3022.2e-4525.95Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana OX... [more]
Q9LQQ14.8e-4528.72Pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1EXV31.6e-28098.76pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cuc... [more]
A0A6J1ICW58.9e-27697.10pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cuc... [more]
A0A5A7SWW37.6e-25189.54Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CIG17.6e-25189.54pentatricopeptide repeat-containing protein At4g01400, mitochondrial-like OS=Cuc... [more]
A0A0A0K8U07.3e-24687.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014780 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G01400.35.1e-15962.29FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G01400.13.7e-10948.76FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT5G46100.13.8e-5330.38Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G39710.13.7e-4831.07Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G48810.11.5e-4625.95Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 298..468
e-value: 2.1E-33
score: 118.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 53..190
e-value: 3.9E-12
score: 47.9
coord: 191..297
e-value: 1.2E-28
score: 101.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 214..247
e-value: 1.0E-5
score: 23.4
coord: 353..385
e-value: 7.7E-6
score: 23.8
coord: 320..351
e-value: 1.2E-8
score: 32.6
coord: 143..175
e-value: 2.0E-4
score: 19.3
coord: 284..317
e-value: 1.7E-8
score: 32.1
coord: 249..282
e-value: 8.9E-7
score: 26.7
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 210..259
e-value: 7.6E-15
score: 54.9
coord: 315..364
e-value: 9.1E-14
score: 51.4
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 276..309
e-value: 5.3E-9
score: 35.7
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 143..172
e-value: 0.014
score: 15.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 211..245
score: 11.443655
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 351..385
score: 10.785976
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 316..350
score: 11.838262
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 246..280
score: 11.465577
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 281..315
score: 12.232868
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 140..174
score: 9.371969
NoneNo IPR availablePANTHERPTHR47942:SF2OS09G0532800 PROTEINcoord: 56..473
NoneNo IPR availablePANTHERPTHR47942TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 56..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27429-RACarg27429-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
biological_process GO:0000963 mitochondrial RNA processing
biological_process GO:0015031 protein transport
biological_process GO:0060628 regulation of ER to Golgi vesicle-mediated transport
biological_process GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
cellular_component GO:0070939 Dsl1/NZR complex
cellular_component GO:0016020 membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding