Carg27246 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27246
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEndonuclease III homolog
LocationCarg_Chr14: 11072668 .. 11076040 (+)
RNA-Seq ExpressionCarg27246
SyntenyCarg27246
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAGGTTCTTCAGTAACACACTACACGTTCTATTGTATTTTCCCTCAATGGCCTTGCAGGCACTAGATACTGATGACACAAAACCCACTGTTCTTTATATTTTGTATTTGATATCCGATTATATGAACCAATAACTACAAATTTTACTTCATTTTTAATAGGAAAGAAGATTTGCTGTCTTGGCATCTTCTCTTCTCTCAAGCCAAACCAAAGACCACGTAACTCATGGCAAGTTCTTGTACTCTGAAATTATTAGTTTATAGAGATTGTTCTCTGTTTTTTTTCGTAAGAAACGGGTGTATCTTAACCTGCAGCTATCAAATTCTTCTCTTTTTCCGAACTTTCCTTTACACGTTTTCATGATTCTAGGTTTCCTTTACACCTTTTGTCCGCTTAGGCTAATTACGTATCACCGTCACTCTCATGGTTTTAAAACACACACCTACTAGGGAGAGGTTTCCACACTCTTACGTTCCCCTCTCCAACCGATGTGAGATCTCACAATCCACCCTCTTGGACGCGTTCTAAAACCTTCAGGGGAAGCTCAATGAGGACAATATCTTCTAGTGGTGGGTTTGGACTGTTACATTGGCAAACTACCTATGCACTACCATCACTAACAGTACAAATCGATTTCTCAAGCTGTCTAATCCTGCTATGTGCAAGTCTGTTTGGGTGCAGGAAAGTTTATCAAGAAGCAACAACAGACTCAGAATGATAATGAAATTAGAATGTCAAAATTTTATATTTTGAAAGTAAACTTATTACGTCCCAGGGGCAGTACGTTATTTGTTGTTGCTGCCCGGAGATATTAATCTCTCTCTCTTCTAATATTTTCTTTAATTGACAAAAGATCTTTCAGGAGTGCAGGAGCAGCATTGCGTCTCCAAGAAAGTGGTCTTCTTACAGCTGATGCCATGGACAAAGCTGATGAATCAACCATTAAAAGCTTGATTTACCCTGTACGTTTTCTTGGAAATTATTGAACAATGGCACATAAATTTCCCCATTGCATTTGAATCACATGAATAGAGAAATCTGAAAAATAGCAGTTGTATACTGTGGCTGTTCTGTTCTTGTTGGTGGGGCAGTTTCATTTTCTGTCTATAATTTAGAGTTTTTTGTATCCATCTTGTAGGTTGGATTTTATTCTACAAAGGCTAAAAATTTGAAGAAGATTGCCAAAATATGTCTTATGAAGTATGGTGGGGACATACCTAGATCATTGGAGGAGCTACTTCTACTGCCTGGGATAGGCCCTAAGATTGCACATTTGGTATGACAGATTGTGTATTATTATTAGAAAAGGAGGAACTTATCTGGGTAATGGGTCTGCTTCTCATGGCTCCTCATCATGTTTCATGTGGCATTTAGATCATGATTATGGCGTGGAACGATGTTCAGGGGATATGTGTCGATACTCACGTTCATCGCATTTGCAATCGGCTTGGATGGGTGTCTGGGAAAGGCTCGAAACAGGTAACTGTGGAATTTGTACTGGACTCTACTTGTTTTCTAACATGGATGGATTCACTTTAGATTCGTCCTTAATGGACTTGCCTCCATCAAATGAATCAAATTCTTCCTATTAATTTGAAGTATGCCACGCCTGAGTATCAACATCAATTCCATTAATACTAGTAGAAGGATACCTTACATCAAAATCTGTCTGTTCTAAGTGAAGATGCTCTCAAAGTAAATTAAGAGATTTTGATTCTGAGTAGTTTGGGTAAGGTTAAGGTTGCCATCACCCACACAGATGGTGTGTGATAATCAAGCTATAATGAGTATCACTAAGAATCTTGTTCATCATGATAGGACAAAACGTGTTTGTAACAACTCAAGCCCACTACTAGTAGATATTGTCCGCTTTGGCCCGTTACGTATCGCCGTCAGCCTCACAGTTTTAAAACATGTCTACTAGGAAAAGGTTTTCACACCCTAATAAAGAATGCTTTGTAACCCTCACCCACCGATGTGGGATCTCACAATCCACCACCTCCTTTGGGGTCCAGCGTCCTTGCTGACACACCGCCCAGTGTCTGGCTGTGATACCATTTTGTAACAGCCCAAGACCACCACTAGTAAATATTGTCTGCTTTGGCTTGTTATGTATCGCCGTCAACCTCACGGTTTTAAAGTGCGTCTACTGGGGAGAGATTTCCACACCCTTATAAGGAATGTTTCGTTTCCCTCTCCAATCGATATGGGATCTAGGTCAAAATATGTTGAATTCTCTTGGCACTTCATCTTAGAAAATATCAATTGTGAAGTGATTCAACTCCATTATGTTCCTACTAAGCAACAGATAGTAAGTATTCTCATGAAGTCATTACCTAAACCTAACTTGCTATAATTTGTATAGTTCTTTTCATGGCATTGCTATAATTTGTTTTCTTCTTTCCTTTGCTAGAAAACTTCCTCCCCTGAAGAAACTCGAGTGGCATTAGAACTATGGCTGCCAAAGGAAGAATGGGTTCCAATTAATCCTCTTCTGGTATGTAATGTTTGTTTACAAATAGTTGCTACCCTGAGAACCATGATACAATGAATTTGATGCCATTATTTCCTTATAAGCATACTTGGTTAAACGCAGGACCGAATATTTTCCCTCGAATGTTAATATTCTTAAGACTGTTAAACGTGTCGTAATTGTACGTTTGCATATTCCTATCTGAATTCGTACACGGTGTATAGGATGTTTACCACATGGGAAAACACTCTATTGCATATACAAATGGGTATCTAGGTAACTTCTTATTTATTGCCATTTGCAATAATTTATGCTCCTTATCCATAGAAAATGTGAGATCCCACGTCAGTTGGAGAGGAGAACGAAGCATTTTTTTATAAGGGTGTGGAAACTTCTCTCTGGCAAACGCGTTTTAAAAAACTGAGAGGAAGTCCGAAAGGAAAAGTCCAAAAAGGATAATATTTACTAGCGGTGGGCTTGTGCTTGTATTGTAATAATTTTTTGATTGTTGCCATGATATATAGGTTGGATTTGGACAGACTATTTGCACTCCCCTTAGACCCAAATGTGGAAATTGCAGTGTTAGCCACTTGTGTCCATCTGCATTCAAGGAGACTTCAAGCCCATCTCCCAAATTAAAGCGTTCAAGTTCCACCAAGTAGCTTTGATCATCTTTTTGTATGAATTGGAAGACTTAGCAGCCGCACATTTCCTTTGTGGGGGATCTCATTGGCCATTTTTGTGTAGAACTAACCTTGTTATTGGTATATCAATGTTCTTGGAACATATATTTTGACAAAACTTTCACTACACTGTTTGGCAATTCATTAGGAAAAATTAACTAAATGTCATTGGTTTTAATTTTATGACAAAACTCTTAACATCCAAGATATCATACC

mRNA sequence

CTAAGGTTCTTCAGTAACACACTACACGTTCTATTGTATTTTCCCTCAATGGCCTTGCAGGCACTAGATACTGATGACACAAAACCCACTGTTCTTTATATTTTGTATTTGATATCCGATTATATGAACCAATAACTACAAATTTTACTTCATTTTTAATAGGAAAGAAGATTTGCTGTCTTGGCATCTTCTCTTCTCTCAAGCCAAACCAAAGACCACGTAACTCATGGAGCAGCATTGCGTCTCCAAGAAAGTGGTCTTCTTACAGCTGATGCCATGGACAAAGCTGATGAATCAACCATTAAAAGCTTGATTTACCCTGTTGGATTTTATTCTACAAAGGCTAAAAATTTGAAGAAGATTGCCAAAATATGTCTTATGAAGTATGGTGGGGACATACCTAGATCATTGGAGGAGCTACTTCTACTGCCTGGGATAGGCCCTAAGATTGCACATTTGATCATGATTATGGCGTGGAACGATGTTCAGGGGATATGTGTCGATACTCACGTTCATCGCATTTGCAATCGGCTTGGATGGGTGTCTGGGAAAGGCTCGAAACAGAAAACTTCCTCCCCTGAAGAAACTCGAGTGGCATTAGAACTATGGCTGCCAAAGGAAGAATGGGTTCCAATTAATCCTCTTCTGGTTGGATTTGGACAGACTATTTGCACTCCCCTTAGACCCAAATGTGGAAATTGCAGTGTTAGCCACTTGTGTCCATCTGCATTCAAGGAGACTTCAAGCCCATCTCCCAAATTAAAGCGTTCAAGTTCCACCAAGTAGCTTTGATCATCTTTTTGTATGAATTGGAAGACTTAGCAGCCGCACATTTCCTTTGTGGGGGATCTCATTGGCCATTTTTGTGTAGAACTAACCTTGTTATTGGTATATCAATGTTCTTGGAACATATATTTTGACAAAACTTTCACTACACTGTTTGGCAATTCATTAGGAAAAATTAACTAAATGTCATTGGTTTTAATTTTATGACAAAACTCTTAACATCCAAGATATCATACC

Coding sequence (CDS)

ATGGACAAAGCTGATGAATCAACCATTAAAAGCTTGATTTACCCTGTTGGATTTTATTCTACAAAGGCTAAAAATTTGAAGAAGATTGCCAAAATATGTCTTATGAAGTATGGTGGGGACATACCTAGATCATTGGAGGAGCTACTTCTACTGCCTGGGATAGGCCCTAAGATTGCACATTTGATCATGATTATGGCGTGGAACGATGTTCAGGGGATATGTGTCGATACTCACGTTCATCGCATTTGCAATCGGCTTGGATGGGTGTCTGGGAAAGGCTCGAAACAGAAAACTTCCTCCCCTGAAGAAACTCGAGTGGCATTAGAACTATGGCTGCCAAAGGAAGAATGGGTTCCAATTAATCCTCTTCTGGTTGGATTTGGACAGACTATTTGCACTCCCCTTAGACCCAAATGTGGAAATTGCAGTGTTAGCCACTTGTGTCCATCTGCATTCAAGGAGACTTCAAGCCCATCTCCCAAATTAAAGCGTTCAAGTTCCACCAAGTAG

Protein sequence

MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAHLIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPINPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Homology
BLAST of Carg27246 vs. NCBI nr
Match: KAG7018476.1 (Endonuclease III-like 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 351.7 bits (901), Expect = 3.6e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 169

BLAST of Carg27246 vs. NCBI nr
Match: KAG6582045.1 (Endonuclease III-like 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.7 bits (901), Expect = 3.6e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 211 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 270

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 271 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 330

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 331 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 379

BLAST of Carg27246 vs. NCBI nr
Match: XP_023527414.1 (endonuclease III homolog 1, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.7 bits (901), Expect = 3.6e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 214 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 273

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 274 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 333

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 334 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 382

BLAST of Carg27246 vs. NCBI nr
Match: XP_023527415.1 (endonuclease III homolog 1, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.7 bits (901), Expect = 3.6e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 211 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 270

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 271 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 330

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 331 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 379

BLAST of Carg27246 vs. NCBI nr
Match: XP_022955790.1 (endonuclease III homolog 1, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 350.1 bits (897), Expect = 1.0e-92
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 214 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 273

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDV+GICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 274 LIMIMAWNDVRGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 333

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 334 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 382

BLAST of Carg27246 vs. ExPASy Swiss-Prot
Match: B9DFZ0 (Endonuclease III homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NTH2 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-75
Identity = 132/169 (78.11%), Postives = 149/169 (88.17%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA N+KK+AKICLM+Y GDIPR+LEELL LPG+GPKIAH
Sbjct: 216 IDKADESTIKELIYPVGFYTRKATNVKKVAKICLMEYDGDIPRTLEELLSLPGVGPKIAH 275

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           L++ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQKTSSPEETRVAL+ WLPK EWV I
Sbjct: 276 LVLHVAWNDVQGICVDTHVHRICNRLGWVSKPGTKQKTSSPEETRVALQQWLPKGEWVAI 335

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           N LLVGFGQTICTPLRP CG CS++ +CPSAFKET S S KLK+S  +K
Sbjct: 336 NFLLVGFGQTICTPLRPHCGTCSITEICPSAFKETPSTSSKLKKSIKSK 384

BLAST of Carg27246 vs. ExPASy Swiss-Prot
Match: Q9SIC4 (Endonuclease III homolog 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NTH1 PE=2 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 4.6e-75
Identity = 132/169 (78.11%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA  +KKIA+ICL+KY GDIP SL++LL LPGIGPK+AH
Sbjct: 209 VDKADESTIKELIYPVGFYTRKATYMKKIARICLVKYDGDIPSSLDDLLSLPGIGPKMAH 268

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LI+ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQKT+SPEETRVAL+ WLPKEEWV I
Sbjct: 269 LILHIAWNDVQGICVDTHVHRICNRLGWVSRPGTKQKTTSPEETRVALQQWLPKEEWVAI 328

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQ ICTP+RP+C  CSVS LCP+AFKETSSPS KLK+S+ +K
Sbjct: 329 NPLLVGFGQMICTPIRPRCEACSVSKLCPAAFKETSSPSSKLKKSNRSK 377

BLAST of Carg27246 vs. ExPASy Swiss-Prot
Match: Q2KID2 (Endonuclease III-like protein 1 OS=Bos taurus OX=9913 GN=NTHL1 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-43
Identity = 84/149 (56.38%), Postives = 105/149 (70.47%), Query Frame = 0

Query: 3   KADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAHLI 62
           + D+ST+ +LIYPVGF+ +K K +K+ + I   +Y GDIP S+ EL+ LPG+GPK+AHL 
Sbjct: 159 QTDDSTLGALIYPVGFWRSKVKYIKQTSAILQQRYDGDIPASVAELVALPGVGPKMAHLA 218

Query: 63  MIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPINP 122
           M +AW  V GI VDTHVHRI NRL W     +K+ T SPEETR ALE WLP+E W  IN 
Sbjct: 219 MAVAWGTVSGIAVDTHVHRIANRLRW-----TKKATKSPEETRRALEEWLPRELWSEING 278

Query: 123 LLVGFGQTICTPLRPKCGNCSVSHLCPSA 152
           LLVGFGQ  C P+RP+C  C    LCP+A
Sbjct: 279 LLVGFGQQTCLPIRPRCQACLNRALCPAA 302

BLAST of Carg27246 vs. ExPASy Swiss-Prot
Match: A7M7B9 (Endonuclease III-like protein 1 OS=Gallus gallus OX=9031 GN=NTHL1 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-43
Identity = 83/147 (56.46%), Postives = 104/147 (70.75%), Query Frame = 0

Query: 5   DESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAHLIMI 64
           D++T+  +IYPVGF+  K K +K+   I   KYGGDIP ++EEL+ LPG+GPK+AHL M 
Sbjct: 137 DDATLGQIIYPVGFWRNKVKYIKQTTAILKQKYGGDIPGTVEELVKLPGVGPKMAHLAMN 196

Query: 65  MAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPINPLL 124
           +AWN V GI VDTHVHRI NRL WV     K++T  PEETRVALE WLP++ W  IN LL
Sbjct: 197 IAWNSVSGIAVDTHVHRITNRLKWV-----KKETRYPEETRVALEDWLPRDLWREINWLL 256

Query: 125 VGFGQTICTPLRPKCGNCSVSHLCPSA 152
           VGFGQ  C P+ P+C  C    +CP+A
Sbjct: 257 VGFGQQTCLPVNPRCKECLNQDICPTA 278

BLAST of Carg27246 vs. ExPASy Swiss-Prot
Match: P78549 (Endonuclease III-like protein 1 OS=Homo sapiens OX=9606 GN=NTHL1 PE=1 SV=2)

HSP 1 Score: 176.4 bits (446), Expect = 2.7e-43
Identity = 83/149 (55.70%), Postives = 103/149 (69.13%), Query Frame = 0

Query: 3   KADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAHLI 62
           + D++T+  LIYPVGF+ +K K +K+ + I    YGGDIP S+ EL+ LPG+GPK+AHL 
Sbjct: 166 QTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLA 225

Query: 63  MIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPINP 122
           M +AW  V GI VDTHVHRI NRL W     +K+ T SPEETR ALE WLP+E W  IN 
Sbjct: 226 MAVAWGTVSGIAVDTHVHRIANRLRW-----TKKATKSPEETRAALEEWLPRELWHEING 285

Query: 123 LLVGFGQTICTPLRPKCGNCSVSHLCPSA 152
           LLVGFGQ  C P+ P+C  C    LCP+A
Sbjct: 286 LLVGFGQQTCLPVHPRCHACLNQALCPAA 309

BLAST of Carg27246 vs. ExPASy TrEMBL
Match: A0A6J1GW15 (Endonuclease III homolog OS=Cucurbita moschata OX=3662 GN=LOC111457673 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.1e-93
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 214 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 273

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDV+GICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 274 LIMIMAWNDVRGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 333

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 334 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 382

BLAST of Carg27246 vs. ExPASy TrEMBL
Match: A0A6J1GUU3 (Endonuclease III homolog OS=Cucurbita moschata OX=3662 GN=LOC111457673 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.1e-93
Identity = 168/169 (99.41%), Postives = 169/169 (100.00%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 211 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 270

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDV+GICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 271 LIMIMAWNDVRGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 330

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK
Sbjct: 331 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 379

BLAST of Carg27246 vs. ExPASy TrEMBL
Match: A0A6J1IUE8 (Endonuclease III homolog OS=Cucurbita maxima OX=3661 GN=LOC111479404 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.5e-92
Identity = 167/169 (98.82%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 213 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 272

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 273 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 332

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAF E SSPSPKLKRSSSTK
Sbjct: 333 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFNEASSPSPKLKRSSSTK 381

BLAST of Carg27246 vs. ExPASy TrEMBL
Match: A0A6J1IRV0 (Endonuclease III homolog OS=Cucurbita maxima OX=3661 GN=LOC111479404 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.5e-92
Identity = 167/169 (98.82%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 210 MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 269

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI
Sbjct: 270 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 329

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAF E SSPSPKLKRSSSTK
Sbjct: 330 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFNEASSPSPKLKRSSSTK 378

BLAST of Carg27246 vs. ExPASy TrEMBL
Match: A0A5A7TN21 (Endonuclease III homolog OS=Cucumis melo var. makuwa OX=1194695 GN=NTH1 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.8e-90
Identity = 163/169 (96.45%), Postives = 165/169 (97.63%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           MDKADE TIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH
Sbjct: 219 MDKADEETIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 278

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTS+PEETRV LELWLPKEEWVPI
Sbjct: 279 LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSTPEETRVGLELWLPKEEWVPI 338

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQTICTPLRPKCGNCSVS LCPSAFKE+SSPSPKLK SSSTK
Sbjct: 339 NPLLVGFGQTICTPLRPKCGNCSVSDLCPSAFKESSSPSPKLKGSSSTK 387

BLAST of Carg27246 vs. TAIR 10
Match: AT1G05900.2 (endonuclease III 2 )

HSP 1 Score: 282.7 bits (722), Expect = 1.9e-76
Identity = 132/169 (78.11%), Postives = 149/169 (88.17%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA N+KK+AKICLM+Y GDIPR+LEELL LPG+GPKIAH
Sbjct: 216 IDKADESTIKELIYPVGFYTRKATNVKKVAKICLMEYDGDIPRTLEELLSLPGVGPKIAH 275

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           L++ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQKTSSPEETRVAL+ WLPK EWV I
Sbjct: 276 LVLHVAWNDVQGICVDTHVHRICNRLGWVSKPGTKQKTSSPEETRVALQQWLPKGEWVAI 335

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           N LLVGFGQTICTPLRP CG CS++ +CPSAFKET S S KLK+S  +K
Sbjct: 336 NFLLVGFGQTICTPLRPHCGTCSITEICPSAFKETPSTSSKLKKSIKSK 384

BLAST of Carg27246 vs. TAIR 10
Match: AT2G31450.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 3.3e-76
Identity = 132/169 (78.11%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA  +KKIA+ICL+KY GDIP SL++LL LPGIGPK+AH
Sbjct: 209 VDKADESTIKELIYPVGFYTRKATYMKKIARICLVKYDGDIPSSLDDLLSLPGIGPKMAH 268

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LI+ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQKT+SPEETRVAL+ WLPKEEWV I
Sbjct: 269 LILHIAWNDVQGICVDTHVHRICNRLGWVSRPGTKQKTTSPEETRVALQQWLPKEEWVAI 328

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQ ICTP+RP+C  CSVS LCP+AFKETSSPS KLK+S+ +K
Sbjct: 329 NPLLVGFGQMICTPIRPRCEACSVSKLCPAAFKETSSPSSKLKKSNRSK 377

BLAST of Carg27246 vs. TAIR 10
Match: AT2G31450.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 3.3e-76
Identity = 132/169 (78.11%), Postives = 151/169 (89.35%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA  +KKIA+ICL+KY GDIP SL++LL LPGIGPK+AH
Sbjct: 207 VDKADESTIKELIYPVGFYTRKATYMKKIARICLVKYDGDIPSSLDDLLSLPGIGPKMAH 266

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPI 120
           LI+ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQKT+SPEETRVAL+ WLPKEEWV I
Sbjct: 267 LILHIAWNDVQGICVDTHVHRICNRLGWVSRPGTKQKTTSPEETRVALQQWLPKEEWVAI 326

Query: 121 NPLLVGFGQTICTPLRPKCGNCSVSHLCPSAFKETSSPSPKLKRSSSTK 170
           NPLLVGFGQ ICTP+RP+C  CSVS LCP+AFKETSSPS KLK+S+ +K
Sbjct: 327 NPLLVGFGQMICTPIRPRCEACSVSKLCPAAFKETSSPSSKLKKSNRSK 375

BLAST of Carg27246 vs. TAIR 10
Match: AT1G05900.1 (endonuclease III 2 )

HSP 1 Score: 171.4 bits (433), Expect = 6.2e-43
Identity = 77/96 (80.21%), Postives = 88/96 (91.67%), Query Frame = 0

Query: 1   MDKADESTIKSLIYPVGFYSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAH 60
           +DKADESTIK LIYPVGFY+ KA N+KK+AKICLM+Y GDIPR+LEELL LPG+GPKIAH
Sbjct: 216 IDKADESTIKELIYPVGFYTRKATNVKKVAKICLMEYDGDIPRTLEELLSLPGVGPKIAH 275

Query: 61  LIMIMAWNDVQGICVDTHVHRICNRLGWVSGKGSKQ 97
           L++ +AWNDVQGICVDTHVHRICNRLGWVS  G+KQ
Sbjct: 276 LVLHVAWNDVQGICVDTHVHRICNRLGWVSKPGTKQ 311

BLAST of Carg27246 vs. TAIR 10
Match: AT4G12740.1 (HhH-GPD base excision DNA repair family protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.6e-06
Identity = 38/130 (29.23%), Postives = 62/130 (47.69%), Query Frame = 0

Query: 19  YSTKAKNLKKIAKICLMKYGGDIPRSLEELLLLPGIGPKIAHLIMIMAWNDVQGICVDTH 78
           Y  +A+ L + AK+ +    G  P     L+ + GIG   A  I  +A+N+   + VD +
Sbjct: 232 YYRRARFLLEGAKMVVAGTEG-FPNQASSLMKVKGIGQYTAGAIASIAFNEAVPV-VDGN 291

Query: 79  VHRICNRLGWVSGKGSKQKTSSPEETRVALELWLPKEEWVPINPLLVGFGQTICTPLRPK 138
           V R+  RL  +S    K + ++    ++A +L  P       N  L+  G T+CT  +P 
Sbjct: 292 VIRVLARLKAISA-NPKDRLTARNFWKLAAQLVDPSRPG-DFNQSLMELGATLCTVSKPS 351

Query: 139 CGNCSVSHLC 149
           C +C VS  C
Sbjct: 352 CSSCPVSSQC 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018476.13.6e-93100.00Endonuclease III-like 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6582045.13.6e-93100.00Endonuclease III-like 2, chloroplastic, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023527414.13.6e-93100.00endonuclease III homolog 1, chloroplastic-like isoform X1 [Cucurbita pepo subsp.... [more]
XP_023527415.13.6e-93100.00endonuclease III homolog 1, chloroplastic-like isoform X2 [Cucurbita pepo subsp.... [more]
XP_022955790.11.0e-9299.41endonuclease III homolog 1, chloroplastic-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9DFZ02.7e-7578.11Endonuclease III homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NTH... [more]
Q9SIC44.6e-7578.11Endonuclease III homolog 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NTH... [more]
Q2KID21.2e-4356.38Endonuclease III-like protein 1 OS=Bos taurus OX=9913 GN=NTHL1 PE=2 SV=1[more]
A7M7B91.2e-4356.46Endonuclease III-like protein 1 OS=Gallus gallus OX=9031 GN=NTHL1 PE=2 SV=1[more]
P785492.7e-4355.70Endonuclease III-like protein 1 OS=Homo sapiens OX=9606 GN=NTHL1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GW155.1e-9399.41Endonuclease III homolog OS=Cucurbita moschata OX=3662 GN=LOC111457673 PE=3 SV=1[more]
A0A6J1GUU35.1e-9399.41Endonuclease III homolog OS=Cucurbita moschata OX=3662 GN=LOC111457673 PE=3 SV=1[more]
A0A6J1IUE82.5e-9298.82Endonuclease III homolog OS=Cucurbita maxima OX=3661 GN=LOC111479404 PE=3 SV=1[more]
A0A6J1IRV02.5e-9298.82Endonuclease III homolog OS=Cucurbita maxima OX=3661 GN=LOC111479404 PE=3 SV=1[more]
A0A5A7TN216.8e-9096.45Endonuclease III homolog OS=Cucumis melo var. makuwa OX=1194695 GN=NTH1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G05900.21.9e-7678.11endonuclease III 2 [more]
AT2G31450.13.3e-7678.11DNA glycosylase superfamily protein [more]
AT2G31450.23.3e-7678.11DNA glycosylase superfamily protein [more]
AT1G05900.16.2e-4380.21endonuclease III 2 [more]
AT4G12740.14.6e-0629.23HhH-GPD base excision DNA repair family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003265HhH-GPD domainSMARTSM00478endo3endcoord: 2..130
e-value: 6.3E-37
score: 138.7
IPR003265HhH-GPD domainPFAMPF00730HhH-GPDcoord: 3..98
e-value: 5.5E-15
score: 56.1
IPR003265HhH-GPD domainCDDcd00056ENDO3ccoord: 1..128
e-value: 1.38217E-41
score: 135.062
IPR003651Endonuclease III-like, iron-sulphur cluster loop motifSMARTSM00525ccc3coord: 131..151
e-value: 3.4E-5
score: 33.3
IPR000445Helix-hairpin-helix motifPFAMPF00633HHHcoord: 36..63
e-value: 1.4E-8
score: 34.2
NoneNo IPR availableGENE3D1.10.340.30Hypothetical protein; domain 2coord: 1..63
e-value: 3.8E-20
score: 74.4
NoneNo IPR availablePIRSFPIRSF001435Nthcoord: 1..157
e-value: 3.0E-27
score: 93.2
NoneNo IPR availablePANTHERPTHR43286ENDONUCLEASE III-LIKE PROTEIN 1coord: 2..165
NoneNo IPR availablePANTHERPTHR43286:SF3ENDONUCLEASE III HOMOLOG 2, CHLOROPLASTICcoord: 2..165
IPR023170Helix-hairpin-helix, base-excision DNA repair, C-terminalGENE3D1.10.1670.10coord: 64..154
e-value: 4.9E-26
score: 92.6
IPR004036Endonuclease III-like, conserved site-2PROSITEPS01155ENDONUCLEASE_III_2coord: 39..68
IPR004035Endonuclease III, iron-sulphur binding sitePROSITEPS00764ENDONUCLEASE_III_1coord: 132..148
IPR011257DNA glycosylaseSUPERFAMILY48150DNA-glycosylasecoord: 3..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27246-RACarg27246-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006285 base-excision repair, AP site formation
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006284 base-excision repair
biological_process GO:0006281 DNA repair
cellular_component GO:0005634 nucleus
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0140078 class I DNA-(apurinic or apyrimidinic site) endonuclease activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000703 oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity
molecular_function GO:0003824 catalytic activity