Homology
BLAST of Carg27334 vs. NCBI nr
Match:
KAG7024260.1 (Trimethylguanosine synthase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1613.2 bits (4176), Expect = 0.0e+00
Identity = 787/787 (100.00%), Postives = 787/787 (100.00%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT
Sbjct: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
Query: 781 MNESNVT 788
MNESNVT
Sbjct: 781 MNESNVT 787
BLAST of Carg27334 vs. NCBI nr
Match:
KAG6591380.1 (Trimethylguanosine synthase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 784/787 (99.62%), Postives = 784/787 (99.62%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSS GLDKDHSPRICMT
Sbjct: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSNGLDKDHSPRICMT 240
Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYNKND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNKND 300
Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
NAALYGV DQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
Query: 781 MNESNVT 788
MNESNVT
Sbjct: 781 MNESNVT 787
BLAST of Carg27334 vs. NCBI nr
Match:
XP_022936355.1 (uncharacterized protein LOC111443000 isoform X2 [Cucurbita moschata])
HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 774/787 (98.35%), Postives = 778/787 (98.86%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
EVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
NSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICMT
Sbjct: 181 NSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICMT 240
Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+ND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEND 300
Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+TS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNTS 420
Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
INSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
CKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQNL 540
Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
NAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
Query: 781 MNESNVT 788
MNESNVT
Sbjct: 781 MNESNVT 787
BLAST of Carg27334 vs. NCBI nr
Match:
XP_022936353.1 (uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata] >XP_022936354.1 uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata])
HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 774/788 (98.22%), Postives = 778/788 (98.73%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120
Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240
Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300
Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420
Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540
Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
Query: 781 SMNESNVT 788
SMNESNVT
Sbjct: 781 SMNESNVT 788
BLAST of Carg27334 vs. NCBI nr
Match:
XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1563.9 bits (4048), Expect = 0.0e+00
Identity = 767/789 (97.21%), Postives = 774/789 (98.10%), Query Frame = 0
Query: 1 MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
MGS NEES EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1 MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFL 120
KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKR TTVKHSRIQQGFL
Sbjct: 61 KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQQGFL 120
Query: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
Query: 181 SPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRIC 240
SPNSSGLIHGAVEEQ CDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRIC
Sbjct: 181 SPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRIC 240
Query: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNK 300
MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+
Sbjct: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNE 300
Query: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIA 360
NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAHFDANFTENESIA
Sbjct: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTENESIA 360
Query: 361 EVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVS 420
EVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLE S ILVEGSK+RASV+
Sbjct: 361 EVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSRASVN 420
Query: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480
TSI+SYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF
Sbjct: 421 TSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480
Query: 481 SHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
SHCKPSNMY+PEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ
Sbjct: 481 SHCKPSNMYAPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
Query: 541 NLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
NLSGRDRKKKMKRTRRRGQ SD+NEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM
Sbjct: 541 NLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
Query: 601 DKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
DKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA
Sbjct: 601 DKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
Query: 661 QHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
QHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG
Sbjct: 661 QHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
Query: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780
YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN
Sbjct: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780
Query: 781 GSMNESNVT 788
GSMNESNVT
Sbjct: 781 GSMNESNVT 789
BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match:
P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)
HSP 1 Score: 258.1 bits (658), Expect = 3.3e-67
Identity = 145/334 (43.41%), Postives = 206/334 (61.68%), Query Frame = 0
Query: 452 VKQPCHENCSNGFQLIVANEASEQKTFSHCKPSNMYSPEKA---FVTIDDEGAVGLTTSS 511
V +P E S + + N+ + T S + +M S EK T D E T SS
Sbjct: 515 VNEPVDEEISQ--EPLSHNKMQDTCTSSDSEEQDM-SMEKTDGLMETRDPEPENCQTISS 574
Query: 512 VSHVLQQADHMDGDMHFGNEPTICTLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSP 571
S + +A+ +G P C+ N + + ++K+ +++ + ++N++ +
Sbjct: 575 ASEL--EAEKSEGGSLVAAVPENCSTEGVANSPRAEAEVEIKKKKKKKK-KNKNKKINGL 634
Query: 572 AITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGS----NMI 631
+ KYW QRY+LFSRFDDG+K+DKEGWFSVTPE IA H A R ++I
Sbjct: 635 PPEIASVPELAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQSFNCDII 694
Query: 632 VDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPC 691
VD+F GVGGN IQF+ K VIAIDIDP KI A++NA +YGV D+I+FI GDF LAPC
Sbjct: 695 VDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGVADKIEFICGDFLLLAPC 754
Query: 692 LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQ 751
LKADV+FLSPPWGGP+YA + +D++TM+ P DG+ +F +++KI +V FLP+N ++DQ
Sbjct: 755 LKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIFRLSQKITNNIVYFLPRNADVDQ 814
Query: 752 LAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
+A +A P +E+E+NFLN KLK ITAYF +
Sbjct: 815 VASLA---GPGGQVEIEQNFLNNKLKTITAYFGD 838
BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match:
Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)
HSP 1 Score: 251.5 bits (641), Expect = 3.1e-65
Identity = 123/236 (52.12%), Postives = 164/236 (69.49%), Query Frame = 0
Query: 547 KKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSV 606
KK K+ + + +D E S + KYW QRY+LFSRFDDG+K+DKEGWFSV
Sbjct: 612 KKKKKKNKNKKINDLPPEIAS-------VPELAKYWAQRYRLFSRFDDGIKLDKEGWFSV 671
Query: 607 TPESIARHHASRCGS----NMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNA 666
TPE IA H A R +++VD+F GVGGN IQF+ K VIAIDIDP KI A++NA
Sbjct: 672 TPEKIAEHIAGRVSQAFRCDVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNA 731
Query: 667 ALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLF 726
+YG+ D+I+FI GDF LAPCLKADV+FLSPPWGGP+YA + +D++TM+ P DG+ +F
Sbjct: 732 EVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIF 791
Query: 727 NIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
+++KI +V FLP+N ++DQ+A +A +E+E+NFLN KLK ITAYF +
Sbjct: 792 RLSQKITNNIVYFLPRNADIDQVASLAGLGG---QVEIEQNFLNNKLKTITAYFGD 836
BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match:
Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)
HSP 1 Score: 247.7 bits (631), Expect = 4.5e-64
Identity = 115/205 (56.10%), Postives = 152/205 (74.15%), Query Frame = 0
Query: 578 ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGS----NMIVDSFTGVGG 637
+ KYW QRY+LFSRFDDG+K+D+EGWFSVTPE IA H A R +++VD+F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703
Query: 638 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLS 697
N IQF+ VIAIDIDP KI A++NA +YG+ D+I+FI GDF LA LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763
Query: 698 PPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSN 757
PPWGGP+YA + +D++TM+ P DG+ +F ++KKI +V FLP+N ++DQ+A +A
Sbjct: 764 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSKKITNNIVYFLPRNADIDQVASLA---G 823
Query: 758 PPWSLEVEKNFLNGKLKAITAYFSN 779
P +E+E+NFLN KLK ITAYF +
Sbjct: 824 PGGQVEIEQNFLNNKLKTITAYFGD 844
BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match:
Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)
HSP 1 Score: 158.7 bits (400), Expect = 2.7e-37
Identity = 89/229 (38.86%), Postives = 129/229 (56.33%), Query Frame = 0
Query: 560 DRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPE----SIARHH 619
D +E I P ++ KYW RY LFSRFD+G+ +D + W+SVTPE +IA+
Sbjct: 8 DEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSV 67
Query: 620 ASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGV-GDQIDFI 679
+I+D+F+G GGN IQF++ VI+I+IDP KI A+HN +YG+ ++ FI
Sbjct: 68 VDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFI 127
Query: 680 KGDFFRLAPCLK-----ADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIA 739
+GD L+ ++F+SPPWGGP+Y+ +Y L L P+ LF A +I+
Sbjct: 128 QGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKEATRIS 187
Query: 740 PVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFL-NGKLKAITAYFS 778
P V FLP+N ++ +LA N P+ NFL G KAI YF+
Sbjct: 188 PYVAAFLPRNTDVKELAAYGSIHNKPYI----TNFLFEGYAKAICCYFN 230
BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match:
Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)
HSP 1 Score: 125.9 bits (315), Expect = 2.0e-27
Identity = 72/178 (40.45%), Postives = 98/178 (55.06%), Query Frame = 0
Query: 580 KYWCQRYQLFSRFDD-GVKMDKEGWFSVTPESIA---RHHASRCGSN--MIVDSFTGVGG 639
KYW R +LFS+ D + M E WFSVTPE IA + C N I+D F G GG
Sbjct: 51 KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110
Query: 640 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLA-----PCLKAD 699
N IQF+ + +V +D I NA YGV D+I +G + +L +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170
Query: 700 VIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLA 747
+F SPPWGGP Y R D+YDL+ L+P + K++P V+MFLP+N +L+QL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228
BLAST of Carg27334 vs. ExPASy TrEMBL
Match:
A0A6J1FDE5 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)
HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 774/787 (98.35%), Postives = 778/787 (98.86%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
EVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
NSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICMT
Sbjct: 181 NSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICMT 240
Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+ND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEND 300
Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+TS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNTS 420
Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
INSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
CKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQNL 540
Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
NAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
Query: 781 MNESNVT 788
MNESNVT
Sbjct: 781 MNESNVT 787
BLAST of Carg27334 vs. ExPASy TrEMBL
Match:
A0A6J1FD09 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)
HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 774/788 (98.22%), Postives = 778/788 (98.73%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120
Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240
Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300
Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420
Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540
Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
Query: 781 SMNESNVT 788
SMNESNVT
Sbjct: 781 SMNESNVT 788
BLAST of Carg27334 vs. ExPASy TrEMBL
Match:
A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)
HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 758/789 (96.07%), Postives = 766/789 (97.08%), Query Frame = 0
Query: 1 MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
MGS NEES EAGVSAIRA+GSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFL 120
KICSTITDISLSPEDI+LTEQMNALGLPLSFHTNKERRTGITMGKR TTVKHSRIQ GFL
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120
Query: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
Query: 181 SPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRIC 240
SPNSSGLIHGAVEEQSC+V CDIVLNNRGDHESGDA+LGDHAKVRLS IGLDK HSPRIC
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESGDALLGDHAKVRLSPIGLDKGHSPRIC 240
Query: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNK 300
MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQS+LHMGADYN+
Sbjct: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADYNE 300
Query: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIA 360
NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAH DANFTENESIA
Sbjct: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENESIA 360
Query: 361 EVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVS 420
EVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSK+RASV
Sbjct: 361 EVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRASVH 420
Query: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480
TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANE SEQKTF
Sbjct: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQKTF 480
Query: 481 SHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
SHCK SNM SPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ
Sbjct: 481 SHCKSSNMDSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
Query: 541 NLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
NLSGRDRKKKMKRTRRRGQ SDRNEEFHS AITEEYPTSITKYWCQRYQLFSRFDDGVKM
Sbjct: 541 NLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
Query: 601 DKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
DKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRYA
Sbjct: 601 DKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYA 660
Query: 661 QHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
QHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG
Sbjct: 661 QHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
Query: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780
YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS
Sbjct: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSK 780
Query: 781 GSMNESNVT 788
GSMNESNVT
Sbjct: 781 GSMNESNVT 789
BLAST of Carg27334 vs. ExPASy TrEMBL
Match:
A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)
HSP 1 Score: 1536.5 bits (3977), Expect = 0.0e+00
Identity = 758/790 (95.95%), Postives = 766/790 (96.96%), Query Frame = 0
Query: 1 MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
MGS NEES EAGVSAIRA+GSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGF 120
KICSTITDISLSPEDI+LTEQMNALGLPLSFHTNKE RRTGITMGKR TTVKHSRIQ GF
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120
Query: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180
LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180
Query: 181 ISPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRI 240
ISPNSSGLIHGAVEEQSC+V CDIVLNNRGDHESGDA+LGDHAKVRLS IGLDK HSPRI
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESGDALLGDHAKVRLSPIGLDKGHSPRI 240
Query: 241 CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYN 300
CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQS+LHMGADYN
Sbjct: 241 CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADYN 300
Query: 301 KNDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESI 360
+NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAH DANFTENESI
Sbjct: 301 ENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENESI 360
Query: 361 AEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV 420
AEVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSK+RASV
Sbjct: 361 AEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRASV 420
Query: 421 STSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKT 480
TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANE SEQKT
Sbjct: 421 HTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQKT 480
Query: 481 FSHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE 540
FSHCK SNM SPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE
Sbjct: 481 FSHCKSSNMDSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE 540
Query: 541 QNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVK 600
QNLSGRDRKKKMKRTRRRGQ SDRNEEFHS AITEEYPTSITKYWCQRYQLFSRFDDGVK
Sbjct: 541 QNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDGVK 600
Query: 601 MDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660
MDKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRY
Sbjct: 601 MDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRY 660
Query: 661 AQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD 720
AQHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD
Sbjct: 661 AQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD 720
Query: 721 GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS 780
GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS
Sbjct: 721 GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS 780
Query: 781 NGSMNESNVT 788
GSMNESNVT
Sbjct: 781 KGSMNESNVT 790
BLAST of Carg27334 vs. ExPASy TrEMBL
Match:
A0A6J1F792 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)
HSP 1 Score: 1519.2 bits (3932), Expect = 0.0e+00
Identity = 746/760 (98.16%), Postives = 750/760 (98.68%), Query Frame = 0
Query: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1 MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60
Query: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61 CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120
Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240
Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300
Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420
Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540
Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE 760
FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE 760
BLAST of Carg27334 vs. TAIR 10
Match:
AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 338.6 bits (867), Expect = 1.4e-92
Identity = 188/465 (40.43%), Postives = 255/465 (54.84%), Query Frame = 0
Query: 312 WTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEVAEMDVLEDLKP 371
W VYWDSFY R+YFYN KT ES W PPLG+EH A+ D + +E + E D+ +
Sbjct: 169 WKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSELVIEKHHDDLSGTVLG 228
Query: 372 EDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTSINSYMQPDEPH 431
+D+ D +L G CQ S + E ++ V++ I++Y +
Sbjct: 229 DDVPFDKAD-----DLGG---VCQ--------SQLEAEATE---EVNSLIDTYQETS--- 288
Query: 432 EWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSHCKPSNMYSPEK 491
+ N++ +
Sbjct: 289 ------------------------------------IGNQSLD----------------- 348
Query: 492 AFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNLSGRDRKKKMKR 551
++ +EG SSV R KK+ +R
Sbjct: 349 -ITSLGEEGTGAYVVSSV---------------------------------RKAKKESRR 408
Query: 552 TRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESI 611
+R + + + + EEY + KYWCQRY LFSRFD+G+KMD+EGWFSVTPE I
Sbjct: 409 SRAKKKLLNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPELI 468
Query: 612 ARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQI 671
A+HHA+RC +++D FTGVGGNAIQF+ R+ +VIAID+DP K+ A+HNAA+YGV D+I
Sbjct: 469 AKHHATRCNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADKI 524
Query: 672 DFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPV 731
DF+KGDFF LA LKA +FLSPPWGGP+Y + YD+KTMLRP DG LF A IA
Sbjct: 529 DFVKGDFFDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIAST 524
Query: 732 VVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYF 777
++MFLP+NV+++QLAE+ALS++PPWSLEVEKN+LNGKLKA+TAY+
Sbjct: 589 IIMFLPRNVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAYY 524
BLAST of Carg27334 vs. TAIR 10
Match:
AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 259.2 bits (661), Expect = 1.1e-68
Identity = 119/211 (56.40%), Postives = 159/211 (75.36%), Query Frame = 0
Query: 570 ITEEYPTS--ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDS 629
I +E+ T+ I++YW QRY LFS++D G++MD+EGW+SVTPE IA A RC +++D
Sbjct: 9 IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDC 68
Query: 630 FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKA 689
F+GVGGN IQF++ VIAIDIDP KI A +NA +YGV ++IDF+ GDF +LAP LK
Sbjct: 69 FSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKG 128
Query: 690 DVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE 749
DV+FLSPPWGGP Y++V+ Y L ML P DGY LF A I P ++MFLPKN++L QL E
Sbjct: 129 DVLFLSPPWGGPTYSKVESYKL-DMLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEE 188
Query: 750 MALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
+A S+PP +LE+E+N + G++KAITAYFS+
Sbjct: 189 LACLSSPPLTLEIEENSIGGEIKAITAYFSS 218
BLAST of Carg27334 vs. TAIR 10
Match:
AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 257.3 bits (656), Expect = 4.0e-68
Identity = 114/200 (57.00%), Postives = 153/200 (76.50%), Query Frame = 0
Query: 578 ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQ 637
ITKYW QRY LFSR+D G++MD+EGW+SVTPE IA A R +++D F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312
Query: 638 FSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWG 697
F++ V+AIDIDP K+ A +NA +YGV +++DF+ GDF +LAP LK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372
Query: 698 GPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWS 757
GP Y + Y+L ML+P DGY LF IA+ I P ++MFLP+NV+L Q+ E+A S+PP +
Sbjct: 373 GPMYRDFESYNL-DMLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLN 432
Query: 758 LEVEKNFLNGKLKAITAYFS 778
LE+E+NF+ G++KA+TAYFS
Sbjct: 433 LEIEENFVGGRMKAVTAYFS 451
BLAST of Carg27334 vs. TAIR 10
Match:
AT3G21300.1 (RNA methyltransferase family protein )
HSP 1 Score: 45.8 bits (107), Expect = 1.8e-04
Identity = 24/80 (30.00%), Postives = 40/80 (50.00%), Query Frame = 0
Query: 620 GSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFF 679
GS +++D F G G + ++RAKHV ++ P I A NA + G+ + FI+GD
Sbjct: 399 GSEVVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGI-ENATFIQGDLN 458
Query: 680 RLAPCL-----KADVIFLSP 695
++ K D++ P
Sbjct: 459 KIGEDFGNNFPKPDIVISDP 477
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7024260.1 | 0.0e+00 | 100.00 | Trimethylguanosine synthase [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
KAG6591380.1 | 0.0e+00 | 99.62 | Trimethylguanosine synthase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022936355.1 | 0.0e+00 | 98.35 | uncharacterized protein LOC111443000 isoform X2 [Cucurbita moschata] | [more] |
XP_022936353.1 | 0.0e+00 | 98.22 | uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata] >XP_0229363... | [more] |
XP_023535337.1 | 0.0e+00 | 97.21 | uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
P85107 | 3.3e-67 | 43.41 | Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1 | [more] |
Q923W1 | 3.1e-65 | 52.12 | Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2 | [more] |
Q96RS0 | 4.5e-64 | 56.10 | Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3 | [more] |
Q09814 | 2.7e-37 | 38.86 | Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... | [more] |
Q12052 | 2.0e-27 | 40.45 | Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FDE5 | 0.0e+00 | 98.35 | Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... | [more] |
A0A6J1FD09 | 0.0e+00 | 98.22 | Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... | [more] |
A0A6J1IFL4 | 0.0e+00 | 96.07 | Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... | [more] |
A0A6J1II65 | 0.0e+00 | 95.95 | Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... | [more] |
A0A6J1F792 | 0.0e+00 | 98.16 | Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... | [more] |
Match Name | E-value | Identity | Description | |
AT1G45231.2 | 1.4e-92 | 40.43 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
AT1G30550.2 | 1.1e-68 | 56.40 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
AT1G30550.1 | 4.0e-68 | 57.00 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
AT3G21300.1 | 1.8e-04 | 30.00 | RNA methyltransferase family protein | [more] |