Carg27378 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27378
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pectinesterase inhibitor-like
Location	Carg_Chr10: 9269965 .. 9270558 (+)
RNA-Seq Expression	Carg27378
Synteny	Carg27378

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

mRNA sequence

ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

Coding sequence (CDS)

ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

Protein sequence

MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANILSQGNRNMNSEDIFNF

Homology

[Close]

BLAST of Carg27378 vs. NCBI nr
Match: XP_022961546.1 (pectinesterase inhibitor-like [Cucurbita moschata] >KAG6590451.1 hypothetical protein SDJN03_15874, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023986.1 hypothetical protein SDJN02_15014, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.6 bits (1002), Expect = 8.1e-105
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]

BLAST of Carg27378 vs. NCBI nr
Match: XP_023515956.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388.7 bits (997), Expect = 3.1e-104
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAITILSITHFQILAD+PQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITILSITHFQILADDPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]

BLAST of Carg27378 vs. NCBI nr
Match: XP_022968792.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 384.4 bits (986), Expect = 5.8e-103
Identity = 192/197 (97.46%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAIT+LS+THFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITVLSMTHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQN TEMDTFMDECLTDCLE+YQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNNTEMDTFMDECLTDCLENYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAM N
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMTN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]

BLAST of Carg27378 vs. NCBI nr
Match: XP_038880337.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 242.3 bits (617), Expect = 3.6e-60
Identity = 122/189 (64.55%), Postives = 152/189 (80.42%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK ISI  M + +LS+  FQIL+  PQ P  +   DLITKTCSSTPYND+C SIL SSPN
Sbjct: 1   MKPISI-VMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           SNG DLYGL +I++ IAAENVS I+E + +LQN T +D+F+D+CLTDCLESYQDA DQIE
Sbjct: 61  SNGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLDDCLTDCLESYQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+KTWV+AAM+D A C+ GFKEKP ++SPI +M+ VFDQIC+IIL+M 
Sbjct: 121 DSVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMT 180

Query: 181 NILSQGNRN 189
            +LS+   N
Sbjct: 181 QLLSEEKIN 188

[Close]

BLAST of Carg27378 vs. NCBI nr
Match: XP_011659828.1 (putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein Csa_007292 [Cucumis sativus])

HSP 1 Score: 232.3 bits (591), Expect = 3.7e-57
Identity = 114/189 (60.32%), Postives = 154/189 (81.48%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLI+KTCSST Y+++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ +AA+NVS I+E++ +LQN T +D+F+D CLTDCLES+QDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+KTW++AAM+D A C++GFKEK  ++SPI +M+ VFDQICSIIL++ 
Sbjct: 121 DSVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSIN 180

Query: 181 NILSQGNRN 189
            +LSQGN N
Sbjct: 181 QLLSQGNTN 188

[Close]

BLAST of Carg27378 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 48/159 (30.19%), Postives = 82/159 (51.57%), Query Frame = 0


Query: 30  FLTNADLITKTCSSTPYN------DLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLI 89
           FL +AD++  TC            D C+  L + P S+  DL GL  I   +A +  S I
Sbjct: 11  FLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKI 70

Query: 90  HEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMND 149
              +  +  K+++D  + + L DC+  Y DA   ++++I   + K Y      ++AA++D
Sbjct: 71  QTFIGRIL-KSKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDD 130

Query: 150 GAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANIL 183
              CE+GFKEK    SP+ + +K + Q+ +I LA+  +L
Sbjct: 131 SVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of Carg27378 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-13
Identity = 46/156 (29.49%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 33  NADLITKTCSSTPYN------DLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEH 92
           +AD++  TC            D C+  L + P S+  DL GL  I   +A ++ S I   
Sbjct: 23  SADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTF 82

Query: 93  LMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAV 152
           +  +  K+++D  + + L DC+  Y DA   ++++I   + K Y      ++AA++D   
Sbjct: 83  IGRIL-KSKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVT 142

Query: 153 CENGFKEKPDHKSPIVEMSKVFDQICSIILAMANIL 183
           CE+GFKEK    SP+ + +K + Q+ +I LA+  +L
Sbjct: 143 CEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Carg27378 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.0e-06
Identity = 34/125 (27.20%), Postives = 62/125 (49.60%), Query Frame = 0


Query: 33  NADLITKTCSSTPYNDLCLSILESSP---NSNGTDLYGLTEIMLYIAAENVSLIHEHLME 92
           NA  IT +C  TPY  +C   + +SP     + TD +   ++++    +    +H  +  
Sbjct: 31  NAHFIT-SCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSS 90

Query: 93  LQNKTEMDTFMDECLTDCLESYQDATDQIEDSITAL-EFKAYHDIKTWVAAAMNDGAVCE 152
           L+    +       L DCLE Y+D  DQ+  S  +  ++ + HD +T ++AA+ +   C 
Sbjct: 91  LKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCR 150

Query: 153 NGFKE 154
           NGF++
Sbjct: 151 NGFRD 154

[Close]

BLAST of Carg27378 vs. ExPASy TrEMBL
Match: A0A6J1HAN5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111462095 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 3.9e-105
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]

BLAST of Carg27378 vs. ExPASy TrEMBL
Match: A0A6J1HUH7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467924 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.8e-103
Identity = 192/197 (97.46%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAIT+LS+THFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITVLSMTHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQN TEMDTFMDECLTDCLE+YQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNNTEMDTFMDECLTDCLENYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAM N
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMTN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]

BLAST of Carg27378 vs. ExPASy TrEMBL
Match: A0A0A0M1H8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.8e-57
Identity = 114/189 (60.32%), Postives = 154/189 (81.48%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLI+KTCSST Y+++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ +AA+NVS I+E++ +LQN T +D+F+D CLTDCLES+QDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+KTW++AAM+D A C++GFKEK  ++SPI +M+ VFDQICSIIL++ 
Sbjct: 121 DSVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSIN 180

Query: 181 NILSQGNRN 189
            +LSQGN N
Sbjct: 181 QLLSQGNTN 188

[Close]

BLAST of Carg27378 vs. ExPASy TrEMBL
Match: A0A5A7UBK3 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004170 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 4.0e-57
Identity = 117/189 (61.90%), Postives = 149/189 (78.84%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLITKTCSSTP+ ++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ IAA NVS I+E + +LQN T +D+F+D CLTDCLESYQDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+ TWV+AAM+D A C+ GFKEK  ++SPI +M+ VFDQIC IILA+ 
Sbjct: 121 DSVTALEFKAYNDVNTWVSAAMSDAATCDMGFKEKQGYQSPIAQMTTVFDQICKIILAIN 180

Query: 181 NILSQGNRN 189
            +LSQGN N
Sbjct: 181 KLLSQGNSN 188

[Close]

BLAST of Carg27378 vs. ExPASy TrEMBL
Match: A0A6J1I2M6 (pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 6.0e-53
Identity = 108/191 (56.54%), Postives = 145/191 (75.92%), Query Frame = 0


Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MK IS+A   + ++S+  FQ   DN         +LITK CS T YNDLC +IL+++PNS
Sbjct: 1   MKPISVAVAVLVVVSLCSFQAQGDN---------ELITKACSRTAYNDLCKAILQAAPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           N  DLYGLT+  L IAA+NVS I++ + +LQN + +D+FMDECLTDCLE+YQDATDQIED
Sbjct: 61  NDADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           S+TALEFKA++D++TWVAAAM+D A C+ GFK+KP ++SP+ +MS VFDQICSI+L   +
Sbjct: 121 SVTALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFNH 180

Query: 181 ILSQGNRNMNS 192
            L +GN ++ S
Sbjct: 181 CLIEGNVHLTS 182

[Close]

BLAST of Carg27378 vs. TAIR 10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 56/193 (29.02%), Postives = 95/193 (49.22%), Query Frame = 0


Query: 3   LISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNSNG 62
           L+ ++ +A+T  + TH       P  P +++A L    CS T Y + C + L SS N + 
Sbjct: 85  LLILSVVALTAGAFTH-----SPPHHPPISSASL-KSLCSVTRYPETCFNSLSSSLNESD 144

Query: 63  TDL--YGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 122
           + L    + E+ L +AA+ +S +      + +  E     D  + DC++ Y DA  Q+ D
Sbjct: 145 SKLNPESILELSLRVAAKEISNLSISFRSINDMPE-----DAAVGDCVKLYTDALSQLND 204

Query: 123 SITALEFK------------AYHDIKTWVAAAMNDGAVCENGFKEKPD-HKSPIVEMSKV 181
           SIT +E K               D+KTW++AAM DG  C +G +E      + I +  ++
Sbjct: 205 SITEIERKKKKGGNNWLTEEVVGDVKTWISAAMTDGETCSDGIEEMGTIVGNEIKKKMEM 264

[Close]

BLAST of Carg27378 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.9e-12
Identity = 51/172 (29.65%), Postives = 84/172 (48.84%), Query Frame = 0


Query: 32  TNADLITKTCSST-------PYNDLCLSILESSPNS--NGTDLYGLTEIMLYIAAENVSL 91
           + +D+I +TC S         YN  C+S L +SP +  + T+L  L  + +  A +N + 
Sbjct: 21  STSDMIDQTCKSCAAKSTIFDYN-FCVSSLNNSPIALPSPTNLSSLALVPMLQALDNATA 80

Query: 92  IHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITA-LEFKAYHDIKTWVAAAM 151
               + +L    +  +F   CL DCLE Y+DATD++E+++   +  K    +   V+AAM
Sbjct: 81  TASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRKELGTVNVMVSAAM 140

Query: 152 NDGAVCENGFKEKPD----------HKSPIVEMSKVFDQICSIILAMANILS 184
                CENGF+E+ D            SPI + +    +   I L + N+LS
Sbjct: 141 ESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIALCIFNMLS 191

[Close]

BLAST of Carg27378 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-11
Identity = 39/154 (25.32%), Postives = 83/154 (53.90%), Query Frame = 0


Query: 33  NADLITKTCSSTPYNDLCLSILESSPNSN---GTDLYGLTEIMLYIAAENVSLIHEHLME 92
           N+ +I+  C  +   +LCLS + S P S    GT    +  I +  A+ N S    ++ +
Sbjct: 23  NSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTS-NQIEIIAISAASANASATSSYIKQ 82

Query: 93  LQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCEN 152
             +  +++  +++ L DC + YQDA +Q++DSI+A+   A+ D+  W++AA++    C +
Sbjct: 83  KLSNEDLEPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTDVDVWLSAAISAIESCGS 142

Query: 153 GFKEKPDHKSPIVEMSKVFDQICSIILAMANILS 184
               +  + + + + ++VF ++C   L +  +L+
Sbjct: 143 ALGSRAGNDAELSQRNEVFLKLCKNALMINKMLT 175

[Close]

BLAST of Carg27378 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.7e-10
Identity = 34/134 (25.37%), Postives = 67/134 (50.00%), Query Frame = 0


Query: 50  CLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLE 109
           C++ L S P +       L  I L IA+ N S    ++     +  ++  +++ L DC +
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCSK 85

Query: 110 SYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFD 169
           +Y DA  Q++DS+ AL   ++ D+  W+  A++DG  CEN   ++  + + +   +    
Sbjct: 86  NYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNLL 145

Query: 170 QICSIILAMANILS 184
           ++C   L +  IL+
Sbjct: 146 KLCKDALLINTILT 159

[Close]

BLAST of Carg27378 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-08
Identity = 46/174 (26.44%), Postives = 84/174 (48.28%), Query Frame = 0


Query: 13  ILSITHFQILAD---NPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS-NGTDLYGL 72
           +L +  F +L++   N Q     +    T T     YN LC++ LE++P +    DL GL
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYN-LCVTSLETNPQAKTAKDLAGL 63

Query: 73  TEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFK 132
                  A    + +   + ++    +++      L DCL+ Y DA   + +++  ++ +
Sbjct: 64  VMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSR 123

Query: 133 AYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANIL 183
            Y  +KT ++AAM+  + CE GFKE+    SP+ + +    Q+  I LA  N+L
Sbjct: 124 NYPTVKTVLSAAMDTPSTCETGFKER-KAPSPVTKENDNLYQMILIPLAFTNML 175

The following BLAST results are available for this feature:

BLAST of Carg27378 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961546.1	8.1e-105	100.00	pectinesterase inhibitor-like [Cucurbita moschata] >KAG6590451.1 hypothetical pr...	[more]
XP_023515956.1	3.1e-104	99.49	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968792.1	5.8e-103	97.46	pectinesterase inhibitor-like [Cucurbita maxima]	[more]
XP_038880337.1	3.6e-60	64.55	putative invertase inhibitor [Benincasa hispida]	[more]
XP_011659828.1	3.7e-57	60.32	putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein ...	[more]

BLAST of Carg27378 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9YUH4	2.4e-14	30.19	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	3.4e-13	29.49	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
O49298	4.0e-06	27.20	Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Carg27378 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HAN5	3.9e-105	100.00	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111462095 PE=4...	[more]
A0A6J1HUH7	2.8e-103	97.46	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467924 PE=4 S...	[more]
A0A0A0M1H8	1.8e-57	60.32	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 S...	[more]
A0A5A7UBK3	4.0e-57	61.90	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1I2M6	6.0e-53	56.54	pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4...	[more]

BLAST of Carg27378 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47670.1	1.7e-12	29.02	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	3.9e-12	29.65	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G55770.1	1.5e-11	25.32	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	4.7e-10	25.37	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	2.0e-08	26.44	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..178 e-value: 1.7E-33 score: 127.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..178 e-value: 2.7E-31 score: 109.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..182 e-value: 2.6E-38 score: 129.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..182 e-value: 5.0E-40 score: 138.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 35..183
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 31..182
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 31..182
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 36..182 e-value: 6.62237E-54 score: 167.123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27378-RA	Carg27378-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity