Carg27402 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27402
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGlycine cleavage system H protein
LocationCarg_Chr19: 5119883 .. 5120723 (+)
RNA-Seq ExpressionCarg27402
SyntenyCarg27402
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGTATGAAGTTGGGTTTTGTTTTTTTTGCTGTTGGATCTCGTTTCCGTGCTGTAGTGTTGTTTGGTTGTTGAGTATGTTTAATTTGTTATCTGGGTATCATAAAAGAGGAGAGTTTTTTGTTCTTAGTTGAATTTAAAGCTGTTGGTTGGGTTTGCAGTTTTGGATGGACTGAAGTATGCTTCTTCTCATGAGTGGGTGAAGCATGAGGGTGAGGTTGCTACTGTTGGCATCACTGACCATGCTCAGGTGCTTTCTCTTTTTTCCATCACTGAACGTCCATTAATGACGATCATCCTTTAAATTTTGTCTAATAGGTTTACGTGGTTAACTCCACTATTTTAATGCTAACATGATATGTTTTTACTATGGGGTTGCATTGGAAATGAACCCACTGACATATAATTGGAATATACACGATCTGTGTAAGATTCCACGTCGGATGGGGAGGAGAACGAAACATTCTTGATAAGGGTGTGGGGCGGTCACGAGGCGCGAGCAAACAGATTCAATCTTTTATTTTAATTACTTTTACATTAATCCATTCCAGAAATTATGCATTTATCTTGATATTCAAACATGGGTTGGAAGCGTTTTGTTTGATGCATTTTCACAGGACCATCTAGGAGAAGTGGTGTTTGTGGATCTGCCAGAATCAGGTGGTTCAGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAAAGTGTGAAAGCAACAAGTGATATCAACTCCCCAATCTCTGGTGAAATTGTTGAAGTTAACCCACAGCTTAAGGATTCTCCTGGTTTGGTAATTCCAATCCCAAACTCTTTTTGCCAATTCTTTGGACTTTGCATTTGA

mRNA sequence

ATGCCTGACCATCTAGGAGAAGTGGTGTTTGTGGATCTGCCAGAATCAGGTGGTTCAGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAAAGTGTGAAAGCAACAAGTGATATCAACTCCCCAATCTCTGGTGAAATTGTTGAAGTTAACCCACAGCTTAAGGATTCTCCTGGTTTGGTAATTCCAATCCCAAACTCTTTTTGCCAATTCTTTGGACTTTGCATTTGA

Coding sequence (CDS)

ATGCCTGACCATCTAGGAGAAGTGGTGTTTGTGGATCTGCCAGAATCAGGTGGTTCAGTTTCTCAAGGTAAGAGCTTTGGAGCAGTGGAAAGTGTGAAAGCAACAAGTGATATCAACTCCCCAATCTCTGGTGAAATTGTTGAAGTTAACCCACAGCTTAAGGATTCTCCTGGTTTGGTAATTCCAATCCCAAACTCTTTTTGCCAATTCTTTGGACTTTGCATTTGA

Protein sequence

MPDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIPIPNSFCQFFGLCI
Homology
[Close]
BLAST of Carg27402 vs. NCBI nr
Match: KAG7011522.1 (Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 154.1 bits (388), Expect = 4.9e-34
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0 

Query: 1  MPDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLV 60
          MPDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLV
Sbjct: 1  MPDHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLV 60

Query: 61 IPIPNSFCQFFGLCI 76
          IPIPNSFCQFFGLCI
Sbjct: 61 IPIPNSFCQFFGLCI 75

[Close]
BLAST of Carg27402 vs. NCBI nr
Match: KAG6571837.1 (Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 115.2 bits (287), Expect = 2.5e-22
Identity = 58/62 (93.55%), Postives = 59/62 (95.16%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL+  
Sbjct: 63  DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLINS 122

Query: 63  IP 65
            P
Sbjct: 123 SP 124

[Close]
BLAST of Carg27402 vs. NCBI nr
Match: XP_022952526.1 (glycine cleavage system H protein, mitochondrial-like [Cucurbita moschata] >XP_022972003.1 glycine cleavage system H protein, mitochondrial-like [Cucurbita maxima] >XP_023553680.1 glycine cleavage system H protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 115.2 bits (287), Expect = 2.5e-22
Identity = 58/62 (93.55%), Postives = 59/62 (95.16%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL+  
Sbjct: 67  DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. NCBI nr
Match: XP_038876916.1 (glycine cleavage system H protein, mitochondrial [Benincasa hispida])

HSP 1 Score: 113.6 bits (283), Expect = 7.3e-22
Identity = 57/62 (91.94%), Postives = 59/62 (95.16%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLK+SPGL+  
Sbjct: 67  DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKESPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. NCBI nr
Match: XP_004148493.1 (glycine cleavage system H protein 3, mitochondrial [Cucumis sativus] >KGN60412.1 hypothetical protein Csa_002081 [Cucumis sativus])

HSP 1 Score: 111.3 bits (277), Expect = 3.6e-21
Identity = 56/62 (90.32%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESG SVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLK+SPGL+  
Sbjct: 67  DHLGEVVFVDLPESGSSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKESPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match: Q39732 (Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala OX=35877 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.7e-19
Identity = 46/62 (74.19%), Postives = 54/62 (87.10%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPE+GGSV++   FGAVESVKATSD+NSPISGEIVEVN +L ++PGL+  
Sbjct: 64  DHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSETPGLINS 123

Query: 63  IP 65
            P
Sbjct: 124 SP 125

[Close]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match: P49359 (Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei OX=4226 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.7e-19
Identity = 46/62 (74.19%), Postives = 54/62 (87.10%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPE+GGSV++   FGAVESVKATSD+NSPISGEIVEVN +L ++PGL+  
Sbjct: 64  DHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSETPGLINS 123

Query: 63  IP 65
            P
Sbjct: 124 SP 125

[Close]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match: P49360 (Glycine cleavage system H protein, mitochondrial (Fragment) OS=Flaveria pubescens OX=35880 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.7e-19
Identity = 46/62 (74.19%), Postives = 54/62 (87.10%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPE+GGSV++   FGAVESVKATSD+NSPISGEIVEVN +L ++PGL+  
Sbjct: 64  DHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSETPGLINS 123

Query: 63  IP 65
            P
Sbjct: 124 SP 125

[Close]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match: O82179 (Glycine cleavage system H protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GDH2 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.6e-19
Identity = 46/62 (74.19%), Postives = 55/62 (88.71%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLG+VV+V+LP+ G SVSQGKSFGAVESVKATSDINSP+SG++VEVN +L +SPGLV  
Sbjct: 58  DHLGDVVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTESPGLVNS 117

Query: 63  IP 65
            P
Sbjct: 118 SP 119

[Close]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match: P46485 (Glycine cleavage system H protein, mitochondrial OS=Flaveria trinervia OX=4227 GN=GDCSH PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.1e-18
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFV+LPE GGSV++   FGAVESVKATSD+NSPISGEIVEVN +L  +PGL+  
Sbjct: 66  DHLGEVVFVELPEVGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLTKTPGLINK 125

Query: 63  IP 65
            P
Sbjct: 126 SP 127

[Close]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match: A0A6J1I8M1 (Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111470652 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-22
Identity = 58/62 (93.55%), Postives = 59/62 (95.16%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL+  
Sbjct: 67  DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match: A0A6J1GKG7 (Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111455190 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-22
Identity = 58/62 (93.55%), Postives = 59/62 (95.16%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL+  
Sbjct: 67  DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match: A0A0A0LI67 (Glycine cleavage system H protein OS=Cucumis sativus OX=3659 GN=Csa_3G903520 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.8e-21
Identity = 56/62 (90.32%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESG SVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLK+SPGL+  
Sbjct: 67  DHLGEVVFVDLPESGSSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKESPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match: A0A6J1JYK8 (Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111490918 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.3e-21
Identity = 56/62 (90.32%), Postives = 57/62 (91.94%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESG  VSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGL+  
Sbjct: 67  DHLGEVVFVDLPESGAPVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match: A0A1S3CQ17 (Glycine cleavage system H protein OS=Cucumis melo OX=3656 GN=LOC103503516 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.9e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFVDLPESG SVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLK++PGL+  
Sbjct: 67  DHLGEVVFVDLPESGSSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKETPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

[Close]
BLAST of Carg27402 vs. TAIR 10
Match: AT2G35120.1 (Single hybrid motif superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 3.3e-20
Identity = 46/62 (74.19%), Postives = 55/62 (88.71%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLG+VV+V+LP+ G SVSQGKSFGAVESVKATSDINSP+SG++VEVN +L +SPGLV  
Sbjct: 58  DHLGDVVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTESPGLVNS 117

Query: 63  IP 65
            P
Sbjct: 118 SP 119

[Close]
BLAST of Carg27402 vs. TAIR 10
Match: AT1G32470.1 (Single hybrid motif superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-18
Identity = 44/62 (70.97%), Postives = 53/62 (85.48%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFV+LPE+  SVS+ KSFGAVESVKATS+I SPISGE++EVN +L +SPGL+  
Sbjct: 68  DHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLTESPGLINS 127

Query: 63  IP 65
            P
Sbjct: 128 SP 129

[Close]
BLAST of Carg27402 vs. TAIR 10
Match: AT2G35370.1 (glycine decarboxylase complex H )

HSP 1 Score: 88.2 bits (217), Expect = 3.1e-18
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 0 

Query: 3   DHLGEVVFVDLPESGGSVSQGKSFGAVESVKATSDINSPISGEIVEVNPQLKDSPGLVIP 62
           DHLGEVVFV+LPE   SVS+ KSFGAVESVKATS+I SPISGEI+EVN +L +SPGL+  
Sbjct: 67  DHLGEVVFVELPEDNTSVSKEKSFGAVESVKATSEILSPISGEIIEVNKKLTESPGLINS 126

Query: 63  IP 65
            P
Sbjct: 127 SP 128

The following BLAST results are available for this feature:
BLAST of Carg27402 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7011522.14.9e-34100.00Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperm... [more]
KAG6571837.12.5e-2293.55Glycine cleavage system H protein, mitochondrial, partial [Cucurbita argyrosperm... [more]
XP_022952526.12.5e-2293.55glycine cleavage system H protein, mitochondrial-like [Cucurbita moschata] >XP_0... [more]
XP_038876916.17.3e-2291.94glycine cleavage system H protein, mitochondrial [Benincasa hispida][more]
XP_004148493.13.6e-2190.32glycine cleavage system H protein 3, mitochondrial [Cucumis sativus] >KGN60412.1... [more]
BLAST of Carg27402 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q397322.7e-1974.19Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala OX=35877 GN... [more]
P493592.7e-1974.19Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei OX=4226 GN... [more]
P493602.7e-1974.19Glycine cleavage system H protein, mitochondrial (Fragment) OS=Flaveria pubescen... [more]
O821794.6e-1974.19Glycine cleavage system H protein 2, mitochondrial OS=Arabidopsis thaliana OX=37... [more]
P464855.1e-1872.58Glycine cleavage system H protein, mitochondrial OS=Flaveria trinervia OX=4227 G... [more]
BLAST of Carg27402 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I8M11.2e-2293.55Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111470652 PE... [more]
A0A6J1GKG71.2e-2293.55Glycine cleavage system H protein OS=Cucurbita moschata OX=3662 GN=LOC111455190 ... [more]
A0A0A0LI671.8e-2190.32Glycine cleavage system H protein OS=Cucumis sativus OX=3659 GN=Csa_3G903520 PE=... [more]
A0A6J1JYK82.3e-2190.32Glycine cleavage system H protein OS=Cucurbita maxima OX=3661 GN=LOC111490918 PE... [more]
A0A1S3CQ173.9e-2188.71Glycine cleavage system H protein OS=Cucumis melo OX=3656 GN=LOC103503516 PE=3 S... [more]
BLAST of Carg27402 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G35120.13.3e-2074.19Single hybrid motif superfamily protein [more]
AT1G32470.11.8e-1870.97Single hybrid motif superfamily protein [more]
AT2G35370.13.1e-1872.58glycine decarboxylase complex H [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033753Glycine cleavage system H-protein/SimiatePFAMPF01597GCV_Hcoord: 3..60
e-value: 4.0E-21
score: 75.2
IPR033753Glycine cleavage system H-protein/SimiateCDDcd06848GCS_Hcoord: 3..60
e-value: 3.08997E-27
score: 92.5966
NoneNo IPR availableGENE3D2.40.50.100coord: 1..66
e-value: 2.4E-22
score: 80.9
NoneNo IPR availablePANTHERPTHR11715:SF33GLYCINE CLEAVAGE SYSTEM H PROTEINcoord: 3..63
IPR002930Glycine cleavage system H-proteinPANTHERPTHR11715GLYCINE CLEAVAGE SYSTEM H PROTEINcoord: 3..63
IPR0030162-oxo acid dehydrogenase, lipoyl-binding sitePROSITEPS00189LIPOYLcoord: 17..46
IPR000089Biotin/lipoyl attachmentPROSITEPS50968BIOTINYL_LIPOYLcoord: 1..75
score: 18.363203
IPR011053Single hybrid motifSUPERFAMILY51230Single hybrid motifcoord: 3..60

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27402-RACarg27402-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019464 glycine decarboxylation via glycine cleavage system
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005960 glycine cleavage complex
cellular_component GO:0005739 mitochondrion