Carg27300 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27300
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	50S ribosomal protein L18-like
Location	Carg_Chr15: 28669 .. 30432 (-)
RNA-Seq Expression	Carg27300
Synteny	Carg27300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCACCGCAATATCTTCTTCACTGCAGTCTTCTTCTTCTTCTTCTGTGCCTTCAGTTATCTCCTTCCAATGCCCGTCATCTCCCGCCATTCTATCACAGAAATCCTCGTCTCTCTCGTGGGCATCTTCATTTCCGCATGTTACCATATCCAGAGGCTCTCCTGTCGTCTCTTCGAATCCCGCGCATGACAAGGTATAATTTCTTTACTGCCGTCCTATTCGATTCCTTGTCTGTTCCGGAGGATATCATACCTTCATTTTGAAGTTTATGCATTATCGATTGCGGACCTCTTGCGCAACATCCACGAATTTCTCGATAGGGAGAATTATTTGCAAGGAAGTTTAATATGTTGTGGGTTTGCTGCTTGTTGTGTATTCGAGCCAATAGTCAGTACGAACTCGACCATATCTCAACTTGCCTAAAACTGCTGCATAGTAAAGTAGAGTCTTCTTCAGGATATGGTTTTGAGAATTTACTGCCTGCAGTCATTTTTAGGAGAATAGTCGTGCTGTTATTATGGCTGGACAACACAGAAATGACTGTCACATGGTATACAGAGTTCTCAACTATTTTCTGCTTCGTTCCAGGTCATATGACTTTATTACTATTGTTGAGCCATATTCCAGTTCCGCATCTTTATAATTCTTCGTTTTCGACCTATTTTATTGTTTCAAAAGCTTGTTGCTAGCAGGCAAGCTTGGATTCAGGTCTAAAACTCGTGCTGTGGCCTGTGATTACGAGACTCACTAGCTACATGGTTAATTAGGACTTGTGCCGATCTTCAACCTTTTGATTTTCGCCTCTCTGAAAATTTCAATGCAGGGGGAAGTGGTTGGTTTTTTGGTCAGTTTTTTTTTTTTAACCAAATACTCGTTTGGGCTCCATAGACTTGGACCCCACATTAGATAAACATGGGTTCCATCTCAAAACTGATTTGACAATGAGATGAGTAACTCATGTATTGTTTAAAGATGGTGAGATCACCCATCTTTCCAATGTGGGATCTTCAACACTTATTGACGTACCAATTTGTCAAACACATAGATACATGCATGCCTAATAAAGGGGGTGTTGATTGGAAGATAATCTGAAACATAGCGTAGTTTTCCGTCCAAGTTTTTATAAACTTTTAATTGCAGGTGTTCTTATTATCCCGTGCATTTTTGCGTTATTTTAGAGTAGGAAGCCAATGTATATATTTTTCATTGCTGCAATCGGCTTGACGCTGTATACTGTGAGGCCTACATTAGAGCAAGGCCCGCTTAAAGCTGCATTTAGTTGATAAATGCAATGTATTCACTCTTTTAACAGATCCAGGTTACTGTTACGATTTGAATTTCTTTTGATAATGAATCAGCAAGTTTACACTTGTAGGTCTTATTTATACAATCAGCTTGGACAAGAAGGTCACGCGAGGAGGCTGCAAAACGACCGAACAAGAAATCATGGAAACAAAGGACAGATATGTATATGAAGCCATTTTTACTCGACGTTTTCTTTTCGAAGAAATTTGTTCATGCAAAGGTAATGCATAGAGGAACAAGCAAAGTGATATCGGTTGCGACAACAAATGCAAAGGATCTTAGGAACAGTCTACCATCACTTACAGATCACAATGCATGCAGAGTAATAGGGAAGCTTATTGCCGAGAGATCAAAGGAAGCAGATGTCTATGCAATGTCGTACGAGCCTAGGAAGGATGAAAGAATTGACGGTAAGCTTGGCATTGTTATTGATACCATTAAGGAGAATGGGATA

mRNA sequence

ATGGCCTTCACCGCAATATCTTCTTCACTGCAGTCTTCTTCTTCTTCTTCTGTGCCTTCAGTTATCTCCTTCCAATGCCCGTCATCTCCCGCCATTCTATCACAGAAATCCTCGTCTCTCTCGTGGGCATCTTCATTTCCGCATGTTACCATATCCAGAGGCTCTCCTGTCGTCTCTTCGAATCCCGCGCATGACAAGGTCTTATTTATACAATCAGCTTGGACAAGAAGGTCACGCGAGGAGGCTGCAAAACGACCGAACAAGAAATCATGGAAACAAAGGACAGATATGTATATGAAGCCATTTTTACTCGACGTTTTCTTTTCGAAGAAATTTGTTCATGCAAAGGTAATGCATAGAGGAACAAGCAAAGTGATATCGGTTGCGACAACAAATGCAAAGGATCTTAGGAACAGTCTACCATCACTTACAGATCACAATGCATGCAGAGTAATAGGGAAGCTTATTGCCGAGAGATCAAAGGAAGCAGATGTCTATGCAATGTCGTACGAGCCTAGGAAGGATGAAAGAATTGACGGTAAGCTTGGCATTGTTATTGATACCATTAAGGAGAATGGGATA

Coding sequence (CDS)

ATGGCCTTCACCGCAATATCTTCTTCACTGCAGTCTTCTTCTTCTTCTTCTGTGCCTTCAGTTATCTCCTTCCAATGCCCGTCATCTCCCGCCATTCTATCACAGAAATCCTCGTCTCTCTCGTGGGCATCTTCATTTCCGCATGTTACCATATCCAGAGGCTCTCCTGTCGTCTCTTCGAATCCCGCGCATGACAAGGTCTTATTTATACAATCAGCTTGGACAAGAAGGTCACGCGAGGAGGCTGCAAAACGACCGAACAAGAAATCATGGAAACAAAGGACAGATATGTATATGAAGCCATTTTTACTCGACGTTTTCTTTTCGAAGAAATTTGTTCATGCAAAGGTAATGCATAGAGGAACAAGCAAAGTGATATCGGTTGCGACAACAAATGCAAAGGATCTTAGGAACAGTCTACCATCACTTACAGATCACAATGCATGCAGAGTAATAGGGAAGCTTATTGCCGAGAGATCAAAGGAAGCAGATGTCTATGCAATGTCGTACGAGCCTAGGAAGGATGAAAGAATTGACGGTAAGCTTGGCATTGTTATTGATACCATTAAGGAGAATGGGATA

Protein sequence

MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSSNPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI

Homology

[Close]

BLAST of Carg27300 vs. NCBI nr
Match: KAG7015874.1 (rplR, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 368.6 bits (945), Expect = 3.3e-98
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS
Sbjct: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 194

[Close]

BLAST of Carg27300 vs. NCBI nr
Match: XP_022939331.1 (uncharacterized protein LOC111445279 [Cucurbita moschata])

HSP 1 Score: 368.6 bits (945), Expect = 3.3e-98
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS
Sbjct: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 194

[Close]

BLAST of Carg27300 vs. NCBI nr
Match: XP_023550056.1 (uncharacterized protein LOC111808358 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.3 bits (939), Expect = 1.6e-97
Identity = 193/194 (99.48%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSSL SSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS
Sbjct: 1   MAFTAISSSLPSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 194

[Close]

BLAST of Carg27300 vs. NCBI nr
Match: XP_022993255.1 (uncharacterized protein LOC111489327 [Cucurbita maxima])

HSP 1 Score: 339.0 bits (868), Expect = 2.8e-89
Identity = 181/194 (93.30%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAF AISSSL   S SSVPSV+SFQ PS+P ILSQKSSSLSWASSFPHVTISRGS VVSS
Sbjct: 1   MAFIAISSSL--PSCSSVPSVLSFQWPSTPTILSQKSSSLSWASSFPHVTISRGSHVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFI+SAWTRRSREEAAKRPNKKSWKQRTDMYM+PFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIRSAWTRRSREEAAKRPNKKSWKQRTDMYMRPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERI+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 192

[Close]

BLAST of Carg27300 vs. NCBI nr
Match: XP_023521951.1 (uncharacterized protein LOC111785799 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318.9 bits (816), Expect = 3.0e-83
Identity = 169/194 (87.11%), Postives = 181/194 (93.30%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSS+   SS+SV S++S QC S+P   SQKSSSLSWASSFP +++SRGSP VSS
Sbjct: 1   MAFTAISSSM--PSSASVRSILSSQCISTPTTPSQKSSSLSWASSFPSISLSRGSPFVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAH KVLFIQSAWTRRSR EAAKRPNKKSWKQRTDMYM+PFLL+VFFSKKF+HAKVMHR
Sbjct: 61  NPAHAKVLFIQSAWTRRSRGEAAKRPNKKSWKQRTDMYMRPFLLNVFFSKKFIHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERI+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 192

[Close]

BLAST of Carg27300 vs. ExPASy Swiss-Prot
Match: Q5P316 (50S ribosomal protein L18 OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=rplR PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-06
Identity = 37/119 (31.09%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 76  RRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKD 135
           RR+R+  AK    KS +           L VF +   ++A+V+    S+V++ A+T   D
Sbjct: 9   RRARKTRAKIAELKSVR-----------LTVFRTNSHIYAQVIDGSGSRVLAAASTVEAD 68

Query: 136 LRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
           +R+ LP+  +  A +V+GKLIAER+K A V A++++ R   +  G++  + +  +E G+
Sbjct: 69  VRSQLPNGGNKQAAQVVGKLIAERAKAAGVEAVAFD-RAGFQYHGRVKALAEAAREGGL 115

[Close]

BLAST of Carg27300 vs. ExPASy Swiss-Prot
Match: B7L0T4 (50S ribosomal protein L18 OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688) OX=440085 GN=rplR PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-05
Identity = 28/91 (30.77%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 104 LDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEA 163
           L VF S K ++ +V+     + ++ A++  KDL++SL +  D  A   +GKL+AER+K A
Sbjct: 29  LSVFRSSKQIYVQVIDDAAGRTLAAASSLDKDLKSSLKTGADKAAAEAVGKLVAERAKAA 88

Query: 164 DVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
            V  + ++ R      G++  + D  +E G+
Sbjct: 89  GVTKVVFD-RSGYIFHGRVKALADAAREGGL 118

[Close]

BLAST of Carg27300 vs. ExPASy Swiss-Prot
Match: A9W4S4 (50S ribosomal protein L18 OS=Methylorubrum extorquens (strain PA1) OX=419610 GN=rplR PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-05
Identity = 28/91 (30.77%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 104 LDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEA 163
           L VF S K ++ +V+     + ++ A++  KDL++SL +  D  A   +GKL+AER+K A
Sbjct: 29  LSVFRSSKQIYVQVIDDAAGRTLAAASSLDKDLKSSLKTGADKAAAEAVGKLVAERAKAA 88

Query: 164 DVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
            V  + ++ R      G++  + D  +E G+
Sbjct: 89  GVTKVVFD-RSGYIFHGRVKALADAAREGGL 118

[Close]

BLAST of Carg27300 vs. ExPASy Swiss-Prot
Match: A9BG02 (50S ribosomal protein L18 OS=Petrotoga mobilis (strain DSM 10674 / SJ95) OX=403833 GN=rplR PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-05
Identity = 31/119 (26.05%), Postives = 64/119 (53.78%), Query Frame = 0


Query: 84  KRPNKKSWKQRTDMYMKPFL--------LDVFFSKKFVHAKVMHRGTSKVISVATTNAKD 143
           K+ +KK+ +Q+  + ++  +        L VF S+K ++A+++       ++ A+T  K 
Sbjct: 3   KQLDKKALRQKRHLRVRKNVRGTSEKPRLTVFKSQKHIYAQIIDDTKGVTLASASTTQKQ 62

Query: 144 LRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
           L+  L    D NA + +GKLIAE++KE  +  + ++ R   +  GK+  + +  +E G+
Sbjct: 63  LKEKLEKTWDENAAKEVGKLIAEKAKEKGITEIVFD-RSGYKYHGKVKALAEAARETGL 120

[Close]

BLAST of Carg27300 vs. ExPASy Swiss-Prot
Match: B1Z777 (50S ribosomal protein L18 OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) OX=441620 GN=rplR PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 7.5e-05
Identity = 28/91 (30.77%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 104 LDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEA 163
           L VF S K ++ +V+     + ++ A++  KDL+ SL +  D  A   +GKL+AER+K A
Sbjct: 29  LSVFRSSKQIYVQVIDDAVGRTLAAASSLDKDLKASLKTGADKAAAEAVGKLVAERAKAA 88

Query: 164 DVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
            V  + ++ R      G++  + D  +E G+
Sbjct: 89  GVTKVVFD-RSGYIFHGRVKALADAAREGGL 118

[Close]

BLAST of Carg27300 vs. ExPASy TrEMBL
Match: A0A6J1FFK7 (uncharacterized protein LOC111445279 OS=Cucurbita moschata OX=3662 GN=LOC111445279 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.6e-98
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS
Sbjct: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 194

[Close]

BLAST of Carg27300 vs. ExPASy TrEMBL
Match: A0A6J1JY30 (uncharacterized protein LOC111489327 OS=Cucurbita maxima OX=3661 GN=LOC111489327 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.3e-89
Identity = 181/194 (93.30%), Postives = 186/194 (95.88%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAF AISSSL   S SSVPSV+SFQ PS+P ILSQKSSSLSWASSFPHVTISRGS VVSS
Sbjct: 1   MAFIAISSSL--PSCSSVPSVLSFQWPSTPTILSQKSSSLSWASSFPHVTISRGSHVVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAHDKVLFI+SAWTRRSREEAAKRPNKKSWKQRTDMYM+PFLLDVFFSKKFVHAKVMHR
Sbjct: 61  NPAHDKVLFIRSAWTRRSREEAAKRPNKKSWKQRTDMYMRPFLLDVFFSKKFVHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERI+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 192

[Close]

BLAST of Carg27300 vs. ExPASy TrEMBL
Match: A0A6J1HH86 (uncharacterized protein LOC111464045 OS=Cucurbita moschata OX=3662 GN=LOC111464045 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.6e-82
Identity = 167/194 (86.08%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSS+   SS+ V S++S QC S+P   SQKSSSLSWASSFP +++SRGSP VSS
Sbjct: 1   MAFTAISSSM--PSSAFVRSILSSQCISTPTTPSQKSSSLSWASSFPSISLSRGSPFVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAH KVLFIQSAWTRRSR EAAKRPNKKSWKQRTDMYM+PFLL+VFFSKKF+HAKVMHR
Sbjct: 61  NPAHAKVLFIQSAWTRRSRGEAAKRPNKKSWKQRTDMYMRPFLLNVFFSKKFMHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERI+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVID IKENGI
Sbjct: 181 KLGIVIDAIKENGI 192

[Close]

BLAST of Carg27300 vs. ExPASy TrEMBL
Match: A0A6J1JHY7 (uncharacterized protein LOC111487153 OS=Cucurbita maxima OX=3661 GN=LOC111487153 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.0e-82
Identity = 168/194 (86.60%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAISSS+   SS+SV S++S QC S+P    QKSSSLSWASSFP +++SRGSP VSS
Sbjct: 1   MAFTAISSSM--PSSASVRSILSSQCISTPT-TPQKSSSLSWASSFPSISLSRGSPFVSS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NPAH KVLFIQSAWTRRSR EAAKRPNKKSWKQRTDMYM+PFLL+VFFSKKF+HAKVMHR
Sbjct: 61  NPAHAKVLFIQSAWTRRSRGEAAKRPNKKSWKQRTDMYMRPFLLNVFFSKKFIHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERI+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 191

[Close]

BLAST of Carg27300 vs. ExPASy TrEMBL
Match: A0A1S3B2Q8 (50S ribosomal protein L18-like OS=Cucumis melo OX=3656 GN=LOC103485371 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.1e-79
Identity = 165/194 (85.05%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MAFTAISSSLQSSSSSSVPSVISFQCPSSPAILSQKSSSLSWASSFPHVTISRGSPVVSS 60
           MAFTAI+SSL S S     SV S Q PS+P    QKSSSLSWASSFPHV I RGSP  SS
Sbjct: 1   MAFTAIASSLPSCS-----SVFSSQGPSTPL---QKSSSLSWASSFPHVNIFRGSPFASS 60

Query: 61  NPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHR 120
           NP + KVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYM+PFLL+VFFSKKF+HAKVMHR
Sbjct: 61  NPPYAKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDMYMRPFLLNVFFSKKFIHAKVMHR 120

Query: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDG 180
           GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYA+SYEPRKDE+I+G
Sbjct: 121 GTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIAERSKEADVYAISYEPRKDEKIEG 180

Query: 181 KLGIVIDTIKENGI 195
           KLGIVIDTIKENGI
Sbjct: 181 KLGIVIDTIKENGI 186

[Close]

BLAST of Carg27300 vs. TAIR 10
Match: AT3G20230.1 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 231.1 bits (588), Expect = 7.6e-61
Identity = 117/157 (74.52%), Postives = 138/157 (87.90%), Query Frame = 0


Query: 38  SSLSWASSFPHVTISRGSPVVSSNPAHDKVLFIQSAWTRRSREEAAKRPNKKSWKQRTDM 97
           S++SW SSFP ++I  GS  V S+P   K  F+Q+AWTRRSR EAAKRPN+KSWKQRTDM
Sbjct: 29  SAVSWQSSFPKLSIEIGS--VISSPIVKKDSFVQAAWTRRSRGEAAKRPNRKSWKQRTDM 88

Query: 98  YMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIA 157
           YM+PFLL+VFFS+KF+HAKVMHR TSKVISVATTNA+D+R ++PSL D+ ACR+IGKLIA
Sbjct: 89  YMRPFLLNVFFSRKFIHAKVMHRPTSKVISVATTNARDIRTNIPSLVDNEACRLIGKLIA 148

Query: 158 ERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
           ERS EADVYA+SYEPRK ERI+GKLGIVIDTIKE+GI
Sbjct: 149 ERSMEADVYAVSYEPRKGERIEGKLGIVIDTIKEHGI 183

[Close]

BLAST of Carg27300 vs. TAIR 10
Match: AT1G08845.1 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 111.3 bits (277), Expect = 8.7e-25
Identity = 56/117 (47.86%), Postives = 83/117 (70.94%), Query Frame = 0


Query: 79  REEAAKRPNKK-SWKQRTDMYMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLR 138
           +E+ +K P+K  S+KQRT  YM+PF LDVF SK+FV A + HR T + ++VA TN+KD++
Sbjct: 66  KEKKSKNPDKHVSFKQRTVAYMEPFTLDVFISKRFVSASLTHRVTCRQVAVAGTNSKDVK 125

Query: 139 NSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
             L S  D  AC  IG++++ER+KEADVY  SY PR  ++ +GK+  V+ ++ +NGI
Sbjct: 126 AVLRSRCDIPACMSIGRILSERAKEADVYTASYTPRDQDKFEGKIRAVVQSLIDNGI 182

[Close]

BLAST of Carg27300 vs. TAIR 10
Match: AT1G08845.2 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 111.3 bits (277), Expect = 8.7e-25
Identity = 56/117 (47.86%), Postives = 83/117 (70.94%), Query Frame = 0


Query: 79  REEAAKRPNKK-SWKQRTDMYMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLR 138
           +E+ +K P+K  S+KQRT  YM+PF LDVF SK+FV A + HR T + ++VA TN+KD++
Sbjct: 111 KEKKSKNPDKHVSFKQRTVAYMEPFTLDVFISKRFVSASLTHRVTCRQVAVAGTNSKDVK 170

Query: 139 NSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
             L S  D  AC  IG++++ER+KEADVY  SY PR  ++ +GK+  V+ ++ +NGI
Sbjct: 171 AVLRSRCDIPACMSIGRILSERAKEADVYTASYTPRDQDKFEGKIRAVVQSLIDNGI 227

[Close]

BLAST of Carg27300 vs. TAIR 10
Match: AT3G22450.1 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 88.6 bits (218), Expect = 6.1e-18
Identity = 46/120 (38.33%), Postives = 75/120 (62.50%), Query Frame = 0


Query: 75  TRRSREEAAKRPNKKSWKQRTDMYMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAK 134
           T    E   KR    ++ Q T  +  PF LD++ SK+ V A V+HR TSKV++VA + +K
Sbjct: 171 TDHPNEAVKKRERTFTFNQLTAPFHYPFCLDIYISKESVRACVIHRVTSKVVTVAHSISK 230

Query: 135 DLRNSLPSLTDHNACRVIGKLIAERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 194
           D++  L S  +  AC  +G ++A+RS E D++ + Y PRK ++I+GKL +V+  + +NG+
Sbjct: 231 DMKFDLGSRRNVAACAAVGAVLAQRSLEDDIHDVIYTPRKGDKIEGKLQVVLQALIDNGV 290

[Close]

BLAST of Carg27300 vs. TAIR 10
Match: AT5G27820.1 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-13
Identity = 36/97 (37.11%), Postives = 62/97 (63.92%), Query Frame = 0


Query: 98  YMKPFLLDVFFSKKFVHAKVMHRGTSKVISVATTNAKDLRNSLPSLTDHNACRVIGKLIA 157
           Y+KP++L + F+ KFVHA+V+H  T+ V   A++  K LR ++    D  A   IGK++ 
Sbjct: 15  YLKPYVLKMHFTNKFVHAQVIHSPTATVACSASSQEKALRETMDITRDVAAASKIGKILG 74

Query: 158 ERSKEADVYAMSYEPRKDERIDGKLGIVIDTIKENGI 195
           ER    D+ A++ + +K++R  GK+  VID+++E G+
Sbjct: 75  ERLLMKDIPAVTIQMKKEQRYHGKVKAVIDSVREAGV 111

The following BLAST results are available for this feature:

BLAST of Carg27300 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015874.1	3.3e-98	100.00	rplR, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022939331.1	3.3e-98	100.00	uncharacterized protein LOC111445279 [Cucurbita moschata]	[more]
XP_023550056.1	1.6e-97	99.48	uncharacterized protein LOC111808358 [Cucurbita pepo subsp. pepo]	[more]
XP_022993255.1	2.8e-89	93.30	uncharacterized protein LOC111489327 [Cucurbita maxima]	[more]
XP_023521951.1	3.0e-83	87.11	uncharacterized protein LOC111785799 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg27300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5P316	1.8e-06	31.09	50S ribosomal protein L18 OS=Aromatoleum aromaticum (strain EbN1) OX=76114 GN=rp...	[more]
B7L0T4	3.4e-05	30.77	50S ribosomal protein L18 OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688)...	[more]
A9W4S4	3.4e-05	30.77	50S ribosomal protein L18 OS=Methylorubrum extorquens (strain PA1) OX=419610 GN=...	[more]
A9BG02	3.4e-05	26.05	50S ribosomal protein L18 OS=Petrotoga mobilis (strain DSM 10674 / SJ95) OX=4038...	[more]
B1Z777	7.5e-05	30.77	50S ribosomal protein L18 OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 1...	[more]

BLAST of Carg27300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FFK7	1.6e-98	100.00	uncharacterized protein LOC111445279 OS=Cucurbita moschata OX=3662 GN=LOC1114452...	[more]
A0A6J1JY30	1.3e-89	93.30	uncharacterized protein LOC111489327 OS=Cucurbita maxima OX=3661 GN=LOC111489327...	[more]
A0A6J1HH86	4.6e-82	86.08	uncharacterized protein LOC111464045 OS=Cucurbita moschata OX=3662 GN=LOC1114640...	[more]
A0A6J1JHY7	6.0e-82	86.60	uncharacterized protein LOC111487153 OS=Cucurbita maxima OX=3661 GN=LOC111487153...	[more]
A0A1S3B2Q8	2.1e-79	85.05	50S ribosomal protein L18-like OS=Cucumis melo OX=3656 GN=LOC103485371 PE=4 SV=1	[more]

BLAST of Carg27300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20230.1	7.6e-61	74.52	Ribosomal L18p/L5e family protein	[more]
AT1G08845.1	8.7e-25	47.86	Ribosomal L18p/L5e family protein	[more]
AT1G08845.2	8.7e-25	47.86	Ribosomal L18p/L5e family protein	[more]
AT3G22450.1	6.1e-18	38.33	Ribosomal L18p/L5e family protein	[more]
AT5G27820.1	1.2e-13	37.11	Ribosomal L18p/L5e family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005484	Ribosomal protein L18	PFAM	PF00861	Ribosomal_L18p	coord: 87..194 e-value: 2.7E-8 score: 34.1
IPR005484	Ribosomal protein L18	PANTHER	PTHR12899	39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL	coord: 9..194
IPR036967	Ribosomal protein S11 superfamily	GENE3D	3.30.420.80	Ribosomal protein S11	coord: 70..194 e-value: 1.1E-14 score: 56.5
None	No IPR available	PANTHER	PTHR12899:SF16	OS02G0689700 PROTEIN	coord: 9..194
None	No IPR available	CDD	cd00432	Ribosomal_L18_L5e	coord: 91..194 e-value: 4.00759E-16 score: 68.7207
None	No IPR available	SUPERFAMILY	53137	Translational machinery components	coord: 98..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27300-RA	Carg27300-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0003735	structural constituent of ribosome