Carg27339 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27339
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCarg_Chr04: 15498306 .. 15498942 (+)
RNA-Seq ExpressionCarg27339
SyntenyCarg27339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCGTACGTCTCTCATCCCCTTTTCGATTAACTTTCAGATCATTCGACATAAATTCTCGATGATGTGCGCAGTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGGTTTGCTACTTTACAATGAATTTTCATAATTAAATTCCTATTTTTTCAAAAGGAAAATTTGAAAATAGTATTGATGTTTACCTAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

mRNA sequence

ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

Coding sequence (CDS)

ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

Protein sequence

MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCLT
Homology
BLAST of Carg27339 vs. NCBI nr
Match: KAG6601796.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032510.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 312.8 bits (800), Expect = 1.8e-81
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCLT 161
           DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCLT
Sbjct: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCLT 160

BLAST of Carg27339 vs. NCBI nr
Match: XP_022963468.1 (uncharacterized protein LOC111463790 [Cucurbita moschata])

HSP 1 Score: 306.6 bits (784), Expect = 1.3e-79
Identity = 157/159 (98.74%), Postives = 158/159 (99.37%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 159

BLAST of Carg27339 vs. NCBI nr
Match: XP_023538969.1 (uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 3.4e-77
Identity = 154/159 (96.86%), Postives = 155/159 (97.48%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           DFPELEEIKSVLSSRFG EFAARAVELR NNTVNHS+ L
Sbjct: 121 DFPELEEIKSVLSSRFGREFAARAVELRINNTVNHSIML 159

BLAST of Carg27339 vs. NCBI nr
Match: XP_023522757.1 (uncharacterized protein LOC111786761, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296.2 bits (757), Expect = 1.7e-76
Identity = 153/156 (98.08%), Postives = 153/156 (98.08%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHS 157
           DFPELEEIKSVLSSRFG EFAARAVELR NNTVNHS
Sbjct: 121 DFPELEEIKSVLSSRFGREFAARAVELRINNTVNHS 156

BLAST of Carg27339 vs. NCBI nr
Match: XP_022971780.1 (uncharacterized protein LOC111470458 [Cucurbita maxima])

HSP 1 Score: 292.4 bits (747), Expect = 2.4e-75
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKL HGRYVEDRKD ARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLHHGRYVEDRKDTARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLY-LLAPGRECPKELEEAVSSVVFAASRC 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRER++ LLAPGRECPKELEEAVSSVVFAASRC
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERMHLLLAPGRECPKELEEAVSSVVFAASRC 120

Query: 121 EDFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           EDFPEL+EIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 EDFPELQEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 160

BLAST of Carg27339 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-06
Identity = 30/111 (27.03%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 27  ALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLN 86
           A+  ++ LK  +     +++++ A LL +   + A  R E + R + L++ + ++E    
Sbjct: 20  AVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQIIEVLCE 79

Query: 87  LLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFG 138
           LL  R+ L+    E P E++E++ ++V+++ R +  PELE+IK+ L +++G
Sbjct: 80  LLHARINLINATTEMPLEMKESIFTLVYSSQRIQ-IPELEQIKNQLKAKYG 129

BLAST of Carg27339 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0

Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

BLAST of Carg27339 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0

Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

BLAST of Carg27339 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0

Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

BLAST of Carg27339 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.9e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0

Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

BLAST of Carg27339 vs. ExPASy TrEMBL
Match: A0A6J1HHW4 (uncharacterized protein LOC111463790 OS=Cucurbita moschata OX=3662 GN=LOC111463790 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.1e-80
Identity = 157/159 (98.74%), Postives = 158/159 (99.37%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 159

BLAST of Carg27339 vs. ExPASy TrEMBL
Match: A0A6J1I6P0 (uncharacterized protein LOC111470458 OS=Cucurbita maxima OX=3661 GN=LOC111470458 PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.2e-75
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0

Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKL HGRYVEDRKD ARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLHHGRYVEDRKDTARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLY-LLAPGRECPKELEEAVSSVVFAASRC 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRER++ LLAPGRECPKELEEAVSSVVFAASRC
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERMHLLLAPGRECPKELEEAVSSVVFAASRC 120

Query: 121 EDFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           EDFPEL+EIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 EDFPELQEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 160

BLAST of Carg27339 vs. ExPASy TrEMBL
Match: A0A6J1CEI4 (uncharacterized protein LOC111010490 OS=Momordica charantia OX=3673 GN=LOC111010490 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.9e-45
Identity = 99/153 (64.71%), Postives = 121/153 (79.08%), Query Frame = 0

Query: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLL-HEGLPKLA 63
           +RR+  PLLGR+ +SS+FRRL+ +A   L++L   R  RY +DR D  RLL  +GL  LA
Sbjct: 1   MRRILNPLLGRTFKSSNFRRLIHSAFTRLQSLNTHRRRRYSQDRDDVVRLLQQDGLHNLA 60

Query: 64  LSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCED 123
           LSRCEQ+ +HQNL+DAYGM+EGYLNLL +R+YLL   RECP EL+EA S VV+AASRC D
Sbjct: 61  LSRCEQVIKHQNLLDAYGMIEGYLNLLMDRIYLLVQQRECPDELKEAASGVVYAASRCRD 120

Query: 124 FPELEEIKSVLSSRFGTEFAARAVELRNNNTVN 155
           FPELEEIKSVL+SRFG EF+ RAVELRNN+ VN
Sbjct: 121 FPELEEIKSVLTSRFGREFSGRAVELRNNSGVN 153

BLAST of Carg27339 vs. ExPASy TrEMBL
Match: A0A1S3BD77 (uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 8.6e-42
Identity = 95/155 (61.29%), Postives = 117/155 (75.48%), Query Frame = 0

Query: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63
           +RR+   ++GRS RSS F+RLVQ++   L++L   R  RY ED  D   LL +GL KLAL
Sbjct: 1   MRRILNRVVGRSFRSSGFKRLVQSSFTRLQSLTDHRRRRYSEDLNDVLSLLQDGLHKLAL 60

Query: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123
           SRCEQM +HQNL+DAYG++EGYLNLL ER+ LL   RECP EL+EA SSVVFAASR +DF
Sbjct: 61  SRCEQMIKHQNLLDAYGLIEGYLNLLMERIQLLGRARECPDELKEAASSVVFAASRWKDF 120

Query: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSV 158
            EL +IKS+ +S+FG EF A AVELRNNN VN S+
Sbjct: 121 TELGDIKSIFTSQFGKEFTAPAVELRNNNRVNQSI 155

BLAST of Carg27339 vs. ExPASy TrEMBL
Match: A0A0A0K949 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.8e-40
Identity = 92/155 (59.35%), Postives = 119/155 (76.77%), Query Frame = 0

Query: 4   LRRVFKPLLGRSRSSS-FRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63
           +R++ K +LGRS SSS F+R VQ++   L++L   R  R+ ED  +   LL E L +LAL
Sbjct: 1   MRKIMKRVLGRSFSSSCFKRSVQSSFTRLKSLTDHRRRRFSEDLNEVVSLLQEDLQELAL 60

Query: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123
           SRCEQM +HQNL+DAYG++EGYLNLL ER++LL   RECP EL+EAVSSV+FAASR +DF
Sbjct: 61  SRCEQMIKHQNLVDAYGLIEGYLNLLIERIHLLGRERECPDELKEAVSSVIFAASRWKDF 120

Query: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSV 158
            EL ++KS+ +S+FG EF ARAVELRNNN VN S+
Sbjct: 121 TELGDVKSIFTSQFGKEFTARAVELRNNNRVNQSI 155

BLAST of Carg27339 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 110.9 bits (276), Expect = 9.4e-25
Identity = 63/148 (42.57%), Postives = 92/148 (62.16%), Query Frame = 0

Query: 11  LLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMF 70
           LLGRS +++ F+ L+  AL  L  LK  R  R  +   D   LL  G  + A  R +Q+ 
Sbjct: 8   LLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAYHRVDQVV 67

Query: 71  RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIK 130
           + QN +D    + GY  L  +R++L    R+CP+EL EAVS ++FAASR  +FPEL+EI+
Sbjct: 68  KDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEFPELQEIR 127

Query: 131 SVLSSRFGTEFAARAVELRNNNTVNHSV 158
           +VL SRFG + AAR++ELR+N  V+  +
Sbjct: 128 NVLISRFGKDLAARSIELRSNCGVDPKI 155

BLAST of Carg27339 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-17
Identity = 45/142 (31.69%), Postives = 84/142 (59.15%), Query Frame = 0

Query: 16  RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLM 75
           +++  + L++  +  ++ ++  R  +  + R++ A+LL  G    A  R E + R + +M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 76  DAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSR 135
            A  ++E +  L+  RL ++   RECP +L+EA+SSV FAA RC D  EL++++ +  S+
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 136 FGTEFAARAVELRNNNTVNHSV 158
           +G EF A A EL+ ++ VN  +
Sbjct: 133 YGKEFVAAASELKPDSGVNRKL 154

BLAST of Carg27339 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 45/139 (32.37%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 16  RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLM 75
           +++  + L++  +  ++ ++  R  +  + R++ A+LL  G    A  R E + R + +M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 76  DAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSR 135
            A  ++E +  L+  RL ++   RECP +L+EA+SSV FAA RC D  EL++++ +  S+
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 136 FGTEFAARAVELRNNNTVN 155
           +G EF A A EL+ ++ VN
Sbjct: 133 YGKEFVAAASELKPDSGVN 151

BLAST of Carg27339 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 44/131 (33.59%), Postives = 77/131 (58.78%), Query Frame = 0

Query: 27  ALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLN 86
           A+  ++ L+  R  +    +K+ A  L  G   +A  R E + R  NL  AY ++E +  
Sbjct: 24  AIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAAYEILELFCE 83

Query: 87  LLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFGTEFAARAVE 146
            +  R+ +L   +ECP+EL EA++S++FAA RC + P+L +IK++  +++G EF   A E
Sbjct: 84  FILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYGKEFIMVASE 143

Query: 147 LRNNNTVNHSV 158
           LR ++ VN ++
Sbjct: 144 LRPDSGVNRTI 154

BLAST of Carg27339 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 78.6 bits (192), Expect = 5.2e-15
Identity = 51/151 (33.77%), Postives = 84/151 (55.63%), Query Frame = 0

Query: 4   LRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALS 63
           L+R FKP       +  +  +Q A   L+ LK  +  +  + R++ A+LL  G    A  
Sbjct: 5   LQRGFKP-------AKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARI 64

Query: 64  RCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFP 123
           R E + R +  + AY ++  Y  LL  RL ++   + CP +L+EAV+SV+FA+ R  D P
Sbjct: 65  RVEHVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVP 124

Query: 124 ELEEIKSVLSSRFGTEFAARAVELRNNNTVN 155
           EL EI    ++++G +F+  AVELR ++ V+
Sbjct: 125 ELSEIFKQFTTKYGKDFSTSAVELRPDSGVS 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6601796.11.8e-81100.00IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032510.1... [more]
XP_022963468.11.3e-7998.74uncharacterized protein LOC111463790 [Cucurbita moschata][more]
XP_023538969.13.4e-7796.86uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo][more]
XP_023522757.11.7e-7698.08uncharacterized protein LOC111786761, partial [Cucurbita pepo subsp. pepo][more]
XP_022971780.12.4e-7594.38uncharacterized protein LOC111470458 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q54I391.5e-0627.03IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Q3ZBV18.9e-0430.83IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
P539908.9e-0430.83IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX008.9e-0430.83IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z68.9e-0430.83IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HHW46.1e-8098.74uncharacterized protein LOC111463790 OS=Cucurbita moschata OX=3662 GN=LOC1114637... [more]
A0A6J1I6P01.2e-7594.38uncharacterized protein LOC111470458 OS=Cucurbita maxima OX=3661 GN=LOC111470458... [more]
A0A6J1CEI44.9e-4564.71uncharacterized protein LOC111010490 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A1S3BD778.6e-4261.29uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=... [more]
A0A0A0K9492.8e-4059.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13340.19.4e-2542.57Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.21.5e-1731.69Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.11.9e-1732.37Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.11.9e-1733.59Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.15.2e-1533.77Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 11..159
e-value: 4.6E-37
score: 129.3
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 23..157
e-value: 4.8E-33
score: 114.4
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 13..156
NoneNo IPR availablePANTHERPTHR12161:SF16REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 13..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27339-RACarg27339-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport