Carg27194 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27194
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Carg_Chr11: 2158440 .. 2159793 (+)
RNA-Seq Expression	Carg27194
Synteny	Carg27194

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGTCTGGTTTTTGTAAATTTTTTGAATTTTTCTCTCTCTATGGAGCGAATAGATGATTGATGTGTCTGGTGAGGGTTGCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGTCAGCTTCATTCTAGAAAGCATTTAACACAACATTCATAAACATTAAATGGTTACCCTTTTTTGGTACAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGGTAAGTTTCAAGTGCAAGTTTTAAGGTTGTCCTTACATTCATGTGTGTGCCTCTTATGTTATTGTGTATCTGTGCAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAAAAAACATCAACACTAATCTCTGTTTTTTGTACTGCTTTCACTGAATGTTTCAATAATAGGCTGTTCT

mRNA sequence

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAAAAAACATCAACACTAATCTCTGTTTTTTGTACTGCTTTCACTGAATGTTTCAATAATAGGCTGTTCT

Coding sequence (CDS)

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAA

Protein sequence

MTQKSHPQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI

Homology

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BLAST of Carg27194 vs. NCBI nr
Match: KAG6587867.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021754.1 Protein SRG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.5 bits (1856), Expect = 1.4e-203
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MTQKSHPQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQG 60
           MTQKSHPQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQG
Sbjct: 1   MTQKSHPQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQG 60

Query: 61  GEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEG 120
           GEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEG
Sbjct: 61  GEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEG 120

Query: 121 YGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVL 180
           YGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVL
Sbjct: 121 YGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVL 180

Query: 181 IAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKE 240
           IAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKE
Sbjct: 181 IAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKE 240

Query: 241 VEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVAC 300
           VEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVAC
Sbjct: 241 VEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVAC 300

Query: 301 FCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           FCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI
Sbjct: 301 FCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 352

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BLAST of Carg27194 vs. NCBI nr
Match: XP_011655698.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653028.1 hypothetical protein Csa_020037 [Cucumis sativus])

HSP 1 Score: 566.6 bits (1459), Expect = 1.5e-157
Identity = 273/345 (79.13%), Postives = 308/345 (89.28%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSSSQGGEAALEE 68
           QTLQQQLLINGG TPESYIYKGGY GG SNN+ PLP+AEIPVVDLSQLSS   GE  L +
Sbjct: 11  QTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSSPSAGEGPLND 70

Query: 69  FRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLIL 128
            RLALS+WGCFQA NH ISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG EGYG+DLIL
Sbjct: 71  LRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLIL 130

Query: 129 SEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASL 188
           SE QILDW+DRLY V NPEDER+L+ WP NPPSFREDL E+T+K+ EIIE VLIAMA+SL
Sbjct: 131 SEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDEYTVKIMEIIETVLIAMASSL 190

Query: 189 KVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMK 248
            VEP SF+DQVGKRP L+TRFNFYPPCSTPHLVLGLKEHSDGSA TI+LLDK+VEGL+++
Sbjct: 191 DVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLR 250

Query: 249 KDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKD 308
           KD+QWYR+PVPAIADSLLI +GEQ E+MSNGIFKS +HRAVTNSERQRIS+ CFCCPEKD
Sbjct: 251 KDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQRISLVCFCCPEKD 310

Query: 309 KEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            EIKPIEGLIDE+RPRLF++VKNY+ ETYFQ YQKG+R VD L+I
Sbjct: 311 IEIKPIEGLIDEKRPRLFKSVKNYL-ETYFQNYQKGRRPVDGLRI 354

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BLAST of Carg27194 vs. NCBI nr
Match: XP_008465534.1 (PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 563.1 bits (1450), Expect = 1.6e-156
Identity = 275/355 (77.46%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIE 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSFREDLHE+T+K+ +II+
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKIID 180

Query: 181 NVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLL 240
            VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++LL
Sbjct: 181 TVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLLL 240

Query: 241 DKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRIS 300
           DK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRIS
Sbjct: 241 DKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRIS 300

Query: 301 VACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           V  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 VVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 354

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BLAST of Carg27194 vs. NCBI nr
Match: TYK10347.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 561.2 bits (1445), Expect = 6.2e-156
Identity = 277/356 (77.81%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSF-REDLHEFTIKVKEII 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSF REDLHE+T+K+ EII
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFSREDLHEYTVKLMEII 180

Query: 181 ENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVL 240
           E VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++L
Sbjct: 181 ETVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLL 240

Query: 241 LDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRI 300
           LDK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRI
Sbjct: 241 LDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRI 300

Query: 301 SVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           SV  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 SVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 355

[Close]

BLAST of Carg27194 vs. NCBI nr
Match: XP_011655280.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucumis sativus] >KAE8653014.1 hypothetical protein Csa_004557 [Cucumis sativus])

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-155
Identity = 269/345 (77.97%), Postives = 303/345 (87.83%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSSSQGGEAALEE 68
           QTLQQQLLINGG TPESYIYKGGY GG SNN+ PLP+AEIPVVDLSQLSS   GE  L +
Sbjct: 11  QTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSSPSAGEGPLND 70

Query: 69  FRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLIL 128
            RLALS+WGCFQATNH ISSSFLEK+R+ISEQFF+LP+EEK RY RE+DG EGYG+DL  
Sbjct: 71  LRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLTF 130

Query: 129 SEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASL 188
           S  Q LDW+DRLY V +PEDER+L  WP NPPSFREDLHE+T+K+ EIIE VLIAMA SL
Sbjct: 131 SNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIMEIIETVLIAMARSL 190

Query: 189 KVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMK 248
            VEP SF+DQVG+RP L TRFNFYPPCSTPHLVLGLKEHSDGSA TI+LLDK+VEGL+++
Sbjct: 191 NVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLR 250

Query: 249 KDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKD 308
           KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS VHRAVTNSERQRISV CFCCPEKD
Sbjct: 251 KDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQRISVVCFCCPEKD 310

Query: 309 KEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            EIKP+EGLIDE+RPRLFR+VKNY+ ETYFQ YQ+GQR VD L+I
Sbjct: 311 IEIKPVEGLIDEKRPRLFRSVKNYL-ETYFQNYQEGQRTVDGLRI 354

[Close]

BLAST of Carg27194 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.9e-56
Identity = 112/312 (35.90%), Postives = 186/312 (59.62%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIP++D+ +L SS   ++ +E+   A   WG FQ  NHGI SSFL+K++   + FF LPM
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPM 111

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           EEK ++++  D  EG+G   ++SE Q LDW D  +  V P + RK   +P+ P  FR+ L
Sbjct: 112 EEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTL 171

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFS---DQVGKRPVLVTRFNFYPPCSTPHLVLG 225
             ++ +V+ + + ++  MA +L+++P+      D V    V   R N+YPPC  P  V+G
Sbjct: 172 EMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDS--VQSMRMNYYPPCPQPDQVIG 231

Query: 226 LKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKS 285
           L  HSD    T+++   +VEGL++KKD +W  +PV  + ++ ++N+G+  EI++NG ++S
Sbjct: 232 LTPHSDSVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLPNAFIVNIGDVLEIITNGTYRS 291

Query: 286 PVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFR--TVKNYVEETYFQYY 345
             HR V NSE++R+S+A F      KE+ P + L++ ++   F+  T+K Y  +  F   
Sbjct: 292 IEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEY-NDGLFSRT 351

Query: 346 QKGQRAVDELKI 353
             G+  +D L+I
Sbjct: 352 LDGKAYLDALRI 358

[Close]

BLAST of Carg27194 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.8e-52
Identity = 106/274 (38.69%), Postives = 162/274 (59.12%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIPV+DLS+L   Q     L +F  A   WG FQ  NHG+    +EKM+  +E FF LP 
Sbjct: 50  EIPVIDLSRLLDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPF 109

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           +EKN Y +  +G EGYG   + SE Q LDW D  +L+  P  ER ++FWP +P SFRE +
Sbjct: 110 KEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETM 169

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKE 225
            +++++++++   +   MA +L +E +  +  +  R V        P  S+    LGL  
Sbjct: 170 EKYSMELQKVAMCLTGMMAKNLGLESEILTKPL--RTVFNREDELLPSMSSCGEGLGLSP 229

Query: 226 HSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVH 285
           HSD +  T+++   EV GL +KKD +W  +P+  I  + ++N+G+  EIMSNGI+KS  H
Sbjct: 230 HSDATGLTLLIQVNEVNGLHIKKDEKW--VPIKPILGAFVVNIGDVIEIMSNGIYKSIEH 289

Query: 286 RAVTNSERQRISVACFCCPEKDKEIKPIEGLIDE 320
           RAV N++++R+S+A F  PE   +I P+  L+ E
Sbjct: 290 RAVINTDKERLSIAAFHDPEYGTKIGPLPDLVKE 319

[Close]

BLAST of Carg27194 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.1e-52
Identity = 102/286 (35.66%), Postives = 165/286 (57.69%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIPV+D++ +    G    L   R A   WG FQ  NHG++ S +E++R    +FF LP+
Sbjct: 47  EIPVLDMNDV---WGKPEGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPL 106

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           EEK +Y    D +EGYGS L + +   LDW+D  +L   P   R    WP  PP  RE +
Sbjct: 107 EEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELI 166

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFSDQV--GKRPVLVTRFNFYPPCSTPHLVLGL 225
            ++  +V+++ E +   ++ SL ++P      +  G +     R NFYP C  P L LGL
Sbjct: 167 EKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGL 226

Query: 226 KEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSP 285
             HSD    TI+L D++V GL++++ + W  + + ++ ++L++N+G+Q +I+SNGI+KS 
Sbjct: 227 SSHSDPGGITILLPDEKVAGLQVRRGDGW--VTIKSVPNALIVNIGDQLQILSNGIYKSV 286

Query: 286 VHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVK 330
            H+ + NS  +R+S+A F  P  D  + PIE L+   RP L++ ++
Sbjct: 287 EHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIR 327

[Close]

BLAST of Carg27194 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.6e-51
Identity = 112/332 (33.73%), Postives = 180/332 (54.22%), Query Frame = 0


Query: 23  PESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATN 82
           PE YI     S    NN    MA IP++DL +L   Q  E    + R A   WG F   N
Sbjct: 30  PERYIRPEASSEEVINNYQGDMA-IPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLLIN 89

Query: 83  HGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLV 142
           HG+    +  +++    FF+ P++ K  Y +  +  EGYG   + SE Q LDW D LYL 
Sbjct: 90  HGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLYLH 149

Query: 143 VNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRP 202
           V+P D R L+FWP +P SFR+ +  ++ + K +   +   MA ++  +P+S  D   ++P
Sbjct: 150 VHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEEQP 209

Query: 203 VLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIAD 262
               R  +YPPC     V+GL  HSD    T++L    V+GL++KKD +W+ +  P    
Sbjct: 210 -RGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAP--NG 269

Query: 263 SLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRP 322
           +L+ N+G+  EI+SNG F+S  HRAV N  ++RIS A F  P ++  I P+   + + + 
Sbjct: 270 ALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKV 329

Query: 323 RLFRTVK--NYVEETYFQYYQKGQRAVDELKI 353
           + +R++   +++++ + Q    G+  V+ LK+
Sbjct: 330 K-YRSISYLDFMKQIFTQQLD-GKNRVEVLKL 355

[Close]

BLAST of Carg27194 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.3e-50
Identity = 109/283 (38.52%), Postives = 165/283 (58.30%), Query Frame = 0


Query: 47  IPVVDLSQLSSSQG--GEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALP 106
           +PV+DL  L S +   G+  L++   A   WG FQ  NHG+ +  ++ ++   + FF LP
Sbjct: 54  VPVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLP 113

Query: 107 MEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFRED 166
           M EK +Y ++    EG+G   I SE Q LDWT+   ++  P   RK   +PE P  FRE 
Sbjct: 114 MNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRET 173

Query: 167 LHEFTIKVKEIIENVLIAMAASLK-VEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGL 226
           L  +  K+K++   V   +  SL+ VE K  +D + +  +   R N+YPPC  P LVLGL
Sbjct: 174 LESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTD-LFEDGLQTMRMNYYPPCPRPELVLGL 233

Query: 227 KEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSP 286
             HSD S  TI+L   EVEGL+++K+ +W  + +  + D+ ++NVG+  EIM+NGI++S 
Sbjct: 234 TSHSDFSGLTILLQLNEVEGLQIRKEERW--ISIKPLPDAFIVNVGDILEIMTNGIYRSV 293

Query: 287 VHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFR 327
            HRAV NS ++R+S+A F   + + EI PI  L+    P LF+
Sbjct: 294 EHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFK 333

[Close]

BLAST of Carg27194 vs. ExPASy TrEMBL
Match: A0A1S3CP07 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.9e-157
Identity = 275/355 (77.46%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIE 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSFREDLHE+T+K+ +II+
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKIID 180

Query: 181 NVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLL 240
            VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++LL
Sbjct: 181 TVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLLL 240

Query: 241 DKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRIS 300
           DK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRIS
Sbjct: 241 DKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRIS 300

Query: 301 VACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           V  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 VVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 354

[Close]

BLAST of Carg27194 vs. ExPASy TrEMBL
Match: A0A5D3CEG2 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00240 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 3.0e-156
Identity = 277/356 (77.81%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSF-REDLHEFTIKVKEII 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSF REDLHE+T+K+ EII
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFSREDLHEYTVKLMEII 180

Query: 181 ENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVL 240
           E VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++L
Sbjct: 181 ETVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLL 240

Query: 241 LDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRI 300
           LDK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRI
Sbjct: 241 LDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRI 300

Query: 301 SVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           SV  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 SVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 355

[Close]

BLAST of Carg27194 vs. ExPASy TrEMBL
Match: A0A5A7U2F3 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00670 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.0e-155
Identity = 276/356 (77.53%), Postives = 313/356 (87.92%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSF-REDLHEFTIKVKEII 180
            EGYG+DLI S  QILDW+DRLYLV NPED R+L+FWP NPPSF REDLHE+T+K+ EII
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPEDGRRLEFWPLNPPSFSREDLHEYTVKLMEII 180

Query: 181 ENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVL 240
           E VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++L
Sbjct: 181 ETVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLL 240

Query: 241 LDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRI 300
           LDK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSER+RI
Sbjct: 241 LDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERRRI 300

Query: 301 SVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           SV  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 SVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 355

[Close]

BLAST of Carg27194 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.4e-155
Identity = 267/344 (77.62%), Postives = 305/344 (88.66%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEF 68
           +T QQ+LLINGGDTPESYIYK GY GGDSNN+PLP+AEIPVVDL+QLSSS    AALE+ 
Sbjct: 363 KTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALEDL 422

Query: 69  RLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILS 128
           RLAL+SWGCFQA NH ISSSFL K+ QIS QFF+LPMEEKN+  RE+ G EGYG+D++ S
Sbjct: 423 RLALTSWGCFQAINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFS 482

Query: 129 EPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLK 188
           E QILDWTDRLYL VNPEDER+LK+WP+NP SFREDLHEFTIK+K+IIE VL+AMA S+ 
Sbjct: 483 EQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSIN 542

Query: 189 VEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKK 248
           VE  SF++QVGKRP L TRFNFYPPCS P LVLGLKEHSDGSA TIVLLD+EVEGL+ +K
Sbjct: 543 VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRK 602

Query: 249 DNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDK 308
           D+QW+R+PVPA+ADSLLIN+GEQ EIMSNG+FKS VHRAVTNSE+QRISVACFCCPEKD+
Sbjct: 603 DDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDR 662

Query: 309 EIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           EI+PIEGLIDERRPRL+R VKNYV  +YFQ YQKGQR VD+LKI
Sbjct: 663 EIEPIEGLIDERRPRLYRNVKNYV-GSYFQDYQKGQRPVDKLKI 705

[Close]

BLAST of Carg27194 vs. ExPASy TrEMBL
Match: A0A0A0LW96 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212830 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.3e-132
Identity = 227/298 (76.17%), Postives = 259/298 (86.91%), Query Frame = 0


Query: 55  LSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYRE 114
           L S   GE  L + RLALS+WGCFQATNH ISSSFLEK+R+ISEQFF+LP+EEK RY RE
Sbjct: 281 LKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGRE 340

Query: 115 IDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKE 174
           +DG EGYG+DL  S  Q LDW+DRLY V +PEDER+L  WP NPPSFREDLHE+T+K+ E
Sbjct: 341 VDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIME 400

Query: 175 IIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTI 234
           IIE VLIAMA SL VEP SF+DQVG+RP L TRFNFYPPCSTPHLVLGLKEHSDGSA TI
Sbjct: 401 IIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITI 460

Query: 235 VLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQ 294
           +LLDK+VEGL+++KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS VHRAVTNSERQ
Sbjct: 461 LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQ 520

Query: 295 RISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           RISV CFCCPEKD EIKP+EGLIDE+RPRLFR+VKNY+ ETYFQ YQ+GQR VD L+I
Sbjct: 521 RISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYL-ETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of Carg27194 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.6e-99
Identity = 176/347 (50.72%), Postives = 243/347 (70.03%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPL----PMAEIPVVDLS-QLSSSQGG 66
           P+    Q+++  G   PE Y++      GD    PL    P  +IP +DL+  LSSS+ G
Sbjct: 4   PKFKTVQEVVAAGEGLPERYLHA---PTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAG 63

Query: 67  EAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGY 126
           +  L +   ALS+WG  Q  NHGI+ +FL+K+ +++++FFALP EEK +  REID  +GY
Sbjct: 64  QQELSKLHSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGY 123

Query: 127 GSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLI 186
           G+D+IL + Q+LDW DRLY+   PED+R+L FWPE P  FRE LHE+T+K + +IE    
Sbjct: 124 GNDMILWDDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFK 183

Query: 187 AMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEV 246
           AMA SL++E  SF D  G+   L TRFN YPPC +P  V+G+K H+DGSA T++L DK+V
Sbjct: 184 AMARSLELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDV 243

Query: 247 EGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACF 306
            GL+ +KD +WY+ P+  + D++LINVG+Q EIMSNGI+KSPVHR VTN E++RISVA F
Sbjct: 244 GGLQFQKDGKWYKAPI--VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATF 303

Query: 307 CCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVD 349
           C P  DKEI+P+  L+ E RPRL++TVK YV E YF+YYQ+G+R ++
Sbjct: 304 CIPGADKEIQPVNELVSEARPRLYKTVKKYV-ELYFKYYQQGRRPIE 344

[Close]

BLAST of Carg27194 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.5e-96
Identity = 168/349 (48.14%), Postives = 236/349 (67.62%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIY--KGGYSGGDSNNDPLPMAEIPVVDLSQL-SSSQGGEA 66
           P+    Q+++  G   PE Y+Y   G   G    N  LP  +I ++DL+ L SSS  G  
Sbjct: 4   PKLKTVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGRE 63

Query: 67  ALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGS 126
            L +   A+S+WG  Q  NHGIS + L+K+ ++++QFF LP +EK +Y REI   +G+G+
Sbjct: 64  ELSKLHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGN 123

Query: 127 DLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAM 186
           D+ILS+ Q+LDW DRLYL+  PED+R+LKFWPENP  FRE LHE+T+K + ++E    A+
Sbjct: 124 DMILSDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKAL 183

Query: 187 AASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEG 246
           A SL++E   F +  G+   L TRFN YPPC  P  VLGLK HSDGSAFT++L DK VEG
Sbjct: 184 ARSLELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEG 243

Query: 247 LEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCC 306
           L+  KD +WY+  +  +  ++LINVG+  E+MSNGI+KSPVHR V N +++RI VA FC 
Sbjct: 244 LQFLKDGKWYKASI--LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCN 303

Query: 307 PEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            ++DKEI+P+ GL+ E RPRL++ VK   E+ +F YYQ+G+R ++   I
Sbjct: 304 ADEDKEIQPLNGLVSEARPRLYKAVKK-SEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of Carg27194 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.9e-94
Identity = 169/343 (49.27%), Postives = 233/343 (67.93%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIYKGGY-SGGDSNNDPLPMAEIPVVDLS-QLSSSQGGEAA 66
           P+    Q+++  G   PE Y+        G   N  +P+ +IP +DLS  LS S  G   
Sbjct: 4   PKFKTVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREE 63

Query: 67  LEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSD 126
           L +   ALS+WG  Q  NHGI+ + L+K+ +++++F ALP EEK +Y REI   +GYG+D
Sbjct: 64  LSKLHSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGND 123

Query: 127 LILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMA 186
           +IL + Q+LDW DRLY+   PED+R+LKFWP+ P  FRE LHE+T+K   +   V  AMA
Sbjct: 124 MILWDDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMA 183

Query: 187 ASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGL 246
            SL++E   F D  G+   + TRFN YPPC  P  V+G++ H+D SAFT++L DK VEGL
Sbjct: 184 ISLELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGL 243

Query: 247 EMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCP 306
           +  KD +WY+ PV A +D++LINVG+Q EIMSNGI+KSPVHR VTN+E++RISVA FC P
Sbjct: 244 QFLKDGKWYKAPVVA-SDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIP 303

Query: 307 EKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAV 348
             DKEI+P++GL+ E RPRL++ VKNYV +   +YY +GQR +
Sbjct: 304 GADKEIQPVDGLVSEARPRLYKPVKNYV-DLLNKYYIQGQRPI 344

[Close]

BLAST of Carg27194 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 7.2e-94
Identity = 165/344 (47.97%), Postives = 239/344 (69.48%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIY-KGGYSGGDSNNDPLPMAEIPVVDLSQL-SSSQGGEAA 66
           P+    Q+++  G   PE Y++   G       N  +P  +IP +DLS L SSS  G+  
Sbjct: 4   PKFKTVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEE 63

Query: 67  LEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSD 126
           +++   ALS+WG  Q  NHGI+ +FL+K+ ++++QFFALP EEK++  RE    +GYG+D
Sbjct: 64  MKKLHSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGND 123

Query: 127 LILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMA 186
           +ILS+ Q+LDW DRL+L   PED+R+LKFWP+ P  F E L E+T+K + +IE    AMA
Sbjct: 124 MILSDNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMA 183

Query: 187 ASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGL 246
            SL++E   F +  G+  V+ +RFNF+PPC  P  V+G+K H+DGSA T++L DK+VEGL
Sbjct: 184 RSLELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGL 243

Query: 247 EMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCP 306
           +  KD +WY+ P+  + D++LI +G+Q EIMSNGI+KSPVHR VTN E++RISVA FC P
Sbjct: 244 QFLKDGKWYKAPI--VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVP 303

Query: 307 EKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVD 349
             DKEI P +GL+ E RPRL++TV  YV + +++YYQ+G+R ++
Sbjct: 304 GLDKEIHPADGLVTEARPRLYKTVTKYV-DLHYKYYQQGRRTIE 344

[Close]

BLAST of Carg27194 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 9.8e-59
Identity = 121/313 (38.66%), Postives = 186/313 (59.42%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRL--ALSSWGCFQATNHGISSSFLEKMRQISEQFFAL 105
           +IPV+DLS+LS     +   E  +L  A   WG FQ  NHGI    +E + +++ +FF +
Sbjct: 54  QIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 113

Query: 106 PMEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFRE 165
           P+EEK +Y  E    +GYG   I SE Q LDW +   L V+P   R  K WP  P  F E
Sbjct: 114 PLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSE 173

Query: 166 DLHEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGL 225
            L  ++ +++E+ + +L  +A SL ++ + F +  G+  V   R N+YPPCS+P LVLGL
Sbjct: 174 SLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGE-AVQAVRMNYYPPCSSPDLVLGL 233

Query: 226 KEHSDGSAFTIVLLDK-EVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKS 285
             HSDGSA T++   K    GL++ KDN W  +PV  + ++L+IN+G+  E++SNG +KS
Sbjct: 234 SPHSDGSALTVLQQSKNSCVGLQILKDNTW--VPVKPLPNALVINIGDTIEVLSNGKYKS 293

Query: 286 PVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLI-DERRPRLFRTVKNYVEETYFQYYQ 345
             HRAVTN E++R+++  F  P  + EI+P+  L+ DE  P  +R+  N+ + +Y     
Sbjct: 294 VEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSY-NHGDYSYHYVSN 353

Query: 346 K--GQRAVDELKI 353
           K  G++++D  KI
Sbjct: 354 KLQGKKSLDFAKI 362

The following BLAST results are available for this feature:

BLAST of Carg27194 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587867.1	1.4e-203	100.00	Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021754.1 Prot...	[more]
XP_011655698.2	1.5e-157	79.13	probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653028....	[more]
XP_008465534.1	1.6e-156	77.46	PREDICTED: protein SRG1-like [Cucumis melo]	[more]
TYK10347.1	6.2e-156	77.81	protein SRG1-like [Cucumis melo var. makuwa]	[more]
XP_011655280.1	1.1e-155	77.97	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucumis sativus] >...	[more]

BLAST of Carg27194 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39224	9.9e-56	35.90	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
A2A1A0	1.8e-52	38.69	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]
O80449	5.1e-52	35.66	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q94LP4	5.6e-51	33.73	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]
D4N502	1.3e-50	38.52	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]

BLAST of Carg27194 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CP07	7.9e-157	77.46	protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1	[more]
A0A5D3CEG2	3.0e-156	77.81	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00...	[more]
A0A5A7U2F3	2.0e-155	77.53	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00...	[more]
A0A6J1BYU1	7.4e-155	77.62	uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A0A0LW96	2.3e-132	76.17	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212830 PE=4 SV=1	[more]

BLAST of Carg27194 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20400.1	2.6e-99	50.72	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	3.5e-96	48.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20550.1	1.9e-94	49.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	7.2e-94	47.97	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	9.8e-59	38.66	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..156 e-value: 7.2E-25 score: 88.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 206..304 e-value: 4.9E-20 score: 71.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 17..342 e-value: 1.1E-87 score: 296.4
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 13..347
None	No IPR available	PANTHER	PTHR47991:SF63	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 13..347
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..341
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 202..304 score: 11.181284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27194-RA	Carg27194-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity