Carg27408 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27408
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontobamovirus multiplication protein 2A
LocationCarg_Chr19: 5006087 .. 5009057 (+)
RNA-Seq ExpressionCarg27408
SyntenyCarg27408
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTTTTTAGGGATCGAAAGCGTATAGAGGATGGCCTGCAGAGGGTGCTTTGAGTGCCTGTTGAAGCTTCTGAACTTCTTCTTATCCCTTCTGGGTCTTGCCATGGTGGGGTATGGGATTTACTTGTTGGTTGAGTACTTGCAATCTCCTAGTGATATCCCAAGACCTCCGTTGAGTGGTGATCACGATCTGGTCCAGCTTGGTCGACCAATGCTAATGGCCGTGTCTCTGTCTTCTAACATCTTTGATCACCTTCCAAAAGCCTGGTATGACTTTTCCTTTTAAATATAATATGTATGTTTCTTACTGCTTGTAATGGCTGACTCTAGGCAGAACAAATATTCTGAAAAATAAAGGATATTGAGCTTCATAGGTAACTCATTGATTTGGTGGTCATGTTTCCTACCCCTAGGTCAGAAGTTCGATCCCCATCGTGCCCTTCCCCCCAATTCCTGTTGTAAGAATAAGATAACCTCTCAATTTTTTAGAAAAAAAAGAAAAGAAAAGAAAAGAAAAAGAAGGAATGAGAGGTACATGATTAACGTCATGATGTGATGAAGTTTATGAAGCAGCTGAATTATGGATTCACTCGTTGAAGATAAAAAATTTATGAATTTCCTAAACATTCTACAATATTGCTTTATCACTCAGTGTTCTAGAAGTCTCATTTGGTTTTAAGGTTCTATTTGTTTGTTTGGAAACAGTTCAGTTTGCTGTTACAATTGATATTTTCTACTTTTTCAAGAATAACCCGGATGCTCACAAATATTTTAAAAAATAAAAATAAACATACAAAAGGTTGTCTATTTTATCTTTTAACAGCAGAAACGCTTGAAGTGCATCAGTGAATCACAATCGATATTGAATCTATATTGCTAAATGGAAATGTTATCACTTTTCAACTTTTATTCTTATTGCATAATTTCATGGAAACTCGAGCCAGTTTATTGAGAGTCTCATCAAGTATAGGAATTGGTGTACATATTGATTTGCTGTACATTGCATAAGTTACTGCCGAGAATATAAGATATTTCGTACTGAAATAATGATATGCCATTTCAAAAGTACTGGATGTGACATCCTTGTCTCTTCTTCTTCATTTTAAATTTTTTTTGATCTGAAAGGATTCTATTCGGAATCTTCACATGTAAGCAAATTCATGTCATCGGTTAGTACATTTTAGCAGCTTACAATAGTATGGATATTTGTGGCATCTGCTTATTCTGTTTCTTCTATTTTAATTCTTTTGCAGGTTCATATACTTGTTCATTGCTGTGGGAGTCATTATCTTTGTTGTTTCTTGTTTTGGGTGCATTGGAGCTGCAACACGTAATGGATGCTGTTTAAGTTGTGTATCCTATTTGCCTGCATGGCTTCATTGTTGTTTGATTTATGAATTAGTAATGAAACAATTAGGCCACCAGGGAACTTGTTGGCTTCGGGATAACTACACTGTTCATATAGATAAGATGGTGATCTTAAATATAATACAACATAGTCATGATAACACGTACGGTTTAATCTAAAAAAATTGACAGTGATCTCCATTTGAGTTTTATGTTACTGTATCCTTAATTAATGATCACAGTATTCAGTTTTGGTGCTTCTACTGATTTTGGTGCAAGTAGGATGTGCAGCCTTCATATTCTTTGACAAACATTGGAGAGATGTGAGTGGTTCACCTCACAAACTAAAATTGGAATTTTAGTTCATTTTCTTATTATTATTACTGTTATTCCATAGGAAATTCCTGGGGACAAAACAGGAAACTTCGATAAGATCTATGAACTCCTCGAAGACAAGTGGGAAATCATCAGATGGGTTGCACTAGGAGCTGTAATTTTTGAGGTACGTGAACCCCTTTTTGAAGCTTTTGAATCTTAGACTGGCATGGCAGAGGCCTAATGCTGCTCTAACCTCTGAATGTCTTGCTTAGCAGAAATGTTACATGCACTGTAATTACTAAATTACTGGGATCTTCGTTATTTCTAATTCATGTGAATATATAACTTAATTTCGCATAAATATTGAATATTGTTGTAATTGTTTTATTATAGAATATAATAATTATATAATGATTAATAGAATATATCTATAGAAATTTATTATTTAATTATCAAATAGATTTGTATCAATAATTGATGTGTTAATCCCAATTTGGTCCATTTTTGTTTTTGAATTCAAATGACATTTGAAATTCTTTTTCTTTTCTGATTTTTGGTTAATCTTATTTTAGTCTTTTTAGTAATCCACTCTACTGATTGAATTGTATCTTTTTAGTTATCTACTCTTTACTGATTGACTTGTATACCATTCTTTACTATGTTTGTGTGCATTAAACATTGCACTTCTCAAGAAACAGATTGACCTGTTTCTTGCTGCTGAAACTCTGTCATCAAGAATATGTACTTCTACGCCCCTCATCAAATTTTAGGGTTTTTTTTTTTTTCTCCTTTTTGCATCTCTCTTGAATTCTGGTTTAGTGCATTTTTTCCGCCATAGAGTTTCAAACATATGCGGTTGGTAGGTTCTTTAATTCGCAATTTGTGATTTGCAATATAAAATTCATTTTACCCTTTCTCACATCAATAATTTACAAGAATTTGGTTTTTGTTGATCGCCAAAGAAGACTTCATATATAATACTATCAATGGATTGCCCCAACCTTTTCTCCCTAACATATCGATTTGCTTTACTTGCTGTTTTTTCCCTTTATTTGATTTAACAACTTTTGTGCTTTTGGCCTTGAGCCCTACTCTTATCTACTCAAAACTCCCTTCTTTAGATACCAATTTGGCCATCTGGATTTATTTGCTTTACACTGATTGTACGTTCTTTTTTCTCTCATTTGATAATCATTAGAATTTCCAACTTGTAATTTGTGTTTCATTTGTATTTGCAGGCTCATGCCTTTTTCTTCAAGTTGGCTCTATGTGGTGCGCTGCGCAGCAAACAGTCCTGCAGACTATGA

mRNA sequence

TTGTTTTTAGGGATCGAAAGCGTATAGAGGATGGCCTGCAGAGGGTGCTTTGAGTGCCTGTTGAAGCTTCTGAACTTCTTCTTATCCCTTCTGGGTCTTGCCATGGTGGGGTATGGGATTTACTTGTTGGTTGAGTACTTGCAATCTCCTAGTGATATCCCAAGACCTCCGTTGAGTGGTGATCACGATCTGGTCCAGCTTGGTCGACCAATGCTAATGGCCGTGTCTCTGTCTTCTAACATCTTTGATCACCTTCCAAAAGCCTGGTTCATATACTTGTTCATTGCTGTGGGAGTCATTATCTTTGTTGTTTCTTGTTTTGGGTGCATTGGAGCTGCAACACTTTTGGTGCTTCTACTGATTTTGGTGCAAGTAGGATGTGCAGCCTTCATATTCTTTGACAAACATTGGAGAGATGAAATTCCTGGGGACAAAACAGGAAACTTCGATAAGATCTATGAACTCCTCGAAGACAAGTGGGAAATCATCAGATGGGTTGCACTAGGAGCTGTAATTTTTGAGGCTCATGCCTTTTTCTTCAAGTTGGCTCTATGTGGTGCGCTGCGCAGCAAACAGTCCTGCAGACTATGA

Coding sequence (CDS)

ATGGCCTGCAGAGGGTGCTTTGAGTGCCTGTTGAAGCTTCTGAACTTCTTCTTATCCCTTCTGGGTCTTGCCATGGTGGGGTATGGGATTTACTTGTTGGTTGAGTACTTGCAATCTCCTAGTGATATCCCAAGACCTCCGTTGAGTGGTGATCACGATCTGGTCCAGCTTGGTCGACCAATGCTAATGGCCGTGTCTCTGTCTTCTAACATCTTTGATCACCTTCCAAAAGCCTGGTTCATATACTTGTTCATTGCTGTGGGAGTCATTATCTTTGTTGTTTCTTGTTTTGGGTGCATTGGAGCTGCAACACTTTTGGTGCTTCTACTGATTTTGGTGCAAGTAGGATGTGCAGCCTTCATATTCTTTGACAAACATTGGAGAGATGAAATTCCTGGGGACAAAACAGGAAACTTCGATAAGATCTATGAACTCCTCGAAGACAAGTGGGAAATCATCAGATGGGTTGCACTAGGAGCTGTAATTTTTGAGGCTCATGCCTTTTTCTTCAAGTTGGCTCTATGTGGTGCGCTGCGCAGCAAACAGTCCTGCAGACTATGA

Protein sequence

MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRPMLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATLLVLLLILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRSKQSCRL
Homology
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BLAST of Carg27408 vs. NCBI nr
Match: KAG7011516.1 (Tobamovirus multiplication protein 2A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 2.8e-99
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATLLVLLLILVQVGCAAF 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATLLVLLLILVQVGCAAF
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATLLVLLLILVQVGCAAF 120

Query: 121 IFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRS 180
           IFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRS
Sbjct: 121 IFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRS 180

Query: 181 KQSCRL 187
           KQSCRL
Sbjct: 181 KQSCRL 186

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BLAST of Carg27408 vs. NCBI nr
Match: XP_023553897.1 (tobamovirus multiplication protein 2A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 322.8 bits (826), Expect = 2.0e-84
Identity = 167/187 (89.30%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 178
           ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

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BLAST of Carg27408 vs. NCBI nr
Match: XP_022952814.1 (tobamovirus multiplication protein 2A [Cucurbita moschata])

HSP 1 Score: 320.5 bits (820), Expect = 9.8e-84
Identity = 166/187 (88.77%), Postives = 168/187 (89.84%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 178
           ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

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BLAST of Carg27408 vs. NCBI nr
Match: XP_022971990.1 (tobamovirus multiplication protein 2A-like [Cucurbita maxima] >XP_022971992.1 tobamovirus multiplication protein 2A-like [Cucurbita maxima])

HSP 1 Score: 320.5 bits (820), Expect = 9.8e-84
Identity = 165/187 (88.24%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLV+YLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVDYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 178
           ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEII+WVALGAVIFEA  F  
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIKWVALGAVIFEALLFLL 180

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BLAST of Carg27408 vs. NCBI nr
Match: XP_022931040.1 (tobamovirus multiplication protein 2A-like [Cucurbita moschata] >KAG7036173.1 Tobamovirus multiplication protein 2A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.5 bits (802), Expect = 1.2e-81
Identity = 161/189 (85.19%), Postives = 169/189 (89.42%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+PRPPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFD+LP+AWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 180
           ILVQ+GCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

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BLAST of Carg27408 vs. ExPASy Swiss-Prot
Match: Q9C5W7 (Tobamovirus multiplication protein 2A OS=Arabidopsis thaliana OX=3702 GN=TOM2A PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.2e-56
Identity = 111/188 (59.04%), Postives = 137/188 (72.87%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPRPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAA----------TLLVLL 120
           P+LMAVSLSSNIFD+LPKAWFIYLFI +GV +FV+SC GC+G            +LL++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFF 178
           LILV++G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I+RWVALGAV+FEA  F 
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

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BLAST of Carg27408 vs. ExPASy Swiss-Prot
Match: Q93XY5 (Tetraspanin-18 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH2 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 57/199 (28.64%), Postives = 95/199 (47.74%), Query Frame = 0 

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPL---SGDHDLVQLGR 62
           C   F   LK+LNF  + +G++++ Y I++L EY +     P P     SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNIFDH--------LPKAWFIYLFIAVGVIIFVVSCFGCIGAA-- 122
           P+      + ++ L SN  DH        LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 --------TLLVLLLILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRW 175
                   ++L  LLIL++    A+I  D+HW  ++P D TG    +   +E+  +I +W
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

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BLAST of Carg27408 vs. ExPASy Swiss-Prot
Match: Q9SVU4 (Tetraspanin-20 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH1 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-13
Identity = 56/219 (25.57%), Postives = 101/219 (46.12%), Query Frame = 0 

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS-PSDIP--RPPLSGDHD------ 62
           C   F  +LK+LNF  + +G++++ Y I++L +Y    P D P  +PP +   D      
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  --LVQLGR---------PMLMAVSLSSNIFD--------HLPKAWFIYLFIAVGVIIFVV 122
             ++++             +  + L S   D         LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGAA----------TLLVLLLILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYE 182
           +  G I A           ++L  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 LLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRSKQS 184
            +E+  +I +WV +  V  +  +    L L   +  +QS
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQS 224

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BLAST of Carg27408 vs. ExPASy Swiss-Prot
Match: Q940P5 (Tetraspanin-19 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.5e-13
Identity = 47/181 (25.97%), Postives = 87/181 (48.07%), Query Frame = 0 

Query: 4   RGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRPMLM 63
           R C + +LKL+N  + ++G+AM+ Y ++L+ ++ +   ++P      DH           
Sbjct: 6   RSCLQSMLKLVNSLIGMVGIAMILYAVWLIRQWQEQMGNLPF--ADSDH----------- 65

Query: 64  AVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATL----------LVLLLILV 123
                       P  WFIY F+ +G I+ VV+C G I A T+           ++LL +V
Sbjct: 66  ------------PVPWFIYSFLGLGAILCVVTCAGHIAAETVNGCCLYLYMGFIVLLTMV 125

Query: 124 QVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFFKLA 175
           + G  A IF ++ W+ + P D +G F +  + +E  ++I +W+ L  V  +  +    + 
Sbjct: 126 EGGVVADIFLNRDWKKDFPEDPSGAFHQFSKFIESNFKICKWIGLSIVCVQGLSVLIAML 161

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BLAST of Carg27408 vs. ExPASy TrEMBL
Match: A0A6J1I3F8 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111470643 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.7e-84
Identity = 165/187 (88.24%), Postives = 169/187 (90.37%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLV+YLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVDYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 178
           ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEII+WVALGAVIFEA  F  
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIKWVALGAVIFEALLFLL 180

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BLAST of Carg27408 vs. ExPASy TrEMBL
Match: A0A6J1GMT3 (tobamovirus multiplication protein 2A OS=Cucurbita moschata OX=3662 GN=LOC111455402 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.7e-84
Identity = 166/187 (88.77%), Postives = 168/187 (89.84%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 178
           ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

[Close]
BLAST of Carg27408 vs. ExPASy TrEMBL
Match: A0A6J1EYE4 (tobamovirus multiplication protein 2A-like OS=Cucurbita moschata OX=3662 GN=LOC111437351 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.8e-82
Identity = 161/189 (85.19%), Postives = 169/189 (89.42%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+PRPPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFD+LP+AWFIYLFIAVGVIIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 180
           ILVQ+GCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

[Close]
BLAST of Carg27408 vs. ExPASy TrEMBL
Match: A0A6J1K6M6 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111491627 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.7e-81
Identity = 160/189 (84.66%), Postives = 168/189 (88.89%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+PRPPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFD+LP+AWFIYLFIAVG IIFVVSCFGCIGAAT          +LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGAIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 180
           ILVQ+GCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEA  F  
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

[Close]
BLAST of Carg27408 vs. ExPASy TrEMBL
Match: A0A5A7T8L3 (Tobamovirus multiplication protein 2A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00630 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.6e-78
Identity = 154/189 (81.48%), Postives = 165/189 (87.30%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDLVQLGRP
Sbjct: 90  MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPGPSLSGDHDLVQLGRP 149

Query: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAAT----------LLVLLL 120
           MLMAVSLSSNIFD+LPKAWFIYLFIA GVI+FVVSCFGCIGAAT          +L+LLL
Sbjct: 150 MLMAVSLSSNIFDNLPKAWFIYLFIATGVIVFVVSCFGCIGAATRSGCCLSCYSILLLLL 209

Query: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFFF 180
           ILVQ+GCAAFIFFDK+WRDEIPGDKTGNFDKIYELLE+KW+IIRWVALG +IFEA  F  
Sbjct: 210 ILVQLGCAAFIFFDKNWRDEIPGDKTGNFDKIYELLENKWKIIRWVALGTIIFEALLFLL 269

[Close]
BLAST of Carg27408 vs. TAIR 10
Match: AT1G32400.1 (tobamovirus multiplication 2A )

HSP 1 Score: 218.8 bits (556), Expect = 3.7e-57
Identity = 111/188 (59.04%), Postives = 137/188 (72.87%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPRPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAA----------TLLVLL 120
           P+LMAVSLSSNIFD+LPKAWFIYLFI +GV +FV+SC GC+G            +LL++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFF 178
           LILV++G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I+RWVALGAV+FEA  F 
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

[Close]
BLAST of Carg27408 vs. TAIR 10
Match: AT1G32400.2 (tobamovirus multiplication 2A )

HSP 1 Score: 218.8 bits (556), Expect = 3.7e-57
Identity = 111/188 (59.04%), Postives = 137/188 (72.87%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPRPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAA----------TLLVLL 120
           P+LMAVSLSSNIFD+LPKAWFIYLFI +GV +FV+SC GC+G            +LL++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFF 178
           LILV++G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I+RWVALGAV+FEA  F 
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

[Close]
BLAST of Carg27408 vs. TAIR 10
Match: AT1G32400.3 (tobamovirus multiplication 2A )

HSP 1 Score: 218.8 bits (556), Expect = 3.7e-57
Identity = 111/188 (59.04%), Postives = 137/188 (72.87%), Query Frame = 0 

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPRPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAA----------TLLVLL 120
           P+LMAVSLSSNIFD+LPKAWFIYLFI +GV +FV+SC GC+G            +LL++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEAHAFF 178
           LILV++G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I+RWVALGAV+FEA  F 
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

[Close]
BLAST of Carg27408 vs. TAIR 10
Match: AT2G20230.1 (Tetraspanin family protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-16
Identity = 57/199 (28.64%), Postives = 95/199 (47.74%), Query Frame = 0 

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPL---SGDHDLVQLGR 62
           C   F   LK+LNF  + +G++++ Y I++L EY +     P P     SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNIFDH--------LPKAWFIYLFIAVGVIIFVVSCFGCIGAA-- 122
           P+      + ++ L SN  DH        LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 --------TLLVLLLILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRW 175
                   ++L  LLIL++    A+I  D+HW  ++P D TG    +   +E+  +I +W
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

[Close]
BLAST of Carg27408 vs. TAIR 10
Match: AT4G28770.1 (Tetraspanin family protein )

HSP 1 Score: 78.2 bits (191), Expect = 7.9e-15
Identity = 56/219 (25.57%), Postives = 101/219 (46.12%), Query Frame = 0 

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS-PSDIP--RPPLSGDHD------ 62
           C   F  +LK+LNF  + +G++++ Y I++L +Y    P D P  +PP +   D      
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  --LVQLGR---------PMLMAVSLSSNIFD--------HLPKAWFIYLFIAVGVIIFVV 122
             ++++             +  + L S   D         LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGAA----------TLLVLLLILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYE 182
           +  G I A           ++L  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 LLEDKWEIIRWVALGAVIFEAHAFFFKLALCGALRSKQS 184
            +E+  +I +WV +  V  +  +    L L   +  +QS
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQS 224

The following BLAST results are available for this feature:
BLAST of Carg27408 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7011516.12.8e-99100.00Tobamovirus multiplication protein 2A [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023553897.12.0e-8489.30tobamovirus multiplication protein 2A-like [Cucurbita pepo subsp. pepo][more]
XP_022952814.19.8e-8488.77tobamovirus multiplication protein 2A [Cucurbita moschata][more]
XP_022971990.19.8e-8488.24tobamovirus multiplication protein 2A-like [Cucurbita maxima] >XP_022971992.1 to... [more]
XP_022931040.11.2e-8185.19tobamovirus multiplication protein 2A-like [Cucurbita moschata] >KAG7036173.1 To... [more]
BLAST of Carg27408 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C5W75.2e-5659.04Tobamovirus multiplication protein 2A OS=Arabidopsis thaliana OX=3702 GN=TOM2A P... [more]
Q93XY52.0e-1528.64Tetraspanin-18 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH2 PE=1 SV=1[more]
Q9SVU41.1e-1325.57Tetraspanin-20 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH1 PE=1 SV=1[more]
Q940P55.5e-1325.97Tetraspanin-19 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH3 PE=2 SV=1[more]
BLAST of Carg27408 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I3F84.7e-8488.24tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GMT34.7e-8488.77tobamovirus multiplication protein 2A OS=Cucurbita moschata OX=3662 GN=LOC111455... [more]
A0A6J1EYE45.8e-8285.19tobamovirus multiplication protein 2A-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K6M61.7e-8184.66tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7T8L38.6e-7881.48Tobamovirus multiplication protein 2A OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
BLAST of Carg27408 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G32400.13.7e-5759.04tobamovirus multiplication 2A [more]
AT1G32400.23.7e-5759.04tobamovirus multiplication 2A [more]
AT1G32400.33.7e-5759.04tobamovirus multiplication 2A [more]
AT2G20230.11.4e-1628.64Tetraspanin family protein [more]
AT4G28770.17.9e-1525.57Tetraspanin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR19282TETRASPANINcoord: 2..173
NoneNo IPR availablePANTHERPTHR19282:SF417TOBAMOVIRUS MULTIPLICATION PROTEIN 2Acoord: 2..173

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27408-RACarg27408-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane