Carg27401 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27401
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	beta-amyrin 11-oxidase-like
Location	Carg_Chr19: 5126420 .. 5131962 (-)
RNA-Seq Expression	Carg27401
Synteny	Carg27401

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAAGAAAAAAAAGAAAAAAAAAAGAAAAAATGGAGGTTTGGGTATGGCTGGTCGGAGCTTTGGTCGGTGCATACATTTGTGTGTTTGGGATTCTCAGAAACCTTAATCAATGGCGGTTTGTCACCCGACACGGCGGCCGTTACAATCTCCCTCCCGGCGATATGGGCTGGCCGCTCGTCGGAACTCTTCTCCCCTTTCTTCAAGCTTTCCGATCGGGTCGACCCGATTCCTTCATTCATCACTTTGCCTCAAAGTAATTCTCTCTCTCTCCCTCTCGCCGGAGTCTTTGGATCCCAAATTCTTCTTAATTTCTTTCAATTTTGTGTTTTTTTTTTTTTTTAATTTTCGATGTTTTTTATTTATTTATTTAAAATTTTTAATTTAAATTTTTTTTTATTAGGGTCAACTATGATGAGCAAAATTTTCAATTTAAACAACTTTTTATTGTATAATGAACTTATCCCAACCCAAAATTTTATAAGTTCGGGTTGATTTGGATCATTTGATTCATTTATTCAATTTTTTTTTTAAATGTAAAATATTTATTTATTTATTTTTAAATATGTAATTAAGATGGAGTCAATCGTATTAATTAAAATAATTTTTTACAGTTAATAGATGAATAAATAATAAAATAAATATATAATAATAAAAATGTCAGATGAATCAATAAAATATTCTTTTTTTTTTTAATTTTTTTTTTAATTTAATCTCGGTACGAATTGAGTTTGGTTGATTGAGTTGGAATGTGTGAACGAACTAAATTCATAAAATTTTCATTTTTTTTATAATTTGACTAATTTCTGGGTTTGGTTGATTGAGTTTGAGTATATAAACAAATTAAATTCATTTTTTTTTTTAATTTAACCCAATTTTTGGGTTTGGTGGATTGAGTTTGAATGTAGGAACCAATTAAATTCATAAAATTTTCATTTTTTTTTAATTTAACTTAATTTTTAGGTTTGGTTGATTGAGTTTAAATGCACGAACCAAATTAAATTCATAAAATTTTTATTTTTTAATTTTAATTTAATTTAATTAATTGAGTTAGGTGGATTGAGTTTTTTTTAAGGTCAATAAAATTTTCTATATTGTGGGTTATTATTATTATTATTTTTTTTATCACTTTTTGATCCAATATTAAATTAAAAAATACAGTAAAAAAGGAATTAAAATTTAATTTATTTTTAAAAAATGGATGAAATTTCAGGTATGGGAAAATAGGGATGTACAAGACGTACTTATTCGGGAGCCCAAGCGTGATCGTATGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGCCTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTCTCAACACCGTCTCCAGGCCCGAGCACCGCCGTATACGCCGCCTCATCGCCTCCCTAATCAGCGGCAACGATGCGCTGTCGATCTACATAGGTCACGTGGAGGGGATTGTGATCACGTGCCTAGAGGAGTGGGCCACCATGAAGAAGCCGGTCGAGTTCTTGTCGGAGATGAAAACGATGGCGTTTAAGATTTTGTTACACATATTCATCGGAGCTAATACGGCGGCGTTTATAGATAAGATGGAGAAGCTCTACACTGATTTCCACTTGGGGTTCATGTCCTCCGCCGTCGATATTCCCGGAACTACCTTCAGCCGAGCTCTCAAGGTAACTTCGTAAAAGATCGAACTTTCAACTTTTTTTTTTTTAAAAAAAAAAAAAAAAAACTAAAAGATCAAAACACTAACTATATGTGAGTCGGGTCCAGTCCAGAGATTCGAAACTCTAACCATCGATTCTTGTGAGTCGGGTCCAGTCCAAAGATTCGAAACTCTAACCATCGATTCTAACCTTCTAATCGATGATATATGCACGATGGTAATAGAGCTATGCTAATGTGAGATCTCACATCGGTTGGGGAGGAGAACGAAACACTCTTTATAAGGGTGTGGAAACCTCTCCCTACTAGACGCGTTTTAAAACCGTGAGCTGTGGTATTGGACTGTTACAAATGGTATCAAAGCCATACACGGGATGATGTGCAATCAATCGGGGAAAGGAATGAGTGTCAGCGCAAGGACGCTGGGCTCTAAAGGGGTGTGGATTATGAGATCCCACGTTAGTTGGGGACGAGAACGAAACCTTTATAAAGGTGTAGAAATCTCTTTCTAGCAGACGTGTTTTAAAACCGTAAGGCTGACGGCGATACGTAATGTGATATCTCACATTGGTTGGGGAGGAGAACGAAACACCCGAAACACCCTTTATAGTGTAGAAACATTTTTCTAGCAGATGCGTTTTGAAACGGAAGCCCGGAAAAGAAATATAATTTTATGTTAAAAAATTGATTTTGGTTTTATATACTATATATATATTTTTTCATTTATTTGAGGAACATTCATAGTCTTAACAAGTCAACGAGGTGGAATTAAGGGTTTAAAGATTAAAGGCTTAAAATTAATTTCCACGTAGACTTATTTATTTTATTTTGATTTAACACGAAATTTTATAGAGTATTTAATTATGTGTAAATTTGTATTATTTTATTTAATATTAAATTTATAAATGAGTATGAAAACGTAACGCATCTTAAACATGAAAATTTAATTTAATAACGAAAAGCCGGAAAATCTGACTCATGACATTCAAATGAATTTGTATTTATTATAAAGTTAATAAAATAATAATAACTAATAATAATAATTTATTTTTAATTTTGAGTTTTTTCAGATAAATTAATAAAAATATTATTAAATTTTTATTACTTTAAAACATATTCCAAAACTTTGAAAAAAGTCTTAAAAAAATTTACTTTAATTTTTAAAATTATTTCAAAGATAAGTATAATAGTTTCTATTAAGATTTTATTTTTTAAAAAGTTTTAGGTATTACTACCAAATTTAGGAATATTTTTATTAATTTAATTTTTTTTAACGGTTAATTTTGGTTTTTGTATTTTACCTAACATGCCAACTTATGATATACTCATGTTATGAAAGTCCAACATTTGCTAATTTAGAGAATGATCATGAGTTTATAATCAAAGAATACTGTCTCTATTGATATGAGGTTTCTTGGAGAAGCCGCATGACTGGACAATATCATACTATTGTGGAGAGTCGTGTTCATCTAACATGGTATCAGAGTCATGCCCTAAACTTAGCCATGTCAATAGAGAATCTTCAAATGTCGGGATGAAAGTCCAATATCGACTAGTTTATAATCCGGGAATAATAAAATTCTTATGGGTTTTTTTTGTAATGTAAATTTCAGGCAAGAAACGAGTTGATAAGGATCTTTGAAAGTGTTCTAAAGGAGAAGAGGGAGAATTTGAAGAACAAAGTGGAAGAAAACAGAAAAAAAGACATGACTGATTTGTTGTTGGAAGTTAAAGATGAAGAAGGCCATGGCTTGGATGATGGCTGCATTATTGATTTATTGATCGGTTTCTTCTTCGCTGGCCACGAAACCTCGGCTCATAGTATCATGAGGGCTATCATGTTTCTCTCGGAAAATCCCGAAACCCTTCGTAAAGCTAAGGTTCGTTTCTTCTTCCTACGTTTTCAACCCTCGTTTAATAACCACAAAAAGATTTGATTTTTACGAGCGAACCAGTATTCTCACTTGATGTCTGGTTCTTTATGGTTTTTGTTAAGGGTGTTTAAGAAACTCGGTGACTCGAAAAACTCGATCCAACCTCGATCCAACATAATCTAACCCATATGAGTTAAATTGGGTTATTAACTTATACTGGGTCGAGTCGGGTTTAAATAAATGAAAATATTATAAAAATAAATAAATATATGCTTTAGGCATAAACGTTTTATTTTTATTCAAAATAAAGTTTCATATAAATGACTCGAATGACAGGAACCAATTCAATCCAAAAAATTTATGGTTAGGTTGGGTCATGTCAGATTAAGTTCATTATTTAAAGATCGACTTGCTGTGCAGGCTGAGCAAGATCAAATTGTGAACGCAAGGCCAGCTAATGAACGACAAAGAGGCTTGACCATGAAGGAAATCAAACAAATGGAGTATCTTTCTAAGGTTCATTTTTGTTTTGTTTAAATATTATTATTTTTCGAGATTTATTCGGAACACACTATAATAACATGTTTTTTTTTTGTGGCTTAATTAGGTGATTGATGAGACATTGAGGAAAACGAGCTTGGCCTTCACACTATCGAGAGAGACGAGAAGCGACGTTAACTTAAATGGTTCGTGTAGGGTTACATAATTTGTATTTCATTTCATGGATTAAAATAACTCTTTTTTATGCGTTTTGATTAACCATGGTGATTATTTTGTAGGCTACACCATTCCGAAAGGATGGAAAATCCTTGTTTGGACGAGAGCAGTTCATATGGATCCTGAAATCTACGAAAACCCCGAAAAATTCGACCCCTCTAGATGGGATGTAAGTAGTAAACAAAAAATTTATACTACTCTCCTAATACATTTCATATTCCTTTCATTCACCAAACTTGGTTCTTATTACAAAAAGGTAGAGTTAGTACTATTTTCATTCGTTATCTGCCGGTTCAGGAGGAGAATGAAGCATTGTTTATAAGGGTGTGAAAAACCTCTTCCTAACAGATGAGTTTTTAAAAACCTTGAGGGAAAACTCGAAAGTGAAAACCTAAAAAGGATAATATGTACTAGCGGTGGACTTAAGCTGTTACAAATGGTATCAGAGTTGGGAGGAGAGAGAAGTATTCTTTGTAAGGGTGTGGAAACCTCTCCTTAGAAAATGCATTTTAAAAACCTTGACGGGAAGCCTTAAAGGGAAAGTATAAAGAAGACAATATCTACTAGCGGTGGGCTTGAACCGTTACAAATGGTATTAGAGCTAGACACCGGGCAATGTGCCAGCAAGGAGGCTGAGCCCCGAAGGGGGTGGACACGAGGAGGTGTGCCAGCAATGACACTGGGCCCAAATGGGGGTGGATTGTGAGATCCCACGTCTGTTGGAAGGAGAACGAAACAATAGTTACAAGAGTGTGAAGACCTCTCCCTAACATATGCATTTTAAAAAATTTGAGGGAAAACCCGAAAGAGGACAACAATATCTTTCTTCAAAACAAGATATCGAGTCGGGTTTTACATTCGATTCGATGAATTAACCGTCTTATATTTTTGTTTTCGGTTTCAGAATCCAAAACGAAGAGCTGGATCATTCATTCCTTTCGGAGCAGGAATGAGGCTATGCCCTGGAATTGATCTTTCCAAGCTTGAAATTGCCATTTTTCTTCACTATTTCATCCATGGTGGCTTCAGGTGTTCTTCAAAGCAGTGTTCTTGTTCTTCATTCATGTCAAAATTAAACCAAAAAAATCATTAAAAACTTGACTTTTGTGTTGTTCTTGTGCAGACTTGAGAGAGTAAATCCAAATTGCCCAGAGAATTATCTGCCATTGCCAAGGCCTACAGACAACTGCCTTGCAAGAGTTGTAAGAGACTCATAAACGTTGAAATCAAAAGAGATCATATATGCATATTATATATATATAACG

mRNA sequence

GAAAAAAGAAAAAAAAGAAAAAAAAAAGAAAAAATGGAGGTTTGGGTATGGCTGGTCGGAGCTTTGGTCGGTGCATACATTTGTGTGTTTGGGATTCTCAGAAACCTTAATCAATGGCGGTTTGTCACCCGACACGGCGGCCGTTACAATCTCCCTCCCGGCGATATGGGCTGGCCGCTCGTCGGAACTCTTCTCCCCTTTCTTCAAGCTTTCCGATCGGGTCGACCCGATTCCTTCATTCATCACTTTGCCTCAAAGTATGGGAAAATAGGGATGTACAAGACGTACTTATTCGGGAGCCCAAGCGTGATCGTATGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGCCTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTCTCAACACCGTCTCCAGGCCCGAGCACCGCCGTATACGCCGCCTCATCGCCTCCCTAATCAGCGGCAACGATGCGCTGTCGATCTACATAGGTCACGTGGAGGGGATTGTGATCACGTGCCTAGAGGAGTGGGCCACCATGAAGAAGCCGGTCGAGTTCTTGTCGGAGATGAAAACGATGGCGTTTAAGATTTTGTTACACATATTCATCGGAGCTAATACGGCGGCGTTTATAGATAAGATGGAGAAGCTCTACACTGATTTCCACTTGGGGTTCATGTCCTCCGCCGTCGATATTCCCGGAACTACCTTCAGCCGAGCTCTCAAGGCAAGAAACGAGTTGATAAGGATCTTTGAAAGTGTTCTAAAGGAGAAGAGGGAGAATTTGAAGAACAAAGTGGAAGAAAACAGAAAAAAAGACATGACTGATTTGTTGTTGGAAGTTAAAGATGAAGAAGGCCATGGCTTGGATGATGGCTGCATTATTGATTTATTGATCGGTTTCTTCTTCGCTGGCCACGAAACCTCGGCTCATAGTATCATGAGGGCTATCATGTTTCTCTCGGAAAATCCCGAAACCCTTCGTAAAGCTAAGGCTGAGCAAGATCAAATTGTGAACGCAAGGCCAGCTAATGAACGACAAAGAGGCTTGACCATGAAGGAAATCAAACAAATGGAGTATCTTTCTAAGGTGATTGATGAGACATTGAGGAAAACGAGCTTGGCCTTCACACTATCGAGAGAGACGAGAAGCGACGTTAACTTAAATGGCTACACCATTCCGAAAGGATGGAAAATCCTTGTTTGGACGAGAGCAGTTCATATGGATCCTGAAATCTACGAAAACCCCGAAAAATTCGACCCCTCTAGATGGGATAATCCAAAACGAAGAGCTGGATCATTCATTCCTTTCGGAGCAGGAATGAGGCTATGCCCTGGAATTGATCTTTCCAAGCTTGAAATTGCCATTTTTCTTCACTATTTCATCCATGGTGGCTTCAGACTTGAGAGAGTAAATCCAAATTGCCCAGAGAATTATCTGCCATTGCCAAGGCCTACAGACAACTGCCTTGCAAGAGTTGTAAGAGACTCATAAACGTTGAAATCAAAAGAGATCATATATGCATATTATATATATATAACG

Coding sequence (CDS)

ATGGAGGTTTGGGTATGGCTGGTCGGAGCTTTGGTCGGTGCATACATTTGTGTGTTTGGGATTCTCAGAAACCTTAATCAATGGCGGTTTGTCACCCGACACGGCGGCCGTTACAATCTCCCTCCCGGCGATATGGGCTGGCCGCTCGTCGGAACTCTTCTCCCCTTTCTTCAAGCTTTCCGATCGGGTCGACCCGATTCCTTCATTCATCACTTTGCCTCAAAGTATGGGAAAATAGGGATGTACAAGACGTACTTATTCGGGAGCCCAAGCGTGATCGTATGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGCCTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTCTCAACACCGTCTCCAGGCCCGAGCACCGCCGTATACGCCGCCTCATCGCCTCCCTAATCAGCGGCAACGATGCGCTGTCGATCTACATAGGTCACGTGGAGGGGATTGTGATCACGTGCCTAGAGGAGTGGGCCACCATGAAGAAGCCGGTCGAGTTCTTGTCGGAGATGAAAACGATGGCGTTTAAGATTTTGTTACACATATTCATCGGAGCTAATACGGCGGCGTTTATAGATAAGATGGAGAAGCTCTACACTGATTTCCACTTGGGGTTCATGTCCTCCGCCGTCGATATTCCCGGAACTACCTTCAGCCGAGCTCTCAAGGCAAGAAACGAGTTGATAAGGATCTTTGAAAGTGTTCTAAAGGAGAAGAGGGAGAATTTGAAGAACAAAGTGGAAGAAAACAGAAAAAAAGACATGACTGATTTGTTGTTGGAAGTTAAAGATGAAGAAGGCCATGGCTTGGATGATGGCTGCATTATTGATTTATTGATCGGTTTCTTCTTCGCTGGCCACGAAACCTCGGCTCATAGTATCATGAGGGCTATCATGTTTCTCTCGGAAAATCCCGAAACCCTTCGTAAAGCTAAGGCTGAGCAAGATCAAATTGTGAACGCAAGGCCAGCTAATGAACGACAAAGAGGCTTGACCATGAAGGAAATCAAACAAATGGAGTATCTTTCTAAGGTGATTGATGAGACATTGAGGAAAACGAGCTTGGCCTTCACACTATCGAGAGAGACGAGAAGCGACGTTAACTTAAATGGCTACACCATTCCGAAAGGATGGAAAATCCTTGTTTGGACGAGAGCAGTTCATATGGATCCTGAAATCTACGAAAACCCCGAAAAATTCGACCCCTCTAGATGGGATAATCCAAAACGAAGAGCTGGATCATTCATTCCTTTCGGAGCAGGAATGAGGCTATGCCCTGGAATTGATCTTTCCAAGCTTGAAATTGCCATTTTTCTTCACTATTTCATCCATGGTGGCTTCAGACTTGAGAGAGTAAATCCAAATTGCCCAGAGAATTATCTGCCATTGCCAAGGCCTACAGACAACTGCCTTGCAAGAGTTGTAAGAGACTCATAA

Protein sequence

MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAFRSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRILTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLSEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELIRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDNCLARVVRDS

Homology

[Close]

BLAST of Carg27401 vs. NCBI nr
Match: KAG7011523.1 (Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.3e-288
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. NCBI nr
Match: KAG6571838.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 997.7 bits (2578), Expect = 3.6e-287
Identity = 489/490 (99.80%), Postives = 489/490 (99.80%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. NCBI nr
Match: XP_022952525.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 997.7 bits (2578), Expect = 3.6e-287
Identity = 488/490 (99.59%), Postives = 489/490 (99.80%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARP+NER RGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. NCBI nr
Match: XP_023554757.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 989.6 bits (2557), Expect = 9.9e-285
Identity = 486/490 (99.18%), Postives = 487/490 (99.39%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAYI VF ILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYIFVFVILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETLRKAKAEQ+QIVNARPANER RGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQEQIVNARPANERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. NCBI nr
Match: XP_022971975.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 980.3 bits (2533), Expect = 6.0e-282
Identity = 479/490 (97.76%), Postives = 486/490 (99.18%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAY+ VFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFAS+YGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQ++IVNARP +ER RGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETR+DVNLNGYTIPKGWKILVWTRAVHMDPEIYENPE+FDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 252/494 (51.01%), Postives = 353/494 (71.46%), Query Frame = 0


Query: 1   MEV-WVWLVGA-LVGAYICVFGILRNLNQWRF-VTRHGGRYNLPPGDMGWPLVGTLLPFL 60
           MEV WV +  A L+  YI     +RNLN W + V      + LPPGDMGWPL+G LL F+
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFI 60

Query: 61  QAFRSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKA 120
           + F SG PDSFI++   KYG+ G+YKT+LFG+PS+IVC P++CR VL +D  F  GY K+
Sbjct: 61  KDFSSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKS 120

Query: 121 TRILTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPV 180
            + L   + +  VS  EHR  RRLI S I G+ AL++Y+  +E IVI  LEE ++MK PV
Sbjct: 121 IKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPV 180

Query: 181 EFLSEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKAR 240
           E L EMK ++FK ++H+F+G++    I K+   +TD + G  S  +++PG TF +AL+AR
Sbjct: 181 ELLKEMKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEAR 240

Query: 241 NELIRIFESVLKEKRENLKNKVEE-NRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFF 300
            +L +I + V+ E+R  ++N  +E +++KD+ D+LLEVKDE G  L+D  I DLLIG  F
Sbjct: 241 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLF 300

Query: 301 AGHETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSK 360
           AGHE++A S+M +I +L+++P  L+KAK EQ++I   R ++++Q  L++KEIKQM YLS+
Sbjct: 301 AGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQ--LSLKEIKQMVYLSQ 360

Query: 361 VIDETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSR 420
           VIDETLR  ++AF   RE  +DVN+NGY IPKGW++L+W RA+HMD E Y NPE+F+PSR
Sbjct: 361 VIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSR 420

Query: 421 WDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLP 480
           WD+   +AG+F+PFGAG RLCPG DL+KLEI+IFLHYF+   +RLER+NP C    LP+ 
Sbjct: 421 WDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVS 480

Query: 481 RPTDNCLARVVRDS 491
           +PTDNCLA+V++ S
Sbjct: 481 KPTDNCLAKVIKVS 491

[Close]

BLAST of Carg27401 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.2e-129
Identity = 227/470 (48.30%), Postives = 331/470 (70.43%), Query Frame = 0


Query: 21  ILRNLNQWRFVTRHG-GRYNLPPGDMGWPLVGTLLPFLQAFRSGRPDSFIHHFASKYGKI 80
           ++R +N W + +  G  R+ LPPGD+GWP +G +L FL+AF++  PDSF      +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  GMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRILTGSKALNTVSRPEHRRIR 140
           G+YK ++FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +S  EH+R+R
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLSEMKTMAFKILLHIFIGAN 200
           RL A+ ++G++ALS YI ++E  VIT L++W  M +  EFL+ ++ + F+I+++IF+ + 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 TAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELIRIFESVLKEKRENLKNKV 260
           +   +D +E+ YT  + G  + AV+IPG  + RALKAR  L+  F+S++ E+R   K  +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 261 EENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
             N KKDM D LL VKDE+G  LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 321 LRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETRSDV 380
           L++AKAEQ+ I+ +RP  E Q+GL++KE ++ME+LS+V+DETLR  + + T  RE ++DV
Sbjct: 325 LQRAKAEQEMILKSRP--EGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384

Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
            +NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN   PK  AG+F+PFGAG  L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444

Query: 441 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDNCLARV 487
           CPG DL+KLEI+IFLH+F+   ++++R NP CP  YLP  RPTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of Carg27401 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 1.8e-127
Identity = 220/487 (45.17%), Postives = 335/487 (68.79%), Query Frame = 0


Query: 3   VWVWLVGALVGAYICVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPLVGTLLPFLQAFR 62
           + +W    ++G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP++G +  FL+AF+
Sbjct: 7   ILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 66

Query: 63  SGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK ++FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 67  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 126

Query: 123 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 182
            G K+   +S  EH+R+RRL ++ ++G +ALS+YI  +E  V T LE+W+ M + +EFLS
Sbjct: 127 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 186

Query: 183 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 242
            ++ + FK++++IF+ + +   +D +E+ YT+ + G  +  +++PG  + RALKAR +L+
Sbjct: 187 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 246

Query: 243 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 302
             F+S++  +R   K  +  NR KDM D L++VKDE G  LDD  IIDLL+ +  AGHE+
Sbjct: 247 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 306

Query: 303 SAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L+KAK EQ++IV  R   ++   LT+KE ++M YLS+VIDET
Sbjct: 307 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK---LTLKETREMVYLSQVIDET 366

Query: 363 LRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNPK 422
           LR  + + T  RE +SDV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+   
Sbjct: 367 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 426

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP  +LP  RP DN
Sbjct: 427 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVVR 489
           CLAR+ R
Sbjct: 487 CLARITR 486

[Close]

BLAST of Carg27401 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-126
Identity = 224/490 (45.71%), Postives = 323/490 (65.92%), Query Frame = 0


Query: 6   WLVGALVG--AYICVFGILRNLNQWRFVTRHGG--RYNLPPGDMGWPLVGTLLPFLQAFR 65
           W V A+V   A + +   +R  + W +    G   R  LPPGDMGWPLVG +  FL+AF+
Sbjct: 7   WAVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK 66

Query: 66  SGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 125
           SGRPDSFI  FA ++G+ G+Y+ ++F SP+++   PE C+ VLM+D+ F  G+ KAT  L
Sbjct: 67  SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVAL 126

Query: 126 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATM--KKPVEF 185
            G K+   +   EHRR+R+L A+ I+G DAL+ Y+G ++  V+T L  W+        EF
Sbjct: 127 IGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEF 186

Query: 186 LSEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNE 245
           L+E++ M F+I++ IF+G        ++E+ YT+ + G  + A+D+PG  + +A++AR  
Sbjct: 187 LTELRRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHKAIRARRR 246

Query: 246 LIRIFESVLKEKR-ENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAG 305
           L+   + VL E+R    K         DM D L+ V+DE G  L D  IID+L+ +  AG
Sbjct: 247 LVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAG 306

Query: 306 HETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVI 365
           HE+S H  M A +FL ENPE L KAKAEQ+ I+ + P    Q+GLT+++ ++M YLS+V+
Sbjct: 307 HESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPG--QKGLTLRDFRKMAYLSQVV 366

Query: 366 DETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWD 425
           DETLR  +++F   R+   DV +NGY IPKGWK+ +W R+VHMDP++Y +P+KFDPSRW+
Sbjct: 367 DETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWE 426

Query: 426 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRP 485
            P  RAG+F+PFG G RLCPG DL+KLEI++FLH+F+  G++L R NPNC   YLP PRP
Sbjct: 427 GPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLL-GYKLTRKNPNCRVRYLPHPRP 486

Query: 486 TDNCLARVVR 489
            DNCLA++ R
Sbjct: 487 VDNCLAKITR 493

[Close]

BLAST of Carg27401 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 4.4e-126
Identity = 216/492 (43.90%), Postives = 329/492 (66.87%), Query Frame = 0


Query: 2   EVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGG--RYNLPPGDMGWPLVGTLLPFLQA 61
           ++WV L  A+V   + V  ++R  + W  V   G   R  LPPG+MGWP+VG++  FL+A
Sbjct: 16  DLWV-LAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRA 75

Query: 62  FRSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATR 121
           F+SG PD+FI  F  ++G+ G+Y+T++F SP+++   PE C+ VLM+DE F  G+ KAT 
Sbjct: 76  FKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 122 ILTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKK-PVE 181
            L G K+   +S  +HRRIR+L A+ I+G DAL+ Y+  ++  V+  L  W++ +   VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 182 FLSEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARN 241
           FL+E++ M FKI++ IF+     A ++ +E+ YTD + G  + A+++PG  + RAL+AR 
Sbjct: 196 FLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255

Query: 242 ELIRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAG 301
           +L+ + + VL  +R       + +   DM D L+E +DE G  L D  I+D+LI +  AG
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315

Query: 302 HETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVI 361
           HE+S H  M A +FL ENP+   +AKAEQ++I+ + PA   Q GLT+++ K+M +LS+V+
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPAT--QNGLTLRDFKKMHFLSQVV 375

Query: 362 DETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWD 421
           DETLR  +++F   R+   D+ +NGY IPKGWK+ +W R+VHMD ++Y +P+ F+PSRW+
Sbjct: 376 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 435

Query: 422 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRP 481
            P  +AG+F+PFG G RLCPG DL+KLEI++FLH+F+  G++L+R NP C   YLP PRP
Sbjct: 436 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRANPKCRVRYLPHPRP 495

Query: 482 TDNCLARVVRDS 491
            DNCLA + + S
Sbjct: 496 VDNCLATITKVS 503

[Close]

BLAST of Carg27401 vs. ExPASy TrEMBL
Match: A0A6J1GM12 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 1.8e-287
Identity = 488/490 (99.59%), Postives = 489/490 (99.80%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARP+NER RGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. ExPASy TrEMBL
Match: A0A6J1I785 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 2.9e-282
Identity = 479/490 (97.76%), Postives = 486/490 (99.18%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           MEVWVWLVGALVGAY+ VFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF
Sbjct: 1   MEVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHFAS+YGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQ++IVNARP +ER RGLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRETR+DVNLNGYTIPKGWKILVWTRAVHMDPEIYENPE+FDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

[Close]

BLAST of Carg27401 vs. ExPASy TrEMBL
Match: A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.1e-260
Identity = 434/490 (88.57%), Postives = 468/490 (95.51%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           M++W+ L+GAL G Y+ VFGILRNLNQWRFVTRHG RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 3   MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFHLGFMS+AVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKRENLKNKV ENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETL KAKAEQ++IVNARPA+ + +GLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP +FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492

[Close]

BLAST of Carg27401 vs. ExPASy TrEMBL
Match: A0A6J1ET68 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.5e-259
Identity = 433/490 (88.37%), Postives = 467/490 (95.31%), Query Frame = 0


Query: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
           M++W+ L+GAL G Y+ +FGILRNLNQWRFVTRHG RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 3   MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
           LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YI HVEGIV+TCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFH+GFMS+AVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKRENLKNKV ENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETL KAKAEQ+QIVNARPA+ + +GLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492

[Close]

BLAST of Carg27401 vs. ExPASy TrEMBL
Match: A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.0e-259
Identity = 438/489 (89.57%), Postives = 467/489 (95.50%), Query Frame = 0


Query: 2   EVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAFR 61
           ++W+ L+GALV +YI VF ILRNLNQW FVTRHG RYNLPPGDMGWPL+GTLLPFLQAFR
Sbjct: 3   QLWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFR 62

Query: 62  SGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 121
           SGRPDSFIH FA KYGKIGMYKTYLFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRIL
Sbjct: 63  SGRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRIL 122

Query: 122 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 181
           TGSKALNTV +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEWA+MKKPVEFLS
Sbjct: 123 TGSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLS 182

Query: 182 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 241
           EMKT+AFK+LLHIFIGANTAAFID+MEKLY DFHLGFMSS VD+PGTTFSRALKARNELI
Sbjct: 183 EMKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELI 242

Query: 242 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 301
           +IFE+VLKEKRENLK KV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHET
Sbjct: 243 KIFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302

Query: 302 SAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDET 361
           SAHSIMRAIMFLSENPETL+KAKAEQ+QIV ARPA+ + +GLTMKEIKQMEYL KVIDET
Sbjct: 303 SAHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDET 362

Query: 362 LRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNPK 421
           LRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP+KFDPSRW+NPK
Sbjct: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPK 422

Query: 422 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 481
           RR GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLPRPTDN
Sbjct: 423 RRGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482

Query: 482 CLARVVRDS 491
           CLARVVRDS
Sbjct: 483 CLARVVRDS 491

[Close]

BLAST of Carg27401 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.0e-130
Identity = 227/470 (48.30%), Postives = 331/470 (70.43%), Query Frame = 0


Query: 21  ILRNLNQWRFVTRHG-GRYNLPPGDMGWPLVGTLLPFLQAFRSGRPDSFIHHFASKYGKI 80
           ++R +N W + +  G  R+ LPPGD+GWP +G +L FL+AF++  PDSF      +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  GMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRILTGSKALNTVSRPEHRRIR 140
           G+YK ++FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +S  EH+R+R
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLSEMKTMAFKILLHIFIGAN 200
           RL A+ ++G++ALS YI ++E  VIT L++W  M +  EFL+ ++ + F+I+++IF+ + 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 TAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELIRIFESVLKEKRENLKNKV 260
           +   +D +E+ YT  + G  + AV+IPG  + RALKAR  L+  F+S++ E+R   K  +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 261 EENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
             N KKDM D LL VKDE+G  LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 321 LRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETRSDV 380
           L++AKAEQ+ I+ +RP  E Q+GL++KE ++ME+LS+V+DETLR  + + T  RE ++DV
Sbjct: 325 LQRAKAEQEMILKSRP--EGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384

Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
            +NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN   PK  AG+F+PFGAG  L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444

Query: 441 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDNCLARV 487
           CPG DL+KLEI+IFLH+F+   ++++R NP CP  YLP  RPTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of Carg27401 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 220/487 (45.17%), Postives = 335/487 (68.79%), Query Frame = 0


Query: 3   VWVWLVGALVGAYICVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPLVGTLLPFLQAFR 62
           + +W    ++G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP++G +  FL+AF+
Sbjct: 7   ILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 66

Query: 63  SGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK ++FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 67  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 126

Query: 123 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 182
            G K+   +S  EH+R+RRL ++ ++G +ALS+YI  +E  V T LE+W+ M + +EFLS
Sbjct: 127 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 186

Query: 183 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 242
            ++ + FK++++IF+ + +   +D +E+ YT+ + G  +  +++PG  + RALKAR +L+
Sbjct: 187 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 246

Query: 243 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 302
             F+S++  +R   K  +  NR KDM D L++VKDE G  LDD  IIDLL+ +  AGHE+
Sbjct: 247 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 306

Query: 303 SAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L+KAK EQ++IV  R   ++   LT+KE ++M YLS+VIDET
Sbjct: 307 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK---LTLKETREMVYLSQVIDET 366

Query: 363 LRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNPK 422
           LR  + + T  RE +SDV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+   
Sbjct: 367 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 426

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP  +LP  RP DN
Sbjct: 427 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVVR 489
           CLAR+ R
Sbjct: 487 CLARITR 486

[Close]

BLAST of Carg27401 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 220/487 (45.17%), Postives = 335/487 (68.79%), Query Frame = 0


Query: 3   VWVWLVGALVGAYICVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPLVGTLLPFLQAFR 62
           + +W    ++G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP++G +  FL+AF+
Sbjct: 7   ILMWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 66

Query: 63  SGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK ++FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 67  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 126

Query: 123 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 182
            G K+   +S  EH+R+RRL ++ ++G +ALS+YI  +E  V T LE+W+ M + +EFLS
Sbjct: 127 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 186

Query: 183 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 242
            ++ + FK++++IF+ + +   +D +E+ YT+ + G  +  +++PG  + RALKAR +L+
Sbjct: 187 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 246

Query: 243 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 302
             F+S++  +R   K  +  NR KDM D L++VKDE G  LDD  IIDLL+ +  AGHE+
Sbjct: 247 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 306

Query: 303 SAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPANERQRGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L+KAK EQ++IV  R   ++   LT+KE ++M YLS+VIDET
Sbjct: 307 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK---LTLKETREMVYLSQVIDET 366

Query: 363 LRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNPK 422
           LR  + + T  RE +SDV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+   
Sbjct: 367 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 426

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP  +LP  RP DN
Sbjct: 427 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVVR 489
           CLAR+ R
Sbjct: 487 CLARITR 486

[Close]

BLAST of Carg27401 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-60
Identity = 149/452 (32.96%), Postives = 233/452 (51.55%), Query Frame = 0


Query: 37  RYNLPPGDMGWPLVGTLLPFLQAFRSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCV 96
           R  LPPG MG P +G  L       +  P+SF     +KYG I  +KT++ G P V++  
Sbjct: 46  RLRLPPGSMGLPYIGETL----RLYTENPNSFFATRQNKYGDI--FKTHILGCPCVMISS 105

Query: 97  PEVCRHVLMNDEQ-FAFGYSKATRILTGSKALNTVSRPEHRRIRRLIASLISGNDALSIY 156
           PE  R VL++    F   Y  +   + G +AL     P H  ++RL+ S    + AL   
Sbjct: 106 PEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRPT 165

Query: 157 IGHVEGIVITCLEEWATMKKPVEFLSEMKTMAFKI-LLHIFIGANTAAFIDKMEKLYTDF 216
           + H+E +V+  L  W T +K +  L  MK  AF + ++  F        ID ++ LY   
Sbjct: 166 VSHIELLVLQTLSSW-TSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRL 225

Query: 217 HLGFMSSAVDIPGTTFSRALKARNELIRIFESVLKEKRENLKNKVEENRKKDMTDLLLEV 276
             G+ S  +D+PGT F +++KAR EL      V++++REN         +  +  +LL  
Sbjct: 226 ERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRREN------GREEGGLLGVLLGA 285

Query: 277 KDEEGHGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNAR 336
           KD++ +GL D  I D +IG  FA  +T+A  +   + +L ++P  L++   EQ  I   +
Sbjct: 286 KDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSI--RQ 345

Query: 337 PANERQRGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILV 396
              +  R ++ ++ ++M   ++VI ETLR  S+     RE   DV  +GY IPKGWK+L 
Sbjct: 346 KIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLP 405

Query: 397 WTRAVHMDPEIYENPEKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYF 456
             R +H   E + +PEKFDPSR++    +  +++PFG G+  CPG +L+KLE+ I LH+ 
Sbjct: 406 LFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHH- 465

Query: 457 IHGGFRLERVNPNCPENYLPLPRPTDNCLARV 487
           +   FR E +       Y P P P      RV
Sbjct: 466 LTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

[Close]

BLAST of Carg27401 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-60
Identity = 149/452 (32.96%), Postives = 233/452 (51.55%), Query Frame = 0


Query: 37  RYNLPPGDMGWPLVGTLLPFLQAFRSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCV 96
           R  LPPG MG P +G  L       +  P+SF     +KYG I  +KT++ G P V++  
Sbjct: 46  RLRLPPGSMGLPYIGETL----RLYTENPNSFFATRQNKYGDI--FKTHILGCPCVMISS 105

Query: 97  PEVCRHVLMNDEQ-FAFGYSKATRILTGSKALNTVSRPEHRRIRRLIASLISGNDALSIY 156
           PE  R VL++    F   Y  +   + G +AL     P H  ++RL+ S    + AL   
Sbjct: 106 PEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRPT 165

Query: 157 IGHVEGIVITCLEEWATMKKPVEFLSEMKTMAFKI-LLHIFIGANTAAFIDKMEKLYTDF 216
           + H+E +V+  L  W T +K +  L  MK  AF + ++  F        ID ++ LY   
Sbjct: 166 VSHIELLVLQTLSSW-TSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRL 225

Query: 217 HLGFMSSAVDIPGTTFSRALKARNELIRIFESVLKEKRENLKNKVEENRKKDMTDLLLEV 276
             G+ S  +D+PGT F +++KAR EL      V++++REN         +  +  +LL  
Sbjct: 226 ERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRREN------GREEGGLLGVLLGA 285

Query: 277 KDEEGHGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLRKAKAEQDQIVNAR 336
           KD++ +GL D  I D +IG  FA  +T+A  +   + +L ++P  L++   EQ  I   +
Sbjct: 286 KDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSI--RQ 345

Query: 337 PANERQRGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILV 396
              +  R ++ ++ ++M   ++VI ETLR  S+     RE   DV  +GY IPKGWK+L 
Sbjct: 346 KIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLP 405

Query: 397 WTRAVHMDPEIYENPEKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYF 456
             R +H   E + +PEKFDPSR++    +  +++PFG G+  CPG +L+KLE+ I LH+ 
Sbjct: 406 LFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHH- 465

Query: 457 IHGGFRLERVNPNCPENYLPLPRPTDNCLARV 487
           +   FR E +       Y P P P      RV
Sbjct: 466 LTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:

BLAST of Carg27401 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011523.1	4.3e-288	100.00	Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6571838.1	3.6e-287	99.80	Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022952525.1	3.6e-287	99.59	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_023554757.1	9.9e-285	99.18	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971975.1	6.0e-282	97.76	beta-amyrin 11-oxidase-like [Cucurbita maxima]	[more]

BLAST of Carg27401 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.6e-141	51.01	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	4.2e-129	48.30	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	1.8e-127	45.17	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q9AXH9	1.1e-126	45.71	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	4.4e-126	43.90	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]

BLAST of Carg27401 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GM12	1.8e-287	99.59	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 S...	[more]
A0A6J1I785	2.9e-282	97.76	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=...	[more]
A0A6J1K3T5	4.1e-260	88.57	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=...	[more]
A0A6J1ET68	1.5e-259	88.37	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 S...	[more]
A0A5A7TAG0	2.0e-259	89.57	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]

BLAST of Carg27401 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	3.0e-130	48.30	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	1.3e-128	45.17	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.3e-128	45.17	ent-kaurenoic acid hydroxylase 2	[more]
AT2G29090.1	9.5e-60	32.96	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	9.5e-60	32.96	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 241..265
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 19..467
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 19..467
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 437..460 score: 22.62 coord: 306..332 score: 23.98 coord: 395..419 score: 28.48 coord: 427..437 score: 56.24 coord: 286..303 score: 28.65
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 297..314 score: 29.48 coord: 437..448 score: 31.75 coord: 428..437 score: 57.97 coord: 356..367 score: 36.49
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 41..456 e-value: 2.6E-71 score: 240.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..488 e-value: 7.8E-105 score: 353.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 37..487
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 430..439

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27401-RA	Carg27401-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen