Carg27334 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27334
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTrimethylguanosine synthase
LocationCarg_Chr09: 1004660 .. 1011451 (+)
RNA-Seq ExpressionCarg27334
SyntenyCarg27334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCATAGTTCAACGGGCTGTGGCTGTCGAGGCTGAAATTTCCTCTCTTACAACTTACAATATAGCTGTGTTCCGAAAGTGCGCCGCCAAATTAGCGTCGCACTGTTTTAGTAAGCTTTGAAAAATGGCGGTAGAAAGAGTGCCGTTGTAAACTGCTGATCGTAGTTCTTGGCCCGAGCTCCCTTTTCCGCCGACAAAATGGGATCTGGCAACGAAGAATCCGAAGCTGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACGGAAGTCTTTCTTTGGTAACCAATTTTTATCACCTCTGTTATCGCCGGAAGATGAGGGAAATCTAAATTAAAAGAGATTAACGACTATGTTCTTTGTTTTTGTTCAGTTCGACTTTTCTTCTTTTCCATTTCACTTGTTTGGGTATTGACAAAAATTTCTAATGGTTGCATTACAGGGACGATGAGACAGAAGTAGCTAGACGAGTGGAAAGTAGTGTAAGTCTCTGATATCACTGTTTTTATTTACGGCACTGATAATTCATTGAGTTCTCTTTGCTCCCTGGTTAATTTCCAATCTACTCATTCCTTTGTTTGTTTTAACAAGTTAGCTCTGGATGCTGATGATGCCAATGGCGAAAAGTTCAGAGAGAAAATCTGTTCTACAATCACTGGCAAGTGTTTCATTCGTCAATCTCTTCGTTTACCTAGGCTTTTATTTTATTTTAGTTATGAGATATTTGTGGCCAAATATGATGTTCACCCACTATTTCTATGGCATAATCATGCATGTTGATTGGTGCTAAAGGGTCTGCTCGATTTTTTTGGCCAGATATCAGCTTATCACCGGAGGATATCGAACTTACTGAGCAGATGAATGCTCTGGGGCTTCCTCTCTCATTCCACACAAACAAAGAGGTACTTCAATATACTACTTCTTTATATTACTCACCCAACATGTTGACACTCCTGTACTCTAGAAGTATACATTCTATTAATTAATTTCAACAGTCTTATCTTCCGTGGATGTTAAAAAACGGTTAACTGCATCGCCAAGTCAGTTTGCATCAATGTTGTGTCAATTCTACATTAATAGCAGGCTAAGGTATAATTTAACCCATTACATAAATAAGCTATTCCACATACCCTATTTTCCCCCATTCTTTTGGCCATATCCTCCTATAGTTATTGGAAATCATACAGTAACTGTGGTTGCTTTACCATACCTTTTGTTTCTTTTCTTCAAGAACAACTGCAAGTACTAATATCAGAGCCGTAAATCTATTGCATTACAAGTATGAAGTATTTAGCCTCATTTACTCTTAGCAGCTGTCGGCAGATTGATTTGTAAATGATCTGGAAATATGATTTTTCTGGAAGAAGGATAACTAAATGGATTGAGCGTTTTATACATTACCATAACCATTGGTTGGAAATAATATTTTCTCCATTTATCCATAGTCGTGATGTTTTACCATGTAGCAGAGGAGAACTGGAATTACCATGGGCAAAAGAAACACAACTGTCAAGCATTCTAGAATCCAGCAGGGATTTCTAGACAAAGAAGTGGAGTTTCCCAAATTCAGCAGCAGGGGGGAGATTGTAGCTAACATTAATTTGAATGATGAAGCAATAGGTTCTCTATGTTGCTCGTCTATGGTGAACCAAAGTGAGGCATCTGATTGTGATGCTGTGTTTGAAGCCAATGAATCCCATGTCATCTTTGATGGAGATATTTCACCTAATTCAAGTGGACTTATTCATGGTGCTGTTGAAGAACAATCTTGCGATGTTACATGTGATATTGTGCTGAATAACAGGGGAGACCATGAGTCAGGCGATGCTGTGTTGGGGGATCATGCAAAAGTTAGATTGAGTTCAATTGGTTTGGATAAAGATCATTCTCCAAGAATATGTATGACAGGTTTTGATGTCAGCCATGGCAAGCAGGAGGAAGTTGAACTGCCTATGGAGTTAGAAGGTTCATCCACGACTTTACAAGATACTGAAGTGCAGAAGATTGACATTGACAGTTGCATTGGACTGCCATTAGTAGCTGAACAATCTTTCCTTCATATGGGAGCAGACTATAACAAAAATGACCATGTTGTTGGATGTATTCAGGAGTATGGAGAATGGACGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTTTATAATATAAAGACACATGAGTCTACTTGGAACCCTCCTCTAGGGTTGGAACATTTTGCACATTTTGATGCCAATTTCACAGAAAATGAATCAATTGCCGAAGTTGCTGAAATGGATGTATTAGAAGACTTAAAACCAGAAGATATTTGTAGTGTGCTTGTTGATACCAGGTCATGTATGAATTTACCTGGTGATAATATTCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTATTTTAGTTGAGGGCTCCAAGAATAGGGCCTCTGTCAGTACTTCAATTAACAGTTATATGCAACCTGATGAACCTCATGAGTGGTTGACGAGTTGTAGAAATACCAGGGAAATTATTGAATGCAGGTTCTTGACCTCTTGTCTATGATTTCTATTTCTTACTTTTTCTCATTTACTTGTATGAGTAACTTGTTTCGAGGGGTATAATGATTATTATTGTGATTTCAGTTGTGAAGGTCATGTCAAGCAACCATGTCATGAGAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGAAGCTTCTGAACAGAAGACCTTCAGCCATTGCAAACCTAGTAACATGTACTCACCTGAGAAAGCTTTTGTCACTATAGATGACGAAGGTGCAGTGGGTTTAACTACTAGCAGGTAGCATTTGGAATCAGAATTGTAATTGATGCCTATTAGCTTCCTTATCTCACCACAAATTGCTCTTTTGTTTGGGCTCCCTTGCTGTGTTTCCAGTGTCTCTCATGTGCTACAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGAACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAGATAGGAAAAAGAAAATGAAAAGAACACGCAGACGCGGACAATTCTCTGATAGAAATGAAGGTTTATTTTTCTACCTCCATTGTACCTGCAAAATGCTTAGTTTTGTCATCAATAGACATTTTATAACGTTCCACTCCATCAAGTTTCAGCAATTAAATCTTTTTGCCAGCAGAAAGGGGAATATGGAAAGAGTTTGGGAGGAGTAAATGGACAGGACAGGTCAATTTGTTGGGTTGGTTACTTATTAAATTATGACACATACATTAAGAATCTGAAATAAAAGTATGAATGTTCGGTCTTTTTTTTCTTTTAATTATATTAGTATTTTTCTCTATGAATAACCAAACTTTCATTGAAGAAAATAAAAGAATATAAGGGCATGCGAAAAAGAAAGCTCACAAAAGTGGAGGCTACCTGAAAGTAAGGCCTCCAATCGAGCAAGATAAAAATTAAACTAATAATTACAAAAAGATCTGTAACCGAAGCCCCCAGGATACATGGAACCTAGCAAGGAGTGAAACATCAAAAGGATTAAGCTCCTTCTCTCTAAAGATTCTGTTATTCCTCTCACCTCATTGCCCCCACAAAATAGAACATACTGCTGCTTGCCATGACATGGTTCAGAAGCTCCTTCTCTCTTCATTTGCAACAATGAGATGGTTTGTGGGAGATTACATGATCGAAGAACTCTCTTGTAATCCTTATCCACGGAGACCTGGAAGTGCCACTAACCCCACTCTAGTTTTGTTCAAAAGGGGATGGACCAAATTTACACGCAATAATCTTATACCACAGAGCGCCACAACCATTTGGGCGAAGGGCCTGGTTACGGGCTCTCTAATTTCCTATACCTAAACCCCCAAAAAACTTATTTAAAGCTTTTGTCTTAGTAAGTTAATGTAGATCTGTCTGAGAGGAAACAAATAAATTAATGGGTAAATATTGTGTTTTCCCCTCCTCTTGGACAAGTCTTCATTTTGTAAAATTCGTGGTTTGTGCCTGCCTCAGGATTATGAACCTTATTTAGAGCTTTCGTATTATTCAACAATGTTAGTCCGACCAGTATGGTAGAAAATCAATTTTGTCTGGTGGTGTTTTGTGGTTGTAACTCTCAACATAATTGCTACTGCCAAATTAGTCTCTTTCAGTAGCAGATAAGATTTCTTAGTTAACCTCATGATAGAAATTGATAACGGAATGCCAAGGAATTCTTTTCTCCCTGACAAACTAATTTTCTTCTGCAGAATTTCATTCTCCTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGTCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAGTTGACTCTTTCACGGGCGTTGGAGGAAATGCCATCCAGTTTTCCCAAAGGTGATGTTTGGTTAATTTTTTCTTTGTTCATTTTCACAAGTTTACTGGGTTAGTTGACACCTTCATGTATCTTATGAAGTCAGAGAATTGGTTAGGATGTTGTCTAGTTTATTTTCCATTTGTACCAATAGTTGACAAATAAGTGGATCTGATCAGTTTGATGTGTTATTCTCTTTTAAAACCTCTAAAAAAGATCTTTAATAGAATATTCCTGACATCATCTTTGTTTAATTAACTTATTAATGATTCTACAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCATTATATGGTGTTGGAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCATGTCTCAAGGTATTGAGCCTTTTCATCTACAAAAGTTGTGATTTTTCATGTACTGGAATGAATGTTTATGTTAGAAAACAAATAATTTCCTGAGATATGTAATGAGATCTACCAAGTGTGAAATTTTATCTGTTATTTCAAAATTTTAAAATTCTCCCTTTGGCGACTAATAACCAGCATTGTGAAAGCTTGTACTTTAGATGGTGAAAGTGTCACCTGTCTGATCATTGTTAGGAGAAGGTATTACCATAAGAATACTCTGTCTGTGTGAGTGACTGAATACTGTCATAAGATTATCGATTTTTGTTACTAATAATATAATGGATGCAATGATTTGTTGGCATCATTTATTCATTATTTTCTACCGACAAGTCGGGAGTATTTTTGCAGTTTTCTTTTGAACTGTGGCTTTGATCTTGCATATTCTTTTTTTTTTTTTTTTTTTATTGATTTCTAAACTTTCAATGCTATACTTCCATCTCAGTTTTAATTATATTAAATGTTGTTGGTCTCTACCTGCTGCAGGCCGACGTTATATTCTTATCACCCCCTTGGGGAGGGCCTAATTATGCCAGAGTAGATATTTATGACCTAAAGACCATGCTCAGACCACATGATGGGTAAGCTTTCTGACTTTGATAGTTCATGCCCAGTCGATGTTGCACTATTATCTTTTTTACCATCATCTGAGCTTTGGTCTTGCCTGTACCTTTGCAGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGATGGCTCTCTCTTCAAATCCTCCGTGGTCACTTGAGGTAAGAATATTCATTTTTATTTCTCAATCTGTTGTGAATTATTGGAAGGCTTAAGAACTGACACTAATACATTGTCGATATTGTTGAAGCTGATACAGTGCTTCTAGTGGAATATGAAGAACAGGAGGGGTATTAGAATTGAATTGCCATAATAATGAATATCTAGGTTTTGCTATTCATTGTGCAATGTGCAATTTGCTGTCTGCATTAGAGCAGTAGTTTCTACTCCAAGTTAAATTTGTATTTTTCTTTATAAGGAATGCTTATGAGTCTATACATTTTCTCTCATTTACATGTAAACACATGCAATCGAAAACGAACAATTCAGGAATATGCATATAAGCTTTCATACACACTTGAATCATGAATGTGCTTGCAACCATCTGCACTCCTCTCCTAATTCGTAGAAGGTTAACTCTTTTCTTGCTCGATGCCATTTTCCCTACTTCTGATGGATAATATTTTACACCTTGATGTCGTGTAACTTTAACACAAAAATTCTTTCCAGAACTTTGTATCTTATTCACTTACTTTAAATTCTAATTATTTTTAAGTGTGTTCTCAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAGGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAAAGCAATGTCACCTAATGCTTGATGTCACATTCTCCTTTTGAGAAATTCGCCAGAAATTTTGATGGGTAGAAGGTACAAAATGAAGCTTGTTTTAGTTTATAGTTTTGCTCTACTGTTGCACTTTATTTTCTCTTGTACATTATACTAAGCTACAAGAAACCATTTAAGTTGTCGTTTTTTGCTGGCACAACAGGTGCTTAAATTATTGGCTTGACAAAACAATTTTTCGGACATGGAGAAGAGGAATAGTTCACTGTACAGTAAACAAAGCTCTTATAATTGGTGAGACCCTCTTACTTTACAAGAAAAGGATTCACAGCTATTTAGGGAAAACGTTTCAGTTTTTTGTTGTATTTATTAAATTACTGGACCCATTTAGGGGGAAAAGGAAAAAAGAAAAGAAAAAGAAAAAAACTGGGACTAATGGTAATTTAGT

mRNA sequence

CAGCATAGTTCAACGGGCTGTGGCTGTCGAGGCTGAAATTTCCTCTCTTACAACTTACAATATAGCTGTGTTCCGAAAGTGCGCCGCCAAATTAGCGTCGCACTGTTTTAGTAAGCTTTGAAAAATGGCGGTAGAAAGAGTGCCGTTGTAAACTGCTGATCGTAGTTCTTGGCCCGAGCTCCCTTTTCCGCCGACAAAATGGGATCTGGCAACGAAGAATCCGAAGCTGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACGGAAGTCTTTCTTTGGGACGATGAGACAGAAGTAGCTAGACGAGTGGAAAGTAGTTTAGCTCTGGATGCTGATGATGCCAATGGCGAAAAGTTCAGAGAGAAAATCTGTTCTACAATCACTGATATCAGCTTATCACCGGAGGATATCGAACTTACTGAGCAGATGAATGCTCTGGGGCTTCCTCTCTCATTCCACACAAACAAAGAGAGGAGAACTGGAATTACCATGGGCAAAAGAAACACAACTGTCAAGCATTCTAGAATCCAGCAGGGATTTCTAGACAAAGAAGTGGAGTTTCCCAAATTCAGCAGCAGGGGGGAGATTGTAGCTAACATTAATTTGAATGATGAAGCAATAGGTTCTCTATGTTGCTCGTCTATGGTGAACCAAAGTGAGGCATCTGATTGTGATGCTGTGTTTGAAGCCAATGAATCCCATGTCATCTTTGATGGAGATATTTCACCTAATTCAAGTGGACTTATTCATGGTGCTGTTGAAGAACAATCTTGCGATGTTACATGTGATATTGTGCTGAATAACAGGGGAGACCATGAGTCAGGCGATGCTGTGTTGGGGGATCATGCAAAAGTTAGATTGAGTTCAATTGGTTTGGATAAAGATCATTCTCCAAGAATATGTATGACAGGTTTTGATGTCAGCCATGGCAAGCAGGAGGAAGTTGAACTGCCTATGGAGTTAGAAGGTTCATCCACGACTTTACAAGATACTGAAGTGCAGAAGATTGACATTGACAGTTGCATTGGACTGCCATTAGTAGCTGAACAATCTTTCCTTCATATGGGAGCAGACTATAACAAAAATGACCATGTTGTTGGATGTATTCAGGAGTATGGAGAATGGACGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTTTATAATATAAAGACACATGAGTCTACTTGGAACCCTCCTCTAGGGTTGGAACATTTTGCACATTTTGATGCCAATTTCACAGAAAATGAATCAATTGCCGAAGTTGCTGAAATGGATGTATTAGAAGACTTAAAACCAGAAGATATTTGTAGTGTGCTTGTTGATACCAGGTCATGTATGAATTTACCTGGTGATAATATTCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTATTTTAGTTGAGGGCTCCAAGAATAGGGCCTCTGTCAGTACTTCAATTAACAGTTATATGCAACCTGATGAACCTCATGAGTGGTTGACGAGTTGTAGAAATACCAGGGAAATTATTGAATGCAGTTGTGAAGGTCATGTCAAGCAACCATGTCATGAGAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGAAGCTTCTGAACAGAAGACCTTCAGCCATTGCAAACCTAGTAACATGTACTCACCTGAGAAAGCTTTTGTCACTATAGATGACGAAGGTGCAGTGGGTTTAACTACTAGCAGTGTCTCTCATGTGCTACAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGAACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAGATAGGAAAAAGAAAATGAAAAGAACACGCAGACGCGGACAATTCTCTGATAGAAATGAAGAATTTCATTCTCCTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGTCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAGTTGACTCTTTCACGGGCGTTGGAGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCATTATATGGTGTTGGAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCATGTCTCAAGGCCGACGTTATATTCTTATCACCCCCTTGGGGAGGGCCTAATTATGCCAGAGTAGATATTTATGACCTAAAGACCATGCTCAGACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGATGGCTCTCTCTTCAAATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAGGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAAAGCAATGTCACCTAATGCTTGATGTCACATTCTCCTTTTGAGAAATTCGCCAGAAATTTTGATGGGTAGAAGGTGCTTAAATTATTGGCTTGACAAAACAATTTTTCGGACATGGAGAAGAGGAATAGTTCACTGTACAGTAAACAAAGCTCTTATAATTGGTGAGACCCTCTTACTTTACAAGAAAAGGATTCACAGCTATTTAGGGAAAACGTTTCAGTTTTTTGTTGTATTTATTAAATTACTGGACCCATTTAGGGGGAAAAGGAAAAAAGAAAAGAAAAAGAAAAAAACTGGGACTAATGGTAATTTAGT

Coding sequence (CDS)

ATGGGATCTGGCAACGAAGAATCCGAAGCTGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACGGAAGTCTTTCTTTGGGACGATGAGACAGAAGTAGCTAGACGAGTGGAAAGTAGTTTAGCTCTGGATGCTGATGATGCCAATGGCGAAAAGTTCAGAGAGAAAATCTGTTCTACAATCACTGATATCAGCTTATCACCGGAGGATATCGAACTTACTGAGCAGATGAATGCTCTGGGGCTTCCTCTCTCATTCCACACAAACAAAGAGAGGAGAACTGGAATTACCATGGGCAAAAGAAACACAACTGTCAAGCATTCTAGAATCCAGCAGGGATTTCTAGACAAAGAAGTGGAGTTTCCCAAATTCAGCAGCAGGGGGGAGATTGTAGCTAACATTAATTTGAATGATGAAGCAATAGGTTCTCTATGTTGCTCGTCTATGGTGAACCAAAGTGAGGCATCTGATTGTGATGCTGTGTTTGAAGCCAATGAATCCCATGTCATCTTTGATGGAGATATTTCACCTAATTCAAGTGGACTTATTCATGGTGCTGTTGAAGAACAATCTTGCGATGTTACATGTGATATTGTGCTGAATAACAGGGGAGACCATGAGTCAGGCGATGCTGTGTTGGGGGATCATGCAAAAGTTAGATTGAGTTCAATTGGTTTGGATAAAGATCATTCTCCAAGAATATGTATGACAGGTTTTGATGTCAGCCATGGCAAGCAGGAGGAAGTTGAACTGCCTATGGAGTTAGAAGGTTCATCCACGACTTTACAAGATACTGAAGTGCAGAAGATTGACATTGACAGTTGCATTGGACTGCCATTAGTAGCTGAACAATCTTTCCTTCATATGGGAGCAGACTATAACAAAAATGACCATGTTGTTGGATGTATTCAGGAGTATGGAGAATGGACGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTTTATAATATAAAGACACATGAGTCTACTTGGAACCCTCCTCTAGGGTTGGAACATTTTGCACATTTTGATGCCAATTTCACAGAAAATGAATCAATTGCCGAAGTTGCTGAAATGGATGTATTAGAAGACTTAAAACCAGAAGATATTTGTAGTGTGCTTGTTGATACCAGGTCATGTATGAATTTACCTGGTGATAATATTCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTATTTTAGTTGAGGGCTCCAAGAATAGGGCCTCTGTCAGTACTTCAATTAACAGTTATATGCAACCTGATGAACCTCATGAGTGGTTGACGAGTTGTAGAAATACCAGGGAAATTATTGAATGCAGTTGTGAAGGTCATGTCAAGCAACCATGTCATGAGAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGAAGCTTCTGAACAGAAGACCTTCAGCCATTGCAAACCTAGTAACATGTACTCACCTGAGAAAGCTTTTGTCACTATAGATGACGAAGGTGCAGTGGGTTTAACTACTAGCAGTGTCTCTCATGTGCTACAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGAACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAGATAGGAAAAAGAAAATGAAAAGAACACGCAGACGCGGACAATTCTCTGATAGAAATGAAGAATTTCATTCTCCTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGTCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAGTTGACTCTTTCACGGGCGTTGGAGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCATTATATGGTGTTGGAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCATGTCTCAAGGCCGACGTTATATTCTTATCACCCCCTTGGGGAGGGCCTAATTATGCCAGAGTAGATATTTATGACCTAAAGACCATGCTCAGACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGATGGCTCTCTCTTCAAATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAGGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAAAGCAATGTCACCTAA

Protein sequence

MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKNDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGSMNESNVT
Homology
BLAST of Carg27334 vs. NCBI nr
Match: KAG7024260.1 (Trimethylguanosine synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1613.2 bits (4176), Expect = 0.0e+00
Identity = 787/787 (100.00%), Postives = 787/787 (100.00%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120

Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
           EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180

Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
           NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT
Sbjct: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240

Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
           GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300

Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
           HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360

Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
           AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420

Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
           INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480

Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
           CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540

Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
           SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600

Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
           EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660

Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
           NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720

Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
           LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780

Query: 781 MNESNVT 788
           MNESNVT
Sbjct: 781 MNESNVT 787

BLAST of Carg27334 vs. NCBI nr
Match: KAG6591380.1 (Trimethylguanosine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 784/787 (99.62%), Postives = 784/787 (99.62%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120

Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
           EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180

Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
           NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSS GLDKDHSPRICMT
Sbjct: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSNGLDKDHSPRICMT 240

Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
           GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYNKND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNKND 300

Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
           HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360

Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
           AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420

Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
           INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480

Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
           CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540

Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
           SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600

Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
           EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660

Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
           NAALYGV DQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720

Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
           LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780

Query: 781 MNESNVT 788
           MNESNVT
Sbjct: 781 MNESNVT 787

BLAST of Carg27334 vs. NCBI nr
Match: XP_022936355.1 (uncharacterized protein LOC111443000 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 774/787 (98.35%), Postives = 778/787 (98.86%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120

Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
           EVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180

Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
           NSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICMT
Sbjct: 181 NSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICMT 240

Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
           GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+ND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEND 300

Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
           HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360

Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
           AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+TS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNTS 420

Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
           INSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480

Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
           CKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQNL 540

Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
           SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600

Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
           EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660

Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
           NAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720

Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
           LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780

Query: 781 MNESNVT 788
           MNESNVT
Sbjct: 781 MNESNVT 787

BLAST of Carg27334 vs. NCBI nr
Match: XP_022936353.1 (uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata] >XP_022936354.1 uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 774/788 (98.22%), Postives = 778/788 (98.73%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120

Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
           KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180

Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
           PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240

Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
           TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300

Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
           DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360

Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
           VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420

Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
           SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480

Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
           HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540

Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
           LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600

Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
           KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660

Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
           HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720

Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
           FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780

Query: 781 SMNESNVT 788
           SMNESNVT
Sbjct: 781 SMNESNVT 788

BLAST of Carg27334 vs. NCBI nr
Match: XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1563.9 bits (4048), Expect = 0.0e+00
Identity = 767/789 (97.21%), Postives = 774/789 (98.10%), Query Frame = 0

Query: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
           MGS NEES  EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFL 120
           KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKR TTVKHSRIQQGFL
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQQGFL 120

Query: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
           DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRIC 240
           SPNSSGLIHGAVEEQ CDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRIC
Sbjct: 181 SPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRIC 240

Query: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNK 300
           MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+
Sbjct: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNE 300

Query: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIA 360
           NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAHFDANFTENESIA
Sbjct: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTENESIA 360

Query: 361 EVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVS 420
           EVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLE S ILVEGSK+RASV+
Sbjct: 361 EVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSRASVN 420

Query: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480
           TSI+SYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF
Sbjct: 421 TSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480

Query: 481 SHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
           SHCKPSNMY+PEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ
Sbjct: 481 SHCKPSNMYAPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540

Query: 541 NLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
           NLSGRDRKKKMKRTRRRGQ SD+NEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM
Sbjct: 541 NLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600

Query: 601 DKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
           DKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA
Sbjct: 601 DKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660

Query: 661 QHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
           QHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG
Sbjct: 661 QHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720

Query: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780
           YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN
Sbjct: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780

Query: 781 GSMNESNVT 788
           GSMNESNVT
Sbjct: 781 GSMNESNVT 789

BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match: P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.3e-67
Identity = 145/334 (43.41%), Postives = 206/334 (61.68%), Query Frame = 0

Query: 452 VKQPCHENCSNGFQLIVANEASEQKTFSHCKPSNMYSPEKA---FVTIDDEGAVGLTTSS 511
           V +P  E  S   + +  N+  +  T S  +  +M S EK      T D E     T SS
Sbjct: 515 VNEPVDEEISQ--EPLSHNKMQDTCTSSDSEEQDM-SMEKTDGLMETRDPEPENCQTISS 574

Query: 512 VSHVLQQADHMDGDMHFGNEPTICTLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSP 571
            S +  +A+  +G       P  C+     N    + + ++K+ +++ +  ++N++ +  
Sbjct: 575 ASEL--EAEKSEGGSLVAAVPENCSTEGVANSPRAEAEVEIKKKKKKKK-KNKNKKINGL 634

Query: 572 AITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGS----NMI 631
                    + KYW QRY+LFSRFDDG+K+DKEGWFSVTPE IA H A R       ++I
Sbjct: 635 PPEIASVPELAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQSFNCDII 694

Query: 632 VDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPC 691
           VD+F GVGGN IQF+   K VIAIDIDP KI  A++NA +YGV D+I+FI GDF  LAPC
Sbjct: 695 VDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGVADKIEFICGDFLLLAPC 754

Query: 692 LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQ 751
           LKADV+FLSPPWGGP+YA  + +D++TM+ P DG+ +F +++KI   +V FLP+N ++DQ
Sbjct: 755 LKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIFRLSQKITNNIVYFLPRNADVDQ 814

Query: 752 LAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
           +A +A    P   +E+E+NFLN KLK ITAYF +
Sbjct: 815 VASLA---GPGGQVEIEQNFLNNKLKTITAYFGD 838

BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match: Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 3.1e-65
Identity = 123/236 (52.12%), Postives = 164/236 (69.49%), Query Frame = 0

Query: 547 KKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSV 606
           KK K+  +  + +D   E  S          + KYW QRY+LFSRFDDG+K+DKEGWFSV
Sbjct: 612 KKKKKKNKNKKINDLPPEIAS-------VPELAKYWAQRYRLFSRFDDGIKLDKEGWFSV 671

Query: 607 TPESIARHHASRCGS----NMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNA 666
           TPE IA H A R       +++VD+F GVGGN IQF+   K VIAIDIDP KI  A++NA
Sbjct: 672 TPEKIAEHIAGRVSQAFRCDVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNA 731

Query: 667 ALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLF 726
            +YG+ D+I+FI GDF  LAPCLKADV+FLSPPWGGP+YA  + +D++TM+ P DG+ +F
Sbjct: 732 EVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIF 791

Query: 727 NIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
            +++KI   +V FLP+N ++DQ+A +A        +E+E+NFLN KLK ITAYF +
Sbjct: 792 RLSQKITNNIVYFLPRNADIDQVASLAGLGG---QVEIEQNFLNNKLKTITAYFGD 836

BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match: Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)

HSP 1 Score: 247.7 bits (631), Expect = 4.5e-64
Identity = 115/205 (56.10%), Postives = 152/205 (74.15%), Query Frame = 0

Query: 578 ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGS----NMIVDSFTGVGG 637
           + KYW QRY+LFSRFDDG+K+D+EGWFSVTPE IA H A R       +++VD+F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703

Query: 638 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLS 697
           N IQF+     VIAIDIDP KI  A++NA +YG+ D+I+FI GDF  LA  LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763

Query: 698 PPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSN 757
           PPWGGP+YA  + +D++TM+ P DG+ +F ++KKI   +V FLP+N ++DQ+A +A    
Sbjct: 764 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSKKITNNIVYFLPRNADIDQVASLA---G 823

Query: 758 PPWSLEVEKNFLNGKLKAITAYFSN 779
           P   +E+E+NFLN KLK ITAYF +
Sbjct: 824 PGGQVEIEQNFLNNKLKTITAYFGD 844

BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match: Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)

HSP 1 Score: 158.7 bits (400), Expect = 2.7e-37
Identity = 89/229 (38.86%), Postives = 129/229 (56.33%), Query Frame = 0

Query: 560 DRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPE----SIARHH 619
           D +E      I    P ++ KYW  RY LFSRFD+G+ +D + W+SVTPE    +IA+  
Sbjct: 8   DEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSV 67

Query: 620 ASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGV-GDQIDFI 679
                  +I+D+F+G GGN IQF++    VI+I+IDP KI  A+HN  +YG+   ++ FI
Sbjct: 68  VDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFI 127

Query: 680 KGDFFRLAPCLK-----ADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIA 739
           +GD       L+       ++F+SPPWGGP+Y+   +Y L   L P+    LF  A +I+
Sbjct: 128 QGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKEATRIS 187

Query: 740 PVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFL-NGKLKAITAYFS 778
           P V  FLP+N ++ +LA      N P+      NFL  G  KAI  YF+
Sbjct: 188 PYVAAFLPRNTDVKELAAYGSIHNKPYI----TNFLFEGYAKAICCYFN 230

BLAST of Carg27334 vs. ExPASy Swiss-Prot
Match: Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 2.0e-27
Identity = 72/178 (40.45%), Postives = 98/178 (55.06%), Query Frame = 0

Query: 580 KYWCQRYQLFSRFDD-GVKMDKEGWFSVTPESIA---RHHASRCGSN--MIVDSFTGVGG 639
           KYW  R +LFS+ D   + M  E WFSVTPE IA    +    C  N   I+D F G GG
Sbjct: 51  KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110

Query: 640 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLA-----PCLKAD 699
           N IQF+ +  +V  +D     I     NA  YGV D+I   +G + +L        +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170

Query: 700 VIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLA 747
            +F SPPWGGP Y R D+YDL+  L+P     +     K++P V+MFLP+N +L+QL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228

BLAST of Carg27334 vs. ExPASy TrEMBL
Match: A0A6J1FDE5 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 774/787 (98.35%), Postives = 778/787 (98.86%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDK 120

Query: 121 EVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180
           EVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP
Sbjct: 121 EVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISP 180

Query: 181 NSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICMT 240
           NSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICMT
Sbjct: 181 NSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICMT 240

Query: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKND 300
           GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+ND
Sbjct: 241 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEND 300

Query: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360
           HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV
Sbjct: 301 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 360

Query: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTS 420
           AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+TS
Sbjct: 361 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNTS 420

Query: 421 INSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480
           INSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH
Sbjct: 421 INSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 480

Query: 481 CKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNL 540
           CKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQNL
Sbjct: 481 CKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQNL 540

Query: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600
           SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK
Sbjct: 541 SGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDK 600

Query: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660
           EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH
Sbjct: 601 EGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQH 660

Query: 661 NAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720
           NAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF
Sbjct: 661 NAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYF 720

Query: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780
           LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS
Sbjct: 721 LFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNGS 780

Query: 781 MNESNVT 788
           MNESNVT
Sbjct: 781 MNESNVT 787

BLAST of Carg27334 vs. ExPASy TrEMBL
Match: A0A6J1FD09 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 774/788 (98.22%), Postives = 778/788 (98.73%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120

Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
           KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180

Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
           PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240

Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
           TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300

Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
           DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360

Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
           VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420

Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
           SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480

Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
           HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540

Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
           LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600

Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
           KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660

Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
           HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720

Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780
           FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSNG 780

Query: 781 SMNESNVT 788
           SMNESNVT
Sbjct: 781 SMNESNVT 788

BLAST of Carg27334 vs. ExPASy TrEMBL
Match: A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 758/789 (96.07%), Postives = 766/789 (97.08%), Query Frame = 0

Query: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
           MGS NEES  EAGVSAIRA+GSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFL 120
           KICSTITDISLSPEDI+LTEQMNALGLPLSFHTNKERRTGITMGKR TTVKHSRIQ GFL
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120

Query: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
           DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRIC 240
           SPNSSGLIHGAVEEQSC+V CDIVLNNRGDHESGDA+LGDHAKVRLS IGLDK HSPRIC
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESGDALLGDHAKVRLSPIGLDKGHSPRIC 240

Query: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNK 300
           MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQS+LHMGADYN+
Sbjct: 241 MTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADYNE 300

Query: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIA 360
           NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAH DANFTENESIA
Sbjct: 301 NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENESIA 360

Query: 361 EVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVS 420
           EVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSK+RASV 
Sbjct: 361 EVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRASVH 420

Query: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTF 480
           TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANE SEQKTF
Sbjct: 421 TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQKTF 480

Query: 481 SHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540
           SHCK SNM SPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ
Sbjct: 481 SHCKSSNMDSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQ 540

Query: 541 NLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600
           NLSGRDRKKKMKRTRRRGQ SDRNEEFHS AITEEYPTSITKYWCQRYQLFSRFDDGVKM
Sbjct: 541 NLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDGVKM 600

Query: 601 DKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
           DKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRYA
Sbjct: 601 DKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYA 660

Query: 661 QHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720
           QHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG
Sbjct: 661 QHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDG 720

Query: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSN 780
           YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS 
Sbjct: 721 YFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFSK 780

Query: 781 GSMNESNVT 788
           GSMNESNVT
Sbjct: 781 GSMNESNVT 789

BLAST of Carg27334 vs. ExPASy TrEMBL
Match: A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1536.5 bits (3977), Expect = 0.0e+00
Identity = 758/790 (95.95%), Postives = 766/790 (96.96%), Query Frame = 0

Query: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFRE 60
           MGS NEES  EAGVSAIRA+GSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFRE
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGF 120
           KICSTITDISLSPEDI+LTEQMNALGLPLSFHTNKE RRTGITMGKR TTVKHSRIQ GF
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180
           LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRI 240
           ISPNSSGLIHGAVEEQSC+V CDIVLNNRGDHESGDA+LGDHAKVRLS IGLDK HSPRI
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHESGDALLGDHAKVRLSPIGLDKGHSPRI 240

Query: 241 CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYN 300
           CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQS+LHMGADYN
Sbjct: 241 CMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADYN 300

Query: 301 KNDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESI 360
           +NDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPP GLEHFAH DANFTENESI
Sbjct: 301 ENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENESI 360

Query: 361 AEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV 420
           AEVAEMDVLED KPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSK+RASV
Sbjct: 361 AEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRASV 420

Query: 421 STSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKT 480
            TSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANE SEQKT
Sbjct: 421 HTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQKT 480

Query: 481 FSHCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE 540
           FSHCK SNM SPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE
Sbjct: 481 FSHCKSSNMDSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTE 540

Query: 541 QNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVK 600
           QNLSGRDRKKKMKRTRRRGQ SDRNEEFHS AITEEYPTSITKYWCQRYQLFSRFDDGVK
Sbjct: 541 QNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDGVK 600

Query: 601 MDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660
           MDKEGWFSVTPESIARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP KIRY
Sbjct: 601 MDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRY 660

Query: 661 AQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD 720
           AQHNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD
Sbjct: 661 AQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHD 720

Query: 721 GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS 780
           GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS
Sbjct: 721 GYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYFS 780

Query: 781 NGSMNESNVT 788
            GSMNESNVT
Sbjct: 781 KGSMNESNVT 790

BLAST of Carg27334 vs. ExPASy TrEMBL
Match: A0A6J1F792 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1519.2 bits (3932), Expect = 0.0e+00
Identity = 746/760 (98.16%), Postives = 750/760 (98.68%), Query Frame = 0

Query: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANGEKFREKI 60
           MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDAN EKFREKI
Sbjct: 1   MGSGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKI 60

Query: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE-RRTGITMGKRNTTVKHSRIQQGFLD 120
           CSTITDISLSPEDIELTEQMNALGLPLSFHTNKE RRTGITMGKRNTTVKHSRIQQGFLD
Sbjct: 61  CSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGFLD 120

Query: 121 KEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180
           KEVEFPK SSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS
Sbjct: 121 KEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDIS 180

Query: 181 PNSSGLIHGAVEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKDHSPRICM 240
           PNSSGLIHGA EEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDK HSPRICM
Sbjct: 181 PNSSGLIHGAFEEQSCDVTCDIVLNNRGDHESGDAVLGDHAKVRLSSIGLDKGHSPRICM 240

Query: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSCIGLPLVAEQSFLHMGADYNKN 300
           TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDS IGLPLVAEQSFLHMGADYN+N
Sbjct: 241 TGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEN 300

Query: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360
           DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE
Sbjct: 301 DHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAE 360

Query: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVST 420
           VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASV+T
Sbjct: 361 VAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNT 420

Query: 421 SINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480
           SINSYMQPDEPHEWLT+ RNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS
Sbjct: 421 SINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFS 480

Query: 481 HCKPSNMYSPEKAFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQN 540
           HCKPSNMYSPEKAFVTIDDEGAV LTTSSVSHVLQQADHM+GDMHFGNEPTICTLGTEQN
Sbjct: 481 HCKPSNMYSPEKAFVTIDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 540

Query: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600
           LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD
Sbjct: 541 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 600

Query: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660
           KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ
Sbjct: 601 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 660

Query: 661 HNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720
           HNAALYGV DQIDFIKGDFFRLAP LKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY
Sbjct: 661 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 720

Query: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE 760
           FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE
Sbjct: 721 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE 760

BLAST of Carg27334 vs. TAIR 10
Match: AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 1.4e-92
Identity = 188/465 (40.43%), Postives = 255/465 (54.84%), Query Frame = 0

Query: 312 WTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEVAEMDVLEDLKP 371
           W VYWDSFY R+YFYN KT ES W PPLG+EH A+ D +   +E + E    D+   +  
Sbjct: 169 WKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSELVIEKHHDDLSGTVLG 228

Query: 372 EDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVSTSINSYMQPDEPH 431
           +D+     D     +L G    CQ        S +  E ++    V++ I++Y +     
Sbjct: 229 DDVPFDKAD-----DLGG---VCQ--------SQLEAEATE---EVNSLIDTYQETS--- 288

Query: 432 EWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSHCKPSNMYSPEK 491
                                               + N++ +                 
Sbjct: 289 ------------------------------------IGNQSLD----------------- 348

Query: 492 AFVTIDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLGTEQNLSGRDRKKKMKR 551
              ++ +EG      SSV                                 R  KK+ +R
Sbjct: 349 -ITSLGEEGTGAYVVSSV---------------------------------RKAKKESRR 408

Query: 552 TRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESI 611
           +R + +  +         + EEY   + KYWCQRY LFSRFD+G+KMD+EGWFSVTPE I
Sbjct: 409 SRAKKKLLNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPELI 468

Query: 612 ARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQI 671
           A+HHA+RC   +++D FTGVGGNAIQF+ R+ +VIAID+DP K+  A+HNAA+YGV D+I
Sbjct: 469 AKHHATRCNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADKI 524

Query: 672 DFIKGDFFRLAPCLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPV 731
           DF+KGDFF LA  LKA  +FLSPPWGGP+Y +   YD+KTMLRP DG  LF  A  IA  
Sbjct: 529 DFVKGDFFDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIAST 524

Query: 732 VVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAITAYF 777
           ++MFLP+NV+++QLAE+ALS++PPWSLEVEKN+LNGKLKA+TAY+
Sbjct: 589 IIMFLPRNVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAYY 524

BLAST of Carg27334 vs. TAIR 10
Match: AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 1.1e-68
Identity = 119/211 (56.40%), Postives = 159/211 (75.36%), Query Frame = 0

Query: 570 ITEEYPTS--ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDS 629
           I +E+ T+  I++YW QRY LFS++D G++MD+EGW+SVTPE IA   A RC   +++D 
Sbjct: 9   IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDC 68

Query: 630 FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKA 689
           F+GVGGN IQF++    VIAIDIDP KI  A +NA +YGV ++IDF+ GDF +LAP LK 
Sbjct: 69  FSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKG 128

Query: 690 DVIFLSPPWGGPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE 749
           DV+FLSPPWGGP Y++V+ Y L  ML P DGY LF  A  I P ++MFLPKN++L QL E
Sbjct: 129 DVLFLSPPWGGPTYSKVESYKL-DMLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEE 188

Query: 750 MALSSNPPWSLEVEKNFLNGKLKAITAYFSN 779
           +A  S+PP +LE+E+N + G++KAITAYFS+
Sbjct: 189 LACLSSPPLTLEIEENSIGGEIKAITAYFSS 218

BLAST of Carg27334 vs. TAIR 10
Match: AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 4.0e-68
Identity = 114/200 (57.00%), Postives = 153/200 (76.50%), Query Frame = 0

Query: 578 ITKYWCQRYQLFSRFDDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQ 637
           ITKYW QRY LFSR+D G++MD+EGW+SVTPE IA   A R    +++D F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312

Query: 638 FSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFFRLAPCLKADVIFLSPPWG 697
           F++    V+AIDIDP K+  A +NA +YGV +++DF+ GDF +LAP LK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372

Query: 698 GPNYARVDIYDLKTMLRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWS 757
           GP Y   + Y+L  ML+P DGY LF IA+ I P ++MFLP+NV+L Q+ E+A  S+PP +
Sbjct: 373 GPMYRDFESYNL-DMLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLN 432

Query: 758 LEVEKNFLNGKLKAITAYFS 778
           LE+E+NF+ G++KA+TAYFS
Sbjct: 433 LEIEENFVGGRMKAVTAYFS 451

BLAST of Carg27334 vs. TAIR 10
Match: AT3G21300.1 (RNA methyltransferase family protein )

HSP 1 Score: 45.8 bits (107), Expect = 1.8e-04
Identity = 24/80 (30.00%), Postives = 40/80 (50.00%), Query Frame = 0

Query: 620 GSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAALYGVGDQIDFIKGDFF 679
           GS +++D F G G   +  ++RAKHV   ++ P  I  A  NA + G+ +   FI+GD  
Sbjct: 399 GSEVVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGI-ENATFIQGDLN 458

Query: 680 RLAPCL-----KADVIFLSP 695
           ++         K D++   P
Sbjct: 459 KIGEDFGNNFPKPDIVISDP 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024260.10.0e+00100.00Trimethylguanosine synthase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6591380.10.0e+0099.62Trimethylguanosine synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022936355.10.0e+0098.35uncharacterized protein LOC111443000 isoform X2 [Cucurbita moschata][more]
XP_022936353.10.0e+0098.22uncharacterized protein LOC111443000 isoform X1 [Cucurbita moschata] >XP_0229363... [more]
XP_023535337.10.0e+0097.21uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P851073.3e-6743.41Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1[more]
Q923W13.1e-6552.12Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2[more]
Q96RS04.5e-6456.10Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3[more]
Q098142.7e-3738.86Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Q120522.0e-2740.45Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A6J1FDE50.0e+0098.35Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
A0A6J1FD090.0e+0098.22Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
A0A6J1IFL40.0e+0096.07Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1II650.0e+0095.95Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1F7920.0e+0098.16Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G45231.21.4e-9240.43S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.21.1e-6856.40S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.14.0e-6857.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G21300.11.8e-0430.00RNA methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.20.70.10coord: 305..358
e-value: 7.6E-6
score: 27.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 571..784
e-value: 9.9E-80
score: 269.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 542..562
NoneNo IPR availablePANTHERPTHR14741S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATEDcoord: 22..783
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 624..722
e-value: 2.36922E-9
score: 53.5879
IPR019012RNA cap guanine-N2 methyltransferasePFAMPF09445Methyltransf_15coord: 622..777
e-value: 1.5E-39
score: 135.2
IPR001202WW domainPROSITEPS01159WW_DOMAIN_1coord: 312..338
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 312..340
score: 10.3443
IPR001202WW domainCDDcd00201WWcoord: 312..338
e-value: 5.25606E-4
score: 35.9666
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 594..724

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27334-RACarg27334-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009452 7-methylguanosine RNA capping
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0005515 protein binding