Carg27069 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27069
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein MIZU-KUSSEI 1-like
Location	Carg_Chr20: 11134250 .. 11135032 (+)
RNA-Seq Expression	Carg27069
Synteny	Carg27069

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCGTATTGCTTCATTACCGTCGGTTCATGGGAGACCCAAAAACCACCTCCACCGCCATCATTGCGATAGATTCGTCAGCTCCACCGCCGCCTCCGCCACCGCCGCCAAAAGCCCCGTCGCTATTGCAATCCACCTCCCTCGTTCCCCCATCCAAGAGAAAGAAGAACAAAGCCAAAGTCTTCCGAGTTTTCCGATCGGTTTTCCGATCATTCCCCATCATAACTCCGGCATGCAAGATCCCCCCACTCCCCTCCGGGATAACAGAAACGCTCCGAACGCCGTCGGGGAGCCGCGTGACCGGGACGCTCTTCGGGTACCGAAAAGGGCGCGTGAGCTTATCGATGCAAGAGACTCCACGGAGTTTCCCCAGCATTGTGATCGAGCTAGCCATGCAGACCAACGTGCTGCAGAGGGAGATGAGCTCGGGGATGGTTAGGATCGCGCTTGAGTGCGAGAAGAGAACCGACAAGGCCGACAAGACCAAGCTCATGGAGGAGCCGTTATGGTCCATGTTTTGTAACGGTAAGAAAACTGGGTATGGCGTTAAAAGGGCAGCCTCCGATGAAGATCTTAAAGTCATGGAGATTCTCCGCCCGGTGTCCATGGGTGCCGGAGTGTTGCCGGGAAACTCTGACGTGGAGGGCCCCGATGGTGAGATGGCATACATGAGGGCCCACTTTGAACGGGTTGTGGGGTCCAGGGATTCGGAGACGTTTTACATGTTGAGCCCGGAGGAAAATAATGGGCCGGAGCTTAGTATCTTTTTTGTAAGAATTTAA

mRNA sequence

ATGACCGTATTGCTTCATTACCGTCGGTTCATGGGAGACCCAAAAACCACCTCCACCGCCATCATTGCGATAGATTCGTCAGCTCCACCGCCGCCTCCGCCACCGCCGCCAAAAGCCCCGTCGCTATTGCAATCCACCTCCCTCGTTCCCCCATCCAAGAGAAAGAAGAACAAAGCCAAAGTCTTCCGAGTTTTCCGATCGGTTTTCCGATCATTCCCCATCATAACTCCGGCATGCAAGATCCCCCCACTCCCCTCCGGGATAACAGAAACGCTCCGAACGCCGTCGGGGAGCCGCGTGACCGGGACGCTCTTCGGGTACCGAAAAGGGCGCGTGAGCTTATCGATGCAAGAGACTCCACGGAGTTTCCCCAGCATTGTGATCGAGCTAGCCATGCAGACCAACGTGCTGCAGAGGGAGATGAGCTCGGGGATGGTTAGGATCGCGCTTGAGTGCGAGAAGAGAACCGACAAGGCCGACAAGACCAAGCTCATGGAGGAGCCGTTATGGTCCATGTTTTGTAACGGTAAGAAAACTGGGTATGGCGTTAAAAGGGCAGCCTCCGATGAAGATCTTAAAGTCATGGAGATTCTCCGCCCGGTGTCCATGGGTGCCGGAGTGTTGCCGGGAAACTCTGACGTGGAGGGCCCCGATGGTGAGATGGCATACATGAGGGCCCACTTTGAACGGGTTGTGGGGTCCAGGGATTCGGAGACGTTTTACATGTTGAGCCCGGAGGAAAATAATGGGCCGGAGCTTAGTATCTTTTTTGTAAGAATTTAA

Coding sequence (CDS)

ATGACCGTATTGCTTCATTACCGTCGGTTCATGGGAGACCCAAAAACCACCTCCACCGCCATCATTGCGATAGATTCGTCAGCTCCACCGCCGCCTCCGCCACCGCCGCCAAAAGCCCCGTCGCTATTGCAATCCACCTCCCTCGTTCCCCCATCCAAGAGAAAGAAGAACAAAGCCAAAGTCTTCCGAGTTTTCCGATCGGTTTTCCGATCATTCCCCATCATAACTCCGGCATGCAAGATCCCCCCACTCCCCTCCGGGATAACAGAAACGCTCCGAACGCCGTCGGGGAGCCGCGTGACCGGGACGCTCTTCGGGTACCGAAAAGGGCGCGTGAGCTTATCGATGCAAGAGACTCCACGGAGTTTCCCCAGCATTGTGATCGAGCTAGCCATGCAGACCAACGTGCTGCAGAGGGAGATGAGCTCGGGGATGGTTAGGATCGCGCTTGAGTGCGAGAAGAGAACCGACAAGGCCGACAAGACCAAGCTCATGGAGGAGCCGTTATGGTCCATGTTTTGTAACGGTAAGAAAACTGGGTATGGCGTTAAAAGGGCAGCCTCCGATGAAGATCTTAAAGTCATGGAGATTCTCCGCCCGGTGTCCATGGGTGCCGGAGTGTTGCCGGGAAACTCTGACGTGGAGGGCCCCGATGGTGAGATGGCATACATGAGGGCCCACTTTGAACGGGTTGTGGGGTCCAGGGATTCGGAGACGTTTTACATGTTGAGCCCGGAGGAAAATAATGGGCCGGAGCTTAGTATCTTTTTTGTAAGAATTTAA

Protein sequence

MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAKVFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTGYGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETFYMLSPEENNGPELSIFFVRI

Homology

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BLAST of Carg27069 vs. NCBI nr
Match: KAG7011160.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.5 bits (1316), Expect = 4.2e-141
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

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BLAST of Carg27069 vs. NCBI nr
Match: XP_022928020.1 (protein MIZU-KUSSEI 1-like [Cucurbita moschata] >KAG6571394.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 507.3 bits (1305), Expect = 7.9e-140
Identity = 257/260 (98.85%), Postives = 259/260 (99.62%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPP+APSLLQSTSLVPPSKRKKNK K
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPQAPSLLQSTSLVPPSKRKKNKTK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPS+VIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSMVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

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BLAST of Carg27069 vs. NCBI nr
Match: XP_022971651.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 506.9 bits (1304), Expect = 1.0e-139
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNK K
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKTK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPS+VIELAM TNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSMVIELAMHTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

[Close]

BLAST of Carg27069 vs. NCBI nr
Match: XP_023513195.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.1 bits (1294), Expect = 1.5e-138
Identity = 255/260 (98.08%), Postives = 257/260 (98.85%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSS PPPPPPPPP+APSLLQSTSLVPPSKRKKNK K
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSGPPPPPPPPPQAPSLLQSTSLVPPSKRKKNKTK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPS+VIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSMVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAAS EDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASGEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

[Close]

BLAST of Carg27069 vs. NCBI nr
Match: XP_008457962.1 (PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo])

HSP 1 Score: 438.7 bits (1127), Expect = 3.4e-119
Identity = 223/262 (85.11%), Postives = 239/262 (91.22%), Query Frame = 0


Query: 11  MGDPKTTSTAIIAID------------SSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNK 70
           MGDPK+TSTAI+ I             SSAPPPPPPPPP++ S   S+SLVPPSKRKKNK
Sbjct: 1   MGDPKSTSTAIVTISPTTSLDPLSAYCSSAPPPPPPPPPQSSSSSSSSSLVPPSKRKKNK 60

Query: 71  AKVFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQE 130
            KVFRVFRSVFRSFPIITPACKIP LPSG+++TLR PSGSRVTGTLFGYRKGRVSLS+QE
Sbjct: 61  TKVFRVFRSVFRSFPIITPACKIPSLPSGLSDTLRGPSGSRVTGTLFGYRKGRVSLSIQE 120

Query: 131 TPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKK 190
           TPRSFPSIVIELAMQTNVLQ+EMSSGMVRIALECEKR DK+DKTKLM+EPLW+MFCNGKK
Sbjct: 121 TPRSFPSIVIELAMQTNVLQKEMSSGMVRIALECEKRADKSDKTKLMDEPLWTMFCNGKK 180

Query: 191 TGYGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSE 250
           TGYGVKR ASDEDLKVME+LRPVSMGAGVLPGNSD+EGPDGE+AYMRAHFERVVGSRDSE
Sbjct: 181 TGYGVKREASDEDLKVMELLRPVSMGAGVLPGNSDMEGPDGELAYMRAHFERVVGSRDSE 240

Query: 251 TFYMLSPEENNGPELSIFFVRI 261
           TFYMLSPEENNGPELSIFFVRI
Sbjct: 241 TFYMLSPEENNGPELSIFFVRI 262

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BLAST of Carg27069 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.2e-44
Identity = 108/234 (46.15%), Postives = 142/234 (60.68%), Query Frame = 0


Query: 52  SKRKKNKAKVFRVFRSVFRSF--PIITPACKIPPLPSGITETLRTPS------------- 111
           S    N  K    F  + RSF   I  PACK+  LPS  + +    +             
Sbjct: 66  SSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSL 125

Query: 112 GSRVTGTLFGYRKGRVSLSMQETPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRT 171
           G RVTGTL+G+++G V+ S+Q   RS P ++++LAM T  L +EMSSG+VRIALECEKR 
Sbjct: 126 GRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR- 185

Query: 172 DKADKTKLMEEPLWSMFCNGKKTGYGVKR--AASDEDLKVMEILRPVSMGAGVLPGN--- 231
                TKL +EP W+M+CNG+K GY V R  A +D D +V+  +  V++GAGV+P     
Sbjct: 186 -HRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTI 245

Query: 232 SDVEGPD-----GEMAYMRAHFERVVGSRDSETFYMLSPEENNGPELSIFFVRI 261
            DV G       GE+ YMR  FERVVGSRDSE FYM++P++N GPELSIF +RI
Sbjct: 246 DDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

[Close]

BLAST of Carg27069 vs. ExPASy TrEMBL
Match: A0A6J1EQH5 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434930 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 3.8e-140
Identity = 257/260 (98.85%), Postives = 259/260 (99.62%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPP+APSLLQSTSLVPPSKRKKNK K
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPQAPSLLQSTSLVPPSKRKKNKTK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPS+VIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSMVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

[Close]

BLAST of Carg27069 vs. ExPASy TrEMBL
Match: A0A6J1I3V4 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470333 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.0e-140
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAK 60
           MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNK K
Sbjct: 1   MTVLLHYRRFMGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKTK 60

Query: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120
           VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP
Sbjct: 61  VFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETP 120

Query: 121 RSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180
           RSFPS+VIELAM TNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG
Sbjct: 121 RSFPSMVIELAMHTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTG 180

Query: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240
           YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF
Sbjct: 181 YGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETF 240

Query: 241 YMLSPEENNGPELSIFFVRI 261
           YMLSPEENNGPELSIFFVRI
Sbjct: 241 YMLSPEENNGPELSIFFVRI 260

[Close]

BLAST of Carg27069 vs. ExPASy TrEMBL
Match: A0A1S3C801 (protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103497527 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.7e-119
Identity = 223/262 (85.11%), Postives = 239/262 (91.22%), Query Frame = 0


Query: 11  MGDPKTTSTAIIAID------------SSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNK 70
           MGDPK+TSTAI+ I             SSAPPPPPPPPP++ S   S+SLVPPSKRKKNK
Sbjct: 1   MGDPKSTSTAIVTISPTTSLDPLSAYCSSAPPPPPPPPPQSSSSSSSSSLVPPSKRKKNK 60

Query: 71  AKVFRVFRSVFRSFPIITPACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQE 130
            KVFRVFRSVFRSFPIITPACKIP LPSG+++TLR PSGSRVTGTLFGYRKGRVSLS+QE
Sbjct: 61  TKVFRVFRSVFRSFPIITPACKIPSLPSGLSDTLRGPSGSRVTGTLFGYRKGRVSLSIQE 120

Query: 131 TPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKK 190
           TPRSFPSIVIELAMQTNVLQ+EMSSGMVRIALECEKR DK+DKTKLM+EPLW+MFCNGKK
Sbjct: 121 TPRSFPSIVIELAMQTNVLQKEMSSGMVRIALECEKRADKSDKTKLMDEPLWTMFCNGKK 180

Query: 191 TGYGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSE 250
           TGYGVKR ASDEDLKVME+LRPVSMGAGVLPGNSD+EGPDGE+AYMRAHFERVVGSRDSE
Sbjct: 181 TGYGVKREASDEDLKVMELLRPVSMGAGVLPGNSDMEGPDGELAYMRAHFERVVGSRDSE 240

Query: 251 TFYMLSPEENNGPELSIFFVRI 261
           TFYMLSPEENNGPELSIFFVRI
Sbjct: 241 TFYMLSPEENNGPELSIFFVRI 262

[Close]

BLAST of Carg27069 vs. ExPASy TrEMBL
Match: A0A6J1L383 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499488 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 218/255 (85.49%), Postives = 233/255 (91.37%), Query Frame = 0


Query: 11  MGDPKTTSTAIIAID----SSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAKVFRVFR 70
           MGDPK+T+ A+  +     SSAPP PPP PP+ PSL  S+SLVPPSKRKKNK KVFRVFR
Sbjct: 1   MGDPKSTA-ALPELQSPPKSSAPPQPPPLPPQ-PSLTSSSSLVPPSKRKKNKTKVFRVFR 60

Query: 71  SVFRSFPIITP-ACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETPRSFPS 130
           SVFRSFPIITP ACKIP LPSGI+ETL T SG+RVTGTLFGYRKGRVSLS+QETPRSFPS
Sbjct: 61  SVFRSFPIITPAACKIPSLPSGISETLLTQSGNRVTGTLFGYRKGRVSLSIQETPRSFPS 120

Query: 131 IVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTGYGVKR 190
           IVIELAMQTNVLQ+EMSSGMVRIALECEKRTDKADKTKLMEEPLW+MFCNGKK GYGVKR
Sbjct: 121 IVIELAMQTNVLQKEMSSGMVRIALECEKRTDKADKTKLMEEPLWTMFCNGKKNGYGVKR 180

Query: 191 AASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETFYMLSP 250
            ASD+DLKVME+LR VSMGAGVLPGNSDV+GPDGE AYMRAHFERVVGSRDSETFYMLSP
Sbjct: 181 EASDDDLKVMELLRAVSMGAGVLPGNSDVDGPDGEQAYMRAHFERVVGSRDSETFYMLSP 240

Query: 251 EENNGPELSIFFVRI 261
           EEN+GPELSIFFVRI
Sbjct: 241 EENSGPELSIFFVRI 253

[Close]

BLAST of Carg27069 vs. ExPASy TrEMBL
Match: A0A6J1G801 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451590 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 218/255 (85.49%), Postives = 233/255 (91.37%), Query Frame = 0


Query: 11  MGDPKTTSTAIIAID----SSAPPPPPPPPPKAPSLLQSTSLVPPSKRKKNKAKVFRVFR 70
           MGDPK+T+ A+  +     SSAPP PPP PP+ PSL  S+SLVPPSKRKKNK KVFRVFR
Sbjct: 1   MGDPKSTA-ALPELQSPPKSSAPPQPPPLPPQ-PSLTSSSSLVPPSKRKKNKTKVFRVFR 60

Query: 71  SVFRSFPIITP-ACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETPRSFPS 130
           SVFRSFPIITP ACKIP LPSGI+ETL T SG+RVTGTLFGYRKGRVSLS+QETPRSFPS
Sbjct: 61  SVFRSFPIITPAACKIPSLPSGISETLLTQSGNRVTGTLFGYRKGRVSLSIQETPRSFPS 120

Query: 131 IVIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTGYGVKR 190
           IVIELAMQTNVLQ+EMSSGMVRIALECEKRTDKADKTKLMEEPLW+MFCNGKK GYGVKR
Sbjct: 121 IVIELAMQTNVLQKEMSSGMVRIALECEKRTDKADKTKLMEEPLWTMFCNGKKNGYGVKR 180

Query: 191 AASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETFYMLSP 250
            ASD+DLKVME+LR VSMGAGVLPGNSDV+GPDGE AYMRAHFERVVGSRDSETFYMLSP
Sbjct: 181 EASDDDLKVMELLRAVSMGAGVLPGNSDVDGPDGEQAYMRAHFERVVGSRDSETFYMLSP 240

Query: 251 EENNGPELSIFFVRI 261
           EEN+GPELSIFFVRI
Sbjct: 241 EENSGPELSIFFVRI 253

[Close]

BLAST of Carg27069 vs. TAIR 10
Match: AT2G21990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 318.5 bits (815), Expect = 4.8e-87
Identity = 176/254 (69.29%), Postives = 206/254 (81.10%), Query Frame = 0


Query: 11  MGDPKTTSTAIIAIDSSAPPPPPPPPPKAPSLLQSTSLVPPS-KRKKNKAKVFRVFRSVF 70
           MG+ +  S A  + DS + P P   P  +P+  Q  +L+ PS + KK   KVFRVFRSVF
Sbjct: 1   MGEQR-ASGASSSTDSYSTPSPSASPSPSPAPRQHVTLLEPSHQHKKKSKKVFRVFRSVF 60

Query: 71  RSFPIITP-ACKIPPLPSG-ITETLRT-PSGSRVTGTLFGYRKGRVSLSMQETPRSFPSI 130
           RSFPIITP ACKIP LP G + +  R+  SGSRVTGTLFGYRKGRVSLS+QE+PR  PS+
Sbjct: 61  RSFPIITPAACKIPVLPGGSLPDQHRSGSSGSRVTGTLFGYRKGRVSLSIQESPRCLPSL 120

Query: 131 VIELAMQTNVLQREMSSGMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTGYGVKRA 190
           V+ELAMQT VLQ+E+S GMVRIALE EKR DK +K K+M+EPLW+MF NGKKTGYGVKR 
Sbjct: 121 VVELAMQTMVLQKELSGGMVRIALETEKRGDK-EKIKIMDEPLWTMFSNGKKTGYGVKRD 180

Query: 191 ASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVGSRDSETFYMLSPE 250
           A++EDL VME+LRPVSMGAGVLPGN++ EGPD EMAYMRA+FERVVGS+DSETFYMLSPE
Sbjct: 181 ATEEDLNVMELLRPVSMGAGVLPGNTEFEGPDSEMAYMRAYFERVVGSKDSETFYMLSPE 240

Query: 251 ENNGPELSIFFVRI 261
            NNGPELSIFFVR+
Sbjct: 241 GNNGPELSIFFVRV 252

[Close]

BLAST of Carg27069 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 294.7 bits (753), Expect = 7.5e-80
Identity = 164/267 (61.42%), Postives = 198/267 (74.16%), Query Frame = 0


Query: 13  DPKTTSTAIIAIDSSAPPPPPPP-------PPKAPSLLQSTSLVPPSKRKKNKAKVFRVF 72
           +P  TS++  +  SS PP  P P       PP+   LLQ     P SK+KKN+  VFRV 
Sbjct: 6   NPMATSSS--SSSSSIPPSSPTPSPTSTTTPPRQHPLLQP----PSSKKKKNRTNVFRVL 65

Query: 73  RSVFRSFPIITP---ACKIPPLPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETPRS 132
           R+VFRSFPI T    ACKIP +  G+       + SR+TGTLFGYRKGRVSLS+QE P+ 
Sbjct: 66  RTVFRSFPIFTTPSVACKIPVIHPGLGLPDPHHNTSRITGTLFGYRKGRVSLSIQENPKC 125

Query: 133 FPSIVIELAMQTNVLQREMSSGMVRIALECEK---------RTDKADKTKLMEEPLWSMF 192
            PS+V+ELAMQT  LQ+E+S+GMVRIALE EK         +T+K  KT ++EEPLW+M+
Sbjct: 126 LPSLVVELAMQTTTLQKELSTGMVRIALETEKQPRADNNNSKTEK--KTDILEEPLWTMY 185

Query: 193 CNGKKTGYGVKRAASDEDLKVMEILRPVSMGAGVLPGNSDVEGPDGEMAYMRAHFERVVG 252
           C G+KTGYGVKR A++EDL VME+LRPVSMGAGVLPGNS+ EGPDGEMAYMRA+FERV+G
Sbjct: 186 CKGEKTGYGVKREATEEDLNVMELLRPVSMGAGVLPGNSESEGPDGEMAYMRAYFERVIG 245

Query: 253 SRDSETFYMLSPEENNGPELSIFFVRI 261
           S+DSETFYMLSPE NNGPELS FFVR+
Sbjct: 246 SKDSETFYMLSPEGNNGPELSFFFVRV 264

[Close]

BLAST of Carg27069 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 228.4 bits (581), Expect = 6.6e-60
Identity = 123/247 (49.80%), Postives = 167/247 (67.61%), Query Frame = 0


Query: 28  APPPPPPPPPKAPSLLQSTSLVPPSKRKK----NKAKVFRVFRSVFRSFPIITPACKIPP 87
           +P   P       ++    +L  PS R K       K+FR  RSVFRS PI++P CK P 
Sbjct: 17  SPLGSPKSKKSTATVRPEITLEQPSGRNKTTGSKSTKLFRRVRSVFRSLPIMSPMCKFPV 76

Query: 88  LPSGITETLRTPSGSRVTGTLFGYRKGRVSLSMQETPRSFPSIVIELAMQTNVLQREMSS 147
              G         G+RVTGTLFGYRK RV+L++QE PRS P +++ELA+ T  L +++  
Sbjct: 77  GGGGRLHENHVHGGTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGV 136

Query: 148 GMVRIALECEKRTDKADKTKLMEEPLWSMFCNGKKTGYGVKRAASDEDLKVMEILRPVSM 207
           G+VRIALECEK+   ++KTK+++EP+W+++CNGKK+GYGVKR  ++EDL VM++L  VSM
Sbjct: 137 GLVRIALECEKK--PSEKTKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSM 196

Query: 208 GAGVLP----------GNSDVEGPDGEMAYMRAHFERVVGSRDSETFYMLSPEENNGPEL 261
           GAGVLP          G       +G++ YMRAHFERV+GSRDSET+YM++P+ N+GPEL
Sbjct: 197 GAGVLPVSSGAITEQSGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPEL 256

[Close]

BLAST of Carg27069 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 180.6 bits (457), Expect = 1.6e-45
Identity = 108/234 (46.15%), Postives = 142/234 (60.68%), Query Frame = 0


Query: 52  SKRKKNKAKVFRVFRSVFRSF--PIITPACKIPPLPSGITETLRTPS------------- 111
           S    N  K    F  + RSF   I  PACK+  LPS  + +    +             
Sbjct: 66  SSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSL 125

Query: 112 GSRVTGTLFGYRKGRVSLSMQETPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRT 171
           G RVTGTL+G+++G V+ S+Q   RS P ++++LAM T  L +EMSSG+VRIALECEKR 
Sbjct: 126 GRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR- 185

Query: 172 DKADKTKLMEEPLWSMFCNGKKTGYGVKR--AASDEDLKVMEILRPVSMGAGVLPGN--- 231
                TKL +EP W+M+CNG+K GY V R  A +D D +V+  +  V++GAGV+P     
Sbjct: 186 -HRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTI 245

Query: 232 SDVEGPD-----GEMAYMRAHFERVVGSRDSETFYMLSPEENNGPELSIFFVRI 261
            DV G       GE+ YMR  FERVVGSRDSE FYM++P++N GPELSIF +RI
Sbjct: 246 DDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

[Close]

BLAST of Carg27069 vs. TAIR 10
Match: AT5G42680.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 170.2 bits (430), Expect = 2.1e-42
Identity = 100/225 (44.44%), Postives = 143/225 (63.56%), Query Frame = 0


Query: 41  SLLQSTSLVPPSK-RKKNKAKVFRVFRSVFRSFPIITPACKIPPLPSGITETLRTP--SG 100
           SLL+ T     SK  KK+   +      +F+  P+++  CK+  L   ++   R P    
Sbjct: 18  SLLRHTGTSMDSKSSKKSSGSIGGGVLKMFKLIPMLSSGCKMVNL---LSRGHRRPLLKD 77

Query: 101 SRVTGTLFGYRKGRVSLSMQETPRSFPSIVIELAMQTNVLQREMSSGMVRIALECEKRTD 160
              TGT+FG+RKGRV L++QE P   P  +IEL M T+ LQ+EM+S  VRIALE E +T 
Sbjct: 78  YATTGTIFGFRKGRVFLAIQEDPHCLPIFIIELPMLTSALQKEMASETVRIALESETKT- 137

Query: 161 KADKTKLMEEPLWSMFCNGKKTGYGVKRA-ASDEDLKVMEILRPVSMGAGVLP-GNSDVE 220
              + K++EE +W ++CNG+K GY ++R   S+E++ V++ LR VSMGAGVLP  N   +
Sbjct: 138 --SRKKVLEEYVWGIYCNGRKIGYSIRRKNMSEEEMYVIDALRGVSMGAGVLPCKNQYDQ 197

Query: 221 GPDGEMAYMRAHFERVVGSRDSETFYMLSPE-ENNGPELSIFFVR 260
             +GEM YMRA F+RV+GS+DSE  YM++PE    G ELSI+F+R
Sbjct: 198 ETEGEMTYMRARFDRVIGSKDSEALYMINPEGSGQGTELSIYFLR 236

The following BLAST results are available for this feature:

BLAST of Carg27069 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011160.1	4.2e-141	100.00	Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022928020.1	7.9e-140	98.85	protein MIZU-KUSSEI 1-like [Cucurbita moschata] >KAG6571394.1 Protein MIZU-KUSSE...	[more]
XP_022971651.1	1.0e-139	98.85	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
XP_023513195.1	1.5e-138	98.08	protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008457962.1	3.4e-119	85.11	PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo]	[more]

BLAST of Carg27069 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	2.2e-44	46.15	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of Carg27069 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EQH5	3.8e-140	98.85	protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434930 PE=4 SV...	[more]
A0A6J1I3V4	5.0e-140	98.85	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470333 PE=4 SV=1	[more]
A0A1S3C801	1.7e-119	85.11	protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103497527 PE=4 SV=1	[more]
A0A6J1L383	1.3e-111	85.49	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499488 PE=4 SV=1	[more]
A0A6J1G801	1.3e-111	85.49	protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451590 PE=4 SV...	[more]

BLAST of Carg27069 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21990.1	4.8e-87	69.29	Protein of unknown function, DUF617	[more]
AT4G39610.1	7.5e-80	61.42	Protein of unknown function, DUF617	[more]
AT5G06990.1	6.6e-60	49.80	Protein of unknown function, DUF617	[more]
AT2G41660.1	1.6e-45	46.15	Protein of unknown function, DUF617	[more]
AT5G42680.1	2.1e-42	44.44	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 100..259 e-value: 5.8E-71 score: 237.8
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 100..260 e-value: 2.1E-76 score: 253.3
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PANTHER	PTHR31696	PROTEIN MIZU-KUSSEI 1	coord: 27..260
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 26..41
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 25..54
None	No IPR available	PANTHER	PTHR31696:SF4	PROTEIN MIZU-KUSSEI 1	coord: 27..260

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27069-RA	Carg27069-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism